def htg(args):
"""
%prog htg fastafile template.sbt
Prepare sqnfiles for Genbank HTG submission to update existing records.
`fastafile` contains the records to update, multiple records are allowed
(with each one generating separate sqn file in the sqn/ folder). The record
defline has the accession ID. For example,
>AC148290.3
Internally, this generates two additional files (phasefile and namesfile)
and download records from Genbank. Below is implementation details:
`phasefile` contains, for each accession, phase information. For example:
AC148290.3 3 HTG 2 mth2-45h12
which means this is a Phase-3 BAC. Record with only a single contig will be
labeled as Phase-3 regardless of the info in the `phasefile`. Template file
is the Genbank sbt template. See jcvi.formats.sbt for generation of such
files.
Another problem is that Genbank requires the name of the sequence to stay
the same when updating and will kick back with a table of name conflicts.
For example:
We are unable to process the updates for these entries
for the following reason:
Seqname has changed
Accession Old seq_name New seq_name
--------- ------------ ------------
AC239792 mtg2_29457 AC239792.1
To prepare a submission, this script downloads genbank and asn.1 format,
and generate the phase file and the names file (use formats.agp.phase() and
apps.gbsubmit.asn(), respectively). These get automatically run.
However, use --phases if the genbank files contain outdated information.
For example, the clone name changes or phase upgrades. In this case, run
formats.agp.phase() manually, modify the phasefile and use --phases to override.
"""
from jcvi.formats.fasta import sequin, ids
from jcvi.formats.agp import phase
from jcvi.apps.fetch import entrez
p = OptionParser(htg.__doc__)
p.add_option("--phases", default=None,
help="Use another phasefile to override [default: %default]")
p.add_option("--comment", default="",
help="Comments for this update [default: %default]")
opts, args = p.parse_args(args)
if len(args) != 2:
sys.exit(not p.print_help())
fastafile, sbtfile = args
pf = fastafile.rsplit(".", 1)[0]
idsfile = pf + ".ids"
phasefile = pf + ".phases"
namesfile = pf + ".names"
ids([fastafile, "--outfile={0}".format(idsfile)])
asndir = "asn.1"
mkdir(asndir)
entrez([idsfile, "--format=asn.1", "--outdir={0}".format(asndir)])
asn(glob("{0}/*".format(asndir)) + \
["--outfile={0}".format(namesfile)])
if opts.phases is None:
gbdir = "gb"
mkdir(gbdir)
entrez([idsfile, "--format=gb", "--outdir={0}".format(gbdir)])
phase(glob("{0}/*".format(gbdir)) + \
["--outfile={0}".format(phasefile)])
else:
phasefile = opts.phases
assert op.exists(namesfile) and op.exists(phasefile)
newphasefile = phasefile + ".new"
newphasefw = open(newphasefile, "w")
comment = opts.comment
fastadir = "fasta"
sqndir = "sqn"
mkdir(fastadir)
mkdir(sqndir)
from jcvi.graphics.histogram import stem_leaf_plot
names = DictFile(namesfile)
assert len(set(names.keys())) == len(set(names.values()))
phases = DictFile(phasefile)
ph = [int(x) for x in phases.values()]
# vmin 1, vmax 4, bins 3
stem_leaf_plot(ph, 1, 4, 3, title="Counts of phases before updates")
logging.debug("Information loaded for {0} records.".format(len(phases)))
assert len(names) == len(phases)
newph = []
cmd = "faSplit byname {0} {1}/".format(fastafile, fastadir)
sh(cmd, outfile="/dev/null", errfile="/dev/null")
acmd = 'tbl2asn -a z -p fasta -r {sqndir}'
acmd += ' -i {splitfile} -t {sbtfile} -C tigr'
acmd += ' -j "{qualifiers}"'
acmd += ' -A {accession_nv} -o {sqndir}/{accession_nv}.sqn -V Vbr'
acmd += ' -y "{comment}" -W T -T T'
qq = "[tech=htgs {phase}] [organism=Medicago truncatula] [strain=A17]"
nupdated = 0
for row in open(phasefile):
atoms = row.rstrip().split("\t")
# see formats.agp.phase() for column contents
accession, phase, clone = atoms[0], atoms[1], atoms[-1]
fafile = op.join(fastadir, accession + ".fa")
accession_nv = accession.split(".", 1)[0]
newid = names[accession_nv]
newidopt = "--newid={0}".format(newid)
cloneopt = "--clone={0}".format(clone)
splitfile, gaps = sequin([fafile, newidopt, cloneopt])
splitfile = op.basename(splitfile)
phase = int(phase)
assert phase in (1, 2, 3)
oldphase = phase
if gaps == 0 and phase != 3:
phase = 3
if gaps != 0 and phase == 3:
phase = 2
print("{0}\t{1}\t{2}".\
format(accession_nv, oldphase, phase), file=newphasefw)
newph.append(phase)
qualifiers = qq.format(phase=phase)
if ";" in clone:
qualifiers += " [keyword=HTGS_POOLED_MULTICLONE]"
cmd = acmd.format(accession=accession, accession_nv=accession_nv,
sqndir=sqndir, sbtfile=sbtfile, splitfile=splitfile,
qualifiers=qualifiers, comment=comment)
sh(cmd)
verify_sqn(sqndir, accession)
nupdated += 1
stem_leaf_plot(newph, 1, 4, 3, title="Counts of phases after updates")
print("A total of {0} records updated.".format(nupdated), file=sys.stderr)
def htg(args):
"""
%prog htg fastafile template.sbt
Prepare sqnfiles for Genbank HTG submission to update existing records.
`fastafile` contains the records to update, multiple records are allowed
(with each one generating separate sqn file in the sqn/ folder). The record
defline has the accession ID. For example,
>AC148290.3
Internally, this generates two additional files (phasefile and namesfile)
and download records from Genbank. Below is implementation details:
`phasefile` contains, for each accession, phase information. For example:
AC148290.3 3 HTG 2 mth2-45h12
which means this is a Phase-3 BAC. Record with only a single contig will be
labeled as Phase-3 regardless of the info in the `phasefile`. Template file
is the Genbank sbt template. See jcvi.formats.sbt for generation of such
files.
Another problem is that Genbank requires the name of the sequence to stay
the same when updating and will kick back with a table of name conflicts.
For example:
We are unable to process the updates for these entries
for the following reason:
Seqname has changed
Accession Old seq_name New seq_name
--------- ------------ ------------
AC239792 mtg2_29457 AC239792.1
To prepare a submission, this script downloads genbank and asn.1 format,
and generate the phase file and the names file (use formats.agp.phase() and
apps.gbsubmit.asn(), respectively). These get automatically run.
However, use --phases if the genbank files contain outdated information.
For example, the clone name changes or phase upgrades. In this case, run
formats.agp.phase() manually, modify the phasefile and use --phases to override.
"""
from jcvi.formats.fasta import sequin, ids
from jcvi.formats.agp import phase
from jcvi.apps.fetch import entrez
p = OptionParser(htg.__doc__)
p.add_option(
"--phases",
default=None,
help="Use another phasefile to override",
)
p.add_option("--comment", default="", help="Comments for this update")
opts, args = p.parse_args(args)
if len(args) != 2:
sys.exit(not p.print_help())
fastafile, sbtfile = args
pf = fastafile.rsplit(".", 1)[0]
idsfile = pf + ".ids"
phasefile = pf + ".phases"
namesfile = pf + ".names"
ids([fastafile, "--outfile={0}".format(idsfile)])
asndir = "asn.1"
mkdir(asndir)
entrez([idsfile, "--format=asn.1", "--outdir={0}".format(asndir)])
asn(glob("{0}/*".format(asndir)) + ["--outfile={0}".format(namesfile)])
if opts.phases is None:
gbdir = "gb"
mkdir(gbdir)
entrez([idsfile, "--format=gb", "--outdir={0}".format(gbdir)])
phase(
glob("{0}/*".format(gbdir)) + ["--outfile={0}".format(phasefile)])
else:
phasefile = opts.phases
assert op.exists(namesfile) and op.exists(phasefile)
newphasefile = phasefile + ".new"
newphasefw = open(newphasefile, "w")
comment = opts.comment
fastadir = "fasta"
sqndir = "sqn"
mkdir(fastadir)
mkdir(sqndir)
from jcvi.graphics.histogram import stem_leaf_plot
names = DictFile(namesfile)
assert len(set(names.keys())) == len(set(names.values()))
phases = DictFile(phasefile)
ph = [int(x) for x in phases.values()]
# vmin 1, vmax 4, bins 3
stem_leaf_plot(ph, 1, 4, 3, title="Counts of phases before updates")
logging.debug("Information loaded for {0} records.".format(len(phases)))
assert len(names) == len(phases)
newph = []
cmd = "faSplit byname {0} {1}/".format(fastafile, fastadir)
sh(cmd, outfile="/dev/null", errfile="/dev/null")
acmd = "tbl2asn -a z -p fasta -r {sqndir}"
acmd += " -i {splitfile} -t {sbtfile} -C tigr"
acmd += ' -j "{qualifiers}"'
acmd += " -A {accession_nv} -o {sqndir}/{accession_nv}.sqn -V Vbr"
acmd += ' -y "{comment}" -W T -T T'
qq = "[tech=htgs {phase}] [organism=Medicago truncatula] [strain=A17]"
nupdated = 0
for row in open(phasefile):
atoms = row.rstrip().split("\t")
# see formats.agp.phase() for column contents
accession, phase, clone = atoms[0], atoms[1], atoms[-1]
fafile = op.join(fastadir, accession + ".fa")
accession_nv = accession.split(".", 1)[0]
newid = names[accession_nv]
newidopt = "--newid={0}".format(newid)
cloneopt = "--clone={0}".format(clone)
splitfile, gaps = sequin([fafile, newidopt, cloneopt])
splitfile = op.basename(splitfile)
phase = int(phase)
assert phase in (1, 2, 3)
oldphase = phase
if gaps == 0 and phase != 3:
phase = 3
if gaps != 0 and phase == 3:
phase = 2
print("{0}\t{1}\t{2}".format(accession_nv, oldphase, phase),
file=newphasefw)
newph.append(phase)
qualifiers = qq.format(phase=phase)
if ";" in clone:
qualifiers += " [keyword=HTGS_POOLED_MULTICLONE]"
cmd = acmd.format(
accession=accession,
accession_nv=accession_nv,
sqndir=sqndir,
sbtfile=sbtfile,
splitfile=splitfile,
qualifiers=qualifiers,
comment=comment,
)
sh(cmd)
verify_sqn(sqndir, accession)
nupdated += 1
stem_leaf_plot(newph, 1, 4, 3, title="Counts of phases after updates")
print("A total of {0} records updated.".format(nupdated), file=sys.stderr)
def htgnew(args):
"""
%prog htgnew fastafile phasefile template.sbt
Prepare sqnfiles for submitting new Genbank HTG records.
`fastafile` contains the sequences.
`phasefile` contains the phase information, it is a two column file:
mth2-45h12 3
`template.sbt` is the Genbank submission template.
This function is simpler than htg, since the record names have not be
assigned yet (so less bookkeeping).
"""
from jcvi.formats.fasta import sequin
p = OptionParser(htgnew.__doc__)
p.add_option("--comment", default="",
help="Comments for this submission [default: %default]")
opts, args = p.parse_args(args)
if len(args) != 3:
sys.exit(not p.print_help())
fastafile, phasefile, sbtfile = args
comment = opts.comment
fastadir = "fasta"
sqndir = "sqn"
mkdir(fastadir)
mkdir(sqndir)
cmd = "faSplit byname {0} {1}/".format(fastafile, fastadir)
sh(cmd, outfile="/dev/null", errfile="/dev/null")
acmd = 'tbl2asn -a z -p fasta -r {sqndir}'
acmd += ' -i {splitfile} -t {sbtfile} -C tigr'
acmd += ' -j "[tech=htgs {phase}] [organism=Medicago truncatula] [strain=A17]"'
acmd += ' -o {sqndir}/{accession_nv}.sqn -V Vbr'
acmd += ' -y "{comment}" -W T -T T'
nupdated = 0
for row in open(phasefile):
name, phase = row.split()[:2]
fafile = op.join(fastadir, name + ".fa")
cloneopt = "--clone={0}".format(name)
splitfile, gaps = sequin([fafile, cloneopt])
splitfile = op.basename(splitfile)
accession = accession_nv = name
phase = int(phase)
assert phase in (1, 2, 3)
cmd = acmd.format(accession_nv=accession_nv, sqndir=sqndir,
sbtfile=sbtfile, splitfile=splitfile, phase=phase,
comment=comment)
sh(cmd)
verify_sqn(sqndir, accession)
nupdated += 1
print("A total of {0} records updated.".format(nupdated), file=sys.stderr)
def htgnew(args):
"""
%prog htgnew fastafile phasefile template.sbt
Prepare sqnfiles for submitting new Genbank HTG records.
`fastafile` contains the sequences.
`phasefile` contains the phase information, it is a two column file:
mth2-45h12 3
`template.sbt` is the Genbank submission template.
This function is simpler than htg, since the record names have not be
assigned yet (so less bookkeeping).
"""
from jcvi.formats.fasta import sequin
p = OptionParser(htgnew.__doc__)
p.add_option("--comment", default="", help="Comments for this submission")
opts, args = p.parse_args(args)
if len(args) != 3:
sys.exit(not p.print_help())
fastafile, phasefile, sbtfile = args
comment = opts.comment
fastadir = "fasta"
sqndir = "sqn"
mkdir(fastadir)
mkdir(sqndir)
cmd = "faSplit byname {0} {1}/".format(fastafile, fastadir)
sh(cmd, outfile="/dev/null", errfile="/dev/null")
acmd = "tbl2asn -a z -p fasta -r {sqndir}"
acmd += " -i {splitfile} -t {sbtfile} -C tigr"
acmd += ' -j "[tech=htgs {phase}] [organism=Medicago truncatula] [strain=A17]"'
acmd += " -o {sqndir}/{accession_nv}.sqn -V Vbr"
acmd += ' -y "{comment}" -W T -T T'
nupdated = 0
for row in open(phasefile):
name, phase = row.split()[:2]
fafile = op.join(fastadir, name + ".fa")
cloneopt = "--clone={0}".format(name)
splitfile, gaps = sequin([fafile, cloneopt])
splitfile = op.basename(splitfile)
accession = accession_nv = name
phase = int(phase)
assert phase in (1, 2, 3)
cmd = acmd.format(
accession_nv=accession_nv,
sqndir=sqndir,
sbtfile=sbtfile,
splitfile=splitfile,
phase=phase,
comment=comment,
)
sh(cmd)
verify_sqn(sqndir, accession)
nupdated += 1
print("A total of {0} records updated.".format(nupdated), file=sys.stderr)