def promoter_assemblies_build_more_than_384():
utr_linkers = [
"UTR1-RBS1",
"UTR1-RBS2",
"UTR1-RBS3",
"UTR2-RBS1",
"UTR2-RBS2",
"UTR2-RBS3",
"UTR3-RBS1",
"UTR3-RBS2",
"UTR3-RBS3",
]
promoter_assemblies = []
for utr_linker in utr_linkers:
promoter_assemblies += [
bsb.BasicAssembly(
f"promoter_construct_{ind}_{utr_linker}",
bsb.BASIC_SEVA_PARTS["v0.1"]["26"],
bsb.BASIC_BIOLEGIO_LINKERS["v0.1"]["LMP"],
promoter,
bsb.BASIC_BIOLEGIO_LINKERS["v0.1"][utr_linker],
bsb.BASIC_CDS_PARTS["v0.1"]["sfGFP"],
bsb.BASIC_BIOLEGIO_LINKERS["v0.1"]["LMS"],
) for ind, promoter in enumerate(
bsb.BASIC_PROMOTER_PARTS["v0.1"].values())
]
return bsb.BasicBuild(*promoter_assemblies)
def test_basic_build_indetical_ids(five_part_assembly):
from basicsynbio.cam import BuildException
with pytest.raises(
BuildException,
match=f"ID '{five_part_assembly.id}' has been assigned to 2 BasicAssembly instance/s. All assemblies of a build should have a unique 'id' attribute.",
):
bsb.BasicBuild(five_part_assembly, five_part_assembly)
def build_json():
promoter_assemblies = (bsb.BasicAssembly(
f"promoter_construct_{ind}",
bsb.BASIC_SEVA_PARTS["v0.1"]["26"],
bsb.BASIC_BIOLEGIO_LINKERS["v0.1"]["LMP"],
promoter,
bsb.BASIC_BIOLEGIO_LINKERS["v0.1"]["UTR1-RBS2"],
bsb.BASIC_CDS_PARTS["v0.1"]["sfGFP"],
bsb.BASIC_BIOLEGIO_LINKERS["v0.1"]["LMS"],
) for ind, promoter in enumerate(bsb.BASIC_PROMOTER_PARTS["v0.1"].values())
)
build = bsb.BasicBuild(*promoter_assemblies)
return build
def small_build_example():
return bsb.BasicBuild(
bsb.BasicAssembly(
"First_Assembly_With_18",
bsb.BASIC_SEVA_PARTS["v0.1"]["18"],
bsb.BASIC_BIOLEGIO_LINKERS["v0.1"]["LMP"],
bsb.BASIC_CDS_PARTS["v0.1"]["sfGFP"],
bsb.BASIC_BIOLEGIO_LINKERS["v0.1"]["LMS"],
),
bsb.BasicAssembly(
"Second_Assembly_With_26",
bsb.BASIC_SEVA_PARTS["v0.1"]["26"],
bsb.BASIC_BIOLEGIO_LINKERS["v0.1"]["LMP"],
bsb.BASIC_CDS_PARTS["v0.1"]["sfGFP"],
bsb.BASIC_BIOLEGIO_LINKERS["v0.1"]["LMS"],
),
)