def test_turn_off_temp_deck(monkeypatch):
import types
from opentrons.drivers.temp_deck import TempDeck
temp_deck = TempDeck()
temp_deck.simulating = False
command_log = []
def _mock_send_command(self, command, timeout=None):
nonlocal command_log
command_log += [command]
return ''
temp_deck._send_command = types.MethodType(_mock_send_command, temp_deck)
temp_deck.disengage()
assert command_log == ['M18']
P300.pick_up_tip()
P300.transfer(200, Culture('A1'), target1(), blow_out=True)
robot.comment(
"Time to centrifuge your cultures"
) # Pause to allow time for bacteria to be pelleted via centrifuge
robot.pause()
# Wash Aliquot
for i in range(1): # Wash step
P300.pick_up_tip(
) # Supernatant is removed from each well to leave the pellet
P300.transfer(200, target1(), Culture('A1').top(-27), blow_out=True)
P300.drop_tip()
P300.pick_up_tip(
) # Wash buffer is added to each individual well and mixing occurs
P300.transfer( # to resuspend the pellet
100,
Buffers_positions,
target1(),
mix_after=(5, 100),
new_tip='always')
robot.comment(
"Time to incubate your cultures"
) # Tempdeck recools and switches off while allowing time for centrifugation
robot.comment("Time to centrifuge your cultures") # of cultures
robot.pause()
# Can be frozen or used immediately depending on your wash buffer
tempdeck.disengage()
robot.comment("Protocol Finished")