def __next__(self):
try:
line = self._header
del self._header
except AttributeError:
line = self.handle.readline()
if not line:
# Empty file - just give up.
raise StopIteration
if not line.strip() == "# STOCKHOLM 1.0":
raise ValueError("Did not find STOCKHOLM header")
# Note: If this file follows the PFAM conventions, there should be
# a line containing the number of sequences, e.g. "#=GF SQ 67"
# We do not check for this - perhaps we should, and verify that
# if present it agrees with our parsing.
seqs = {}
ids = []
gs = {}
gr = {}
gf = {}
passed_end_alignment = False
while True:
line = self.handle.readline()
if not line:
break # end of file
line = line.strip() # remove trailing \n
if line == "# STOCKHOLM 1.0":
self._header = line
break
elif line == "//":
# The "//" line indicates the end of the alignment.
# There may still be more meta-data
passed_end_alignment = True
elif line == "":
# blank line, ignore
pass
elif line[0] != "#":
# Sequence
# Format: "<seqname> <sequence>"
assert not passed_end_alignment
parts = [x.strip() for x in line.split(" ", 1)]
if len(parts) != 2:
# This might be someone attempting to store a zero length sequence?
raise ValueError("Could not split line into identifier " + "and sequence:\n" + line)
id, seq = parts
if id not in ids:
ids.append(id)
seqs.setdefault(id, "")
seqs[id] += seq.replace(".", "-")
elif len(line) >= 5:
# Comment line or meta-data
if line[:5] == "#=GF ":
# Generic per-File annotation, free text
# Format: #=GF <feature> <free text>
feature, text = line[5:].strip().split(None, 1)
# Each feature key could be used more than once,
# so store the entries as a list of strings.
if feature not in gf:
gf[feature] = [text]
else:
gf[feature].append(text)
elif line[:5] == "#=GC ":
# Generic per-Column annotation, exactly 1 char per column
# Format: "#=GC <feature> <exactly 1 char per column>"
pass
elif line[:5] == "#=GS ":
# Generic per-Sequence annotation, free text
# Format: "#=GS <seqname> <feature> <free text>"
id, feature, text = line[5:].strip().split(None, 2)
# if id not in ids:
# ids.append(id)
if id not in gs:
gs[id] = {}
if feature not in gs[id]:
gs[id][feature] = [text]
else:
gs[id][feature].append(text)
elif line[:5] == "#=GR ":
# Generic per-Sequence AND per-Column markup
# Format: "#=GR <seqname> <feature> <exactly 1 char per column>"
id, feature, text = line[5:].strip().split(None, 2)
# if id not in ids:
# ids.append(id)
if id not in gr:
gr[id] = {}
if feature not in gr[id]:
gr[id][feature] = ""
gr[id][feature] += text.strip() # append to any previous entry
# TODO - Should we check the length matches the alignment length?
# For iterlaced sequences the GR data can be split over
# multiple lines
# Next line...
assert len(seqs) <= len(ids)
# assert len(gs) <= len(ids)
# assert len(gr) <= len(ids)
self.ids = ids
self.sequences = seqs
self.seq_annotation = gs
self.seq_col_annotation = gr
if ids and seqs:
if self.records_per_alignment is not None and self.records_per_alignment != len(ids):
raise ValueError(
"Found %i records in this alignment, told to expect %i" % (len(ids), self.records_per_alignment)
)
alignment_length = len(list(seqs.values())[0])
records = [] # Alignment obj will put them all in a list anyway
for id in ids:
seq = seqs[id]
if alignment_length != len(seq):
raise ValueError("Sequences have different lengths, or repeated identifier")
name, start, end = self._identifier_split(id)
record = SeqRecord(
Seq(seq, self.alphabet), id=id, name=name, description=id, annotations={"accession": name}
)
# Accession will be overridden by _populate_meta_data if an explicit
# accession is provided:
record.annotations["accession"] = name
if start is not None:
record.annotations["start"] = start
if end is not None:
record.annotations["end"] = end
self._populate_meta_data(id, record)
records.append(record)
alignment = MultipleSeqAlignment(records, self.alphabet)
# TODO - Introduce an annotated alignment class?
# For now, store the annotation a new private property:
alignment._annotations = gr
return alignment
else:
raise StopIteration
def __next__(self):
try:
line = self._header
del self._header
except AttributeError:
line = self.handle.readline()
if not line:
#Empty file - just give up.
raise StopIteration
if not line.strip() == '# STOCKHOLM 1.0':
raise ValueError("Did not find STOCKHOLM header")
# Note: If this file follows the PFAM conventions, there should be
# a line containing the number of sequences, e.g. "#=GF SQ 67"
# We do not check for this - perhaps we should, and verify that
# if present it agrees with our parsing.
seqs = {}
ids = []
gs = {}
gr = {}
gf = {}
passed_end_alignment = False
while True:
line = self.handle.readline()
if not line:
break # end of file
line = line.strip() # remove trailing \n
if line == '# STOCKHOLM 1.0':
self._header = line
break
elif line == "//":
#The "//" line indicates the end of the alignment.
#There may still be more meta-data
passed_end_alignment = True
elif line == "":
#blank line, ignore
pass
elif line[0] != "#":
#Sequence
#Format: "<seqname> <sequence>"
assert not passed_end_alignment
parts = [x.strip() for x in line.split(" ", 1)]
if len(parts) != 2:
#This might be someone attempting to store a zero length sequence?
raise ValueError("Could not split line into identifier "
+ "and sequence:\n" + line)
id, seq = parts
if id not in ids:
ids.append(id)
seqs.setdefault(id, '')
seqs[id] += seq.replace(".", "-")
elif len(line) >= 5:
#Comment line or meta-data
if line[:5] == "#=GF ":
#Generic per-File annotation, free text
#Format: #=GF <feature> <free text>
feature, text = line[5:].strip().split(None, 1)
#Each feature key could be used more than once,
#so store the entries as a list of strings.
if feature not in gf:
gf[feature] = [text]
else:
gf[feature].append(text)
elif line[:5] == '#=GC ':
#Generic per-Column annotation, exactly 1 char per column
#Format: "#=GC <feature> <exactly 1 char per column>"
pass
elif line[:5] == '#=GS ':
#Generic per-Sequence annotation, free text
#Format: "#=GS <seqname> <feature> <free text>"
id, feature, text = line[5:].strip().split(None, 2)
#if id not in ids:
# ids.append(id)
if id not in gs:
gs[id] = {}
if feature not in gs[id]:
gs[id][feature] = [text]
else:
gs[id][feature].append(text)
elif line[:5] == "#=GR ":
#Generic per-Sequence AND per-Column markup
#Format: "#=GR <seqname> <feature> <exactly 1 char per column>"
id, feature, text = line[5:].strip().split(None, 2)
#if id not in ids:
# ids.append(id)
if id not in gr:
gr[id] = {}
if feature not in gr[id]:
gr[id][feature] = ""
gr[id][feature] += text.strip() # append to any previous entry
#TODO - Should we check the length matches the alignment length?
# For iterlaced sequences the GR data can be split over
# multiple lines
#Next line...
assert len(seqs) <= len(ids)
#assert len(gs) <= len(ids)
#assert len(gr) <= len(ids)
self.ids = ids
self.sequences = seqs
self.seq_annotation = gs
self.seq_col_annotation = gr
if ids and seqs:
if self.records_per_alignment is not None \
and self.records_per_alignment != len(ids):
raise ValueError("Found %i records in this alignment, told to expect %i"
% (len(ids), self.records_per_alignment))
alignment_length = len(list(seqs.values())[0])
records = [] # Alignment obj will put them all in a list anyway
for id in ids:
seq = seqs[id]
if alignment_length != len(seq):
raise ValueError("Sequences have different lengths, or repeated identifier")
name, start, end = self._identifier_split(id)
record = SeqRecord(Seq(seq, self.alphabet),
id=id, name=name, description=id,
annotations={"accession": name})
#Accession will be overridden by _populate_meta_data if an explicit
#accession is provided:
record.annotations["accession"] = name
if start is not None:
record.annotations["start"] = start
if end is not None:
record.annotations["end"] = end
self._populate_meta_data(id, record)
records.append(record)
alignment = MultipleSeqAlignment(records, self.alphabet)
#TODO - Introduce an annotated alignment class?
#For now, store the annotation a new private property:
alignment._annotations = gr
return alignment
else:
raise StopIteration
def build_hsp():
if not query_tags and not match_tags:
raise ValueError("No data for query %r, match %r"
% (query_id, match_id))
assert query_tags, query_tags
assert match_tags, match_tags
evalue = align_tags.get("fa_expect", None)
q = "?" # Just for printing len(q) in debug below
m = "?" # Just for printing len(m) in debug below
tool = global_tags.get("tool", "").upper()
try:
q = _extract_alignment_region(query_seq, query_tags)
if tool in ["TFASTX"] and len(match_seq) == len(q):
m = match_seq
#Quick hack until I can work out how -, * and / characters
#and the apparent mix of aa and bp coordinates works.
else:
m = _extract_alignment_region(match_seq, match_tags)
assert len(q) == len(m)
except AssertionError as err:
print("Darn... amino acids vs nucleotide coordinates?")
print(tool)
print(query_seq)
print(query_tags)
print("%s %i" % (q, len(q)))
print(match_seq)
print(match_tags)
print("%s %i" % (m, len(m)))
print(handle.name)
raise err
assert alphabet is not None
alignment = MultipleSeqAlignment([], alphabet)
#TODO - Introduce an annotated alignment class?
#For now, store the annotation a new private property:
alignment._annotations = {}
#Want to record both the query header tags, and the alignment tags.
for key, value in header_tags.items():
alignment._annotations[key] = value
for key, value in align_tags.items():
alignment._annotations[key] = value
#Query
#=====
record = SeqRecord(Seq(q, alphabet),
id=query_id,
name="query",
description=query_descr,
annotations={"original_length": int(query_tags["sq_len"])})
#TODO - handle start/end coordinates properly. Short term hack for now:
record._al_start = int(query_tags["al_start"])
record._al_stop = int(query_tags["al_stop"])
alignment.append(record)
#TODO - What if a specific alphabet has been requested?
#TODO - Use an IUPAC alphabet?
#TODO - Can FASTA output RNA?
if alphabet == single_letter_alphabet and "sq_type" in query_tags:
if query_tags["sq_type"] == "D":
record.seq.alphabet = generic_dna
elif query_tags["sq_type"] == "p":
record.seq.alphabet = generic_protein
if "-" in q:
if not hasattr(record.seq.alphabet, "gap_char"):
record.seq.alphabet = Gapped(record.seq.alphabet, "-")
#Match
#=====
record = SeqRecord(Seq(m, alphabet),
id=match_id,
name="match",
description=match_descr,
annotations={"original_length": int(match_tags["sq_len"])})
#TODO - handle start/end coordinates properly. Short term hack for now:
record._al_start = int(match_tags["al_start"])
record._al_stop = int(match_tags["al_stop"])
alignment.append(record)
#This is still a very crude way of dealing with the alphabet:
if alphabet == single_letter_alphabet and "sq_type" in match_tags:
if match_tags["sq_type"] == "D":
record.seq.alphabet = generic_dna
elif match_tags["sq_type"] == "p":
record.seq.alphabet = generic_protein
if "-" in m:
if not hasattr(record.seq.alphabet, "gap_char"):
record.seq.alphabet = Gapped(record.seq.alphabet, "-")
return alignment
def build_hsp():
if not query_tags and not match_tags:
raise ValueError("No data for query %r, match %r" %
(query_id, match_id))
assert query_tags, query_tags
assert match_tags, match_tags
evalue = align_tags.get("fa_expect", None)
q = "?" # Just for printing len(q) in debug below
m = "?" # Just for printing len(m) in debug below
tool = global_tags.get("tool", "").upper()
try:
q = _extract_alignment_region(query_seq, query_tags)
if tool in ["TFASTX"] and len(match_seq) == len(q):
m = match_seq
#Quick hack until I can work out how -, * and / characters
#and the apparent mix of aa and bp coordinates works.
else:
m = _extract_alignment_region(match_seq, match_tags)
assert len(q) == len(m)
except AssertionError as err:
print("Darn... amino acids vs nucleotide coordinates?")
print(tool)
print(query_seq)
print(query_tags)
print("%s %i" % (q, len(q)))
print(match_seq)
print(match_tags)
print("%s %i" % (m, len(m)))
print(handle.name)
raise err
assert alphabet is not None
alignment = MultipleSeqAlignment([], alphabet)
#TODO - Introduce an annotated alignment class?
#For now, store the annotation a new private property:
alignment._annotations = {}
#Want to record both the query header tags, and the alignment tags.
for key, value in header_tags.items():
alignment._annotations[key] = value
for key, value in align_tags.items():
alignment._annotations[key] = value
#Query
#=====
record = SeqRecord(
Seq(q, alphabet),
id=query_id,
name="query",
description=query_descr,
annotations={"original_length": int(query_tags["sq_len"])})
#TODO - handle start/end coordinates properly. Short term hack for now:
record._al_start = int(query_tags["al_start"])
record._al_stop = int(query_tags["al_stop"])
alignment.append(record)
#TODO - What if a specific alphabet has been requested?
#TODO - Use an IUPAC alphabet?
#TODO - Can FASTA output RNA?
if alphabet == single_letter_alphabet and "sq_type" in query_tags:
if query_tags["sq_type"] == "D":
record.seq.alphabet = generic_dna
elif query_tags["sq_type"] == "p":
record.seq.alphabet = generic_protein
if "-" in q:
if not hasattr(record.seq.alphabet, "gap_char"):
record.seq.alphabet = Gapped(record.seq.alphabet, "-")
#Match
#=====
record = SeqRecord(
Seq(m, alphabet),
id=match_id,
name="match",
description=match_descr,
annotations={"original_length": int(match_tags["sq_len"])})
#TODO - handle start/end coordinates properly. Short term hack for now:
record._al_start = int(match_tags["al_start"])
record._al_stop = int(match_tags["al_stop"])
alignment.append(record)
#This is still a very crude way of dealing with the alphabet:
if alphabet == single_letter_alphabet and "sq_type" in match_tags:
if match_tags["sq_type"] == "D":
record.seq.alphabet = generic_dna
elif match_tags["sq_type"] == "p":
record.seq.alphabet = generic_protein
if "-" in m:
if not hasattr(record.seq.alphabet, "gap_char"):
record.seq.alphabet = Gapped(record.seq.alphabet, "-")
return alignment
