def return_eeg(cell, radii, sigmas, eeg_coords, morph_type):
# compute current dipole moment
cdm = LFPy.CurrentDipoleMoment(cell)
P = cdm.get_transformation_matrix() @ cell.imem
# set dipole position
r_soma_syns = [cell.get_intersegment_vector(idx0=0, idx1=i)
for i in cell.synidx]
r_mid = np.average(r_soma_syns, axis=0)
if morph_type == 'l5i':
somapos = np.array([0., 0., radii[0]-1200])
else:
somapos = np.array([0., 0., radii[0] - np.max(cell.z) - 50])
dipole_pos = r_mid + somapos
cell.set_pos(x=somapos[0], y=somapos[1], z=somapos[2])
# multi_dipoles, dipole_locs = cell.get_multi_current_dipole_moments()
# t_point = 800
# P_from_multi_dipoles = np.sum(multi_dipoles[:,t_point,:],axis=0)
# print('single dipole:', P[t_point])
# print('sum of multi-dipoles', P_from_multi_dipoles)
# compute eeg with 4S model
fs_eeg = LFPy.FourSphereVolumeConductor(eeg_coords, radii=radii,
sigmas=sigmas)
eeg = fs_eeg.get_transformation_matrix(dipole_pos) @ P
eeg_multidip = fs_eeg.get_dipole_potential_from_multi_dipoles(cell)
eeg_avg = np.average(eeg, axis=0)
eeg_multidip_avg = np.average(eeg_multidip, axis=0)
# convert from mV to pV:
eeg_avg = eeg_avg * 1e9
eeg_multidip_avg = eeg_multidip_avg * 1e9
return eeg_avg, eeg_multidip_avg
def cell_w_synapse_from_sections_w_electrode(morphology, electrode_locs):
'''
Make cell and synapse objects, set spike, simulate and return cell
'''
cellParams = {
'morphology': morphology,
'cm': 1,
'Ra': 150,
'v_init': -65,
'passive': True,
'passive_parameters': {
'g_pas': 1. / 30000,
'e_pas': -65
},
'dt': 2**-6,
'tstart': -50,
'tstop': 50,
'delete_sections': False
}
electrodeParams = {
'sigma': 0.3,
'x': electrode_locs[:, 0],
'y': electrode_locs[:, 1],
'z': electrode_locs[:, 2],
}
cell = LFPy.Cell(**cellParams)
electrode = LFPy.RecExtElectrode(cell, **electrodeParams)
cdm = LFPy.CurrentDipoleMoment(cell)
synapse_parameters = {
'e': 0.,
'syntype': 'ExpSyn',
'tau': 5.,
'weight': .1,
'record_current': True,
'idx': cell.totnsegs - 1
}
synapse = LFPy.Synapse(cell, **synapse_parameters)
synapse.set_spike_times(np.array([1.]))
cell.simulate(probes=[electrode, cdm], rec_vmem=True)
return cell, electrode, cdm
def cell_w_synapse_from_sections(morphology, rec_current_dipole_moment=False):
'''
Make cell and synapse objects, set spike, simulate and return cell
'''
cellParams = {
'morphology': morphology,
'cm': 1,
'Ra': 150,
'v_init': -65,
'passive': True,
'passive_parameters': {
'g_pas': 1. / 30000,
'e_pas': -65
},
'dt': 2**-6,
'tstart': -50,
'tstop': 50,
'delete_sections': False
}
synapse_parameters = {
'e': 0.,
'syntype': 'ExpSyn',
'tau': 5.,
'weight': .001,
'record_current': True,
'idx': 1
}
cell = LFPy.Cell(**cellParams)
synapse = LFPy.Synapse(cell, **synapse_parameters)
synapse.set_spike_times(np.array([1.]))
if rec_current_dipole_moment:
probes = [LFPy.CurrentDipoleMoment(cell)]
else:
probes = []
cell.simulate(probes=probes, rec_vmem=True)
if rec_current_dipole_moment:
cell.current_dipole_moment = probes[0].data
return cell
def make_data(morphology, cell_model, rot, rz, radii, sigmas, electrode_locs,
syn_idcs, spiking, syn_input_time):
# set cell and synapse parameters
cell_parameters, synapse_parameters = set_parameters(
morphology, cell_model, spiking)
[xrot, yrot, zrot] = rot
# create four-sphere class instance
fs = LFPy.FourSphereVolumeConductor(electrode_locs,
radii=radii,
sigmas=sigmas)
# lists for storing data:
p_list = []
t_max_list = []
p_loc_list = []
lfp_single_dip_list = []
lfp_multi_dip_list = []
RE_list = []
synlocs = []
pz_traces = []
# get data from num_syns simulations
num_syns = len(syn_idcs)
for j in range(num_syns):
cell = create_cell(cell_parameters,
active=spiking,
x_rot=xrot,
y_rot=yrot,
z_rot=zrot)
## if you know synidx:
syn_idx = syn_idcs[j]
# if you only know synapse location:
# syn_loc = syn_idcs[j]
# syn_idx = cell.get_closest_idx(x=syn_loc[0], y=syn_loc[1], z=syn_loc[2])
# print('syn_idx:', syn_idx)
cell, synapse = simulate(cell, synapse_parameters, [syn_idx],
syn_input_time)
# print('cell simulated')
cell.set_pos(x=rz[0], y=rz[1], z=rz[2])
syn_loc = (cell.x[syn_idx].mean(), cell.y[syn_idx].mean(),
cell.z[syn_idx].mean())
synlocs.append((syn_loc[0], syn_loc[1], syn_loc[2]))
# compute current dipole moment and subtract DC-component
cdm = LFPy.CurrentDipoleMoment(cell)
dipoles = cdm.get_transformation_matrix() @ cell.imem
input_idx_before_input = np.argmin(
np.abs(cell.tvec - syn_input_time)) - 1
p_dc = dipoles[:, input_idx_before_input]
dipoles -= p_dc[:, None]
pz_traces.append(dipoles[2, :])
# compute timepoint with biggest dipole
timemax = [np.argmax(np.linalg.norm(np.abs(dipoles), axis=0))]
t_max_list.append(timemax)
p = dipoles[:, timemax]
# compute LFP with single dipole
# dip_loc = get_mass_center(cell, timemax)
dip_loc = get_dipole_loc(rz, syn_loc)
lfp_single_dip = fs.get_transformation_matrix(dip_loc) @ p
# print('pot from single dip computed')
# compute LFP with multi-dipole
# subtract DC-component
multi_p_319, multi_p_locs = cell.get_multi_current_dipole_moments(
[input_idx_before_input])
multi_p_dc = multi_p_319
multi_p, multi_p_locs = cell.get_multi_current_dipole_moments(timemax)
multi_p -= multi_p_dc
Ni, Nd, Nt = multi_p.shape
lfp_multi_dip = np.zeros((len(electrode_locs), Nt))
for num in range(Ni):
pot = fs.get_transformation_matrix(
multi_p_locs[num]) @ multi_p[num]
# fs.calc_potential(multi_p[num], multi_p_locs[num])
lfp_multi_dip += pot
# print('pot from multi dip computed')
# compute relative errors
RE = np.abs((lfp_single_dip - lfp_multi_dip) / lfp_multi_dip)
print('syn number: {}; syn dist from soma: {}; RE_EEG: {}'.format(
syn_idx, syn_loc[2] - rz[2], RE[-1]))
#add data to lists for storage
p_list.append(p)
p_loc_list.append(dip_loc)
lfp_single_dip_list.append(lfp_single_dip)
lfp_multi_dip_list.append(lfp_multi_dip)
RE_list.append(RE)
# Do this for only one cell for plotting
zips = []
for x, z in cell.get_idx_polygons():
zips.append(list(zip(x, z)))
zmax = np.max(cell.z)
soma_vmem = cell.vmem[0]
tvec = cell.tvec
return (p_list, pz_traces, lfp_multi_dip_list, lfp_single_dip_list,
synlocs, zips, zmax, tvec, soma_vmem)
'syntype': 'ExpSyn', # exponential synapse
'tau': 5., # synapse time constant
'weight': 0.001, # 0.001, # synapse weight
'record_current': True # record synapse current
}
# create cell with parameters in dictionary
cell = LFPy.Cell(**cell_params)
pos = syn_loc
synapse_params['idx'] = cell.get_closest_idx(x=pos[0], y=pos[1], z=pos[2])
synapse = LFPy.Synapse(cell, **synapse_params)
synapse.set_spike_times(np.array([5.]))
cell.simulate(rec_imem=True)
# compute dipole
cdm = LFPy.CurrentDipoleMoment(cell)
P = cdm.get_transformation_matrix() @ cell.imem
somapos = np.array([0., 0., 77500])
r_soma_syns = [
cell.get_intersegment_vector(idx0=0, idx1=i) for i in cell.synidx
]
r_mid = np.average(r_soma_syns, axis=0)
r_mid = somapos + r_mid / 2.
eeg_coords_top = np.array([[0., 0., radii[3] - rad_tol]])
four_sphere_top = LFPy.FourSphereVolumeConductor(eeg_coords_top, radii,
sigmas)
pot_db_4s_top = four_sphere_top.get_dipole_potential(P, r_mid)
eeg_top = np.array(pot_db_4s_top) * 1e9
##########################################################################
# Set up extracellular electrodes and devices
##########################################################################
probes = []
if PSET.COMPUTE_LFP:
electrode = LFPy.RecExtElectrode(cell=None, **PSET.electrodeParams)
probes.append(electrode)
if PSET.COMPUTE_ECOG:
ecog_electrode = LFPy.RecMEAElectrode(cell=None, **PSET.ecogParameters)
probes.append(ecog_electrode)
if PSET.COMPUTE_P:
current_dipole_moment = LFPy.CurrentDipoleMoment(cell=None)
probes.append(current_dipole_moment)
##########################################################################
# Recording of additional variables
##########################################################################
if RANK == 0:
network.t = neuron.h.Vector()
network.t.record(neuron.h._ref_t)
else:
network.t = None
##########################################################################
# run simulation, gather results across all RANKs
##########################################################################
# Assert that connect routines has finished across RANKS before starting
def test_Network_01(self):
cellParameters = dict(
morphology=os.path.join(LFPy.__path__[0], 'test',
'ball_and_sticks_w_lists.hoc'),
templatefile=os.path.join(LFPy.__path__[0], 'test',
'ball_and_stick_template.hoc'),
templatename='ball_and_stick_template',
templateargs=None,
passive=False,
dt=2**-3,
tstop=100,
delete_sections=False,
)
populationParameters = dict(
CWD=None,
CELLPATH=None,
Cell=LFPy.NetworkCell,
cell_args=cellParameters,
pop_args=dict(radius=100, loc=0., scale=20.),
rotation_args=dict(x=0, y=0),
POP_SIZE=4,
name='test',
)
networkParameters = dict(dt=2**-3,
tstart=0.,
tstop=100.,
v_init=-65.,
celsius=6.3,
OUTPUTPATH='tmp_testNetworkPopulation')
# set up
network = LFPy.Network(**networkParameters)
network.create_population(**populationParameters)
connectivity = network.get_connectivity_rand(pre='test',
post='test',
connprob=0.5)
# connect
network.connect(pre='test', post='test', connectivity=connectivity)
# create CurrentDipoleMoment object for probing
probes = [LFPy.CurrentDipoleMoment(cell=None)]
# run simutation, record using probe, resolve into populations
SPIKES, LFP = network.simulate(probes=probes,
rec_pop_contributions=True)
# current dipole momemnt
P = probes[0].data
# test output
for population in network.populations.values():
for cell in population.cells:
self.assertTrue(np.all(cell.somav == network.v_init))
self.assertTrue(np.all(P['test'] == 0.))
self.assertTrue(P.shape[1] == cell.somav.size)
network.pc.gid_clear()
os.system('rm -r tmp_testNetworkPopulation')
for population in network.populations.values():
for cell in population.cells:
cell.strip_hoc_objects()
neuron.h('forall delete_section()')
def run_cell_simulation(make_ca_spike, dt, cell_name, grid_elec_params,
elec_params):
T = 100
if cell_name == 'almog':
model_folder = join('cell_models', 'almog')
neuron.load_mechanisms(model_folder)
os.chdir(model_folder)
cell_parameters = {
'morphology': join('A140612.hoc'),
'v_init': -62,
'passive': False,
'nsegs_method': None,
'dt': dt, # [ms] Should be a power of 2
'tstart': -200, # [ms] Simulation start time
'tstop': T, # [ms] Simulation end time
'custom_code':
[join('cell_model.hoc')] # Loads model specific code
}
cell = LFPy.Cell(**cell_parameters)
os.chdir(join('..', '..'))
cell.set_rotation(x=np.pi / 2, y=0.1)
elif cell_name == 'hay':
model_folder = join('cell_models', 'hay', 'L5bPCmodelsEH')
neuron.load_mechanisms(join(model_folder, "mod"))
##define cell parameters used as input to cell-class
cellParameters = {
'morphology':
join(model_folder, "morphologies", "cell1.asc"),
'templatefile': [
join(model_folder, "models", "L5PCbiophys3.hoc"),
join(model_folder, "models", "L5PCtemplate.hoc")
],
'templatename':
'L5PCtemplate',
'templateargs':
join(model_folder, "morphologies", "cell1.asc"),
'passive':
False,
'nsegs_method':
None,
'dt':
dt,
'tstart':
-200,
'tstop':
T,
'v_init':
-60,
'celsius':
34,
'pt3d':
True,
}
cell = LFPy.TemplateCell(**cellParameters)
cell.set_rotation(x=np.pi / 2, y=0.1)
plot_idxs = [cell.somaidx[0], cell.get_closest_idx(z=500)]
idx_clr = {idx: ['b', 'orange'][num] for num, idx in enumerate(plot_idxs)}
if cell_name == 'hay':
weights = [0.07, 0.15]
elif cell_name == 'almog':
weights = [0.05, 0.15]
delay = 30
synapse_s = LFPy.Synapse(cell,
idx=cell.get_closest_idx(z=0),
syntype='Exp2Syn',
weight=weights[0],
tau1=0.1,
tau2=1.)
synapse_s.set_spike_times(np.array([delay]))
synapse_a = None
if make_ca_spike:
synapse_a = LFPy.Synapse(cell,
idx=cell.get_closest_idx(z=400),
syntype='Exp2Syn',
weight=weights[1],
tau1=0.1,
tau2=10.)
synapse_a.set_spike_times(np.array([delay]))
cell.simulate(rec_imem=True, rec_vmem=True)
print("MAX |I_mem(soma, apic)|: ",
np.max(np.abs(cell.imem[plot_idxs]), axis=1))
elec = LFPy.RecExtElectrode(cell, **elec_params)
elec_LFP = 1e3 * elec.get_transformation_matrix() @ cell.imem
elec_LFP -= elec_LFP[:, 0, None]
grid_electrode = LFPy.RecExtElectrode(cell, **grid_elec_params)
grid_LFP = 1e3 * grid_electrode.get_transformation_matrix() @ cell.imem
grid_LFP -= grid_LFP[:, 0, None]
cdm = LFPy.CurrentDipoleMoment(cell)
cdm = cdm.get_transformation_matrix() @ cell.imem
cdm -= cdm[:, 0, None]
cell_data_dict = {
"vmem": cell.vmem.copy(),
"tvec": cell.tvec.copy(),
"elec_LFP": elec_LFP.copy(),
"grid_LFP": grid_LFP.copy(),
"cdm": cdm.copy(),
"cell_x": cell.x.copy(),
"cell_z": cell.z.copy(),
}
synapse_s = None
synapse_a = None
cell.__del__()
return cell_data_dict, idx_clr, plot_idxs
def make_data(morphology,
syninds,
dip_loc=None,
cell_model=None,
x_rot=0,
y_rot=0,
z_rot=0,
active=False):
sigma = 0.3 #extracellular conductivity
k = 1e6 # convert from mV to nV
# compute LFP close to neuron
# set up electrodes close to neuron
X, Z = np.meshgrid(np.linspace(-550, 550, 101),
np.linspace(-250, 850, 101))
Y = np.zeros(X.shape)
# simulate cell
(cell_parameters, synapse_parameters,
grid_electrode_parameters) = set_parameters(morphology,
X,
Y,
Z,
sigma=sigma,
cell_model=cell_model)
cell, synapse, grid_electrode_LFP = simulate(cell_parameters,
synapse_parameters,
grid_electrode_parameters,
syninds,
x_rot=x_rot,
y_rot=y_rot,
z_rot=z_rot,
active=active)
# multicompartment modeling of LFP close to cell
cb_LFP_close = grid_electrode_LFP * k
# multi-dipole modeling of LFP close to cell
multi_dips, multi_dip_locs = cell.get_multi_current_dipole_moments()
inf_vol = LFPy.InfiniteVolumeConductor(sigma)
gridpoints_close = np.array([X.flatten(), Y.flatten(), Z.flatten()]).T
multi_dip_LFP_close = inf_vol.get_multi_dipole_potential(
cell, gridpoints_close) * k
# single-dipole modeling of LFP close to cell
cdm = LFPy.CurrentDipoleMoment(cell)
single_dip = cdm.get_transformation_matrix() @ cell.imem
syninds = cell.synidx
if dip_loc is not None:
r_mid = dip_loc
else:
r_soma_syns = [
cell.get_intersegment_vector(idx0=0, idx1=i) for i in syninds
]
r_mid = np.average(r_soma_syns, axis=0)
r_mid = r_mid / 2. + cell.somapos
db_LFP_close = inf_vol.get_dipole_potential(single_dip,
gridpoints_close - r_mid) * k
# compute LFP far from neuron
# set up electrodes
X_f, Z_f = np.meshgrid(np.linspace(-15000, 15001, 101),
np.linspace(-15000, 15000, 101))
Y_f = np.zeros(X.shape)
grid_electrode_parameters = {
'x': X_f.flatten(),
'y': Y_f.flatten(),
'z': Z_f.flatten()
}
# simulate cell
cell, synapse, grid_electrode_far_LFP = simulate(cell_parameters,
synapse_parameters,
grid_electrode_parameters,
syninds,
x_rot=x_rot,
y_rot=y_rot,
z_rot=z_rot,
active=active)
# multicompartment modeling of LFP far from cell
cb_LFP_far = grid_electrode_far_LFP * k
# multi-dipole modeling of LFP far from cell
gridpoints_far = np.array([X_f.flatten(), Y_f.flatten(), Z_f.flatten()]).T
multi_dip_LFP_far = inf_vol.get_multi_dipole_potential(
cell, gridpoints_far) * k
# single-dipole modeling of LFP far from cell
db_LFP_far = inf_vol.get_dipole_potential(single_dip,
gridpoints_far - r_mid) * k
cdm = LFPy.CurrentDipoleMoment(cell)
single_dip = cdm.get_transformation_matrix() @ cell.imem
time_max = np.argmax(np.abs(np.linalg.norm(single_dip, axis=0)))
LFP_max = 100. #nV #np.round(np.max(np.abs(grid_electrode_LFP[:, time_max])))
return (cell, cb_LFP_close, cb_LFP_far, multi_dip_LFP_close,
multi_dip_LFP_far, db_LFP_close, db_LFP_far, LFP_max, time_max,
multi_dips, multi_dip_locs, single_dip, r_mid, X, Z, X_f, Z_f)
