def test_labware_create(dummy_db):
from opentrons import labware
lw_name = '15-well-plate'
if lw_name in labware.list():
database.delete_container(lw_name)
n_cols = 5
n_rows = 3
col_space = 12
row_space = 18
diameter = 5
height = 20
volume = 3.14 * (diameter / 2.0)**2
res = labware.create(lw_name, (n_cols, n_rows), (col_space, row_space),
diameter, height, volume)
lw = database.load_container(lw_name)
database.delete_container(lw_name)
assert len(lw.wells()) is n_cols * n_rows
for well in lw.wells():
name = well.get_name()
assert res[name].coordinates() == well.coordinates()
for prop in ['height', 'diameter']:
assert res[name].properties[prop] == well.properties[prop]
assert lw.well("B5").coordinates() == ((n_cols - 1) * col_space, row_space,
0)
assert lw.well("C3").coordinates() == (2 * col_space, 0, 0)
assert lw.well(2).get_name() == 'C1'
#If false, ignores any labware that is already defined in robot
REPLACE = False
#define a dict of custom plates. Can add as many as necessary
custom_plates = dict()
custom_plates['CCL_ARTBot_canvas'] = dict(grid=(1, 1),
spacing=(0, 0),
diameter=60,
depth=13,
volume=100)
custom_plates['falcon_6_wellplate_15.5ml_flat'] = dict(grid=(3, 2),
spacing=(39.3, 39.3),
diameter=34.75,
depth=18,
volume=15500)
custom_plates['nunc_8_wellplate_flat'] = dict(grid=(4, 2),
spacing=(31, 40.5),
diameter=31,
depth=13.3,
volume=13000)
for plate_name in custom_plates:
if plate_name in labware.list():
if REPLACE:
database.delete_container(plate_name)
else:
continue
labware.create(plate_name, **custom_plates[plate_name])
'author': 'Ben Avants <*****@*****.**>',
'source': 'HydraBot Vivarium Project'
}
def circle(location, radius, steps=50, pause=.05):
step = 2 * 3.14159 / steps
for i in range(steps):
vect = location.from_center(r=radius, theta=step*i, h=1)
pipette.move_to((location, vect), strategy='direct')
sleep(pause)
pipette.move_to(location, strategy='direct')
redefine = False
if redefine is True:
from opentrons.data_storage import database
database.delete_container('3x2_plate')
database.delete_container('3x2_plate_eppendorf')
database.delete_container('3x2_plate_corning')
database.delete_container('3x2_plate_falcon')
# 6-well plate custom entry
plate_name = '3x2_plate_eppendorf'
if plate_name not in labware.list():
hydra_plate = labware.create(
plate_name,
grid=(3, 2),
spacing=(38, 40),
diameter=35,
depth=17,
volume=3000
)
from opentrons.data_storage import database
metadata = {
'protocolName' : 'Coating',
'description' : 'Coating of the samples',
'source' : 'https://github.com/Zildj1an/SELEX'
}
# [0] Our design for the three modules
# X,Y,Z,A speeds for lateral,front and vertical motion for left and right
# B,C plunger speed for motor
#max_speed_per_axis = {'x': 600,'y': 400,'z': 125,'a': 125,'b': 40,'c': 40}
max_speed_per_axis = {'x': 600,'y': 400,'z': 125,'a': 125,'b': 40,'c': 40}
robot.head_speed(**max_speed_per_axis)
'''
database.delete_container('Falcon_Samples')
database.delete_container('Eppendorf_Samples')
'''
plate_falcon = 'Falcon_Samples'
if plate_falcon not in labware.list():
Falcon = labware.create(
plate_falcon,
grid = (3,2),
spacing = (35,43),
diameter = 10,
depth = 110,
volume = 800)
plate_eppendorf = 'Eppendorf_Samples'
if plate_eppendorf not in labware.list():
}
# [0] Ninja-PCR API
URL_PCR = "http://ninjapcr.local/command"
URL_AUX = "http://thermaux.local/command"
# [1] Labware
# X,Y,Z,A speeds for lateral,front and vertical motion for left and right
# B,C plunger speed for motor
max_speed_per_axis = {'X': 600,'Y': 400,'Z': 125,'A': 125,'B': 40,'C': 40}
robot.head_speed(**max_speed_per_axis)
database.delete_container('Thermic_Module')
thermic_name = 'Thermic_Module' #TODO check
if thermic_name not in labware.list():
thermic = labware.create(
thermic_name, # Labware Name
grid = (4, 4), # Amount of (columns, rows)
spacing = (9, 9), # Distances (mm) between each (column, row)
diameter = 2, # Diameter (mm) of each well on the plate
depth = 10, # Depth (mm) of each well on the plate
volume = 50)
plate_samples = labware.load('96-flat', slot ='11') # Samples TODO Eppendorf 1.5
tiprack = labware.load('tiprack-10ul', slot ='6') # Tipracks
magnetic = modules.load('magdeck', slot ='4') # Magnetic Deck
plate_magnet = labware.load('96-flat', slot ='4', share = True) # Magnetic Deck plate
from opentrons.data_storage import database
metadata = {
'protocolName':
'delete_labware_Starlab',
'author':
'James Kitson <*****@*****.**>',
'description':
'A list of the labware delete commands for standard Starlab consumables'
}
#Deepwell plates
#database.delete_container('starlab-E2896-0220')
#database.delete_container('starlab-E2896-0600')
#RPT filter tips
database.delete_container('tiprack-starlab-S1181-3810')
#database.delete_container('tiprack-starlab-S1180-3810')
#database.delete_container('tiprack-starlab-S1180-1840')
#database.delete_container('tiprack-starlab-S1180-8810')
#database.delete_container('tiprack-starlab-S1180-9810')
#database.delete_container('tiprack-starlab-S1182-1830')
#Regular filter tips
#database.delete_container('tiprack-starlab-S1121-3810')
#database.delete_container('tiprack-starlab-S1120-3810')
#database.delete_container('tiprack-starlab-S1120-2810')
#database.delete_container('tiprack-starlab-S1123-1840')
#database.delete_container('tiprack-starlab-S1120-8810')
#database.delete_container('tiprack-starlab-S1120-9810')
#database.delete_container('tiprack-starlab-S1122-1830')