def test_ridge_reducer_fit(spark):
indexdf = spark.read.parquet(f'{data_root}/groupedIDs.snappy.parquet')
blockdf = spark.read.parquet(f'{data_root}/blockedGT.snappy.parquet')
testGroup = '0'
testBlock = 'chr_1_block_0'
ids = indexdf.filter(f'sample_block = {testGroup}').select(
'sample_ids').head().sample_ids
headers = [
r.header for r in blockdf.filter(
f'header_block = "{testBlock}" AND sample_block = {testGroup}').
orderBy('sort_key').select('header').collect()
]
X_out = X0[headers].drop(ids, axis='rows')
Y_out = labeldf.drop(ids, axis='rows')
XtX_out = X_out.to_numpy().T @ X_out.to_numpy()
XtY_out = X_out.to_numpy().T @ Y_out.to_numpy()
B = np.column_stack([
(np.linalg.inv(XtX_out + np.identity(XtX_out.shape[1]) * a) @ XtY_out)
for a in alphas
])
stack = RidgeReducer(alphas)
modeldf = stack.fit(blockdf, labeldf, __get_sample_blocks(indexdf))
columns = ['coefficients']
rows = modeldf.filter(f'header_block = "{testBlock}" AND sample_block = {testGroup}') \
.select(*columns).collect()
outdf = pd.DataFrame(rows, columns=columns)
B_stack = np.row_stack(outdf['coefficients'].to_numpy())
assert np.allclose(B_stack, B)
def test_reducer_fit_transform(spark):
indexdf = spark.read.parquet(f'{data_root}/groupedIDs.snappy.parquet')
blockdf = spark.read.parquet(f'{data_root}/blockedGT.snappy.parquet').limit(5)
group2ids = __get_sample_blocks(indexdf)
stack0 = RidgeReducer(alphas)
model0df = stack0.fit(blockdf, labeldf, group2ids)
level1df = stack0.transform(blockdf, labeldf, group2ids, model0df)
fit_transform_df = stack0.fit_transform(blockdf, labeldf, group2ids)
__assert_dataframes_equal(fit_transform_df, level1df)
def test_ridge_reducer_transform_with_cov(spark):
indexdf = spark.read.parquet(f'{data_root}/groupedIDs.snappy.parquet')
blockdf = spark.read.parquet(f'{data_root}/blockedGT.snappy.parquet')
testGroup = '0'
testBlock = 'chr_1_block_0'
ids = indexdf.filter(f'sample_block = {testGroup}').select(
'sample_ids').head().sample_ids
sample_blocks = __get_sample_blocks(indexdf)
headers = [
r.header for r in blockdf.filter(
f'header_block = "{testBlock}" AND sample_block= {testGroup}').
orderBy('sort_key').select('header').collect()
]
C_in = covdf.loc[ids, :].values
X_in = X0[headers].loc[ids, :].values
X_in_cov = np.column_stack([C_in, X_in])
C_out = covdf.drop(ids, axis='rows').values
X_out = X0[headers].drop(ids, axis='rows').values
X_out_cov = np.column_stack((C_out, X_out))
Y_out = labeldf.drop(ids, axis='rows').values
XtX_out_cov = X_out_cov.T @ X_out_cov
XtY_out_cov = X_out_cov.T @ Y_out
diags_cov = [
np.concatenate(
[np.ones(n_cov),
np.ones(XtX_out_cov.shape[1] - n_cov) * a]) for a in alphas
]
B_cov = np.column_stack([
(np.linalg.inv(XtX_out_cov + np.diag(d)) @ XtY_out_cov)
for d in diags_cov
])
X1_in_cov = X_in_cov @ B_cov
stack = RidgeReducer(alphas)
modeldf_cov = stack.fit(blockdf, labeldf, sample_blocks, covdf)
level1df_cov = stack.transform(blockdf, labeldf, sample_blocks,
modeldf_cov, covdf)
columns = ['alpha', 'label', 'values']
rows_cov = level1df_cov.filter(f'header LIKE "%{testBlock}%" AND sample_block= {testGroup}') \
.select(*columns) \
.collect()
outdf_cov = pd.DataFrame(rows_cov, columns=columns)
X1_in_stack_cov = np.column_stack(outdf_cov['values'])
assert np.allclose(X1_in_stack_cov, X1_in_cov)
def test_one_level_regression(spark):
indexdf = spark.read.parquet(f'{data_root}/groupedIDs.snappy.parquet')
blockdf = spark.read.parquet(f'{data_root}/blockedGT.snappy.parquet')
testLabel = 'sim100'
group2ids = __get_sample_blocks(indexdf)
bestAlpha, bestr2, y_hat = __calculate_y_hat(X1, group2ids, testLabel)
stack0 = RidgeReducer(alphas)
model0df = stack0.fit(blockdf, labeldf, group2ids)
level1df = stack0.transform(blockdf, labeldf, group2ids, model0df)
regressor = RidgeRegression(alphas)
model1df, cvdf = regressor.fit(level1df, labeldf, group2ids)
yhatdf = regressor.transform(level1df, labeldf, group2ids, model1df, cvdf)
r = cvdf.filter(f'label = "{testLabel}"').select('alpha', 'r2_mean').head()
bestAlpha_lvl, bestr2_lvl = (r.alpha, r.r2_mean)
y_hat_lvl = np.array(yhatdf[testLabel])
assert (bestAlpha_lvl == bestAlpha and np.isclose(bestr2_lvl, bestr2)
and np.allclose(y_hat_lvl, np.array(y_hat)))
def test_reducer_generate_alphas(spark):
indexdf = spark.read.parquet(f'{data_root}/groupedIDs.snappy.parquet')
blockdf = spark.read.parquet(f'{data_root}/blockedGT.snappy.parquet').limit(5)
group2ids = __get_sample_blocks(indexdf)
stack_without_alphas = RidgeReducer()
stack_with_alphas = RidgeReducer(np.array(sorted(list(generate_alphas(blockdf).values()))))
model0_without_alphas = stack_without_alphas.fit(blockdf, labeldf, group2ids)
model0df = stack_with_alphas.fit(blockdf, labeldf, group2ids)
__assert_dataframes_equal(model0_without_alphas, model0df)
level1_without_alphas = stack_without_alphas.transform(blockdf, labeldf, group2ids, model0df)
level1df = stack_with_alphas.transform(blockdf, labeldf, group2ids, model0df)
__assert_dataframes_equal(level1_without_alphas, level1df)
def test_reducer_missing_alphas(spark):
indexdf = spark.read.parquet(f'{data_root}/groupedIDs.snappy.parquet')
blockdf = spark.read.parquet(f'{data_root}/blockedGT.snappy.parquet').limit(5)
group2ids = __get_sample_blocks(indexdf)
stack_fit = RidgeReducer()
stack_transform = RidgeReducer()
model0df = stack_fit.fit(blockdf, labeldf, group2ids)
level1df = stack_transform.transform(blockdf, labeldf, group2ids, model0df)
with pytest.raises(Exception):
level1df.collect()
def test_reducer_transform_validates_inputs(spark):
indexdf = spark.read.parquet(f'{data_root}/groupedIDs.snappy.parquet')
blockdf = spark.read.parquet(
f'{data_root}/blockedGT.snappy.parquet').limit(5)
group2ids = __get_sample_blocks(indexdf)
reducer = RidgeReducer(alphas)
model0df = reducer.fit(blockdf, labeldf, group2ids)
with pytest.raises(ValueError):
reducer.transform(blockdf, label_with_missing, group2ids, model0df,
covdf)
with pytest.raises(ValueError):
reducer.transform(blockdf, labeldf, group2ids, model0df,
covdf_with_missing)
with pytest.warns(UserWarning):
reducer.transform(blockdf, labeldf + 0.5, group2ids, model0df, covdf)
with pytest.warns(UserWarning):
reducer.transform(blockdf, labeldf * 1.5, group2ids, model0df, covdf)
with pytest.warns(UserWarning):
reducer.transform(blockdf, labeldf, group2ids, model0df, covdf + 0.5)
with pytest.warns(UserWarning):
reducer.transform(blockdf, labeldf, group2ids, model0df, covdf * 1.5)
# Should issue no warnings
reducer.transform(blockdf, labeldf, group2ids, model0df, covdf)
def run(
plink_path: str,
traits_path: str,
covariates_path: str,
variants_per_block: int,
sample_block_count: int,
output_dir: str,
plink_fam_sep: str = "\t",
plink_bim_sep: str = "\t",
alphas: Optional[list] = None,
contigs: List[str] = None,
):
"""Run Glow WGR"""
output_path = Path(output_dir)
if output_path.exists():
shutil.rmtree(output_path)
output_path.mkdir(parents=True, exist_ok=False)
if alphas is None:
alphas = np.array([])
else:
alphas = np.array(alphas).astype(float)
spark = spark_session()
logger.info(
f"Loading PLINK dataset at {plink_path} (fam sep = {plink_fam_sep}, bim sep = {plink_bim_sep}, alphas = {alphas})"
)
df = (spark.read.format("plink").option(
"bimDelimiter",
plink_bim_sep).option("famDelimiter", plink_fam_sep).option(
"includeSampleIds", True).option("mergeFidIid",
False).load(plink_path))
variant_df = df.withColumn(
"values", mean_substitute(genotype_states(F.col("genotypes")))).filter(
F.size(F.array_distinct("values")) > 1)
if contigs is not None:
variant_df = variant_df.filter(F.col("contigName").isin(contigs))
sample_ids = get_sample_ids(variant_df)
logger.info(
f"Found {len(sample_ids)} samples, first 10: {sample_ids[:10]}")
###########
# Stage 1 #
###########
logger.info(HR)
logger.info("Calculating variant/sample block info")
block_df, sample_blocks = block_variants_and_samples(
variant_df,
sample_ids,
variants_per_block=variants_per_block,
sample_block_count=sample_block_count,
)
label_df = pd.read_csv(traits_path, index_col="sample_id")
label_df = (label_df - label_df.mean()) / label_df.std(ddof=0)
logger.info(HR)
logger.info("Trait info:")
logger.info(_info(label_df))
cov_df = pd.read_csv(covariates_path, index_col="sample_id")
cov_df = (cov_df - cov_df.mean()) / cov_df.std(ddof=0)
logger.info(HR)
logger.info("Covariate info:")
logger.info(_info(cov_df))
stack = RidgeReducer(alphas=alphas)
reduced_block_df = stack.fit_transform(block_df, label_df, sample_blocks,
cov_df)
logger.info(HR)
logger.info("Stage 1: Reduced block schema:")
logger.info(_schema(reduced_block_df))
path = output_path / "reduced_blocks.parquet"
reduced_block_df.write.parquet(str(path), mode="overwrite")
logger.info(f"Stage 1: Reduced blocks written to {path}")
# Flatten to scalars for more convenient access w/o Spark
flat_reduced_block_df = spark.read.parquet(str(path))
path = output_path / "reduced_blocks_flat.csv.gz"
flat_reduced_block_df = _flatten_reduced_blocks(flat_reduced_block_df)
flat_reduced_block_df = flat_reduced_block_df.toPandas()
flat_reduced_block_df.to_csv(path, index=False)
# flat_reduced_block_df.write.parquet(str(path), mode='overwrite')
logger.info(f"Stage 1: Flattened reduced blocks written to {path}")
###########
# Stage 2 #
###########
# Monkey-patch this in until there's a glow release beyond 0.5.0
if glow_version != "0.5.0":
raise NotImplementedError(
f"Must remove adjustements for glow != 0.5.0 (found {glow_version})"
)
# Remove after glow update
RidgeRegression.transform_loco = transform_loco
estimator = RidgeRegression(alphas=alphas)
model_df, cv_df = estimator.fit(reduced_block_df, label_df, sample_blocks,
cov_df)
logger.info(HR)
logger.info("Stage 2: Model schema:")
logger.info(_schema(model_df))
logger.info("Stage 2: CV schema:")
logger.info(_schema(cv_df))
y_hat_df = estimator.transform(reduced_block_df, label_df, sample_blocks,
model_df, cv_df, cov_df)
logger.info(HR)
logger.info("Stage 2: Prediction info:")
logger.info(_info(y_hat_df))
logger.info(y_hat_df.head(5))
path = output_path / "predictions.csv"
y_hat_df.reset_index().to_csv(path, index=False)
logger.info(f"Stage 2: Predictions written to {path}")
y_hat_df_loco = estimator.transform_loco(reduced_block_df, label_df,
sample_blocks, model_df, cv_df,
cov_df)
path = output_path / "predictions_loco.csv"
y_hat_df_loco.reset_index().to_csv(path, index=False)
logger.info(f"Stage 2: LOCO Predictions written to {path}")
###########
# Stage 3 #
###########
# Do this to correct for the error in Glow at https://github.com/projectglow/glow/issues/257
if glow_version != "0.5.0":
raise NotImplementedError(
f"Must remove adjustements for glow != 0.5.0 (found {glow_version})"
)
cov_arr = cov_df.to_numpy()
cov_arr = cov_arr.T.ravel(order="C").reshape(cov_arr.shape)
# Convert the pandas dataframe into a Spark DataFrame
adjusted_phenotypes = reshape_for_gwas(spark, label_df - y_hat_df)
# Run GWAS w/o LOCO (this could be for a much larger set of variants)
wgr_gwas = (variant_df.withColumnRenamed("values", "callValues").crossJoin(
adjusted_phenotypes.withColumnRenamed(
"values", "phenotypeValues")).select(
"start",
"names",
"label",
expand_struct(
linear_regression_gwas(F.col("callValues"),
F.col("phenotypeValues"),
F.lit(cov_arr))),
))
logger.info(HR)
logger.info("Stage 3: GWAS (no LOCO) schema:")
logger.info(_schema(wgr_gwas))
# Convert to pandas
wgr_gwas = wgr_gwas.toPandas()
logger.info(HR)
logger.info("Stage 3: GWAS (no LOCO) info:")
logger.info(_info(wgr_gwas))
logger.info(wgr_gwas.head(5))
path = output_path / "gwas.csv"
wgr_gwas.to_csv(path, index=False)
logger.info(f"Stage 3: GWAS (no LOCO) results written to {path}")
logger.info(HR)
logger.info("Done")
# TODO: Enable this once WGR is fully released
# See: https://github.com/projectglow/glow/issues/256)
# Run GWAS w/ LOCO
adjusted_phenotypes = reshape_for_gwas(spark, label_df - y_hat_df_loco)
wgr_gwas = (variant_df.withColumnRenamed("values", "callValues").join(
adjusted_phenotypes.withColumnRenamed("values", "phenotypeValues"),
["contigName"],
).select(
"contigName",
"start",
"names",
"label",
expand_struct(
linear_regression_gwas(F.col("callValues"),
F.col("phenotypeValues"), F.lit(cov_arr))),
))
# Convert to pandas
wgr_gwas = wgr_gwas.toPandas()
logger.info(HR)
logger.info("Stage 3: GWAS (with LOCO) info:")
logger.info(_info(wgr_gwas))
logger.info(wgr_gwas.head(5))
path = output_path / "gwas_loco.csv"
wgr_gwas.to_csv(path, index=False)
logger.info(f"Stage 3: GWAS (with LOCO) results written to {path}")
logger.info(HR)
logger.info("Done")