def dict_to_bioalignment(d, alphabet='generic_alphabet', sorted=True):
"""
Create a BioPython MultipleSequenceAlignment
from a dict with pairs consisting of id and sequence.
"""
alignment = MultipleSeqAlignment([])
bio_alphabet = getattr(Bio.Alphabet, alphabet)
for id, seq in d.items():
seq_record = SeqRecord(Seq(seq, alphabet=bio_alphabet), id=id)
alignment.append(seq_record)
if sorted:
alignment.sort()
return alignment
def retrieve_alignment(tre, alnpath, taxonset=range(0, 101), delimiter='_'):
"""
Parameters
----------------
tre : single-copy treeswift tree generated from James's code
alnpath : path to the phylip formatted alignment of the genes. The row labels should be a superset of the leafset of 'tre'
seqlen : sequence length parameter, only the first seqlen columns are taken from the MSA
taxonset: set, the taxon set of the entire dataset
Returns the MSA that corresponds to the input tree.
"""
aln = AlignIO.read(open(alnpath), "phylip")
seqlen = len(aln[0].seq)
blank = "-" * seqlen
whitelist = set(tre.labels(True, False))
rest = set(taxonset)
#print(rest)
res = MultipleSeqAlignment([])
for r in aln[:, :seqlen]:
if r.id in whitelist:
rid = r.id.split(delimiter)[0]
rid_i = rid
res.append(SeqRecord(r.seq, id=rid))
rest.remove(rid_i)
for rst in rest:
res.append(SeqRecord(Seq(blank), id=str(rst)))
res.sort()
return res
def test_trimal2(alb_resources, hf):
tester = Alb.trimal(alb_resources.get_one("o p n"), 'all')
assert hf.buddy2hash(tester) == "8faaf09741ddb3137653cb77ee66974a"
tester = alb_resources.get_one("o p n")
tester.alignments[0]._records = tester.alignments[0]._records[:5]
Alb.trimal(tester, 'clean')
assert hf.buddy2hash(tester) == "93a2aa21e6baf5ca70eb2de52ae8dbea"
tester = alb_resources.get_one("o p n")
tester_dir = TEMPDIR.subdir()
tester.write("%s%strimal" % (tester_dir, os.path.sep))
assert hf.buddy2hash(Alb.trimal(
tester, 'gappyout')) == "2877ecfb201fc35211a4625f34c7afdd"
""" Probably not a good idea to be calling binaries like this...
real_trimal = Popen("trimal -in %s%strimal -gappyout" % (tester_dir, os.path.sep),
stdout=PIPE, shell=True).communicate()
real_trimal = real_trimal[0].decode()
with open("%s%strimal" % (tester_dir, os.path.sep), "w") as ofile:
ofile.write(real_trimal)
tester = Alb.AlignBuddy("%s%strimal" % (tester_dir, os.path.sep))
assert hf.buddy2hash(tester) == "2877ecfb201fc35211a4625f34c7afdd"
"""
records = [
SeqRecord(Seq("A--G-")),
SeqRecord(Seq("--T--")),
SeqRecord(Seq("--TG-")),
SeqRecord(Seq("A---C"))
]
tester = Alb.AlignBuddy([MultipleSeqAlignment(records)])
Alb.trimal(tester, "gappyout")
assert "".join([str(rec.seq) for rec in tester.records()]) == ""
def test_get_subalignment_sequence_order_maintained(self):
"""
Sequences given rearranged are still output in input order
"""
result = AlignedSeq.get_sub_alignment_by_list_id(["s3", "s1"], self.alignment)
expected = MultipleSeqAlignment([self.alignment[0], self.alignment[2]])
self.assertTrue(msas_equal(expected, result))
def identify_polymorphisms(self):
self.alignment = MultipleSeqAlignment(self.sequences)
for col in range(self.alignment.get_alignment_length()):
polmorphs = set(self.alignment[:,col])
if len(polmorphs) > 1:
self.polymorphisms[col] = polmorphs
def test_get_subalignment_with_interval(self):
result = AlignedSeq.get_sub_alignment_by_list_id(
["s2", "s3"], self.alignment, [0, 2]
)
expected = MultipleSeqAlignment(
[SeqRecord(Seq("C--"), id="s2"), SeqRecord(Seq("AAT"), id="s3"),]
)
self.assertTrue(msas_equal(expected, result))
def get_sub_alignment_by_list_id(self,
id_list: List[str],
alignment: MultipleSeqAlignment,
interval=None):
list_records = [record for record in alignment if record.id in id_list]
sub_alignment = MultipleSeqAlignment(list_records)
if interval:
sub_alignment = sub_alignment[:, interval[0]:interval[1] + 1]
return sub_alignment
def setUpClass(cls):
cls.alignment = MultipleSeqAlignment(
[
SeqRecord(Seq("AAAT"), id="s1"),
SeqRecord(Seq("C--C"), id="s2"),
SeqRecord(Seq("AATT"), id="s3"),
SeqRecord(Seq("GNGG"), id="s4"),
]
)
def test_two_identical_sequences_clustered_together(self):
alignment = MultipleSeqAlignment(
[
SeqRecord(Seq("AAAT"), id="s1"),
SeqRecord(Seq("AAAT"), id="s2"),
SeqRecord(Seq("C-CC"), id="s3"),
]
)
result = AlignedSeq.kmeans_cluster_seqs_in_interval([0, 3], alignment, 1)
self.assertEqual([["s1", "s2"], ["s3"]], result)
def test_sequences_in_short_interval_separate_clusters(self):
alignment = MultipleSeqAlignment(
[
SeqRecord(Seq("AAAT"), id="s1"),
SeqRecord(Seq("AATT"), id="s2"),
SeqRecord(Seq("AAGT"), id="s3"),
]
)
result = AlignedSeq.kmeans_cluster_seqs_in_interval([0, 3], alignment, 5)
# Each sequence is below min_match_len (5), so goes into own cluster
self.assertEqual([["s1"], ["s2"], ["s3"]], result)
def test_all_sequences_below_min_match_len(self):
alignment = MultipleSeqAlignment(
[
SeqRecord(Seq("AA---AT"), id="s1"),
SeqRecord(Seq("AA---TT"), id="s2"),
SeqRecord(Seq("CA--CAT"), id="s3"),
]
)
result = AlignedSeq.kmeans_cluster_seqs_in_interval(
[0, len(alignment[0])], alignment, 6
)
self.assertEqual([["s1"], ["s2"], ["s3"]], result)
def modeller_automodel(self, query: SeqRecord, results: Path,
num_align: int, atom_files_dir: Path):
from modeller import environ
from modeller.automodel import automodel
for model_index, r in enumerate(
np.load(results, allow_pickle=True)[:num_align]):
try:
aln = AlignIO.read(StringIO(r[-2][0]), 'clustal')
except:
logging.error(
f'Failed to parse alignment: {r[0]} -> {r[2]} -> {r[4]} -> {r[6]}'
)
continue
assert query.id == aln[0].id and aln[-1].id == r[-3]
q_rec, t_rec = self._remove_gaps(aln[0], aln[-1])
try:
t_rec = self._remove_missing_res(
t_rec, (atom_files_dir / aln[-1].id[2:4] /
f'{aln[-1].id}.ent').resolve().as_posix())
except FileNotFoundError as e:
logging.exception(e)
continue
q_rec.name, t_rec.name = '', ''
q_rec.description = f'sequence:{q_rec.id}::::::::'
t_rec.description = f'structureX:{t_rec.id}::{t_rec.id[-2].upper()}::{t_rec.id[-2].upper()}::::'
aln = MultipleSeqAlignment([q_rec, t_rec])
out_d = results.resolve().parent
if (out_d / f'{aln[0].id}_{model_index+1}.pdb').exists():
continue
cwd = os.getcwd()
with tempfile.TemporaryDirectory() as tmpdir:
try:
os.chdir(tmpdir)
AlignIO.write(aln, 'aln.pir', 'pir')
env = environ()
env.io.atom_files_directory = [
(atom_files_dir / aln[1].id[2:4]).resolve().as_posix()
]
mod = automodel(env,
'aln.pir',
knowns=[aln[1].id],
sequence=aln[0].id)
mod.make()
shutil.copy(
list(Path().glob('*.B*.pdb'))[0],
out_d / f'{aln[0].id}_{model_index+1}.pdb')
except Exception as e:
logging.error(
f'knowns=[{aln[1].id}], sequence={aln[0].id}')
logging.exception(e)
finally:
os.chdir(cwd)
def _parse(self):
for i, l in enumerate(self.stdout):
if re.match('^\(":" denotes', l):
a = SeqRecord(Seq(self.stdout[i + 1],
alphabet=generic_protein),
id='Protein_A')
b = SeqRecord(Seq(self.stdout[i + 3],
alphabet=generic_protein),
id='Protein_B')
self.alignment = MultipleSeqAlignment([a, b])
break
for l in self.stdout:
if re.match('^TM-score', l):
self.tmscore = float(
l.split('=')[1].split('(')[0].replace(' ', ''))
break
for l in self.stdout:
if re.match('^MaxSub-score', l):
self.maxsub = float(
l.split('=')[1].split('(')[0].replace(' ', ''))
break
for l in self.stdout:
if re.match('^GDT-TS-score', l):
self.gdtts = float(
l.split('=')[1].split('%')[0].replace(' ', ''))
break
for l in self.stdout:
if re.match('^GDT-HA-score', l):
self.gdtha = float(
l.split('=')[1].split('%')[0].replace(' ', ''))
break
for l in self.stdout:
if re.match('^RMSD of', l):
self.rmsd = float(l.split('=')[1].replace(' ', ''))
break
for l in self.stdout:
if re.match('^Number of residues in common', l):
self.num_res_in_common = int(l.split('=')[1].replace(' ', ''))
break
for l in self.stdout:
if re.match('^Structure1: ', l):
self.len_a = int(
l.split('=')[1].split('(')[0].replace(' ', ''))
break
for l in self.stdout:
if re.match('^Structure2: ', l):
self.len_b = int(
l.split('=')[1].split('(')[0].replace(' ', ''))
break
def test_one_long_one_short_sequence_separate_and_ordered_clusters(self):
alignment = MultipleSeqAlignment(
[
SeqRecord(Seq("AATTAATTATATAATAAC"), id="s1"),
SeqRecord(Seq("A--------------AAT"), id="s2"),
]
)
order_1 = AlignedSeq.kmeans_cluster_seqs_in_interval(
[0, len(alignment[0])], alignment, 5
)
self.assertEqual(order_1, [["s1"], ["s2"]])
order_2 = AlignedSeq.kmeans_cluster_seqs_in_interval(
[0, len(alignment[0])], alignment[::-1], 5
)
self.assertEqual(order_2, [["s2"], ["s1"]])
def _parse(self):
for i, l in enumerate(self.stdout):
if re.match('^TM-score=', l):
self.tmscore = (float(self.stdout[i].split(' ')[1]),
float(self.stdout[i + 1].split(' ')[1]))
break
for i, l in enumerate(self.stdout):
if re.match('^\(":" denotes', l):
a = SeqRecord(Seq(self.stdout[i + 1],
alphabet=generic_protein),
id=Path(self.protein_A).stem + '&' +
Path(self.protein_B).stem,
description=f'TM-score={self.tmscore[0]}')
b = SeqRecord(Seq(self.stdout[i + 3],
alphabet=generic_protein),
id=Path(self.protein_B).stem + '&' +
Path(self.protein_A).stem,
description=f'TM-score={self.tmscore[1]}')
self.alignment = MultipleSeqAlignment([a, b])
break
def test_first_sequence_placed_in_first_cluster(self):
"""
Runs kmeans clustering on randomly generated multiple sequence alignments
"""
seq_len = 20
num_seqs = 20
bases = list(standard_bases)
# Function has different behaviour at below and above seq_len
for seq_len in [seq_len - 1, seq_len + 1]:
with self.subTest(min_match_len=seq_len):
for _ in range(20): # Run on a number of random alignments
records = []
for i in range(num_seqs):
rand_seq = "".join(
[random.choice(bases) for _ in range(seq_len)]
)
records.append(SeqRecord(Seq(rand_seq), id=f"s{i}"))
alignment = MultipleSeqAlignment(records)
result = AlignedSeq.kmeans_cluster_seqs_in_interval(
[0, seq_len - 1], alignment, 1
)
self.assertTrue(result[0][0] == "s0")
def _local_align(self, record_a: SeqRecord, record_b: SeqRecord,
open_gap_score: int):
aligner = Align.PairwiseAligner()
aligner.mode = 'local'
aligner.substitution_matrix = substitution_matrices.load('BLOSUM62')
aligner.open_gap_score = open_gap_score
aligner.extend_gap_score = -1
aln = aligner.align(
record_a.seq.ungap('-').upper(),
record_b.seq.ungap('-').upper())[0]
seq_a = Seq(
str(aln).splitlines()[0].replace(' ', '-'), generic_protein)
seq_b = Seq(
str(aln).splitlines()[2].replace(' ', '-'), generic_protein)
return MultipleSeqAlignment([
SeqRecord(seq_a, id=record_a.id),
SeqRecord(seq_b, id=record_b.id)
],
annotations={
'score': aln.score,
'path': aln.path,
'aligned': aln.aligned
})
counter -
1] + " but gene " + keyList[n] + " (has " + j + ")\n"
n = n + 1
counter = counter + 1
print "I can correct these spelling mistakes...If you want me to..;) \n Do you?? \n 1. Yes \n 2. No \n"
choice = input('\n')
while choice == 1:
print "Enter the correct taxon name \n"
usrInp = raw_input('\n')
for filename in fileList:
handle = open(filename, 'rU')
record = list(SeqIO.parse(handle, 'nexus'))
msa = MultipleSeqAlignment(record)
for i, val in enumerate(msa):
if 1.0 > float([x == y for (x, y) in zip(usrInp, msa[i].id)
].count(True)) / len(msa[i].id) > 0.8:
msa[i].id = usrInp
else:
print "No Spelling mistakes found in file %s \n" % filename
fp = open(filename, 'w')
SeqIO.write(msa, fp, typeList[file_format - 1])
handle.close()
fp.close()
print "Want me to do some more editing?? \n Well! I can do this whole day \n What about you?? \n 1. Yes \n 2. No \n"
choice = input('\n')
#print(dir(multiple_alignment))
#sys.exit(0)
seqs = []
for seqrec in multiple_alignment:
#print(dir(seqrec))
try:
name_id = seqrec.id.partition(" ")[0].partition(".")[0]
#print(name_id)
seqrec.id = organisms[name_id]
if seqrec.id in organisms.values():
seqs.append(seqrec)
except:
continue
new_alignment = MultipleSeqAlignment(records=seqs)
#print(len(new_alignment))
AlignIO.write(new_alignment, "mm9_" + rg.name + ".fa", "fasta")
process = subprocess.Popen([
"/home/joseph/Apps/PhyloCSF/PhyloCSF", "29mammals",
"mm9_" + rg.name + ".fa", "--removeRefGaps", "--strategy=omega",
"--orf=StopStop3", "--minCodons=25", "--frames=3"
],
stdout=subprocess.PIPE)
out, err = process.communicate()
print(out)
#print(out.split("\t")[2])
#print(out.split("\t")[3])
#print(out.split("\t")[4])
seqAA = safe_translate(seqCDS_ungapped)
scoreAA, refalnAA, seqalnAA = align_pairwise(refAA, seqAA)
if scoreAA < 0 or sum(
seqAA.count(x)
for x in ['*', 'X']) > 5 or refalnAA.count('-') > 5:
print(seq.id, "didn't translate properly")
continue
seqCDS_aln = seq5pUTR
pos = 0
for aa_ref, aa_seq in zip(refalnAA, seqalnAA):
if aa_seq == '-':
seqCDS_aln += '---'
# if the nucleotide sequence is gapped
# (i.e. because of missing data at the 5p and 3p end, advance pos)
if seqCDS_ungapped[pos:pos + 3] == '---':
pos += 3
else:
if len(seqCDS_ungapped) >= pos + 3:
seqCDS_aln += seqCDS_ungapped[pos:pos + 3]
else:
seqCDS_aln += '---'
pos += 3
seq.seq = Seq.Seq(''.join(seqCDS_aln) + seq3pUTR)
alignment.append(seq)
# output
AlignIO.write(MultipleSeqAlignment(alignment), args.output, 'fasta')
def test_ambiguous_sequences_in_short_interval_separate_clusters(self):
alignment = MultipleSeqAlignment(
[SeqRecord(Seq("ARAT"), id="s1"), SeqRecord(Seq("WAAT"), id="s2"),]
)
result = AlignedSeq.kmeans_cluster_seqs_in_interval([0, 3], alignment, 5)
self.assertEqual([["s1"], ["s2"]], result)
def test_get_subalignment_sequence_order_maintained2(self):
result = AlignedSeq.get_sub_alignment_by_list_id(["s1", "s3"], self.alignment)
expected = MultipleSeqAlignment([self.alignment[0], self.alignment[2]])
self.assertTrue(msas_equal(expected, result))
def test_one_seq_returns_single_id(self):
alignment = MultipleSeqAlignment([SeqRecord(Seq("AAAT"), id="s1")])
result = AlignedSeq.kmeans_cluster_seqs_in_interval([0, 3], alignment, 1)
self.assertEqual(result, [["s1"]])
def impute_ancestors_dnapars(seqs,
gl_seq,
scratch_dir,
gl_name='germline',
verbose=True):
"""
Compute ancestral states via maximum parsimony
@param seqs: list of sequences
@param gl_seq: germline sequence
@param scratch_dir: where to write intermediate dnapars files
@param gl_name: name of germline (must be less than 10 characters long)
@return genes_line: information needed to output imputed germline data
@return seqs_line: information needed to output imputed sequence data
"""
from gctree.bin.phylip_parse import parse_outfile
assert (len(gl_name) < 10)
infile, config, outfile = [
os.path.join(scratch_dir, fname) for fname in [
'infile',
'dnapars.cfg',
'outfile',
]
]
aln = MultipleSeqAlignment([SeqRecord(Seq(gl_seq), id=gl_name)])
# sequence ID must be less than ten characters, but also dnapars sets internal node
# names to 1, 2, 3, ..., so name them numbers descending from 100 million, hoping
# we won't ever have a clone that big...
for idx, seq in enumerate(seqs):
aln.append(SeqRecord(Seq(seq), id=str(99999999 - idx)))
# dnapars uses the name "infile" as default input phylip file
with open(infile, 'w') as phylip_file:
phylip_file.write(aln.format('phylip'))
# and we need to tell it the line where the root sequence occurs
with open(infile, 'r') as phylip_file:
for lineno, line in enumerate(phylip_file):
if line.startswith(gl_name):
naive_idx = str(lineno)
# arcane user options for dnapars
# 'O', naive_idx: the location of the outgroup root
# 'S', 'Y': less thorough search; runs much faster but output is less exhaustive
# 'J', 13, 10: randomize input ("jumble") using seed 13 and jumbling 10 times
# 4: print out steps in each site (to get all nucleotide info)
# 5: print sequences in at all nodes (to get ancestors)
# '.': use dot-differencing for display
# 'Y': accept these options
with open(config, 'w') as cfg_file:
cfg_file.write('\n'.join(
['O', naive_idx, 'S', 'Y', 'J', '13', '10', '4', '5', '.', 'Y']))
# defer to command line to construct parsimony trees and ancestral states
# dnapars has weird behavior if outfile and outtree already exist o_O
cmd = [
'cd', scratch_dir, '&& rm -f outfile outtree && dnapars <',
os.path.basename(config), '> dnapars.log'
]
if verbose:
print "Calling:", " ".join(cmd)
res = subprocess.call([" ".join(cmd)], shell=True)
# phew, finally got some trees
trees = parse_outfile(outfile, countfile=None, naive=gl_name)
# take first parsimony tree
genes_line = []
seq_line = []
for idx, descendant in enumerate(trees[0].traverse('preorder')):
if descendant.is_root():
descendant.name = gl_name
else:
# use dummy name for internal node sequences
descendant.name = '-'.join([descendant.up.name, descendant.name])
if [descendant.up.name,
descendant.up.sequence.lower()] not in genes_line:
genes_line.append(
[descendant.up.name,
descendant.up.sequence.lower()])
seq_line.append([
descendant.up.name, descendant.name,
descendant.sequence.lower()
])
return genes_line, seq_line
def test_two_seqs_one_below_min_match_len_separate_clusters(self):
alignment = MultipleSeqAlignment(
[SeqRecord(Seq("AATTTAT"), id="s1"), SeqRecord(Seq("AA---AT"), id="s2")]
)
result = AlignedSeq.kmeans_cluster_seqs_in_interval([0, 5], alignment, 5)
self.assertEqual(result, [["s1"], ["s2"]])
def get_sub_alignment_by_list_id(self, list_of_id, interval=None):
list_records = [record for record in self.alignment if record.id in list_of_id]
sub_alignment = MultipleSeqAlignment(list_records)
if interval:
sub_alignment = sub_alignment[:, interval[0] : interval[1] + 1]
return sub_alignment
class PolymorphismSampler(object):
"""docstring for PolymorphismSampler"""
def __init__(self):
super(PolymorphismSampler, self).__init__()
self.sequences = None
self.alignment = None
self.polymorphisms = dict()
self.subsampled = set()
self.num_positions = []
self.num_polymorphisms = []
def read_sequences(self, handle):
sequences = [s for s in SeqIO.parse(handle, 'fasta')]
counts = Counter([len(s) for s in sequences])
mc_length = counts.most_common(1)[0][0]
filtered_sequences = list()
for s in sequences:
if len(s.seq) == mc_length:
filtered_sequences.append(s)
self.sequences = filtered_sequences
def identify_polymorphisms(self):
self.alignment = MultipleSeqAlignment(self.sequences)
for col in range(self.alignment.get_alignment_length()):
polmorphs = set(self.alignment[:,col])
if len(polmorphs) > 1:
self.polymorphisms[col] = polmorphs
def number_of_polymorphisms(self):
total_polymorphisms = 0
for pos, polymorphs in self.polymorphisms.items():
total_polymorphisms += len(polymorphs)
return total_polymorphisms
def subsample(self):
while len(self.polymorphisms.keys()) > 0:
try:
# Choose a seqrecord at random, based on LH sampling criteria.
pos = choice(list(self.polymorphisms.keys()))
letter = choice(list(self.polymorphisms[pos]))
filtered = MultipleSeqAlignment([s for s in self.alignment if s[pos] == letter])
seqrecord = choice(filtered)
self.subsampled.add(seqrecord)
# Remove polymorphisms
for pos in self.polymorphisms.keys():
if seqrecord.seq[pos] in self.polymorphisms[pos]:
self.polymorphisms[pos].remove(seqrecord.seq[pos])
# Update data
self.polymorphisms = {k:v for k,v in self.polymorphisms.items() if len(v) > 0}
self.num_polymorphisms.append(self.number_of_polymorphisms())
self.num_positions.append(len(self.polymorphisms.keys()))
except IndexError:
break
def _aligned_number(self, aln: MultipleSeqAlignment):
return len([
i for i in range(aln.get_alignment_length())
if '-' not in aln[:, i]
])
def search_layer2_evalue_sum_i_blast_merged(self, query: SeqRecord,
graphml: Path, num_align: int):
graph = networkx.read_graphml(graphml)
path = []
for nei1 in graph.neighbors(query.id):
if 'UniRef50' not in graph.nodes[nei1]['labels'].split(':'):
continue
for nei2 in graph.neighbors(nei1):
if 'UniRef50' not in graph.nodes[nei2]['labels'].split(':'):
continue
for nei3 in graph.neighbors(nei2):
if 'SCOP95' not in graph.nodes[nei3]['labels'].split(':'):
continue
score = graph.get_edge_data(query.id, nei1)['evalue'] \
+ graph.get_edge_data(nei1, nei2)['evalue'] + graph.get_edge_data(nei2, nei3)['evalue']
path.append([
query.id, None, nei1, None, nei2, None, nei3, None,
score
])
path = sorted(path, key=lambda _: _[-1])
# dedup
results, seen = [], []
for p in path:
if p[-3] in seen:
continue
seen.append(p[-3])
results.append(p)
for r in results[:num_align]:
n1_seq = query if r[2] == query.id else self._get_seq(
'uniref50', r[2])
n2_seq = query if r[4] == query.id else self._get_seq(
'uniref50', r[4])
n3_seq = query if r[6] == query.id else self._get_seq(
'scop95', r[6])
with tempfile.TemporaryDirectory() as t:
tmpdir = Path(t)
SeqIO.write(query, tmpdir / 'query.fasta', 'fasta')
SeqIO.write(n1_seq, tmpdir / 'n1.fasta', 'fasta')
r[1], _ = NcbideltablastCommandline(
query=(tmpdir / 'query.fasta').as_posix(),
subject=(tmpdir / 'n1.fasta').as_posix(),
use_sw_tback=True,
outfmt=5,
rpsdb='cdd_delta')()
hsp1 = SearchIO.read(StringIO(r[1]), 'blast-xml')[0][0]
rec1, rec2 = hsp1.aln[0], hsp1.aln[1]
seq1, seq2 = rec1.seq.tomutable(), rec2.seq.tomutable()
aln1 = hsp1.aln
SeqIO.write(aln1[1], tmpdir / 'n1.fasta', 'fasta')
SeqIO.write(n2_seq, tmpdir / 'n2.fasta', 'fasta')
r[3], _ = NcbideltablastCommandline(
query=(tmpdir / 'n1.fasta').as_posix(),
subject=(tmpdir / 'n2.fasta').as_posix(),
use_sw_tback=True,
outfmt=5,
rpsdb='cdd_delta')()
hsp2 = SearchIO.read(StringIO(r[3]), 'blast-xml')[0][0]
rec3, rec4 = hsp2.aln[0], hsp2.aln[1]
seq3, seq4 = rec3.seq.tomutable(), rec4.seq.tomutable()
aln2 = hsp2.aln
SeqIO.write(aln2[1], tmpdir / 'n2.fasta', 'fasta')
SeqIO.write(n3_seq, tmpdir / 'n3.fasta', 'fasta')
r[5], _ = NcbideltablastCommandline(
query=(tmpdir / 'n2.fasta').as_posix(),
subject=(tmpdir / 'n3.fasta').as_posix(),
use_sw_tback=True,
outfmt=5,
rpsdb='cdd_delta')()
try:
hsp3 = SearchIO.read(StringIO(r[5]), 'blast-xml')[0][0]
except IndexError:
print(hsp1)
r[7] = (None, None)
continue
rec5, rec6 = hsp3.aln[0], hsp3.aln[1]
seq5, seq6 = rec5.seq.tomutable(), rec6.seq.tomutable()
seq3 = '-' * hsp2.query_start + seq3
seq4 = '-' * hsp2.query_start + seq4
seq5 = '-' * (hsp2.query_start + hsp3.query_start) + seq5
seq6 = '-' * (hsp2.query_start + hsp3.query_start) + seq6
for i in range(hsp2.query_start, len(seq2)):
if i >= len(seq3):
seq3.append('-')
seq4.append('-')
elif seq2[i] == '-' and seq3[i] != '-':
seq3.insert(i, '-')
seq4.insert(i, '-')
elif seq3[i] == '-' and seq2[i] != '-':
seq2.insert(i, '-')
seq1.insert(i, '-')
append = len(seq2) - len(seq3)
seq3 = seq3 + '-' * append
seq4 = seq4 + '-' * append
for i in range(hsp2.query_start + hsp3.query_start, len(seq4)):
if i >= len(seq5):
seq5.append('-')
seq6.append('-')
elif seq4[i] == '-' and seq5[i] != '-':
seq5.insert(i, '-')
seq6.insert(i, '-')
elif seq5[i] == '-' and seq4[i] != '-':
seq4.insert(i, '-')
seq3.insert(i, '-')
seq2.insert(i, '-')
seq1.insert(i, '-')
append = len(seq4) - len(seq5)
seq5 = seq5 + '-' * append
seq6 = seq6 + '-' * append
rec1.seq = seq1
rec2.seq = seq2
rec3.seq = seq3
rec4.seq = seq4
rec5.seq = seq5
rec6.seq = seq6
try:
r[7] = (MultipleSeqAlignment(
[rec1, rec2, rec3, rec4, rec5,
rec6]).format('clustal'), None)
except ValueError:
print(hsp1)
r[7] = (None, None)
continue
return results
def test_two_identical_seqs_returns_two_ids_clustered(self):
alignment = MultipleSeqAlignment(
[SeqRecord(Seq("AAAT"), id="s1"), SeqRecord(Seq("AAAT"), id="s2"),]
)
result = AlignedSeq.kmeans_cluster_seqs_in_interval([0, 3], alignment, 1)
self.assertEqual(result, [["s1", "s2"]])