def OnTagPoints(self, event):
from PYME.PSFEst import extractImages
chnum = self.chChannel.GetSelection()
rsx, rsy, rsz = [int(s) for s in self.tPSFROI.GetValue().split(',')]
for xp, yp, zp in self.view.points:
if ((xp > rsx) and (xp < (self.image.data.shape[0] - rsx)) and
(yp > rsy) and (yp < (self.image.data.shape[1] - rsy))):
dx, dy, dz = extractImages.getIntCenter(self.image.data[(xp-rsx):(xp+rsx + 1),(yp-rsy):(yp+rsy+1), :, chnum])
self.PSFLocs.append((xp + dx, yp + dy, dz))
#self.view.psfROIs = self.PSFLocs
self.view.Refresh()
def OnTagPoints(self, event):
from PYME.PSFEst import extractImages
chnum = self.chChannel.GetSelection()
rsx, rsy, rsz = [int(s) for s in self.tPSFROI.GetValue().split(',')]
for xp, yp, zp in self.view.points:
if ((xp > rsx) and (xp < (self.image.data.shape[0] - rsx))
and (yp > rsy) and (yp <
(self.image.data.shape[1] - rsy))):
dx, dy, dz = extractImages.getIntCenter(
self.image.data[(xp - rsx):(xp + rsx + 1),
(yp - rsy):(yp + rsy + 1), :, chnum])
self.PSFLocs.append((xp + dx, yp + dy, dz))
#self.view.psfROIs = self.PSFLocs
self.view.Refresh()
def OnTagPSF(self, event):
from PYME.PSFEst import extractImages
#if we already have a location there, un-tag it
for i, p in enumerate(self.PSFLocs):
if ((numpy.array(p[:2]) - numpy.array((self.do.xp, self.do.yp)))**2).sum() < 100:
self.PSFLocs.pop(i)
#self.view.psfROIs = self.PSFLocs
self.view.Refresh()
return
#if we have a muilt-colour stack,
chnum = self.chChannel.GetSelection()
rsx, rsy, rsz = [int(s) for s in self.tPSFROI.GetValue().split(',')]
dx, dy, dz = extractImages.getIntCenter(self.image.data[(self.do.xp-rsx):(self.do.xp+rsx + 1),(self.do.yp-rsy):(self.do.yp+rsy+1), :, chnum])
self.PSFLocs.append((self.do.xp + dx, self.do.yp + dy, dz))
self.view.psfROIs = self.PSFLocs
self.view.Refresh()
def OnTagPSF(self, event):
from PYME.PSFEst import extractImages
#if we already have a location there, un-tag it
for i, p in enumerate(self.PSFLocs):
if ((numpy.array(p[:2]) - numpy.array(
(self.do.xp, self.do.yp)))**2).sum() < 100:
self.PSFLocs.pop(i)
#self.view.psfROIs = self.PSFLocs
self.view.Refresh()
return
#if we have a muilt-colour stack,
chnum = self.chChannel.GetSelection()
rsx, rsy, rsz = [int(s) for s in self.tPSFROI.GetValue().split(',')]
dx, dy, dz = extractImages.getIntCenter(
self.image.data[(self.do.xp - rsx):(self.do.xp + rsx + 1),
(self.do.yp - rsy):(self.do.yp + rsy + 1), :,
chnum])
self.PSFLocs.append((self.do.xp + dx, self.do.yp + dy, dz))
self.view.psfROIs = self.PSFLocs
self.view.Refresh()