def __write_general_section(self):
# The general section contains library name, layout number, sector
# index, number of tubes, and transfer volume.
if self.has_pool_stock_racks:
vol = get_pool_transfer_volume(
number_designs=self.iso.iso_request.number_designs)
else:
pp_vol = self.iso.iso_request.preparation_plate_volume \
* VOLUME_CONVERSION_FACTOR
src_conc = self.iso.iso_request.stock_concentration \
* CONCENTRATION_CONVERSION_FACTOR
vol = get_preparation_plate_transfer_volume(
source_concentration=src_conc, preparation_plate_volume=pp_vol)
self._write_headline(self.GENERAL_HEADER, preceding_blank_lines=1)
general_lines = [
self.LIBRARY_LINE % self.iso.iso_request.label,
self.LAYOUT_NUMBER_LINE % self.iso.layout_number
]
if not self.sector_index is None:
general_lines.append(self.SECTOR_NUMBER_LINE %
(self.sector_index + 1))
general_lines.extend([
self.TUBE_NO_LINE % len(self.tube_transfers),
self.VOLUME_LINE % vol
])
self._write_body_lines(general_lines)
def __write_pool_creation_section(self):
# This is the stock transfer part (creating pools from single molecule
# designs).
self.add_debug('Writing pool section.')
self._write_headline(header_text=self.HEADER_POOL_CREATION,
preceding_blank_lines=0)
lines = []
volume_line = self.VOLUME_LINE \
% get_pool_transfer_volume(number_designs=
self.iso.iso_request.number_designs)
volume_line += ' each'
lines.append(volume_line)
buffer_line = self.BUFFER_LINE % get_pool_buffer_volume()
lines.append(buffer_line)
for sector_index in sorted(self.single_stock_rack_map.keys()):
lines.append('')
lines.append(self.QUADRANT_LINE % (sector_index + 1))
barcodes = self.single_stock_rack_map[sector_index]
target_rack = self.pool_stock_rack_map[sector_index]
src_line = self.SOURCE_LINE_PLURAL % (', '.join(sorted(barcodes)))
lines.append(src_line)
lines.append(self.TARGET_LINE % (target_rack))
self._write_body_lines(lines)
def __write_general_section(self):
# The general section contains library name, layout number, sector
# index, number of tubes, and transfer volume.
if self.has_pool_stock_racks:
vol = get_pool_transfer_volume(
number_designs=self.iso.iso_request.number_designs)
else:
pp_vol = self.iso.iso_request.preparation_plate_volume \
* VOLUME_CONVERSION_FACTOR
src_conc = self.iso.iso_request.stock_concentration \
* CONCENTRATION_CONVERSION_FACTOR
vol = get_preparation_plate_transfer_volume(
source_concentration=src_conc,
preparation_plate_volume=pp_vol)
self._write_headline(self.GENERAL_HEADER, preceding_blank_lines=1)
general_lines = [self.LIBRARY_LINE % self.iso.iso_request.label,
self.LAYOUT_NUMBER_LINE % self.iso.layout_number]
if not self.sector_index is None:
general_lines.append(
self.SECTOR_NUMBER_LINE % (self.sector_index + 1))
general_lines.extend([self.TUBE_NO_LINE % len(self.tube_transfers),
self.VOLUME_LINE % vol])
self._write_body_lines(general_lines)
def __write_pool_creation_section(self):
# This is the stock transfer part (creating pools from single molecule
# designs).
self.add_debug('Writing pool section.')
self._write_headline(header_text=self.HEADER_POOL_CREATION,
preceding_blank_lines=0)
lines = []
volume_line = self.VOLUME_LINE \
% get_pool_transfer_volume(number_designs=
self.iso.iso_request.number_designs)
volume_line += ' each'
lines.append(volume_line)
buffer_line = self.BUFFER_LINE % get_pool_buffer_volume()
lines.append(buffer_line)
for sector_index in sorted(self.single_stock_rack_map.keys()):
lines.append('')
lines.append(self.QUADRANT_LINE % (sector_index + 1))
barcodes = self.single_stock_rack_map[sector_index]
target_rack = self.pool_stock_rack_map[sector_index]
src_line = self.SOURCE_LINE_PLURAL % (', '.join(sorted(barcodes)))
lines.append(src_line)
lines.append(self.TARGET_LINE % (target_rack))
self._write_body_lines(lines)