def pat2beta(pat_path, out_dir, args, force=True):
validate_single_file(pat_path)
if pat_path.endswith('.pat.gz'):
cmd = 'gunzip -cd'
elif pat_path.endswith('.pat'):
cmd = 'cat'
else:
raise IllegalArgumentError(f'Invalid pat suffix: {pat_path}')
suff = '.lbeta' if args.lbeta else '.beta'
out_beta = op.join(out_dir, splitextgz(op.basename(pat_path))[0] + suff)
if not delete_or_skip(out_beta, force):
return
if args.threads > 1 and pat_path.endswith('.pat.gz') and op.isfile(
pat_path + '.csi'):
arr = mult_pat2beta(pat_path, args)
else:
nr_sites = GenomeRefPaths(args.genome).get_nr_sites()
cmd += f' {pat_path} | {pat2beta_tool} {1} {nr_sites + 1}'
x = subprocess.check_output(cmd, shell=True).decode()
arr = np.fromstring(x, dtype=int, sep=' ').reshape((-1, 2))
trim_to_uint8(arr, args.lbeta).tofile(out_beta)
return out_beta
def convert_bed_file(args):
"""
bed file should be of the format (tab-separated):
Input:
chr start end [...]
Output:
chr start end startCpG endCpG [...]
"""
out_path = sys.stdout if args.out_path is None else args.out_path
if not delete_or_skip(out_path, args.force):
return
# add CpG columns
bed_file = args.bed_file
# TODO: support stdin for -L in all wgbstools features, and add it to the help message
if bed_file == '-':
bed_file = sys.stdin
add_anno = (not args.parsable) and (not args.no_anno)
if not check_executable('bedtools', verbose=False):
# eprint('continue with a slower implementation')
r = add_cpgs_to_bed(bed_file=bed_file,
genome=args.genome,
drop_empty=args.drop_empty,
threads=args.threads,
add_anno=add_anno)
else:
r = bedtools_conversion(bed_file, args.genome, args.drop_empty,
add_anno, args.debug)
r.to_csv(out_path, sep='\t', header=None, index=None, na_rep='NA')
def main():
"""
Merge files.
Accumulate all reads / observations from multiple (>=2) input files,
and output a single file of the same format.
Supported formats: pat.gz, beta
"""
args = parse_args()
# validate input files
input_files = args.input_files
# construct output path
out_path = args.prefix + splitextgz(args.input_files[0])[1]
if op.realpath(out_path) in [op.realpath(p) for p in args.input_files]:
eprint('[wt merge] Error output path is identical ' \
'to one of the input files {out_path}')
return
if not delete_or_skip(out_path, args.force):
return
files_type = splitextgz(input_files[0])[1][1:]
if files_type in ('beta', 'bin'):
merge_betas(input_files, out_path)
elif files_type == 'pat.gz':
MergePats(input_files, args.prefix + '.pat.gz', args.labels,
args).merge_pats()
else:
print('Unknown input format:', input_files[0])
return
def bed2betas(args):
# merge with the reference CpG bed file,
# so the #lines in file will include all 28217448 sites (with NaN as 0)
region = 'chr1:10469-876225' if args.debug else None
nrows = 10000 if args.debug else None
try:
rf = None # Reference dictionary
for bed in args.bed_paths:
eprint(f'[wt bed] Converting {op.basename(bed)}...')
# Check if bed should be skipped
outpath = op.join(args.outdir, splitextgz(op.basename(bed))[0] + '.beta')
if not delete_or_skip(outpath, args.force):
continue
# Load dict (at most once) and bed
if rf is None:
rf = load_dict_section(region, args.genome)
df = load_bed(bed, nrows, args.add_one)
# todo: implement in C++.
# merge dict with bed, then dump
res = rf.merge(df, how='left', on=['chr', 'start']).fillna(0)
trim_to_uint8(np.array(res[['meth', 'total']])).tofile(outpath)
except pd.errors.ParserError as e:
eprint(f'[wt bed] Invalid input file.\n{e}')
return
def main():
"""
Merge files.
Accumulate all reads / observations from multiple (>=2) input files,
and output a single file of the same format.
Supported formats: pat.gz, beta
"""
args = parse_args()
# validate input files
input_files = args.input_files
validate_files_list(input_files, min_len=2)
# construct output path
out_path = args.prefix + splitextgz(args.input_files[0])[1]
if not delete_or_skip(out_path, args.force):
return
files_type = splitextgz(input_files[0])[1][1:]
if files_type in ('beta', 'bin'):
merge_betas(input_files, out_path)
elif files_type == 'pat.gz':
MergePats(input_files, args.prefix + '.pat', args.labels, args).merge_pats()
elif files_type == 'unq.gz':
merge_unqs()
else:
print('Unknown input format:', input_files[0])
return
def bed2betas(args):
# merge with the reference CpG bed file,
# so the #lines in file will include all 28217448 sites (with NaN as 0)
nrows = 100000 if args.debug else None
try:
rf = None # Reference dictionary
for bed in args.bed_paths:
eprint('Converting {}...'.format(op.basename(bed)))
# Check if bed should be skipped:
outpath = op.join(args.outdir,
splitextgz(op.basename(bed))[0]) + '.beta'
if not delete_or_skip(outpath, args.force):
continue
# Load dict (at most once) and bed
if rf is None:
rf = load_dict(nrows=nrows, genome_name=args.genome)
df = load_bed(bed, nrows, args.genome == 'mm9')
# merge dict with bed, then dump
res = rf.merge(df, how='left', on=['chr', 'start']).fillna(0)
trim_to_uint8(np.array(res[['meth', 'total']])).tofile(outpath)
except pd.errors.ParserError as e:
eprint('Invalid input file.\n{}'.format(e))
return
def beta_to_bed(beta_path, gr, bed_file, min_cov, mean, keep_na, force, opath):
if not delete_or_skip(opath, force):
return
cmd = beta2bed_build_cmd(beta_path, gr, bed_file, min_cov, mean, keep_na)
if opath is not None:
if opath.endswith('.gz'):
cmd += ' | gzip -c '
cmd += f' > {opath}'
subprocess_wrap_sigpipe(cmd)
def convert_site_file(args):
"""
site file should be of the format (tab-separated):
Input:
startCpG [endCpG]
Output:
chr start end startCpG [endCpG]
"""
out_path = sys.stdout if args.out_path is None else args.out_path
if not delete_or_skip(out_path, args.force):
return
# add loci columns
add_bed_to_cpgs(args.site_file, args.genome, args.out_path)
def run(self):
# if index already exists delete it or skip it
if not delete_or_skip(self.in_file + self.ftype.ind_suff, self.force):
return
# if file is gzipped instead of bgzipped, uncompress it
if self.is_file_gzipped():
sp.check_call(['gunzip', self.in_file])
self.in_file = self.in_file[:-3]
if not self.in_file.endswith('.gz'):
self.bgzip()
self.index_bgzipped_file()
def __init__(self, args):
self.args = args
self.out_path = args.out_path
self.debug = args.debug
if not delete_or_skip(self.out_path, self.args.force):
return
# load bed file:
self.df = load_bed(args.bed_path, 100000 if self.debug else None)
self.genome = GenomeRefPaths(args.genome)
# load chromosomes sizes (in GpGs):
self.cf = self.genome.get_chrom_cpg_size_table()
self.cf['size'] = np.cumsum(self.cf['size'])
self.proc_bed()
def __init__(self, args):
self.args = args
self.ref_path = args.genome_ref
self.force = args.force
self.name = args.name
self.out_dir = self.make_output_dir()
# validate input files
validate_single_file(self.ref_path, '.fa')
# abort if files exists and --force was not specified
eprint('Setting up genome reference files in {}'.format(self.out_dir))
if not delete_or_skip(op.join(self.out_dir, 'CpG.bed.gz'), self.force):
return
self.fai_df = self.load_fai()
def run_beta_to_bed(self, beta_path):
eprint('{}'.format(op.basename(beta_path)))
prefix = self.set_prefix(beta_path)
out_bed = prefix + '.bed'
if not delete_or_skip(out_bed, self.args.force):
return
barr = self.load_beta(beta_path)
# paste dict with beta, then dump
self.ref_dict['meth'] = barr[:, 0]
self.ref_dict['total'] = barr[:, 1]
self.ref_dict[self.ref_dict['total'] > 0].to_csv(out_bed,
sep='\t',
header=None,
index=None)
del self.ref_dict['meth'], self.ref_dict['total']
def run(self):
# if index already exists delete it or skip it
if not delete_or_skip(self.in_file + self.ftype.ind_suff,
self.args.force):
return
if self.in_file.endswith('.gz'):
self.in_file = op.splitext(self.in_file)[0]
# try indexing it:
if not self.index_bgzipped_file():
return # success
# couldn't index because the file is gzipped instead of bgzipped
subprocess.check_call(['gunzip', self.in_file + '.gz'])
self.bgzip()
self.index_bgzipped_file()
def single_mix(self, rep):
prefix_i = self.prefix + '_{}.pat'.format(rep + 1)
if not delete_or_skip(prefix_i + '.gz', self.args.force):
return
view_flags = []
for i in range(self.nr_pats):
v = ' --awk '
if self.args.strict:
v += ' --strict'
if self.args.bed_file is not None:
v += ' -L {}'.format(self.args.bed_file)
elif self.gr.sites is not None:
v += ' -s {}-{}'.format(*self.gr.sites)
v += ' --sub_sample {}'.format(self.adj_rates[i])
view_flags.append(v)
print('prefix:', prefix_i)
m = MergePats(self.pats, prefix_i, self.labels, args=self.args)
m.fast_merge_pats(view_flags=view_flags)
def main():
"""
Run the WGBS pipeline to generate pat & beta files out of an input bam file
"""
parser = add_args_snp_splitt()
args = parse_args_snp_split(parser)
# validate output dir:
if not op.isdir(args.out_dir):
raise IllegalArgumentError(f'Invalid output dir: {args.out_dir}')
validate_local_exe(allele_split_tool)
for bam in [args.bam]:
if not validate_bam(bam):
eprint(f'[wt bam2pat] Skipping {bam}')
continue
pat = op.join(args.out_dir, op.basename(bam)[:-4] + PAT_SUFF)
if not delete_or_skip(pat, args.force):
continue
SNPSplit(args, bam)
def pat2beta(pat_path, out_dir, args, force=True):
validate_single_file(pat_path)
if pat_path.endswith('.pat.gz'):
cmd = 'gunzip -cd'
elif pat_path.endswith('.pat'):
cmd = 'cat'
else:
raise IllegalArgumentError('Invalid pat suffix: {}'.format(pat_path))
out_beta = op.join(out_dir, splitextgz(op.basename(pat_path))[0] + '.beta')
if not delete_or_skip(out_beta, force):
return
nr_sites = GenomeRefPaths(args.genome).nr_sites
if args.threads > 1 and pat_path.endswith('.pat.gz') and op.isfile(
pat_path + '.csi'):
return mult_pat2beta(pat_path, out_beta, nr_sites, args)
cmd += ' {} | {} {} {}'.format(pat_path, PAT2BETA_TOOL, out_beta, nr_sites)
subprocess.check_call(cmd, shell=True)
return out_beta
def main():
"""
Run the WGBS pipeline to generate pat & beta files out of an input bam file
"""
parser = argparse.ArgumentParser(description=main.__doc__)
parser = add_args(parser)
args = parse_args(parser)
# validate output dir:
if not op.isdir(args.out_dir):
raise IllegalArgumentError(f'Invalid output dir: {args.out_dir}')
validate_local_exe(match_maker_tool)
validate_local_exe(patter_tool)
for bam in args.bam:
if not validate_bam(bam):
eprint(f'[wt bam2pat] Skipping {bam}')
continue
pat = op.join(args.out_dir, op.basename(bam)[:-4] + PAT_SUFF)
if not delete_or_skip(pat, args.force):
continue
Bam2Pat(args, bam)
def single_mix(self, rep):
mix_i = self.prefix + f'_{rep + 1}.pat.gz'
if not delete_or_skip(mix_i, self.args.force):
return
view_flags = []
for i in range(self.nr_pats):
v = ' '
if self.args.strict:
v += ' --strict'
if self.args.strip:
v += ' --strip'
if self.args.min_len:
v += f' --min_len {self.args.min_len}'
if self.args.bed_file is not None:
v += ' -L {}'.format(self.args.bed_file)
elif not self.gr.is_whole():
v += ' -s {}-{}'.format(*self.gr.sites)
v += ' --sub_sample {}'.format(self.adj_rates[i])
view_flags.append(v)
eprint('mix:', mix_i)
m = MergePats(self.pats, mix_i, self.labels, args=self.args)
m.fast_merge_pats(view_flags=view_flags)
def run_beta_to_bw(self, beta_path):
eprint('{}'.format(op.basename(beta_path)))
prefix = self.set_prefix(beta_path)
out_bigwig = prefix + BW_EXT
out_bed_graph = prefix + BG_EXT
cov_bigwig = prefix + COV_BW_EXT
cov_bed_graph = prefix + COV_BG_EXT
# Check if the current file should be skipped:
if not delete_or_skip(out_bigwig, self.args.force):
return
# load beta file
barr = self.load_beta(beta_path)
# dump coverage:
if self.args.dump_cov:
eprint('Dumping cov...')
self.ref_dict['cov'] = barr[:, 1]
sort_and_dump_df(
self.ref_dict[self.ref_dict['cov'] >= self.args.min_cov],
cov_bed_graph)
del self.ref_dict['cov']
# convert bedGraph to bigWig:
self.bed_graph_to_bigwig(cov_bed_graph, cov_bigwig)
# dump beta values to bedGraph
eprint('Dumping beta vals...')
self.ref_dict['beta'] = np.round(beta2vec(barr, na=-1), 3)
if self.args.remove_nan:
self.ref_dict = self.ref_dict[self.ref_dict['beta'] != -1]
sort_and_dump_df(self.ref_dict, out_bed_graph)
del self.ref_dict['beta']
# convert bedGraphs to bigWigs:
self.bed_graph_to_bigwig(out_bed_graph, out_bigwig)
def run_beta_to_bw(self, beta_path):
self.name = op.basename(beta_path)
prefix = op.join(self.outdir, op.splitext(self.name)[0])
out_bigwig = prefix + BW_EXT
out_bed_graph = prefix + BG_EXT
# Check if the current file should be skipped:
if not delete_or_skip(out_bigwig, self.args.force):
return
# convert beta to bed:
b2bw_log(f'[{self.name}] Dumping bed...')
beta_to_bed(beta_path=beta_path,
gr=self.gr,
bed_file=self.args.bed_file,
min_cov=self.args.min_cov,
mean=True,
keep_na=self.args.keep_na,
force=True,
opath=out_bed_graph)
# convert bedGraphs to bigWigs:
self.bed_graph_to_bigwig(out_bed_graph, out_bigwig)