def test_multichannel_transfer_old_version(loop):
# for API version below 2.2, multichannel pipette can only
# reach row A of 384-well plates
ctx = papi.ProtocolContext(loop, api_version=APIVersion(2, 1))
lw1 = ctx.load_labware('biorad_96_wellplate_200ul_pcr', 1)
lw2 = ctx.load_labware('corning_384_wellplate_112ul_flat', 2)
tiprack = ctx.load_labware('opentrons_96_tiprack_300ul', 3)
instr_multi = ctx.load_instrument('p300_multi',
Mount.LEFT,
tip_racks=[tiprack])
xfer_plan = tx.TransferPlan(
100,
lw1.rows()[0][0],
[lw2.rows()[0][1], lw2.rows()[1][1]],
instr_multi,
max_volume=instr_multi.hw_pipette['working_volume'],
api_version=ctx.api_version)
xfer_plan_list = []
for step in xfer_plan:
xfer_plan_list.append(step)
exp1 = [{
'method': 'pick_up_tip',
'args': [],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [100, lw1.wells_by_name()['A1'], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [100, lw2.wells_by_index()['A2'], 1.0],
'kwargs': {}
}, {
'method': 'drop_tip',
'args': [],
'kwargs': {}
}]
assert xfer_plan_list == exp1
# target without row limit
with pytest.raises(IndexError):
xfer_plan = tx.TransferPlan(
100,
lw1.rows()[0][1],
lw2.rows()[1][1],
instr_multi,
max_volume=instr_multi.hw_pipette['working_volume'],
api_version=ctx.api_version)
xfer_plan_list = []
for step in xfer_plan:
xfer_plan_list.append(step)
def test_no_new_tip(_instr_labware):
_instr_labware['ctx'].home()
lw1 = _instr_labware['lw1']
lw2 = _instr_labware['lw2']
options = tx.TransferOptions()
options = options._replace(transfer=options.transfer._replace(
new_tip=TransferTipPolicy.NEVER))
# ========== Transfer ==========
xfer_plan = tx.TransferPlan(
100,
lw1.columns()[0],
lw2.columns()[0],
_instr_labware['instr'],
max_volume=_instr_labware['instr'].hw_pipette['working_volume'],
api_version=_instr_labware['ctx'].api_version,
options=options)
for step in xfer_plan:
assert step['method'] != 'pick_up_tip'
assert step['method'] != 'drop_tip'
# ========== Distribute ===========
dist_plan = tx.TransferPlan(
30,
lw1.columns()[0][0],
lw2.columns()[0],
_instr_labware['instr'],
max_volume=_instr_labware['instr'].hw_pipette['working_volume'],
api_version=_instr_labware['ctx'].api_version,
options=options)
for step in dist_plan:
assert step['method'] != 'pick_up_tip'
assert step['method'] != 'drop_tip'
# ========== Consolidate ===========
consd_plan = tx.TransferPlan(
40,
lw1.columns()[0],
lw2.rows()[0][1],
_instr_labware['instr'],
max_volume=_instr_labware['instr'].hw_pipette['working_volume'],
api_version=_instr_labware['ctx'].api_version,
options=options)
for step in consd_plan:
assert step['method'] != 'pick_up_tip'
assert step['method'] != 'drop_tip'
def test_location_wells(_instr_labware):
_instr_labware['ctx'].home()
lw1 = _instr_labware['lw1']
lw2 = _instr_labware['lw2']
aspirate_loc = lw1.wells()[0].top()
# Test single-channel transfer with locations
list_of_locs = [
well.bottom(5) for col in lw2.columns()[0:11] for well in col
]
xfer_plan = tx.TransferPlan(
30,
aspirate_loc,
list_of_locs,
_instr_labware['instr'],
max_volume=_instr_labware['instr'].hw_pipette['working_volume'],
api_version=_instr_labware['ctx'].api_version,
mode='transfer')
idx_dest = 0
for step in xfer_plan:
if step['method'] == 'aspirate':
assert step['args'][1].point == aspirate_loc.point
elif step['method'] == 'dispense':
assert step['args'][1].point\
== list_of_locs[idx_dest].point
idx_dest += 1
multi_locs = [col[0].bottom(5) for col in lw2.columns()[0:11]]
# Test multi-channel transfer with locations
xfer_plan = tx.TransferPlan(
30,
aspirate_loc,
multi_locs,
_instr_labware['instr_multi'],
max_volume=_instr_labware['instr_multi'].hw_pipette['working_volume'],
api_version=_instr_labware['ctx'].api_version,
mode='transfer')
idx_dest = 0
for step in xfer_plan:
if step['method'] == 'aspirate':
assert step['args'][1].point == aspirate_loc.point
elif step['method'] == 'dispense':
assert step['args'][1].point\
== multi_locs[idx_dest].point
idx_dest += 1
def test_all_options(_instr_labware):
_instr_labware['ctx'].home()
lw1 = _instr_labware['lw1']
lw2 = _instr_labware['lw2']
options = tx.TransferOptions()
options = options._replace(
transfer=options.transfer._replace(
new_tip=TransferTipPolicy.ONCE,
drop_tip_strategy=tx.DropTipStrategy.RETURN,
touch_tip_strategy=tx.TouchTipStrategy.ALWAYS,
mix_strategy=tx.MixStrategy.AFTER),
pick_up_tip=options.pick_up_tip._replace(
presses=4,
increment=2),
touch_tip=options.touch_tip._replace(
speed=1.6),
mix=options.mix._replace(
mix_after=options.mix.mix_after._replace(
repetitions=2)),
blow_out=options.blow_out._replace(
location=lw2.columns()[10][0]),
aspirate=options.aspirate._replace(
rate=1.5))
xfer_plan = tx.TransferPlan(
100, lw1.columns()[0][1:4], lw2.rows()[0][1:4],
_instr_labware['instr'],
max_volume=_instr_labware['instr'].hw_pipette['working_volume'],
options=options)
xfer_plan_list = []
for step in xfer_plan:
xfer_plan_list.append(step)
exp1 = [{'method': 'pick_up_tip',
'args': [], 'kwargs': {'presses': 4, 'increment': 2}},
{'method': 'aspirate',
'args': [100, lw1.columns()[0][1], 1.5], 'kwargs': {}},
{'method': 'touch_tip', 'args': [], 'kwargs': {'speed': 1.6}},
{'method': 'dispense',
'args': [100, lw2.rows()[0][1], 1.0], 'kwargs': {}},
{'method': 'mix', 'args': [], 'kwargs': {'repetitions': 2}},
{'method': 'touch_tip', 'args': [], 'kwargs': {'speed': 1.6}},
{'method': 'aspirate',
'args': [100, lw1.columns()[0][2], 1.5], 'kwargs': {}},
{'method': 'touch_tip', 'args': [], 'kwargs': {'speed': 1.6}},
{'method': 'dispense',
'args': [100, lw2.rows()[0][2], 1.0], 'kwargs': {}},
{'method': 'mix', 'args': [], 'kwargs': {'repetitions': 2}},
{'method': 'touch_tip', 'args': [], 'kwargs': {'speed': 1.6}},
{'method': 'aspirate',
'args': [100, lw1.columns()[0][3], 1.5], 'kwargs': {}},
{'method': 'touch_tip', 'args': [], 'kwargs': {'speed': 1.6}},
{'method': 'dispense',
'args': [100, lw2.rows()[0][3], 1.0], 'kwargs': {}},
{'method': 'mix', 'args': [], 'kwargs': {'repetitions': 2}},
{'method': 'touch_tip', 'args': [], 'kwargs': {'speed': 1.6}},
{'method': 'return_tip', 'args': [], 'kwargs': {}}]
assert xfer_plan_list == exp1
def test_new_tip_always(_instr_labware, monkeypatch):
_instr_labware['ctx'].home()
lw1 = _instr_labware['lw1']
lw2 = _instr_labware['lw2']
tiprack = _instr_labware['tiprack']
i_ctx = _instr_labware['instr']
options = tx.TransferOptions()
options = options._replace(
transfer=options.transfer._replace(
new_tip=TransferTipPolicy.ALWAYS,
drop_tip_strategy=tx.DropTipStrategy.TRASH))
xfer_plan = tx.TransferPlan(
100,
lw1.columns()[0][1:5], lw2.columns()[0][1:5],
_instr_labware['instr'],
max_volume=_instr_labware['instr'].hw_pipette['working_volume'],
options=options)
xfer_plan_list = []
for step in xfer_plan:
xfer_plan_list.append(step)
exp1 = [{'method': 'pick_up_tip', 'args': [], 'kwargs': {}},
{'method': 'aspirate', 'args': [100, lw1.columns()[0][1], 1.0],
'kwargs': {}},
{'method': 'dispense', 'args': [100, lw2.columns()[0][1], 1.0],
'kwargs': {}},
{'method': 'drop_tip', 'args': [], 'kwargs': {}},
{'method': 'pick_up_tip', 'args': [], 'kwargs': {}},
{'method': 'aspirate', 'args': [100, lw1.columns()[0][2], 1.0],
'kwargs': {}},
{'method': 'dispense', 'args': [100, lw2.columns()[0][2], 1.0],
'kwargs': {}},
{'method': 'drop_tip', 'args': [], 'kwargs': {}},
{'method': 'pick_up_tip', 'args': [], 'kwargs': {}},
{'method': 'aspirate', 'args': [100, lw1.columns()[0][3], 1.0],
'kwargs': {}},
{'method': 'dispense', 'args': [100, lw2.columns()[0][3], 1.0],
'kwargs': {}},
{'method': 'drop_tip', 'args': [], 'kwargs': {}},
{'method': 'pick_up_tip', 'args': [], 'kwargs': {}},
{'method': 'aspirate', 'args': [100, lw1.columns()[0][4], 1.0],
'kwargs': {}},
{'method': 'dispense', 'args': [100, lw2.columns()[0][4], 1.0],
'kwargs': {}},
{'method': 'drop_tip', 'args': [], 'kwargs': {}}]
assert xfer_plan_list == exp1
for cmd in xfer_plan_list:
getattr(i_ctx, cmd['method'])(*cmd['args'], **cmd['kwargs'])
assert tiprack.next_tip() == tiprack.columns()[0][4]
def test_oversized_consolidate(_instr_labware):
_instr_labware['ctx'].home()
lw1 = _instr_labware['lw1']
lw2 = _instr_labware['lw2']
xfer_plan = tx.TransferPlan(
700, lw2.rows()[0][1:3],
lw1.wells_by_index()['A1'],
_instr_labware['instr'],
max_volume=_instr_labware['instr'].hw_pipette['working_volume'])
xfer_plan_list = []
for step in xfer_plan:
xfer_plan_list.append(step)
exp1 = [{'method': 'pick_up_tip', 'args': [], 'kwargs': {}},
{'method': 'aspirate',
'args': [300, lw2.wells_by_index()['A2'], 1.0], 'kwargs': {}},
{'method': 'dispense',
'args': [300, lw1.wells_by_index()['A1'], 1.0], 'kwargs': {}},
{'method': 'aspirate',
'args': [200, lw2.wells_by_index()['A2'], 1.0], 'kwargs': {}},
{'method': 'dispense',
'args': [200, lw1.wells_by_index()['A1'], 1.0], 'kwargs': {}},
{'method': 'aspirate',
'args': [200, lw2.wells_by_index()['A2'], 1.0], 'kwargs': {}},
{'method': 'dispense',
'args': [200, lw1.wells_by_index()['A1'], 1.0], 'kwargs': {}},
{'method': 'aspirate',
'args': [300, lw2.wells_by_index()['A3'], 1.0], 'kwargs': {}},
{'method': 'dispense',
'args': [300, lw1.wells_by_index()['A1'], 1.0], 'kwargs': {}},
{'method': 'aspirate',
'args': [200, lw2.wells_by_index()['A3'], 1.0], 'kwargs': {}},
{'method': 'dispense',
'args': [200, lw1.wells_by_index()['A1'], 1.0], 'kwargs': {}},
{'method': 'aspirate',
'args': [200, lw2.wells_by_index()['A3'], 1.0], 'kwargs': {}},
{'method': 'dispense',
'args': [200, lw1.wells_by_index()['A1'], 1.0], 'kwargs': {}},
{'method': 'drop_tip', 'args': [], 'kwargs': {}}]
assert xfer_plan_list == exp1
def test_touchtip_mix(_instr_labware):
_instr_labware['ctx'].home()
lw1 = _instr_labware['lw1']
lw2 = _instr_labware['lw2']
options = tx.TransferOptions()
options = options._replace(transfer=options.transfer._replace(
new_tip=TransferTipPolicy.NEVER,
touch_tip_strategy=tx.TouchTipStrategy.ALWAYS,
mix_strategy=tx.MixStrategy.AFTER))
# ========== Transfer ==========
xfer_plan = tx.TransferPlan(
100,
lw1.columns()[0][1:5],
lw2.rows()[0][1:5],
_instr_labware['instr'],
max_volume=_instr_labware['instr'].hw_pipette['working_volume'],
options=options)
xfer_plan_list = []
for step in xfer_plan:
xfer_plan_list.append(step)
exp1 = [{
'method': 'aspirate',
'args': [100, lw1.columns()[0][1], 1.0],
'kwargs': {}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}, {
'method': 'dispense',
'args': [100, lw2.rows()[0][1], 1.0],
'kwargs': {}
}, {
'method': 'mix',
'args': [],
'kwargs': {
'location': lw2.rows()[0][1]
}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [100, lw1.columns()[0][2], 1.0],
'kwargs': {}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}, {
'method': 'dispense',
'args': [100, lw2.rows()[0][2], 1.0],
'kwargs': {}
}, {
'method': 'mix',
'args': [],
'kwargs': {
'location': lw2.rows()[0][2]
}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [100, lw1.columns()[0][3], 1.0],
'kwargs': {}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}, {
'method': 'dispense',
'args': [100, lw2.rows()[0][3], 1.0],
'kwargs': {}
}, {
'method': 'mix',
'args': [],
'kwargs': {
'location': lw2.rows()[0][3]
}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [100, lw1.columns()[0][4], 1.0],
'kwargs': {}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}, {
'method': 'dispense',
'args': [100, lw2.rows()[0][4], 1.0],
'kwargs': {}
}, {
'method': 'mix',
'args': [],
'kwargs': {
'location': lw2.rows()[0][4]
}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}]
assert xfer_plan_list == exp1
# ========== Distribute ==========
dist_plan = tx.TransferPlan(
60,
lw1.columns()[1][0],
lw2.rows()[1][1:6],
_instr_labware['instr'],
max_volume=_instr_labware['instr'].hw_pipette['working_volume'],
options=options)
dist_plan_list = []
for step in dist_plan:
dist_plan_list.append(step)
exp2 = [{
'method': 'aspirate',
'args': [300, lw1.columns()[1][0], 1.0],
'kwargs': {}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}, {
'method': 'dispense',
'args': [60, lw2.rows()[1][1], 1.0],
'kwargs': {}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}, {
'method': 'dispense',
'args': [60, lw2.rows()[1][2], 1.0],
'kwargs': {}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}, {
'method': 'dispense',
'args': [60, lw2.rows()[1][3], 1.0],
'kwargs': {}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}, {
'method': 'dispense',
'args': [60, lw2.rows()[1][4], 1.0],
'kwargs': {}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}, {
'method': 'dispense',
'args': [60, lw2.rows()[1][5], 1.0],
'kwargs': {}
}, {
'method': 'mix',
'args': [],
'kwargs': {
'location': lw2.rows()[1][5]
}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}]
assert dist_plan_list == exp2
# ========== Consolidate ==========
consd_plan = tx.TransferPlan(
60,
lw1.columns()[1],
lw2.rows()[1][1],
_instr_labware['instr'],
max_volume=_instr_labware['instr'].hw_pipette['working_volume'],
options=options)
consd_plan_list = []
for step in consd_plan:
consd_plan_list.append(step)
exp3 = [{
'method': 'aspirate',
'args': [60, lw1.columns()[1][0], 1.0],
'kwargs': {}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [60, lw1.columns()[1][1], 1.0],
'kwargs': {}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [60, lw1.columns()[1][2], 1.0],
'kwargs': {}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [60, lw1.columns()[1][3], 1.0],
'kwargs': {}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [60, lw1.columns()[1][4], 1.0],
'kwargs': {}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}, {
'method': 'dispense',
'args': [300, lw2.rows()[1][1], 1.0],
'kwargs': {}
}, {
'method': 'mix',
'args': [],
'kwargs': {
'location': lw2.rows()[1][1]
}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [60, lw1.columns()[1][5], 1.0],
'kwargs': {}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [60, lw1.columns()[1][6], 1.0],
'kwargs': {}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [60, lw1.columns()[1][7], 1.0],
'kwargs': {}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}, {
'method': 'dispense',
'args': [180, lw2.rows()[1][1], 1.0],
'kwargs': {}
}, {
'method': 'mix',
'args': [],
'kwargs': {
'location': lw2.rows()[1][1]
}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}]
assert consd_plan_list == exp3
def test_transfer_w_airgap_blowout(_instr_labware):
_instr_labware['ctx'].home()
lw1 = _instr_labware['lw1']
lw2 = _instr_labware['lw2']
options = tx.TransferOptions()
options = options._replace(transfer=options.transfer._replace(
air_gap=10,
blow_out_strategy=tx.BlowOutStrategy.DEST_IF_EMPTY,
new_tip=TransferTipPolicy.NEVER))
# ========== Transfer ==========
xfer_plan = tx.TransferPlan(
100,
lw1.columns()[0][1:5],
lw2.rows()[0][1:5],
_instr_labware['instr'],
max_volume=_instr_labware['instr'].hw_pipette['working_volume'],
options=options)
xfer_plan_list = []
for step in xfer_plan:
xfer_plan_list.append(step)
exp1 = [{
'method': 'aspirate',
'args': [100, lw1.columns()[0][1], 1.0],
'kwargs': {}
}, {
'method': 'air_gap',
'args': [10],
'kwargs': {}
}, {
'method': 'dispense',
'args': [10],
'kwargs': {}
}, {
'method': 'dispense',
'args': [100, lw2.rows()[0][1], 1.0],
'kwargs': {}
}, {
'method': 'blow_out',
'args': [lw2.rows()[0][1]],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [100, lw1.columns()[0][2], 1.0],
'kwargs': {}
}, {
'method': 'air_gap',
'args': [10],
'kwargs': {}
}, {
'method': 'dispense',
'args': [10],
'kwargs': {}
}, {
'method': 'dispense',
'args': [100, lw2.rows()[0][2], 1.0],
'kwargs': {}
}, {
'method': 'blow_out',
'args': [lw2.rows()[0][2]],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [100, lw1.columns()[0][3], 1.0],
'kwargs': {}
}, {
'method': 'air_gap',
'args': [10],
'kwargs': {}
}, {
'method': 'dispense',
'args': [10],
'kwargs': {}
}, {
'method': 'dispense',
'args': [100, lw2.rows()[0][3], 1.0],
'kwargs': {}
}, {
'method': 'blow_out',
'args': [lw2.rows()[0][3]],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [100, lw1.columns()[0][4], 1.0],
'kwargs': {}
}, {
'method': 'air_gap',
'args': [10],
'kwargs': {}
}, {
'method': 'dispense',
'args': [10],
'kwargs': {}
}, {
'method': 'dispense',
'args': [100, lw2.rows()[0][4], 1.0],
'kwargs': {}
}, {
'method': 'blow_out',
'args': [lw2.rows()[0][4]],
'kwargs': {}
}]
assert xfer_plan_list == exp1
# ========== Distribute ==========
dist_plan = tx.TransferPlan(
60,
lw1.columns()[1][0],
lw2.rows()[1][1:6],
_instr_labware['instr'],
max_volume=_instr_labware['instr'].hw_pipette['working_volume'],
options=options)
dist_plan_list = []
for step in dist_plan:
dist_plan_list.append(step)
exp2 = [{
'method': 'aspirate',
'args': [240, lw1.columns()[1][0], 1.0],
'kwargs': {}
}, {
'method': 'air_gap',
'args': [10],
'kwargs': {}
}, {
'method': 'dispense',
'args': [10],
'kwargs': {}
}, {
'method': 'dispense',
'args': [60, lw2.rows()[1][1], 1.0],
'kwargs': {}
}, {
'method': 'air_gap',
'args': [10],
'kwargs': {}
}, {
'method': 'dispense',
'args': [10],
'kwargs': {}
}, {
'method': 'dispense',
'args': [60, lw2.rows()[1][2], 1.0],
'kwargs': {}
}, {
'method': 'air_gap',
'args': [10],
'kwargs': {}
}, {
'method': 'dispense',
'args': [10],
'kwargs': {}
}, {
'method': 'dispense',
'args': [60, lw2.rows()[1][3], 1.0],
'kwargs': {}
}, {
'method': 'air_gap',
'args': [10],
'kwargs': {}
}, {
'method': 'dispense',
'args': [10],
'kwargs': {}
}, {
'method': 'dispense',
'args': [60, lw2.rows()[1][4], 1.0],
'kwargs': {}
}, {
'method': 'blow_out',
'args': [lw2.rows()[1][4]],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [60, lw1.columns()[1][0], 1.0],
'kwargs': {}
}, {
'method': 'air_gap',
'args': [10],
'kwargs': {}
}, {
'method': 'dispense',
'args': [10],
'kwargs': {}
}, {
'method': 'dispense',
'args': [60, lw2.rows()[1][5], 1.0],
'kwargs': {}
}, {
'method': 'blow_out',
'args': [lw2.rows()[1][5]],
'kwargs': {}
}]
assert dist_plan_list == exp2
# ========== Consolidate ==========
consd_plan = tx.TransferPlan(
60,
lw1.columns()[1],
lw2.rows()[1][1],
_instr_labware['instr'],
max_volume=_instr_labware['instr'].hw_pipette['working_volume'],
options=options)
consd_plan_list = []
for step in consd_plan:
consd_plan_list.append(step)
exp3 = [{
'method': 'aspirate',
'args': [60, lw1.columns()[1][0], 1.0],
'kwargs': {}
}, {
'method': 'air_gap',
'args': [10],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [60, lw1.columns()[1][1], 1.0],
'kwargs': {}
}, {
'method': 'air_gap',
'args': [10],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [60, lw1.columns()[1][2], 1.0],
'kwargs': {}
}, {
'method': 'air_gap',
'args': [10],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [60, lw1.columns()[1][3], 1.0],
'kwargs': {}
}, {
'method': 'air_gap',
'args': [10],
'kwargs': {}
}, {
'method': 'dispense',
'args': [10],
'kwargs': {}
}, {
'method': 'dispense',
'args': [270, lw2.rows()[1][1], 1.0],
'kwargs': {}
}, {
'method': 'blow_out',
'args': [lw2.rows()[1][1]],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [60, lw1.columns()[1][4], 1.0],
'kwargs': {}
}, {
'method': 'air_gap',
'args': [10],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [60, lw1.columns()[1][5], 1.0],
'kwargs': {}
}, {
'method': 'air_gap',
'args': [10],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [60, lw1.columns()[1][6], 1.0],
'kwargs': {}
}, {
'method': 'air_gap',
'args': [10],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [60, lw1.columns()[1][7], 1.0],
'kwargs': {}
}, {
'method': 'air_gap',
'args': [10],
'kwargs': {}
}, {
'method': 'dispense',
'args': [10],
'kwargs': {}
}, {
'method': 'dispense',
'args': [270, lw2.rows()[1][1], 1.0],
'kwargs': {}
}, {
'method': 'blow_out',
'args': [lw2.rows()[1][1]],
'kwargs': {}
}]
assert consd_plan_list == exp3
def test_default_transfers(_instr_labware):
# Transfer 100ml from row1 of labware1 to row1 of labware2: first with
# new_tip = ONCE, then with new_tip = NEVER
_instr_labware['ctx'].home()
lw1 = _instr_labware['lw1']
lw2 = _instr_labware['lw2']
# ========== Transfer ===========
xfer_plan = tx.TransferPlan(
100,
lw1.columns()[0],
lw2.columns()[0],
_instr_labware['instr'],
max_volume=_instr_labware['instr'].hw_pipette['working_volume'])
xfer_plan_list = []
for step in xfer_plan:
xfer_plan_list.append(step)
exp1 = [{
'method': 'pick_up_tip',
'args': [],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [100, lw1.columns()[0][0], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [100, lw2.columns()[0][0], 1.0],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [100, lw1.columns()[0][1], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [100, lw2.columns()[0][1], 1.0],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [100, lw1.columns()[0][2], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [100, lw2.columns()[0][2], 1.0],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [100, lw1.columns()[0][3], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [100, lw2.columns()[0][3], 1.0],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [100, lw1.columns()[0][4], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [100, lw2.columns()[0][4], 1.0],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [100, lw1.columns()[0][5], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [100, lw2.columns()[0][5], 1.0],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [100, lw1.columns()[0][6], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [100, lw2.columns()[0][6], 1.0],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [100, lw1.columns()[0][7], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [100, lw2.columns()[0][7], 1.0],
'kwargs': {}
}, {
'method': 'drop_tip',
'args': [],
'kwargs': {}
}]
assert xfer_plan_list == exp1
# ========== Distribute ===========
dist_plan = tx.TransferPlan(
50,
lw1.columns()[0][0],
lw2.columns()[0],
_instr_labware['instr'],
max_volume=_instr_labware['instr'].hw_pipette['working_volume'])
dist_plan_list = []
for step in dist_plan:
dist_plan_list.append(step)
exp2 = [{
'method': 'pick_up_tip',
'args': [],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [300, lw1.columns()[0][0], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [50, lw2.columns()[0][0], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [50, lw2.columns()[0][1], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [50, lw2.columns()[0][2], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [50, lw2.columns()[0][3], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [50, lw2.columns()[0][4], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [50, lw2.columns()[0][5], 1.0],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [100, lw1.columns()[0][0], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [50, lw2.columns()[0][6], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [50, lw2.columns()[0][7], 1.0],
'kwargs': {}
}, {
'method': 'drop_tip',
'args': [],
'kwargs': {}
}]
assert dist_plan_list == exp2
# ========== Consolidate ===========
consd_plan = tx.TransferPlan(
50,
lw1.columns()[0],
lw2.columns()[0][0],
_instr_labware['instr'],
max_volume=_instr_labware['instr'].hw_pipette['working_volume'])
consd_plan_list = []
for step in consd_plan:
consd_plan_list.append(step)
exp3 = [{
'method': 'pick_up_tip',
'args': [],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [50, lw1.columns()[0][0], 1.0],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [50, lw1.columns()[0][1], 1.0],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [50, lw1.columns()[0][2], 1.0],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [50, lw1.columns()[0][3], 1.0],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [50, lw1.columns()[0][4], 1.0],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [50, lw1.columns()[0][5], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [300, lw2.columns()[0][0], 1.0],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [50, lw1.columns()[0][6], 1.0],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [50, lw1.columns()[0][7], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [100, lw2.columns()[0][0], 1.0],
'kwargs': {}
}, {
'method': 'drop_tip',
'args': [],
'kwargs': {}
}]
assert consd_plan_list == exp3
def test_uneven_transfers(_instr_labware):
_instr_labware['ctx'].home()
lw1 = _instr_labware['lw1']
lw2 = _instr_labware['lw2']
options = tx.TransferOptions()
options = options._replace(transfer=options.transfer._replace(
new_tip=TransferTipPolicy.NEVER))
# ========== One-to-Many ==========
xfer_plan = tx.TransferPlan(
100,
lw1.columns()[0][0],
lw2.columns()[1][:4],
_instr_labware['instr'],
max_volume=_instr_labware['instr'].hw_pipette['working_volume'],
mode='transfer',
options=options)
one_to_many_plan_list = []
for step in xfer_plan:
one_to_many_plan_list.append(step)
exp1 = [{
'method': 'aspirate',
'args': [100, lw1.columns()[0][0], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [100, lw2.columns()[1][0], 1.0],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [100, lw1.columns()[0][0], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [100, lw2.columns()[1][1], 1.0],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [100, lw1.columns()[0][0], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [100, lw2.columns()[1][2], 1.0],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [100, lw1.columns()[0][0], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [100, lw2.columns()[1][3], 1.0],
'kwargs': {}
}]
assert one_to_many_plan_list == exp1
# ========== Few-to-Many ==========
xfer_plan = tx.TransferPlan(
[100, 90, 80, 70],
lw1.columns()[0][:2],
lw2.columns()[1][:4],
_instr_labware['instr'],
max_volume=_instr_labware['instr'].hw_pipette['working_volume'],
mode='transfer',
options=options)
few_to_many_plan_list = []
for step in xfer_plan:
few_to_many_plan_list.append(step)
exp2 = [{
'method': 'aspirate',
'args': [100, lw1.columns()[0][0], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [100, lw2.columns()[1][0], 1.0],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [90, lw1.columns()[0][0], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [90, lw2.columns()[1][1], 1.0],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [80, lw1.columns()[0][1], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [80, lw2.columns()[1][2], 1.0],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [70, lw1.columns()[0][1], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [70, lw2.columns()[1][3], 1.0],
'kwargs': {}
}]
assert few_to_many_plan_list == exp2
# ========== Many-to-One ==========
xfer_plan = tx.TransferPlan(
[100, 90, 80, 70],
lw1.columns()[0][:4],
lw2.columns()[1][0],
_instr_labware['instr'],
max_volume=_instr_labware['instr'].hw_pipette['working_volume'],
mode='transfer',
options=options)
many_to_one_plan_list = []
for step in xfer_plan:
many_to_one_plan_list.append(step)
exp3 = [{
'method': 'aspirate',
'args': [100, lw1.columns()[0][0], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [100, lw2.columns()[1][0], 1.0],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [90, lw1.columns()[0][1], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [90, lw2.columns()[1][0], 1.0],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [80, lw1.columns()[0][2], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [80, lw2.columns()[1][0], 1.0],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [70, lw1.columns()[0][3], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [70, lw2.columns()[1][0], 1.0],
'kwargs': {}
}]
assert many_to_one_plan_list == exp3
def test_transfer_w_touchtip_blowout(_instr_labware):
_instr_labware['ctx'].home()
lw1 = _instr_labware['lw1']
lw2 = _instr_labware['lw2']
# ========== Transfer ==========
options = tx.TransferOptions()
options = options._replace(transfer=options.transfer._replace(
touch_tip_strategy=tx.TouchTipStrategy.ALWAYS,
blow_out_strategy=tx.BlowOutStrategy.TRASH,
new_tip=TransferTipPolicy.NEVER))
xfer_plan = tx.TransferPlan(
100,
lw1.columns()[0][:3],
lw2.rows()[0][:3],
_instr_labware['instr'],
max_volume=_instr_labware['instr'].hw_pipette['working_volume'],
options=options)
xfer_plan_list = []
for step in xfer_plan:
xfer_plan_list.append(step)
exp1 = [{
'method': 'aspirate',
'args': [100, lw1.columns()[0][0], 1.0],
'kwargs': {}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}, {
'method': 'dispense',
'args': [100, lw2.rows()[0][0], 1.0],
'kwargs': {}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}, {
'method': 'blow_out',
'args': [_instr_labware['instr'].trash_container.wells()[0]],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [100, lw1.columns()[0][1], 1.0],
'kwargs': {}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}, {
'method': 'dispense',
'args': [100, lw2.rows()[0][1], 1.0],
'kwargs': {}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}, {
'method': 'blow_out',
'args': [_instr_labware['instr'].trash_container.wells()[0]],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [100, lw1.columns()[0][2], 1.0],
'kwargs': {}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}, {
'method': 'dispense',
'args': [100, lw2.rows()[0][2], 1.0],
'kwargs': {}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}, {
'method': 'blow_out',
'args': [_instr_labware['instr'].trash_container.wells()[0]],
'kwargs': {}
}]
assert xfer_plan_list == exp1
# ========== Distribute ==========
options = tx.TransferOptions()
options = options._replace(transfer=options.transfer._replace(
disposal_volume=_instr_labware['instr'].min_volume,
touch_tip_strategy=tx.TouchTipStrategy.ALWAYS,
new_tip=TransferTipPolicy.NEVER))
dist_plan = tx.TransferPlan(
30,
lw1.columns()[0][0],
lw2.rows()[0][:3],
_instr_labware['instr'],
max_volume=_instr_labware['instr'].hw_pipette['working_volume'],
options=options)
dist_plan_list = []
for step in dist_plan:
dist_plan_list.append(step)
exp2 = [{
'method': 'aspirate',
'args': [120, lw1.columns()[0][0], 1.0],
'kwargs': {}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}, {
'method': 'dispense',
'args': [30, lw2.rows()[0][0], 1.0],
'kwargs': {}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}, {
'method': 'dispense',
'args': [30, lw2.rows()[0][1], 1.0],
'kwargs': {}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}, {
'method': 'dispense',
'args': [30, lw2.rows()[0][2], 1.0],
'kwargs': {}
}, {
'method': 'touch_tip',
'args': [],
'kwargs': {}
}, {
'method': 'blow_out',
'args': [_instr_labware['instr'].trash_container.wells()[0]],
'kwargs': {}
}]
assert dist_plan_list == exp2
def test_multichannel_transfer_locs(loop):
ctx = papi.ProtocolContext(loop, api_version=APIVersion(2, 2))
lw1 = ctx.load_labware('biorad_96_wellplate_200ul_pcr', 1)
lw2 = ctx.load_labware('corning_384_wellplate_112ul_flat', 2)
tiprack = ctx.load_labware('opentrons_96_tiprack_300ul', 3)
instr_multi = ctx.load_instrument('p300_multi',
Mount.LEFT,
tip_racks=[tiprack])
# targets within row limit
xfer_plan = tx.TransferPlan(
100,
lw1.rows()[0][1],
lw2.rows()[1][1],
instr_multi,
max_volume=instr_multi.hw_pipette['working_volume'],
api_version=ctx.api_version)
xfer_plan_list = []
for step in xfer_plan:
xfer_plan_list.append(step)
exp1 = [{
'method': 'pick_up_tip',
'args': [],
'kwargs': {}
}, {
'method': 'aspirate',
'args': [100, lw1.wells_by_name()['A2'], 1.0],
'kwargs': {}
}, {
'method': 'dispense',
'args': [100, lw2.wells_by_index()['B2'], 1.0],
'kwargs': {}
}, {
'method': 'drop_tip',
'args': [],
'kwargs': {}
}]
assert xfer_plan_list == exp1
# targets outside of row limit will be skipped
xfer_plan = tx.TransferPlan(
100,
lw1.rows()[0][1],
[lw2.rows()[1][1], lw2.rows()[2][1]],
instr_multi,
max_volume=instr_multi.hw_pipette['working_volume'],
api_version=ctx.api_version)
xfer_plan_list = []
for step in xfer_plan:
xfer_plan_list.append(step)
assert xfer_plan_list == exp1
# no valid source or targets, raise error
with pytest.raises(RuntimeError):
assert tx.TransferPlan(
100,
lw1.rows()[0][1],
lw2.rows()[2][1],
instr_multi,
max_volume=instr_multi.hw_pipette['working_volume'],
api_version=ctx.api_version)
