def finish(self):
self.e.lihahome()
worklist.userprompt(
"Process complete. Continue to turn off reagent cooler")
self.e.setreagenttemp(None)
#Sample.printallsamples("At completion")
hasError = False
for s in Sample.getAllOnPlate():
if s.volume < 1.0 and s.conc is not None and not s.hasBeads:
print "ERROR: Insufficient volume for ", s, " need at least ", 1.0 - s.volume, " ul additional"
#hasError=True
elif s.volume < 2.5 and s.conc is not None:
print "WARNING: Low final volume for ", s
elif s.volume > s.plate.maxVolume:
print "ERROR: Excess final volume (", s.volume, ") for ", s, ", maximum is ", s.plate.maxVolume
hasError = True
if hasError:
print "NO OUTPUT DUE TO ERRORS"
assert (False)
print "Wells used: samples: %d, dilutions: %d, qPCR: %d" % (
Sample.numSamplesOnPlate(decklayout.SAMPLEPLATE),
Sample.numSamplesOnPlate(decklayout.DILPLATE),
Sample.numSamplesOnPlate(decklayout.QPCRPLATE))
# Save worklist to a file
#e.saveworklist("trp1.gwl")
(scriptname, ext) = os.path.splitext(sys.argv[0])
self.e.savegem(scriptname + ".gem")
self.e.savesummary(scriptname + ".txt")
Sample.savematlab(scriptname + ".m")
def finish(self):
self.e.lihahome()
worklist.userprompt("Process complete. Continue to turn off reagent cooler")
self.e.setreagenttemp(None)
#Sample.printallsamples("At completion")
hasError=False
for s in Sample.getAllOnPlate():
if s.volume<1.0 and s.conc is not None and not s.hasBeads:
print "ERROR: Insufficient volume for ", s," need at least ",1.0-s.volume," ul additional"
#hasError=True
elif s.volume<2.5 and s.conc is not None:
print "WARNING: Low final volume for ", s
elif s.volume>s.plate.maxVolume:
print "ERROR: Excess final volume (",s.volume,") for ",s,", maximum is ",s.plate.maxVolume
hasError=True
if hasError:
print "NO OUTPUT DUE TO ERRORS"
assert(False)
print "Wells used: samples: %d, dilutions: %d, qPCR: %d"%(Sample.numSamplesOnPlate(decklayout.SAMPLEPLATE),Sample.numSamplesOnPlate(decklayout.DILPLATE),Sample.numSamplesOnPlate(decklayout.QPCRPLATE))
# Save worklist to a file
#e.saveworklist("trp1.gwl")
(scriptname,ext)=os.path.splitext(sys.argv[0])
self.e.savegem(scriptname+".gem")
self.e.savesummary(scriptname+".txt")
Sample.savematlab(scriptname+".m")
def finish(self):
self.e.lihahome()
worklist.userprompt("Process complete. Continue to turn off reagent cooler")
self.e.setreagenttemp(None)
#Sample.printallsamples("At completion")
hasError=False
for s in Sample.getAllOnPlate():
if s.volume<1.0 and s.conc is not None and not s.emptied:
logging.error("Insufficient volume for %s: need at least %.1f ul additional"%(s.name,1.0-s.volume),fatal=False)
#hasError=True
elif s.volume<2.5 and s.conc is not None and not s.emptied:
logging.warning("Low final volume for "+ s.name)
elif s.volume>s.plate.maxVolume:
logging.erorr("Excess final volume (%.1f) for %s: maximum is %.1f ul"%(s.volume,s.name,s.plate.maxVolume),fatal=False)
hasError=True
if hasError:
logging.error("NO OUTPUT DUE TO ERRORS")
print "Wells used: samples: %d, dilutions: %d, qPCR: %d"%(Sample.numSamplesOnPlate(decklayout.SAMPLEPLATE),Sample.numSamplesOnPlate(decklayout.DILPLATE),Sample.numSamplesOnPlate(decklayout.QPCRPLATE))
# Save worklist to a file
#e.saveworklist("trp1.gwl")
(scriptname,ext)=os.path.splitext(sys.argv[0])
self.e.savegem(scriptname+".gem")
self.e.savesummary(scriptname+".txt")
Sample.savematlab(scriptname+".m")
