def queueWorker(self):
debugModule = False
quitSignal = False
oldFilename = ''
oldArchive = ''
oldSlide = ''
oldThres = -1
while not quitSignal:
job = SlideRunnerPlugin.pluginJob(self.inQueue.get())
print(job)
if (job.jobDescription ==
SlideRunnerPlugin.JobDescription.QUIT_PLUGIN_THREAD):
# signal to exit this thread
quitSignal = True
continue
if (job.configuration['file'] == oldArchive) and (job.slideFilename
== oldSlide):
continue
if not (os.path.exists(job.configuration['file'])):
continue
self.sendAnnotationLabelUpdate()
oldArchive = job.configuration['file']
oldSlide = job.slideFilename
self.secondaryDB.open(oldArchive)
self.annos = list()
self.annotationLabels = dict()
for key, (label, annoId,
col) in enumerate(self.secondaryDB.getAllClasses()):
self.annotationLabels[
annoId] = SlideRunnerPlugin.PluginAnnotationLabel(
0, '%s' % label, [*hex_to_rgb(col), 0])
pname, fname = os.path.split(job.slideFilename)
self.slideUID = self.secondaryDB.findSlideWithFilename(
fname, pname)
self.secondaryDB.loadIntoMemory(self.slideUID)
self.annos = list()
for annoId in self.secondaryDB.annotations.keys():
anno = self.secondaryDB.annotations[annoId]
anno.pluginAnnotationLabel = self.annotationLabels[
anno.agreedClass]
anno.clickable = job.configuration['mode'] == 0
self.annos.append(anno)
self.sendAnnotationLabelUpdate()
self.updateAnnotations()
self.setProgressBar(-1)
self.setMessage('found %d annotations.' % len(self.annos))
def queueWorker(self):
quitSignal = False
while not quitSignal:
job = SlideRunnerPlugin.pluginJob(self.inQueue.get())
self.total_number = int(job.configuration[0])
if (job.jobDescription ==
SlideRunnerPlugin.JobDescription.QUIT_PLUGIN_THREAD):
# signal to exit this thread
quitSignal = True
continue
database = job.openedDatabase
annotations_count = len(database.annotations)
if annotations_count != self.last_count:
self.last_count = annotations_count
if self.total_number - annotations_count == 0:
self.setMessage('Done. Thanks for your help :)')
self.showMessageBox('Done. Thanks for your help :)')
elif self.total_number - annotations_count < 0:
self.setMessage(
'You are ambitious, thats gread, thank you')
else:
self.setMessage(
'{0} anotations to go'.format(self.total_number -
annotations_count))
def queueWorker(self):
quitSignal = False
while not quitSignal:
job = SlideRunnerPlugin.pluginJob(self.inQueue.get())
image = job.currentImage
if (job.jobDescription ==
SlideRunnerPlugin.JobDescription.QUIT_PLUGIN_THREAD):
# signal to exit this thread
quitSignal = True
continue
print('OTSU plugin: received 1 image from queue')
self.setProgressBar(0)
rgb = np.copy(image[:, :, 0:3])
gray = cv2.cvtColor(rgb, cv2.COLOR_RGB2GRAY)
# OTSU thresholding
ret, thresh = cv2.threshold(
gray, 0, 255, cv2.THRESH_BINARY_INV + cv2.THRESH_OTSU)
self.returnImage(np.float32(thresh / 255.0), job.procId)
self.setMessage('OTSU calculation done.')
print('OTSU plugin: done')
self.setProgressBar(-1)
def processWholeSlide(self, job: SlideRunnerPlugin.pluginJob):
filename = job.slideFilename
self.slide = openslide.open_slide(filename)
# 1 HPF = 0.237 mm^2
A = 0.237 # mm^2
W_hpf_microns = np.sqrt(A * 4 / 3) * 1000 # in microns
H_hpf_microns = np.sqrt(A * 3 / 4) * 1000 # in microns
micronsPerPixel = self.slide.properties[openslide.PROPERTY_NAME_MPP_X]
W_hpf = int(W_hpf_microns / float(micronsPerPixel))
H_hpf = int(H_hpf_microns / float(micronsPerPixel))
center = (int((job.coordinates[0] + 0.5 * job.coordinates[2])),
int((job.coordinates[1] + 0.5 * job.coordinates[3])))
self.annos = list()
myanno = SlideRunnerPlugin.rectangularAnnotation(
center[0] - W_hpf / 2, center[1] - H_hpf / 2,
center[0] + W_hpf / 2, center[1] + H_hpf / 2, 'High-Power Field')
self.annos.append(myanno)
self.updateAnnotations()
def __init__(self, statusQueue: Queue):
self.statusQueue = statusQueue
self.annotationLabels = {
'Detection':
SlideRunnerPlugin.PluginAnnotationLabel(0, 'Detection',
[0, 180, 0, 255]),
}
self.p = Thread(target=self.queueWorker, daemon=True)
self.p.start()
pass
def queueWorker(self):
quitSignal = False
while not quitSignal:
job = SlideRunnerPlugin.pluginJob(self.inQueue.get())
image = job.currentImage
if (job.jobDescription ==
SlideRunnerPlugin.JobDescription.QUIT_PLUGIN_THREAD):
# signal to exit this thread
quitSignal = True
continue
print('Macenko norm plugin: received 1 image from queue')
self.setProgressBar(0)
print(job)
if (job.annotations is not None) and len(job.annotations) > 0:
if (job.annotations[0].annotationType ==
annotations.AnnotationType.AREA):
print('Found an area annotation - great!')
minC = job.annotations[0].minCoordinates()
maxC = job.annotations[0].maxCoordinates()
scaleX = (job.coordinates[2]) / job.currentImage.shape[1]
scaleY = (job.coordinates[3]) / job.currentImage.shape[0]
minC = np.array(
(max(0, int((minC.x - job.coordinates[0]) / scaleX)),
max(0, int((minC.y - job.coordinates[1]) / scaleY))))
maxC = np.array(
(min(job.currentImage.shape[1],
int((maxC.x - job.coordinates[0]) / scaleX)),
min(job.currentImage.shape[0],
int((maxC.y - job.coordinates[1]) / scaleY))))
rgb = np.copy(image[:, :, 0:3])
rgb = normalize(rgb,
np.reshape(
rgb[minC[1]:maxC[1],
minC[0]:maxC[0], :], (-1, 3)),
job=job)
else:
rgb = np.copy(image[:, :, 0:3])
rgb = normalize(rgb, job=job)
print('Stats: ', np.max(np.float32(rgb)), np.min(np.float32(rgb)),
np.mean(np.float32(rgb)),
np.float32(rgb).shape)
self.returnImage(np.float32(rgb), job.procId)
self.setMessage('Macenko normalization: done.')
self.setProgressBar(-1)
class Plugin(SlideRunnerPlugin.SlideRunnerPlugin):
version = 0.1
shortName = 'Countdown'
inQueue = Queue()
outQueue = Queue()
updateTimer = 0.5
description = 'Count database objects down to zero'
outputType = SlideRunnerPlugin.PluginOutputType.NO_OVERLAY
pluginType = SlideRunnerPlugin.PluginTypes.WHOLESLIDE_PLUGIN
configurationList = list(
(SlideRunnerPlugin.PluginConfigurationEntry(uid=0,
name='Count down from',
initValue=300.,
minValue=0.0,
maxValue=1200.0), ))
def __init__(self, statusQueue: Queue):
self.statusQueue = statusQueue
self.p = Thread(target=self.queueWorker, daemon=True)
self.p.start()
self.total_number = 300
self.last_count = None
pass
def queueWorker(self):
quitSignal = False
while not quitSignal:
job = SlideRunnerPlugin.pluginJob(self.inQueue.get())
self.total_number = int(job.configuration[0])
if (job.jobDescription ==
SlideRunnerPlugin.JobDescription.QUIT_PLUGIN_THREAD):
# signal to exit this thread
quitSignal = True
continue
database = job.openedDatabase
annotations_count = len(database.annotations)
if annotations_count != self.last_count:
self.last_count = annotations_count
if self.total_number - annotations_count == 0:
self.setMessage('Done. Thanks for your help :)')
self.showMessageBox('Done. Thanks for your help :)')
elif self.total_number - annotations_count < 0:
self.setMessage(
'You are ambitious, thats gread, thank you')
else:
self.setMessage(
'{0} anotations to go'.format(self.total_number -
annotations_count))
def queueWorker(self):
quitSignal = False
while not quitSignal:
job = SlideRunnerPlugin.pluginJob(self.inQueue.get())
filename = job
if (job.jobDescription ==
SlideRunnerPlugin.JobDescription.QUIT_PLUGIN_THREAD):
# signal to exit this thread
quitSignal = True
continue
self.processWholeSlide(job)
def queueWorker(self):
self.targetModel = -1
self.lastCoordinates = ((None, None, None, None))
modelInitialized = False
oldThres = 0.0
print('Queue worker running.')
quitSignal = False
while not quitSignal:
job = SlideRunnerPlugin.pluginJob(self.inQueue.get())
if not (modelInitialized):
self.initializeModel()
modelInitialized = True
if (job.jobDescription ==
SlideRunnerPlugin.JobDescription.QUIT_PLUGIN_THREAD):
# signal to exit this thread
quitSignal = True
continue
print('Received request for: ', job.slideFilename, job.coordinates,
job.currentImage.shape)
updateAnnos = False
model = Models_Extensions[job.configuration[1]][1]
if not (model == self.targetModel):
self.targetModel = model
self.loadModel(self.targetModel, int(job.configuration[2]))
self.resultsStoreFilename = job.slideFilename + '_results_%s_%d.npz' % (
Models_Extensions[job.configuration[1]][0],
job.configuration[2])
if (self.slideFilename is None):
print('Processing complete slide ..')
self.processWholeSlide(job)
self.slideFilename = job.slideFilename
updateAnnos = True
if not (oldThres == job.configuration[0]):
updateAnnos = True
oldThres = job.configuration[0]
def queueWorker(self):
quitSignal = False
oldSlide = ''
while not quitSignal:
job = SlideRunnerPlugin.pluginJob(self.inQueue.get())
filename = job
if (job.jobDescription ==
SlideRunnerPlugin.JobDescription.QUIT_PLUGIN_THREAD):
# signal to exit this thread
quitSignal = True
continue
if (job.slideFilename != oldSlide) or (job.trigger is not None):
self.processWholeSlide(job)
oldSlide = job.slideFilename
else:
print('Trigger:', job.trigger)
def queueWorker(self):
self.targetModel = ''
self.targetEpoch = -1
self.lastCoordinates = ((None, None, None, None))
modelInitialized = False
print('Queue worker running.')
quitSignal = False
while not quitSignal:
job = SlideRunnerPlugin.pluginJob(self.inQueue.get())
if not (modelInitialized):
self.initializeModel()
modelInitialized = True
if (job.jobDescription ==
SlideRunnerPlugin.JobDescription.QUIT_PLUGIN_THREAD):
# signal to exit this thread
quitSignal = True
continue
print('Received request for: ', job.slideFilename, job.coordinates,
job.currentImage.shape)
model = Models_Extensions[job.configuration[2]][1]
if not (model == self.targetModel) and (self.targetEpoch
is not int(
job.configuration[3])):
if (self.loadModel(model, int(job.configuration[3]))):
self.targetModel = model
self.targetEpoch = int(job.configuration[3])
else:
self.targetModel = ''
self.targetEpoch = -1
self.EXTENSION = Models_Extensions[job.configuration[2]][0]
if (job.configuration[3] < 150):
self.EXTENSION += "_epoch%d" % int(job.configuration[3])
if (self.slideFilename is None):
print('Processing complete slide ..')
self.processWholeSlide(job)
self.slideFilename = job.slideFilename
def queueWorker(self):
quitSignal = False
while not quitSignal:
job = SlideRunnerPlugin.pluginJob(self.inQueue.get())
image = job.currentImage
if (job.jobDescription == SlideRunnerPlugin.JobDescription.QUIT_PLUGIN_THREAD):
# signal to exit this thread
quitSignal=True
continue
print('Macenko norm plugin: received 1 image from queue')
self.setProgressBar(0)
rgb = np.copy(image[:,:,0:3])
rgb = normalize(rgb)
self.returnImage(np.float32(rgb), job.procId)
self.setMessage('Macenko normalization: done.')
self.setProgressBar(-1)
class Plugin(SlideRunnerPlugin.SlideRunnerPlugin):
version = 0.1
shortName = 'Re-Stained WSI Registration (Jiang et al.)'
inQueue = Queue()
outQueue = Queue()
updateTimer=0.5
outputType = SlideRunnerPlugin.PluginOutputType.RGB_OVERLAY
description = 'Apply Jiang''s method for WSI co-registration'
pluginType = SlideRunnerPlugin.PluginTypes.IMAGE_PLUGIN
configurationList = list((
SlideRunnerPlugin.FilePickerConfigurationEntry(uid='file', name='Second WSI', mask='*.svs;;*.tif'),
SlideRunnerPlugin.PluginConfigurationEntry(uid='xoffset', name='X Offset', initValue=0, minValue=-2000, maxValue=2000.0),
SlideRunnerPlugin.PluginConfigurationEntry(uid='yoffset', name='Y Offset', initValue=0, minValue=-2000, maxValue=2000.0),
SlideRunnerPlugin.PushbuttonPluginConfigurationEntry(uid='match',name='Match')
))
def __init__(self, statusQueue:Queue):
self.statusQueue = statusQueue
self.p = Thread(target=self.queueWorker, daemon=True)
self.p.start()
pass
def queueWorker(self):
oldwsi=None
quitSignal=False
sl=None
detected_offset = None
sl_main = None
while not quitSignal:
job = SlideRunnerPlugin.pluginJob(self.inQueue.get())
image = job.currentImage
mainWSI = job.slideFilename
if 'file' not in job.configuration:
continue
if (job.configuration['file'] != oldwsi) and (job.configuration['file']!='') and job.configuration['file']:
sl = openslide.open_slide(job.configuration['file'])
if (mainWSI):
sl_main = openslide.open_slide(mainWSI)
if (job.trigger is not None) and job.trigger.uid=='match':
print('Trigger: ',job.trigger)
self.setProgressBar(0)
self.setMessage('Calculating optimum offset')
tissue_detector = TissueDetector("LAB_Threshold", threshold=80) # option 1
matcher_parameters = MatcherParameters() # use the default parameters
matcher = WSI_Matcher(tissue_detector, matcher_parameters)
detected_offset = matcher.match(mainWSI, job.configuration['file'])
self.setProgressBar(-1)
updateConfig = list()
updateConfig.append(SlideRunnerPlugin.PluginConfigUpdateEntry(SlideRunnerPlugin.PluginConfigurationType.SLIDER_WITH_FLOAT_VALUE, uid='xoffset', value=detected_offset[0]))
updateConfig.append(SlideRunnerPlugin.PluginConfigUpdateEntry(SlideRunnerPlugin.PluginConfigurationType.SLIDER_WITH_FLOAT_VALUE, uid='yoffset', value=detected_offset[1]))
self.updateConfiguration(SlideRunnerPlugin.PluginConfigUpdate(updateConfig))
if (sl) and (sl_main):
self.setProgressBar(0)
print('Reading from: ',job)
zoomValue=job.coordinates[3]/job.currentImage.shape[0]
print('Zoom value: ',zoomValue)
act_level = np.argmin(np.abs(np.asarray(sl.level_downsamples)-zoomValue))
closest_ds = sl_main.level_downsamples[np.argmin(np.abs(np.asarray(sl_main.level_downsamples)-zoomValue))]
offset = (job.configuration['xoffset'],job.configuration['yoffset'])
if (detected_offset is not None):
offset=detected_offset
offset_scaled = [int(x/closest_ds) for x in offset]
print('Scaled offset is: ',offset_scaled)
imgarea_w=job.coordinates[2:4]
size_im = (int(imgarea_w[0]/closest_ds), int(imgarea_w[1]/closest_ds))
print('Image size: ',size_im)
location = [int(x+y) for x,y in zip(job.coordinates[0:2],offset)]
print('Location (original):',job.coordinates[0:2])
print('Location (offset): ',location)
img = sl.read_region(location=location, level=act_level, size=size_im)
img = np.array(img.resize((job.currentImage.shape[1],job.currentImage.shape[0] )))
self.returnImage(img, job.procId)
self.setMessage('Align done.')
self.setProgressBar(-1)
if (job.jobDescription == SlideRunnerPlugin.JobDescription.QUIT_PLUGIN_THREAD):
# signal to exit this thread
quitSignal=True
continue
print('OTSU plugin: received 1 image from queue')
class Plugin(SlideRunnerPlugin.SlideRunnerPlugin):
version = 0.0
shortName = 'Mitosis Detection CMT'
inQueue = Queue()
outQueue = Queue()
description = 'Direct Regression of Mitotic Activity'
pluginType = SlideRunnerPlugin.PluginTypes.WHOLESLIDE_PLUGIN
outputType = SlideRunnerPlugin.PluginOutputType.BINARY_MASK
modelInitialized = False
updateTimer = 0.1
slideFilename = None
models = list()
for [dirname, model] in Models_Extensions:
models.append(model)
configurationList = list(
(SlideRunnerPlugin.PluginConfigurationEntry(uid=0,
name='Detection Threshold',
initValue=0.3,
minValue=0.3,
maxValue=1.0),
SlideRunnerPlugin.ComboboxPluginConfigurationEntry(uid=1,
name='Model',
options=models),
SlideRunnerPlugin.PluginConfigurationEntry(uid=2,
name='Epoch',
initValue=150.00,
minValue=1.0,
maxValue=150.0)))
def __init__(self, statusQueue: Queue):
self.statusQueue = statusQueue
self.preprocessQueue = Queue()
self.preprocessorOutQueue = Queue()
self.slide = None
self.p = Thread(target=self.queueWorker, daemon=True)
self.p.start()
self.currentModelCheckpoint = -1
self.pp = dict()
for k in range(10):
self.pp[k] = Thread(target=self.preprocessWorker, daemon=True)
self.pp[k].start()
def worldCoordinatesToGridcoordinates(self, x,
y) -> ((int, int), (int, int)):
tile_indices = (int(np.floor(x / TILESIZE_X)),
int(np.floor(y / TILESIZE_Y)))
onTile_coordinates = (int(np.mod(x, TILESIZE_X)),
int(np.mod(y, TILESIZE_Y)))
return (tile_indices, onTile_coordinates)
def gridCoordinatesToWorldCoordinates(
self, tile_indices: (int, int), onTile_coordinates: (int, int)
) -> (int, int):
return (int(tile_indices[0] * TILESIZE_X + onTile_coordinates[0]),
int(tile_indices[1] * TILESIZE_Y + onTile_coordinates[1]))
def conv_conv_pool(self,
input_,
n_filters,
training,
name,
pool=True,
activation=tf.nn.relu):
"""{Conv -> BN -> RELU}x2 -> {Pool, optional}
Args:
input_ (4-D Tensor): (batch_size, H, W, C)
n_filters (list): number of filters [int, int]
training (1-D Tensor): Boolean Tensor
name (str): name postfix
pool (bool): If True, MaxPool2D
activation: Activaion functions
Returns:
net: output of the Convolution operations
pool (optional): output of the max pooling operations
"""
net = input_
with tf.variable_scope("layer{}".format(name)):
for i, F in enumerate(n_filters):
net = tf.layers.conv2d(net,
F, (3, 3),
activation=None,
padding='same',
name="conv_{}".format(i + 1))
net = tf.layers.batch_normalization(net,
training=training,
name="bn_{}".format(i + 1))
net = activation(net, name="relu{}_{}".format(name, i + 1))
if pool is False:
return net
pool = tf.layers.max_pooling2d(net, (2, 2),
strides=(2, 2),
name="pool_{}".format(name))
return net, pool
def upsample_concat(self, inputA, input_B, name):
"""Upsample `inputA` and concat with `input_B`
Args:
input_A (4-D Tensor): (N, H, W, C)
input_B (4-D Tensor): (N, 2*H, 2*H, C2)
name (str): name of the concat operation
Returns:
output (4-D Tensor): (N, 2*H, 2*W, C + C2)
"""
upsample = self.upsampling_2D(inputA, size=(2, 2), name=name)
return tf.concat([upsample, input_B],
axis=-1,
name="concat_{}".format(name))
def upsampling_2D(self, tensor, name, size=(2, 2)):
"""Upsample/Rescale `tensor` by size
Args:
tensor (4-D Tensor): (N, H, W, C)
name (str): name of upsampling operations
size (tuple, optional): (height_multiplier, width_multiplier)
(default: (2, 2))
Returns:
output (4-D Tensor): (N, h_multiplier * H, w_multiplier * W, C)
"""
H, W, _ = tensor.get_shape().as_list()[1:]
H_multi, W_multi = size
target_H = H * H_multi
target_W = W * W_multi
return tf.image.resize_nearest_neighbor(
tensor, (target_H, target_W), name="upsample_{}".format(name))
def loadModel(self, modelpath, epoch):
pluginDir = os.path.dirname(os.path.realpath(__file__))
modelpath = pluginDir + os.sep + modelpath
modelFound = False
for dirpath, dirnames, files in os.walk(modelpath):
for fname in files:
realfname, ext = os.path.splitext(fname)
if ('_%d.ckpt' % epoch) in realfname:
if (modelpath + os.sep + realfname
is not self.currentModelCheckpoint):
try:
self.saver.restore(self.sess,
modelpath + os.sep + realfname)
print('Restored ', realfname)
modelFound = True
self.currentModelCheckpoint = modelpath + os.sep + realfname
except:
print('Unable to load', realfname)
exit()
if not modelFound:
print('Model not found in ', modelpath)
exit()
def initializeModel(self):
self.model = dict()
self.setMessage('DirReg init.')
self.setProgressBar(0)
tf.reset_default_graph()
self.netEpoch = tf.placeholder(tf.float32, name="epoch")
self.model['X'] = tf.placeholder(
tf.float32, shape=[None, IMAGESIZE_Y, IMAGESIZE_X, 3], name="X0")
self.model['y'] = tf.placeholder(
tf.float32, shape=[None, IMAGESIZE_Y, IMAGESIZE_X, 1], name="y")
self.model['mode'] = tf.placeholder(tf.bool, name="mode")
self.trainStep = tf.placeholder(tf.float32, name="trainStep")
self.model['pred'] = make_net(self.model['X'], self.model['mode'])
update_ops = tf.get_collection(tf.GraphKeys.UPDATE_OPS)
summary_op = tf.summary.merge_all()
self.sess = tf.Session()
print('Initializing variables')
init = tf.global_variables_initializer()
self.sess.run(init)
print('Done.')
self.saver = tf.train.Saver()
def queueWorker(self):
self.targetModel = -1
self.lastCoordinates = ((None, None, None, None))
modelInitialized = False
oldThres = 0.0
print('Queue worker running.')
quitSignal = False
while not quitSignal:
job = SlideRunnerPlugin.pluginJob(self.inQueue.get())
if not (modelInitialized):
self.initializeModel()
modelInitialized = True
if (job.jobDescription ==
SlideRunnerPlugin.JobDescription.QUIT_PLUGIN_THREAD):
# signal to exit this thread
quitSignal = True
continue
print('Received request for: ', job.slideFilename, job.coordinates,
job.currentImage.shape)
updateAnnos = False
model = Models_Extensions[job.configuration[1]][1]
if not (model == self.targetModel):
self.targetModel = model
self.loadModel(self.targetModel, int(job.configuration[2]))
self.resultsStoreFilename = job.slideFilename + '_results_%s_%d.npz' % (
Models_Extensions[job.configuration[1]][0],
job.configuration[2])
if (self.slideFilename is None):
print('Processing complete slide ..')
self.processWholeSlide(job)
self.slideFilename = job.slideFilename
updateAnnos = True
if not (oldThres == job.configuration[0]):
updateAnnos = True
oldThres = job.configuration[0]
def preprocessWorker(self):
while (True):
if self.preprocessorOutQueue.qsize() < 500:
(tile_x, tile_y, filename, coordinates,
tile_current) = self.preprocessQueue.get()
sl = openslide.open_slide(filename)
tn = sl.read_region(
location=(int(coordinates[0] - margin),
int(coordinates[1] - margin)),
level=0,
size=(int(coordinates[2] - coordinates[0] + 2 * margin),
int(coordinates[3] - coordinates[1] + 2 * margin)))
X_test = np.float32(
cv2.cvtColor(np.array(tn), cv2.COLOR_BGRA2RGB))[:, :, ::-1]
X_test = cv2.cvtColor(X_test, cv2.COLOR_BGR2RGB)
#X_test = np.reshape(X_test, newshape=[1,512,512,3])
self.preprocessorOutQueue.put(
(X_test, tile_x, tile_y, coordinates, tile_current))
else:
time.sleep(0.1)
def processWholeSlide(self, job):
self.setMessage('DirReg preparing ..')
self.setProgressBar(0)
filename = job.slideFilename
sl = openslide.open_slide(filename)
if os.path.isfile(self.resultsStoreFilename):
resultsStore = np.load(self.resultsStoreFilename)
self.tilesProcessed = resultsStore['tilesProcessed'].tolist()
self.scores = resultsStore['scores'].tolist()
else:
self.tilesProcessed = list()
self.scores = list()
self.slide = sl
tiles_total_x = int(np.floor(sl.dimensions[0] / TILESIZE_X))
tiles_total_y = int(np.floor(sl.dimensions[1] / TILESIZE_Y))
tiles_total = tiles_total_x * tiles_total_y
tiles2process_total = tile_current = 0
for tile_y in range(tiles_total_y):
for tile_x in range(tiles_total_x):
tile_current += 1
if ([tile_x, tile_y] in self.tilesProcessed):
continue
coords_p1 = self.gridCoordinatesToWorldCoordinates(
(tile_x, tile_y), (0, 0))
coords_p2 = self.gridCoordinatesToWorldCoordinates(
(tile_x, tile_y), (TILESIZE_X, TILESIZE_Y))
# out_fullscale = self.processTile(sl, coordinates=(coords_p1+coords_p2))
coordinates = coords_p1 + coords_p2
self.preprocessQueue.put(
(tile_x, tile_y, filename, coordinates, tile_current))
tiles2process_total += 1
out_tile_current = 0
batchsize = 20
batchcounter = 0
batch = np.zeros((batchsize, IMAGESIZE_Y, IMAGESIZE_X, 3))
batchTiles = list()
print('Images to be processed count: ', tiles2process_total)
import time
gt = 0
gtf = 0
out_tile_count = 0
while (out_tile_count < tiles2process_total):
t = time.time()
(X_test, tile_x, tile_y, coordinates,
out_tile_current) = self.preprocessorOutQueue.get()
gt += time.time() - t
batch[batchcounter] = X_test
batchTiles.append([tile_x, tile_y])
batchcounter += 1
out_tile_count += 1
if (batchcounter == batchsize) or (out_tile_count
== tiles2process_total):
gtf -= time.time()
out = self.evaluateImage(batch[0:batchcounter])
gtf += time.time()
# print('TensorFlow: %.2f s, Preproc: %.2f s, QSize: %d ' % (gtf, gt, self.preprocessorOutQueue.qsize()))
for k in range(out.shape[0]):
self.scores.append(out[k])
self.tilesProcessed += batchTiles
print('Length of out:', len(self.scores),
len(self.tilesProcessed))
if (len(self.scores) != len(self.tilesProcessed)):
print('Wrong length!')
sys.exit()
batchTiles = list()
batchcounter = 0
self.setProgressBar(100 * out_tile_count / tiles_total)
# split into chunks
if (self.inQueue.qsize() > 0): # new request pending
self.saveState()
return
print('Length of out:', len(self.scores), len(self.tilesProcessed),
out_tile_count)
self.saveState()
self.setProgressBar(-1)
self.setMessage('DirReg calculation done.')
def saveState(self):
np.savez_compressed(self.resultsStoreFilename,
scores=self.scores,
tilesProcessed=self.tilesProcessed)
def exceptionHandlerOnExit(self):
if (self.slide):
self.saveState()
def getAnnotations(self):
return self.annos
def evaluateImage(self, image):
# if (np.all(np.mean(image, axis=(1,2,3))<200)):
[y_act] = self.sess.run([self.model['pred']],
feed_dict={
self.model['X']: image,
self.model['mode']: False
})
return y_act
class Plugin(SlideRunnerPlugin.SlideRunnerPlugin):
version = 0.0
shortName = 'Mitosis UNET offline (model select, downsampled)'
inQueue = Queue()
outQueue = Queue()
description = 'Find mitotic figures using UNET'
pluginType = SlideRunnerPlugin.PluginTypes.WHOLESLIDE_PLUGIN
outputType = SlideRunnerPlugin.PluginOutputType.BINARY_MASK
modelInitialized = False
updateTimer = 1.0
slideFilename = None
models = list()
for [dirname, model] in Models_Extensions:
models.append(model)
configurationList = list((
SlideRunnerPlugin.PluginConfigurationEntry(uid=0,
name='Density Radius',
initValue=0.00,
minValue=0.0,
maxValue=255.0),
SlideRunnerPlugin.PluginConfigurationEntry(uid=1,
name='Amplification',
initValue=4.00,
minValue=1.0,
maxValue=10.0),
SlideRunnerPlugin.ComboboxPluginConfigurationEntry(uid=2,
name='Model',
options=models),
SlideRunnerPlugin.PluginConfigurationEntry(uid=3,
name='Epoch',
initValue=134.00,
minValue=130.0,
maxValue=150.0),
))
def __init__(self, statusQueue: Queue):
self.statusQueue = statusQueue
self.preprocessQueue = Queue()
self.preprocessorOutQueue = Queue()
self.p = Thread(target=self.queueWorker, daemon=True)
self.p.start()
self.EXTENSION = ''
self.pp = dict()
self.lastPlugin = -1
for k in range(5):
self.pp[k] = Thread(target=self.preprocessWorker, daemon=True)
self.pp[k].start()
def conv_conv_pool(self,
input_,
n_filters,
training,
name,
pool=True,
activation=tf.nn.relu):
"""{Conv -> BN -> RELU}x2 -> {Pool, optional}
Args:
input_ (4-D Tensor): (batch_size, H, W, C)
n_filters (list): number of filters [int, int]
training (1-D Tensor): Boolean Tensor
name (str): name postfix
pool (bool): If True, MaxPool2D
activation: Activaion functions
Returns:
net: output of the Convolution operations
pool (optional): output of the max pooling operations
"""
net = input_
with tf.variable_scope("layer{}".format(name)):
for i, F in enumerate(n_filters):
net = tf.layers.conv2d(net,
F, (3, 3),
activation=None,
padding='same',
name="conv_{}".format(i + 1))
net = tf.layers.batch_normalization(net,
training=training,
name="bn_{}".format(i + 1))
net = activation(net, name="relu{}_{}".format(name, i + 1))
if pool is False:
return net
pool = tf.layers.max_pooling2d(net, (2, 2),
strides=(2, 2),
name="pool_{}".format(name))
return net, pool
def upsample_concat(self, inputA, input_B, name):
"""Upsample `inputA` and concat with `input_B`
Args:
input_A (4-D Tensor): (N, H, W, C)
input_B (4-D Tensor): (N, 2*H, 2*H, C2)
name (str): name of the concat operation
Returns:
output (4-D Tensor): (N, 2*H, 2*W, C + C2)
"""
upsample = self.upsampling_2D(inputA, size=(2, 2), name=name)
return tf.concat([upsample, input_B],
axis=-1,
name="concat_{}".format(name))
def upsampling_2D(self, tensor, name, size=(2, 2)):
"""Upsample/Rescale `tensor` by size
Args:
tensor (4-D Tensor): (N, H, W, C)
name (str): name of upsampling operations
size (tuple, optional): (height_multiplier, width_multiplier)
(default: (2, 2))
Returns:
output (4-D Tensor): (N, h_multiplier * H, w_multiplier * W, C)
"""
H, W, _ = tensor.get_shape().as_list()[1:]
H_multi, W_multi = size
target_H = H * H_multi
target_W = W * W_multi
return tf.image.resize_nearest_neighbor(
tensor, (target_H, target_W), name="upsample_{}".format(name))
def make_unet(self, Xhinted, training):
"""Build a U-Net architecture
Args:
X (4-D Tensor): (N, H, W, C)
training (1-D Tensor): Boolean Tensor is required for batchnormalization layers
Returns:
output (4-D Tensor): (N, H, W, C)
Same shape as the `input` tensor
Notes:
U-Net: Convolutional Networks for Biomedical Image Segmentation
https://arxiv.org/abs/1505.04597
"""
net = Xhinted / 127.5 - 1
net = tf.layers.conv2d(net, 4, (1, 1), name="color_space_adjust")
conv1, pool1 = self.conv_conv_pool(net, [8, 8], training, name=1)
conv2, pool2 = self.conv_conv_pool(pool1, [16, 16], training, name=2)
conv3, pool3 = self.conv_conv_pool(pool2, [32, 32], training, name=3)
conv4, pool4 = self.conv_conv_pool(pool3, [64, 64], training, name=4)
conv5 = self.conv_conv_pool(pool4, [128, 128],
training,
name=5,
pool=False)
up6 = self.upsample_concat(conv5, conv4, name=6)
conv6 = self.conv_conv_pool(up6, [64, 64],
training,
name=6,
pool=False)
up7 = self.upsample_concat(conv6, conv3, name=7)
conv7 = self.conv_conv_pool(up7, [32, 32],
training,
name=7,
pool=False)
up8 = self.upsample_concat(conv7, conv2, name=8)
conv8 = self.conv_conv_pool(up8, [16, 16],
training,
name=8,
pool=False)
up9 = self.upsample_concat(conv8, conv1, name=9)
conv9 = self.conv_conv_pool(up9, [8, 8], training, name=9, pool=False)
return tf.layers.conv2d(conv9,
1, (1, 1),
name='final',
activation=tf.nn.sigmoid,
padding='same')
def initializeModel(self):
self.model = dict()
tf.reset_default_graph()
self.netEpoch = tf.placeholder(tf.float32, name="epoch")
self.model['X'] = tf.placeholder(
tf.float32, shape=[None, IMAGESIZE_Y, IMAGESIZE_X, 3], name="X0")
self.model['y'] = tf.placeholder(
tf.float32, shape=[None, IMAGESIZE_Y, IMAGESIZE_X, 1], name="y")
self.model['mode'] = tf.placeholder(tf.bool, name="mode")
self.trainStep = tf.placeholder(tf.float32, name="trainStep")
self.model['pred'] = self.make_unet(self.model['X'],
self.model['mode'])
pluginDir = os.path.dirname(os.path.realpath(__file__))
update_ops = tf.get_collection(tf.GraphKeys.UPDATE_OPS)
summary_op = tf.summary.merge_all()
self.sess = tf.Session()
print('Initializing variables')
init = tf.global_variables_initializer()
self.sess.run(init)
print('Done.')
self.currentModelCheckpoint = ''
self.saver = tf.train.Saver()
def loadModel(self, modelpath, epoch):
pluginDir = os.path.dirname(os.path.realpath(__file__))
modelpath = pluginDir + os.sep + modelpath
modelFound = False
for dirpath, dirnames, files in os.walk(modelpath):
for fname in files:
realfname, ext = os.path.splitext(fname)
if ('_%d.ckpt' % epoch) in realfname:
if (modelpath + os.sep + realfname
is not self.currentModelCheckpoint):
try:
self.saver.restore(self.sess,
modelpath + os.sep + realfname)
print('Restored ', realfname)
modelFound = True
self.currentModelCheckpoint = modelpath + os.sep + realfname
except:
print('Unable to load', realfname, 'in', modelpath)
exit()
if not modelFound:
print('Model not found in ', modelpath)
self.setMessage('Model not found in' + str(modelpath))
return False
return True
def queueWorker(self):
self.targetModel = ''
self.targetEpoch = -1
self.lastCoordinates = ((None, None, None, None))
modelInitialized = False
print('Queue worker running.')
quitSignal = False
while not quitSignal:
job = SlideRunnerPlugin.pluginJob(self.inQueue.get())
if not (modelInitialized):
self.initializeModel()
modelInitialized = True
if (job.jobDescription ==
SlideRunnerPlugin.JobDescription.QUIT_PLUGIN_THREAD):
# signal to exit this thread
quitSignal = True
continue
print('Received request for: ', job.slideFilename, job.coordinates,
job.currentImage.shape)
model = Models_Extensions[job.configuration[2]][1]
if not (model == self.targetModel) and (self.targetEpoch
is not int(
job.configuration[3])):
if (self.loadModel(model, int(job.configuration[3]))):
self.targetModel = model
self.targetEpoch = int(job.configuration[3])
else:
self.targetModel = ''
self.targetEpoch = -1
self.EXTENSION = Models_Extensions[job.configuration[2]][0]
if (job.configuration[3] < 150):
self.EXTENSION += "_epoch%d" % int(job.configuration[3])
if (self.slideFilename is None):
print('Processing complete slide ..')
self.processWholeSlide(job)
self.slideFilename = job.slideFilename
def preprocessWorker(self):
while (True):
if self.preprocessorOutQueue.qsize() < 100:
(tile_x, tile_y, sl, coordinates,
tile_current) = self.preprocessQueue.get()
try:
tn = sl.read_region(
location=(int(coordinates[0] - margin),
int(coordinates[1] - margin)),
level=0,
size=(int(coordinates[2] - coordinates[0] +
2 * margin),
int(coordinates[3] - coordinates[1] +
2 * margin)))
X_test = np.float32(
cv2.cvtColor(np.array(tn),
cv2.COLOR_BGRA2RGB))[:, :, ::-1]
self.preprocessorOutQueue.put(
(X_test, tile_x, tile_y, coordinates, tile_current))
except Exception as e:
self.preprocessQueue.put(
(tile_x, tile_y, sl, coordinates, tile_current))
print('Did not work, message is: ', e)
print('Tiles: ', tile_x, tile_y)
print('Coordinates:')
print('Slide dimensions:', sl.dimensions)
print('Coordinates are:',
(int(coordinates[0] - margin), int(coordinates[1])),
(int(coordinates[2] - coordinates[0] + 2 * margin),
int(coordinates[3] - coordinates[1] + 1 * margin)))
else:
time.sleep(0.1)
def worldCoordinatesToGridcoordinates(self, x,
y) -> ((int, int), (int, int)):
tile_indices = (int(np.floor(x / TILESIZE_X)),
int(np.floor(y / TILESIZE_Y)))
onTile_coordinates = (int(np.mod(x, TILESIZE_X)),
int(np.mod(y, TILESIZE_Y)))
return (tile_indices, onTile_coordinates)
def gridCoordinatesToWorldCoordinates(
self, tile_indices: (int, int), onTile_coordinates: (int, int)
) -> (int, int):
return (int(tile_indices[0] * TILESIZE_X + onTile_coordinates[0]),
int(tile_indices[1] * TILESIZE_Y + onTile_coordinates[1]))
def processWholeSlide(self, job):
filename = job.slideFilename
sl = openslide.open_slide(filename)
ds = 32
self.imageMap = np.zeros(
(int(sl.dimensions[1] / ds), int(sl.dimensions[0] / ds)))
self.slide = sl
tiles_total_x = int(np.ceil(sl.dimensions[0] / TILESIZE_X))
tiles_total_y = int(np.ceil(sl.dimensions[1] / TILESIZE_Y))
tiles_total = tiles_total_x * tiles_total_y
basefilename = job.slideFilename + self.EXTENSION + '_UNET.npz'
if not os.path.exists(basefilename) and (self.targetModel is not ''):
tiles2process_total = tile_current = 0
for tile_y in range(tiles_total_y):
for tile_x in range(tiles_total_x):
tile_current += 1
coords_p1 = self.gridCoordinatesToWorldCoordinates(
(tile_x, tile_y), (0, 0))
coords_p2 = self.gridCoordinatesToWorldCoordinates(
(tile_x, tile_y), (TILESIZE_X, TILESIZE_Y))
# out_fullscale = self.processTile(sl, coordinates=(coords_p1+coords_p2))
coordinates = coords_p1 + coords_p2
self.preprocessQueue.put(
(tile_x, tile_y, sl, coordinates, tile_current))
tiles2process_total += 1
out_tile_current = 0
out_tile_num = 0
ds = 32
print('Image size:', sl.dimensions)
print('Images to be processed count: ', tiles2process_total)
while (out_tile_num < tiles2process_total):
out_tile_num += 1
(X_test, tile_x, tile_y, coordinates,
out_tile_current) = self.preprocessorOutQueue.get()
out_fullscale = self.evaluateImage(X_test)
target_size = (int(TILESIZE_Y / ds), int(TILESIZE_X / ds))
out_ds = cv2.resize(out_fullscale, dsize=(target_size))
target_x = int(coordinates[0] / ds)
target_y = int(coordinates[1] / ds)
#print('from coord: ',coordinates, target_x, target_y)
if (coordinates[0] + IMAGESIZE_X < sl.dimensions[0]) and (
coordinates[1] + IMAGESIZE_Y < sl.dimensions[1]):
self.imageMap[target_y:target_y + target_size[0],
target_x:target_x + target_size[1]] = out_ds
else:
remainsize = self.imageMap[target_y:target_y +
target_size[0],
target_x:target_x +
target_size[1]].shape
self.imageMap[target_y:target_y + target_size[0],
target_x:target_x +
target_size[1]] = out_ds[0:remainsize[0],
0:remainsize[1]]
self.setProgressBar(100 * out_tile_num / tiles_total)
np.savez(basefilename, imageMap=self.imageMap)
elif os.path.exists(basefilename):
f = np.load(basefilename)
self.imageMap = f['imageMap']
else:
self.setMessage('No cache and no model found.')
x, y, w, h = job.coordinates
retImg = cv2.getRectSubPix(np.float32(np.copy(self.imageMap)),
(int(w / ds), int(h / ds)),
center=((x - 0.5 * w) / ds,
(y - 0.5 * h) / ds))
retImg = cv2.resize(retImg,
dsize=(job.currentImage.shape[1],
job.currentImage.shape[0]))
self.returnImage(retImg, job.procId)
self.setProgressBar(-1)
def evaluateImage(self, image):
import cv2
overlap = 128
margin = 64
gridCoords_relative = list()
# split into chunks
X_test = cv2.cvtColor(image, cv2.COLOR_BGR2RGB)
X_test = np.reshape(X_test, newshape=[1, 512, 512, 3])
y_est = self.sess.run([self.model['pred']],
feed_dict={
self.model['X']: X_test,
self.model['mode']: False
})
y_est = np.float32(np.asarray(y_est).squeeze())
mapOut = y_est[margin:IMAGESIZE_Y - margin,
margin:IMAGESIZE_X - margin]
return mapOut
def queueWorker(self):
debugModule = False
quitSignal = False
oldFilename = ''
oldArchive = ''
oldSlide = ''
oldDBfile = ''
oldCoordinates = [-1, -1, -1, -1]
oldThres = -1
oldSource = -1
self.ds = 32
while not quitSignal:
job = SlideRunnerPlugin.pluginJob(self.inQueue.get())
print(job)
print(job.configuration)
if (job.jobDescription ==
SlideRunnerPlugin.JobDescription.QUIT_PLUGIN_THREAD):
# signal to exit this thread
quitSignal = True
continue
sourceChanged = job.configuration['source'] != oldSource
if (job.configuration['source'] == 0):
if not hasattr(job.openedDatabase, 'dbfilename'):
# DB not open yet
continue
job.configuration['dbfile'] = job.openedDatabase.dbfilename
dbfilechanged = job.configuration['dbfile'] != oldDBfile
if not (sourceChanged) and (
job.configuration['file'] == oldArchive
) and (job.configuration['threshold']
== oldThres) and (job.slideFilename == oldSlide) and np.all(
job.coordinates == oldCoordinates) and not (
dbfilechanged):
continue
if not (os.path.exists(job.configuration['file'])) and (
job.configuration['source'] == 1):
continue
self.sendAnnotationLabelUpdate()
fileChanged = job.configuration['file'] != oldArchive
oldDBfile = job.configuration['dbfile']
slideChanged = job.slideFilename != oldSlide
thresChanged = job.configuration['threshold'] != oldThres
oldArchive = job.configuration['file']
oldThres = job.configuration['threshold']
oldSlide = job.slideFilename
oldSource = job.configuration['source']
oldCoordinates = job.coordinates
[foo, self.ext] = os.path.splitext(oldArchive)
self.ext = self.ext.upper()
self.slideObj = openslide.open_slide(job.slideFilename)
if (fileChanged):
if (self.ext == '.P') or (
self.ext
== '.BZ2'): # Pickled format - results for many slides
if (self.ext == '.BZ2'):
self.resultsArchive = pickle.load(
bz2.BZ2File(oldArchive, 'rb'))
print('Opened bz2-compressed results container.')
else:
self.resultsArchive = pickle.load(
open(oldArchive, 'rb'))
print('Sourcechanged:', sourceChanged, 'dbfilechanged:',
dbfilechanged, (len(job.configuration['dbfile']) > 0))
if (sourceChanged or dbfilechanged or slideChanged) and (
(job.configuration['source'] == 2) or
(job.configuration['source']
== 0)) and (len(job.configuration['dbfile']) > 0):
self.slideObj = openslide.open_slide(job.slideFilename)
self.downsampledMap = np.zeros(
(int(self.slideObj.dimensions[1] / self.ds),
int(self.slideObj.dimensions[0] / self.ds)))
self.newDB = Database()
self.newDB.open(job.configuration['dbfile'])
allClasses = self.newDB.getAllClasses()
mitosisClass = -1
for clsname, clsuid, col in allClasses:
if (mitosisClass
== -1) and ('MITO' in clsname.upper()) and (
'LOOK' not in clsname.upper()):
mitosisClass = clsuid
pname, fname = os.path.split(job.slideFilename)
uid = self.newDB.findSlideWithFilename(fname, pname)
self.newDB.loadIntoMemory(uid)
for anno in self.newDB.annotations:
if (self.newDB.annotations[anno].agreedClass ==
mitosisClass):
annodet = self.newDB.annotations[anno]
self.downsampledMap[int(annodet.y1 / self.ds),
int(annodet.x1 / self.ds)] += 1
else:
self.setMessage('No DB open.')
if ((sourceChanged and job.configuration['source'] == 1) or
(slideChanged) or
(thresChanged)) and len(job.configuration['file']) > 0:
pname, fname = os.path.split(job.slideFilename)
print('Stage 6')
if (oldFilename is not fname) or (slideChanged):
# process slide
self.annos = list()
if (fname not in self.resultsArchive):
self.setMessage('Slide ' + str(fname) +
' not found in results file.')
print('List of files is: ', self.resultsArchive.keys())
continue
oldFilename = fname
uniqueLabels = np.unique(
np.array(self.resultsArchive[fname])[:, 4])
self.annotationLabels = dict()
for key, label in enumerate(uniqueLabels):
self.annotationLabels[
label] = SlideRunnerPlugin.PluginAnnotationLabel(
0, 'Class %d' % label,
self.COLORS[key % len(self.COLORS)])
if (job.configuration['source'] == 1):
self.downsampledMap = np.zeros(
(int(self.slideObj.dimensions[1] / self.ds),
int(self.slideObj.dimensions[0] / self.ds)))
print('Downsampled image: ', self.downsampledMap.shape)
for idx in range(len(self.resultsArchive[fname])):
row = self.resultsArchive[fname][idx]
if (row[5] > job.configuration['threshold']):
myanno = annotations.rectangularAnnotation(
uid=idx,
x1=row[0],
x2=row[2],
y1=row[1],
y2=row[3],
text='%.2f' % row[5],
pluginAnnotationLabel=self.annotationLabels[
row[4]])
if (job.configuration['source'] == 1):
self.downsampledMap[int(
(row[1] + row[3]) / 2 / self.ds),
int((row[0] + row[2]) / 2 /
self.ds)] += 1
self.annos.append(myanno)
self.sendAnnotationLabelUpdate()
elif (self.ext == '.TXT'): # Assume MS Coco format
self.annos = list()
self.resultsArchive = np.loadtxt(
oldArchive,
dtype={
'names':
('label', 'confidence', 'x', 'y', 'w', 'h'),
'formats': ('U30', 'f4', 'i4', 'i4', 'i4', 'i4')
},
skiprows=0,
delimiter=' ')
uniqueLabels = np.unique(self.resultsArchive['label'])
self.annotationLabels = dict()
for key, label in enumerate(uniqueLabels):
self.annotationLabels[
label] = SlideRunnerPlugin.PluginAnnotationLabel(
0, label, self.COLORS[key % len(self.COLORS)])
self.sendAnnotationLabelUpdate()
self.slideObj = openslide.open_slide(job.slideFilename)
self.ds = 32
if (job.configuration['source'] == 1):
self.downsampledMap = np.zeros(
(int(self.slideObj.dimensions[1] / self.ds),
int(self.slideObj.dimensions[0] / self.ds)))
print('Downsampled image: ', self.downsampledMap.shape)
for idx in range(len(self.resultsArchive)):
row = self.resultsArchive[idx]
if (row[5] > job.configuration['threshold']):
if (job.configuration['source'] == 1):
self.downsampledMap[int(
(row['y'] - row['h'] / 2) / self.ds),
int((row['x'] -
row['w'] / 2) /
self.ds)] += 1
myanno = annotations.rectangularAnnotation(
uid=idx,
x1=row['x'],
x2=row['y'],
y1=row['x'] + row['w'],
y2=row['y'] + row['h'],
text='%.2f' % row['confidence'],
pluginAnnotationLabel=self.annotationLabels[
row['label']])
self.annos.append(myanno)
print('returning overlay...')
A = 2.37 # mm^2
W_hpf_microns = np.sqrt(A * 4 / 3) * 1000 # in microns
H_hpf_microns = np.sqrt(A * 3 / 4) * 1000 # in microns
micronsPerPixel = self.slideObj.properties[
openslide.PROPERTY_NAME_MPP_X]
W_hpf = int(W_hpf_microns / float(micronsPerPixel))
H_hpf = int(H_hpf_microns / float(micronsPerPixel))
W_x = int(W_hpf / self.ds)
W_y = int(H_hpf / self.ds)
kernel = np.ones((W_y, W_x), np.float32)
mitoticCount = cv2.filter2D(self.downsampledMap, -1, kernel)
coords_ds = np.int16(np.array(job.coordinates) / self.ds)
centerImg = cv2.getRectSubPix(
np.float32(mitoticCount[:, :, None]),
patchSize=(coords_ds[2], coords_ds[3]),
center=(coords_ds[0] + coords_ds[2] * 0.5,
coords_ds[1] + coords_ds[3] * 0.5))
resized = cv2.resize(centerImg,
dsize=(job.currentImage.shape[1],
job.currentImage.shape[0]))
self.returnImage(resized)
self.updateAnnotations()
self.setProgressBar(-1)
self.setMessage('found %d annotations.' % len(self.annos))
class Plugin(SlideRunnerPlugin.SlideRunnerPlugin):
version = 0.1
shortName = 'Mitosis Heatmap'
inQueue = Queue()
outQueue = Queue()
initialOpacity = 0.6
updateTimer = 0.1
outputType = SlideRunnerPlugin.PluginOutputType.BINARY_MASK
description = 'Show heatmap of mitotic figures in WSI'
pluginType = SlideRunnerPlugin.PluginTypes.WHOLESLIDE_PLUGIN
configurationList = list(
(SlideRunnerPlugin.FilePickerConfigurationEntry(
uid='file', name='Result file', mask='*.p;;*.txt;;*.p.bz2'),
SlideRunnerPlugin.FilePickerConfigurationEntry(uid='dbfile',
name='Database file',
mask='*.sqlite'),
SlideRunnerPlugin.PluginConfigurationEntry(uid='threshold',
name='Detection threshold',
initValue=0.75,
minValue=0.0,
maxValue=1.0),
SlideRunnerPlugin.ComboboxPluginConfigurationEntry(
uid='source',
name='Heatmap shows',
options=['Primary Database', 'Results', 'SecondaryDatabase'])))
COLORS = [[0, 128, 0, 255], [128, 0, 0, 255], [0, 0, 128, 255],
[128, 128, 0, 255], [0, 128, 128, 255], [128, 128, 128, 255]]
def __init__(self, statusQueue: Queue):
self.statusQueue = statusQueue
self.annotationLabels = {
'Detection':
SlideRunnerPlugin.PluginAnnotationLabel(0, 'Detection',
[0, 180, 0, 255]),
}
self.p = Thread(target=self.queueWorker, daemon=True)
self.p.start()
self.annos = []
self.downsampledMap = np.zeros((10, 10))
pass
def getAnnotationUpdatePolicy():
# This is important to tell SlideRunner that he needs to update for every change in position.
return SlideRunnerPlugin.AnnotationUpdatePolicy.UPDATE_ON_SLIDE_CHANGE
def queueWorker(self):
debugModule = False
quitSignal = False
oldFilename = ''
oldArchive = ''
oldSlide = ''
oldDBfile = ''
oldCoordinates = [-1, -1, -1, -1]
oldThres = -1
oldSource = -1
self.ds = 32
while not quitSignal:
job = SlideRunnerPlugin.pluginJob(self.inQueue.get())
print(job)
print(job.configuration)
if (job.jobDescription ==
SlideRunnerPlugin.JobDescription.QUIT_PLUGIN_THREAD):
# signal to exit this thread
quitSignal = True
continue
sourceChanged = job.configuration['source'] != oldSource
if (job.configuration['source'] == 0):
if not hasattr(job.openedDatabase, 'dbfilename'):
# DB not open yet
continue
job.configuration['dbfile'] = job.openedDatabase.dbfilename
dbfilechanged = job.configuration['dbfile'] != oldDBfile
if not (sourceChanged) and (
job.configuration['file'] == oldArchive
) and (job.configuration['threshold']
== oldThres) and (job.slideFilename == oldSlide) and np.all(
job.coordinates == oldCoordinates) and not (
dbfilechanged):
continue
if not (os.path.exists(job.configuration['file'])) and (
job.configuration['source'] == 1):
continue
self.sendAnnotationLabelUpdate()
fileChanged = job.configuration['file'] != oldArchive
oldDBfile = job.configuration['dbfile']
slideChanged = job.slideFilename != oldSlide
thresChanged = job.configuration['threshold'] != oldThres
oldArchive = job.configuration['file']
oldThres = job.configuration['threshold']
oldSlide = job.slideFilename
oldSource = job.configuration['source']
oldCoordinates = job.coordinates
[foo, self.ext] = os.path.splitext(oldArchive)
self.ext = self.ext.upper()
self.slideObj = openslide.open_slide(job.slideFilename)
if (fileChanged):
if (self.ext == '.P') or (
self.ext
== '.BZ2'): # Pickled format - results for many slides
if (self.ext == '.BZ2'):
self.resultsArchive = pickle.load(
bz2.BZ2File(oldArchive, 'rb'))
print('Opened bz2-compressed results container.')
else:
self.resultsArchive = pickle.load(
open(oldArchive, 'rb'))
print('Sourcechanged:', sourceChanged, 'dbfilechanged:',
dbfilechanged, (len(job.configuration['dbfile']) > 0))
if (sourceChanged or dbfilechanged or slideChanged) and (
(job.configuration['source'] == 2) or
(job.configuration['source']
== 0)) and (len(job.configuration['dbfile']) > 0):
self.slideObj = openslide.open_slide(job.slideFilename)
self.downsampledMap = np.zeros(
(int(self.slideObj.dimensions[1] / self.ds),
int(self.slideObj.dimensions[0] / self.ds)))
self.newDB = Database()
self.newDB.open(job.configuration['dbfile'])
allClasses = self.newDB.getAllClasses()
mitosisClass = -1
for clsname, clsuid, col in allClasses:
if (mitosisClass
== -1) and ('MITO' in clsname.upper()) and (
'LOOK' not in clsname.upper()):
mitosisClass = clsuid
pname, fname = os.path.split(job.slideFilename)
uid = self.newDB.findSlideWithFilename(fname, pname)
self.newDB.loadIntoMemory(uid)
for anno in self.newDB.annotations:
if (self.newDB.annotations[anno].agreedClass ==
mitosisClass):
annodet = self.newDB.annotations[anno]
self.downsampledMap[int(annodet.y1 / self.ds),
int(annodet.x1 / self.ds)] += 1
else:
self.setMessage('No DB open.')
if ((sourceChanged and job.configuration['source'] == 1) or
(slideChanged) or
(thresChanged)) and len(job.configuration['file']) > 0:
pname, fname = os.path.split(job.slideFilename)
print('Stage 6')
if (oldFilename is not fname) or (slideChanged):
# process slide
self.annos = list()
if (fname not in self.resultsArchive):
self.setMessage('Slide ' + str(fname) +
' not found in results file.')
print('List of files is: ', self.resultsArchive.keys())
continue
oldFilename = fname
uniqueLabels = np.unique(
np.array(self.resultsArchive[fname])[:, 4])
self.annotationLabels = dict()
for key, label in enumerate(uniqueLabels):
self.annotationLabels[
label] = SlideRunnerPlugin.PluginAnnotationLabel(
0, 'Class %d' % label,
self.COLORS[key % len(self.COLORS)])
if (job.configuration['source'] == 1):
self.downsampledMap = np.zeros(
(int(self.slideObj.dimensions[1] / self.ds),
int(self.slideObj.dimensions[0] / self.ds)))
print('Downsampled image: ', self.downsampledMap.shape)
for idx in range(len(self.resultsArchive[fname])):
row = self.resultsArchive[fname][idx]
if (row[5] > job.configuration['threshold']):
myanno = annotations.rectangularAnnotation(
uid=idx,
x1=row[0],
x2=row[2],
y1=row[1],
y2=row[3],
text='%.2f' % row[5],
pluginAnnotationLabel=self.annotationLabels[
row[4]])
if (job.configuration['source'] == 1):
self.downsampledMap[int(
(row[1] + row[3]) / 2 / self.ds),
int((row[0] + row[2]) / 2 /
self.ds)] += 1
self.annos.append(myanno)
self.sendAnnotationLabelUpdate()
elif (self.ext == '.TXT'): # Assume MS Coco format
self.annos = list()
self.resultsArchive = np.loadtxt(
oldArchive,
dtype={
'names':
('label', 'confidence', 'x', 'y', 'w', 'h'),
'formats': ('U30', 'f4', 'i4', 'i4', 'i4', 'i4')
},
skiprows=0,
delimiter=' ')
uniqueLabels = np.unique(self.resultsArchive['label'])
self.annotationLabels = dict()
for key, label in enumerate(uniqueLabels):
self.annotationLabels[
label] = SlideRunnerPlugin.PluginAnnotationLabel(
0, label, self.COLORS[key % len(self.COLORS)])
self.sendAnnotationLabelUpdate()
self.slideObj = openslide.open_slide(job.slideFilename)
self.ds = 32
if (job.configuration['source'] == 1):
self.downsampledMap = np.zeros(
(int(self.slideObj.dimensions[1] / self.ds),
int(self.slideObj.dimensions[0] / self.ds)))
print('Downsampled image: ', self.downsampledMap.shape)
for idx in range(len(self.resultsArchive)):
row = self.resultsArchive[idx]
if (row[5] > job.configuration['threshold']):
if (job.configuration['source'] == 1):
self.downsampledMap[int(
(row['y'] - row['h'] / 2) / self.ds),
int((row['x'] -
row['w'] / 2) /
self.ds)] += 1
myanno = annotations.rectangularAnnotation(
uid=idx,
x1=row['x'],
x2=row['y'],
y1=row['x'] + row['w'],
y2=row['y'] + row['h'],
text='%.2f' % row['confidence'],
pluginAnnotationLabel=self.annotationLabels[
row['label']])
self.annos.append(myanno)
print('returning overlay...')
A = 2.37 # mm^2
W_hpf_microns = np.sqrt(A * 4 / 3) * 1000 # in microns
H_hpf_microns = np.sqrt(A * 3 / 4) * 1000 # in microns
micronsPerPixel = self.slideObj.properties[
openslide.PROPERTY_NAME_MPP_X]
W_hpf = int(W_hpf_microns / float(micronsPerPixel))
H_hpf = int(H_hpf_microns / float(micronsPerPixel))
W_x = int(W_hpf / self.ds)
W_y = int(H_hpf / self.ds)
kernel = np.ones((W_y, W_x), np.float32)
mitoticCount = cv2.filter2D(self.downsampledMap, -1, kernel)
coords_ds = np.int16(np.array(job.coordinates) / self.ds)
centerImg = cv2.getRectSubPix(
np.float32(mitoticCount[:, :, None]),
patchSize=(coords_ds[2], coords_ds[3]),
center=(coords_ds[0] + coords_ds[2] * 0.5,
coords_ds[1] + coords_ds[3] * 0.5))
resized = cv2.resize(centerImg,
dsize=(job.currentImage.shape[1],
job.currentImage.shape[0]))
self.returnImage(resized)
self.updateAnnotations()
self.setProgressBar(-1)
self.setMessage('found %d annotations.' % len(self.annos))
def getAnnotations(self):
return self.annos
def getAnnotationLabels(self):
# sending default annotation labels
return [self.annotationLabels[k] for k in self.annotationLabels.keys()]
def queueWorker(self):
debugModule = False
quitSignal = False
oldFilename = ''
oldArchive = ''
oldSlide = ''
oldThres = -1
while not quitSignal:
job = SlideRunnerPlugin.pluginJob(self.inQueue.get())
print(job)
print(job.configuration)
if (job.jobDescription ==
SlideRunnerPlugin.JobDescription.QUIT_PLUGIN_THREAD):
# signal to exit this thread
quitSignal = True
continue
if (job.configuration['file']
== oldArchive) and (job.configuration['threshold']
== oldThres) and (job.slideFilename
== oldSlide):
continue
if not (os.path.exists(job.configuration['file'])):
continue
print('Performing label update')
self.sendAnnotationLabelUpdate()
oldArchive = job.configuration['file']
oldThres = job.configuration['threshold']
oldSlide = job.slideFilename
[foo, self.ext] = os.path.splitext(oldArchive)
self.ext = self.ext.upper()
self.annos = list()
if (self.ext == '.P'): # Pickled format - results for many slides
self.resultsArchive = pickle.load(open(oldArchive, 'rb'))
pname, fname = os.path.split(job.slideFilename)
fnamewithfolder = pname.split(os.sep)[-1] + os.sep + fname
if (oldFilename is not fname):
# process slide
if (fname not in self.resultsArchive) and (
fnamewithfolder not in self.resultsArchive):
self.setMessage('Slide ' + str(fname) +
' not found in results file.')
print('List is:', self.resultsArchive.keys())
continue
if (fnamewithfolder in self.resultsArchive):
fname = fnamewithfolder
oldFilename = fname
uniqueLabels = np.unique(
np.array(self.resultsArchive[fname])[:, 4])
self.annotationLabels = dict()
for key, label in enumerate(uniqueLabels):
self.annotationLabels[
label] = SlideRunnerPlugin.PluginAnnotationLabel(
key, 'Class %d' % label,
self.COLORS[key % len(self.COLORS)])
for idx in range(len(self.resultsArchive[fname])):
row = self.resultsArchive[fname][idx]
if (row[5] > job.configuration['threshold']):
myanno = annotations.rectangularAnnotation(
uid=idx,
x1=row[0],
x2=row[2],
y1=row[1],
y2=row[3],
text='%.2f' % row[5],
pluginAnnotationLabel=self.annotationLabels[
row[4]])
self.annos.append(myanno)
self.sendAnnotationLabelUpdate()
elif (self.ext == '.TXT'): # Assume MS Coco format
self.resultsArchive = np.loadtxt(
oldArchive,
dtype={
'names': ('label', 'confidence', 'x', 'y', 'w', 'h'),
'formats': ('U30', 'f4', 'i4', 'i4', 'i4', 'i4')
},
skiprows=0,
delimiter=' ')
uniqueLabels = np.unique(self.resultsArchive['label'])
self.annotationLabels = dict()
for key, label in enumerate(uniqueLabels):
self.annotationLabels[
label] = SlideRunnerPlugin.PluginAnnotationLabel(
key, label, self.COLORS[key % len(self.COLORS)])
self.sendAnnotationLabelUpdate()
for idx in range(len(self.resultsArchive)):
row = self.resultsArchive[idx]
if (row[5] > job.configuration['threshold']):
myanno = annotations.rectangularAnnotation(
uid=idx,
x1=row['x'],
y1=row['y'],
x2=row['x'] + row['w'],
y2=row['y'] + row['h'],
text='%.2f' % row['confidence'],
pluginAnnotationLabel=self.annotationLabels[
row['label']])
self.annos.append(myanno)
self.updateAnnotations()
self.setProgressBar(-1)
self.setMessage('found %d annotations.' % len(self.annos))
class Plugin(SlideRunnerPlugin.SlideRunnerPlugin):
version = 0.1
shortName = 'Object Detection Results'
inQueue = Queue()
outQueue = Queue()
initialOpacity = 1.0
updateTimer = 0.1
outputType = SlideRunnerPlugin.PluginOutputType.NO_OVERLAY
description = 'Show unpickled object detection results'
pluginType = SlideRunnerPlugin.PluginTypes.WHOLESLIDE_PLUGIN
configurationList = list((
SlideRunnerPlugin.FilePickerConfigurationEntry(uid='file',
name='Result file',
mask='*.p;;*.txt'),
SlideRunnerPlugin.PluginConfigurationEntry(uid='threshold',
name='Detection threshold',
initValue=0.75,
minValue=0.0,
maxValue=1.0),
))
COLORS = [[0, 128, 0, 255], [128, 0, 0, 255], [0, 0, 128, 255],
[128, 128, 0, 255], [0, 128, 128, 255], [128, 128, 128, 255]]
def __init__(self, statusQueue: Queue):
self.statusQueue = statusQueue
self.annotationLabels = {
'Detection':
SlideRunnerPlugin.PluginAnnotationLabel(0, 'Detection',
[0, 180, 0, 255]),
}
self.p = Thread(target=self.queueWorker, daemon=True)
self.p.start()
pass
def getAnnotationUpdatePolicy():
# This is important to tell SlideRunner that he needs to update for every change in position.
return SlideRunnerPlugin.AnnotationUpdatePolicy.UPDATE_ON_SLIDE_CHANGE
def queueWorker(self):
debugModule = False
quitSignal = False
oldFilename = ''
oldArchive = ''
oldSlide = ''
oldThres = -1
while not quitSignal:
job = SlideRunnerPlugin.pluginJob(self.inQueue.get())
print(job)
print(job.configuration)
if (job.jobDescription ==
SlideRunnerPlugin.JobDescription.QUIT_PLUGIN_THREAD):
# signal to exit this thread
quitSignal = True
continue
if (job.configuration['file']
== oldArchive) and (job.configuration['threshold']
== oldThres) and (job.slideFilename
== oldSlide):
continue
if not (os.path.exists(job.configuration['file'])):
continue
print('Performing label update')
self.sendAnnotationLabelUpdate()
oldArchive = job.configuration['file']
oldThres = job.configuration['threshold']
oldSlide = job.slideFilename
[foo, self.ext] = os.path.splitext(oldArchive)
self.ext = self.ext.upper()
self.annos = list()
if (self.ext == '.P'): # Pickled format - results for many slides
self.resultsArchive = pickle.load(open(oldArchive, 'rb'))
pname, fname = os.path.split(job.slideFilename)
fnamewithfolder = pname.split(os.sep)[-1] + os.sep + fname
if (oldFilename is not fname):
# process slide
if (fname not in self.resultsArchive) and (
fnamewithfolder not in self.resultsArchive):
self.setMessage('Slide ' + str(fname) +
' not found in results file.')
print('List is:', self.resultsArchive.keys())
continue
if (fnamewithfolder in self.resultsArchive):
fname = fnamewithfolder
oldFilename = fname
uniqueLabels = np.unique(
np.array(self.resultsArchive[fname])[:, 4])
self.annotationLabels = dict()
for key, label in enumerate(uniqueLabels):
self.annotationLabels[
label] = SlideRunnerPlugin.PluginAnnotationLabel(
key, 'Class %d' % label,
self.COLORS[key % len(self.COLORS)])
for idx in range(len(self.resultsArchive[fname])):
row = self.resultsArchive[fname][idx]
if (row[5] > job.configuration['threshold']):
myanno = annotations.rectangularAnnotation(
uid=idx,
x1=row[0],
x2=row[2],
y1=row[1],
y2=row[3],
text='%.2f' % row[5],
pluginAnnotationLabel=self.annotationLabels[
row[4]])
self.annos.append(myanno)
self.sendAnnotationLabelUpdate()
elif (self.ext == '.TXT'): # Assume MS Coco format
self.resultsArchive = np.loadtxt(
oldArchive,
dtype={
'names': ('label', 'confidence', 'x', 'y', 'w', 'h'),
'formats': ('U30', 'f4', 'i4', 'i4', 'i4', 'i4')
},
skiprows=0,
delimiter=' ')
uniqueLabels = np.unique(self.resultsArchive['label'])
self.annotationLabels = dict()
for key, label in enumerate(uniqueLabels):
self.annotationLabels[
label] = SlideRunnerPlugin.PluginAnnotationLabel(
key, label, self.COLORS[key % len(self.COLORS)])
self.sendAnnotationLabelUpdate()
for idx in range(len(self.resultsArchive)):
row = self.resultsArchive[idx]
if (row[5] > job.configuration['threshold']):
myanno = annotations.rectangularAnnotation(
uid=idx,
x1=row['x'],
y1=row['y'],
x2=row['x'] + row['w'],
y2=row['y'] + row['h'],
text='%.2f' % row['confidence'],
pluginAnnotationLabel=self.annotationLabels[
row['label']])
self.annos.append(myanno)
self.updateAnnotations()
self.setProgressBar(-1)
self.setMessage('found %d annotations.' % len(self.annos))
def getAnnotations(self):
return self.annos
def getAnnotationLabels(self):
# sending default annotation labels
return [self.annotationLabels[k] for k in self.annotationLabels.keys()]
def queueWorker(self):
oldwsi=None
quitSignal=False
sl=None
detected_offset = None
sl_main = None
while not quitSignal:
job = SlideRunnerPlugin.pluginJob(self.inQueue.get())
image = job.currentImage
mainWSI = job.slideFilename
if 'file' not in job.configuration:
continue
if (job.configuration['file'] != oldwsi) and (job.configuration['file']!='') and job.configuration['file']:
sl = openslide.open_slide(job.configuration['file'])
if (mainWSI):
sl_main = openslide.open_slide(mainWSI)
if (job.trigger is not None) and job.trigger.uid=='match':
print('Trigger: ',job.trigger)
self.setProgressBar(0)
self.setMessage('Calculating optimum offset')
tissue_detector = TissueDetector("LAB_Threshold", threshold=80) # option 1
matcher_parameters = MatcherParameters() # use the default parameters
matcher = WSI_Matcher(tissue_detector, matcher_parameters)
detected_offset = matcher.match(mainWSI, job.configuration['file'])
self.setProgressBar(-1)
updateConfig = list()
updateConfig.append(SlideRunnerPlugin.PluginConfigUpdateEntry(SlideRunnerPlugin.PluginConfigurationType.SLIDER_WITH_FLOAT_VALUE, uid='xoffset', value=detected_offset[0]))
updateConfig.append(SlideRunnerPlugin.PluginConfigUpdateEntry(SlideRunnerPlugin.PluginConfigurationType.SLIDER_WITH_FLOAT_VALUE, uid='yoffset', value=detected_offset[1]))
self.updateConfiguration(SlideRunnerPlugin.PluginConfigUpdate(updateConfig))
if (sl) and (sl_main):
self.setProgressBar(0)
print('Reading from: ',job)
zoomValue=job.coordinates[3]/job.currentImage.shape[0]
print('Zoom value: ',zoomValue)
act_level = np.argmin(np.abs(np.asarray(sl.level_downsamples)-zoomValue))
closest_ds = sl_main.level_downsamples[np.argmin(np.abs(np.asarray(sl_main.level_downsamples)-zoomValue))]
offset = (job.configuration['xoffset'],job.configuration['yoffset'])
if (detected_offset is not None):
offset=detected_offset
offset_scaled = [int(x/closest_ds) for x in offset]
print('Scaled offset is: ',offset_scaled)
imgarea_w=job.coordinates[2:4]
size_im = (int(imgarea_w[0]/closest_ds), int(imgarea_w[1]/closest_ds))
print('Image size: ',size_im)
location = [int(x+y) for x,y in zip(job.coordinates[0:2],offset)]
print('Location (original):',job.coordinates[0:2])
print('Location (offset): ',location)
img = sl.read_region(location=location, level=act_level, size=size_im)
img = np.array(img.resize((job.currentImage.shape[1],job.currentImage.shape[0] )))
self.returnImage(img, job.procId)
self.setMessage('Align done.')
self.setProgressBar(-1)
if (job.jobDescription == SlideRunnerPlugin.JobDescription.QUIT_PLUGIN_THREAD):
# signal to exit this thread
quitSignal=True
continue
print('OTSU plugin: received 1 image from queue')
class Plugin(SlideRunnerPlugin.SlideRunnerPlugin):
version = 0.0
shortName = 'High Power Field Visualization'
inQueue = Queue()
outQueue = Queue()
description = 'Display size of 1 HPF'
pluginType = SlideRunnerPlugin.PluginTypes.WHOLESLIDE_PLUGIN
outputType = SlideRunnerPlugin.PluginOutputType.NO_OVERLAY
modelInitialized = False
updateTimer = 0.1
slideFilename = None
annos = list()
configurationList = list((
SlideRunnerPlugin.PluginConfigurationEntry(
uid=0,
name='Re-center HPF',
ctype=SlideRunnerPlugin.PluginConfigurationType.PUSHBUTTON),
SlideRunnerPlugin.PluginConfigurationEntry(uid=1,
name='Number of HPFs',
initValue=1.00,
minValue=1.0,
maxValue=10.0),
SlideRunnerPlugin.PluginConfigurationEntry(uid=2,
name='Size of HPF (mm2)',
initValue=0.237,
minValue=0.20,
maxValue=0.3),
)) #0.237
def __init__(self, statusQueue: Queue):
self.statusQueue = statusQueue
self.p = Thread(target=self.queueWorker, daemon=True)
self.p.start()
pass
def getAnnotationUpdatePolicy():
# This is important to tell SlideRunner that he needs to update for every change in position.
return SlideRunnerPlugin.AnnotationUpdatePolicy.UPDATE_ON_SLIDE_CHANGE
def queueWorker(self):
quitSignal = False
oldSlide = ''
while not quitSignal:
job = SlideRunnerPlugin.pluginJob(self.inQueue.get())
filename = job
if (job.jobDescription ==
SlideRunnerPlugin.JobDescription.QUIT_PLUGIN_THREAD):
# signal to exit this thread
quitSignal = True
continue
if (job.slideFilename != oldSlide) or (job.trigger is not None):
self.processWholeSlide(job)
oldSlide = job.slideFilename
else:
print('Trigger:', job.trigger)
def getAnnotations(self):
return self.annos
def processWholeSlide(self, job: SlideRunnerPlugin.pluginJob):
filename = job.slideFilename
self.slide = openslide.open_slide(filename)
# 1 HPF = 0.237 mm^2
A = job.configuration[2] # mm^2
W_hpf_microns = np.sqrt(A * 4 / 3) * 1000 # in microns
H_hpf_microns = np.sqrt(A * 3 / 4) * 1000 # in microns
micronsPerPixel = self.slide.properties[openslide.PROPERTY_NAME_MPP_X]
W_hpf = int(W_hpf_microns / float(micronsPerPixel)) * np.sqrt(
float(int(job.configuration[1])))
H_hpf = int(H_hpf_microns / float(micronsPerPixel)) * np.sqrt(
float(int(job.configuration[1])))
center = (int((job.coordinates[0] + 0.5 * job.coordinates[2])),
int((job.coordinates[1] + 0.5 * job.coordinates[3])))
self.annos = list()
if (int(job.configuration[1]) == 1):
myanno = annotations.rectangularAnnotation(0,
center[0] - W_hpf / 2,
center[1] - H_hpf / 2,
center[0] + W_hpf / 2,
center[1] + H_hpf / 2,
'High-Power Field')
else:
myanno = annotations.rectangularAnnotation(
0, center[0] - W_hpf / 2, center[1] - H_hpf / 2,
center[0] + W_hpf / 2, center[1] + H_hpf / 2,
'%d High-Power Fields' % int(job.configuration[1]))
self.annos.append(myanno)
self.updateAnnotations()
class Plugin(SlideRunnerPlugin.SlideRunnerPlugin):
version = 0.1
shortName = 'Secondary database visualization'
inQueue = Queue()
outQueue = Queue()
initialOpacity = 1.0
updateTimer = 0.1
outputType = SlideRunnerPlugin.PluginOutputType.RGB_IMAGE
description = 'Visualize secondary SlideRunner database'
pluginType = SlideRunnerPlugin.PluginTypes.WHOLESLIDE_PLUGIN
configurationList = list(
(SlideRunnerPlugin.FilePickerConfigurationEntry(uid='file',
name='Database file',
mask='*.sqlite'), ))
COLORS = [[0, 128, 0, 255], [128, 0, 0, 255], [0, 0, 128, 255],
[128, 128, 0, 255], [0, 128, 128, 255], [128, 128, 128, 255]]
def __init__(self, statusQueue: Queue):
self.statusQueue = statusQueue
self.annotationLabels = {}
self.secondaryDB = Database()
self.p = Thread(target=self.queueWorker, daemon=True)
self.p.start()
pass
def getAnnotationUpdatePolicy():
# This is important to tell SlideRunner that he needs to update for every change in position.
return SlideRunnerPlugin.AnnotationUpdatePolicy.UPDATE_ON_SLIDE_CHANGE
def queueWorker(self):
debugModule = False
quitSignal = False
oldFilename = ''
oldArchive = ''
oldSlide = ''
oldThres = -1
while not quitSignal:
job = SlideRunnerPlugin.pluginJob(self.inQueue.get())
print(job)
if (job.jobDescription ==
SlideRunnerPlugin.JobDescription.QUIT_PLUGIN_THREAD):
# signal to exit this thread
quitSignal = True
continue
if (job.configuration['file'] == oldArchive) and (job.slideFilename
== oldSlide):
continue
if not (os.path.exists(job.configuration['file'])):
continue
self.sendAnnotationLabelUpdate()
oldArchive = job.configuration['file']
oldSlide = job.slideFilename
self.secondaryDB.open(oldArchive)
self.annos = list()
self.annotationLabels = dict()
for key, (label, annoId,
col) in enumerate(self.secondaryDB.getAllClasses()):
self.annotationLabels[
annoId] = SlideRunnerPlugin.PluginAnnotationLabel(
0, '%s' % label, [*hex_to_rgb(col), 0])
pname, fname = os.path.split(job.slideFilename)
self.slideUID = self.secondaryDB.findSlideWithFilename(
fname, pname)
self.secondaryDB.loadIntoMemory(self.slideUID)
self.annos = list()
for annoId in self.secondaryDB.annotations.keys():
anno = self.secondaryDB.annotations[annoId]
anno.pluginAnnotationLabel = self.annotationLabels[
anno.agreedClass]
self.annos.append(anno)
self.sendAnnotationLabelUpdate()
self.updateAnnotations()
self.setProgressBar(-1)
self.setMessage('found %d annotations.' % len(self.annos))
def getAnnotations(self):
return self.annos
def getAnnotationLabels(self):
# sending default annotation labels
return [self.annotationLabels[k] for k in self.annotationLabels.keys()]
class Plugin(SlideRunnerPlugin.SlideRunnerPlugin):
version = 0.1
shortName = 'Normalize (Macenko)'
inQueue = Queue()
outQueue = Queue()
initialOpacity = 1.0
updateTimer = 0.5
outputType = SlideRunnerPlugin.PluginOutputType.RGB_IMAGE
description = 'H&E Image normalization (Method by Macenko)'
pluginType = SlideRunnerPlugin.PluginTypes.IMAGE_PLUGIN
configurationList = list(
(SlideRunnerPlugin.ComboboxPluginConfigurationEntry(
uid='mode',
name='Mode',
options=['show H&E', 'only E', 'only H'],
selected_value=0), ))
def __init__(self, statusQueue: Queue):
self.statusQueue = statusQueue
self.p = Thread(target=self.queueWorker, daemon=True)
self.p.start()
pass
def queueWorker(self):
quitSignal = False
while not quitSignal:
job = SlideRunnerPlugin.pluginJob(self.inQueue.get())
image = job.currentImage
if (job.jobDescription ==
SlideRunnerPlugin.JobDescription.QUIT_PLUGIN_THREAD):
# signal to exit this thread
quitSignal = True
continue
print('Macenko norm plugin: received 1 image from queue')
self.setProgressBar(0)
print(job)
if (job.annotations is not None) and len(job.annotations) > 0:
if (job.annotations[0].annotationType ==
annotations.AnnotationType.AREA):
print('Found an area annotation - great!')
minC = job.annotations[0].minCoordinates()
maxC = job.annotations[0].maxCoordinates()
scaleX = (job.coordinates[2]) / job.currentImage.shape[1]
scaleY = (job.coordinates[3]) / job.currentImage.shape[0]
minC = np.array(
(max(0, int((minC.x - job.coordinates[0]) / scaleX)),
max(0, int((minC.y - job.coordinates[1]) / scaleY))))
maxC = np.array(
(min(job.currentImage.shape[1],
int((maxC.x - job.coordinates[0]) / scaleX)),
min(job.currentImage.shape[0],
int((maxC.y - job.coordinates[1]) / scaleY))))
rgb = np.copy(image[:, :, 0:3])
rgb = normalize(rgb,
np.reshape(
rgb[minC[1]:maxC[1],
minC[0]:maxC[0], :], (-1, 3)),
job=job)
else:
rgb = np.copy(image[:, :, 0:3])
rgb = normalize(rgb, job=job)
print('Stats: ', np.max(np.float32(rgb)), np.min(np.float32(rgb)),
np.mean(np.float32(rgb)),
np.float32(rgb).shape)
self.returnImage(np.float32(rgb), job.procId)
self.setMessage('Macenko normalization: done.')
self.setProgressBar(-1)
