def init_adendR(self):
adendR = self.adendR
h.pt3dclear(sec=adendR)
h.pt3dadd(-59, 0, 0, 1, sec=adendR)
h.pt3dadd(-104, -44, 0, 1, sec=adendR)
adendR.L = 350
adendR.diam = 1
adendR.nseg = 5
adendR.Ra = 200
adendR.cm = 2.4
adendR.insert('sAHP')
adendR.gsAHPbar_sAHP = 0
adendR.insert('nap')
adendR.gbar_nap = 0
adendR.insert('na3')
adendR.gbar_na3 = 0.015
adendR.ar2_na3 = 1
adendR.insert('leak')
adendR.glbar_leak = 0.031735
adendR.el_leak = -72
adendR.insert('kdr')
adendR.gbar_kdr = 0.002
adendR.insert('kap')
adendR.gkabar_kap = 0.002
adendR.insert('im')
adendR.gbar_im = 0
adendR.insert('hd')
adendR.ghdbar_hd = 1.5e-005
adendR.insert('capool')
adendR.taucas_capool = 1000
adendR.cainf_capool = 5e-005
adendR.fcas_capool = 0.05
adendR.insert('cadyn')
adendR.gcabar_cadyn = 0.00055
def init_p_dend(self):
p_dend = self.p_dend
h.pt3dclear(sec=p_dend)
h.pt3dadd(15, 0, 0, 1, sec=p_dend)
h.pt3dadd(75, 0, 0, 1, sec=p_dend)
p_dend.L = 400
p_dend.diam = 5
p_dend.nseg = 7
p_dend.Ra = 200
p_dend.cm = 2.4
p_dend.insert('cadyn')
p_dend.gcabar_cadyn = 0.00055
p_dend.insert('capool')
p_dend.taucas_capool = 1000
p_dend.cainf_capool = 5e-005
p_dend.fcas_capool = 0.05
p_dend.insert('hd')
p_dend.ghdbar_hd = 1.5e-005
p_dend.insert('im')
p_dend.gbar_im = 0
p_dend.insert('kap')
p_dend.gkabar_kap = 0
p_dend.insert('kdr')
p_dend.gbar_kdr = 0.002
p_dend.insert('leak')
p_dend.glbar_leak = 0.031735
p_dend.el_leak = -72
p_dend.insert('na3')
p_dend.gbar_na3 = 0.015
p_dend.ar2_na3 = 1
p_dend.insert('nap')
p_dend.gbar_nap = 0
p_dend.insert('sAHP')
p_dend.gsAHPbar_sAHP = 0
def init_atrunk(self):
atrunk = self.atrunk
h.pt3dclear(sec=atrunk)
h.pt3dadd(0, 0, 0, 1, sec=atrunk)
h.pt3dadd(-59, 0, 0, 1, sec=atrunk)
atrunk.L = 50
atrunk.diam = 2
atrunk.nseg = 3
atrunk.Ra = 200
atrunk.cm = 2.4
atrunk.insert('cadyn')
atrunk.gcabar_cadyn = 0.00055
atrunk.insert('leak')
atrunk.glbar_leak = 0.031735
atrunk.el_leak = -72
atrunk.insert('hd')
atrunk.ghdbar_hd = 1.5e-005
atrunk.insert('im')
atrunk.gbar_im = 0.00253
atrunk.insert('na3')
atrunk.gbar_na3 = 0.015
atrunk.ar2_na3 = 1
atrunk.insert('nap')
atrunk.gbar_nap = 0.000575
atrunk.insert('kdr')
atrunk.gbar_kdr = 0.002
atrunk.insert('kap')
atrunk.gkabar_kap = 0.002
atrunk.insert('capool')
atrunk.taucas_capool = 1000
atrunk.cainf_capool = 5e-005
atrunk.fcas_capool = 0.05
atrunk.insert('sAHP')
atrunk.gsAHPbar_sAHP = 0
def graph(self):
#Function: graph
#Input: self
#Process: create dendrite branch network
#Output: neuron
self.soma = h.Section(name='soma', cell=self)
self.dend = h.Section(name='dend', cell=self)
self.dend.connect(self.soma(1))
self.dend.diam = 2
self.dend.nseg = 10
self.soma.L = self.soma.diam = 5.0 # microns
h.pt3dclear(sec=self.soma)
h.pt3dadd(0, 0, 0, self.soma.diam, sec=self.soma)
h.pt3dadd(0 + self.soma.L,
0 + self.soma.L,
0 + self.soma.L,
self.soma.diam,
sec=self.soma)
h.pt3dadd(0 + self.soma.L,
0 + self.soma.L,
0 + self.soma.L,
self.soma.diam,
sec=self.soma)
h.pt3dadd(0 + self.soma.L + 10,
0 + self.soma.L + 10,
0 + self.soma.L + 10,
self.soma.diam,
sec=self.soma)
def draw(self):
""" Draw a visual representation of the optrode """
from neuron import gui
if not hasattr(self,'gOpt'):
from numpy import pi
self.gOpt=h.Shape(0)
self.gOpt.view(-2100, -2100, 4200, 4200, 230, 450, 200.64, 200.32)
self.gOpt.rotate(0, 0, 0, pi/2, 0, 0)
h.pt3dclear(sec = self.sec)
h.pt3dadd(self.x[0],
self.y[0],
self.z[0],
self.diameter,
sec = self.sec)
h.pt3dadd(self.x[1],
self.y[1],
self.z[1],
self.diameter,
sec = self.sec)
self.pOpt0 = h.IClamp(0,
sec = self.sec)
self.pOpt1 = h.IClamp(1,
sec = self.sec)
self.gOpt.point_mark(self.pOpt0, 1) # make start black
self.gOpt.point_mark(self.pOpt1, 3) # make output blue
self.gOpt.exec_menu("Show Diam")
self.gOpt.exec_menu("3D Rotate")
def init_soma(self):
soma = self.soma
h.pt3dclear(sec=soma)
h.pt3dadd(0, 0, 0, 1, sec=soma)
h.pt3dadd(15, 0, 0, 1, sec=soma)
soma.L = 25
soma.diam = 24.75
soma.nseg = 1
soma.Ra = 200
soma.cm = 2.4
soma.insert('cadyn')
soma.gcabar_cadyn = 0.00055
soma.insert('leak')
soma.glbar_leak = 0.031735
soma.el_leak = -72
soma.insert('hd')
soma.ghdbar_hd = 1.5e-005
soma.insert('na3')
soma.gbar_na3 = 0.045
soma.ar2_na3 = 1
soma.insert('nap')
soma.gbar_nap = 0.000575
soma.insert('kdr')
soma.gbar_kdr = 0.002
soma.insert('capool')
soma.taucas_capool = 1000
soma.cainf_capool = 5e-005
soma.fcas_capool = 0.05
soma.insert('sAHP')
soma.gsAHPbar_sAHP = 0.0012
soma.insert('im')
soma.gbar_im = 0.00253
soma.insert('kap')
soma.gkabar_kap = 0.002
def set_morphology(self):
total_area = 10000 # um2
self.soma.nseg = 1
self.soma.cm = 1 # uF/cm2
diam = sqrt(total_area) # um
L = diam / pi # um
h.pt3dclear(sec=self.soma)
h.pt3dadd(self.x, self.y, self.z, diam, sec=self.soma)
h.pt3dadd(self.x, self.y, self.z + L, diam, sec=self.soma)
def set_morphology(self): total_area = 10000 # um2 self.soma.nseg = 1 self.soma.cm = 1 # uF/cm2 diam = sqrt(total_area) # um L = diam/pi # um h.pt3dclear(sec=self.soma) h.pt3dadd(self.x, self.y, self.z, diam, sec=self.soma) h.pt3dadd(self.x, self.y, self.z+L, diam, sec=self.soma)
def make_sections(self):
SWC_types_inverse = {v:k for k,v in SWC_types.iteritems()}
# load the tree structure that represents the morphology
self.tree = btmorph.STree2()
self.tree.read_SWC_tree_from_file(self.swc_filename,types=range(10))
print('There are %d nodes in the full representation of the morphology.' % len(self.tree.get_nodes()))
# all the sections
self.sections = []
sections_map = {}
# describes how sections are connected
self.sections_connections = []
# a (temporary) list of all the sections that make up the apical dendrites
self.apical = []
# parse the tree
for node in self.tree:
if node is self.tree.root:
section = h.Section(name='{0}_{1}'.format(SWC_types_inverse[node.content['p3d'].type],node.index))
self.sections.append(section)
sections_map[node.index] = len(self.sections)-1
h.pt3dclear(sec=section)
self.soma.append(section)
elif len(node.children) == 1 and len(node.parent.children) > 1:
# the parent of the current node is a branching point: start a new section
section = h.Section(name='{0}_{1}'.format(SWC_types_inverse[node.content['p3d'].type],node.index))
self.sections.append(section)
self.sections_connections.append((len(self.sections)-1,sections_map[node.parent.index]))
sections_map[node.index] = len(self.sections)-1
h.pt3dclear(sec=section)
# assign it to the proper region
swc_type = node.content['p3d'].type
if swc_type == SWC_types['soma']:
self.soma.append(section)
h.distance(sec=soma[0])
elif swc_type == SWC_types['axon']:
self.axon.append(section)
elif swc_type == SWC_types['basal']:
self.basal.append(section)
elif swc_type == SWC_types['apical']:
self.apical.append(section)
else:
import pdb; pdb.set_trace()
else:
sections_map[node.index] = sections_map[node.parent.index]
section = self.sections[sections_map[node.parent.index]]
xyz = node.content['p3d'].xyz
h.pt3dadd(float(xyz[0]),float(xyz[1]),float(xyz[2]),2*float(node.content['p3d'].radius),sec=section)
# now that we have built all the sections we can subdivide those in the apical
# dendrite in proximal and distal
h.distance(sec=self.soma[0])
for sec in self.apical:
if h.distance(0.5, sec=sec) < self.parameters['proximal_limit']:
self.proximal.append(sec)
else:
self.distal.append(sec)
def basic_shape(self): self.soma.push() h.pt3dclear() h.pt3dadd(0, 0, 0, 1) h.pt3dadd(15, 0, 0, 1) h.pop_section() self.dend.push() h.pt3dclear() h.pt3dadd(15, 0, 0, 1) h.pt3dadd(105, 0, 0, 1) h.pop_section()
def init_dend(self):
dend = self.dend
h.pt3dclear(sec=dend)
h.pt3dadd(0, 0, 0, 1, sec=dend)
h.pt3dadd(-59, 0, 0, 1, sec=dend)
dend.L = 150
dend.diam = 10
dend.nseg = 1
dend.Ra = 150
dend.cm = 1
dend.insert('pas')
def shape_soma(self):
"""Define 3D shape of soma.
.. warning:: needed for gui representation of cell
DO NOT need to call h.define_shape() explicitly!
"""
h.pt3dclear(sec=self.soma)
# h.ptdadd(x, y, z, diam) -- if this function is run, clobbers
# self.soma.diam set above
h.pt3dadd(0, 0, 0, self.diam, sec=self.soma)
h.pt3dadd(0, self.L, 0, self.diam, sec=self.soma)
def basic_shape(self):
self.soma.push()
h.pt3dclear()
h.pt3dadd(0, 0, 0, 1)
h.pt3dadd(20, 0, 0, 1)
h.pop_section()
self.dend.push()
h.pt3dclear()
h.pt3dadd(15, 0, 0, 1)
h.pt3dadd(215, 0, 0, 1)
h.pop_section()
def set_geom(self, geom):
"""
Create 3d geometry of the neuron.
:param geom: List of 4d coordinates [x, y, z, diam].
:type geom: list
"""
self.push() # necessary to access Section in NEURON
h.pt3dclear()
for g in geom:
h.pt3dadd(g[0], g[1], g[2], g[3])
h.pop_section() # restore the previously accessed Section
def position(self):
'''
Adds 3D position
'''
i = 0
for sec in self.all:
h.pt3dclear()
h.pt3dadd(self.L*i, 0, self.zpozition, self.diam)
h.pt3dadd(self.L*(i+1), 0, self.zpozition, self.diam)
xyz = dict(x=self.L*(i+1), y=0, z=0)
self.coordinates.update({sec: xyz})
i+=1
def add_axon (self): # NB: paste-on axon if using SPI morphology (eg for comparing morph effect on dynamics) from PTAxonMorph import axonPts self.axon.append(h.Section(name="axon[0]")) self.all.append(self.axon[0]) self.axon[0].connect(self.soma[0], 0.0, 0.0) # clears 3d points h.pt3dclear() # define a logical connection point relative to the first 3-d point h.pt3dstyle(axonPts[0][0], axonPts[0][1], axonPts[0][2], axonPts[0][3], sec=self.axon[0]) # add axon points after first logical connection point for x, y, z, d in axonPts[1:]: h.pt3dadd(x, y, z, d, sec=self.axon[0])
def set_geom(self, geom):
"""
Create 3d geometry of the neuron.
:param geom: List of 4d coordinates [x, y, z, diam].
:type geom: list
"""
self.push() # necessary to access Section in NEURON
h.pt3dclear()
for g in geom:
h.pt3dadd(g[0], g[1], g[2], g[3])
h.pop_section() # restore the previously accessed Section
def position(self):
'''
Adds 3D position
'''
i = 0
for sec in self.node:
h.pt3dclear()
h.pt3dadd(self.interlength * i, 0, 0, self.nodeD)
h.pt3dadd((self.interlength * i + self.nodelength), 0, 0,
self.nodeD)
xyz = dict(x=(self.interlength * i + self.nodelength), y=0, z=0)
self.coordinates.update({sec: xyz})
i += 1
def shape_3D(self):
"""
Set the default shape of the cell in 3D coordinates.
Set soma(0) to the origin (0,0,0) and dend extending along
the X-axis.
"""
len1 = self.soma.L
h.pt3dclear(sec=self.soma)
h.pt3dadd(0, 0, 0, self.soma.diam, sec=self.soma)
h.pt3dadd(len1, 0, 0, self.soma.diam, sec=self.soma)
len2 = self.dend.L
h.pt3dclear(sec=self.dend)
h.pt3dadd(len1, 0, 0, self.dend.diam, sec=self.dend)
h.pt3dadd(len1 + len2, 0, 0, self.dend.diam, sec=self.dend)
def shape_3D(self):
"""
Set the default shape of the cell in 3D coordinates.
Set soma(0) to the origin (0,0,0) and dend extending along
the X-axis.
"""
len1 = self.soma[0].L
h.pt3dclear(sec=self.soma[0])
h.pt3dadd(0, 0, 0, self.soma[0].diam, sec=self.soma[0])
h.pt3dadd(len1, 0, 0, self.soma[0].diam, sec=self.soma[0])
len2 = self.dend[0].L
h.pt3dclear(sec=self.dend[0])
h.pt3dadd(len1, 0, 0, self.dend[0].diam, sec=self.dend[0])
h.pt3dadd(len1 + len2, 0, 0, self.dend[0].diam, sec=self.dend[0])
def _create_sections(self, sections, topology):
"""Create soma and set geometry.
Notes
-----
By default neuron uses xy plane
for height and xz plane for depth. This is opposite for model as a
whole, but convention is followed in this function ease use of gui.
"""
# shift cell to self.pos and reorient apical dendrite
# along z direction of self.pos
dx = self.pos[0] - self.sections['soma'].end_pts[0][0]
dy = self.pos[1] - self.sections['soma'].end_pts[0][1]
dz = self.pos[2] - self.sections['soma'].end_pts[0][2]
for sec_name in sections:
sec = h.Section(name=f'{self.name}_{sec_name}')
self._nrn_sections[sec_name] = sec
h.pt3dclear(sec=sec)
h.pt3dconst(0, sec=sec) # be explicit, see documentation
for pt in sections[sec_name].end_pts:
h.pt3dadd(pt[0] + dx, pt[1] + dy, pt[2] + dz, 1, sec=sec)
# with pt3dconst==0, these will alter the 3d points defined above!
sec.L = sections[sec_name].L
sec.diam = sections[sec_name].diam
sec.Ra = sections[sec_name].Ra
sec.cm = sections[sec_name].cm
if sec.L > 100.: # 100 um
sec.nseg = int(sec.L / 50.)
# make dend.nseg odd for all sections
if not sec.nseg % 2:
sec.nseg += 1
if topology is None:
topology = list()
# Connects sections of THIS cell together.
for connection in topology:
parent_sec = self._nrn_sections[connection[0]]
child_sec = self._nrn_sections[connection[2]]
parent_loc = connection[1]
child_loc = connection[3]
child_sec.connect(parent_sec, parent_loc, child_loc)
# be explicit about letting sec.L dominate over the 3d points used by
# h.pt3dadd(); see
# https://nrn.readthedocs.io/en/latest/python/modelspec/programmatic/topology/geometry.html?highlight=pt3dadd#pt3dadd # noqa
h.define_shape()
def set_geometry(self, p_dend):
"""Define shape of the neuron and connect sections.
Parameters
----------
p_dend : dict | None
Nested dictionary. The outer dictionary has keys
with names of dendrites and the inner dictionary
specifies the geometry of these sections.
* L: length of a section in microns
* diam: diameter of a section in microns
* cm: membrane capacitance in micro-Farads
* Ra: axial resistivity in ohm-cm
"""
sec_pts, sec_lens, sec_diams, _, topology = self.secs()
# Connects sections of THIS cell together.
for connection in topology:
# XXX: risky to use self.soma as default. Unfortunately there isn't
# a dictionary with all the sections (including soma)
parent_sec = self.dends.get(connection[0], self.soma)
parent_loc = connection[1]
child_sec = self.dends.get(connection[2], self.soma)
child_loc = connection[3]
child_sec.connect(parent_sec, parent_loc, child_loc)
# Neuron shape based on Jones et al., 2009
for sec in [self.soma] + self.list_dend:
h.pt3dclear(sec=sec)
sec_name = sec.name().split('_', 1)[1]
for pt in sec_pts[sec_name]:
h.pt3dadd(pt[0], pt[1], pt[2], 1, sec=sec)
sec.L = sec_lens[sec_name]
sec.diam = sec_diams[sec_name]
# resets length,diam,etc. based on param specification
for key in p_dend:
# set dend props
self.dends[key].L = p_dend[key]['L']
self.dends[key].diam = p_dend[key]['diam']
self.dends[key].Ra = p_dend[key]['Ra']
self.dends[key].cm = p_dend[key]['cm']
# set dend nseg
if p_dend[key]['L'] > 100.:
self.dends[key].nseg = int(p_dend[key]['L'] / 50.)
# make dend.nseg odd for all sections
if not self.dends[key].nseg % 2:
self.dends[key].nseg += 1
def add_axon(self):
self.axon.append(h.Section(name="axon[0]"))
self.all.append(self.axon[0])
self.axon[0].connect(self.soma[0], 0.0, 0.0)
# clears 3d points
h.pt3dclear()
# define a logical connection point relative to the first 3-d point
h.pt3dstyle(axonPts[0][0],
axonPts[0][1],
axonPts[0][2],
axonPts[0][3],
sec=self.axon[0])
# add axon points after first logical connection point
for x, y, z, d in axonPts[1:]:
h.pt3dadd(x, y, z, d, sec=self.axon[0])
def fix_axon_peri_v2(hobj):
"""Replace reconstructed axon with a stub (keep order); BBP version
:param hobj: hoc object
"""
if hasattr(hobj, 'axon'):
for i,sec in enumerate(hobj.axon):
h.pt3dclear(sec=sec)
if i < 2:
sec.L = 30
sec.diam = 1
else:
sec.L = 1e-6
sec.diam = 1
h.define_shape()
def position(self, axon, last_x, plus_y):
'''
Adds 3D position
'''
i = 0
for sec in axon.node:
h.pt3dclear()
h.pt3dadd((last_x + axon.interlength * i), i * plus_y, 0,
axon.nodeD)
h.pt3dadd((last_x + axon.interlength * i + axon.nodelength),
i * 2 * plus_y, 0, axon.nodeD)
xyz = dict(x=(last_x + axon.interlength * i + axon.nodelength),
y=i * 2 * plus_y,
z=0)
self.coordinates.update({sec: xyz})
i += 1
def init_soma(self):
soma = self.soma
h.pt3dclear(sec=soma)
h.pt3dadd(0, 0, 0, 1, sec=soma)
h.pt3dadd(15, 0, 0, 1, sec=soma)
soma.L = 50
soma.diam = 50
soma.nseg = 1
soma.Ra = 150
soma.cm = 1
soma.insert('leak')
soma.glbar_leak = 1 / 3333.33
soma.el_leak = -70
soma.insert('kdr')
soma.gkdrbar_kdr = 0.036
soma.insert('na')
soma.gnabar_na = 0.12
def test_transfer_resistance():
"""Test transfer resistances calculated correctly"""
from neuron import h
from hnn_core.extracellular import _transfer_resistance
sec = h.Section(name='dend')
h.pt3dclear(sec=sec)
h.pt3dadd(0, 0, 0, 1, sec=sec)
h.pt3dadd(0, 1, 0, 1, sec=sec) # section oriented along y-axis
sec.L = 300
sec.diam = 8
sec.nseg = 5
# NB segment lengths aren't equal! First/last segment center point is
# closer to respective end point than to next/previous segment!
seg_ctr_pts = [0]
seg_ctr_pts.extend([seg.x * sec.L for seg in sec])
seg_ctr_pts.append(sec.L)
seg_lens = np.diff(seg_ctr_pts)
first_len = seg_lens[0]
seg_lens = np.array([first_len] + list(seg_lens[2:]))
seg_ctr_pts = seg_ctr_pts[1:-1] # remove end points again
conductivity = 0.3
elec_pos = (10, 150, 0)
target_vals = {'psa': list(), 'lsa': list()}
for seg_idx in range(sec.nseg):
# PSA: distance to middle segment == electrode x-position
var_r_psa = np.sqrt(elec_pos[0] ** 2 +
(elec_pos[1] - seg_ctr_pts[seg_idx]) ** 2)
target_vals['psa'].append(
1000 / (4. * np.pi * conductivity * var_r_psa))
# LSA: calculate L and H variables relative to segment endpoints
var_l = elec_pos[1] - (seg_ctr_pts[seg_idx] - seg_lens[seg_idx])
var_h = elec_pos[1] - (seg_ctr_pts[seg_idx] + seg_lens[seg_idx])
var_r_lsa = elec_pos[0] # just use the axial distance
target_vals['lsa'].append(
1000 * np.log(np.abs(
(np.sqrt(var_h ** 2 + var_r_lsa ** 2) - var_h) /
(np.sqrt(var_l ** 2 + var_r_lsa ** 2) - var_l)
)) / (4. * np.pi * conductivity * 2 * seg_lens[seg_idx]))
for method in ['psa', 'lsa']:
res = _transfer_resistance(sec, elec_pos, conductivity, method)
assert_allclose(res, target_vals[method], rtol=1e-12, atol=0.)
def __init__(self):
self.soma = h.Section(name="soma")
h.pt3dclear()
h.pt3dadd(0, 0, 0, 1)
h.pt3dadd(15, 0, 0, 1)
self.x = self.y = self.z = 0
self.soma.L = 10
self.soma.diam = 3.1831
self.soma.Ra = 100
self.soma.cm = 1
self.soma.insert('pas')
self.soma.g_pas = 5e-5
self.soma.e_pas = -70
self.izh = h.Izh(0.5, sec=self.soma)
self.izh.amp = 0
self.all = h.SectionList()
self.all.append()
self.make_synapses()
def shape_3D(self):
"""
This method defines the 3D shape of each SimpleCell object, so
that it can later be drawn on a plot. It makes use of the NEURON
pt3dadd() function defined for cellular compartment objects.
"""
somalen = self.soma.L
dendlen = self.dend.L
h.pt3dclear(sec=self.soma)
h.pt3dadd(0, 0, 0, self.soma.diam, sec=self.soma)
h.pt3dadd(somalen, 0, 0, self.soma.diam, sec=self.soma)
h.pt3dclear(sec=self.dend)
h.pt3dadd(somalen, 0, 0, self.dend.diam, sec=self.dend)
h.pt3dadd(somalen + dendlen, 0, 0, self.dend.diam, sec=self.dend)
self.all = h.SectionList()
self.all.wholetree(sec=self.soma)
def set_position(self, x, y, z):
""" Move electrode to new coordinates:
x0, y0, z0: optrode input
x1, x1, z1: optrode output
"""
from numpy.linalg import norm
self._x=x
self._y=y
self._z=z
xyz0,xyz1=self.xyz
#self.sec.L=dist(xyz0,xyz1)
self.sec.L = norm(xyz1-xyz0)
h.pt3dclear(sec=self.sec)
h.pt3dadd(float(x[0]), float(y[0]), float(z[0]),
self.radius * 2, sec=self.sec)
h.pt3dadd(float(x[1]), float(y[1]), float(z[1]),
self.radius * 2, sec=self.sec)
self.calc_tx()
if hasattr(self, "gOpt"): self.draw()
if hasattr(self, "mlab_tube"): self.display()
def position(self):
'''
Adds 3D position
'''
if self.numofmodel == 11 or self.numofmodel == 12:
h.pt3dclear()
h.pt3dadd(0, 0, self.zpozition, self.diam)
h.pt3dadd(self.L, 0, self.zpozition, self.diam)
xyz = dict(x=self.L, y=0, z=0)
self.coordinates.update({self.branch: xyz})
for i in range(70):
h.pt3dclear()
h.pt3dadd(self.L * (i + 1), 0, self.zpozition, self.diam)
h.pt3dadd(self.L * (i + 2), 0, self.zpozition, self.diam)
xyz = dict(x=self.L * (i + 2), y=0, z=0)
self.coordinates.update({self.stimsec[i]: xyz})
for i in range(70, 120):
h.pt3dclear()
h.pt3dadd(self.L * (i + 1), (i - 70) * 8, self.zpozition,
self.diam)
h.pt3dadd(self.L * (i + 2), (i - 69) * 8, self.zpozition,
self.diam)
xyz = dict(x=self.L * (i + 2), y=(i - 69) * 8, z=0)
self.coordinates.update({self.stimsec[i]: xyz})
for i in range(120, 170):
h.pt3dclear()
h.pt3dadd(self.L * (i - 49), (i - 120) * (-8), self.zpozition,
self.diam)
h.pt3dadd(self.L * (i - 48), (i - 119) * (-8), self.zpozition,
self.diam)
xyz = dict(x=self.L * (i - 48), y=(i - 119) * (-8), z=0)
self.coordinates.update({self.stimsec[i]: xyz})
else:
i = 0
for sec in self.all:
h.pt3dclear()
h.pt3dadd(self.L * i, 0, self.zpozition, self.diam)
h.pt3dadd(self.L * (i + 1), 0, self.zpozition, self.diam)
xyz = dict(x=self.L * (i + 1), y=0, z=0)
self.coordinates.update({sec: xyz})
i += 1
def shape_3D(self):
"""
Set the default shape of the cell in 3D coordinates.
Set soma(0) to the origin (0,0,0) and dend extending along
the X-axis. -Innacurate
"""
len1 = self.soma.L
h.pt3dclear(sec=self.soma)
h.pt3dadd(0, 0, 0, self.soma.diam, sec=self.soma)
h.pt3dadd(0, len1, 0, self.soma.diam, sec=self.soma)
len2 = self.dendL.L
h.pt3dclear(sec=self.dendL)
h.pt3dadd(0, len1, 0, self.dendL.diam, sec=self.dendL)
h.pt3dadd(-len2 * cos(pi / 4),
len2 * sin(pi / 4),
0,
self.dendL.diam,
sec=self.dendL)
h.pt3dclear(sec=self.dendR)
h.pt3dadd(0, len1, 0, self.dendR.diam, sec=self.dendR)
h.pt3dadd(len2 * cos(pi / 4),
len2 * sin(pi / 4),
0,
self.dendR.diam,
sec=self.dendR)
def basic_shape(self):
h.pt3dclear(sec=self.soma)
h.pt3dadd(0, 0, 0, 1, sec=self.soma)
h.pt3dadd(15, 0, 0, 1, sec=self.soma)
h.pt3dclear(sec=self.dend1)
h.pt3dadd(15, 0, 0, 1, sec=self.dend1)
h.pt3dadd(90, 0, 0, 1, sec=self.dend1)
h.pt3dclear(sec=self.dend2)
h.pt3dadd(0, 0, 0, 1, sec=self.dend2)
h.pt3dadd(-74, 0, 0, 1, sec=self.dend2)
h.pt3dclear(sec=self.axon)
h.pt3dadd(15, 0, 0, 1, sec=self.axon)
h.pt3dadd(15, 120, 0, 1, sec=self.axon)
def _create_sections(self, p_secs, topology):
"""Create soma and set geometry.
Notes
-----
By default neuron uses xy plane
for height and xz plane for depth. This is opposite for model as a
whole, but convention is followed in this function ease use of gui.
"""
for sec_name in p_secs:
sec = h.Section(name=f'{self.name}_{sec_name}')
self.sections[sec_name] = sec
h.pt3dclear(sec=sec)
for pt in p_secs[sec_name]['sec_pts']:
h.pt3dadd(pt[0], pt[1], pt[2], 1, sec=sec)
sec.L = p_secs[sec_name]['L']
sec.diam = p_secs[sec_name]['diam']
sec.Ra = p_secs[sec_name]['Ra']
sec.cm = p_secs[sec_name]['cm']
if sec.L > 100.: # 100 um
sec.nseg = int(sec.L / 50.)
# make dend.nseg odd for all sections
if not sec.nseg % 2:
sec.nseg += 1
if topology is None:
topology = list()
# Connects sections of THIS cell together.
for connection in topology:
parent_sec = self.sections[connection[0]]
child_sec = self.sections[connection[2]]
parent_loc = connection[1]
child_loc = connection[3]
child_sec.connect(parent_sec, parent_loc, child_loc)
def update_coords_and_radii(self, blender_section):
self.nseg = blender_section["nseg"]
self.point_count = blender_section["point_count"]
self.coords = blender_section["coords"]
self.radii = blender_section["radii"]
nrn_section = self.nrn_section
# Use 3D points as the L and diam sources
h.pt3dconst(1, sec=nrn_section)
# Clear the existing points - and allocate room for the incoming points
h.pt3dclear(self.point_count, sec=nrn_section)
# Use vectorization to add the points to section
coords = np.array(self.coords).reshape((-1, 3))
diams = np.array(self.radii) * 2.0
xvec = h.Vector(coords[:, 0])
yvec = h.Vector(coords[:, 1])
zvec = h.Vector(coords[:, 2])
dvec = h.Vector(diams)
h.pt3dadd(xvec, yvec, zvec, dvec, sec=nrn_section)
def basic_shape(self):
self.axon.push()
h.pt3dclear()
h.pt3dadd(0, 0, 0, 1)
h.pt3dadd(80, 0, 0, 1)
h.pop_section()
self.soma.push()
h.pt3dclear()
h.pt3dadd(80, 0, 0, 20)
h.pt3dadd(100, 0, 0, 20)
h.pop_section()
self.dend.push()
h.pt3dclear()
h.pt3dadd(100, 0, 0, 2)
h.pt3dadd(900, 0, 0, 2)
h.pop_section()
def define_geometry(self):
self.soma.nseg = 1
self.soma.L = 38.42
self.soma.diam = 26
h.pt3dclear(sec=self.soma)
h.pt3dadd(0, 0, 0, 26, sec=self.soma)
h.pt3dadd(0, 38.42, 0, 26, sec=self.soma)
h.define_shape()
self.dend1[0].nseg = 1
h.pt3dclear(sec=self.dend1[0])
h.pt3dadd(0, 38.42, 0, 10.28, sec=self.dend1[0])
h.pt3dadd(0, 50.91, 0, 10.28, sec=self.dend1[0])
h.define_shape()
self.dend1[1].nseg = 1
h.pt3dclear(sec=self.dend1[1])
h.pt3dadd(0, 50.91, 0, 8.5, sec=self.dend1[1])
h.pt3dadd(0, 135.58, 0, 8.5, sec=self.dend1[1])
h.define_shape()
def __init__(self,position, record_all = 0, sigma_L = 0, gid = 0, lkg2_noise=0, lkg2_gbar=6e-5):
self.record_all = record_all
if record_all:
print "Recording all in Grc"
self.soma = h.Section(cell=self)
self.soma.nseg = 1
L = 9.76 #um
#(9.76e-6)**2 * 3.14159265359 * 1e-6 / (1e-2)**2 = 3 pF
self.gid = gid
if sigma_L == 0:
self.soma.L = L
self.soma.diam = L
else:
np.random.seed(self.gid*40)
self.soma.L = np.random.normal(L, L*sigma_L, 1).clip(min=L*sigma_L)
self.soma.diam = self.soma.L
print "soma.L:", self.soma.L
self.soma.cm = 1
self.soma.Ra = 100
# h.celsius = 37
self.lkg2_noise = lkg2_noise
self.whatami = "grc"
self.soma.push()
h.pt3dclear()
h.pt3dadd(position.item(0), position.item(1) - self.soma.L, position.item(2), self.soma.diam)
h.pt3dadd(position.item(0), position.item(1) + self.soma.L, position.item(2), self.soma.diam)
h.pop_section()
self.record = {}
self.record['L'] = h.Vector(np.array([self.soma.L]))
self.record['position'] = h.Vector(position)
self.soma.insert('GRANULE_LKG1')
if lkg2_noise > 0:
print "noise"
self.noise = h.Random() # provides NOISE with random stream
self.fluct = h.GRANULE_LKG2_noise(self.soma(0.5))
self.fluct.gbar = lkg2_gbar
self.fluct.std_i = lkg2_noise
self.fluct.tau_i = 100
self.fluct.noiseFromRandom(self.noise) # connect random generator!
self.noise.MCellRan4(1, gid+1) # set lowindex to gid+1, set highindex to > 0
self.noise.normal(0,1)
else:
self.soma.insert('GRANULE_LKG2')
#self.soma(0.5).GRANULE_LKG2.gbar = 6e-5
self.soma.insert('GRANULE_Nmda_leak')
self.soma.insert('GRANULE_NA')
self.soma.insert('GRANULE_NAR')
self.soma.insert('GRANULE_PNA')
self.soma.insert('GRANULE_KV')
self.soma.insert('GRANULE_KA')
self.soma.insert('GRANULE_KIR')
self.soma.insert('GRANULE_KCA')
self.soma.insert('GRANULE_KM')
self.soma.insert('GRANULE_CA')
self.soma.insert('GRANULE_CALC')
h.usetable_GRANULE_NA = 1
h.usetable_GRANULE_NAR = 1
h.usetable_GRANULE_PNA = 1
h.usetable_GRANULE_KV = 1
h.usetable_GRANULE_KA = 1
h.usetable_GRANULE_KIR = 1
h.usetable_GRANULE_KCA = 0
h.usetable_GRANULE_KM = 1
h.usetable_GRANULE_CA = 1
self.soma.ena = 87.39
self.soma.ek = -84.69
self.soma.eca = 129.33
self.MF_L = []
self.GOC_L = []
self.mfncpc = []
self.gocncpc = []
self.spike = h.NetStim(0.5, sec= self.soma)
self.spike.start = -10
self.spike.number = 1
self.spike.interval = 1e9
self.nc_spike = h.NetCon(self.soma(1)._ref_v, self.spike,-20,1,1, sec = self.soma)
self.record['spk'] = h.Vector()
self.nc_spike.record(self.record['spk'])
if self.record_all:
self.record['vm'] = h.Vector()
self.record['vm'].record(self.soma(.5)._ref_v, sec = self.soma)
self.record['time'] = h.Vector()
self.record['time'].record(h._ref_t)
self.nc_syn = []
def load_morphology(self):
# load the tree structure that represents the morphology
self.tree = btmorph.STree2()
self.tree.read_SWC_tree_from_file(self.swc_filename,types=range(10))
if self.min_distance > 0.:
# simplify the morphology
total = len(self.tree.get_nodes())
removed = []
sys.stdout.write('Simplifying the morphology... ')
sys.stdout.flush()
simplify_tree(self.tree.root, self.min_distance, (SWC_types['soma'],SWC_types['axon']), removed)
sys.stdout.write('removed %d nodes out of %d.\n' % (len(removed),total))
self.simplified_swc_filename = '.'.join(self.swc_filename.split('.')[:-1]) + \
'_simplified_%g_um.swc' % self.min_distance
self.tree.write_SWC_tree_to_file(self.simplified_swc_filename)
# all the sections, indexed by the corresponding index in the SWC file
self.sections = {}
# a list of all the sections that make up the soma
self.soma = []
# a list of all the sections that make up the axon
self.axon = []
# a list of all the sections that make up the basal dendrites
self.basal = []
# a list of all the sections that make up the apical dendrites
self.apical = []
# parse the tree!
for node in self.tree:
if node is self.tree.root:
continue
section = h.Section(name='sec_{0}'.format(node.index))
swc_type = node.content['p3d'].type
if swc_type == SWC_types['soma']:
section.cm = self.Cm['soma']
section.Ra = self.Ra['soma']
self.soma.append(section)
elif swc_type == SWC_types['axon']:
section.cm = self.Cm['axon']
section.Ra = self.Ra['axon']
self.axon.append(section)
elif swc_type == SWC_types['basal']:
section.cm = self.Cm['dend']
section.Ra = self.Ra['dend']
self.basal.append(section)
elif swc_type == SWC_types['apical']:
section.cm = self.Cm['dend']
section.Ra = self.Ra['dend']
self.apical.append(section)
if not node.parent is None:
pPos = node.parent.content['p3d']
cPos = node.content['p3d']
c_xyz = cPos.xyz
p_xyz = pPos.xyz
h.pt3dclear(sec=section)
h.pt3dadd(float(p_xyz[0]),float(p_xyz[1]),float(p_xyz[2]),float(pPos.radius),sec=section)
h.pt3dadd(float(c_xyz[0]),float(c_xyz[1]),float(c_xyz[2]),float(cPos.radius),sec=section)
# nseg according to NEURON book; too high in general...
section.nseg = int((section.L/(0.1*h.lambda_f(100))+0.9)/2)*2 + 1
try:
section.connect(self.sections[node.parent.index],1,0)
except:
if not section is self.soma[0]:
section.connect(self.soma[0],1,0)
self.sections[node.index] = section
def createNEURONObj(self, prop):
# set params for all sections
for sectName,sectParams in prop['sections'].iteritems():
# create section
if sectName not in self.secs:
self.secs[sectName] = {} # create sect dict if doesn't exist
self.secs[sectName]['hSection'] = h.Section(name=sectName) # create h Section object
sec = self.secs[sectName] # pointer to section
# add distributed mechanisms
if 'mechs' in sectParams:
for mechName,mechParams in sectParams['mechs'].iteritems():
if mechName not in sec['mechs']:
sec['mechs'][mechName] = {}
sec['hSection'].insert(mechName)
for mechParamName,mechParamValue in mechParams.iteritems(): # add params of the mechanism
mechParamValueFinal = mechParamValue
for iseg,seg in enumerate(sec['hSection']): # set mech params for each segment
if type(mechParamValue) in [list]:
mechParamValueFinal = mechParamValue[iseg]
seg.__getattribute__(mechName).__setattr__(mechParamName,mechParamValueFinal)
# add point processes
if 'pointps' in sectParams:
for pointpName,pointpParams in sectParams['pointps'].iteritems():
#if self.tags['cellModel'] == pointpParams: # only required if want to allow setting various cell models in same rule
if pointpName not in sec['pointps']:
sec['pointps'][pointpName] = {}
pointpObj = getattr(h, pointpParams['_type'])
loc = pointpParams['_loc'] if '_loc' in pointpParams else 0.5 # set location
sec['pointps'][pointpName]['hPointp'] = pointpObj(loc, sec = sec['hSection']) # create h Pointp object (eg. h.Izhi2007b)
for pointpParamName,pointpParamValue in pointpParams.iteritems(): # add params of the point process
if not pointpParamName.startswith('_'):
setattr(sec['pointps'][pointpName]['hPointp'], pointpParamName, pointpParamValue)
# add synapses
if 'syns' in sectParams:
for synName,synParams in sectParams['syns'].iteritems():
if synName not in sec['syns']:
sec['syns'][synName] = {}
synObj = getattr(h, synParams['_type'])
loc = synParams['_loc'] if '_loc' in synParams else 0.5 # set location
sec['syns'][synName]['hSyn'] = synObj(loc, sec = sec['hSection']) # create h Syn object (eg. h.Exp2Syn)
for synParamName,synParamValue in synParams.iteritems(): # add params of the synapse
if not synParamName.startswith('_'):
setattr(sec['syns'][synName]['hSyn'], synParamName, synParamValue)
# set geometry params
if 'geom' in sectParams:
for geomParamName,geomParamValue in sectParams['geom'].iteritems():
if not type(geomParamValue) in [list, dict]: # skip any list or dic params
setattr(sec['hSection'], geomParamName, geomParamValue)
# set 3d geometry
if 'pt3d' in sectParams['geom']:
h.pt3dclear(sec=sec['hSection'])
x = self.tags['x']
if 'ynorm' in self.tags and 'sizeY' in f.net.params:
y = self.tags['ynorm'] * f.net.params['sizeY']/1e3 # y as a func of ynorm and cortical thickness
else:
y = self.tags['y']
z = self.tags['z']
for pt3d in sectParams['geom']['pt3d']:
h.pt3dadd(x+pt3d[0], y+pt3d[1], z+pt3d[2], pt3d[3], sec=sec['hSection'])
# set topology
for sectName,sectParams in prop['sections'].iteritems(): # iterate sects again for topology (ensures all exist)
sec = self.secs[sectName] # pointer to section # pointer to child sec
if 'topol' in sectParams:
if sectParams['topol']:
sec['hSection'].connect(self.secs[sectParams['topol']['parentSec']]['hSection'], sectParams['topol']['parentX'], sectParams['topol']['childX']) # make topol connection
#
scale = 5
import numpy
for sec in h.allsec():
if 'soma' not in sec.name():
for i in xrange(int(h.n3d(sec=sec))):
d = h.diam3d(i, sec=sec)
h.pt3dchange(i, d * scale, sec=sec)
elif 'soma' in sec.name():
x, y, z, diam = [], [], [], []
for i in xrange(int(h.n3d(sec=sec))):
x.append(h.x3d(i, sec=sec))
y.append(h.y3d(i, sec=sec))
z.append(h.z3d(i, sec=sec))
diam.append(h.diam3d(i, sec=sec))
h.pt3dclear(sec=sec)
x, y, z, diam = scale * numpy.array(x), scale * numpy.array(y), scale * numpy.array(z), scale * numpy.array(diam)
i = int(len(x) / 2)
midptx, midpty, midptz = x[i], y[i], z[i]
x -= midptx / 2.
y -= midpty / 2.
z -= midptz / 2.
for xpt, ypt, zpt, diampt in zip(x, y, z, diam):
h.pt3dadd(xpt, ypt, zpt, diampt, sec=sec)
# begin with a full rotation for 40 frames
for i in xrange(40):
ps.rotate(0, 0, 0, 0, 6.283 / 40., 0)
h.doNotify()
def fillshape(s1, s2):
s2.push()
h.pt3dclear()
for x in s1.points:
h.pt3dadd(x[0], x[1], x[2], x[3])
h.pop_section()
def shape_3D(self): h.pt3dclear(sec = self.soma[0])
def __init__(self,position):
self.soma = h.Section(name='soma')
self.soma.nseg = 1
self.soma.diam = 9.76
self.soma.L = 9.76
self.soma.cm = 1
self.soma.Ra = 100
#Shouldn't change a global param like this in a class...
#h.celsius = 37
self.whatami = "grc"
self.soma.push()
h.pt3dclear()
h.pt3dadd(position.item(0), position.item(1) - self.soma.L, position.item(2), self.soma.diam)
h.pt3dadd(position.item(0), position.item(1) + self.soma.L, position.item(2), self.soma.diam)
h.pop_section()
self.soma.insert('GRANULE_LKG1')
self.soma.insert('GRANULE_LKG2')
self.soma.insert('GRANULE_Nmda_leak')
self.soma.insert('GRANULE_NA')
self.soma.insert('GRANULE_NAR')
self.soma.insert('GRANULE_PNA')
self.soma.insert('GRANULE_KV')
self.soma.insert('GRANULE_KA')
self.soma.insert('GRANULE_KIR')
self.soma.insert('GRANULE_KCA')
self.soma.insert('GRANULE_KM')
self.soma.insert('GRANULE_CA')
self.soma.insert('GRANULE_CALC')
h.usetable_GRANULE_NA = 1
h.usetable_GRANULE_NAR = 1
h.usetable_GRANULE_PNA = 1
h.usetable_GRANULE_KV = 1
h.usetable_GRANULE_KA = 1
h.usetable_GRANULE_KIR = 1
h.usetable_GRANULE_KCA = 0
h.usetable_GRANULE_KM = 1
h.usetable_GRANULE_CA = 1
self.soma.ena = 87.39
self.soma.ek = -84.69
self.soma.eca = 129.33
self.MF_L = []
self.GOC_L = []
self.mfncpc = []
self.gocncpc = []
self.spike = h.NetStim(0.5, sec= self.soma)
self.spike.start = -10
self.spike.number = 1
self.spike.interval = 1e9
self.nc = h.NetCon(self.soma(1)._ref_v, self.spike, sec = self.soma)
self.nc.threshold=-20
self.nc.delay=0
self.nc.weight[0]=1
self.SpikeTrain_output = [h.Vector(),h.Vector()]
self.nc.record(self.SpikeTrain_output[1], self.SpikeTrain_output[0], 1)
self.vm = h.Vector()
self.vm.record(self.soma(.5)._ref_v, sec = self.soma)
self.time = h.Vector()
self.time.record(h._ref_t)
