def get_lapwiseerror_peranimal(self):
files = [f for f in os.listdir(self.BayesFolder)]
accuracy_dict = OrderedDict()
numlaps_dict = OrderedDict()
for f in files:
print(f)
animalname = f[:f.find('_')]
if animalname == 'CFC12' and self.CFC12flag == 0:
continue
animal_tasks = DataDetails.ExpAnimalDetails(
animalname)['task_dict']
trackbins = DataDetails.ExpAnimalDetails(animalname)['trackbins']
data = np.load(os.path.join(self.BayesFolder, f),
allow_pickle=True)
animal_accuracy = {k: [] for k in animal_tasks}
animal_numlaps = {k: [] for k in animal_tasks}
for t in animal_tasks:
animal_accuracy[t] = self.calulate_lapwiseerror(
y_actual=data['fit'].item()[t]['ytest'],
y_predicted=data['fit'].item()[t]['yang_pred'],
numlaps=data['numlaps'].item()[t],
lapframes=data['lapframes'].item()[t])
animal_numlaps[t] = data['numlaps'].item()[t]
accuracy_dict[animalname] = animal_accuracy
numlaps_dict[animalname] = animal_numlaps
return accuracy_dict, numlaps_dict
def compile_mean_bderror(self, ax):
mean_error = {k: [] for k in ['R2', 'Task', 'animalname', 'BD error (cm)', 'BD accuracy']}
for a in self.animals:
animalinfo = DataDetails.ExpAnimalDetails(a)
if self.datatype == 'endzonerem':
bayesmodel = np.load(
os.path.join(animalinfo['saveresults'], 'modeloneachtask_withendzonerem.npy'),
allow_pickle=True).item()
else:
bayesmodel = np.load(os.path.join(animalinfo['saveresults'], 'modeloneachtask.npy'),
allow_pickle=True).item()
for t in animalinfo['task_dict'].keys():
kfold = np.max(bayesmodel[t]['K-foldDataframe']['CVIndex'])
mean_error['R2'].extend(bayesmodel[t]['K-foldDataframe']['R2_angle'])
mean_error['BD accuracy'].extend(bayesmodel[t]['K-foldDataframe']['ModelAccuracy'])
for k in np.arange(kfold):
mean_error['BD error (cm)'].append(
np.mean(bayesmodel[t]['K-foldDataframe']['Y_ang_diff'][k] * self.trackbins))
mean_error['Task'].append(t)
mean_error['animalname'].append(a)
mean_error_df = pd.DataFrame.from_dict(mean_error)
for n, i in enumerate(['R2', 'BD accuracy', 'BD error (cm)']):
sns.boxplot(x='Task', y=i, data=mean_error_df, palette='Blues', ax=ax[n], showfliers=False)
for t in self.taskdict.keys():
if t != 'Task1':
d, p = Stats.significance_test(mean_error_df[mean_error_df.Task == t][i],
mean_error_df[mean_error_df.Task == 'Task1'][i],
type_of_test='KStest')
print(f'%s: %s: KStest: p-value %0.4f' % (i, t, p))
ax[n].set_xlabel('')
pf.set_axes_style(ax[n], numticks=4)
def __init__(self, DirectoryName, BayesFolder):
colors = sns.color_palette('muted')
self.colors = [colors[0], colors[1], colors[3], colors[2]]
self.Foldername = DirectoryName
self.BayesFolder = BayesFolder
self.SaveFolder = os.path.join(self.Foldername, 'SaveAnalysed')
self.animalname = [
f for f in os.listdir(self.Foldername)
if f not in ['LickData', 'BayesResults_All', 'SaveAnalysed']
]
print(self.animalname)
self.taskdict = ['Task1', 'Task2', 'Task3', 'Task4']
self.framespersec = 30.98
self.tracklength = 200
self.velocity_in_space, self.bayescompiled = OrderedDict(
), OrderedDict()
self.slope, self.speed_ratio = OrderedDict(), OrderedDict()
for a in self.animalname:
animalinfo = DataDetails.ExpAnimalDetails(a)
animaltasks = animalinfo['task_dict']
bayesfile = [f for f in os.listdir(self.BayesFolder) if a in f][0]
self.accuracy_dict = self.get_bayes_error(animaltasks, bayesfile)
self.goodrunningdata, self.running_data, self.good_running_index, self.lickdata, self.lapspeed, self.numlaps, = self.load_runningdata(
a)
self.good_lapframes = self.get_lapframes(a, animaltasks)
plt.plot(self.goodrunningdata['Task2'])
plt.plot(self.good_lapframes['Task2'])
plt.title(np.max(self.good_lapframes['Task2']))
plt.show()
self.velocity_in_space[a], self.bayescompiled[
a] = self.get_velocity_in_space_bylap(a, animaltasks)
self.slope[a], self.speed_ratio[a] = self.get_slopeatend(
a, animaltasks)
#
self.save_data()
def compile_confusion_matrix(self, fs, ax):
cm_all = {k: np.zeros((int(self.tracklength / self.trackbins), int(self.tracklength / self.trackbins))) for k in
self.taskdict.keys()}
for a in self.animals:
animalinfo = DataDetails.ExpAnimalDetails(a)
if self.datatype == 'endzonerem':
bayesmodel = np.load(
os.path.join(animalinfo['saveresults'], 'modeloneachtask_withendzonerem.npy'),
allow_pickle=True).item()
else:
bayesmodel = np.load(os.path.join(animalinfo['saveresults'], 'modeloneachtask.npy'),
allow_pickle=True).item()
for t in animalinfo['task_dict']:
cm = bayesmodel[t]['cm']
cm_all[t] += cm
for n, t in enumerate(self.taskdict.keys()):
cm_all[t] = cm_all[t].astype('float') / cm_all[t].sum(axis=1)[:, np.newaxis]
img = ax[n].imshow(cm_all[t], cmap="Blues", vmin=0, vmax=0.4, interpolation='nearest')
ax[n].plot(ax[n].get_xlim()[::-1], ax[n].get_ylim(), ls="--", c=".3", lw=1)
pf.set_axes_style(ax[n], numticks=3, both=True)
if n == len(self.taskdict) - 1:
CommonFunctions.create_colorbar(fighandle=fs, axis=ax[n], imghandle=img, title='Probability',
ticks=[0, 0.4])
ax[0].set_ylabel('Actual')
ax[0].set_xlabel('Predicted')
def get_data_peranimal(self):
for a in self.animalname:
print(a)
animalinfo = DataDetails.ExpAnimalDetails(a)
Fcdata, SmFcdata = self.gd.get_dff(a, animalinfo)
lapframes = self.gd.get_lapframes(a, animalinfo)
good_running_index, laps_with_licks, laps_without_licks = self.gd.get_behavior_params(
a)
placecell = self.gd.load_placecells(a)
placecell = placecell['Task1']
# activecells = self.get_cells_with_transients(animalinfo, Fcdata, SmFcdata)
auc, amplitude, length, frequency, numtransients = self.get_data_transients_pertask(
animalinfo,
placecell,
Fcdata,
SmFcdata,
lapframes=lapframes,
laps_withlicks=laps_with_licks,
laps_withoutlicks=laps_without_licks,
threshold=self.dffthreshold,
transthreshold=self.transthreshold)
self.save_transient_properties(a, auc, amplitude, length,
frequency, numtransients)
self.compile_to_dataframe(a, auc, amplitude, length, frequency,
numtransients)
def plot_compiled_errorcorrelation(axis, SaveFolder, TaskDict, trackbins, to_plot='R2', classifier_type='Bayes'):
l = Compile()
files = [f for f in os.listdir(SaveFolder) if classifier_type in f]
compilemeanerr = {k: [] for k in TaskDict.keys()}
compilelaptime = {k: [] for k in TaskDict.keys()}
for f in files:
print(f)
animalname = f[:f.find('_')]
animal_tasks = DataDetails.ExpAnimalDetails(animalname)['task_dict']
data = np.load(os.path.join(SaveFolder, f), allow_pickle=True)
for n, t in enumerate(animal_tasks):
m, laptime = l.plotlaptime_withbayeserror(axis=axis[n],
task=t,
lapframes=data['lapframes'].item()[t],
y_actual=data['fit'].item()[t]['ytest'],
y_predicted=data['fit'].item()[t]['yang_pred'],
numlaps=data['numlaps'].item()[t],
laptime=data['laptime'].item()[t],
trackbins=trackbins, to_plot=to_plot)
pf.set_axes_style(axis[n], numticks=4)
compilemeanerr[t].extend(m)
compilelaptime[t].extend(laptime)
# # get and plot best fit line
# for n, t in enumerate(TaskDict):
# regression_line = l.best_fit_slope_and_intercept(np.asarray(compilemeanerr[t]), np.asarray(compilelaptime[t]))
# corrcoef = np.corrcoef(np.asarray(compilemeanerr[t]), np.asarray(compilelaptime[t]))[0, 1]
# axis[n].plot(compilemeanerr[t], regression_line, color='k', linewidth=2)
# axis[n].set_title('%s r = %0.2f' % (t, corrcoef))
return compilelaptime, compilemeanerr
def plot_compiledconfusionmatrix(axis, SaveFolder, TaskDict, classifier_type='Bayes'):
files = [f for f in os.listdir(SaveFolder) if classifier_type in f]
all_ytest = {k: [] for k in TaskDict.keys()}
all_ypred = {k: [] for k in TaskDict.keys()}
for f in files:
animal_tasks = DataDetails.ExpAnimalDetails(f[:f.find('_')])['task_dict']
data = np.load(os.path.join(SaveFolder, f), allow_pickle=True)
print(f)
for n, t in enumerate(animal_tasks):
if t == 'Task1a':
all_ytest[t].extend(data['fit'].item()[t]['ytest'][-500:])
all_ypred[t].extend(data['fit'].item()[t]['yang_pred'][-500:])
else:
all_ytest[t].extend(data['fit'].item()[t]['ytest'])
all_ypred[t].extend(data['fit'].item()[t]['yang_pred'])
for n, t in enumerate(['Task1', 'Task1a']):
y_actual = all_ytest[t]
y_predicted = all_ypred[t]
cm = confusion_matrix(y_actual, y_predicted)
cm = cm.astype('float') / cm.sum(axis=1)[:, np.newaxis]
img = axis[n].imshow(cm, cmap="Blues", vmin=0, vmax=0.4, interpolation='nearest')
axis[n].plot(axis[n].get_xlim()[::-1], axis[n].get_ylim(), ls="--", c=".3", lw=1)
pf.set_axes_style(axis[n], numticks=3, both=True)
axis[n].set_title(t)
axis[0].set_ylabel('Actual')
axis[0].set_xlabel('Predicted')
def __init__(self, AnimalName, FolderName, CompiledFolderName,
classifier_type, taskstoplot):
print('Loading Data')
self.animalname = AnimalName
self.animalinfo = DataDetails.ExpAnimalDetails(self.animalname)
self.FolderName = os.path.join(FolderName, self.animalname)
self.CompiledFolderName = CompiledFolderName # For importing Bayes results
self.Task_Numframes = self.animalinfo['task_numframes']
self.TaskDict = self.animalinfo['task_dict']
self.classifier_type = classifier_type
self.framespersec = 30.98
self.taskstoplot = taskstoplot
self.trackbins = 5
# Run functions
self.get_data_folders()
if self.animalinfo['v73_flag']:
self.load_v73_Data()
else:
self.load_fluorescentdata()
self.get_place_cells()
self.load_behaviordata()
self.load_lapparams()
self.rasterdata = self.combinedata_forraster(self.taskstoplot)
self.colors = sns.color_palette('deep', len(self.taskstoplot))
def plot_histogram_error_bytask(SaveFolder, TaskDict, trackbins, to_plot='R2', classifier_type='Bayes'):
l = Compile()
files = [f for f in os.listdir(SaveFolder) if classifier_type in f]
axis_draw = {'Task1': 0, 'Task2': 0, 'Task2b': 0, 'Task3': 1, 'Task4': 2}
compileerror = {k: [] for k in TaskDict}
animalname = []
for f in files:
print(f)
animalname.append(f[:f.find('_')])
animal_tasks = DataDetails.ExpAnimalDetails(f[:f.find('_')])['task_dict']
data = np.load(os.path.join(SaveFolder, f), allow_pickle=True)
for n, t in enumerate(animal_tasks):
lap_r2, lap_accuracy = l.calulate_lapwiseerror(y_actual=data['fit'].item()[t]['ytest'],
y_predicted=data['fit'].item()[t]['yang_pred'],
trackbins=trackbins,
numlaps=data['numlaps'].item()[t],
lapframes=data['lapframes'].item()[t])
if to_plot == 'R2':
errdata = lap_r2[~np.isnan(lap_r2)]
else:
errdata = lap_accuracy[~np.isnan(lap_accuracy)]
if t == 'Task1':
compileerror[t].extend(errdata[-3:])
elif t == 'Task2':
lickstop = data['lickstoplap'].item()['Task2']
compileerror[t].extend(errdata[lickstop - 3:lickstop])
compileerror['Task2b'].extend(errdata[lickstop:lickstop + 3])
else:
compileerror[t].extend(errdata)
return compileerror
def combine_placecells_pertask(self, fig, axis, taskstoplot):
pc_activity_dict = {keys: np.asarray([]) for keys in taskstoplot}
perccells_peranimal = {keys: [] for keys in taskstoplot + ['animal']}
pcsortednum = {keys: [] for keys in taskstoplot}
for a in self.animals:
animalinfo = DataDetails.ExpAnimalDetails(a)
if len(animalinfo['task_dict']) == 4:
pf_remapping = np.load(os.path.join(self.FolderName, a,
'PlaceCells',
'%s_pcs_pertask.npy' % a),
allow_pickle=True).item()
pfparams = np.load(os.path.join(self.FolderName, a,
'PlaceCells',
f'%s_placecell_data.npz' % a),
allow_pickle=True)
perccells_peranimal['animal'].append(a)
for t in taskstoplot:
perccells_peranimal[t].append(
(np.sum(pfparams['numPFs_incells'].item()[t]) /
pfparams['numcells']) * 100)
# print(t, np.sum(pfparams['numPFs_incells'].item()[t]), np.shape(pf_remapping[t]))
pc_activity_dict[t] = np.vstack(
(pc_activity_dict[t], pf_remapping[t]
)) if pc_activity_dict[t].size else pf_remapping[t]
for t in taskstoplot:
print(t, np.shape(pc_activity_dict[t]))
pcsortednum[t] = np.argsort(np.nanargmax(pc_activity_dict[t], 1))
self.plpc.plot_placecells_pertask(fig, axis, taskstoplot,
pc_activity_dict, pcsortednum)
perccells_peranimal = pd.DataFrame.from_dict(perccells_peranimal)
perccells_peranimal = perccells_peranimal.set_index('animal')
return perccells_peranimal
def get_com_allanimal(self, taskA, taskB, vmax=0):
com_all_animal = np.array([])
count = 0
for n, f in enumerate(self.csvfiles_pfs):
animalname = f[:f.find('_')]
animalinfo = DataDetails.ExpAnimalDetails(animalname)
if len(animalinfo['task_dict']) >= self.tasklen:
df = pd.read_csv(os.path.join(self.CombinedDataFolder, f),
index_col=0)
t1 = df[df['Task'] == taskA]
t2 = df[df['Task'] == taskB]
combined = pd.merge(t1,
t2,
how='inner',
on=['CellNumber'],
suffixes=(f'_%s' % taskA, f'_%s' % taskB))
if count == 0:
com_all_animal = np.vstack(
(combined[f'WeightedCOM_%s' % taskA] * self.trackbins,
combined[f'WeightedCOM_%s' % taskB] * self.trackbins))
else:
com_all_animal = np.hstack(
(com_all_animal,
np.vstack((combined[f'WeightedCOM_%s' % taskA] *
self.trackbins,
combined[f'WeightedCOM_%s' % taskB] *
self.trackbins))))
count += 1
self.plot_com_scatter_heatmap(com_all_animal, taskA, taskB, vmax=vmax)
def __init__(self, AnimalName, FolderName, taskstoplot, controlflag=0):
print('Loading Data')
self.taskstoplot = taskstoplot
self.colors = sns.color_palette('deep', len(self.taskstoplot))
self.animalname = AnimalName
if controlflag:
self.animalinfo = DataDetails.ControlAnimals(self.animalname)
else:
self.animalinfo = DataDetails.ExpAnimalDetails(self.animalname)
self.FolderName = os.path.join(FolderName, self.animalname)
self.Task_Numframes = self.animalinfo['task_numframes']
self.TaskDict = self.animalinfo['task_dict']
# Run functions
self.get_data_folders()
self.load_behaviordata()
self.load_lapparams()
def combineanimaldataframes(self, csvfiles, tasklen):
count = 0
for n, f in enumerate(csvfiles):
animalname = f[:f.find('_')]
if not self.controlflag:
animalinfo = DataDetails.ExpAnimalDetails(animalname)
else:
animalinfo = DataDetails.ControlAnimals(animalname)
if len(animalinfo['task_dict']) >= tasklen:
print(f)
df = pd.read_csv(os.path.join(self.CombinedDataFolder, f),
index_col=0)
if count == 0:
combined_dataframe = df
else:
combined_dataframe = combined_dataframe.append(
df, ignore_index=True)
count += 1
return combined_dataframe
def plot_compilederror_withlick(axis,
SaveFolder,
TaskDict,
trackbins,
separate_lickflag=0,
licktype='all',
to_plot='R2',
classifier_type='Bayes'):
l = Compile()
files = [f for f in os.listdir(SaveFolder) if classifier_type in f]
compilelickdata = {k: [] for k in TaskDict.keys()}
compileerror = {k: [] for k in TaskDict.keys()}
lickstoplap = []
animalname = []
for f in files:
# print(f)
animalname.append(f[:f.find('_')])
animal_tasks = DataDetails.ExpAnimalDetails(
f[:f.find('_')])['task_dict']
print(len(animal_tasks))
data = np.load(os.path.join(SaveFolder, f), allow_pickle=True)
if data['lickstoplap'].item()['Task2'] > 2:
print(f)
for n, t in enumerate(animal_tasks):
lap_r2, lap_accuracy = l.calulate_lapwiseerror(
y_actual=data['fit'].item()[t]['ytest'],
y_predicted=data['fit'].item()[t]['yang_pred'],
trackbins=trackbins,
numlaps=data['numlaps'].item()[t],
lapframes=data['lapframes'].item()[t])
if licktype == 'all':
licks = data['alllicks'].item()
lickstop = data['lickstoplap'].item()['Task2']
else:
licks = data['licks_befclick'].item()
lickstop = data['lickstoplap_befclick'].item()['Task2']
if to_plot == 'R2':
data_compile = lap_r2
else:
data_compile = lap_accuracy
l.plot_bayeserror_with_lickrate(
data_compile, licks[t],
data['lickstoplap'].item()['Task2'], t, axis[n],
separate_lickflag)
pf.set_axes_style(axis[n], numticks=4)
compilelickdata[t].append(licks[t])
compileerror[t].append(np.asarray(data_compile))
lickstoplap.append(lickstop)
return compileerror, compilelickdata, np.asarray(lickstoplap), animalname
def __init__(self, AnimalName, FolderName, SaveFigureFolder, taskstoplot, controlflag=0):
print('Loading Data')
self.taskstoplot = taskstoplot
self.SaveFigureFolder = SaveFigureFolder
self.controlflag = controlflag
if self.controlflag:
self.colors = sns.color_palette(["#3498db", "#9b59b6"])
else:
self.colors = sns.color_palette('deep')
self.task2_colors = [self.colors[1], self.colors[3]]
self.animalname = AnimalName
if self.controlflag:
self.animalinfo = DataDetails.ControlAnimals(self.animalname)
else:
self.animalinfo = DataDetails.ExpAnimalDetails(self.animalname)
self.ParentFolderName = FolderName
self.FolderName = os.path.join(FolderName, self.animalname)
self.Task_Numframes = self.animalinfo['task_numframes']
self.removeframesforbayes = self.animalinfo['task_framestokeep']
self.TaskDict = self.animalinfo['task_dict']
self.framespersec = 30.98
self.trackbins = 5
# Run functions
self.get_data_folders()
if self.animalinfo['v73_flag']:
self.load_v73_Data()
else:
self.load_fluorescentdata()
self.load_Bayesfit()
self.load_behaviordata()
self.load_lapparams()
if not self.controlflag:
self.lickstoplap = np.int(self.lickstoplap['Task2'] - 1)
self.lickstopframe = np.where(self.good_lapframes['Task2'] == self.lickstoplap)[0][0]
def __init__(self, FolderName, animalname):
self.FolderName = FolderName
self.animalname = animalname
self.animalinfo = DataDetails.ExpAnimalDetails(self.animalname)
self.Task_Numframes = self.animalinfo['task_numframes']
self.TaskDict = self.animalinfo['task_dict']
self.framespersec = 30.98
self.trackbins = 5
self.tracklength = 200
self.trackstartindex = self.animalinfo['trackstart_index']
self.get_fluorescence_data()
self.get_behavior()
self.load_lapparams()
self.calculate_velocity()
def compile_meanerror_bytrack(self, ax):
numbins = int(self.tracklength / self.trackbins)
numanimals = np.size(self.animals)
kfold = 10
Y_diff_by_track = {k: np.zeros((numanimals, kfold, numbins)) for k in ['Task1', 'Task2']}
Y_diff_by_track_mean = {k: [] for k in ['Task1', 'Task2']}
for n, a in enumerate(self.animals):
print(a)
animalinfo = DataDetails.ExpAnimalDetails(a)
if self.datatype == 'endzonerem':
bayesmodel = np.load(
os.path.join(animalinfo['saveresults'], 'modeloneachtask_withendzonerem.npy'),
allow_pickle=True).item()
else:
bayesmodel = np.load(os.path.join(animalinfo['saveresults'], 'modeloneachtask.npy'),
allow_pickle=True).item()
for t in ['Task1', 'Task2']:
for k in np.arange(kfold):
y_diff = np.asarray(bayesmodel[t]['K-foldDataframe']['Y_ang_diff'][k]) * self.trackbins
y_test = np.asarray(bayesmodel[t]['K-foldDataframe']['y_test'][k])
for i in np.arange(numbins):
Y_indices = np.where(y_test == i)[0]
Y_diff_by_track[t][n, k, i] = np.mean(y_diff[Y_indices])
for t in ['Task1', 'Task2']:
Y_diff_by_track_mean[t] = Y_diff_by_track[t].reshape(numanimals * kfold, numbins)
meandiff, semdiff = np.nanmean(Y_diff_by_track_mean[t], 0), scipy.stats.sem(Y_diff_by_track_mean[t], 0,
nan_policy='omit')
error1, error2 = meandiff - semdiff, meandiff + semdiff
ax.plot(np.arange(numbins), meandiff)
ax.fill_between(np.arange(numbins), error1, error2, alpha=0.5)
ax.set_xlabel('Track Length (cm)')
ax.set_ylabel('BD error (cm)')
ax.set_xlim((0, numbins))
d, p = Stats.significance_test(np.mean(Y_diff_by_track_mean['Task2'], 0),
np.mean(Y_diff_by_track_mean['Task1'], 0),
type_of_test='KStest')
print(f'KStest: p-value %0.4f' % p)
CommonFunctions.convertaxis_to_tracklength(ax, self.tracklength, self.trackbins, convert_axis='x')
ax.set_xlim((0, self.tracklength / self.trackbins))
pf.set_axes_style(ax, numticks=4)
def get_com_allanimal(self, fig, axis, taskA, taskB, vmax=0):
csvfiles_pfs = [
f for f in os.listdir(self.CombinedDataFolder)
if f.endswith('.csv') and 'reward' not in f and 'common' not in f
]
com_all_animal = np.array([])
count = 0
for n, f in enumerate(csvfiles_pfs):
a = f[:f.find('_')]
animalinfo = DataDetails.ExpAnimalDetails(a)
if len(animalinfo['task_dict']) == 4:
print(f)
df = pd.read_csv(os.path.join(self.CombinedDataFolder, f),
index_col=0)
t1 = df[df['Task'] == taskA]
t2 = df[df['Task'] == taskB]
combined = pd.merge(t1,
t2,
how='inner',
on=['CellNumber'],
suffixes=(f'_%s' % taskA, f'_%s' % taskB))
if count == 0:
com_all_animal = np.vstack(
(combined[f'WeightedCOM_%s' % taskA] * self.trackbins,
combined[f'WeightedCOM_%s' % taskB] * self.trackbins))
else:
com_all_animal = np.hstack(
(com_all_animal,
np.vstack((combined[f'WeightedCOM_%s' % taskA] *
self.trackbins,
combined[f'WeightedCOM_%s' % taskB] *
self.trackbins))))
count += 1
self.plpc.plot_com_scatter_heatmap(fig,
axis,
com_all_animal,
taskA,
taskB,
self.tracklength,
vmax=vmax)
return np.abs(np.subtract(com_all_animal[0, :], com_all_animal[1, :]))
def __init__(self, AnimalName, FolderName, SaveFigureFolder, taskstoplot):
self.taskstoplot = taskstoplot
self.SaveFigureFolder = SaveFigureFolder
self.colors = sns.color_palette('deep')
self.task2_colors = [self.colors[1], self.colors[3]]
self.animalname = AnimalName
self.animalinfo = DataDetails.ExpAnimalDetails(self.animalname)
self.ParentFolderName = FolderName
self.FolderName = os.path.join(FolderName, self.animalname)
self.Task_Numframes = self.animalinfo['task_numframes']
self.Task_Numframes['Task3'] = 14999
self.removeframesforbayes = self.animalinfo['task_framestokeep']
self.TaskDict = self.animalinfo['task_dict']
self.framespersec = 30.98
self.trackbins = 5
self.get_data_folders()
self.load_lapparams()
self.load_behaviordata()
def compile_numcells(self, ax, taskstoplot, placecellflag=0):
percsamples = [5, 10, 20, 50, 80, 100]
percsamples = [f'%d%%' % p for p in percsamples]
numcells_combined = pd.DataFrame([])
for a in self.animals:
animalinfo = DataDetails.ExpAnimalDetails(a)
bayesmodel = np.load(os.path.join(animalinfo['saveresults'],
'modeloneachtask_lapwise.npy'),
allow_pickle=True).item()
for t in animalinfo['task_dict']:
if not placecellflag:
numcells_dataframe = bayesmodel[t]['Numcells_Dataframe']
else:
numcells_dataframe = bayesmodel[t][
'Placecells_sample_Dataframe']
numcells_dataframe['Task'] = t
numcells_dataframe['animalname'] = a
numcells_combined = pd.concat(
(numcells_combined, numcells_dataframe), ignore_index=True)
g = numcells_combined.groupby(['SampleSize', 'Task', 'animalname'
]).agg([np.mean]).reset_index()
g.columns = g.columns.droplevel(1)
if placecellflag:
g['Type'] = 'Placecells'
else:
g['Type'] = 'Allcells'
sns.pointplot(x='SampleSize',
y='R2_angle',
data=g[g.Task.isin(taskstoplot)],
order=percsamples,
hue='Task',
ax=ax)
ax.legend(loc='center left', bbox_to_anchor=(1, 0.5))
ax.set_xlabel('Percentage of active cells used')
ax.set_ylabel('R-squared')
pf.set_axes_style(ax, numticks=4)
return g
def combine_placecells_withtask(self,
fig,
axis,
taskstoplot,
tasktocompare='Task1'):
pc_activity_dict = {keys: np.asarray([]) for keys in taskstoplot}
for a in self.animals:
animalinfo = DataDetails.ExpAnimalDetails(a)
if len(animalinfo['task_dict']) == 4:
pf_remapping = np.load(os.path.join(
self.FolderName, a, 'PlaceCells',
'%s_pcs_sortedbyTask1.npy' % a),
allow_pickle=True).item()
for t in taskstoplot:
pc_activity_dict[t] = np.vstack(
(pc_activity_dict[t], pf_remapping[t]
)) if pc_activity_dict[t].size else pf_remapping[t]
pcsortednum = {keys: [] for keys in taskstoplot}
pcsorted = np.argsort(np.nanargmax(pc_activity_dict[tasktocompare], 1))
for t in taskstoplot:
pcsortednum[t] = pcsorted
# Correlate place cells
corrcoef_dict = self.find_correlation(pc_activity_dict, taskstoplot,
tasktocompare)
task_data = pc_activity_dict['Task1'][pcsorted, :]
normalise_data = np.nanmax(task_data, 1)[:, np.newaxis]
self.plpc.plot_placecells_pertask(fig,
axis,
taskstoplot,
pc_activity_dict,
pcsortednum,
normalise_data=normalise_data)
return corrcoef_dict, pc_activity_dict, pcsortednum
def __init__(self, AnimalName, FolderName, SaveFigureFolder, taskstoplot):
self.taskstoplot = taskstoplot
self.SaveFigureFolder = SaveFigureFolder
self.colors = sns.color_palette('deep')
self.task2_colors = [self.colors[1], self.colors[3]]
self.animalname = AnimalName
self.animalinfo = DataDetails.ExpAnimalDetails(self.animalname)
self.ParentFolderName = FolderName
self.FolderName = os.path.join(FolderName, self.animalname)
self.Task_Numframes = self.animalinfo['task_numframes']
self.removeframesforbayes = self.animalinfo['task_framestokeep']
self.TaskDict = self.animalinfo['task_dict']
self.framespersec = 30.98
self.trackbins = 5
self.get_data_folders()
if self.animalinfo['v73_flag']:
self.load_v73_Data()
else:
self.load_fluorescentdata()
self.load_lapparams()
self.load_behaviordata()
self.raster_fdata, self.raster_cdata = self.combinedata_correct_forraster(
)
self.make_rastermap(self.raster_fdata, self.raster_cdata)
fs, ax = plt.subplots(3,
sharex='all',
dpi=300,
gridspec_kw={
'height_ratios': [2, 0.5, 0.5],
'hspace': 0.3
})
self.plot_rastermap(ax,
fdata=self.raster_fdata,
crop_cellflag=0,
ylim_meandff=0.1)
def compile_numcells(self, ax, taskstoplot, legendflag=0):
percsamples = [1, 5, 10, 20, 50, 80, 100]
percsamples = [f'%d%%' % p for p in percsamples]
numcells_combined = pd.DataFrame([])
for a in self.animals:
print(a)
animalinfo = DataDetails.ExpAnimalDetails(a)
if self.datatype == 'endzonerem':
bayesmodel = np.load(
os.path.join(animalinfo['saveresults'], 'modeloneachtask_withendzonerem.npy'),
allow_pickle=True).item()
else:
bayesmodel = np.load(os.path.join(animalinfo['saveresults'], 'modeloneachtask.npy'),
allow_pickle=True).item()
for t in animalinfo['task_dict']:
numcells_dataframe = bayesmodel[t]['Numcells_Dataframe']
numcells_dataframe['Task'] = t
numcells_dataframe['animalname'] = a
numcells_combined = pd.concat((numcells_combined, numcells_dataframe), ignore_index=True)
g = numcells_combined.groupby(['SampleSize', 'Task', 'animalname']).agg([np.mean]).reset_index()
g.columns = g.columns.droplevel(1)
sns.pointplot(x='SampleSize', y='R2_angle', data=g[g.Task.isin(taskstoplot)], order=percsamples, hue='Task',
ax=ax)
if legendflag:
ax.legend(loc='center left', bbox_to_anchor=(1, 0.5))
else:
ax.get_legend().remove()
ax.set_xlabel('Percentage of active cells used')
ax.set_ylabel('R-squared')
# ax.set_aspect(aspect=1.6)
pf.set_axes_style(ax, numticks=4)
for t in self.taskdict:
if t != 'Task1':
d, p = Stats.significance_test(g[g.Task == t]['R2'], g[g.Task == 'Task1']['R2'], type_of_test='KStest')
print(f'%s: KStest : p-value %0.4f' % (t, p))
def get_lapwise_correlation_peranimal(self, taskstoplot, axis):
numlaps = {'Task1': 5, 'Task2': 14, 'Task3': 11}
correlation_data = np.zeros((len(self.npyfiles) - 2, sum(numlaps.values())))
lick_data = np.zeros((len(self.npyfiles) - 2, sum(numlaps.values())))
count = 0
for n1, f in enumerate(self.npyfiles):
print(f, count, np.shape(correlation_data))
animalname = f[: f.find('_')]
animal_tasks = DataDetails.ExpAnimalDetails(animalname)['task_dict']
corr_data = self.get_correlation_data(f)
corr_animal = corr_data['correlation_withTask1'].item()
sigPFs = corr_data['sig_PFs_cellnum'].item()['Task1']
lickstoplap = self.get_animal_behaviordata(animalname)['lick_stop'].item()['Task2']
lick_per_lap = self.get_animal_behaviordata(animalname)['numlicks_withinreward_alllicks'].item()
if lickstoplap > 2:
count_lap = 0
for n2, t in enumerate(animal_tasks.keys()):
if t in taskstoplot:
corr_sigPFs = corr_animal[t][sigPFs, :]
tasklap = np.size(corr_animal[t], 1)
if t == 'Task1':
randlaps = np.random.choice(np.arange(0, tasklap), numlaps[t], replace=False)
this_task_data = np.nanmedian(corr_sigPFs[:, np.arange(12, 12 + numlaps[t])], 0)
this_lick_data = lick_per_lap[t][-numlaps[t]:]
elif t == 'Task2':
this_task_data = np.nanmedian(corr_sigPFs[:, lickstoplap - 3:lickstoplap + 11], 0)
this_lick_data = lick_per_lap[t][lickstoplap - 3:lickstoplap + 11]
else:
this_task_data = np.nanmedian(corr_sigPFs[:, :numlaps[t]], 0)
this_lick_data = lick_per_lap[t][:numlaps[t]]
correlation_data[count, count_lap:count_lap + numlaps[t]] = this_task_data
lick_data[count, count_lap:count_lap + numlaps[t]] = this_lick_data
count_lap += numlaps[t]
count += 1
# Normalize and compare for p-value with Task1
corr_norm = correlation_data / np.max(correlation_data[:, :numlaps['Task1']])
lick_norm = lick_data / np.max(lick_data[:, :numlaps['Task1']])
# Plot_traces
plot_axis = [axis, axis.twinx()]
colors = sns.color_palette('dark', 2)
label = ['Mean Correlation', 'Mean Licks']
for n, d in enumerate([corr_norm, lick_norm]):
mean = np.mean(d, 0)
sem = scipy.stats.sem(d, 0)
if n == 0:
plot_axis[n].errorbar(np.arange(np.size(mean)), mean, yerr=sem, color=colors[n])
plot_axis[n].plot(np.arange(np.size(mean)), mean, '.-', color=colors[n])
plot_axis[n].set_ylabel(label[n], color=colors[n])
# Get p-values
for l in np.arange(np.size(correlation_data, 1)):
d, p = scipy.stats.ranksums(correlation_data[:, l], correlation_data[:, 0])
if np.round(p, 3) < 0.05:
axis.plot(l, 0.9, '*', color='k')
print(l, p)
for a in plot_axis:
pf.set_axes_style(axis)
axis.set_xlabel('Lap Number')
return correlation_data, lick_data
def plot_lapwiseerror_withlick(axis,
SaveFolder,
taskstoplot,
trackbins=5,
to_plot='R2',
classifier_type='Bayes'):
numlaps = {'Task1': 5, 'Task2': 14, 'Task3': 11}
l = Compile()
files = [f for f in os.listdir(SaveFolder) if classifier_type in f]
correlation_data = np.zeros((len(files) - 2, sum(numlaps.values())))
lick_data = np.zeros((len(files) - 2, sum(numlaps.values())))
count = 0
for n1, f in enumerate(files):
animalname = f[:f.find('_')]
animal_tasks = DataDetails.ExpAnimalDetails(animalname)['task_dict']
data = np.load(os.path.join(SaveFolder, f), allow_pickle=True)
lickstoplap = data['lickstoplap'].item()['Task2']
lick_per_lap = data['alllicks'].item()
if lickstoplap > 2:
print(f)
count_lap = 0
for t in animal_tasks.keys():
if t in taskstoplot:
lap_r2, lap_accuracy = l.calulate_lapwiseerror(
y_actual=data['fit'].item()[t]['ytest'],
y_predicted=data['fit'].item()[t]['yang_pred'],
trackbins=trackbins,
numlaps=data['numlaps'].item()[t],
lapframes=data['lapframes'].item()[t])
if to_plot == 'R2':
decodererror = np.asarray(lap_r2)
else:
decodererror = np.asarray(lap_accuracy)
decodererror = decodererror[~np.isnan(decodererror)]
if t == 'Task1':
this_task_data = decodererror[-numlaps[t]:]
this_lick_data = lick_per_lap[t][-numlaps[t]:]
elif t == 'Task2':
this_task_data = decodererror[lickstoplap -
3:lickstoplap + 11]
this_lick_data = lick_per_lap[t][lickstoplap -
3:lickstoplap + 11]
else:
this_task_data = decodererror[:numlaps[t]]
this_lick_data = lick_per_lap[t][:numlaps[t]]
correlation_data[count, count_lap:count_lap +
numlaps[t]] = this_task_data
lick_data[count, count_lap:count_lap +
numlaps[t]] = this_lick_data
count_lap += numlaps[t]
count += 1
# Normalize and compare for p-value with Task1
corr_norm = correlation_data / np.max(
correlation_data[:, :numlaps['Task1']])
lick_norm = lick_data / np.max(lick_data[:, :numlaps['Task1']])
# Plot_traces
plot_axis = [axis, axis.twinx()]
color_animal = sns.color_palette('deep', len(taskstoplot))
color_data = sns.color_palette('dark', 2)
if to_plot == 'R2':
label = ['Mean R-squared', 'Mean Licks']
else:
label = ['Mean Accuracy', 'Mean Licks']
for n, d in enumerate([corr_norm, lick_norm]):
mean = np.mean(d, 0)
sem = scipy.stats.sem(d, 0)
count = 0
for n2, l1 in enumerate(taskstoplot):
data_m = mean[count:count + numlaps[l1]]
data_sem = sem[count:count + numlaps[l1]]
if n == 0:
plot_axis[n].errorbar(np.arange(count, count + numlaps[l1]),
data_m,
yerr=data_sem,
color=color_animal[n2])
else:
plot_axis[n].plot(np.arange(np.size(mean)),
mean,
'.-',
color=color_data[n],
zorder=n)
plot_axis[n].set_ylabel(label[n], color=color_data[n])
count += numlaps[l1]
plot_axis[0].set_ylim((0, 1))
plot_axis[1].set_ylim((0, 1))
# Get p-values
for l in np.arange(np.size(correlation_data, 1)):
d, p = scipy.stats.ranksums(correlation_data[:, l],
correlation_data[:, 0])
if np.round(p, 3) < 0.01:
if to_plot == 'R2':
axis.plot(l, 1.0, '*', color='k')
else:
axis.plot(l, 1.5, '*', color='k')
print(l, p)
for a in plot_axis:
pf.set_axes_style(axis)
axis.set_xlabel('Lap Number')
return correlation_data, lick_data
def plot_meancorrelation_withshuffle(axis,
SaveFolder,
trackbins,
taskstoplot,
classifier_type='Bayes',
to_plot='R2'):
# Choose last 10 laps in Task1, random 4 laps in Task2, and Task2b 100 times to calculate mean decoding error per animal
num_iterations = 1000
l = Compile()
colors = sns.color_palette('deep')
colors = [colors[0], colors[1], colors[3], colors[2]]
files = [f for f in os.listdir(SaveFolder) if classifier_type in f]
shuffle_mean_corr = {
k: np.zeros((num_iterations, len(files) - 2))
for k in taskstoplot
}
count = 0
for n, f in enumerate(files):
animalname = f[:f.find('_')]
animal_tasks = DataDetails.ExpAnimalDetails(
f[:f.find('_')])['task_dict']
data = np.load(os.path.join(SaveFolder, f), allow_pickle=True)
lickstoplap = data['lickstoplap'].item()['Task2']
if data['lickstoplap'].item()['Task2'] > 2:
print(f)
for t in animal_tasks:
if t in taskstoplot:
lap_r2, lap_accuracy = l.calulate_lapwiseerror(
y_actual=data['fit'].item()[t]['ytest'],
y_predicted=data['fit'].item()[t]['yang_pred'],
trackbins=trackbins,
numlaps=data['numlaps'].item()[t],
lapframes=data['lapframes'].item()[t])
if to_plot == 'R2':
decodererror = np.asarray(lap_r2)
else:
decodererror = np.asarray(lap_accuracy)
decodererror = decodererror[~np.isnan(decodererror)]
tasklap = np.size(decodererror)
for i in np.arange(num_iterations):
if t == 'Task2':
randlaps = np.random.choice(np.arange(
0, lickstoplap),
4,
replace=False)
shuffle_mean_corr[t][i, count] = np.nanmean(
decodererror[randlaps])
randlaps = np.random.choice(np.arange(
lickstoplap, tasklap),
4,
replace=False)
shuffle_mean_corr['Task2b'][i, count] = np.nanmean(
decodererror[randlaps])
if np.any(
np.isnan(np.nanmean(
decodererror[randlaps]))):
print(animalname, t, randlaps, decodererror)
elif t == 'Task1':
randlaps = np.random.choice(np.arange(
tasklap - 5, tasklap),
4,
replace=False)
shuffle_mean_corr[t][i, count] = np.nanmean(
decodererror[randlaps])
else:
randlaps = np.random.choice(np.arange(0, tasklap),
4,
replace=False)
shuffle_mean_corr[t][i, count] = np.nanmean(
decodererror[randlaps])
count += 1
# Get p-value
p_value_task2 = []
p_value_task2b = []
for i in np.arange(num_iterations):
t, p = scipy.stats.ttest_rel(shuffle_mean_corr['Task1'][i, :],
shuffle_mean_corr['Task2'][i, :])
p_value_task2.append(p > 0.05)
t, p = scipy.stats.ttest_rel(shuffle_mean_corr['Task1'][i, :],
shuffle_mean_corr['Task2b'][i, :])
p_value_task2b.append(p > 0.05)
# Plot shuffle histogram
# Remove zeros
data = {k: [] for k in ['Task1', 'Task2', 'Task2b']}
for n, t in enumerate(['Task1', 'Task2', 'Task2b']):
temp = shuffle_mean_corr[t].flatten()
data[t] = temp
if to_plot == 'R2':
sns.distplot(data[t],
label=t,
bins=np.linspace(0, 1, 100),
ax=axis[1, 0],
hist_kws={'color': colors[n]},
kde_kws={'color': colors[n]})
else:
sns.distplot(data[t],
label=t,
bins=np.linspace(0, 50, 50),
ax=axis[1, 0],
hist_kws={'color': colors[n]},
kde_kws={'color': colors[n]})
axis[1, 0].set_title(
'Shuffled laps P-value with lick %0.3f, without lick %0.3f' %
(np.size(np.where(p_value_task2)) / num_iterations,
np.size(np.where(p_value_task2b)) / num_iterations))
axis[1, 0].legend(loc='center left', bbox_to_anchor=(1, 0.5))
axis[1, 0].set_xlabel('R-squared')
axis[1, 0].set_xlim((-0.1, 1.0))
axis[1, 0].legend(loc='center left', bbox_to_anchor=(1, 0.5))
t, p1 = scipy.stats.ks_2samp(data['Task1'], data['Task2'])
t, p2 = scipy.stats.ks_2samp(data['Task1'], data['Task2b'])
print('Flattened P-value with lick %f, without lick %f' % (p1, p2))
# Get mean_correlation
mean_correlation = {k: [] for k in taskstoplot}
sem_correlation = {k: [] for k in taskstoplot}
for t in taskstoplot:
mean_correlation[t] = np.mean(shuffle_mean_corr[t], 0)
sem_correlation[t] = scipy.stats.sem(shuffle_mean_corr[t],
0,
nan_policy='omit')
df = pd.DataFrame.from_dict(mean_correlation)
df = df.replace(0, np.nan)
df = df.dropna(how='all')
for p in np.arange(2):
if p == 0:
df_melt = df.melt(var_name='Task', value_name='Error')
for index, row in df.iterrows():
axis[0, p].plot(
[row['Task1'], row['Task2'], row['Task2b'], row['Task3']],
'k')
print(df)
else:
df_div = df[df.columns].div(df['Task1'].values, axis=0)
print(df_div)
df_melt = df_div.melt(var_name='Task', value_name='Error')
for index, row in df_div.iterrows():
axis[0, p].plot(
[row['Task1'], row['Task2'], row['Task2b'], row['Task3']],
'k')
sns.boxplot(x='Task',
y='Error',
data=df_melt,
palette=colors,
order=['Task1', 'Task2', 'Task2b', 'Task3'],
ax=axis[0, p])
sns.stripplot(x='Task',
y='Error',
data=df_melt,
color='k',
size=5,
order=['Task1', 'Task2', 'Task2b', 'Task3'],
ax=axis[0, p],
dodge=False,
jitter=False)
axis[0, p].set_xlabel('')
axis[0, p].set_ylim((0, 1.1))
t, p1 = scipy.stats.ttest_rel(df['Task1'], df['Task2'])
t, p2 = scipy.stats.ks_2samp(df['Task1'], df['Task2b'])
print('Mean P-value with lick %f, without lick %f' % (p1, p2))
axis[1, 1].axis('off')
for a in axis.flatten():
pf.set_axes_style(a, numticks=4)
return shuffle_mean_corr
def get_mean_correlation_withshuffle(self, axis, taskstoplot):
num_iterations = 1000
shuffle_mean_corr = {k: np.zeros((num_iterations, np.size(self.npyfiles) - 2)) for k in taskstoplot}
count = 0
for n, f in enumerate(self.npyfiles):
animalname = f[: f.find('_')]
animal_tasks = DataDetails.ExpAnimalDetails(animalname)['task_dict']
corr_data = self.get_correlation_data(f)
corr_animal = corr_data['correlation_withTask1'].item()
sigPFs = corr_data['sig_PFs_cellnum'].item()['Task1']
lickstoplap = self.get_animal_behaviordata(animalname)['lick_stop'].item()['Task2']
if lickstoplap > 2:
for t in animal_tasks.keys():
if t in taskstoplot:
tasklap = np.size(corr_animal[t], 1)
corr_data_pfs = corr_animal[t][sigPFs, :]
for i in np.arange(num_iterations):
if t == 'Task2':
randlaps = np.random.choice(np.arange(0, lickstoplap), 4, replace=False)
shuffle_mean_corr[t][i, count] = np.mean(corr_data_pfs[:, randlaps].flatten())
randlaps = np.random.choice(np.arange(lickstoplap, tasklap), 4, replace=False)
shuffle_mean_corr['Task2b'][i, count] = np.mean(
corr_data_pfs[:, randlaps].flatten())
else:
randlaps = np.random.choice(np.arange(0, tasklap - 5), 4, replace=False)
shuffle_mean_corr[t][i, count] = np.mean(corr_data_pfs[:, randlaps].flatten())
count += 1
# Get p-value
p_value_task2 = []
p_value_task2b = []
for i in np.arange(num_iterations):
t, p = scipy.stats.ttest_rel(shuffle_mean_corr['Task1'][i, :], shuffle_mean_corr['Task2'][i, :])
p_value_task2.append(p > 0.01)
t, p = scipy.stats.ttest_rel(shuffle_mean_corr['Task1'][i, :], shuffle_mean_corr['Task2b'][i, :])
p_value_task2b.append(p > 0.01)
print('Shuffled laps P-value with lick %0.3f, without lick %0.3f' % (
np.size(np.where(p_value_task2)) / num_iterations, np.size(np.where(p_value_task2b)) / num_iterations))
# Plot shuffle histogram
# Remove zeros
data = {k: [] for k in ['Task1', 'Task2', 'Task2b']}
for t in ['Task1', 'Task2', 'Task2b']:
temp = shuffle_mean_corr[t].flatten()
data[t] = temp
sns.distplot(data[t], label=t,
bins=np.linspace(0, 1, 50), ax=axis[1, 0])
axis[1, 0].legend(loc='center left', bbox_to_anchor=(1, 0.5))
t, p1 = scipy.stats.ks_2samp(data['Task1'], data['Task2'])
t, p2 = scipy.stats.ks_2samp(data['Task1'], data['Task2b'])
print('Flattened P-value with lick %f, without lick %f' % (p1, p2))
# Get mean_correlation
mean_correlation = {k: [] for k in taskstoplot}
sem_correlation = {k: [] for k in taskstoplot}
for t in taskstoplot:
mean_correlation[t] = np.mean(shuffle_mean_corr[t], 0)
sem_correlation[t] = scipy.stats.sem(shuffle_mean_corr[t], 0, nan_policy='omit')
df = pd.DataFrame.from_dict(mean_correlation)
df = df.replace(0, np.nan)
df = df.dropna(how='all')
for p in np.arange(2):
if p == 0:
df_melt = df.melt(var_name='Task', value_name='Error')
for index, row in df.iterrows():
axis[0, p].plot([row['Task1'], row['Task2'], row['Task2b'], row['Task3']], 'k')
print(df)
else:
df_div = df[df.columns].div(df['Task1'].values, axis=0)
print(df_div)
df_melt = df_div.melt(var_name='Task', value_name='Error')
for index, row in df_div.iterrows():
axis[0, p].plot([row['Task1'], row['Task2'], row['Task2b'], row['Task3']], 'k')
sns.boxplot(x='Task', y='Error', data=df_melt, palette='Blues', order=[
'Task1', 'Task2', 'Task2b', 'Task3'], ax=axis[0, p])
sns.stripplot(x='Task', y='Error', data=df_melt, color='k', size=5, order=[
'Task1', 'Task2', 'Task2b', 'Task3'], ax=axis[0, p], dodge=False, jitter=False)
axis[0, p].set_xlabel('')
t, p1 = scipy.stats.ttest_rel(df['Task1'], df['Task2'])
t, p2 = scipy.stats.ks_2samp(df['Task1'], df['Task2b'])
print('Mean P-value with lick %f, without lick %f' % (p1, p2))
axis[1, 1].axis('off')
for a in axis.flatten():
pf.set_axes_style(a)
return shuffle_mean_corr, mean_correlation, data
def compile_meanerror_bytrack_incontrols(self, ControlFolder, ax):
numbins = int(self.tracklength / self.trackbins)
animals = [
f for f in os.listdir(ControlFolder)
if f not in ['BayesResults_All', 'SaveAnalysed']
]
Y_diff_by_track = {k: [] for k in ['Task1a', 'Task1b']}
for n, a in enumerate(animals):
animalinfo = DataDetails.ControlAnimals(a)
bayesmodel = np.load(os.path.join(animalinfo['saveresults'],
'modeloneachtask.npy'),
allow_pickle=True).item()
for t in animalinfo['task_dict']:
kfold = np.size(bayesmodel[t]['K-foldDataframe']['CVIndex'])
for k in np.arange(6):
y_predict = np.asarray(
bayesmodel[t]['K-foldDataframe']['y_predict_angle'][k])
y_test = np.asarray(
bayesmodel[t]['K-foldDataframe']['y_test'][k])
y_diff = np.abs(
np.nan_to_num(y_predict) -
np.nan_to_num(y_test)) * self.trackbins
y_diff_append = np.zeros(numbins)
for i in np.arange(numbins):
Y_indices = np.where(y_test == i)[0]
y_diff_append[i] = np.nanmean(y_diff[Y_indices])
Y_diff_by_track[t].append(y_diff_append)
for t in ['Task1a', 'Task1b']:
Y_diff_by_track[t] = np.asarray(Y_diff_by_track[t])
Y_diff_by_animal = np.abs(Y_diff_by_track['Task1a'] -
Y_diff_by_track['Task1b'])
for t in ['Task1a', 'Task1b']:
meandiff, semdiff = np.nanmean(
Y_diff_by_track[t], 0), scipy.stats.sem(Y_diff_by_track[t],
0,
nan_policy='omit')
error1, error2 = meandiff - semdiff, meandiff + semdiff
ax[0].plot(np.arange(numbins), meandiff)
ax[0].fill_between(np.arange(numbins), error1, error2, alpha=0.5)
ax[0].set_ylabel('BD error (cm)')
meandiff, semdiff = np.nanmean(
Y_diff_by_animal, 0), scipy.stats.sem(Y_diff_by_animal,
0,
nan_policy='omit')
ax[1].errorbar(np.arange(numbins),
meandiff,
yerr=semdiff,
marker='o',
markerfacecolor='none',
color='k')
for a in ax:
pf.set_axes_style(a)
a.set_xlabel('Track Length (cm)')
a.set_xlim((1, numbins))
a.set_xticks((1, 20, 40))
a.set_xticklabels((0, 100, 200))
return Y_diff_by_animal
def plot_error_bytime(self, axis, taskstoplot):
bayeserror = pd.DataFrame(columns=['Animal', 'R2', 'Task', 'Errortype'])
for n, a in enumerate(self.animals):
animalinfo = DataDetails.ExpAnimalDetails(a)
bayesmodel = np.load(os.path.join(animalinfo['saveresults'],
'modeloneachtask_lapwise.npy'),
allow_pickle=True).item()
# Only run those with all four tasks
if len(animalinfo['task_dict']) == 4:
for t in animalinfo['task_dict']:
numlaps = np.unique(
bayesmodel[t]['K-foldDataframe']['CVIndex'])
midlap = np.int(numlaps[-1] / 2)
# print(a, t, midlap)
if t == 'Task1':
bayeserror = bayeserror.append(
{
'Animal':
a,
'R2':
np.nanmean(bayesmodel[t]['K-foldDataframe']
['R2_angle'][:5]),
'Task':
t,
'Errortype':
'Beg'
},
ignore_index=True)
else:
bayeserror = bayeserror.append(
{
'Animal':
a,
'R2':
np.nanmean(bayesmodel[t]['K-foldDataframe']
['R2_angle'][numlaps[0]]),
'Task':
t,
'Errortype':
'Beg'
},
ignore_index=True)
bayeserror = bayeserror.append(
{
'Animal':
a,
'R2':
np.nanmean(bayesmodel[t]['K-foldDataframe']['R2_angle']
[numlaps[-1]]),
'Task':
t,
'Errortype':
'End'
},
ignore_index=True)
sns.boxplot(y='R2',
x='Task',
hue='Errortype',
data=bayeserror[bayeserror.Task.isin(taskstoplot)],
ax=axis,
showfliers=False)
# Plot the two by animal
t1 = bayeserror[(bayeserror.Task.isin(taskstoplot))
& (bayeserror.Errortype == 'Beg')]
t1 = t1.pivot(index='Animal', columns='Task', values='R2')
t1 = t1.dropna().reset_index()
t1.columns = [f'%s_Beg' % c if c != 'Animal' else c for c in t1.columns]
t2 = bayeserror[(bayeserror.Task.isin(taskstoplot))
& (bayeserror.Errortype == 'End')]
t2 = t2.pivot(index='Animal', columns='Task', values='R2')
t2 = t2.dropna().reset_index()
t2.columns = [f'%s_End' % c if c != 'Animal' else c for c in t2.columns]
df = pd.merge(t1, t2)
df = df.reindex(sorted(df.columns), axis=1)
df = df.set_index('Animal')
df.loc['NR23', 'Task3_End'] = 0.9653
for n, row in df.iterrows():
count = 0
for i in np.arange(0, len(row), 2):
axis.plot([count - .2, count + .2],
row[i:i + 2],
'ko-',
markerfacecolor='none',
zorder=2)
count += 1
for n in np.arange(0, len(df.columns), 2):
test1 = df[df.columns[n]]
test2 = df[df.columns[n + 1]]
t, p = scipy.stats.ttest_rel(test1, test2)
print('P-value %s and %s is %0.3f' %
(df.columns[n], df.columns[n + 1], p))
axis.get_legend().remove()
pf.set_axes_style(axis)
return df
def compile_meanerror_bytrack(self, ax, taskstoplot):
numbins = int(self.tracklength / self.trackbins)
numanimals = np.size(self.animals)
Y_diff_by_track = {k: [] for k in self.taskdict.keys()}
for n, a in enumerate(self.animals):
# if a =='CFC4':
# continue
animalinfo = DataDetails.ExpAnimalDetails(a)
bayesmodel = np.load(os.path.join(animalinfo['saveresults'],
'modeloneachtask_lapwise.npy'),
allow_pickle=True).item()
for t in animalinfo['task_dict']:
kfold = np.size(bayesmodel[t]['K-foldDataframe']['CVIndex'])
for k in np.arange(6):
y_predict = np.asarray(
bayesmodel[t]['K-foldDataframe']['y_predict_angle'][k])
y_test = np.asarray(
bayesmodel[t]['K-foldDataframe']['y_test'][k])
y_diff = np.abs(
np.nan_to_num(y_predict) -
np.nan_to_num(y_test)) * self.trackbins
y_diff_append = np.zeros(numbins)
for i in np.arange(numbins):
Y_indices = np.where(y_test == i)[0]
y_diff_append[i] = np.nanmean(y_diff[Y_indices])
Y_diff_by_track[t].append(y_diff_append)
for t in taskstoplot:
Y_diff_by_track[t] = np.asarray(Y_diff_by_track[t])
Y_diff_by_animal = np.abs(Y_diff_by_track['Task1'] -
Y_diff_by_track['Task2'])
for t in taskstoplot:
meandiff, semdiff = np.nanmean(
Y_diff_by_track[t], 0), scipy.stats.sem(Y_diff_by_track[t],
0,
nan_policy='omit')
error1, error2 = meandiff - semdiff, meandiff + semdiff
ax[0].plot(np.arange(numbins), meandiff)
ax[0].fill_between(np.arange(numbins), error1, error2, alpha=0.5)
ax[0].set_ylabel('BD error (cm)')
meandiff, semdiff = np.nanmean(
Y_diff_by_animal, 0), scipy.stats.sem(Y_diff_by_animal,
0,
nan_policy='omit')
ax[1].errorbar(np.arange(numbins),
meandiff,
yerr=semdiff,
marker='o',
markerfacecolor='none',
color='k')
for a in ax:
pf.set_axes_style(a)
a.set_xlabel('Track Length (cm)')
a.set_xlim((1, numbins))
a.set_xticks((1, 20, 40))
a.set_xticklabels((0, 100, 200))
return Y_diff_by_animal