예제 #1
0
	def test_03(self):
		"""
		Check the duplication removal - simple test; 2 identical fusions
		"""
		
		args = CLI(['-m','subset','--no-strand-specific-matching','-s',''])
		
		fusion_1 = Fusion("chr1","chr2",15000,20000,"+","+","Experiment","",True)
		fusion_2 = Fusion("chr1","chr2",15000,20000,"+","+","Experiment","",True)
		
		experiment = FusionDetectionExperiment("Experiment_1")
		
		experiment.add_fusion(fusion_1)
		experiment.add_fusion(fusion_2)
		
		self.assertEqual(len(experiment), 2)
		
		genes = ParseBED("tests/data/test_FusionDetectionExperiment.TestFusionDetectionExperiment.test_03.bed","hg18", 200000)
		experiment.annotate_genes(genes)
		
		experiment.remove_duplicates(args)
		
		self.assertEqual(len(experiment), 1)
		
		for fusion in experiment:
			self.assertEqual(len(fusion.annotated_genes_left), 1)
			self.assertEqual(len(fusion.annotated_genes_right), 1)
예제 #2
0
	def test_08(self):
		"""
		Check the duplication removal - 2 fusions where one is missing
		annotations -> one should be lost because it isn't gene
		spanning
		"""
		args = CLI(['-m','subset','--no-strand-specific-matching','-s',''])
		
		fusion_1 = Fusion("chr1","chr2",15000,20000,"+","+","Experiment","",True)
		fusion_2 = Fusion("chr1","chr2",15000,30000,"+","+","Experiment","",True)
		
		experiment = FusionDetectionExperiment("Experiment_1")
		experiment.add_fusion(fusion_1)
		experiment.add_fusion(fusion_2)
		
		self.assertEqual(len(experiment), 2)
		
		genes = ParseBED("tests/data/test_FusionDetectionExperiment.TestFusionDetectionExperiment.test_08.bed","hg18", 200000)
		experiment.annotate_genes(genes)
		experiment.remove_duplicates(args)
		
		self.assertEqual(len(experiment), 1)
		
		for fusion in experiment:
			self.assertEqual(len(fusion.annotated_genes_left), 1)
			self.assertEqual(len(fusion.annotated_genes_right), 1)
	def test_04(self):
		"""
#1:
        -->                     <--
        break1                    break2
        |                         |
[ --- Gene A --- ]        [ --- Gene B --- ]

#2:
      <--                         -->
        break1                    break2
        |                         |
[ --- Gene A --- ]        [ --- Gene B --- ]

Ensure the strand of the merged fusion is not set!
"""
		gene_A = Gene("A", False)
		gene_B = Gene("B", False)
		
		genes = GeneAnnotation("hg19")
		genes.add_annotation(gene_A,"1",10000,20000)
		genes.add_annotation(gene_B,"1",80000,90000)
		
		fusion_1 = Fusion("chr1","chr1",15000,85000,"+","-","Experiment_1",3,True)
		fusion_2 = Fusion("chr1","chr1",15000,85000,"-","+","Experiment_2",4,True)
		
		experiment_1 = FusionDetectionExperiment("Experiment_1")
		experiment_1.add_fusion(fusion_1)
		experiment_1.annotate_genes(genes)
		
		experiment_2 = FusionDetectionExperiment("Experiment_2")
		experiment_2.add_fusion(fusion_2)
		experiment_2.annotate_genes(genes)
		
		args = CLI(['-f','summary','--no-strand-specific-matching','-s',''])
		overlapping_complex = OverlapComplex()
		overlapping_complex.add_experiment(experiment_1)
		overlapping_complex.add_experiment(experiment_2)
		overlap = overlapping_complex.overlay_fusions(True,False,args)
		
		self.assertEqual(overlap[0][0].left_strand, None)
		self.assertEqual(overlap[0][0].right_strand, None)
		
		args = CLI(['-f','summary','--no-strand-specific-matching','-s',''])
		overlapping_complex = OverlapComplex()
		overlapping_complex.add_experiment(experiment_1)
		overlapping_complex.add_experiment(experiment_1)
		overlap = overlapping_complex.overlay_fusions(True,False,args)
		
		self.assertNotEqual(overlap[0][0].left_strand, None)
		self.assertNotEqual(overlap[0][0].right_strand, None)
		
		args = CLI(['-f','summary','--no-strand-specific-matching','-s',''])
		overlapping_complex = OverlapComplex()
		overlapping_complex.add_experiment(experiment_2)
		overlapping_complex.add_experiment(experiment_2)
		overlap = overlapping_complex.overlay_fusions(True,False,args)
		
		self.assertNotEqual(overlap[0][0].left_strand, None)
		self.assertNotEqual(overlap[0][0].right_strand, None)
	def test_08(self):
		"""
		Check the duplication removal - 2 fusions where one is missing
		annotations -> one should be lost because it isn't gene
		spanning
		"""
		args = CLI(['-m','subset','--no-strand-specific-matching','-s',''])
		
		fusion_1 = Fusion("chr1","chr2",15000,20000,"+","+","Experiment","",True)
		fusion_2 = Fusion("chr1","chr2",15000,30000,"+","+","Experiment","",True)
		
		experiment = FusionDetectionExperiment("Experiment_1")
		experiment.add_fusion(fusion_1)
		experiment.add_fusion(fusion_2)
		
		self.assertEqual(len(experiment), 2)
		
		genes = ParseBED("tests/data/test_FusionDetectionExperiment.TestFusionDetectionExperiment.test_08.bed","hg18", 200000)
		experiment.annotate_genes(genes)
		experiment.remove_duplicates(args)
		
		self.assertEqual(len(experiment), 1)
		
		for fusion in experiment:
			self.assertEqual(len(fusion.annotated_genes_left), 1)
			self.assertEqual(len(fusion.annotated_genes_right), 1)
	def test_05(self):
		"""
		Check the duplication removal - 2 fusions; one has two
		overlapping left genes, the other only one, and for right is
		vice versa. The 1 gene is always a subset of the others 2 genes
		and must therefore be treated as identical annotations
		"""
		
		args = CLI(['-m','subset','--no-strand-specific-matching','-s',''])
		
		fusion_1 = Fusion("chr1","chr2",15000,20050,"+","+","Experiment","",True)#(1A):(2A,2B)
		fusion_2 = Fusion("chr1","chr2",15050,20000,"+","+","Experiment","",True)#(1A,1B):(2A)
		
		experiment = FusionDetectionExperiment("Experiment_1")
		experiment.add_fusion(fusion_1)
		experiment.add_fusion(fusion_2)
		
		self.assertEqual(len(experiment), 2)
		
		genes = ParseBED("tests/data/test_FusionDetectionExperiment.TestFusionDetectionExperiment.test_05.bed","hg18", 200000)
		experiment.annotate_genes(genes)
		experiment.remove_duplicates(args)
		
		self.assertEqual(len(experiment), 1)
		
		for fusion in experiment:
			self.assertEqual(len(fusion.annotated_genes_left), 1)		# subset = (1A)
			self.assertEqual(len(fusion.annotated_genes_right), 1)		# subset = (2A)
	def test_03(self):
		"""
		Check the duplication removal - simple test; 2 identical fusions
		"""
		
		args = CLI(['-m','subset','--no-strand-specific-matching','-s',''])
		
		fusion_1 = Fusion("chr1","chr2",15000,20000,"+","+","Experiment","",True)
		fusion_2 = Fusion("chr1","chr2",15000,20000,"+","+","Experiment","",True)
		
		experiment = FusionDetectionExperiment("Experiment_1")
		
		experiment.add_fusion(fusion_1)
		experiment.add_fusion(fusion_2)
		
		self.assertEqual(len(experiment), 2)
		
		genes = ParseBED("tests/data/test_FusionDetectionExperiment.TestFusionDetectionExperiment.test_03.bed","hg18", 200000)
		experiment.annotate_genes(genes)
		
		experiment.remove_duplicates(args)
		
		self.assertEqual(len(experiment), 1)
		
		for fusion in experiment:
			self.assertEqual(len(fusion.annotated_genes_left), 1)
			self.assertEqual(len(fusion.annotated_genes_right), 1)
예제 #7
0
	def test_05(self):
		"""
		Check the duplication removal - 2 fusions; one has two
		overlapping left genes, the other only one, and for right is
		vice versa. The 1 gene is always a subset of the others 2 genes
		and must therefore be treated as identical annotations
		"""
		
		args = CLI(['-m','subset','--no-strand-specific-matching','-s',''])
		
		fusion_1 = Fusion("chr1","chr2",15000,20050,"+","+","Experiment","",True)#(1A):(2A,2B)
		fusion_2 = Fusion("chr1","chr2",15050,20000,"+","+","Experiment","",True)#(1A,1B):(2A)
		
		experiment = FusionDetectionExperiment("Experiment_1")
		experiment.add_fusion(fusion_1)
		experiment.add_fusion(fusion_2)
		
		self.assertEqual(len(experiment), 2)
		
		genes = ParseBED("tests/data/test_FusionDetectionExperiment.TestFusionDetectionExperiment.test_05.bed","hg18", 200000)
		experiment.annotate_genes(genes)
		experiment.remove_duplicates(args)
		
		self.assertEqual(len(experiment), 1)
		
		for fusion in experiment:
			self.assertEqual(len(fusion.annotated_genes_left), 1)		# subset = (1A)
			self.assertEqual(len(fusion.annotated_genes_right), 1)		# subset = (2A)
예제 #8
0
    def test_01(self):
        """
#1:
        break1                    break2
        |                         |
[ --- Gene A --- ]        [ --- Gene B --- ]

#2:
        break1                    break2
        |                         |
[ --- Gene B --- ]        [ --- Gene A --- ]
		"""

        args_on = CLI([
            '--acceptor-donor-order-specific-matching', '-f', 'summary', '-s',
            ''
        ])
        args_off = CLI(['-f', 'summary', '-s', ''])

        gene_A = Gene("A", False)
        gene_B = Gene("B", False)

        genes = GeneAnnotation("hg19")
        genes.add_annotation(gene_A, "1", 10000, 20000)
        genes.add_annotation(gene_B, "1", 80000, 90000)

        fusion_1 = Fusion("chr1", "chr1", 15000, 85000, "+", "+",
                          "Experiment_1", "1", True)
        experiment_1 = FusionDetectionExperiment("Experiment_1")
        experiment_1.add_fusion(fusion_1)
        experiment_1.annotate_genes(genes)

        fusion_2 = Fusion("chr1", "chr1", 85000, 15000, "+", "+",
                          "Experiment_2", "2", True)
        experiment_2 = FusionDetectionExperiment("Experiment_2")
        experiment_2.add_fusion(fusion_2)
        experiment_2.annotate_genes(genes)

        self.assertEqual(len(fusion_1.annotated_genes_left), 1)
        self.assertEqual(len(fusion_2.annotated_genes_left), 1)

        self.assertEqual(fusion_1.acceptor_donor_direction,
                         AD_DIRECTION_FORWARD)
        self.assertEqual(fusion_2.acceptor_donor_direction,
                         AD_DIRECTION_REVERSE)

        overlapping_complex = OverlapComplex()
        overlapping_complex.add_experiment(experiment_1)
        overlapping_complex.add_experiment(experiment_2)
        overlap = overlapping_complex.overlay_fusions(True, False, args_on)

        self.assertEqual(len(overlap[0]), 0)

        overlapping_complex = OverlapComplex()
        overlapping_complex.add_experiment(experiment_1)
        overlapping_complex.add_experiment(experiment_2)
        overlap = overlapping_complex.overlay_fusions(True, False, args_off)

        self.assertEqual(len(overlap[0]), 1)
	def test_01(self):
		"""
#1:
        break1                    break2
        |                         |
[ --- Gene A --- ]        [ --- Gene B --- ]

#2:
        break1                    break2
        |                         |
[ --- Gene B --- ]        [ --- Gene A --- ]
		"""
		
		args_on = CLI(['--acceptor-donor-order-specific-matching','-f','summary','-s',''])
		args_off = CLI(['-f','summary','-s',''])
		
		gene_A = Gene("A", False)
		gene_B = Gene("B", False)
		
		genes = GeneAnnotation("hg19")
		genes.add_annotation(gene_A,"1",10000,20000)
		genes.add_annotation(gene_B,"1",80000,90000)
		
		fusion_1 = Fusion("chr1","chr1",15000,85000,None,None,"+","+","Experiment_1")
		fusion_1.add_location({'left':[fusion_1.get_left_chromosome(), fusion_1.get_left_break_position()], 'right':[fusion_1.get_right_chromosome(), fusion_1.get_right_break_position()], 'id':1, 'dataset':fusion_1.dataset_name })
		experiment_1 = FusionDetectionExperiment("Experiment_1")
		experiment_1.add_fusion(fusion_1)
		experiment_1.annotate_genes(genes)
		
		fusion_2 = Fusion("chr1","chr1",85000,15000,None,None,"+","+","Experiment_2")
		fusion_2.add_location({ 'left':[fusion_2.get_left_chromosome(), fusion_2.get_left_break_position()], 'right':[fusion_2.get_right_chromosome(), fusion_2.get_right_break_position()], 'id':2, 'dataset':fusion_2.dataset_name })
		experiment_2 = FusionDetectionExperiment("Experiment_2")
		experiment_2.add_fusion(fusion_2)
		experiment_2.annotate_genes(genes)
		
		self.assertEqual(len(fusion_1.annotated_genes_left) , 1)
		self.assertEqual(len(fusion_2.annotated_genes_left) , 1)
		
		self.assertEqual( fusion_1.acceptor_donor_direction , AD_DIRECTION_FORWARD )
		self.assertEqual( fusion_2.acceptor_donor_direction , AD_DIRECTION_REVERSE )
		
		
		overlapping_complex = OverlapComplex()
		overlapping_complex.add_experiment(experiment_1)
		overlapping_complex.add_experiment(experiment_2)
		overlap = overlapping_complex.overlay_fusions(True,False,args_on)
		
		self.assertEqual(len(overlap[0]), 0)
		
		
		overlapping_complex = OverlapComplex()
		overlapping_complex.add_experiment(experiment_1)
		overlapping_complex.add_experiment(experiment_2)
		overlap = overlapping_complex.overlay_fusions(True,False,args_off)
		
		self.assertEqual(len(overlap[0]), 1)
	def test_10(self):
		"""
		May not have overlap:
		{1} = a,b,c
		{2} = a,d
		
		Break {1}:
		                    |
		Break {2}:          :
		       |            :
		a: [   :            :        ]
		b:     :  [         :        ]
		c:     :     [      :        ]
		d: [   : ]          :
		"""
		args = CLI(['-m','subset','--no-strand-specific-matching','-s',''])
		
		fusion_1 = Fusion("chr1","chr2",15000,30000,"+","+","Experiment","",True)
		fusion_2 = Fusion("chr1","chr2",15000,25000,"+","+","Experiment","",True)
		
		experiment = FusionDetectionExperiment("Experiment_1")
		
		experiment.add_fusion(fusion_1)
		experiment.add_fusion(fusion_2)
		
		self.assertEqual(len(experiment), 2)
		self.assertEqual(str(experiment), "---------------------\nShowing experiment: Experiment_1\n\nFusion '' (from dataset 'Experiment'): chr1:15000(+)->chr2:30000(+)\nFusion '' (from dataset 'Experiment'): chr1:15000(+)->chr2:25000(+)\n---------------------\n")
		
		
		genes = ParseBED("tests/data/test_FusionDetectionExperiment.TestFusionDetectionExperiment.test_10.bed","hg18", 200000)
		
		experiment.annotate_genes(genes)
		
		for fusion in experiment:
			if(fusion.get_right_break_position() == 30000):
				self.assertEqual(len(fusion.annotated_genes_right), 3)
				self.assertTrue("NM_00002A" in [str(gene_name) for gene_name in fusion.annotated_genes_right])
				self.assertTrue("NM_00002B" in [str(gene_name) for gene_name in fusion.annotated_genes_right])
				self.assertTrue("NM_00002C" in [str(gene_name) for gene_name in fusion.annotated_genes_right])
			if(fusion.get_right_break_position() == 25000):
				self.assertEqual(len(fusion.annotated_genes_right), 2)
				self.assertTrue("NM_00002A" in [str(gene_name) for gene_name in fusion.annotated_genes_right])
				self.assertTrue("NM_00002D" in [str(gene_name) for gene_name in fusion.annotated_genes_right])
		
		experiment.remove_duplicates(args)
		
		self.assertEqual(len(experiment), 2)
	def test_12(self):
		"""
		{a} = 1 , 4 , 5
		{b} = 2 , 6
		{c} = 7 , 8
		{d} = 3
		
		   | f1 | f2 | f3 | f4 | f5 | f6 | f7 | f8 |
		---+----+----+----+----+----+----+----+----+
		f1 | *  |    |    | a  | a  |    |    |    |
		---+----+----+----+----+----+----+----+----+
		f2 |    | *  |    |    |    | b  |    |    |
		---+----+----+----+----+----+----+----+----+
		f3 |    |    | d  |    |    |    |    |    |
		---+----+----+----+----+----+----+----+----+
		f4 | a  |    |    | *  | a  |    |    |    |
		---+----+----+----+----+----+----+----+----+
		f5 | a  |    |    | a  | *  |    |    |    |
		---+----+----+----+----+----+----+----+----+
		f6 |    | b  |    |    |    | *  |    |    |
		---+----+----+----+----+----+----+----+----+
		f7 |    |    |    |    |    |    | *  | d  |
		---+----+----+----+----+----+----+----+----+
		f8 |    |    |    |    |    |    | c  | *  |
		---+----+----+----+----+----+----+----+----+
		"""
		args = CLI(['-m','subset','--no-strand-specific-matching','-s',''])
		
		fusion_1 = Fusion("chr1","chr1",15010,80040,"+","+","Experiment","",True)
		fusion_2 = Fusion("chr2","chr2",15030,80030,"+","+","Experiment","",True)
		fusion_3 = Fusion("chr4","chr4",15050,80070,"+","+","Experiment","",True)
		fusion_4 = Fusion("chr1","chr1",15060,80010,"+","+","Experiment","",True)
		fusion_5 = Fusion("chr1","chr1",15020,80050,"+","+","Experiment","",True)
		fusion_6 = Fusion("chr2","chr2",15080,80080,"+","+","Experiment","",True)
		fusion_7 = Fusion("chr3","chr3",15040,80020,"+","+","Experiment","",True)
		fusion_8 = Fusion("chr3","chr3",15070,80060,"+","+","Experiment","",True)
		
		experiment = FusionDetectionExperiment("Experiment_1")
		
		experiment.add_fusion(fusion_2)
		experiment.add_fusion(fusion_8)
		experiment.add_fusion(fusion_6)
		experiment.add_fusion(fusion_4)
		experiment.add_fusion(fusion_5)
		experiment.add_fusion(fusion_3)
		experiment.add_fusion(fusion_1)
		experiment.add_fusion(fusion_7)
		
		self.assertEqual(len(experiment), 8)
		
		genes = ParseBED("tests/data/test_FusionDetectionExperiment.TestFusionDetectionExperiment.test_12.bed","hg18", 200000)
		
		experiment.annotate_genes(genes)
		
		experiment.remove_duplicates(args)
		
		self.assertEqual(len(experiment), 4)
예제 #12
0
	def test_12(self):
		"""
		{a} = 1 , 4 , 5
		{b} = 2 , 6
		{c} = 7 , 8
		{d} = 3
		
		   | f1 | f2 | f3 | f4 | f5 | f6 | f7 | f8 |
		---+----+----+----+----+----+----+----+----+
		f1 | *  |    |    | a  | a  |    |    |    |
		---+----+----+----+----+----+----+----+----+
		f2 |    | *  |    |    |    | b  |    |    |
		---+----+----+----+----+----+----+----+----+
		f3 |    |    | d  |    |    |    |    |    |
		---+----+----+----+----+----+----+----+----+
		f4 | a  |    |    | *  | a  |    |    |    |
		---+----+----+----+----+----+----+----+----+
		f5 | a  |    |    | a  | *  |    |    |    |
		---+----+----+----+----+----+----+----+----+
		f6 |    | b  |    |    |    | *  |    |    |
		---+----+----+----+----+----+----+----+----+
		f7 |    |    |    |    |    |    | *  | d  |
		---+----+----+----+----+----+----+----+----+
		f8 |    |    |    |    |    |    | c  | *  |
		---+----+----+----+----+----+----+----+----+
		"""
		args = CLI(['-m','subset','--no-strand-specific-matching','-s',''])
		
		fusion_1 = Fusion("chr1","chr1",15010,80040,"+","+","Experiment","",True)
		fusion_2 = Fusion("chr2","chr2",15030,80030,"+","+","Experiment","",True)
		fusion_3 = Fusion("chr4","chr4",15050,80070,"+","+","Experiment","",True)
		fusion_4 = Fusion("chr1","chr1",15060,80010,"+","+","Experiment","",True)
		fusion_5 = Fusion("chr1","chr1",15020,80050,"+","+","Experiment","",True)
		fusion_6 = Fusion("chr2","chr2",15080,80080,"+","+","Experiment","",True)
		fusion_7 = Fusion("chr3","chr3",15040,80020,"+","+","Experiment","",True)
		fusion_8 = Fusion("chr3","chr3",15070,80060,"+","+","Experiment","",True)
		
		experiment = FusionDetectionExperiment("Experiment_1")
		
		experiment.add_fusion(fusion_2)
		experiment.add_fusion(fusion_8)
		experiment.add_fusion(fusion_6)
		experiment.add_fusion(fusion_4)
		experiment.add_fusion(fusion_5)
		experiment.add_fusion(fusion_3)
		experiment.add_fusion(fusion_1)
		experiment.add_fusion(fusion_7)
		
		self.assertEqual(len(experiment), 8)
		
		genes = ParseBED("tests/data/test_FusionDetectionExperiment.TestFusionDetectionExperiment.test_12.bed","hg18", 200000)
		
		experiment.annotate_genes(genes)
		
		experiment.remove_duplicates(args)
		
		self.assertEqual(len(experiment), 4)
예제 #13
0
	def test_11(self):
		"""
		May not have overlap:
		{1} = a,b,c
		{2} = a,b,d
		
		Break {1}:
		                    |
		Break {2}:          :
		       |            :
		a: [   :            :        ]
		b:   [ |            :  ]
		c:     :     [      :        ]
		d: [   : ]     
		"""
		args = CLI(['-m','subset','--no-strand-specific-matching','-s',''])
		
		fusion_1 = Fusion("chr1","chr2",15000,27500,"+","+","Experiment","",True)
		fusion_2 = Fusion("chr1","chr2",15000,25500,"+","+","Experiment","",True)
		
		experiment = FusionDetectionExperiment("Experiment_1")
		
		experiment.add_fusion(fusion_1)
		experiment.add_fusion(fusion_2)
		
		self.assertEqual(len(experiment), 2)
		
		genes = ParseBED("tests/data/test_FusionDetectionExperiment.TestFusionDetectionExperiment.test_11.bed","hg18", 200000)
		
		experiment.annotate_genes(genes)
		
		for fusion in experiment:
			if(fusion.get_right_break_position() == 30000):
				self.assertEqual(len(fusion.annotated_genes_right), 3)
				self.assertTrue("NM_00002A" in [str(gene_name) for gene_name in fusion.annotated_genes_right])
				self.assertTrue("NM_00002B" in [str(gene_name) for gene_name in fusion.annotated_genes_right])
				self.assertTrue("NM_00002C" in [str(gene_name) for gene_name in fusion.annotated_genes_right])
			if(fusion.get_right_break_position() == 25000):
				self.assertEqual(len(fusion.annotated_genes_right), 3)
				self.assertTrue("NM_00002A" in [str(gene_name) for gene_name in fusion.annotated_genes_right])
				self.assertTrue("NM_00002B" in [str(gene_name) for gene_name in fusion.annotated_genes_right])
				self.assertTrue("NM_00002D" in [str(gene_name) for gene_name in fusion.annotated_genes_right])
		
		experiment.remove_duplicates(args)
		
		self.assertEqual(len(experiment), 2)
예제 #14
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	def test_11(self):
		"""
		May not have overlap:
		{1} = a,b,c
		{2} = a,b,d
		
		Break {1}:
		                    |
		Break {2}:          :
		       |            :
		a: [   :            :        ]
		b:   [ |            :  ]
		c:     :     [      :        ]
		d: [   : ]     
		"""
		args = CLI(['-m','subset','--no-strand-specific-matching','-s',''])
		
		fusion_1 = Fusion("chr1","chr2",15000,27500,None,None,"+","+","Experiment")
		fusion_2 = Fusion("chr1","chr2",15000,25500,None,None,"+","+","Experiment")
		
		experiment = FusionDetectionExperiment("Experiment_1")
		
		experiment.add_fusion(fusion_1)
		experiment.add_fusion(fusion_2)
		
		self.assertEqual(len(experiment), 2)
		
		genes = ParseBED("tests/data/test_FusionDetectionExperiment.TestFusionDetectionExperiment.test_11.bed","hg18", 200000)
		
		experiment.annotate_genes(genes)
		
		for fusion in experiment:
			if(fusion.get_right_break_position() == 30000):
				self.assertEqual(len(fusion.annotated_genes_right), 3)
				self.assertTrue("NM_00002A" in [str(gene_name) for gene_name in fusion.annotated_genes_right])
				self.assertTrue("NM_00002B" in [str(gene_name) for gene_name in fusion.annotated_genes_right])
				self.assertTrue("NM_00002C" in [str(gene_name) for gene_name in fusion.annotated_genes_right])
			if(fusion.get_right_break_position() == 25000):
				self.assertEqual(len(fusion.annotated_genes_right), 3)
				self.assertTrue("NM_00002A" in [str(gene_name) for gene_name in fusion.annotated_genes_right])
				self.assertTrue("NM_00002B" in [str(gene_name) for gene_name in fusion.annotated_genes_right])
				self.assertTrue("NM_00002D" in [str(gene_name) for gene_name in fusion.annotated_genes_right])
		
		experiment.remove_duplicates(args)
		
		self.assertEqual(len(experiment), 2)
예제 #15
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	def test_09(self):
		"""
		Check the duplication removal - 2 fusions where both are missing
		annotations -> bothshould be lost because it isn't gene
		spanning
		"""
		args = CLI(['-m','subset','--no-strand-specific-matching','-s',''])
		
		fusion_1 = Fusion("chr1","chr2",15000,20000,None,None,"+","+","Experiment")
		fusion_2 = Fusion("chr1","chr2",15000,20000,None,None,"+","+","Experiment")
		
		experiment = FusionDetectionExperiment("Experiment_1")
		experiment.add_fusion(fusion_1)
		experiment.add_fusion(fusion_2)
		
		self.assertEqual(len(experiment), 2)
		
		genes = ParseBED("tests/data/test_FusionDetectionExperiment.TestFusionDetectionExperiment.test_09.bed","hg18", 200000)
		experiment.annotate_genes(genes)
		experiment.remove_duplicates(args)
		
		self.assertEqual(len(experiment), 0)
예제 #16
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	def test_04(self):
		"""
		Check the duplication removal - simple test; 2 identical fusions but checking presevation of the gene names from different annotations
		"""
		args = CLI(['-m','subset','--no-strand-specific-matching','-s',''])
		
		gene_1_hg18 = Gene("gene_1", False)
		gene_1_hg19 = Gene("gene_1", False)
		
		gene_2_hg18 = Gene("gene_2", False)
		gene_2_hg19 = Gene("gene_2", False)
		
		fusion_hg18 = Fusion("chr1","chr2",15000,20000,"+","+","Experiment","",True)
		fusion_hg19 = Fusion("chr1","chr2",15500,20500,"+","+","Experiment","",True)
		
		fusion_hg18.annotate_genes_left([gene_1_hg18])
		fusion_hg19.annotate_genes_left([gene_1_hg19])
		
		fusion_hg18.annotate_genes_right([gene_2_hg18])
		fusion_hg19.annotate_genes_right([gene_2_hg19])
		
		experiment = FusionDetectionExperiment("Experiment_1")
		experiment.genes_spanning_left_junction = [True]
		experiment.genes_spanning_right_junction = [True]
		
		experiment.add_fusion(fusion_hg18)
		experiment.add_fusion(fusion_hg19)
		
		self.assertEqual(len(experiment), 2)
		
		experiment.remove_duplicates(args)
		
		self.assertEqual(len(experiment), 1)
		
		for fusion in experiment:
			self.assertEqual(len(fusion.annotated_genes_left), 2)
	def test_04(self):
		"""
		Check the duplication removal - simple test; 2 identical fusions but checking presevation of the gene names from different annotations
		"""
		args = CLI(['-m','subset','--no-strand-specific-matching','-s',''])
		
		gene_1_hg18 = Gene("gene_1", False)
		gene_1_hg19 = Gene("gene_1", False)
		
		gene_2_hg18 = Gene("gene_2", False)
		gene_2_hg19 = Gene("gene_2", False)
		
		fusion_hg18 = Fusion("chr1","chr2",15000,20000,"+","+","Experiment","",True)
		fusion_hg19 = Fusion("chr1","chr2",15500,20500,"+","+","Experiment","",True)
		
		fusion_hg18.annotate_genes_left([gene_1_hg18])
		fusion_hg19.annotate_genes_left([gene_1_hg19])
		
		fusion_hg18.annotate_genes_right([gene_2_hg18])
		fusion_hg19.annotate_genes_right([gene_2_hg19])
		
		experiment = FusionDetectionExperiment("Experiment_1")
		experiment.genes_spanning_left_junction = [True]
		experiment.genes_spanning_right_junction = [True]
		
		experiment.add_fusion(fusion_hg18)
		experiment.add_fusion(fusion_hg19)
		
		self.assertEqual(len(experiment), 2)
		
		experiment.remove_duplicates(args)
		
		self.assertEqual(len(experiment), 1)
		
		for fusion in experiment:
			self.assertEqual(len(fusion.annotated_genes_left), 2)
예제 #18
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    def test_04(self):
        """
#1:
        -->                     <--
        break1                    break2
        |                         |
[ --- Gene A --- ]        [ --- Gene B --- ]

#2:
      <--                         -->
        break1                    break2
        |                         |
[ --- Gene A --- ]        [ --- Gene B --- ]

Ensure the strand of the merged fusion is not set!
"""
        gene_A = Gene("A", False)
        gene_B = Gene("B", False)

        genes = GeneAnnotation("hg19")
        genes.add_annotation(gene_A, "1", 10000, 20000)
        genes.add_annotation(gene_B, "1", 80000, 90000)

        fusion_1 = Fusion("chr1", "chr1", 15000, 85000, "+", "-",
                          "Experiment_1", 3, True)
        fusion_2 = Fusion("chr1", "chr1", 15000, 85000, "-", "+",
                          "Experiment_2", 4, True)

        experiment_1 = FusionDetectionExperiment("Experiment_1")
        experiment_1.add_fusion(fusion_1)
        experiment_1.annotate_genes(genes)

        experiment_2 = FusionDetectionExperiment("Experiment_2")
        experiment_2.add_fusion(fusion_2)
        experiment_2.annotate_genes(genes)

        args = CLI(
            ['-f', 'summary', '--no-strand-specific-matching', '-s', ''])
        overlapping_complex = OverlapComplex()
        overlapping_complex.add_experiment(experiment_1)
        overlapping_complex.add_experiment(experiment_2)
        overlap = overlapping_complex.overlay_fusions(True, False, args)

        self.assertEqual(overlap[0][0].left_strand, None)
        self.assertEqual(overlap[0][0].right_strand, None)

        args = CLI(
            ['-f', 'summary', '--no-strand-specific-matching', '-s', ''])
        overlapping_complex = OverlapComplex()
        overlapping_complex.add_experiment(experiment_1)
        overlapping_complex.add_experiment(experiment_1)
        overlap = overlapping_complex.overlay_fusions(True, False, args)

        self.assertNotEqual(overlap[0][0].left_strand, None)
        self.assertNotEqual(overlap[0][0].right_strand, None)

        args = CLI(
            ['-f', 'summary', '--no-strand-specific-matching', '-s', ''])
        overlapping_complex = OverlapComplex()
        overlapping_complex.add_experiment(experiment_2)
        overlapping_complex.add_experiment(experiment_2)
        overlap = overlapping_complex.overlay_fusions(True, False, args)

        self.assertNotEqual(overlap[0][0].left_strand, None)
        self.assertNotEqual(overlap[0][0].right_strand, None)
	def test_02(self):
		"""
#1:
        break1                    break2
        |                         |
[ --- Gene A --- ]        [ --- Gene B --- ]

#2:
        break1                    break2
        |                         |
[ --- Gene B --- ]        [ --- Gene A --- ]
		"""
		
		args_off = CLI(['-f','summary','-s',''])
		
		gene_A = Gene("A", False)
		gene_B = Gene("B", False)
		
		genes = GeneAnnotation("hg19")
		genes.add_annotation(gene_A,"1",10000,20000)
		genes.add_annotation(gene_B,"1",80000,90000)
		
		fusion_1 = Fusion("chr1","chr1",15000,85000,"+","+","Experiment_1","1",True)
		experiment_1 = FusionDetectionExperiment("Experiment_1")
		experiment_1.add_fusion(fusion_1)
		experiment_1.annotate_genes(genes)
		
		fusion_2 = Fusion("chr1","chr1",85000,15000,"+","+","Experiment_2","2",True)
		experiment_2 = FusionDetectionExperiment("Experiment_2")
		experiment_2.add_fusion(fusion_2)
		experiment_2.annotate_genes(genes)
		
		self.assertEqual(len(fusion_1.annotated_genes_left) , 1)
		self.assertEqual(len(fusion_2.annotated_genes_left) , 1)
		
		self.assertEqual( fusion_1.acceptor_donor_direction , AD_DIRECTION_FORWARD )
		self.assertEqual( fusion_2.acceptor_donor_direction , AD_DIRECTION_REVERSE )
		
		# -> & <- = unknown
		overlapping_complex = OverlapComplex()
		overlapping_complex.add_experiment(experiment_1)
		overlapping_complex.add_experiment(experiment_2)
		overlap = overlapping_complex.overlay_fusions(True,False,args_off)
		
		self.assertEqual(len(overlap[0]), 1)
		self.assertEqual(overlap[0][0].acceptor_donor_direction, None)
		
		# <- & -> = unknown
		overlapping_complex = OverlapComplex()
		overlapping_complex.add_experiment(experiment_2)
		overlapping_complex.add_experiment(experiment_1)
		overlap = overlapping_complex.overlay_fusions(True,False,args_off)
		
		self.assertEqual(len(overlap[0]), 1)
		self.assertEqual(overlap[0][0].acceptor_donor_direction, None)
		
		# -> & -> = ->
		overlapping_complex = OverlapComplex()
		overlapping_complex.add_experiment(experiment_1)
		overlapping_complex.add_experiment(experiment_1)
		overlap = overlapping_complex.overlay_fusions(True,False,args_off)
		
		self.assertEqual(len(overlap[0]), 1)
		self.assertEqual(overlap[0][0].acceptor_donor_direction, AD_DIRECTION_FORWARD )
		
		# <- & <- = <-
		overlapping_complex = OverlapComplex()
		overlapping_complex.add_experiment(experiment_2)
		overlapping_complex.add_experiment(experiment_2)
		overlap = overlapping_complex.overlay_fusions(True,False,args_off)
		
		self.assertEqual(len(overlap[0]), 1)
		self.assertEqual(overlap[0][0].acceptor_donor_direction, AD_DIRECTION_REVERSE )
예제 #20
0
	def test_13(self):
		"""
		Crazy stuff:
		
		{a} = 1 , 2
		{b} = 1 , 3
		
		   | f1 | f2 | f3 |
		---+----+----+----+
		f1 | *  | 1  | 2  |
		---+----+----+----+
		f2 | 1  | *  |    |
		---+----+----+----+
		f3 | 2  |    | *  |
		---+----+----+----+
		
		1 = [a,b,c]
		2 = [a,c]
		3 = [a,b]
		
		>> Try in all possible orders
		
				Break {1}:
		                  |
		Break {2}:        :
		           |      :
		Break {3}: :      :
		           :      :    | 
		a: [       :      :    :     ]
		b:         :  [   :    :     ]
		c: [       :      :  ] :
		
		"""
		args = CLI(['-m','subset','--no-strand-specific-matching','-s',''])
		
		fusion_1_exp_1 = Fusion("chr1","chr2",15000,70000,"+","+","Experiment_1","",True)
		fusion_2_exp_1 = Fusion("chr1","chr2",15000,80000,"+","+","Experiment_1","",True)
		fusion_3_exp_1 = Fusion("chr1","chr2",15000,60000,"+","+","Experiment_1","",True)
		
		fusion_1_exp_2 = Fusion("chr1","chr2",15000,70000,"+","+","Experiment_2","",True)
		fusion_2_exp_2 = Fusion("chr1","chr2",15000,80000,"+","+","Experiment_2","",True)
		fusion_3_exp_2 = Fusion("chr1","chr2",15000,60000,"+","+","Experiment_2","",True)
		
		fusion_1_exp_3 = Fusion("chr1","chr2",15000,70000,"+","+","Experiment_3","",True)
		fusion_2_exp_3 = Fusion("chr1","chr2",15000,80000,"+","+","Experiment_3","",True)
		fusion_3_exp_3 = Fusion("chr1","chr2",15000,60000,"+","+","Experiment_3","",True)
		
		fusion_1_exp_4 = Fusion("chr1","chr2",15000,70000,"+","+","Experiment_4","",True)
		fusion_2_exp_4 = Fusion("chr1","chr2",15000,80000,"+","+","Experiment_4","",True)
		fusion_3_exp_4 = Fusion("chr1","chr2",15000,60000,"+","+","Experiment_4","",True)
		
		fusion_1_exp_5 = Fusion("chr1","chr2",15000,70000,"+","+","Experiment_5","",True)
		fusion_2_exp_5 = Fusion("chr1","chr2",15000,80000,"+","+","Experiment_5","",True)
		fusion_3_exp_5 = Fusion("chr1","chr2",15000,60000,"+","+","Experiment_5","",True)
		
		fusion_1_exp_6 = Fusion("chr1","chr2",15000,70000,"+","+","Experiment_6","",True)
		fusion_2_exp_6 = Fusion("chr1","chr2",15000,80000,"+","+","Experiment_6","",True)
		fusion_3_exp_6 = Fusion("chr1","chr2",15000,60000,"+","+","Experiment_6","",True)
		
		experiment_1 = FusionDetectionExperiment("Experiment_1")
		experiment_2 = FusionDetectionExperiment("Experiment_2")
		experiment_3 = FusionDetectionExperiment("Experiment_3")
		experiment_4 = FusionDetectionExperiment("Experiment_4")
		experiment_5 = FusionDetectionExperiment("Experiment_5")
		experiment_6 = FusionDetectionExperiment("Experiment_6")
		
		experiment_1.add_fusion(fusion_1_exp_1)
		experiment_1.add_fusion(fusion_2_exp_1)
		experiment_1.add_fusion(fusion_3_exp_1)
		
		experiment_2.add_fusion(fusion_1_exp_2)
		experiment_2.add_fusion(fusion_3_exp_2)
		experiment_2.add_fusion(fusion_2_exp_2)
		
		experiment_3.add_fusion(fusion_2_exp_3)
		experiment_3.add_fusion(fusion_1_exp_3)
		experiment_3.add_fusion(fusion_3_exp_3)
		
		experiment_4.add_fusion(fusion_2_exp_4)
		experiment_4.add_fusion(fusion_3_exp_4)
		experiment_4.add_fusion(fusion_1_exp_4)
		
		experiment_5.add_fusion(fusion_3_exp_5)
		experiment_5.add_fusion(fusion_1_exp_5)
		experiment_5.add_fusion(fusion_2_exp_5)
		
		experiment_6.add_fusion(fusion_3_exp_6)
		experiment_6.add_fusion(fusion_2_exp_6)
		experiment_6.add_fusion(fusion_1_exp_6)
		
		self.assertEqual(len(experiment_1), 3)
		self.assertEqual(len(experiment_2), 3)
		self.assertEqual(len(experiment_3), 3)
		self.assertEqual(len(experiment_4), 3)
		self.assertEqual(len(experiment_5), 3)
		self.assertEqual(len(experiment_6), 3)
		
		genes = ParseBED("tests/data/test_FusionDetectionExperiment.TestFusionDetectionExperiment.test_13.bed","hg18", 200000)
		
		experiment_1.annotate_genes(genes)
		experiment_2.annotate_genes(genes)
		experiment_3.annotate_genes(genes)
		experiment_4.annotate_genes(genes)
		experiment_5.annotate_genes(genes)
		experiment_6.annotate_genes(genes)
		
		experiment_1.remove_duplicates(args)
		experiment_2.remove_duplicates(args)
		experiment_3.remove_duplicates(args)
		experiment_4.remove_duplicates(args)
		experiment_5.remove_duplicates(args)
		experiment_6.remove_duplicates(args)
		
		# Removing duplicates:
		# 
		# order 1:
		# -> [A,B]   <-> [A,C]   = [A,B]  & [A,C] & [A,B,C]
		# -> [A,B]   <-> [A,B,C] = [A,B*] & [A,C]
		# -> [A,B*]  <-> [A,C]   = [A,B*] & [A,C]
		
		# order 2:
		# -> [A,B]   <-> [A,B,C] = [A,B*] & [A,C]
		# -> [A,B*]  <-> [A,C]   = [A,B*] & [A,C]
		
		# order 3:
		# -> [A,C]   <-> [A,B]   = [A,C]  & [A,B] & [A,B,C]
		# -> [A,C]   <-> [A,B,C] = [A,C*] & [A,B]
		# -> [A,C*]  <-> [A,B]   = [A,C*] & [A,B]
		
		# order 4:
		# -> [A,C]   <-> [A,B,C] = [A,C*] & [A,B]
		# -> [A,C*]  <-> [A,B]   = [A,C*] & [A,B]
		
		# order 5:
		# -> [A,B,C] <-> [A,B]   = [A,B*] & [A,C]
		# -> [A,B*]  <-> [A,C]   = [A,B*] & [A,C]
		
		# order 6:
		# -> [A,B,C] <-> [A,C]   = [A,C*] & [A,B]
		# -> [A,C*]  <-> [A,B]   = [A,C*] & [A,B]
		
		self.assertEqual(len(experiment_1), 2)
		self.assertEqual(len(experiment_2), 2)
		self.assertEqual(len(experiment_3), 2)
		self.assertEqual(len(experiment_4), 2)
		self.assertEqual(len(experiment_5), 2)
		self.assertEqual(len(experiment_6), 2)
	def test_13(self):
		"""
		Crazy stuff:
		
		{a} = 1 , 2
		{b} = 1 , 3
		
		   | f1 | f2 | f3 |
		---+----+----+----+
		f1 | *  | 1  | 2  |
		---+----+----+----+
		f2 | 1  | *  |    |
		---+----+----+----+
		f3 | 2  |    | *  |
		---+----+----+----+
		
		1 = [a,b,c]
		2 = [a,c]
		3 = [a,b]
		
		>> Try in all possible orders
		
				Break {1}:
		                  |
		Break {2}:        :
		           |      :
		Break {3}: :      :
		           :      :    | 
		a: [       :      :    :     ]
		b:         :  [   :    :     ]
		c: [       :      :  ] :
		
		"""
		args = CLI(['-m','subset','--no-strand-specific-matching','-s',''])
		
		fusion_1_exp_1 = Fusion("chr1","chr2",15000,70000,"+","+","Experiment_1","",True)
		fusion_2_exp_1 = Fusion("chr1","chr2",15000,80000,"+","+","Experiment_1","",True)
		fusion_3_exp_1 = Fusion("chr1","chr2",15000,60000,"+","+","Experiment_1","",True)
		
		fusion_1_exp_2 = Fusion("chr1","chr2",15000,70000,"+","+","Experiment_2","",True)
		fusion_2_exp_2 = Fusion("chr1","chr2",15000,80000,"+","+","Experiment_2","",True)
		fusion_3_exp_2 = Fusion("chr1","chr2",15000,60000,"+","+","Experiment_2","",True)
		
		fusion_1_exp_3 = Fusion("chr1","chr2",15000,70000,"+","+","Experiment_3","",True)
		fusion_2_exp_3 = Fusion("chr1","chr2",15000,80000,"+","+","Experiment_3","",True)
		fusion_3_exp_3 = Fusion("chr1","chr2",15000,60000,"+","+","Experiment_3","",True)
		
		fusion_1_exp_4 = Fusion("chr1","chr2",15000,70000,"+","+","Experiment_4","",True)
		fusion_2_exp_4 = Fusion("chr1","chr2",15000,80000,"+","+","Experiment_4","",True)
		fusion_3_exp_4 = Fusion("chr1","chr2",15000,60000,"+","+","Experiment_4","",True)
		
		fusion_1_exp_5 = Fusion("chr1","chr2",15000,70000,"+","+","Experiment_5","",True)
		fusion_2_exp_5 = Fusion("chr1","chr2",15000,80000,"+","+","Experiment_5","",True)
		fusion_3_exp_5 = Fusion("chr1","chr2",15000,60000,"+","+","Experiment_5","",True)
		
		fusion_1_exp_6 = Fusion("chr1","chr2",15000,70000,"+","+","Experiment_6","",True)
		fusion_2_exp_6 = Fusion("chr1","chr2",15000,80000,"+","+","Experiment_6","",True)
		fusion_3_exp_6 = Fusion("chr1","chr2",15000,60000,"+","+","Experiment_6","",True)
		
		experiment_1 = FusionDetectionExperiment("Experiment_1")
		experiment_2 = FusionDetectionExperiment("Experiment_2")
		experiment_3 = FusionDetectionExperiment("Experiment_3")
		experiment_4 = FusionDetectionExperiment("Experiment_4")
		experiment_5 = FusionDetectionExperiment("Experiment_5")
		experiment_6 = FusionDetectionExperiment("Experiment_6")
		
		experiment_1.add_fusion(fusion_1_exp_1)
		experiment_1.add_fusion(fusion_2_exp_1)
		experiment_1.add_fusion(fusion_3_exp_1)
		
		experiment_2.add_fusion(fusion_1_exp_2)
		experiment_2.add_fusion(fusion_3_exp_2)
		experiment_2.add_fusion(fusion_2_exp_2)
		
		experiment_3.add_fusion(fusion_2_exp_3)
		experiment_3.add_fusion(fusion_1_exp_3)
		experiment_3.add_fusion(fusion_3_exp_3)
		
		experiment_4.add_fusion(fusion_2_exp_4)
		experiment_4.add_fusion(fusion_3_exp_4)
		experiment_4.add_fusion(fusion_1_exp_4)
		
		experiment_5.add_fusion(fusion_3_exp_5)
		experiment_5.add_fusion(fusion_1_exp_5)
		experiment_5.add_fusion(fusion_2_exp_5)
		
		experiment_6.add_fusion(fusion_3_exp_6)
		experiment_6.add_fusion(fusion_2_exp_6)
		experiment_6.add_fusion(fusion_1_exp_6)
		
		self.assertEqual(len(experiment_1), 3)
		self.assertEqual(len(experiment_2), 3)
		self.assertEqual(len(experiment_3), 3)
		self.assertEqual(len(experiment_4), 3)
		self.assertEqual(len(experiment_5), 3)
		self.assertEqual(len(experiment_6), 3)
		
		genes = ParseBED("tests/data/test_FusionDetectionExperiment.TestFusionDetectionExperiment.test_13.bed","hg18", 200000)
		
		experiment_1.annotate_genes(genes)
		experiment_2.annotate_genes(genes)
		experiment_3.annotate_genes(genes)
		experiment_4.annotate_genes(genes)
		experiment_5.annotate_genes(genes)
		experiment_6.annotate_genes(genes)
		
		experiment_1.remove_duplicates(args)
		experiment_2.remove_duplicates(args)
		experiment_3.remove_duplicates(args)
		experiment_4.remove_duplicates(args)
		experiment_5.remove_duplicates(args)
		experiment_6.remove_duplicates(args)
		
		# Removing duplicates:
		# 
		# order 1:
		# -> [A,B]   <-> [A,C]   = [A,B]  & [A,C] & [A,B,C]
		# -> [A,B]   <-> [A,B,C] = [A,B*] & [A,C]
		# -> [A,B*]  <-> [A,C]   = [A,B*] & [A,C]
		
		# order 2:
		# -> [A,B]   <-> [A,B,C] = [A,B*] & [A,C]
		# -> [A,B*]  <-> [A,C]   = [A,B*] & [A,C]
		
		# order 3:
		# -> [A,C]   <-> [A,B]   = [A,C]  & [A,B] & [A,B,C]
		# -> [A,C]   <-> [A,B,C] = [A,C*] & [A,B]
		# -> [A,C*]  <-> [A,B]   = [A,C*] & [A,B]
		
		# order 4:
		# -> [A,C]   <-> [A,B,C] = [A,C*] & [A,B]
		# -> [A,C*]  <-> [A,B]   = [A,C*] & [A,B]
		
		# order 5:
		# -> [A,B,C] <-> [A,B]   = [A,B*] & [A,C]
		# -> [A,B*]  <-> [A,C]   = [A,B*] & [A,C]
		
		# order 6:
		# -> [A,B,C] <-> [A,C]   = [A,C*] & [A,B]
		# -> [A,C*]  <-> [A,B]   = [A,C*] & [A,B]
		
		self.assertEqual(len(experiment_1), 2)
		self.assertEqual(len(experiment_2), 2)
		self.assertEqual(len(experiment_3), 2)
		self.assertEqual(len(experiment_4), 2)
		self.assertEqual(len(experiment_5), 2)
		self.assertEqual(len(experiment_6), 2)