def load(virus):
if virus == 'h1':
from escape import load_doud2018
seq, seqs_escape = load_doud2018()
train_fname = 'target/flu/clusters/all_h1.fasta'
mut_fname = 'target/flu/mutation/mutations_h1.fa'
anchor_id = ('gb:LC333185|ncbiId:BBB04702.1|UniProtKB:-N/A-|'
'Organism:Influenza')
elif virus == 'h3':
from escape import load_lee2019
seq, seqs_escape = load_lee2019()
train_fname = 'target/flu/clusters/all_h3.fasta'
mut_fname = 'target/flu/mutation/mutations_h3.fa'
anchor_id = 'Reference_Perth2009_HA_coding_sequence'
elif virus == 'bg505':
from escape import load_dingens2019
seq, seqs_escape = load_dingens2019()
train_fname = 'target/hiv/clusters/all_BG505.fasta'
mut_fname = 'target/hiv/mutation/mutations_hiv.fa'
anchor_id = 'A1.KE.-.BG505_W6M_ENV_C2.DQ208458'
elif virus == 'sarscov2':
from escape import load_baum2020
seq, seqs_escape = load_baum2020()
train_fname = 'target/cov/clusters/all_sarscov2.fasta'
mut_fname = 'target/cov/mutation/mutations_sarscov2.fa'
anchor_id = 'YP_009724390.1'
elif virus == 'cov2rbd':
from escape import load_greaney2020
seq, seqs_escape = load_greaney2020()
train_fname = 'target/cov/clusters/all_sarscov2.fasta'
mut_fname = 'target/cov/mutation/mutations_sarscov2.fa'
anchor_id = 'YP_009724390.1'
else:
raise ValueError('invalid option {}'.format(virus))
return seq, seqs_escape, train_fname, mut_fname, anchor_id
def cached_escape(cache_fname,
beta,
cutoff=None,
expr_cutoff=None,
bind_cutoff=None,
plot=True,
namespace='semantics'):
if 'flu_h1' in cache_fname:
from escape import load_doud2018
if cutoff is None:
wt_seq, seqs_escape = load_doud2018()
else:
wt_seq, seqs_escape = load_doud2018(survival_cutoff=cutoff)
elif 'flu_h3' in cache_fname:
from escape import load_lee2019
if cutoff is None:
wt_seq, seqs_escape = load_lee2019()
else:
wt_seq, seqs_escape = load_lee2019(survival_cutoff=cutoff)
elif 'hiv' in cache_fname:
from escape import load_dingens2019
if cutoff is None:
wt_seq, seqs_escape = load_dingens2019()
else:
wt_seq, seqs_escape = load_dingens2019(survival_cutoff=cutoff)
elif '_cov_' in cache_fname:
from escape import load_baum2020
wt_seq, seqs_escape = load_baum2020()
elif 'cov2rbd' in cache_fname:
from escape import load_greaney2020
if cutoff is None:
wt_seq, seqs_escape = load_greaney2020()
elif expr_cutoff is not None:
wt_seq, seqs_escape = load_greaney2020(expr_cutoff=expr_cutoff)
else:
wt_seq, seqs_escape = load_greaney2020(survival_cutoff=cutoff)
else:
raise ValueError('invalid option {}'.format(cache_fname))
prob, change, escape_idx, viable_idx = [], [], [], []
with open(cache_fname) as f:
f.readline()
for line in f:
fields = line.rstrip().split('\t')
pos = int(fields[0])
if 'rbd' in cache_fname:
if pos < 330 or pos > 530:
continue
if fields[2] in {'U', 'B', 'J', 'X', 'Z'}:
continue
aa_wt = fields[1]
aa_mut = fields[2]
assert (wt_seq[pos] == aa_wt)
mut_seq = wt_seq[:pos] + aa_mut + wt_seq[pos + 1:]
if mut_seq not in seqs_escape:
continue
prob.append(float(fields[3]))
change.append(float(fields[4]))
viable_idx.append(fields[5] == 'True')
escape_idx.append(
(mut_seq in seqs_escape)
and (sum([m['significant']
for m in seqs_escape[mut_seq]]) > 0))
prob, orig_prob = np.array(prob), np.array(prob)
change, orig_change = np.array(change), np.array(change)
escape_idx = np.array(escape_idx)
viable_idx = np.array(viable_idx)
acquisition = ss.rankdata(change) + (beta * ss.rankdata(prob))
pos_change_idx = change > 0
pos_change_escape_idx = np.logical_and(pos_change_idx, escape_idx)
escape_prob = prob[pos_change_escape_idx]
escape_change = change[pos_change_escape_idx]
prob = prob[pos_change_idx]
change = change[pos_change_idx]
log_prob, log_change = np.log10(prob), np.log10(change)
log_escape_prob, log_escape_change = (np.log10(escape_prob),
np.log10(escape_change))
if plot:
mkdir_p('figures')
plt.figure()
plt.scatter(log_prob,
log_change,
c=acquisition[pos_change_idx],
cmap='viridis',
alpha=0.3)
plt.scatter(log_escape_prob,
log_escape_change,
c='red',
alpha=0.5,
marker='x')
plt.xlabel(r'$ \log_{10}(\hat{p}(x_i | \mathbf{x}_{[N] ∖ \{i\} })) $')
plt.ylabel(r'$ \log_{10}(\Delta \mathbf{\hat{z}}) $')
plt.savefig('figures/{}_acquisition.png'.format(namespace), dpi=300)
plt.close()
rand_idx = np.random.choice(len(prob), len(escape_prob))
plt.figure()
plt.scatter(log_prob,
log_change,
c=acquisition[pos_change_idx],
cmap='viridis',
alpha=0.3)
plt.scatter(log_prob[rand_idx],
log_change[rand_idx],
c='red',
alpha=0.5,
marker='x')
plt.xlabel(r'$ \log_{10}(\hat{p}(x_i | \mathbf{x}_{[N] ∖ \{i\} })) $')
plt.ylabel(r'$ \log_{10}(\Delta \mathbf{\hat{z}}) $')
plt.savefig('figures/{}_acquisition_rand.png'.format(namespace),
dpi=300)
plt.close()
if len(escape_prob) == 0:
print('No escape mutations found.')
return
acq_argsort = ss.rankdata(-acquisition)
escape_rank_dist = acq_argsort[escape_idx]
size = len(prob)
print('Number of escape seqs: {} / {}'.format(len(escape_rank_dist),
sum(escape_idx)))
print('Mean rank: {} / {}'.format(np.mean(escape_rank_dist), size))
print('Median rank: {} / {}'.format(np.median(escape_rank_dist), size))
print('Min rank: {} / {}'.format(np.min(escape_rank_dist), size))
print('Max rank: {} / {}'.format(np.max(escape_rank_dist), size))
print('Rank stdev: {} / {}'.format(np.std(escape_rank_dist), size))
max_consider = len(prob)
n_consider = np.array([i + 1 for i in range(max_consider)])
n_escape = np.array(
[sum(escape_rank_dist <= i + 1) for i in range(max_consider)])
norm = max(n_consider) * max(n_escape)
norm_auc = auc(n_consider, n_escape) / norm
escape_rank_prob = ss.rankdata(-orig_prob)[escape_idx]
n_escape_prob = np.array(
[sum(escape_rank_prob <= i + 1) for i in range(max_consider)])
norm_auc_prob = auc(n_consider, n_escape_prob) / norm
escape_rank_change = ss.rankdata(-orig_change)[escape_idx]
n_escape_change = np.array(
[sum(escape_rank_change <= i + 1) for i in range(max_consider)])
norm_auc_change = auc(n_consider, n_escape_change) / norm
if plot:
plt.figure()
plt.plot(n_consider, n_escape)
plt.plot(n_consider, n_escape_change, c='C0', linestyle='-.')
plt.plot(n_consider, n_escape_prob, c='C0', linestyle=':')
plt.plot(n_consider,
n_consider * (len(escape_prob) / len(prob)),
c='gray',
linestyle='--')
plt.xlabel(r'$ \log_{10}() $')
plt.ylabel(r'$ \log_{10}(\Delta \mathbf{\hat{z}}) $')
plt.legend([
r'$ \Delta \mathbf{\hat{z}} + ' +
r'\beta \hat{p}(x_i | \mathbf{x}_{[N] ∖ \{i\} }) $,' +
(' AUC = {:.3f}'.format(norm_auc)),
r'$ \Delta \mathbf{\hat{z}} $ only,' +
(' AUC = {:.3f}'.format(norm_auc_change)),
r'$ \hat{p}(x_i | \mathbf{x}_{[N] ∖ \{i\} }) $ only,' +
(' AUC = {:.3f}'.format(norm_auc_prob)),
'Random guessing, AUC = 0.500'
])
plt.xlabel('Top N')
plt.ylabel('Number of escape mutations in top N')
plt.savefig('figures/{}_consider_escape.png'.format(namespace),
dpi=300)
plt.close()
print('Escape semantics, beta = {} [{}]'.format(beta, namespace))
norm_auc_p = compute_p(norm_auc, sum(escape_idx), len(escape_idx))
print('AUC (CSCS): {}, P = {}'.format(norm_auc, norm_auc_p))
print('AUC (semantic change only): {}'.format(norm_auc_change))
print('AUC (grammaticality only): {}'.format(norm_auc_prob))
print('{:.4g} (mean log prob), {:.4g} (mean log prob escape), '
'{:.4g} (p-value)'.format(
log_prob.mean(), log_escape_prob.mean(),
ss.mannwhitneyu(log_prob,
log_escape_prob,
alternative='two-sided')[1]))
print('{:.4g} (mean log change), {:.4g} (mean log change escape), '
'{:.4g} (p-value)'.format(
change.mean(), escape_change.mean(),
ss.mannwhitneyu(change, escape_change,
alternative='two-sided')[1]))
from escape import load_baum2020, load_greaney2020
tprint('Baum et al. 2020...')
seq_to_mutate, seqs_escape = load_baum2020()
analyze_semantics(
args,
model,
vocabulary,
seq_to_mutate,
seqs_escape,
comb_batch=5000,
prob_cutoff=0,
beta=1.,
plot_acquisition=True,
)
tprint('Greaney et al. 2020...')
seq_to_mutate, seqs_escape = load_greaney2020()
analyze_semantics(
args,
model,
vocabulary,
seq_to_mutate,
seqs_escape,
comb_batch=5000,
min_pos=318,
max_pos=540, # Restrict to RBD.
prob_cutoff=0,
beta=1.,
plot_acquisition=True,
plot_namespace='cov2rbd')
if args.combfit: