def iterator_sorted(gff_iterator, sort_order="gene"):
'''sort input and yield sorted output.'''
entries = list(gff_iterator)
if sort_order == "gene":
entries.sort(key=lambda x: (x.gene_id, x.contig, x.start))
elif sort_order == "gene+transcript":
entries.sort(
key=lambda x: (x.gene_id, x.transcript_id, x.contig, x.start))
elif sort_order == "contig+gene":
entries.sort(
key=lambda x: (x.contig, x.gene_id, x.transcript_id, x.start))
elif sort_order == "transcript":
entries.sort(key=lambda x: (x.transcript_id, x.contig, x.start))
elif sort_order == "position":
entries.sort(key=lambda x: (x.contig, x.start))
elif sort_order == "position+gene":
entries.sort(key=lambda x: (x.gene_id, x.start))
genes = list(flat_gene_iterator(entries))
genes.sort(key=lambda x: (x[0].contig, x[0].start))
entries = IOTools.flatten(genes)
elif sort_order == "gene+exon":
entries.sort(key=lambda x: (x.gene_id, x.exon_number))
for entry in entries:
yield entry
dbh = sqlite3.connect(PARAMS['database'])
return dbh
@transform(INPUT_FORMATS, regex("(.*)"), r"\1")
def unprocessReads(infiles, outfiles):
"""dummy task - no processing of reads."""
# if preprocess tools are specified, preprocessing is done on output that has
# already been generated in the first run
if PARAMS.get("preprocessors", None):
if PARAMS["auto_remove"]:
# check if fastqc has been run
for x in IOTools.flatten([glob.glob(y) for y in INPUT_FORMATS]):
f = re.match(REGEX_TRACK, x).group(1) + ".fastqc"
if not os.path.exists(f):
raise ValueError("file %s missing, "
"you need to run the pipeline once before "
"specifying 'auto_remove'" % f)
@follows(mkdir("fasta.dir"))
@transform(unprocessReads, regex(SEQUENCEFILES_REGEX),
r"fasta.dir/\1.fasta")
def makeAdaptorFasta(infile, outfile):
'''Make a single fasta file for each sample of all contaminant adaptor
sequences for removal
'''
print(infile)
def main(argv=None):
if argv is None:
argv = sys.argv
parser = E.OptionParser(version="%prog version: $Id$",
usage=globals()["__doc__"])
parser.add_option("-s",
"--ignore-strand",
dest="ignore_strand",
action="store_true",
help="ignore strand information [default=%default].")
parser.add_option(
"-u",
"--update",
dest="filename_update",
type="string",
help="if filename is given, previous results will be read"
"from there and only changed sets will be computed "
"[default=%default].")
parser.add_option("-p",
"--pattern-identifier",
dest="pattern_id",
type="string",
help="pattern to convert a filename to an id"
"[default=%default].")
parser.add_option("-g",
"--output-only-genes",
dest="output_only_genes",
action="store_true",
help="only output gene stats (includes gene lists)"
" [default=%default].")
parser.set_defaults(
ignore_strand=False,
filename_update=None,
pattern_id="(.*).gtf",
output_only_genes=False,
)
(options, args) = E.start(parser)
if len(args) < 2:
print(USAGE)
raise ValueError("at least two arguments required")
if options.filename_update:
infile = IOTools.open_file(options.filename_update, "r")
previous_results = {}
for line in infile:
if line.startswith("#"):
continue
if line.startswith("set1"):
continue
data = line[:-1].split("\t")
set1, set2 = data[0], data[1]
if set1 not in previous_results:
previous_results[set1] = {}
if set2 not in previous_results:
previous_results[set2] = {}
previous_results[set1][set2] = "\t".join(data[2:])
rev = [(data[x + 1], data[x]) for x in range(2, len(data), 2)]
previous_results[set2][set1] = "\t".join(IOTools.flatten(rev))
else:
previous_results = {}
if options.output_only_genes:
counter = CounterGenes()
else:
counter = Counter()
options.stdout.write("set1\tset2\t%s\n" % counter.getHeader())
pattern_id = re.compile(options.pattern_id)
def getTitle(x):
try:
return pattern_id.search(x).groups()[0]
except AttributeError:
return x
ncomputed, nupdated = 0, 0
for x in range(len(args)):
title1 = getTitle(args[x])
for y in range(0, x):
title2 = getTitle(args[y])
if previous_results:
try:
prev = previous_results[title1][title2]
except KeyError:
pass
else:
options.stdout.write("%s\t%s\t%s\n" %
((title1, title2, prev)))
nupdated += 1
continue
counter.count(args[x], args[y])
options.stdout.write("%s\t%s\t%s\n" %
((title1, title2, str(counter))))
ncomputed += 1
E.info("nupdated=%i, ncomputed=%i" % (nupdated, ncomputed))
E.stop()
def main(argv=None):
"""script main.
parses command line options in sys.argv, unless *argv* is given.
"""
if not argv:
argv = sys.argv
# setup command line parser
parser = E.OptionParser(
version="%prog version: $Id: diff_bed.py 2866 2010-03-03 10:18:49Z andreas $", usage=globals()["__doc__"])
parser.add_option("-u", "--update", dest="filename_update", type="string",
help="if filename is given, previous results will be read from there and only changed sets will be computed [default=%default].")
parser.add_option("-p", "--pattern-identifier", dest="pattern_id", type="string",
help="pattern to convert a filename to an id [default=%default].")
parser.add_option("-t", "--tracks", dest="tracks", action="store_true",
help="compare files against all tracks in the first file [default=%default]")
parser.set_defaults(
filename_update=None,
pattern_id="(.*).bed",
tracks=None,
)
# add common options (-h/--help, ...) and parse command line
(options, args) = E.start(parser, argv=argv)
if len(args) < 2:
raise ValueError("at least two arguments required")
if options.filename_update:
infile = IOTools.open_file(options.filename_update, "r")
previous_results = {}
for line in infile:
if line.startswith("#"):
continue
if line.startswith("set1"):
continue
data = line[:-1].split("\t")
set1, set2 = data[0], data[1]
if set1 not in previous_results:
previous_results[set1] = {}
if set2 not in previous_results:
previous_results[set2] = {}
previous_results[set1][set2] = "\t".join(data[2:])
rev = [(data[x + 1], data[x]) for x in range(2, len(data), 2)]
previous_results[set2][set1] = "\t".join(IOTools.flatten(rev))
else:
previous_results = {}
pattern_id = re.compile(options.pattern_id)
def getTitle(x):
try:
return pattern_id.search(x).groups()[0]
except AttributeError:
return x
ncomputed, nupdated = 0, 0
if options.tracks:
counter = CounterTracks(args[0])
options.stdout.write("set1\tset2\t%s\n" % counter.getHeader())
for filename in args[1:]:
title1 = getTitle(filename)
for title2 in counter.getTracks():
if previous_results:
try:
prev = previous_results[title1][title2]
except KeyError:
pass
else:
options.stdout.write(
"%s\t%s\t%s\n" % ((title1, title2, prev)))
nupdated += 1
continue
counter.count(filename, title2)
options.stdout.write(
"%s\t%s\t%s\n" % ((title1, title2, str(counter))))
ncomputed += 1
else:
counter = Counter()
options.stdout.write("set1\tset2\t%s\n" % counter.getHeader())
for x in range(len(args)):
title1 = getTitle(args[x])
for y in range(0, x):
title2 = getTitle(args[y])
if previous_results:
try:
prev = previous_results[title1][title2]
except KeyError:
pass
else:
options.stdout.write(
"%s\t%s\t%s\n" % ((title1, title2, prev)))
nupdated += 1
continue
counter.count(args[x], args[y])
options.stdout.write(
"%s\t%s\t%s\n" % ((title1, title2, str(counter))))
ncomputed += 1
E.info("nupdated=%i, ncomputed=%i" % (nupdated, ncomputed))
E.stop()