def discover(args):
datafile = args.datafile
outputfile = args.output
paramsfile = args.params
sample_req = args.sample
sample_flag = False #used to check whether sample_req exists
print 'Loading data file...'
dataloader = DataManager(datafile)
params = dataloader.getParams(paramsfile)
dataloader.skipHeadline()
sample = dataloader.getNextSample()
targets_list = dataloader.getTargetsList()
output = file(outputfile, 'w')
output.write(
'SAMPLE_ID\tCNV\tFULL_INTERVAL\tINDEX\tINTERVAL\tREAD_DEPTH\n')
while sample:
if sample_req == '' or (sample_req != ''
and sample['sample_id'] == sample_req):
sample_flag = True
#target_index is used to split observations sequence
target_index_begin = 0
target_index_end = 0
temp = 1
for targets in targets_list:
print 'Running HMM for sample[' + sample['sample_id'] + ']: ',
print 'chr' + targets[0]._chr + ' [' + str(temp) + '\\' + str(
len(targets_list)) + ']'
temp += 1
target_index_end = target_index_begin + len(targets)
modelParams = ModelParams(params, targets)
#the 'observations' of sample is splitted
model = Model(
modelParams, sample['observations']
[target_index_begin:target_index_end])
pathlist = model.forwardBackward_Viterbi()
dataloader.outputCNV(
output, sample['sample_id'], targets, pathlist,
sample['observations']
[target_index_begin:target_index_end])
target_index_begin = target_index_end
sample = dataloader.getNextSample()
output.close()
dataloader.closeFile()
if not sample_flag:
print 'Could not find the sample_id specified.'
def discover(args) :
datafile = args.datafile
outputfile = args.output
paramsfile = args.params
sample_req = args.sample
sample_flag = False #used to check whether sample_req exists
print 'Loading data file...'
dataloader = DataManager(datafile)
params = dataloader.getParams(paramsfile)
dataloader.skipHeadline()
sample = dataloader.getNextSample()
targets_list = dataloader.getTargetsList()
output = file(outputfile, 'w')
output.write('SAMPLE_ID\tCNV\tFULL_INTERVAL\tINDEX\tINTERVAL\tREAD_DEPTH\n')
while sample :
if sample_req == '' or (sample_req != '' and sample['sample_id'] == sample_req):
sample_flag = True
#target_index is used to split observations sequence
target_index_begin = 0
target_index_end = 0
temp = 1
for targets in targets_list:
print 'Running HMM for sample[' + sample['sample_id'] + ']: ',
print 'chr' + targets[0]._chr + ' [' + str(temp) + '\\' + str(len(targets_list)) + ']'
temp += 1
target_index_end = target_index_begin + len(targets)
modelParams = ModelParams(params, targets)
#the 'observations' of sample is splitted
model = Model(modelParams, sample['observations'][target_index_begin:target_index_end])
pathlist = model.forwardBackward_Viterbi()
dataloader.outputCNV(output, sample['sample_id'], targets, pathlist, sample['observations'][target_index_begin:target_index_end])
target_index_begin = target_index_end
sample = dataloader.getNextSample()
output.close()
dataloader.closeFile()
if not sample_flag:
print 'Could not find the sample_id specified.'
conf_prob,trans_prob=pro._tran_conf_prob(train,test_wordcount,word_count,hidden_states)
'''
test = [["中华人民共和国今天成立了中国人民从此站起来了"],
["江泽民的三个代表是中国在社会主义改革过程中的智慧结晶"],
["人民日报称改革开发的伟大旗帜要坚定不移动的走下去"],
["日理万机的周总理"],
["国务院今天颁发了关于农业的改革方向前进步伐"],
["机器学习及其翻译激起了人们极其浓厚的兴趣"],
["中共中央书记"]]
observations = pro._str2words(test)
'''
observations = test
phi = {'B':0.5,'E':0,'M':0,'S':0.5}
model = Model(S,observation,phi,trans_prob,conf_prob)
o_hstate = []
for obser in observations:
'''
Notice,if a setence is too long,when we use viterbi algorithm it may result in the beta = 0
There are two solution,one is split the setence into serval sub_setence,another is use log function for the viterbi
here we select the first method
'''
length = len(obser)
index,sub_obser,state= 0,[],[]
while index < length:
sub_obser.append(obser[index])
if obser[index] == '。' or obser[index]=='，':
sub_state = model.decode(sub_obser)
sub_obser = []
# -*- coding: utf-8 -*-
from hmm import Model
states = ("rainy", "sunny")
symbols = ("walk", "shop", "clean")
start_prob = {"rainy": 0.5, "sunny": 0.5}
trans_prob = {"rainy": {"rainy": 0.7, "sunny": 0.3}, "sunny": {"rainy": 0.4, "sunny": 0.6}}
emit_prob = {"rainy": {"walk": 0.1, "shop": 0.4, "clean": 0.5}, "sunny": {"walk": 0.6, "shop": 0.3, "clean": 0.1}}
sequence = ["walk", "shop", "clean", "clean", "walk", "walk", "walk", "clean"]
model = Model(states, symbols, start_prob, trans_prob, emit_prob)
print model.evaluate(sequence)
print model.decode(sequence)
trans_prob = {
'rainy': {
'rainy': 0.7,
'sunny': 0.3
},
'sunny': {
'rainy': 0.4,
'sunny': 0.6
}
}
emit_prob = {
'rainy': {
'walk': 0.1,
'shop': 0.4,
'clean': 0.5
},
'sunny': {
'walk': 0.6,
'shop': 0.3,
'clean': 0.1
}
}
sequence = ['walk', 'shop', 'clean', 'clean', 'walk', 'walk', 'walk', 'clean']
model = Model(states, symbols, start_prob, trans_prob, emit_prob)
print model.evaluate(sequence)
print model.decode(sequence)
outputfile = 'output'
paramsfile = 'params.txt'
print 'Loading data file...'
dataloader = DataManager(datafile)
params = dataloader.getParams(paramsfile)
dataloader.skipHeadline()
sample = dataloader.getNextSample()
targets_list = dataloader.getTargetsList()
output = file(outputfile, 'w')
while sample :
#target_index is used to split observations sequence
target_index_begin = 0
target_index_end = 0
temp = 1
for targets in targets_list:
print 'Running HMM for sample[' + sample['sample_id'] + ']: ',
print 'chr' + targets[0]._chr + ' [' + str(temp) + '\\' + str(len(targets_list)) + ']'
temp += 1
target_index_end = target_index_begin + len(targets)
modelParams = ModelParams(params, targets)
#the 'observations' of sample is splitted
model = Model(modelParams, sample['observations'][target_index_begin:target_index_end])
pathlist = model.forwardBackward_Viterbi()
dataloader.outputCNV(output, sample['sample_id'], targets, pathlist, sample['observations'][target_index_begin:target_index_end])
target_index_begin = target_index_end
sample = dataloader.getNextSample()
output.close()
#The hidden states
states = [1,2,3]
#The observation states
observation = [1,2]
#The intial probability for the hidden states
phi = {1:0.333,2:0.333,3:0.333}
#The trans prob for the hidden states
trans_prob = {
1:{1:0.333,2:0.333,3:0.333},
2:{1:0.333,2:0.333,3:0.333},
3:{1:0.333,2:0.333,3:0.333}
}
#The prob of observation in condition of a hidden state
conf_prob = {
1:{1:0.5,2:0.5},
2:{1:0.75,2:0.25},
3:{1:0.25,2:0.75}
}
observations =[1,1,1,1,2,1,2,2,2,2]
model = Model(states,observation,phi,trans_prob,conf_prob)
print model.evaluate(observations)
print model.decode(observations)
emit_prob = {
'rainy': {
'walk': 0.1,
'shop': 0.4,
'clean': 0.5
},
'sunny': {
'walk': 0.6,
'shop': 0.3,
'clean': 0.1
}
}
sequence = ['walk', 'shop', 'clean', 'clean', 'walk', 'walk', 'walk', 'clean']
sequence2 = ['walk', 'walk', 'walk', 'walk', 'walk', 'walk', 'walk', 'walk']
model = Model(states, symbols, start_prob, trans_prob, emit_prob)
print(model.evaluate(sequence))
for i in range(0, len(sequence)):
print(i)
print(
model._forward(sequence)[i]['sunny'] *
model._backward(sequence)[i]['sunny'] +
model._forward(sequence)[i]['rainy'] *
model._backward(sequence)[i]['rainy'])
print(model.decode(sequence))
print(model.decode(sequence2))
print(model._forward(sequence))
conf_prob, trans_prob = pro._tran_conf_prob(train, test_wordcount, word_count,
hidden_states)
'''
test = [["中华人民共和国今天成立了中国人民从此站起来了"],
["江泽民的三个代表是中国在社会主义改革过程中的智慧结晶"],
["人民日报称改革开发的伟大旗帜要坚定不移动的走下去"],
["日理万机的周总理"],
["国务院今天颁发了关于农业的改革方向前进步伐"],
["机器学习及其翻译激起了人们极其浓厚的兴趣"],
["中共中央书记"]]
observations = pro._str2words(test)
'''
observations = test
phi = {'B': 0.5, 'E': 0, 'M': 0, 'S': 0.5}
model = Model(S, observation, phi, trans_prob, conf_prob)
o_hstate = []
for obser in observations:
'''
Notice,if a setence is too long,when we use viterbi algorithm it may result in the beta = 0
There are two solution,one is split the setence into serval sub_setence,another is use log function for the viterbi
here we select the first method
'''
length = len(obser)
index, sub_obser, state = 0, [], []
while index < length:
sub_obser.append(obser[index])
if obser[index] == '。' or obser[index] == '，':
sub_state = model.decode(sub_obser)
sub_obser = []
params = dataloader.getParams(paramsfile)
dataloader.skipHeadline()
sample = dataloader.getNextSample()
targets_list = dataloader.getTargetsList()
output = file(outputfile, 'w')
while sample:
#target_index is used to split observations sequence
target_index_begin = 0
target_index_end = 0
temp = 1
for targets in targets_list:
print 'Running HMM for sample[' + sample['sample_id'] + ']: ',
print 'chr' + targets[0]._chr + ' [' + str(temp) + '\\' + str(
len(targets_list)) + ']'
temp += 1
target_index_end = target_index_begin + len(targets)
modelParams = ModelParams(params, targets)
#the 'observations' of sample is splitted
model = Model(
modelParams,
sample['observations'][target_index_begin:target_index_end])
pathlist = model.forwardBackward_Viterbi()
dataloader.outputCNV(
output, sample['sample_id'], targets, pathlist,
sample['observations'][target_index_begin:target_index_end])
target_index_begin = target_index_end
sample = dataloader.getNextSample()
output.close()
#The prob of observation in condition of a hidden state
conf_prob = {
'rainy': {
'walk': 0.1,
'shop': 0.3,
'clean': 0.6
},
'sunny': {
'walk': 0.4,
'shop': 0.5,
'clean': 0.1
},
'cloudy': {
'walk': 0.6,
'shop': 0.25,
'clean': 0.15
}
}
observations = [
'walk', 'shop', 'clean', 'clean', 'walk', 'walk', 'walk', 'clean'
]
#The iter_num is the iteration number in the EM algorithm
iter_num = 50
model = Model(states, observation, phi, trans_prob, conf_prob, iter_num)
print model.evaluate(observations)
print model.decode(observations)
def discover(args) :
paramsfile = args.params
sample_req = args.sample
hetsnp = args.hetsnp
tagsnp = args.tagsnp
vcf_file = args.vcf
if hetsnp == 'True' or hetsnp == 'TRUE':
hetsnp = True
else:
hetsnp = False
if tagsnp == 'True' or tagsnp == 'TRUE':
tagsnp = True
else:
tagsnp = False
datafile = args.rpkm_matrix
f_dir = os.path.dirname(datafile)
if f_dir != '':
f_dir = f_dir + '/'
if args.output:
outputfile = f_dir + str(args.output)
tagsnp_file = args.tagsnp_file
mode = args.mode
sample_flag = False #used to check whether sample_req exists
# Build a reference set
if mode == 'single' or mode == 'baseline' or mode == 'reference' or mode == 'ref':
print 'Building the reference dataset...'
dataloader = DataManager(datafile)
samples_np = dataloader.getAllSamples()
dataloader.closeFile()
print 'Baseline is Done.'
print 'Loading data file...',
dataloader = DataManager(datafile)
print 'Done!'
print 'Loading paramters...',
params = dataloader.getParams(paramsfile)
print 'Done!'
dataloader.skipHeadline()
sample = dataloader.getNextSample()
targets_list = dataloader.getTargetsList()
output_aux = file(outputfile+'.aux', 'w')
output_aux.write('SAMPLE_ID\tCNV_TYPE\tFULL_INTERVAL\tINDEX\tINTERVAL\tREAD_DEPTH\n')
output = file(outputfile,'w')
output.write('SAMPLE_ID\tCNV_TYPE\tINTERVAL\tCHROMOSOME\tSTART\tSTOP\tLENGTH\n')
if (hetsnp or tagsnp) and vcf_file == '':
print 'Error: please indicate a vcf file!'
system.exit(0)
if vcf_file != '':
vcf_reader = VCFReader(vcf_file)
else:
vcf_reader = False
if tagsnp:
print 'Loading tagSNP information ...',
cnp_dict = vcf_reader.loadTagSNP(tagsnp_file)
print 'Done!'
while sample :
if sample_req == '' or (sample_req != '' and sample['sample_id'] == sample_req):
sample_flag = True
print time.strftime("%Y-%m-%d %H:%M:%S", time.localtime()) ,sample_req,'......'
#Renjie added: To check whether the VCF contains sample_req.
vcf_checker = vcf.Reader(open(vcf_file,'r'))
if sample['sample_id'] in vcf_checker.samples:
sample_in_VCF = True
elif sample_req in vcf_checker.samples:
sample_in_VCF = True
else:
print 'No sample %s in VCF file.'%sample_req
sample_in_VCF = False
if hetsnp and sample_in_VCF :
print 'Parsing SNV information from VCF file for: ' + sample['sample_id']
snp_info = vcf_reader.getSNPInfo(sample['sample_id'], targets_list)
if tagsnp and sample_in_VCF:
print 'Analysing tagSNP information from tagSNP database for: ' + sample['sample_id'],
cnp_list = vcf_reader.findTagSNPForSample(sample['sample_pop'], sample['sample_id'], cnp_dict)
tagsnp_info_list = vcf_reader.findExonWithTagSNP(cnp_list, targets_list, overlap_threshold=0.5)
print len(tagsnp_info_list)
#estimate NB paramters from sample['observations']
sample_observations = []
remove_list = []
sample['observations'] = [ float(x) for x in sample['observations']]
#slicing: target_index is used to split observations sequence
target_index_begin = 0
target_index_end = 0
temp = 1
sample_observations_list = []
snp_info_list = []
for i, targets in enumerate(targets_list):
target_index_end = target_index_begin + len(targets)
if hetsnp and sample_in_VCF:
snp_info_list.append(snp_info[target_index_begin:target_index_end])
sample_observations_list.append(sample['observations'][target_index_begin:target_index_end])
target_index_begin = target_index_end
# Filtering:
if mode == 'svd' or mode == 'SVD' or mode == 'pooled' or mode == 'pooled-sample':
for i in range(len(sample_observations_list)):
sample_observations_list[i] = ndarray.tolist(stats.zscore(sample_observations_list[i]))
elif mode == 'baseline' or mode == 'reference' or mode == 'single' or mode == 'single-sample':
# filtering lists whose observation equals to 0
for i in range(len(targets_list)):
rem_index = []
for j in range(len(targets_list[i])):
value = sample_observations_list[i][j]
if np.isnan(float(value)):
rem_index.append(j)
#filter target_list, snp_list and observation_list
targets_list[i] = jf.filter_list_by_list(targets_list[i], rem_index)
sample_observations_list[i] = jf.filter_list_by_list(sample_observations_list[i], rem_index)
if hetsnp and sample_in_VCF:
snp_info_list[i] = jf.filter_list_by_list(snp_info_list[i], rem_index)
if tagsnp and sample_in_VCF:
tagsnp_info_list[i] = jf.filter_list_by_list(tagsnp_info_list[i], rem_index)
#Parameters estimation
observations_all_list = []
for i in range(len(sample_observations_list)):
observations_all_list.extend(sample_observations_list[i])
parameterLoader = ParameterEstimation(observations_all_list)
parameterList = parameterLoader.fit(observations_all_list,0.01,0.99)
print "Estimated Paramters: ",parameterList
params.append(parameterList[0])#mu
params.append(parameterList[1])#sd
for i, targets in enumerate(targets_list):
print 'Running HMM for sample[' + sample['sample_id'] + ']: ',
print 'chr' + targets[0]._chr + ' [' + str(temp) + '|' + str(len(targets_list)) + ']'
temp += 1
#Run the HMM
if not hetsnp and not tagsnp:
modelParams = ModelParams(mode, params, targets, het_nums=0, tagsnp=0)
elif sample_in_VCF and hetsnp and not tagsnp:
modelParams = ModelParams(mode, params, targets, snp_info_list[i], tagsnp=0)
elif sample_in_VCF and not hetsnp and tagsnp:
modelParams = ModelParams(mode, params, targets, het_nums=0, tagsnp=tagsnp_info_list[i])
elif sample_in_VCF and hetsnp and tagsnp:
modelParams = ModelParams(mode, params, targets, snp_info_list[i], tagsnp_info_list[i])
elif not sample_in_VCF and hetsnp and tagsnp:
modelParams = ModelParams(mode, params, targets, het_nums=0, tagsnp=0)
else:
pdb.set_trace()
model = Model(mode, modelParams, sample_observations_list[i])
pathlist = list()
if vcf_reader and sample_in_VCF:
pathlist = model.forwardBackward_Viterbi(mode, if_snp = True)
else:
pathlist = model.forwardBackward_Viterbi(mode, if_snp = False)
dataloader.outputCNVaux(output_aux, sample['sample_id'], targets, pathlist, sample_observations_list[i])
dataloader.outputCNV(output, sample['sample_id'], targets, pathlist, sample_observations_list[i])
sample = dataloader.getNextSample()
output.close()
output_aux.close()
dataloader.closeFile()
if not sample_flag:
print 'Could not find the sample_id specified.'
