def writeIndexes(self):
numIndexElements = int(math.ceil(1.0 * self._chrSize / CompBinManager.getIndexBinSize()))
self._leftIndexFile = OutputFile(self._path, 'leftIndex', numIndexElements, allowAppend=False)
self._rightIndexFile = OutputFile(self._path, 'rightIndex', numIndexElements, allowAppend=False)
if self._startFile:
lefts = self._startFile.getContents()
else:
lefts = np.r_[0, self._endFile.getContents()[:-1]]
if self._endFile:
rights = self._endFile.getContents()
if not self._startFile:
rights = rights[1:]
else:
rights = self._startFile.getContents() + 1
bin_i = 0
i = 0
for i, right in enumerate(rights):
while right > (bin_i) * CompBinManager.getIndexBinSize():
self._leftIndexFile.write(i)
bin_i += 1
bin_j = 0
j = 0
for j, left in enumerate(lefts):
while left >= (bin_j+1) * CompBinManager.getIndexBinSize():
self._rightIndexFile.write(j)
bin_j += 1
self._fillRestOfIndexFile(bin_i, i+1, self._leftIndexFile)
self._fillRestOfIndexFile(bin_j, j+1, self._rightIndexFile)
def testIsCompBin(self):
self.assertTrue(
CompBinManager.isCompBin(
GenomeRegion('TestGenome', 'chr21', 0, 100)))
self.assertTrue(
CompBinManager.isCompBin(
GenomeRegion('TestGenome', 'chr21', 200, 300)))
self.assertTrue(
CompBinManager.isCompBin(
GenomeRegion('TestGenome', 'chr21', 46944300, 46944323)))
self.assertFalse(
CompBinManager.isCompBin(GenomeRegion('TestGenome', 'chr21', 0,
40)))
self.assertFalse(
CompBinManager.isCompBin(
GenomeRegion('TestGenome', 'chr21', 10, 100)))
self.assertFalse(
CompBinManager.isCompBin(
GenomeRegion('TestGenome', 'chr21', 10, 200)))
self.assertFalse(
CompBinManager.isCompBin(
GenomeRegion('TestGenome', 'chr21', 100, 300)))
self.assertFalse(
CompBinManager.isCompBin(
GenomeRegion('TestGenome', 'chr21', 46944300, 46944322)))
self.assertFalse(
CompBinManager.isCompBin(
GenomeRegion('TestGenome', 'chr21', 46944300, 46944324)))
def testGetNumOfBins(self):
self.assertEqual(
0, CompBinManager.getNumOfBins(GenomeRegion('hg18', 'chr1', 0, 0)))
self.assertEqual(
1,
CompBinManager.getNumOfBins(GenomeRegion('hg18', 'chr1', 0, 100)))
self.assertEqual(
2,
CompBinManager.getNumOfBins(GenomeRegion('hg18', 'chr1', 200,
400)))
self.assertEqual(
4,
CompBinManager.getNumOfBins(GenomeRegion('hg18', 'chr1', 67, 314)))
def testIsCompBin(self):
self.assertTrue(CompBinManager.isCompBin( GenomeRegion('TestGenome', 'chr21', 0, 100) ))
self.assertTrue(CompBinManager.isCompBin( GenomeRegion('TestGenome', 'chr21', 200, 300) ))
self.assertTrue(CompBinManager.isCompBin( GenomeRegion('TestGenome', 'chr21', 46944300, 46944323) ))
self.assertFalse(CompBinManager.isCompBin( GenomeRegion('TestGenome', 'chr21', 0, 40) ))
self.assertFalse(CompBinManager.isCompBin( GenomeRegion('TestGenome', 'chr21', 10, 100) ))
self.assertFalse(CompBinManager.isCompBin( GenomeRegion('TestGenome', 'chr21', 10, 200) ))
self.assertFalse(CompBinManager.isCompBin( GenomeRegion('TestGenome', 'chr21', 100, 300) ))
self.assertFalse(CompBinManager.isCompBin( GenomeRegion('TestGenome', 'chr21', 46944300, 46944322) ))
self.assertFalse(CompBinManager.isCompBin( GenomeRegion('TestGenome', 'chr21', 46944300, 46944324) ))
def _assertSplitUserBin(self, compBins, start, end):
region = GenomeRegion('hg18', 'chr1', start, end)
compBinRegions = [
GenomeRegion('hg18', 'chr1', elStart, elEnd)
for elStart, elEnd in compBins
]
AssertList(compBinRegions, CompBinManager.splitUserBin(region),
self.assertEqual)
def loadTrackView(trackData, region, borderHandling, allowOverlaps, trackName=[]):
"""
trackData : see TrackSource.getTrackData {'id' : smartmemmap}
region : see GenomeRegion
"""
#brShelve = BoundingRegionShelve(region.genome, trackName, allowOverlaps)
brShelve = trackData.boundingRegionShelve
brInfo = brShelve.getBoundingRegionInfo(region) if brShelve is not None else None
extraArrayNames = [arrayName for arrayName in trackData if arrayName not in \
RESERVED_PREFIXES.keys() + ['leftIndex', 'rightIndex']]
reservedArrays = [TrackViewLoader._getArray(trackData, arrayName, brInfo) for arrayName in RESERVED_PREFIXES]
extraArrays = [TrackViewLoader._getArray(trackData, arrayName, brInfo) for arrayName in extraArrayNames]
trackFormat = TrackFormat( *(reservedArrays + [OrderedDict(zip(extraArrayNames, extraArrays))]) )
if trackFormat.reprIsDense():
if brInfo is None:
leftIndex = region.start
rightIndex = region.end
else:
leftIndex = region.start - brInfo.start
rightIndex = region.end - brInfo.start
else:
leftBin = CompBinManager.getBinNumber(region.start)
rightBin = CompBinManager.getBinNumber(region.end-1)
#leftBin = region.start/COMP_BIN_SIZE
#rightBin = (region.end-1)/COMP_BIN_SIZE
if trackData.get('leftIndex') is None or trackData.get('rightIndex') is None:
raise IOError('Preprocessed track not found. TrackData: ' + ', '.join(trackData.keys()))
leftIndex = TrackViewLoader._getArray(trackData, 'leftIndex', brInfo, leftBin)
rightIndex = TrackViewLoader._getArray(trackData, 'rightIndex', brInfo, rightBin)
slicedReservedArrays = [(array[leftIndex:rightIndex] if array is not None else None) for array in reservedArrays]
slicedExtraArrays = [(array[leftIndex:rightIndex] if array is not None else None) for array in extraArrays]
argList = [region] + slicedReservedArrays + [borderHandling, allowOverlaps] + [OrderedDict(zip(extraArrayNames, slicedExtraArrays))]
tv = TrackView( *(argList) )
if not trackFormat.reprIsDense():
tv.sliceElementsAccordingToGenomeAnchor()
#tv._doScatteredSlicing()
return tv
def writeIndexes(self):
numIndexElements = int(
math.ceil(1.0 * self._chrSize / CompBinManager.getIndexBinSize()))
self._leftIndexFile = OutputFile(self._path,
'leftIndex',
numIndexElements,
allowAppend=False)
self._rightIndexFile = OutputFile(self._path,
'rightIndex',
numIndexElements,
allowAppend=False)
if self._startFile:
lefts = self._startFile.getContents()
else:
lefts = np.r_[0, self._endFile.getContents()[:-1]]
if self._endFile:
rights = self._endFile.getContents()
if not self._startFile:
rights = rights[1:]
else:
rights = self._startFile.getContents() + 1
bin_i = 0
i = 0
for i, right in enumerate(rights):
while right > (bin_i) * CompBinManager.getIndexBinSize():
self._leftIndexFile.write(i)
bin_i += 1
bin_j = 0
j = 0
for j, left in enumerate(lefts):
while left >= (bin_j + 1) * CompBinManager.getIndexBinSize():
self._rightIndexFile.write(j)
bin_j += 1
self._fillRestOfIndexFile(bin_i, i + 1, self._leftIndexFile)
self._fillRestOfIndexFile(bin_j, j + 1, self._rightIndexFile)
def createDirPath(trackName, genome, chr=None, allowOverlaps=False, basePath=Config.PROCESSED_DATA_PATH):
"""
>>> createDirPath(['trackname'],'genome','chr1')
'/100000/noOverlaps/genome/trackname/chr1'
"""
from gtrackcore.util.CompBinManager import CompBinManager
if len(trackName)>0 and trackName[0] == 'redirect':
genome = trackName[1]
chr = trackName[2]
#trackName[3] is description
trackName = trackName[4:]
#print [basePath, str(CompBinManager.getIndexBinSize()), ('withOverlaps' if allowOverlaps else 'noOverlaps'), genome] +\
# list(trackName) + ([chr] if chr is not None else [])
return os.sep.join( [basePath, str(CompBinManager.getIndexBinSize()), ('withOverlaps' if allowOverlaps else 'noOverlaps'), genome] +\
list(trackName) + ([chr] if chr is not None else []) )
def createDirPath(trackName,
genome,
chr=None,
allowOverlaps=False,
basePath=Config.PROCESSED_DATA_PATH):
"""
>>> createDirPath(['trackname'],'genome','chr1')
'/100000/noOverlaps/genome/trackname/chr1'
"""
from gtrackcore.util.CompBinManager import CompBinManager
if len(trackName) > 0 and trackName[0] == 'redirect':
genome = trackName[1]
chr = trackName[2]
#trackName[3] is description
trackName = trackName[4:]
#print [basePath, str(CompBinManager.getIndexBinSize()), ('withOverlaps' if allowOverlaps else 'noOverlaps'), genome] +\
# list(trackName) + ([chr] if chr is not None else [])
return os.sep.join( [basePath, str(CompBinManager.getIndexBinSize()), ('withOverlaps' if allowOverlaps else 'noOverlaps'), genome] +\
list(trackName) + ([chr] if chr is not None else []) )
def storeBoundingRegions(self, boundingRegionTuples, genomeElementChrList, sparse):
assert sparse in [False, True]
tempContents = OrderedDict()
genomeElementChrs = set(genomeElementChrList)
lastRegion = None
chrStartIdxs = OrderedDict()
chrEndIdxs = OrderedDict()
totElCount = 0
totBinCount = 0
for br in boundingRegionTuples:
if lastRegion is None or br.region.chr != lastRegion.chr:
if br.region.chr in tempContents:
raise InvalidFormatError("Error: bounding region (%s) is not grouped with previous bounding regions of the same chromosome (sequence)." % br.region)
lastRegion = None
tempContents[br.region.chr] = OrderedDict()
if sparse:
chrStartIdxs[br.region.chr] = totElCount
else:
if br.region < lastRegion:
raise InvalidFormatError("Error: bounding regions in the same chromosome (sequence) are unsorted: %s > %s." % (lastRegion, br.region))
if lastRegion.overlaps(br.region):
raise InvalidFormatError("Error: bounding regions '%s' and '%s' overlap." % (lastRegion, br.region))
if lastRegion.end == br.region.start:
raise InvalidFormatError("Error: bounding regions '%s' and '%s' are adjoining (there is no gap between them)." % (lastRegion, br.region))
if len(br.region) < 1:
raise InvalidFormatError("Error: bounding region '%s' does not have positive length." % br.region)
if not sparse and len(br.region) != br.elCount:
raise InvalidFormatError("Error: track type representation is dense, but the length of bounding region '%s' is not equal to the element count: %s != %s" % (br.region, len(br.region), br.elCount))
startIdx, endIdx = (totElCount, totElCount + br.elCount) if not sparse else (None, None)
totElCount += br.elCount
if sparse:
chrEndIdxs[br.region.chr] = totElCount
tempContents[br.region.chr][br.region.start] = BoundingRegionInfo(br.region.start, br.region.end, startIdx, endIdx, 0, 0)
lastRegion = br.region
if sparse:
totBinCount = 0
for chr in tempContents:
chrLen = GenomeInfo.getChrLen(self._genome, chr)
numBinsInChr = CompBinManager.getNumOfBins(GenomeRegion(start=0, end=chrLen))
for key in tempContents[chr].keys():
startBinIdx = totBinCount
endBinIdx = totBinCount + numBinsInChr
brInfo = tempContents[chr][key]
if chr in genomeElementChrs:
tempContents[chr][key] = BoundingRegionInfo(brInfo.start, brInfo.end, \
chrStartIdxs[chr], chrEndIdxs[chr], \
startBinIdx, endBinIdx)
else:
if chrEndIdxs[chr] - chrStartIdxs[chr] > 0:
raise InvalidFormatError("Error: bounding region '%s' has incorrect element count: %s > 0" % (GenomeRegion(chr=chr, start=brInfo.start, end=brInfo.end), chrEndIdxs[chr] - chrStartIdxs[chr]))
tempContents[chr][key] = BoundingRegionInfo(brInfo.start, brInfo.end, 0, 0, 0, 0)
if chr in genomeElementChrs:
totBinCount += numBinsInChr
if len(genomeElementChrs - set(tempContents.keys())) > 0:
raise InvalidFormatError('Error: some chromosomes (sequences) contains data, but has no bounding regions: %s' % ', '.join(genomeElementChrs - set(tempContents.keys())))
ensurePathExists(self._fn)
for chr in tempContents:
brInfoDict = tempContents[chr]
tempContents[chr] = BrInfoHolder(tuple(brInfoDict.keys()), tuple(brInfoDict.values()))
brShelve = safeshelve.open(self._fn)
brShelve.update(tempContents)
brShelve.close()
while not self.fileExists():
from gtrackcore.application.LogSetup import logMessage
logMessage("Bounding region shelve file '%s' has yet to be created" % self._fn)
import time
time.sleep(0.2)
def testGetBinNumber(self):
self.assertEqual(0, CompBinManager.getBinNumber(0))
self.assertEqual(2, CompBinManager.getBinNumber(200))
self.assertEqual(3, CompBinManager.getBinNumber(314))
def testGetOffset(self):
self.assertEqual(0, CompBinManager.getOffset(0,0))
self.assertEqual(0,CompBinManager.getOffset(200,2))
self.assertEqual(14,CompBinManager.getOffset(314,3))
self.assertEqual(-86,CompBinManager.getOffset(314,4))
def testGetNumOfBins(self):
self.assertEqual(0, CompBinManager.getNumOfBins(GenomeRegion('hg18', 'chr1', 0, 0)))
self.assertEqual(1, CompBinManager.getNumOfBins(GenomeRegion('hg18', 'chr1', 0, 100)))
self.assertEqual(2, CompBinManager.getNumOfBins(GenomeRegion('hg18', 'chr1', 200, 400)))
self.assertEqual(4, CompBinManager.getNumOfBins(GenomeRegion('hg18', 'chr1', 67, 314)))
def testGetPosFromBinNumber(self):
self.assertEqual(0, CompBinManager.getPosFromBinNumber(0))
self.assertEqual(200, CompBinManager.getPosFromBinNumber(2))
self.assertEqual(300, CompBinManager.getPosFromBinNumber(3))
def testGetBinNumber(self):
self.assertEqual(0, CompBinManager.getBinNumber(0))
self.assertEqual(2, CompBinManager.getBinNumber(200))
self.assertEqual(3, CompBinManager.getBinNumber(314))
def _assertSplitUserBin(self, compBins, start, end):
region = GenomeRegion('hg18','chr1', start, end)
compBinRegions = [GenomeRegion('hg18', 'chr1', elStart, elEnd) for elStart, elEnd in compBins]
AssertList(compBinRegions, CompBinManager.splitUserBin(region), self.assertEqual)
def testGetPosFromBinNumber(self):
self.assertEqual(0, CompBinManager.getPosFromBinNumber(0))
self.assertEqual(200, CompBinManager.getPosFromBinNumber(2))
self.assertEqual(300, CompBinManager.getPosFromBinNumber(3))
def storeBoundingRegions(self, boundingRegionTuples, genomeElementChrList,
sparse):
assert sparse in [False, True]
tempContents = OrderedDict()
genomeElementChrs = set(genomeElementChrList)
lastRegion = None
chrStartIdxs = OrderedDict()
chrEndIdxs = OrderedDict()
totElCount = 0
totBinCount = 0
for br in boundingRegionTuples:
if lastRegion is None or br.region.chr != lastRegion.chr:
if br.region.chr in tempContents:
raise InvalidFormatError(
"Error: bounding region (%s) is not grouped with previous bounding regions of the same chromosome (sequence)."
% br.region)
lastRegion = None
tempContents[br.region.chr] = OrderedDict()
if sparse:
chrStartIdxs[br.region.chr] = totElCount
else:
if br.region < lastRegion:
raise InvalidFormatError(
"Error: bounding regions in the same chromosome (sequence) are unsorted: %s > %s."
% (lastRegion, br.region))
if lastRegion.overlaps(br.region):
raise InvalidFormatError(
"Error: bounding regions '%s' and '%s' overlap." %
(lastRegion, br.region))
if lastRegion.end == br.region.start:
raise InvalidFormatError(
"Error: bounding regions '%s' and '%s' are adjoining (there is no gap between them)."
% (lastRegion, br.region))
if len(br.region) < 1:
raise InvalidFormatError(
"Error: bounding region '%s' does not have positive length."
% br.region)
if not sparse and len(br.region) != br.elCount:
raise InvalidFormatError(
"Error: track type representation is dense, but the length of bounding region '%s' is not equal to the element count: %s != %s"
% (br.region, len(br.region), br.elCount))
startIdx, endIdx = (totElCount, totElCount +
br.elCount) if not sparse else (None, None)
totElCount += br.elCount
if sparse:
chrEndIdxs[br.region.chr] = totElCount
tempContents[br.region.chr][br.region.start] = BoundingRegionInfo(
br.region.start, br.region.end, startIdx, endIdx, 0, 0)
lastRegion = br.region
if sparse:
totBinCount = 0
for chr in tempContents:
chrLen = GenomeInfo.getChrLen(self._genome, chr)
numBinsInChr = CompBinManager.getNumOfBins(
GenomeRegion(start=0, end=chrLen))
for key in tempContents[chr].keys():
startBinIdx = totBinCount
endBinIdx = totBinCount + numBinsInChr
brInfo = tempContents[chr][key]
if chr in genomeElementChrs:
tempContents[chr][key] = BoundingRegionInfo(brInfo.start, brInfo.end, \
chrStartIdxs[chr], chrEndIdxs[chr], \
startBinIdx, endBinIdx)
else:
if chrEndIdxs[chr] - chrStartIdxs[chr] > 0:
raise InvalidFormatError(
"Error: bounding region '%s' has incorrect element count: %s > 0"
% (GenomeRegion(chr=chr,
start=brInfo.start,
end=brInfo.end),
chrEndIdxs[chr] - chrStartIdxs[chr]))
tempContents[chr][key] = BoundingRegionInfo(
brInfo.start, brInfo.end, 0, 0, 0, 0)
if chr in genomeElementChrs:
totBinCount += numBinsInChr
if len(genomeElementChrs - set(tempContents.keys())) > 0:
raise InvalidFormatError(
'Error: some chromosomes (sequences) contains data, but has no bounding regions: %s'
% ', '.join(genomeElementChrs - set(tempContents.keys())))
ensurePathExists(self._fn)
for chr in tempContents:
brInfoDict = tempContents[chr]
tempContents[chr] = BrInfoHolder(tuple(brInfoDict.keys()),
tuple(brInfoDict.values()))
brShelve = safeshelve.open(self._fn)
brShelve.update(tempContents)
brShelve.close()
while not self.fileExists():
from gtrackcore.application.LogSetup import logMessage
logMessage(
"Bounding region shelve file '%s' has yet to be created" %
self._fn)
import time
time.sleep(0.2)
def testGetOffset(self):
self.assertEqual(0, CompBinManager.getOffset(0, 0))
self.assertEqual(0, CompBinManager.getOffset(200, 2))
self.assertEqual(14, CompBinManager.getOffset(314, 3))
self.assertEqual(-86, CompBinManager.getOffset(314, 4))
