def main(input_files, out_csv_file,
threads=5, extract_regions=True, known_names=None):
seq_iter = fasta_reader(FileInput(input_files))
found_names = set()
need_header = True
if os.path.exists(out_csv_file):
need_header = False
with open(out_csv_file) as handle:
for row in csv.DictReader(handle, delimiter='\t'):
found_names.add(row['Name'])
csv_handle = open(out_csv_file, 'a')
fields = ['Name', 'RegionName', 'QueryNucStart', 'QueryNucStop', 'QueryNuc',
'RegionNucStart', 'RegionNucStop', 'RegionAAStart', 'RegionAAStop', 'QueryAA']
csv_writer = csv.DictWriter(csv_handle, fields, delimiter='\t')
if need_header:
csv_writer.writeheader()
wanted_seqs = ((name, seq) for name, seq in seq_iter if name not in found_names)
result_iter = process_seqs(wanted_seqs,
threads=threads,
extract_regions=extract_regions,
known_names=known_names)
for chunk in yield_chunks(result_iter, 1000):
print chunk[0]
csv_writer.writerows(chunk)
def large_cluster(iseqs, cluster_size=1000):
running_align = 'running_profile.fasta'
ifile = 'adding_fasta.fasta'
#do base alignment
chunk_seqs = take(cluster_size, iseqs)
muscle_align(chunk_seqs, running_align)
count = len(chunk_seqs)
print 'Seqs Processed:', count
chunk_seqs = take(cluster_size, iseqs)
while chunk_seqs:
count += len(chunk_seqs)
muscle_align(chunk_seqs, ifile)
shutil.move(running_align, running_align + '.tmp')
muscle_join(running_align + '.tmp', ifile, running_align)
chunk_seqs = take(cluster_size, iseqs)
print 'Seqs Processed:', count
shutil.move(running_align, running_align + '.tmp')
print 'refining!'
cmd = 'muscle -in %s -out %s -refine' % (running_align + '.tmp', running_align)
check_call(shlex.split(cmd))
with open(running_align) as handle:
return list(fasta_reader(handle))
def filter_seq(handle, trans):
for name, seq in fasta_reader(handle):
tseq = ''.join(l for l in seq if l.isalpha())
l = len(tseq)
if (l > 100) and (l < 120):
if trans:
rseq = Seq(tseq, generic_dna).translate()
yield name, ''.join(l for l in rseq.tostring() if l.isalpha())
else:
yield name, tseq
def trans_seq(handle, wanted_seqs, trans=True):
for name, seq in fasta_reader(handle):
if name not in wanted_seqs:
continue
tseq = ''.join(l for l in seq if l.isalpha())
if trans:
rseq = Seq(tseq, generic_dna).translate()
yield name, ''.join(l for l in rseq.tostring() if l.isalpha())
else:
yield name, tseq
def get_from_fasta_handle(handle, letters_only=True):
names = []
seqs = []
for name, seq in fasta_reader(handle):
names.append(name)
if letters_only:
seqs.append(''.join(l for l in seq if l.isalpha()))
else:
seqs.append(seq)
return names, SeqTransformer().fit_transform(seqs)
def test_known_mappings():
with open('TestData/LocatorRes.tsv') as handle:
cor_res = list(csv.DictReader(handle, delimiter='\t'))
with open('TestData/testSeqs.fasta') as handle:
test_seqs = list(fasta_reader(handle))
for row, crow in zip(HIVTransTool.process_seqs(test_seqs, extract_regions=True), cor_res):
for f in crow.keys():
if row[f] is None:
row[f] = ''
yield eq_, str(row[f]), crow[f], f
from GeneralSeqTools import fasta_reader
import csv
# <codecell>
from HIVTransTool import map_seqs_to_ref, process_seqs
import csv
ref_path = 'HIVDBFiles/HXB2Sequence.fasta'
cor_res = {}
with open('TestData/test_mapping.csv') as handle:
for row in csv.DictReader(handle, delimiter='\t'):
cor_res[row['SeqName']] = (int(row['GenomeStart']), int(row['GenomeStop']))
with open('TestData/testSeqs.fasta') as handle:
input_seqs = list(fasta_reader(handle))
#with open('/home/will/HIVRate/hiv-db.fasta') as handle:
# seqs = list(fasta_reader(handle))
# <codecell>
from itertools import product
list(product('abcdefg', range(5)))
# <codecell>
fields = ['Name','RegionName', 'QueryNucStart','QueryNucStop','QueryNuc',
'RegionNucStart','RegionNucStop','RegionAAStart', 'RegionAAStop', 'QueryAA']
os.chdir('/home/will/SadiVariation/')
sys.path.append('/home/will/PySeqUtils/')
# <codecell>
from GeneralSeqTools import fasta_reader, fasta_writer, WebPSSM_V3_series
import glob
# <codecell>
files = [('x4_seqs.fasta.old', 'x4_seqs.fasta'),
('r5_seqs.fasta.old', 'r5_seqs.fasta')]
for ifile, ofile in files:
with open(ifile) as handle:
with open(ofile, 'w') as ohandle:
for name, seq in fasta_reader(handle):
fasta_writer(ohandle, [(name, seq[1:-1])])
# <codecell>
subtype_files = glob.glob('/home/will/WLAHDB_data/SubtypeGuess/*.gb')
subtypes = []
for f in subtype_files:
gb = f.rsplit(os.sep, 1)[-1].split('.')[0]
with open(f) as handle:
subtype = handle.next().strip()
if subtype != 'Unk':
subtypes.append((int(gb), subtype))
subtype_df = pd.DataFrame(subtypes, columns = ['GI', 'Subtype'])
subtype_ser = subtype_df.groupby('GI')['Subtype'].first()
import os, os.path
import sys
import numpy as np
sys.path.append('/home/will/HIVReportGen/AnalysisCode/')
sys.path.append('/home/will/PySeqUtils/')
os.chdir('/home/will/HIVVariation/')
from GeneralSeqTools import call_muscle
# <codecell>
from GeneralSeqTools import fasta_reader
seq_data = []
with open('PBMC_analyzed.clean.fasta') as handle:
for name, seq in fasta_reader(handle):
try:
pid, vn = name.split('-')[0:2]
except ValueError:
print name
raise ValueError
seq_data.append((pid, vn, seq))
seq_df = DataFrame(seq_data, columns=['Patient ID', 'VisitNum', 'Seq'])
# <codecell>
wanted_seq_cols = [340, 381] #1-based!!
hxb2_ltr = """TGGAAGGGCTAATTTACTCCCAAAAAAGACAAGATATCCTTGATCTGTGGGTC
TACCACACACAAGGCTACTTCCCTGATTGGCAGAACTACACACCAGGGCCAGG
GATCAGATATCCACTGACCTTTGGATGGTGCTTCAAGCTAGTACCAGTTGAGC
#
ax.set_title(col + ' pval:%f' % pval)
ax.set_ylim([0, ax.get_ylim()[1]])
plt.tight_layout()
plt.savefig(base_path + 'corrected_cyto_data.png')
# <codecell>
import glob
ltr_files = sorted(glob.glob('/home/will/HIVReportGen/Data/PatientFasta/*LTR.fasta'))
ltr_seqs = []
for f in ltr_files:
with open(f) as handle:
ltr_seqs += list(fasta_reader(handle))
print len(ltr_seqs)
# <codecell>
conb_ltr = """TGGAAGGGCTAATTCACTCCCAACGAAGACAAGATATCCTTGATCTGTGGATCTACCACACACAA
GGCTACTTCCCTGATTAGCAGAACTACACACCAGGGCCAGGGATCAGATATCCACTGACCTTTGGATGGTGCTACAAGC
TAGTACCAGTTGAGCCAGAGAAGTTAGAAGAAGCCAACAAAGGAGAGAACACCAGCTTGTTACACCCTGTGAGCCTGCA
TGGAATGGATGACCCGGAGAGAGAAGTGTTAGAGTGGAGGTTTGACAGCCGCCTAGCATTTCATCACATGGCCCGAGAG
CTGCATCCGGAGTACTTCAAGAACTGCTGACATCGAGCTTGCTACAAGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC
GTGGCCTGGGCGGGACTGGGGAGTGGCGAGCCCTCAGATCCTGCATATAAGCAGCTGCTTTTTGCCTGTACTGGGTCTC
TCTGGTTAGACCAGATCTGAGCCTGGGAGCTCTCTGGCTAACTAGGGAACCCACTGCTTAAGCCTCAATAAAGCTTGCC
TTGAGTGCTTCAAGTAGTGTGTGCCCGTCTGTTGTGTGACTCTGGTAACTAGAGATCCCTCAGACCCTTTTAGTCAGTG
TGGAAAATCTCT""".replace('\n', '')
ltr_align = list(seq_align_to_ref(ltr_seqs, conb_ltr, max_workers = 20))
def yield_lets(infile):
with open(infile) as handle:
for name, seq in fasta_reader(handle):
for l in imap(lambda x: x.upper(), seq):
if l != '-':
yield l.upper()
def filter_seq(handle, trans):
for name, seq in fasta_reader(handle):
tseq = ''.join(l for l in seq if l.isalpha())
l = len(tseq)
if (l == 105):
if trans:
rseq = Seq(tseq, generic_dna).translate()
yield name, rseq.tostring()
else:
yield name, tseq
with open('V3filter.nt.fasta.raln') as handle:
seq_list = list(fasta_reader(handle))
with open('V3filter.aa.fasta.raln') as handle:
aa_seq_list = list(fasta_reader(handle))
# <codecell>
import numpy as np
from sklearn.base import BaseEstimator, TransformerMixin, ClassifierMixin
from itertools import product
from scipy.sparse import csr_matrix, eye
class BioTransformer(BaseEstimator, TransformerMixin):
sys.path.append('/home/will/HIVReportGen/AnalysisCode/')
sys.path.append('/home/will/PySeqUtils/')
# <codecell>
from GeneralSeqTools import fasta_reader,
# <codecell>
tat_ex1_pos = (5830, 6044) #0 based
pos_data = read_csv('simple_results.txt', sep = '\t')
with open('Tat1-AB1_passed-cleaned.fasta') as handle:
seq_data = list(fasta_reader(handle))
# <codecell>
from Bio.Seq import Seq
from Bio.Alphabet import generic_dna, generic_protein
def translate_to_tat(inseq, start_pos, end_pos, rep = 0):
if rep == 2:
print 'ESCAPED!!!'
return
nstart = tat_ex1_pos[0] - start_pos
nend = tat_ex1_pos[1] - end_pos
def load_fasta_to_db(fasta_files, source, is_nuc=True, RegionName='Genome'):
seq_iterable = fasta_reader(FileInput(fasta_files))
load_raw_seqs_to_db(seq_iterable, source, is_nuc=is_nuc, RegionName=RegionName)
print num, len(block)
# <codecell>
blast_all_v_all(sA, sB)
# <codecell>
with open('/home/will/tmpstuf/haptest/DrexelMed.A0107.R02.fa') as handle:
sA = list(fasta_reader(handle))
with open('/home/will/tmpstuf/haptest/DrexelMed.A0107.fa') as handle:
sB = list(fasta_reader(handle))
# <codecell>
sA[:5]
# <codecell>
import sys
sys.path.append('/home/will/PySeqUtils/')
from GeneralSeqTools import fasta_reader, fasta_writer
import os
os.chdir('/home/will/PySeqUtils/TransToolStuff/')
# <codecell>
from itertools import islice
start = 806
stop = -1
path = 'HIV1_ALL_2012_env_PRO.fasta'
outpath = 'HIV1_ALL_2012_gp41_PRO.fasta'
with open(path) as handle:
for name, seq in islice(fasta_reader(handle), 20):
tseq = seq[start:stop]
print tseq[:5], tseq[-5:]
# <codecell>
seqs = []
with open(path) as handle:
for name, seq in fasta_reader(handle):
seqs.append((name, seq[start:stop]))
with open(outpath, 'w') as handle:
fasta_writer(handle, seqs)
# <codecell>
from Bio import Entrez
