def testRoundtripWithBeadCalibration(self):
self.cwd = os.path.dirname(os.path.abspath(__file__))
self.ex = flow.ImportOp(conditions={
'Dox': 'float'
},
tubes=[
flow.Tube(file=self.cwd +
'/data/tasbe/rby.fcs',
conditions={'Dox': 1.0})
]).apply()
op = flow.BeadCalibrationOp(
units={"PE-Tx-Red-YG-A": "MEPTR"},
beads_file=self.cwd + '/data/tasbe/beads.fcs',
beads=flow.BeadCalibrationOp.BEADS[
"Spherotech RCP-30-5A Lot AA01-AA04, AB01, AB02, AC01, GAA01-R"]
)
op.estimate(self.ex)
ex2 = op.apply(self.ex)
flow.ExportFCS(path=self.directory, by=['Dox']).export(ex2)
tube1 = flow.Tube(file=self.directory / 'Dox_1.0.fcs',
conditions={"Dox": 1.0})
ex_rt = flow.ImportOp(conditions={
'Dox': 'float'
}, tubes=[tube1]).apply()
self.assertNotIn('voltage', ex_rt.metadata['PE-Tx-Red-YG-A'])
def setUp(self):
import os
self.cwd = os.path.dirname(os.path.abspath(__file__))
self.ex = flow.ImportOp(conditions = {},
tubes = [flow.Tube(file = self.cwd + '/data/tasbe/rby.fcs',
conditions = {})]).apply()
self.op = flow.BeadCalibrationOp(
units = {"PE-Tx-Red-YG-A" : "MEPTR"},
beads_file = self.cwd + '/data/tasbe/beads.fcs',
beads = flow.BeadCalibrationOp.BEADS["Spherotech RCP-30-5A Lot AA01-AA04, AB01, AB02, AC01, GAA01-R"])
self.op.estimate(self.ex)