예제 #1
0
def main(args):
    '''Run CellProfiler

    args - command-line arguments, e.g. sys.argv
    '''
    if any([arg.startswith('--work-announce') for arg in args]):
        #
        # Go headless ASAP
        #
        import cellprofiler.preferences as cpprefs
        cpprefs.set_headless()
        import cellprofiler.analysis_worker
        cellprofiler.analysis_worker.main()
        sys.exit(0)

    options, args = parse_args(args)
    set_log_level(options)

    if not hasattr(sys, "frozen") and options.code_statistics:
        print_code_statistics()
        return

    if options.print_groups_file is not None:
        print_groups(options.print_groups_file)
        return

    if options.batch_commands_file is not None:
        get_batch_commands(options.batch_commands_file)
        return

    if options.run_ilastik:
        run_ilastik()
        return

    # necessary to prevent matplotlib trying to use Tkinter as its backend.
    # has to be done before CellProfilerApp is imported
    from matplotlib import use as mpluse
    mpluse('WXAgg')

    if (not hasattr(sys, 'frozen')) and options.fetch_external_dependencies:
        import external_dependencies
        external_dependencies.fetch_external_dependencies(
            options.overwrite_external_dependencies)

    if (not hasattr(sys, 'frozen')) and options.build_extensions:
        build_extensions()
        if options.build_and_exit:
            return

    if options.output_html:
        from cellprofiler.gui.html.manual import generate_html
        webpage_path = options.output_directory if options.output_directory else None
        generate_html(webpage_path)
        return
    if options.print_measurements:
        print_measurements(options)
        return

    try:
        if options.show_gui:
            import wx
            wx.Log.EnableLogging(False)
            from cellprofiler.cellprofilerapp import CellProfilerApp
            show_splashbox = (options.pipeline_filename is None
                              and options.workspace_filename is None
                              and (not options.new_workspace)
                              and options.show_splashbox)
            if options.workspace_filename:
                workspace_path = os.path.expanduser(options.workspace_filename)
            elif options.new_workspace:
                workspace_path = False
            else:
                workspace_path = None
            App = CellProfilerApp(
                0,
                check_for_new_version=(options.pipeline_filename is None),
                show_splashbox=show_splashbox,
                workspace_path=workspace_path)

        #
        # Important to go headless ASAP
        #
        # cellprofiler.preferences can't be imported before we have a chance
        # to initialize the wx app.
        #
        import cellprofiler.preferences as cpprefs
        if not options.show_gui:
            cpprefs.set_headless()
            # What's there to do but run if you're running headless?
            # Might want to change later if there's some headless setup
            options.run_pipeline = True

        if options.plugins_directory is not None:
            cpprefs.set_plugin_directory(options.plugins_directory)
        if options.ij_plugins_directory is not None:
            cpprefs.set_ij_plugin_directory(options.ij_plugins_directory)
        if options.temp_dir is not None:
            cpprefs.set_temporary_directory(options.temp_dir)
        if options.data_file is not None:
            cpprefs.set_data_file(os.path.abspath(options.data_file))
        if options.image_set_file is not None:
            cpprefs.set_image_set_file(options.image_set_file, False)

        from cellprofiler.utilities.version import version_string, version_number
        logging.root.info("Version: %s / %d" %
                          (version_string, version_number))

        if options.run_pipeline and not options.pipeline_filename:
            raise ValueError("You must specify a pipeline filename to run")

        if options.output_directory:
            cpprefs.set_default_output_directory(options.output_directory)

        if options.image_directory:
            cpprefs.set_default_image_directory(options.image_directory)

        if options.show_gui:
            import cellprofiler.gui.cpframe as cpgframe
            if options.pipeline_filename:
                pipeline_path = os.path.expanduser(options.pipeline_filename)
                try:
                    App.frame.pipeline.load(pipeline_path)
                    if options.run_pipeline:
                        App.frame.Command(cpgframe.ID_FILE_ANALYZE_IMAGES)
                except:
                    import wx
                    wx.MessageBox(
                        'CellProfiler was unable to load the pipeline file, "%s"'
                        % options.pipeline_filename,
                        "Error loading pipeline",
                        style=wx.OK | wx.ICON_ERROR)
                    logging.root.error("Unable to load pipeline",
                                       exc_info=True)
            App.MainLoop()
            return

        elif options.run_pipeline:
            run_pipeline_headless(options, args)
    except Exception, e:
        logging.root.fatal("Uncaught exception in CellProfiler.py",
                           exc_info=True)
        raise
예제 #2
0
def main(args):
    '''Run CellProfiler

    args - command-line arguments, e.g. sys.argv
    '''
    if any([arg.startswith('--work-announce') for arg in args]):
        #
        # Go headless ASAP
        #
        import cellprofiler.preferences as cpprefs
        cpprefs.set_headless()
        for i, arg in enumerate(args):
            if arg == "--ij-plugins-directory" and len(args) > i+1:
                cpprefs.set_ij_plugin_directory(args[i+1])
                break
        import cellprofiler.analysis_worker
        cellprofiler.analysis_worker.aw_parse_args()
        cellprofiler.analysis_worker.main()
        sys.exit(0)
        
    options, args = parse_args(args)
    if options.jvm_heap_size != None:
        from cellprofiler.preferences import set_jvm_heap_mb
        set_jvm_heap_mb(options.jvm_heap_size, False)
    set_log_level(options)
    
    if not hasattr(sys, "frozen") and options.code_statistics:
        print_code_statistics()
        return
    
    if options.print_groups_file is not None:
        print_groups(options.print_groups_file)
        return
    
    if options.batch_commands_file is not None:
        get_batch_commands(options.batch_commands_file)
        return
        
    if options.run_ilastik:
        run_ilastik()
        return
    
    if options.add_message_for_user:
        if len(args) != 3:
            sys.stderr.write("Usage: (for add_message-for-user)\n")
            sys.stderr.write("CellProfiler --add-message-for-user <caption> <message> <pipeline-or-project>\n")
            sys.stderr.write("where:\n")
            sys.stderr.write("    <caption> - the message box caption\n")
            sys.stderr.write("    <message> - the message displayed inside the message box\n")
            sys.stderr.write("    <pipeline-or-project> - the path to the pipeline or project file to modify\n")
            return
        caption = args[0]
        message = args[1]
        path = args[2]
        
        import h5py
        using_hdf5 = h5py.is_hdf5(path)
        if using_hdf5:
            import cellprofiler.measurements as cpmeas
            m = cpmeas.Measurements(
                filename = path, mode="r+")
            pipeline_text = m[cpmeas.EXPERIMENT, "Pipeline_Pipeline"]
        else:
            with open(path, "r") as fd:
                pipeline_text = fd.read()
        header, body = pipeline_text.split("\n\n", 1)
        pipeline_text = header + \
            ("\nMessageForUser:%s|%s\n\n" % (caption, message)) + body
        if using_hdf5:
            m[cpmeas.EXPERIMENT, "Pipeline_Pipeline"] = pipeline_text
            m.close()
        else:
            with open(path, "w") as fd:
                fd.write(pipeline_text)
        print "Message added to %s" % path
        return
    
    # necessary to prevent matplotlib trying to use Tkinter as its backend.
    # has to be done before CellProfilerApp is imported
    from matplotlib import use as mpluse
    mpluse('WXAgg')
    
    if (not hasattr(sys, 'frozen')) and options.fetch_external_dependencies:
        import external_dependencies
        external_dependencies.fetch_external_dependencies(options.overwrite_external_dependencies)
    
    if (not hasattr(sys, 'frozen')) and options.build_extensions:
        build_extensions()
        if options.build_and_exit:
            return
    
    if options.output_html:
        from cellprofiler.gui.html.manual import generate_html
        webpage_path = options.output_directory if options.output_directory else None
        generate_html(webpage_path)
        return
    if options.print_measurements:
        print_measurements(options)
        return
    if options.omero_credentials is not None:
        set_omero_credentials_from_string(options.omero_credentials)
    try:
        if options.show_gui:
            import wx
            wx.Log.EnableLogging(False)
            from cellprofiler.cellprofilerapp import CellProfilerApp
            from cellprofiler.workspace import is_workspace_file
            show_splashbox = (options.pipeline_filename is None and
                              (not options.new_project) and
                              options.show_splashbox)
            
            if options.pipeline_filename:
                if is_workspace_file(options.pipeline_filename):
                    workspace_path = os.path.expanduser(options.pipeline_filename)
                    pipeline_path = None
                else:
                    pipeline_path = os.path.expanduser(options.pipeline_filename)
                    workspace_path = None
            elif options.new_project:
                workspace_path = False
                pipeline_path = None
            else:
                workspace_path = None
                pipeline_path = None
            App = CellProfilerApp(
                0, 
                check_for_new_version = (options.pipeline_filename is None),
                show_splashbox = show_splashbox,
                workspace_path = workspace_path,
                pipeline_path = pipeline_path)
    
        #
        # Important to go headless ASAP
        #
        # cellprofiler.preferences can't be imported before we have a chance
        # to initialize the wx app.
        #
        import cellprofiler.preferences as cpprefs
        if not options.show_gui:
            cpprefs.set_headless()
            # What's there to do but run if you're running headless?
            # Might want to change later if there's some headless setup 
            options.run_pipeline = True
    
            
        if options.plugins_directory is not None:
            cpprefs.set_plugin_directory(options.plugins_directory)
        if options.ij_plugins_directory is not None:
            cpprefs.set_ij_plugin_directory(options.ij_plugins_directory)
        if options.temp_dir is not None:
            if not os.path.exists(options.temp_dir):
                os.makedirs(options.temp_dir)
            cpprefs.set_temporary_directory(options.temp_dir)
        if options.data_file is not None:
            cpprefs.set_data_file(os.path.abspath(options.data_file))
        if options.image_set_file is not None:
            cpprefs.set_image_set_file(options.image_set_file, False)
            
        from cellprofiler.utilities.version import version_string, version_number
        logging.root.info("Version: %s / %d" % (version_string, version_number))
    
        if options.run_pipeline and not options.pipeline_filename:
            raise ValueError("You must specify a pipeline filename to run")
    
        if options.output_directory:
            if not os.path.exists(options.output_directory):
                os.makedirs(options.output_directory)
            cpprefs.set_default_output_directory(options.output_directory)
        
        if options.image_directory:
            cpprefs.set_default_image_directory(options.image_directory)
    
        if options.show_gui:
            if options.run_pipeline:
                App.frame.pipeline_controller.do_analyze_images()
            App.MainLoop()
            return
        
        elif options.run_pipeline:
            run_pipeline_headless(options, args)
    except Exception, e:
        logging.root.fatal("Uncaught exception in CellProfiler.py", exc_info=True)
        raise
예제 #3
0
    options.show_gui = False
    options.run_pipeline = True
    assert options.groups == None, "groups not supported in distributed processing, yet"
    try:
        worker_timeout = int(options.worker_timeout)
    except ValueError:
        logging.root.fatal("Can't convert timeout value '%s' to an integer.",
                           options.worker_timeout)
        sys.exit(0)

if options.show_gui and not options.output_html:
    import wx
    wx.Log.EnableLogging(False)
    from cellprofiler.cellprofilerapp import CellProfilerApp
    App = CellProfilerApp(
        0,
        check_for_new_version=(options.pipeline_filename is None),
        show_splashbox=(options.pipeline_filename is None))
    # ... loading a pipeline from the filename can bring up a modal
    # dialog, which causes a crash on Mac if the splashbox is open or
    # a second modal dialog is opened.

try:
    #
    # Important to go headless ASAP
    #
    import cellprofiler.preferences as cpprefs
    if (not options.show_gui) or options.output_html:
        cpprefs.set_headless()
        # What's there to do but run if you're running headless?
        # Might want to change later if there's some headless setup
        if (not options.output_html) and (not options.print_measurements):