def create_voltage_signal(self, list_of_rois):
filled_mask = OriginalImage = np.zeros((1000, 1000))
for roi in list_of_rois:
filled_mask += polygon2mask((1000, 1000), (roi + 5) * 100)
filled_mask = (filled_mask > 0).astype(int).transpose()
fig, axs = plt.subplots(1, 1)
axs.imshow(filled_mask)
scanning_voltage = 5
points_per_contour = int(self.points_per_contour_textbox.text())
sampling_rate = int(self.sampling_rate_textbox.text())
contourScanningSignal = ProcessImage.mask_to_contourScanning_DAQsignals(
filled_mask,
OriginalImage,
scanning_voltage,
points_per_contour,
sampling_rate,
repeats=1)
contourScanningSignal = np.vstack(
(contourScanningSignal[0][0], contourScanningSignal[1][0]))
self.galvoThread = pmtimagingTest_contour()
self.galvoThread.setWave_contourscan(sampling_rate,
contourScanningSignal,
points_per_contour)
def __init__(self, *args, **kwargs):
super().__init__(*args, **kwargs)
# os.chdir('./')# Set directory to current folder.
self.setFont(QFont("Arial"))
self.setMinimumSize(1200,850)
self.setWindowTitle("PMTWidget")
self.layout = QGridLayout(self)
#------------------------Initiating class-------------------
self.pmtTest = pmtimagingTest()
self.pmtTest_contour = pmtimagingTest_contour()
self.savedirectory = r'M:\tnw\ist\do\projects\Neurophotonics\Brinkslab\Data\Octoscope\pmt_image_default_dump'
self.prefixtextboxtext = '_fromGalvoWidget'
#**************************************************************************************************************************************
#--------------------------------------------------------------------------------------------------------------------------------------
#-----------------------------------------------------------GUI for PMT tab------------------------------------------------------------
#--------------------------------------------------------------------------------------------------------------------------------------
#**************************************************************************************************************************************
pmtimageContainer = QGroupBox("PMT image")
self.pmtimageLayout = QGridLayout()
self.pmtvideoWidget = pg.ImageView()
self.pmtvideoWidget.ui.roiBtn.hide()
self.pmtvideoWidget.ui.menuBtn.hide()
self.pmtvideoWidget.resize(400,400)
self.pmtimageLayout.addWidget(self.pmtvideoWidget, 0, 0)
pmtroiContainer = QGroupBox("PMT ROI")
self.pmtimageroiLayout = QGridLayout()
self.pmt_roiwidget = pg.GraphicsLayoutWidget()
self.pmt_roiwidget.resize(150,150)
self.pmt_roiwidget.addLabel('ROI', row=0, col=0)
# create ROI
self.vb_2 = self.pmt_roiwidget.addViewBox(row=1, col=0, lockAspect=True, colspan=1)
self.vb_2.name = 'ROI'
self.pmtimgroi = pg.ImageItem()
self.vb_2.addItem(self.pmtimgroi)
#self.roi = pg.RectROI([20, 20], [20, 20], pen=(0,9))
#r1 = QRectF(0, 0, 895, 500)
ROIpen = QPen() # creates a default pen
ROIpen.setStyle(Qt.DashDotLine)
ROIpen.setWidth(0.5)
ROIpen.setBrush(QColor(0,161,255))
self.roi = pg.PolyLineROI([[0,0], [80,0], [80,80], [0,80]], closed=True, pen=ROIpen)#, maxBounds=r1
#self.roi.addScaleHandle([1,0], [1, 0])
self.roi.sigHoverEvent.connect(lambda: self.show_handle_num()) # update handle numbers
self.pmtvb = self.pmtvideoWidget.getView()
self.pmtimageitem = self.pmtvideoWidget.getImageItem()
self.pmtvb.addItem(self.roi)# add ROIs to main image
self.pmtimageroiLayout.addWidget(self.pmt_roiwidget, 0, 0)
pmtimageContainer.setMinimumWidth(850)
pmtroiContainer.setMaximumHeight(380)
# pmtroiContainer.setMaximumWidth(300)
pmtimageContainer.setLayout(self.pmtimageLayout)
pmtroiContainer.setLayout(self.pmtimageroiLayout)
#----------------------------Contour-----------------------------------
pmtContourContainer = QGroupBox("Contour selection")
pmtContourContainer.setFixedWidth(280)
self.pmtContourLayout = QGridLayout()
#contour_Description = QLabel("Handle number updates when parking mouse cursor upon ROI. Points in contour are divided evenly between handles.")
#contour_Description.setStyleSheet('color: blue')
#self.pmtContourLayout.addWidget(contour_Description,0,0)
self.pmt_handlenum_Label = QLabel("Handle number: ")
self.pmtContourLayout.addWidget(self.pmt_handlenum_Label,1,0)
self.contour_strategy = QComboBox()
self.contour_strategy.addItems(['Manual','Uniform'])
self.pmtContourLayout.addWidget(self.contour_strategy, 1, 1)
self.pointsinContour = QSpinBox(self)
self.pointsinContour.setMinimum(1)
self.pointsinContour.setMaximum(1000)
self.pointsinContour.setValue(100)
self.pointsinContour.setSingleStep(100)
self.pmtContourLayout.addWidget(self.pointsinContour, 2, 1)
self.pmtContourLayout.addWidget(QLabel("Points in contour:"), 2, 0)
self.contour_samprate = QSpinBox(self)
self.contour_samprate.setMinimum(0)
self.contour_samprate.setMaximum(1000000)
self.contour_samprate.setValue(50000)
self.contour_samprate.setSingleStep(50000)
self.pmtContourLayout.addWidget(self.contour_samprate, 3, 1)
self.pmtContourLayout.addWidget(QLabel("Sampling rate:"), 3, 0)
self.generate_contour_sacn = StylishQT.generateButton()
self.pmtContourLayout.addWidget(self.generate_contour_sacn, 4, 1)
self.generate_contour_sacn.clicked.connect(lambda: self.generate_contour())
self.do_contour_sacn = StylishQT.runButton("Contour")
self.do_contour_sacn.setFixedHeight(32)
self.pmtContourLayout.addWidget(self.do_contour_sacn, 5, 0)
self.do_contour_sacn.clicked.connect(lambda:self.buttonenabled('contourscan', 'start'))
self.do_contour_sacn.clicked.connect(lambda: self.measure_pmt_contourscan())
self.stopButton_contour = StylishQT.stop_deleteButton()
self.stopButton_contour.setFixedHeight(32)
self.stopButton_contour.clicked.connect(lambda:self.buttonenabled('contourscan', 'stop'))
self.stopButton_contour.clicked.connect(lambda: self.stopMeasurement_pmt_contour())
self.stopButton_contour.setEnabled(False)
self.pmtContourLayout.addWidget(self.stopButton_contour, 5, 1)
pmtContourContainer.setLayout(self.pmtContourLayout)
#----------------------------Control-----------------------------------
controlContainer = QGroupBox("Galvo Scanning Panel")
controlContainer.setFixedWidth(280)
self.controlLayout = QGridLayout()
self.pmt_fps_Label = QLabel("Per frame: ")
self.controlLayout.addWidget(self.pmt_fps_Label, 5, 0)
self.saveButton_pmt = StylishQT.saveButton()
self.saveButton_pmt.clicked.connect(lambda: self.saveimage_pmt())
self.controlLayout.addWidget(self.saveButton_pmt, 5, 1)
self.startButton_pmt = StylishQT.runButton("")
self.startButton_pmt.setFixedHeight(32)
self.startButton_pmt.setCheckable(True)
self.startButton_pmt.clicked.connect(lambda:self.buttonenabled('rasterscan', 'start'))
self.startButton_pmt.clicked.connect(lambda: self.measure_pmt())
self.controlLayout.addWidget(self.startButton_pmt, 6, 0)
self.stopButton = StylishQT.stop_deleteButton()
self.stopButton.setFixedHeight(32)
self.stopButton.clicked.connect(lambda:self.buttonenabled('rasterscan', 'stop'))
self.stopButton.clicked.connect(lambda: self.stopMeasurement_pmt())
self.stopButton.setEnabled(False)
self.controlLayout.addWidget(self.stopButton, 6, 1)
#-----------------------------------Galvo scanning------------------------------------------------------------------------
self.textboxAA_pmt = QSpinBox(self)
self.textboxAA_pmt.setMinimum(0)
self.textboxAA_pmt.setMaximum(1000000)
self.textboxAA_pmt.setValue(500000)
self.textboxAA_pmt.setSingleStep(100000)
self.controlLayout.addWidget(self.textboxAA_pmt, 1, 1)
self.controlLayout.addWidget(QLabel("Sampling rate:"), 1, 0)
#self.controlLayout.addWidget(QLabel("Galvo raster scanning : "), 1, 0)
self.textbox1B_pmt = QSpinBox(self)
self.textbox1B_pmt.setMinimum(-10)
self.textbox1B_pmt.setMaximum(10)
self.textbox1B_pmt.setValue(3)
self.textbox1B_pmt.setSingleStep(1)
self.controlLayout.addWidget(self.textbox1B_pmt, 2, 1)
self.controlLayout.addWidget(QLabel("Volt range:"), 2, 0)
self.Scanning_pixel_num_combobox = QSpinBox(self)
self.Scanning_pixel_num_combobox.setMinimum(0)
self.Scanning_pixel_num_combobox.setMaximum(1000)
self.Scanning_pixel_num_combobox.setValue(500)
self.Scanning_pixel_num_combobox.setSingleStep(244)
self.controlLayout.addWidget(self.Scanning_pixel_num_combobox, 3, 1)
self.controlLayout.addWidget(QLabel("Pixel number:"), 3, 0)
self.textbox1H_pmt = QSpinBox(self)
self.textbox1H_pmt.setMinimum(1)
self.textbox1H_pmt.setMaximum(20)
self.textbox1H_pmt.setValue(1)
self.textbox1H_pmt.setSingleStep(1)
self.controlLayout.addWidget(self.textbox1H_pmt, 4, 1)
self.controlLayout.addWidget(QLabel("average over:"), 4, 0)
controlContainer.setLayout(self.controlLayout)
#---------------------------Set tab1 layout---------------------------
# pmtmaster = QGridLayout()
self.layout.addWidget(pmtimageContainer, 0,0,3,1)
self.layout.addWidget(pmtroiContainer,1,1)
self.layout.addWidget(pmtContourContainer,2,1)
self.layout.addWidget(controlContainer,0,1)
def __init__(self, *args, **kwargs):
super().__init__(*args, **kwargs)
os.chdir(os.path.dirname(
sys.argv[0])) # Set directory to current folder.
self.setWindowIcon(QIcon('./Icons/Icon.png'))
self.setFont(QFont("Arial"))
# print(str(os.getcwd())+'Tupo')
# sys.stdout = EmittingStream(textWritten = self.normalOutputWritten) # Uncomment here to link console output to textedit.
# sys.stdout = sys.__stdout__
#------------------------Initiating patchclamp class-------------------
self.pmtTest = pmtimagingTest()
self.pmtTest_contour = pmtimagingTest_contour()
self.OC = 0.1
#----------------------------------------------------------------------
#----------------------------------GUI---------------------------------
#----------------------------------------------------------------------
self.setMinimumSize(1710, 1200)
self.setWindowTitle("Tupolev v1.0")
self.layout = QGridLayout(self)
# Setting tabs
self.tabs = QTabWidget()
self.Galvo_WidgetInstance = GalvoWidget.PMTWidget.PMTWidgetUI()
self.Waveformer_WidgetInstance = NIDAQ.Waveformer_for_screening.WaveformGenerator(
)
self.PatchClamp_WidgetInstance = PatchClamp.ui_patchclamp_sealtest.PatchclampSealTestUI(
)
#self.tab4 = ui_camera_lab_5.CameraUI()
self.Analysis_WidgetInstance = ImageAnalysis.AnalysisWidget.AnalysisWidgetUI(
)
# Add tabs
self.tabs.addTab(self.Galvo_WidgetInstance, "PMT imaging")
self.tabs.addTab(self.Waveformer_WidgetInstance, "Waveform")
self.tabs.addTab(self.PatchClamp_WidgetInstance, "Patch clamp")
#self.tabs.addTab(self.tab4,"Camera")
self.tabs.addTab(self.Analysis_WidgetInstance, "Image analysis")
self.savedirectory = os.path.join(
os.path.expanduser("~"),
"Desktop") #'M:/tnw/ist/do/projects/Neurophotonics/Brinkslab/Data'
# Establishing communication betweeb widgets.
self.Galvo_WidgetInstance.SignalForContourScanning.connect(
self.PassVariable_GalvoWidget2Waveformer)
self.Galvo_WidgetInstance.MessageBack.connect(self.normalOutputWritten)
self.Analysis_WidgetInstance.MessageBack.connect(
self.normalOutputWritten)
#**************************************************************************************************************************************
#--------------------------------------------------------------------------------------------------------------------------------------
#-----------------------------------------------------------GUI for set directory------------------------------------------------------
#--------------------------------------------------------------------------------------------------------------------------------------
#**************************************************************************************************************************************
setdirectoryContainer = QGroupBox("Set directory")
self.setdirectorycontrolLayout = QGridLayout()
self.saving_prefix = ''
self.savedirectorytextbox = QLineEdit(self)
self.savedirectorytextbox.setPlaceholderText('Saving directory')
self.setdirectorycontrolLayout.addWidget(self.savedirectorytextbox, 0,
1)
self.prefixtextbox = QLineEdit(self)
self.prefixtextbox.setPlaceholderText('Prefix')
self.setdirectorycontrolLayout.addWidget(self.prefixtextbox, 0, 0)
#self.setdirectorycontrolLayout.addWidget(QLabel("Saving prefix:"), 0, 0)
self.toolButtonOpenDialog = QtWidgets.QPushButton('Click me!')
self.toolButtonOpenDialog.setStyleSheet(
"QPushButton {color:teal;background-color: pink; border-style: outset;border-radius: 5px;border-width: 2px;font: bold 14px;padding: 2px}"
"QPushButton:pressed {color:yellow;background-color: pink; border-style: outset;border-radius: 5px;border-width: 2px;font: bold 14px;padding: 2px}"
)
self.toolButtonOpenDialog.setObjectName("toolButtonOpenDialog")
self.toolButtonOpenDialog.clicked.connect(self._open_file_dialog)
self.setdirectorycontrolLayout.addWidget(self.toolButtonOpenDialog, 0,
2)
setdirectoryContainer.setLayout(self.setdirectorycontrolLayout)
setdirectoryContainer.setMaximumHeight(70)
self.layout.addWidget(setdirectoryContainer, 0, 0)
#**************************************************************************************************************************************
#--------------------------------------------------------------------------------------------------------------------------------------
#-----------------------------------------------------------GUI for AOTF---------------------------------------------------------------
#--------------------------------------------------------------------------------------------------------------------------------------
#**************************************************************************************************************************************
AOTFcontrolContainer = QGroupBox("AOTF control")
self.AOTFcontrolLayout = QGridLayout()
self.slider640 = QSlider(Qt.Horizontal)
self.slider640.setMinimum(0)
self.slider640.setMaximum(500)
self.slider640.setTickPosition(QSlider.TicksBothSides)
self.slider640.setTickInterval(100)
self.slider640.setSingleStep(1)
self.line640 = QLineEdit(self)
self.line640.setFixedWidth(60)
self.slider640.sliderReleased.connect(
lambda: self.updatelinevalue(640))
self.slider640.sliderReleased.connect(
lambda: self.execute_tread_single_sample_analog('640AO'))
self.line640.returnPressed.connect(lambda: self.updatesider(640))
self.ICON_off_AOTF = "./Icons/AOTF_off.png"
self.AOTF_red_iconlabel = QLabel(self)
self.AOTF_red_iconlabel.setPixmap(QPixmap(self.ICON_off_AOTF))
self.AOTFcontrolLayout.addWidget(self.AOTF_red_iconlabel, 0, 0)
self.switchbutton_640 = QPushButton("640")
self.switchbutton_640.setStyleSheet(
"QPushButton {color:white;background-color: red; border-style: outset;border-radius: 10px;border-width: 2px;font: bold 14px;padding: 6px}"
"QPushButton:pressed {color:black;background-color: red; border-style: outset;border-radius: 10px;border-width: 2px;font: bold 14px;padding: 6px}"
)
self.switchbutton_640.setCheckable(True)
#self.holdingbutton.toggle()
self.switchbutton_640.clicked.connect(
lambda: self.execute_tread_single_sample_digital('640blanking'))
self.switchbutton_640.clicked.connect(
lambda: self.change_AOTF_icon('640blanking'))
self.AOTFcontrolLayout.addWidget(self.switchbutton_640, 0, 1)
self.slider532 = QSlider(Qt.Horizontal)
self.slider532.setMinimum(0)
self.slider532.setMaximum(500)
self.slider532.setTickPosition(QSlider.TicksBothSides)
self.slider532.setTickInterval(100)
self.slider532.setSingleStep(1)
self.line532 = QLineEdit(self)
self.line532.setFixedWidth(60)
self.slider532.sliderReleased.connect(
lambda: self.updatelinevalue(532))
self.slider532.sliderReleased.connect(
lambda: self.execute_tread_single_sample_analog('532AO'))
self.line532.returnPressed.connect(lambda: self.updatesider(532))
self.AOTF_green_iconlabel = QLabel(self)
self.AOTF_green_iconlabel.setPixmap(QPixmap(self.ICON_off_AOTF))
self.AOTFcontrolLayout.addWidget(self.AOTF_green_iconlabel, 1, 0)
self.switchbutton_532 = QPushButton("532")
self.switchbutton_532.setStyleSheet(
"QPushButton {color:white;background-color: green; border-style: outset;border-radius: 10px;border-width: 2px;font: bold 14px;padding: 6px}"
"QPushButton:pressed {color:black;background-color: green; border-style: outset;border-radius: 10px;border-width: 2px;font: bold 14px;padding: 6px}"
)
self.switchbutton_532.setCheckable(True)
#self.holdingbutton.toggle()
self.switchbutton_532.clicked.connect(
lambda: self.execute_tread_single_sample_digital('532blanking'))
self.switchbutton_532.clicked.connect(
lambda: self.change_AOTF_icon('532blanking'))
self.AOTFcontrolLayout.addWidget(self.switchbutton_532, 1, 1)
self.slider488 = QSlider(Qt.Horizontal)
self.slider488.setMinimum(0)
self.slider488.setMaximum(500)
self.slider488.setTickPosition(QSlider.TicksBothSides)
self.slider488.setTickInterval(100)
self.slider488.setSingleStep(1)
self.line488 = QLineEdit(self)
self.line488.setFixedWidth(60)
self.slider488.sliderReleased.connect(
lambda: self.updatelinevalue(488))
self.slider488.sliderReleased.connect(
lambda: self.execute_tread_single_sample_analog('488AO'))
self.line488.returnPressed.connect(lambda: self.updatesider(488))
self.AOTF_blue_iconlabel = QLabel(self)
self.AOTF_blue_iconlabel.setPixmap(QPixmap(self.ICON_off_AOTF))
self.AOTFcontrolLayout.addWidget(self.AOTF_blue_iconlabel, 2, 0)
self.switchbutton_488 = QPushButton("488")
self.switchbutton_488.setStyleSheet(
"QPushButton {color:white;background-color: blue; border-style: outset;border-radius: 10px;border-width: 2px;font: bold 14px;padding: 6px}"
"QPushButton:pressed {color:black;background-color: blue; border-style: outset;border-radius: 10px;border-width: 2px;font: bold 14px;padding: 6px}"
)
self.switchbutton_488.setCheckable(True)
#self.holdingbutton.toggle()
self.switchbutton_488.clicked.connect(
lambda: self.execute_tread_single_sample_digital('488blanking'))
self.switchbutton_488.clicked.connect(
lambda: self.change_AOTF_icon('488blanking'))
self.AOTFcontrolLayout.addWidget(self.switchbutton_488, 2, 1)
self.AOTFcontrolLayout.addWidget(self.slider640, 0, 2)
self.AOTFcontrolLayout.addWidget(self.line640, 0, 3)
self.AOTFcontrolLayout.addWidget(self.slider532, 1, 2)
self.AOTFcontrolLayout.addWidget(self.line532, 1, 3)
self.AOTFcontrolLayout.addWidget(self.slider488, 2, 2)
self.AOTFcontrolLayout.addWidget(self.line488, 2, 3)
AOTFcontrolContainer.setLayout(self.AOTFcontrolLayout)
AOTFcontrolContainer.setMaximumHeight(300)
self.layout.addWidget(AOTFcontrolContainer, 1, 0)
#**************************************************************************************************************************************
#--------------------------------------------------------------------------------------------------------------------------------------
#-----------------------------------------------------------GUI for Stage--------------------------------------------------------------
#--------------------------------------------------------------------------------------------------------------------------------------
#**************************************************************************************************************************************
stagecontrolContainer = QGroupBox("Stage control")
self.stagecontrolLayout = QGridLayout()
self.stage_upwards = QPushButton("↑")
self.stage_upwards.setStyleSheet(
"QPushButton {color:white;background-color: Olive; border-style: outset;border-radius: 10px;border-width: 2px;font: bold 14px;padding: 6px}"
"QPushButton:pressed {color:red;background-color: white; border-style: outset;border-radius: 10px;border-width: 2px;font: bold 14px;padding: 6px}"
)
self.stagecontrolLayout.addWidget(self.stage_upwards, 1, 2)
self.stage_upwards.clicked.connect(
lambda: self.sample_stage_move_upwards())
self.stage_upwards.setShortcut('w')
self.stage_left = QPushButton("←")
self.stage_left.setStyleSheet(
"QPushButton {color:white;background-color: Olive; border-style: outset;border-radius: 10px;border-width: 2px;font: bold 14px;padding: 6px}"
"QPushButton:pressed {color:red;background-color: white; border-style: outset;border-radius: 10px;border-width: 2px;font: bold 14px;padding: 6px}"
)
self.stagecontrolLayout.addWidget(self.stage_left, 2, 1)
self.stage_left.clicked.connect(
lambda: self.sample_stage_move_leftwards())
self.stage_left.setShortcut('a')
self.stage_right = QPushButton("→")
self.stage_right.setStyleSheet(
"QPushButton {color:white;background-color: Olive; border-style: outset;border-radius: 10px;border-width: 2px;font: bold 14px;padding: 6px}"
"QPushButton:pressed {color:red;background-color: white; border-style: outset;border-radius: 10px;border-width: 2px;font: bold 14px;padding: 6px}"
)
self.stagecontrolLayout.addWidget(self.stage_right, 2, 3)
self.stage_right.clicked.connect(
lambda: self.sample_stage_move_rightwards())
self.stage_right.setShortcut('d')
self.stage_down = QPushButton("↓")
self.stage_down.setStyleSheet(
"QPushButton {color:white;background-color: Olive; border-style: outset;border-radius: 10px;border-width: 2px;font: bold 14px;padding: 6px}"
"QPushButton:pressed {color:red;background-color: white; border-style: outset;border-radius: 10px;border-width: 2px;font: bold 14px;padding: 6px}"
)
self.stagecontrolLayout.addWidget(self.stage_down, 2, 2)
self.stage_down.clicked.connect(
lambda: self.sample_stage_move_downwards())
self.stage_down.setShortcut('s')
self.stage_speed = QSpinBox(self)
self.stage_speed.setMinimum(-10000)
self.stage_speed.setMaximum(10000)
self.stage_speed.setValue(300)
self.stage_speed.setSingleStep(100)
self.stagecontrolLayout.addWidget(self.stage_speed, 0, 1)
self.stagecontrolLayout.addWidget(QLabel("Moving speed:"), 0, 0)
self.led_Label = QLabel("White LED: ")
self.stagecontrolLayout.addWidget(self.led_Label, 0, 2)
self.switchbutton_LED = QPushButton()
#self.switchbutton_LED.setStyleSheet("QPushButton {color:white;background-color: blue; border-style: outset;border-radius: 10px;border-width: 2px;font: bold 14px;padding: 6px}"
#"QPushButton:pressed {color:black;background-color: blue; border-style: outset;border-radius: 10px;border-width: 2px;font: bold 14px;padding: 6px}")
self.switchbutton_LED.setCheckable(True)
self.switchbutton_LED.setIcon(QIcon('./Icons/AOTF_off.png'))
#self.holdingbutton.toggle()
self.switchbutton_LED.clicked.connect(
lambda: self.execute_tread_single_sample_digital('LED'))
self.switchbutton_LED.clicked.connect(
lambda: self.change_AOTF_icon('LED'))
self.stagecontrolLayout.addWidget(self.switchbutton_LED, 0, 3)
self.stage_current_pos_Label = QLabel("Current position: ")
self.stagecontrolLayout.addWidget(self.stage_current_pos_Label, 1, 0)
self.stage_goto = QPushButton("Move to:")
self.stage_goto.setStyleSheet(
"QPushButton {color:white;background-color: blue; border-style: outset;border-radius: 10px;border-width: 2px;font: bold 14px;padding: 6px}"
"QPushButton:pressed {color:red;background-color: white; border-style: outset;border-radius: 10px;border-width: 2px;font: bold 14px;padding: 6px}"
)
self.stagecontrolLayout.addWidget(self.stage_goto, 3, 0)
self.stage_goto.clicked.connect(
lambda: self.sample_stage_move_towards())
self.stage_goto_x = QLineEdit(self)
self.stage_goto_x.setFixedWidth(60)
self.stagecontrolLayout.addWidget(self.stage_goto_x, 3, 1)
self.stage_goto_y = QLineEdit(self)
self.stage_goto_y.setFixedWidth(60)
self.stagecontrolLayout.addWidget(self.stage_goto_y, 3, 2)
self.stagecontrolLayout.addWidget(
QLabel('Click arrow to enable WASD keyboard control'), 4, 0, 1, 3)
stagecontrolContainer.setLayout(self.stagecontrolLayout)
stagecontrolContainer.setMaximumHeight(300)
self.layout.addWidget(stagecontrolContainer, 2, 0)
#**************************************************************************************************************************************
#--------------------------------------------------------------------------------------------------------------------------------------
#-----------------------------------------------------------GUI for Filter movement----------------------------------------------------
#--------------------------------------------------------------------------------------------------------------------------------------
#**************************************************************************************************************************************
ND_filtercontrolContainer = QGroupBox("ND filter control")
self.NDfiltercontrolLayout = QGridLayout()
bGBackupFromIntExt = QButtonGroup(self)
self.filter1_pos0 = QPushButton('0')
self.filter1_pos0.setCheckable(True)
bGBackupFromIntExt.addButton(self.filter1_pos0)
self.NDfiltercontrolLayout.addWidget(self.filter1_pos0, 0, 1)
self.filter1_pos0.clicked.connect(
lambda: self.filter_move_towards("COM9", 0))
self.filter1_pos1 = QPushButton('1')
self.filter1_pos1.setCheckable(True)
bGBackupFromIntExt.addButton(self.filter1_pos1)
self.NDfiltercontrolLayout.addWidget(self.filter1_pos1, 0, 2)
self.filter1_pos1.clicked.connect(
lambda: self.filter_move_towards("COM9", 1))
self.filter1_pos2 = QPushButton('2')
self.filter1_pos2.setCheckable(True)
bGBackupFromIntExt.addButton(self.filter1_pos2)
self.NDfiltercontrolLayout.addWidget(self.filter1_pos2, 0, 3)
self.filter1_pos2.clicked.connect(
lambda: self.filter_move_towards("COM9", 2))
self.filter1_pos3 = QPushButton('3')
self.filter1_pos3.setCheckable(True)
bGBackupFromIntExt.addButton(self.filter1_pos3)
self.NDfiltercontrolLayout.addWidget(self.filter1_pos3, 0, 4)
self.filter1_pos3.clicked.connect(
lambda: self.filter_move_towards("COM9", 3))
self.NDfiltercontrolLayout.addWidget(QLabel('Filter-1 pos: '), 0, 0)
self.NDfiltercontrolLayout.addWidget(QLabel('Filter-2 pos: '), 1, 0)
bGBackupFromIntExt_1 = QButtonGroup(self)
self.filter2_pos0 = QPushButton('0')
self.filter2_pos0.setCheckable(True)
bGBackupFromIntExt_1.addButton(self.filter2_pos0)
self.NDfiltercontrolLayout.addWidget(self.filter2_pos0, 1, 1)
self.filter2_pos0.clicked.connect(
lambda: self.filter_move_towards("COM7", 0))
self.filter2_pos1 = QPushButton('0.1')
self.filter2_pos1.setCheckable(True)
bGBackupFromIntExt_1.addButton(self.filter2_pos1)
self.NDfiltercontrolLayout.addWidget(self.filter2_pos1, 1, 2)
self.filter2_pos1.clicked.connect(
lambda: self.filter_move_towards("COM7", 1))
self.filter2_pos2 = QPushButton('0.3')
self.filter2_pos2.setCheckable(True)
bGBackupFromIntExt_1.addButton(self.filter2_pos2)
self.NDfiltercontrolLayout.addWidget(self.filter2_pos2, 1, 3)
self.filter2_pos2.clicked.connect(
lambda: self.filter_move_towards("COM7", 2))
self.filter2_pos3 = QPushButton('0.5')
self.filter2_pos3.setCheckable(True)
bGBackupFromIntExt_1.addButton(self.filter2_pos3)
self.NDfiltercontrolLayout.addWidget(self.filter2_pos3, 1, 4)
self.filter2_pos3.clicked.connect(
lambda: self.filter_move_towards("COM7", 3))
ND_filtercontrolContainer.setLayout(self.NDfiltercontrolLayout)
ND_filtercontrolContainer.setMaximumHeight(200)
self.layout.addWidget(ND_filtercontrolContainer, 3, 0)
#**************************************************************************************************************************************
#--------------------------------------------------------------------------------------------------------------------------------------
#-----------------------------------------------------------GUI for Objective Motor----------------------------------------------------
#--------------------------------------------------------------------------------------------------------------------------------------
#**************************************************************************************************************************************
# Movement based on relative positions.
ObjMotorcontrolContainer = QGroupBox("Objective motor control")
self.ObjMotorcontrolLayout = QGridLayout()
self.ObjMotor_connect = QPushButton("Connect")
self.ObjMotor_connect.setStyleSheet(
"QPushButton {color:white;background-color: green; border-style: outset;border-radius: 10px;border-width: 2px;font: bold 14px;padding: 6px}"
"QPushButton:pressed {color:red;background-color: white; border-style: outset;border-radius: 10px;border-width: 2px;font: bold 14px;padding: 6px}"
)
self.ObjMotorcontrolLayout.addWidget(self.ObjMotor_connect, 0, 0)
self.ObjMotor_connect.clicked.connect(lambda: self.ConnectMotor())
self.ObjMotor_disconnect = QPushButton("Disconnect")
self.ObjMotor_disconnect.setStyleSheet(
"QPushButton {color:white;background-color: firebrick; border-style: outset;border-radius: 10px;border-width: 2px;font: bold 14px;padding: 6px}"
"QPushButton:pressed {color:red;background-color: white; border-style: outset;border-radius: 10px;border-width: 2px;font: bold 14px;padding: 6px}"
)
self.ObjMotorcontrolLayout.addWidget(self.ObjMotor_disconnect, 0, 1)
self.ObjMotor_disconnect.clicked.connect(
lambda: self.DisconnectMotor())
self.ObjMotor_upwards = QPushButton("↑")
self.ObjMotor_upwards.setStyleSheet(
"QPushButton {color:white;background-color: teal; border-style: outset;border-radius: 10px;border-width: 2px;font: bold 14px;padding: 6px}"
"QPushButton:pressed {color:red;background-color: white; border-style: outset;border-radius: 10px;border-width: 2px;font: bold 14px;padding: 6px}"
)
self.ObjMotorcontrolLayout.addWidget(self.ObjMotor_upwards, 2, 2)
self.ObjMotor_upwards.clicked.connect(
lambda: self.Motor_move_upwards())
# self.ObjMotor_upwards.setShortcut('w')
self.ObjMotor_down = QPushButton("↓")
self.ObjMotor_down.setStyleSheet(
"QPushButton {color:white;background-color: teal; border-style: outset;border-radius: 10px;border-width: 2px;font: bold 14px;padding: 6px}"
"QPushButton:pressed {color:red;background-color: white; border-style: outset;border-radius: 10px;border-width: 2px;font: bold 14px;padding: 6px}"
)
self.ObjMotorcontrolLayout.addWidget(self.ObjMotor_down, 3, 2)
self.ObjMotor_down.clicked.connect(lambda: self.Motor_move_downwards())
# self.stage_down.setShortcut('s')
self.ObjMotor_target = QDoubleSpinBox(self)
self.ObjMotor_target.setMinimum(-10000)
self.ObjMotor_target.setMaximum(10000)
self.ObjMotor_target.setDecimals(6)
# self.ObjMotor_target.setValue(3.45)
self.ObjMotor_target.setSingleStep(0.001)
self.ObjMotorcontrolLayout.addWidget(self.ObjMotor_target, 1, 1)
self.ObjMotorcontrolLayout.addWidget(QLabel("Target:"), 1, 0)
self.ObjMotor_current_pos_Label = QLabel("Current position: ")
self.ObjMotorcontrolLayout.addWidget(self.ObjMotor_current_pos_Label,
2, 0)
self.ObjMotor_goto = QPushButton("Move")
self.ObjMotor_goto.setStyleSheet(
"QPushButton {color:white;background-color: blue; border-style: outset;border-radius: 10px;border-width: 2px;font: bold 14px;padding: 6px}"
"QPushButton:pressed {color:red;background-color: white; border-style: outset;border-radius: 10px;border-width: 2px;font: bold 14px;padding: 6px}"
)
self.ObjMotorcontrolLayout.addWidget(self.ObjMotor_goto, 1, 2)
self.ObjMotor_goto.clicked.connect(self.MoveMotor)
self.ObjMotor_step = QDoubleSpinBox(self)
self.ObjMotor_step.setMinimum(-10000)
self.ObjMotor_step.setMaximum(10000)
self.ObjMotor_step.setDecimals(6)
self.ObjMotor_step.setValue(0.001)
self.ObjMotor_step.setSingleStep(0.001)
self.ObjMotorcontrolLayout.addWidget(self.ObjMotor_step, 3, 1)
self.ObjMotorcontrolLayout.addWidget(QLabel("Step: "), 3, 0)
ObjMotorcontrolContainer.setLayout(self.ObjMotorcontrolLayout)
ObjMotorcontrolContainer.setMaximumHeight(300)
self.layout.addWidget(ObjMotorcontrolContainer, 4, 0)
#**************************************************************************************************************************************
#--------------------------------------------------------------------------------------------------------------------------------------
#-----------------------------------------------------------GUI for camera button------------------------------------------------------
#--------------------------------------------------------------------------------------------------------------------------------------
#**************************************************************************************************************************************
self.open_cam = QPushButton('Open Camera')
self.open_cam.clicked.connect(self.open_camera)
self.layout.addWidget(self.open_cam, 5, 0)
self.console_text_edit = QTextEdit()
self.console_text_edit.setFontItalic(True)
self.console_text_edit.setPlaceholderText('Notice board from console.')
self.console_text_edit.setMaximumHeight(200)
self.layout.addWidget(self.console_text_edit, 6, 0)
#**************************************************************************************************************************************
#self.setLayout(pmtmaster)
self.layout.addWidget(self.tabs, 0, 1, 8, 4)
self.setLayout(self.layout)
'''