def posterior_decoding():
"""Solution code below..."""
#print "Enter the name of the HMM file:"
#hmm_file = raw_input().strip()
hmm_file = "HMMmethanococcus.txt"
#print "Enter the name of the input file:"
#sys.stdout.flush()
#input_file = raw_input().strip()
input_file = "bacterial.genome.fasta"
f_in_file = open(input_file)
f_hmm_file = open(hmm_file)
if f_in_file is None:
sys.exit("Can't open HMM file: " + hmm_file)
if f_hmm_file is None:
sys.exit("Can't open file: " + input_file)
# read the state names
states = f_hmm_file.readline().split()
# read the initial probabilities
probs = f_hmm_file.readline().split()
initial_probs = [float(prob) for prob in probs]
# read the transition matrix
transitions = [None for _ in range(len(states))]
for i in range(0, len(states)):
matrix_row_arry = f_hmm_file.readline().split()
matrix_row = [float(trans_prob) for trans_prob in matrix_row_arry]
transitions[i] = matrix_row
# read the emitted symbols
emitted_symbols = f_hmm_file.readline().split()
# read the emission probability matrix
emit_probs = [None for _ in range(len(states))]
for i in range(0, len(states)):
matrix_row_arry = f_hmm_file.readline().split()
matrix_row = [float(emit_prob) for emit_prob in matrix_row_arry]
emit_probs[i] = matrix_row
f_hmm_file.close()
seq_dict = get_fasta_dict(input_file)
emit_str = seq_dict.values()[0] #there's only 1
test = len(emit_str)
print "Done reading sequence of length " , str(test)
print "\n.... Done reading sequence of length " + str(test)
#initializing the forward, backward and posterior 2D matrices
forward = [[float(0) for _ in range(len(states))] for _ in range(len(emit_str))]
backward = [[float(0) for _ in range(len(states))] for _ in range(len(emit_str))]
posterior = [[float(0) for _ in range(len(states))] for _ in range(len(emit_str))]
# Run the forward algorithm
run_forward(states, initial_probs, transitions, emitted_symbols, emit_probs, emit_str, forward)
# Run the backward algorithm
run_backward(states, initial_probs, transitions, emitted_symbols, emit_probs, emit_str, backward)
# Calculate the posterior probabilities
for i in range(0, len(emit_str)):
# Did not normalize the probabilities (i.e., did not divide by P(X)),
# because we will only use these probability to compare
# posterior[i][0] versus posterior[i][1].
for k in range(0, len(states)):
posterior[i][k] = forward[i][k] + backward[i][k]
# Print the decoded results
best_path = ""
for probabilities in posterior:
if probabilities[0] > probabilities[1]:
best_path += "0"
else:
best_path += "1"
print "The best path is " + best_path
print
print "Start Stop State"
state0 = True
start = 0
end = 0
number_regions = 0
temporary_list = []
for i in range(len(best_path)):
if (best_path[i] == "1" and state0 == True):
temporary_tuple = (start,i,"1",i-start)
print start, "\t", i, "\t", "1"
temporary_list.append(temporary_tuple)
start = i
state0 = False
if (best_path[i] == "0" and state0 == False):
state = "2"
temporary_tuple = (start,i,"2",i-start)
print start, "\t", i, "\t", state
temporary_list.append(temporary_tuple)
start = i
state0 = True
number_regions += 1
print "There are", number_regions, "structural RNA regions"
def posterior_decoding(input_file, f_hmm_file):
# read the state names and number
states = f_hmm_file.readline().split()
K = len(states)
# read the initial probabilities
probs = f_hmm_file.readline().split()
initial_probs = [float(prob) for prob in probs]
# read the transition matrix
transitions = [None for _ in range(K)]
for i in range(K):
matrix_row_arry = f_hmm_file.readline().split()
matrix_row = [float(trans_prob) for trans_prob in matrix_row_arry]
transitions[i] = matrix_row
# read the emission symbols
emission_symbols = f_hmm_file.readline().split()
# read the emission probability matrix
emit_probs = [None for _ in range(K)]
for i in range(K):
matrix_row_arry = f_hmm_file.readline().split()
matrix_row = [float(emit_prob) for emit_prob in matrix_row_arry]
emit_probs[i] = matrix_row
f_hmm_file.close()
seq_dict = get_fasta_dict(input_file)
emit_str = seq_dict.values()[0] # there's only 1
print "Done reading sequence of length ", len(emit_str)
for a in range(len(emit_probs)):
for c in range(len(transitions[0])):
transitions[a][c] = log(transitions[a][c])
for b in range(len(emit_probs[0])):
emit_probs[a][b] = log(emit_probs[a][b])
# Run the forward algorithm
forward = run_forward(states, initial_probs, transitions, emission_symbols,
emit_probs, emit_str)
# Run the backward algorithm
backward = run_backward(states, initial_probs, transitions,
emission_symbols, emit_probs, emit_str)
# Calculate the posterior probabilities
# Initializing the posterior 2D matrices
posterior = [[float(0) for _ in range(K)] for _ in range(len(emit_str))]
for i in range(len(emit_str)):
# Did not normalize the probabilities (i.e., did not divide by P(X)),
# because we will only use these probabilities to compare
# posterior[i][0] versus posterior[i][1]
for k in range(K):
posterior[i][k] = forward[i][k] + backward[i][k]
# Print out the decoded results
print "start\tstop\tstate"
total_count = 0
state2_count = 0
curr_state = 0
start = 0
for i in range(len(posterior)):
max = float('-inf')
max_state = 0
for k in range(K):
if (posterior[i][k] > max):
max = posterior[i][k]
max_state = k
if (max_state != curr_state or i == len(posterior) - 1):
total_count += 1
if curr_state == 1:
state2_count += 1
if i == len(posterior) - 1:
print start + 1, "\t", i + 1, "\tstate", curr_state + 1
else:
print start + 1, "\t", i, "\tstate", curr_state + 1
start = i
curr_state = max_state
print "Total number of regions reported:", total_count
print "Total number of state 2 regions:", state2_count
def posterior_decoding(input_file, hmm_file):
"""
Calculate the posterior decoding and return the decoded segments.
input_file (str): path to input fasta file
hmm_file (str): path to HMM file
Returns:
A list of dictionaries of segments in each state. An example output may
look like:
[
{‘start’: 0, ‘end’: 12, ‘state’: ‘state2’},
{‘start’: 13, ‘end’: 20, ‘state’: ‘state1’},
...
]
"""
# Read in the input files
f_in_file = open(input_file)
f_hmm_file = open(hmm_file)
if f_in_file is None: sys.exit("Can't open HMM file: " + hmm_file)
if f_hmm_file is None: sys.exit("Can't open file: " + input_file)
# read the state names and number
states = f_hmm_file.readline().split()
K = len(states)
# read the initial probabilities
probs = f_hmm_file.readline().split()
initial_probs = [float(prob) for prob in probs]
# read the transition matrix
transitions = [None for _ in range(K)]
for i in range(K):
matrix_row_arry = f_hmm_file.readline().split()
matrix_row = [float(trans_prob) for trans_prob in matrix_row_arry]
transitions[i] = matrix_row
# read the emission symbols
emission_symbols = f_hmm_file.readline().split()
# read the emission probability matrix
emit_probs = [None for _ in range(K)]
for i in range(K):
matrix_row_arry = f_hmm_file.readline().split()
matrix_row = [float(emit_prob) for emit_prob in matrix_row_arry]
emit_probs[i] = matrix_row
f_hmm_file.close()
seq_dict = get_fasta_dict(input_file)
emit_str = list(seq_dict.values())[0] # there's only 1
print(("Done reading sequence of length ", len(emit_str)))
# Run the forward algorithm
forward = run_forward(states, initial_probs, transitions,
emission_symbols, emit_probs, emit_str)
# Run the backward algorithm
backward = run_backward(states, initial_probs,
transitions, emission_symbols, emit_probs,
emit_str)
# Calculate the posterior probabilities
# Initializing the posterior 2D matrices
posterior = [[float(0) for _ in range(K)] for _ in range(len(emit_str))]
for i in range(len(emit_str)):
# Did not normalize the probabilities (i.e., did not divide by P(X)),
# because we will only use these probabilities to compare
# posterior[i][0] versus posterior[i][1]
for k in range(K):
posterior[i][k] = forward[i][k] + backward[i][k]
# Create the list of decoded segments to return
"""YOUR CODE HERE"""
return []
def posterior_decoding(f_in_file, f_hmm_file):
# read the state names and number
states = f_hmm_file.readline().split()
K = len(states)
# read the initial probabilities
probs = f_hmm_file.readline().split()
initial_probs = [float(prob) for prob in probs]
# read the transition matrix
transitions = [None for _ in range(K)]
for i in range(K):
matrix_row_arry = f_hmm_file.readline().split()
matrix_row = [float(trans_prob) for trans_prob in matrix_row_arry]
transitions[i] = matrix_row
# read the emission symbols
emission_symbols = f_hmm_file.readline().split()
# read the emission probability matrix
emit_probs = [None for _ in range(K)]
for i in range(K):
matrix_row_arry = f_hmm_file.readline().split()
matrix_row = [float(emit_prob) for emit_prob in matrix_row_arry]
emit_probs[i] = matrix_row
f_hmm_file.close()
seq_dict = get_fasta_dict(input_file)
emit_str = seq_dict.values()[0] # there's only 1
print "Done reading sequence of length ", len(emit_str)
# Run the forward algorithm
forward = run_forward(states, initial_probs, transitions, emission_symbols,
emit_probs, emit_str)
# Run the backward algorithm
backward = run_backward(states, initial_probs, transitions,
emission_symbols, emit_probs, emit_str)
# Calculate the posterior probabilities
# Initializing the posterior 2D matrices
posterior = [[float(0) for _ in range(K)] for _ in range(len(emit_str))]
for i in range(len(emit_str)):
# Did not normalize the probabilities (i.e., did not divide by P(X)),
# because we will only use these probabilities to compare
# posterior[i][0] versus posterior[i][1]
for k in range(K):
posterior[i][k] = forward[i][k] + backward[i][k]
# Print out the decoded results
# for post in posterior:
# max_p
# for k in range(K):
trace = []
for post in posterior:
max_post = float("-inf")
max_state = 0
for k in range(K):
if post[k] > max_post:
max_post = post[k]
max_state = k
trace.append(max_state)
# print trace[1627030:1627050]
# print trace [1627041-1:1627042+2]
# display_trix(trace)
catch_top_ten(trace)
catch_bottom_ten(trace)
def posterior_decoding():
hmm_file = raw_input("Enter the name of the HMM file:").strip()
sys.stdout.flush()
input_file = raw_input("Enter the name of the input file:").strip()
f_in_file = open(input_file)
f_hmm_file = open(hmm_file)
if f_in_file is None:
sys.exit("Can't open HMM file: " + hmm_file)
if f_hmm_file is None:
sys.exit("Can't open file: " + input_file)
# read the state names and number
states = f_hmm_file.readline().split()
K = len(states)
# read the initial probabilities
probs = f_hmm_file.readline().split()
initial_probs = [log(float(prob)) for prob in probs]
# read the transition matrix
transitions = [None for _ in range(K)]
for i in range(K):
matrix_row_arry = f_hmm_file.readline().split()
matrix_row = [log(float(trans_prob)) for trans_prob in matrix_row_arry]
transitions[i] = matrix_row
# read the emitted symbols
emitted_symbols = f_hmm_file.readline().split()
# read the emission probability matrix
emit_probs = [None for _ in range(K)]
for i in range(K):
matrix_row_arry = f_hmm_file.readline().split()
matrix_row = [log(float(emit_prob)) for emit_prob in matrix_row_arry]
emit_probs[i] = matrix_row
f_hmm_file.close()
seq_dict = get_fasta_dict(input_file)
emit_str = seq_dict.values()[0] # there's only 1
print "Done reading sequence of length ", len(emit_str)
# Run the forward algorithm
forward = run_forward(states, initial_probs, transitions, emitted_symbols, emit_probs, emit_str)
# Run the backward algorithm
backward = run_backward(states, initial_probs, transitions, emitted_symbols, emit_probs, emit_str)
# Calculate the posterior probabilities
# Initializing the posterior 2D matrices
posterior = [[float(0) for _ in range(K)] for _ in range(len(emit_str))]
for i in range(len(emit_str)):
# Did not normalize the probabilities (i.e., did not divide by P(X)),
# because we will only use these probabilities to compare
# posterior[i][0] versus posterior[i][1]
for k in range(K):
posterior[i][k] = forward[i][k] + backward[i][k]
# Print the decoded results
printout = []
for i in range(len(posterior)):
max_at_index = float('-inf')
max_k = float('-inf')
for j in range(len(posterior[i])):
if posterior[i][j] > max_at_index:
max_at_index = posterior[i][j]
max_k = j
printout.append(max_k)
# You could print it out, but it's very long:
# print "".join([str(x) for x in printout])
prev_state = -1
prev_state_index = - 1
print
print "start\tstop\tstate"
for i in range(len(printout)):
if i == 0:
prev_state = printout[i]
prev_state_index = 0
else:
if prev_state != printout[i]:
print str(prev_state_index+1) + "\t" + str(i) + "\t" + "state " + str(prev_state+1)
prev_state = printout[i]
prev_state_index = i
# print the last sequence here!
if prev_state_index < len(printout) - 1:
print str(prev_state_index+1) + "\t" + str(len(printout)) + "\t" + "state " + str(prev_state+1)
def posterior_decoding():
hmm_file = raw_input("Enter the name of the HMM file:").strip()
sys.stdout.flush()
input_file = raw_input("Enter the name of the input file:").strip()
f_in_file = open(input_file)
f_hmm_file = open(hmm_file)
if f_in_file is None:
sys.exit("Can't open HMM file: " + hmm_file)
if f_hmm_file is None:
sys.exit("Can't open file: " + input_file)
# read the state names and number
states = f_hmm_file.readline().split()
K = len(states)
#print("Our states are: " + str(states))
#print("The number K of states is " + str(K))
# read the initial probabilities
probs = f_hmm_file.readline().split()
initial_probs = [float(prob) for prob in probs]
#print("The initial probability of each of our states is " + str(initial_probs))
# read the transition matrix
transitions = [None for _ in range(K)]
for i in range(K):
matrix_row_arry = f_hmm_file.readline().split()
matrix_row = [float(trans_prob) for trans_prob in matrix_row_arry]
transitions[i] = matrix_row
#print("Our transition matrix between states is " + str(transitions))
# read the emission symbols
emission_symbols = f_hmm_file.readline().split()
#print("Our options for emitted symbols are " + str(emission_symbols))
# read the emission probability matrix
emit_probs = [None for _ in range(K)]
for i in range(K):
matrix_row_arry = f_hmm_file.readline().split()
matrix_row = [float(emit_prob) for emit_prob in matrix_row_arry]
emit_probs[i] = matrix_row
#print("Our emission probabilities are " + str(emit_probs))
f_hmm_file.close()
seq_dict = get_fasta_dict(input_file)
emit_str = seq_dict.values()[0] # there's only 1
print "Done reading sequence of length ", len(emit_str)
# Run the forward algorithm
forward = run_forward(states, initial_probs, transitions, emission_symbols,
emit_probs, emit_str)
# Run the backward algorithm
backward = run_backward(states, initial_probs, transitions,
emission_symbols, emit_probs, emit_str)
# Calculate the posterior probabilities
# Initializing the posterior 2D matrices
posterior = [[float(0) for _ in range(K)] for _ in range(len(emit_str))]
for i in range(len(emit_str)):
# Did not normalize the probabilities (i.e., did not divide by P(X)),
# because we will only use these probabilities to compare
# posterior[i][0] versus posterior[i][1]
for k in range(K):
posterior[i][k] = forward[i][k] + backward[i][k]
# Print out the decoded results
#print("Final posterior matrix is " + str(posterior))
#Initialize an output path
path = ""
#Iterate in reverse order through the characters of our string
for i in range(len(emit_str) - 1, -1, -1):
#Initialize a maximum probability and a state index
maxProb = float('-inf')
stateIndex = 0
#Iterate through the potential states for this character
for k in range(K):
#Find the maximum viterbi value, and based off of this value, get the corresponding state
#print("Checking row " + str(k))
if (posterior[i][k] > maxProb):
maxProb = posterior[i][k]
stateIndex = k
path = path + str(stateIndex + 1)
#print("Adding " + str(stateIndex+1))
#For the first value in our output path, save out an additional state correspodning the row of the viterbi value we chose
#print(path)
#print(emit_str)
#print(path[::-1])
#Return and print our output in terms of the format requested (see formatPath method)
output = formatPath(path[::-1], states)
print("Output is " + str(output))
return output
def posterior_decoding():
hmm_file = raw_input("Enter the name of the HMM file:").strip()
sys.stdout.flush()
input_file = raw_input("Enter the name of the input file:").strip()
f_in_file = open(input_file)
f_hmm_file = open(hmm_file)
if f_in_file is None:
sys.exit("Can't open HMM file: " + hmm_file)
if f_hmm_file is None:
sys.exit("Can't open file: " + input_file)
# read the state names and number
states = f_hmm_file.readline().split()
K = len(states)
# read the initial probabilities
probs = f_hmm_file.readline().split()
initial_probs = [float(prob) for prob in probs]
# read the transition matrix
transitions = [None for _ in range(K)]
for i in range(K):
matrix_row_arry = f_hmm_file.readline().split()
matrix_row = [float(trans_prob) for trans_prob in matrix_row_arry]
transitions[i] = matrix_row
# read the emitted symbols
emitted_symbols = f_hmm_file.readline().split()
# read the emission probability matrix
emit_probs = [None for _ in range(K)]
for i in range(K):
matrix_row_arry = f_hmm_file.readline().split()
matrix_row = [float(emit_prob) for emit_prob in matrix_row_arry]
emit_probs[i] = matrix_row
f_hmm_file.close()
seq_dict = get_fasta_dict(input_file)
emit_str = seq_dict.values()[0] # there's only 1
print "Done reading sequence of length ", len(emit_str)
# Run the forward algorithm
forward = run_forward(states, initial_probs, transitions, emitted_symbols, emit_probs, emit_str)
# Run the backward algorithm
backward = run_backward(states, initial_probs, transitions, emitted_symbols, emit_probs, emit_str)
# Calculate the posterior probabilities
# Initializing the posterior 2D matrices
posterior = [[float(0) for _ in range(K)] for _ in range(len(emit_str))]
for i in range(len(emit_str)):
# Did not normalize the probabilities (i.e., did not divide by P(X)),
# because we will only use these probabilities to compare
# posterior[i][0] versus posterior[i][1]
for k in range(K):
posterior[i][k] = forward[i][k] + backward[i][k]
# Print out the decoded results
path = []
for i in range(len(posterior)):
path.append(posterior[i].index(max(posterior[i])))
states_array = []
state_start_index = 0
previous_state = path[0]
for i, state in enumerate(path):
if(state!=previous_state):
states_array.append((state_start_index + 1, i, previous_state))
state_start_index = i
previous_state = state
states_array.append((state_start_index + 1, i + 1, previous_state))
print "Start Stop State"
state1_count = 0
state2_count = 0
for trip in states_array:
if(trip[2]==1):
state2_count = state2_count + 1
if(trip[2]==0):
state1_count = state1_count + 1
print trip[0], trip[1], states[trip[2]]
print "total distinct state1 regions: " + str(state1_count)
print "total distinct state2 regions: " + str(state2_count)
