def createSbatchFile(self):
# Create batch file in working directory
# From analysis get :
# queue
# expected memory
# number of cores
# time to allocate
cwd = self.analysis.output_dir
name = self.analysis.name
self.batchfile = FileUtils.getDatestampedFilename(
cwd, "PogPipe." + name, ".sbatch")
jobname = "PogPipe." + name
jobout = os.path.join(cwd, jobname + ".out")
joberr = os.path.join(cwd, jobname + ".err")
queue = self.analysis.queue
cores = self.analysis.cores
mempercore = self.analysis.mempercore
time = "120"
# Contents of slurm script is something like
#
# #!/usr/bin/env bash
# #SBATCH -J myRjob <- the analysis number
# #SBATCH -o myRjob_slurm.out <- full path here
# #SBATCH -e myRjob_slurm.err <- full path here
# #SBATCH -p informatics-dev <- queue from analysis
# #SBATCH -n 1 <- number of cores?
# #SBATCH -t 5 <- time in minutes
# #SBATCH --mem=100 <- memory in Mb
str = "#!/usr/bin/env bash\n"
str += "#SBATCH =J " + jobname + "\n"
str += "#SBATCH -o " + jobout + "\n"
str += "#SBATCH -e " + joberr + "\n"
str += "#SBATCH -p " + queue + "\n"
str += "#SBATCH -n %d" % (cores) + "\n"
str += "#SBATCH -t %s" % (time) + "\n"
str += "#SBATCH --mem=%d" % (mempercore) + "\n"
# If we have an analysis id we're reading from the db. If not then we're on the command line
if self.analysis.id is None:
str += "python " + settings.POGPIPEROOT + "bin/run_analysis.py -a " + name + " -i "
str += self.analysis.input_files[0].input_file
else:
str += "python " + settings.POGPIPEROOT + "bin/run_analysis.py -a " + name + " -i "
str += self.analysis.input_files[0].input_file
self.batchfiletext = str
FileUtils.writeTextToFile(self.batchfile, str)
def checkDiskSpace(self):
bytes = FileUtils.getFreeDiskSpace(self.working_dir)
logging.info(" ========> Analysis %20s checked disk space for %s Free space (bytes) %s Needed %s"%(self.name,self.working_dir,bytes,self.minimum_space_needed))
if self.minimum_space_needed and bytes < self.minimum_space_needed:
str = "Not enough disk space needed in %s. Needs %s, available %s"%(self.working_dir,self.minimum_space_needed,bytes)
logging.info(" ========> Analysis %20s %s"%(self.name,str))
raise Exception(str)
elif bytes < 10000000:
str = "Not enough disk space needed in %s. Needs %s, available %s"%(self.working_dir,self.minimum_space_needed,bytes)
logging.info(" ========> Analysis %20s %s"%(self.name,str))
raise Exception(str)
bytes = FileUtils.getFreeDiskSpace(self.output_dir)
logging.info(" ========> Analysis %20s checked disk space for %s Free space (bytes) %s Needed %s"%(self.name,self.output_dir,bytes,self.minimum_space_needed))
if self.minimum_space_needed and bytes < self.minimum_space_needed:
str = "Not enough disk space needed in %s. Needs %s, available %s"%(self.output_dir,self.minimum_space_needed,bytes)
logging.info(" ========> Analysis %20s %s"%(self.name,str))
raise Exception(str)
elif bytes < 10000000:
str = "Not enough disk space needed in %s. Needs %s, available %s"%(self.output_dir,self.minimum_space_needed,bytes)
logging.info(" ========> Analysis %20s %s"%(self.name,str))
raise Exception(str)
def checkDiskSpace(anaobj):
print anaobj.currentstatus
print anaobj.working_dir
bytes = FileUtils.getFreeDiskSpace(anaobj.working_dir)
logging.info(" ========> Analysis %20s checked disk space for %s Free space (bytes) %s Needed %s"%(anaobj.name,anaobj.working_dir,bytes,anaobj.minimum_space_needed))
if anaobj.minimum_space_needed and bytes < anaobj.minimum_space_needed:
str = "Not enough disk space needed in %s. Needs %s, available %s"%(anaobj.working_dir,anaobj.minimum_space_needed,bytes)
logging.info(" ========> Analysis %20s %s"%(anaobj.name,str))
raise Exception(str)
elif bytes < 10000000:
str = "Not enough disk space needed in %s. Needs %s, available %s"%(anaobj.working_dir,anaobj.minimum_space_needed,bytes)
logging.info(" ========> Analysis %20s %s"%(anaobj.name,str))
raise Exception(str)
bytes = FileUtils.getFreeDiskSpace(anaobj.output_dir)
logging.info(" ========> Analysis %20s checked disk space for %s Free space (bytes) %s Needed %s"%(anaobj.name,anaobj.output_dir,bytes,anaobj.minimum_space_needed))
if anaobj.minimum_space_needed and bytes < anaobj.minimum_space_needed:
str = "Not enough disk space needed in %s. Needs %s, available %s"%(anaobj.output_dir,anaobj.minimum_space_needed,bytes)
logging.info(" ========> Analysis %20s %s"%(anaobj.name,str))
raise Exception(str)
elif bytes < 10000000:
str = "Not enough disk space needed in %s. Needs %s, available %s"%(anaobj.output_dir,anaobj.minimum_space_needed,bytes)
logging.info(" ========> Analysis %20s %s"%(anaobj.name,str))
raise Exception(str)
def testGetFreeGbDiskSpace(self):
"""Check we can find the free disk space for our working directory"""
out = FileUtils.getFreeGbDiskSpace("/tmp/")
print " GBytes free are %d" % out
self.assertTrue(out > 0)
def testGetDiskUsage(self):
"""Check we can find the free disk space for our working directory"""
out = FileUtils.getDiskUsage("/tmp/")
print " Disk usage is %s " % out
self.assertTrue(out > 0)
def testGetPercentFreeDiskSpace(self):
"""Check we can find the free disk space for our working directory"""
out = FileUtils.getPercentFreeDiskSpace("/tmp/")
print " Percent free is %d" % out
self.assertTrue(out >= 0 and out <= 100)
def postProcessOutput(anaobj):
""" Checks expected output files exist in the working directory and copies them over to the output directory """
logging.info(" ========> Analysis %20s called postProcessOutput:"%(anaobj.name))
if AnalysisUtils.checkExpectedOutputFiles(anaobj) == False:
raise Exception("Missing expected output files. Number missing are [%d]"%(len(anaobj.missing_output_files)))
FileUtils.checkDirExists(anaobj.output_dir)
tmpfiles = []
logging.info(" ========> Analysis %20s called postProcessOutput: Moving files from %s to %s "%(anaobj.name,anaobj.working_dir,anaobj.output_dir))
try:
for srcfileobj in anaobj.expected_output_files:
srcfile = srcfileobj.expected_output_file
fullsrcfile = os.path.join(anaobj.working_dir,srcfile)
destfile = os.path.join(anaobj.output_dir,srcfile)
FileUtils.checkDirExistsForFile(destfile)
res = shutil.move(fullsrcfile,destfile)
if res == None:
res = "OK"
else:
res = "FAILED"
print "Checking %s"%destfile
tmpfiles.append(destfile)
logging.info(" ========> Analysis %20s called postProcessOutput: Result of file move for %s = %s" % (anaobj.name,srcfile,res))
except Exception as e:
logging.info(" ========> Analysis %20s file move failed %s"%(anaobj.name,e))
raise
anaobj.output_files = []
for i in tmpfiles:
AnalysisUtils.addOutputFile(anaobj,i)
for f in anaobj.temp_output_files:
logging.info(" ========> Analysis %20s removing temp file %s "%(anaobj.name,f))
res = os.remove(f)
def setUp(self):
if FileUtils.fileExists(settings.TESTDBNAME): os.remove(settings.TESTDBNAME)
settings.DBNAME = settings.TESTDBNAME
init_database()
Session = sessionmaker(bind=settings.ENGINE)
self.session = Session()
def __init__(self, deltafile, refseqs, qryseqs):
self.deltafile = deltafile
self.refseqs = refseqs
self.qryseqs = qryseqs
if not FileUtils.fileExists(self.deltafile):
raise Exception(
"Can't parse Mummer delta file. File [%s] doesn't exist" %
self.deltafile)
def getCommands(self):
self.commands = []
self.output_files = []
self.checkDiskSpace()
seqs = FastaFile.getSequenceDict(self.refgenome, False)
if self.checkInputFiles() == False:
raise Exception("Input files [%s] don't exist = can't continue" %
(self.input_files))
fileparts = FileUtils.getFileParts(self.input_files[0])
self.basename = fileparts['basename']
# Need to set dbtype somewhere
outfile1 = self.working_dir + "/" + self.basename + ".raw.vcf"
outfile2 = self.working_dir + "/" + self.basename + ".flt.vcf"
regstr = ""
if self.regionstr != "":
regstr = " -r " + self.regionstr
outfile1 = self.working_dir + "/" + self.basename + "." + self.regionstr + ".raw.vcf"
outfile2 = self.working_dir + "/" + self.basename + "." + self.regionstr + ".flt.vcf"
self.expected_output_files.append(outfile1)
self.expected_output_files.append(outfile2)
command1 = self.samtools + " mpileup -uf " + self.refgenome + " " + self.input_files[
0] + " " + regstr + " | " + self.bcftools + " view " + " -bvcg - > " + outfile1
command2 = self.bcftools + " view " + outfile1 + " | " + self.vcfutils + " varFilter -D100 > " + outfile2
print "Command %s" % command1
print "Command %s" % command2
self.commands.append(
AnalysisCommand(command=command1,
command_rank=len(self.commands) + 1))
self.commands.append(
AnalysisCommand(command=command2,
command_rank=len(self.commands) + 1))
return self.commands
def getCommands(self):
self.commands = []
self.output_files = []
self.checkDiskSpace()
print "Reading genome file"
seqs = FastaFile.getSequenceDict(self.refgenome,False)
if self.checkInputFiles() == False:
raise Exception("Input files [%s] don't exist = can't continue"%(self.input_files))
fileparts = FileUtils.getFileParts(self.input_files[0])
self.basename = fileparts['basename']
for seq in seqs:
len = seqs[seq]['len']
i = 1
while i < len:
end = i + self.chunk -1
if end > len:
end = len
regionstr = "%s:%d-%d"%(seq,i,end)
tmpana = AnalysisFactory.createAnalysisFromModuleName("SamtoolsMpileup")
tmpana.setInputFiles(self.input_files,self.input_types)
tmpana.refgenome = self.refgenome
tmpana.regionstr = regionstr
tmpana.init()
tmpcmds = tmpana.getCommands()
for cmd in tmpcmds:
self.commands.append(cmd)
i = i + self.chunk
return self.commands
def setUp(self):
if FileUtils.fileExists(settings.TESTDBNAME):
os.remove(settings.TESTDBNAME)
settings.DBNAME = settings.TESTDBNAME
init_database()
#input_files = ['pog1.fa','pog2.fa','pog3.fa']
#input_types = ['fasta','fasta','fasta']
#self.ana = Analysis("FastQC")
self.input_files = ["testdata/FoxP2_SL167.fastq"]
self.input_types = ['fastq']
Session = sessionmaker(bind=settings.ENGINE)
self.session = Session()
def testRunMummer(self):
mummer = Mummer()
self.assertTrue(mummer)
self.assertTrue(AnalysisUtils.setInputFiles(mummer,self.input_files,['fasta','fasta']))
runner = AnalysisRunner(mummer)
self.assertTrue(runner.run())
self.assertTrue(len(mummer.output_strings) == 1)
self.assertTrue(mummer.output_strings[0].output_string.index('4: FINISHING DATA') > 0)
self.assertTrue(FileUtils.fileExists('../testout/mummer.delta'))
def getCommands(self):
self.checkDiskSpace()
if self.checkInputFiles() == False:
raise Exception("Input files [%s] don't exist = can't continue" %
(self.input_files))
fileparts = FileUtils.getFileParts(self.input_files[0])
self.basename = fileparts['basename']
# Need to set dbtype somewhere
command = self.makeblastdb + " -in " + self.input_files[
0] + " -input_type fasta -dbtype prot -title " + self.basename + " -parse_seqids -out " + fileparts[
'dirname'] + "/" + self.basename
print "Command %s" % command
self.commands.append(command)
return self.commands
def init(self):
super(FastQCAnalysis, self).init()
if len(self.input_files) == 0:
raise Exception(
"No input files for FastQCAnalysis module. Can't init")
fileparts = FileUtils.getFileParts(self.input_files[0].input_file)
if fileparts['fileext'] == ".fastq":
dir = fileparts['filestub'] + "_fastqc/"
elif fileparts['fileext'] == ".gz":
dir = fileparts['filestub'].replace(".fastq", "") + "_fastqc/"
else:
dir = fileparts['basename'] + "_fastqc/"
self.fastqc_dir = dir
tmp = []
for i, f in enumerate(self.expected_output_filelist):
#tmp.append(dir + f)
AnalysisUtils.addExpectedOutputFile(self, dir + f)
def tearDown(self):
deltafile = '../testout/mummer.delta'
if FileUtils.fileExists(deltafile):
os.remove(deltafile)
def init(self):
if len(self.input_files) == 0:
raise Exception("No input files for BlastOutput6Parsermodule. Can't init")
fileparts = FileUtils.getFileParts(self.input_files[0])
def testGetFilesInDirectory(self):
"""Check we can return an array of files in a directory"""
files = FileUtils.getAllFilesInDirectory("/tmp/")
self.assertTrue(len(files) > 0)
def checkBinary(self,binfile):
return FileUtils.fileExists(binfile)
"""Unit test for Analysis.py"""
import os
import sys
import unittest
import logging
sys.path.append(os.path.dirname(os.path.realpath(__file__)) + "/../")
from config import settings
from datamodel.FileUtils import FileUtils
from datamodel.database.DB import Analysis, init_database
from datamodel.database.AnalysisUtils import AnalysisUtils
from sqlalchemy.orm import sessionmaker
if FileUtils.fileExists(settings.TESTLOGFILE): os.remove(settings.TESTLOGFILE)
formatter = logging.Formatter(
'%(asctime)s - %(name)s - %(levelname)s - %(message)s')
fh = logging.FileHandler(settings.TESTLOGFILE, 'a')
fh.setFormatter(formatter)
log = logging.getLogger() # root logger
for hdlr in log.handlers: # remove all old handlers
log.removeHandler(hdlr)
log.addHandler(fh) # set the new handler
class DBAnalysisTest(unittest.TestCase
def getCommands(self):
if self.commands and len(self.commands) > 0:
return self.commands
logging.info(" ========> Analysis %20s Getting commands" % (self.name))
self.commands = []
self.expected_output_files = []
self.temp_output_files = []
outdir = self.output_dir
tmpdir = self.working_dir
btbin = self.bowtiebindir + self.bowtiebinname
stbin = self.samtoolsbindir + self.samtoolsbinname
self.calculateSpaceNeeded()
if FileUtils.fileExists(btbin) == False:
raise Exception("Binary file [%s] doesn't exist = can't continue" %
btbin)
if FileUtils.fileExists(stbin) == False:
raise Exception("Binary file [%s] doesn't exist = can't continue" %
stbin)
if AnalysisUtils.checkInputFiles(self) == False:
raise Exception("Input files [%s] don't exist = can't continue" %
(self.input_files))
AnalysisUtils.checkDiskSpace(self)
for fobj in self.input_files:
f = fobj.input_file
try:
if f.endswith(".gz"):
# f = "<( zcat -c " + f + " )"
tmpf = f.replace(".gz", "")
fparts = FileUtils.getFileParts(tmpf)
command = "gunzip -c " + f + " > " + tmpdir + "/" + fparts[
'basename']
self.commands.append(command)
self.temp_output_files.append(tmpf)
f = tmpdir + "/" + fparts['basename']
fparts = FileUtils.getFileParts(f)
fstub = fparts['filestub']
bowtieoutfile = tmpdir + "/" + fstub + ".sam"
samtoolsoutfile = tmpdir + "/" + fstub + ".bam"
if self.param == None:
raise Exception(
"No parameters entered for bowtie = needs -x <genomeindex>"
)
command1 = btbin + " " + self.param + " " + f + " | " + stbin + " view -bS - | " + stbin + " sort - " + tmpdir + "/" + fstub
logging.info(" ========> Analysis %20s command 1 : %s" %
(self.name, command1))
#command2 = stbin + " view -bS " + bowtieoutfile + "| " + stbin + " sort - " + tmpdir + "/" + fstub
# logging.info(" ========> Analysis %20s command 2 : %s" % (self.name,command2))
command2 = stbin + " index " + samtoolsoutfile
logging.info(" ========> Analysis %20s command 3 : %s" %
(self.name, command2))
# self.expected_output_files.append(fstub + ".sam")
self.expected_output_files.append(
AnalysisExpectedOutputFile(expected_output_file=fstub +
".bam"))
self.expected_output_files.append(
AnalysisExpectedOutputFile(expected_output_file=fstub +
".bam.bai"))
self.commands.append(AnalysisCommand(command=command1))
self.commands.append(AnalysisCommand(command=command2))
#self.commands.append(command3)
except Exception as e:
logging.info(
" ========> Analysis %20s Failed building command list [%s]"
% (self.name, e))
raise
return self.commands
