def combination(log, conf, rs, c, data_repo, results_path, conditions):
cid = c["id"]
ids = c["source/ids"]
files = c["files"]
results_url = data_repo.url(results_path)
try:
# prepare temporary path and files
tmp_path = mkdtemp(prefix = "cnv_combination_")
data_file = os.path.join(tmp_path, "data.tdm")
columns_file = os.path.join(tmp_path, "columns.gmt")
tmp_file = os.path.join(tmp_path, "tmp.tdm")
log.debug("Temporary directory: {}".format(tmp_path))
# join files to combine in a single TDM file
log.info("Joining files ...".format(files[0]))
outpf = FileWriter(data_file)
log.debug("\t{} ...".format(files[0]))
repo, path = rs.from_url(files[0])
local_path = repo.get_local(path)
ref_hdr = tdm.unflatten(local_path, outpf, row_column = "id",
column_and_attr_func = lambda name: unflatten_filtered_names(name, ids[0]))
#outpf.flush()
#ref_hdr = tdm.read_header_names(data_file)
repo.close_local(path)
for i in xrange(1, len(files)):
log.debug("\t{} ...".format(files[i]))
repo, path = rs.from_url(files[i])
local_path = repo.get_local(path)
hdr = tdm.unflatten(local_path, tmp_file, row_column = "id",
column_and_attr_func = lambda name: unflatten_filtered_names(name, ids[i]))
tdm.append(outpf, tmp_file, ref_hdr)
repo.close_local(path)
outpf.close()
# prepare conditions columns file in GMT format
outpf = FileWriter(columns_file)
for cond in conditions:
outpf.write(cond)
outpf.write("\t\t")
outpf.write("\t".join(["_".join((sid, cond)) for sid in ids]))
outpf.write("\n")
outpf.close()
# run gitools-combination with data.tdm
log.info("Running gitools combination ...")
log.debug("\tData: {}".format(data_file))
log.debug("\tColumns: {}".format(columns_file))
gitools_combination_bin = os.path.join(conf["bin_paths.gitools"], "bin", "gitools-combination")
cmd = " ".join([ gitools_combination_bin,
"-N", cid, "-w", tmp_path,
"-d", data_file,
"-c", columns_file,
"-pn", P_VALUE_FIELD,
"-sn n",
"-p 1", "-debug"])
log.debug(cmd)
retcode = subprocess.call(args = cmd, shell = True)
sys.stdout.write("\n")
sys.stdout.flush()
if retcode != 0:
raise Exception("Combination exit code = {}".format(retcode))
# flatten results
log.info("Flattening results into {} ...".format(results_url))
try:
results_local_path = data_repo.create_local(results_path)
tdm.flatten(os.path.join(tmp_path, cid + "-results.tdm.gz"), results_local_path,
None, ["N", "z-score", "p-value"])
data_repo.put_local(results_local_path)
except:
data_repo.close_local(results_local_path)
finally:
shutil.rmtree(tmp_path)
def enrichment(log, conf, rs, data_repo, results_path, data_file, e, ec,
filtered_columns, filtered_columns_new_names):
eid = e["id"]
key = (e["study_id"], e["platform_id"], e["icdo_topography"], e["icdo_morphology"])
# determine the modules file
mod_repo, mod_path = rs.from_url(ec["modules_file"])
mod_local_path = mod_repo.get_local(mod_path)
# oncodrive data file
matrix_repo, matrix_path = rs.from_url(data_file)
matrix_local_path = matrix_repo.get_local(matrix_path)
e["data_file"] = data_file
e["modules_file"] = ec["modules_file"]
results_local_path = None
tmp_path = mkdtemp(prefix = "enrichment_")
log.debug("Temporary directory: {}".format(tmp_path))
valid = True
try:
log.info("Filtering pvalue columns from {} ...".format(data_file))
# filter columns for pvalues
data_local_path = os.path.join(tmp_path, "data.tsv")
rf = of = None
try:
rf = FileReader(matrix_local_path)
of = FileWriter(data_local_path)
row_count = tsv.filter_columns(rf, of,
filtered_columns, filtered_columns_new_names)
finally:
if rf is not None:
rf.close()
if of is not None:
of.close()
if row_count == 0:
log.warn("Oncodrive results are empty: {}".format(matrix_path))
raise EmptyResults
# apply background if necessary
if "population.file" in ec:
pop_url = ec["population.file"]
pop_missing_value = ec.get("population.missing_value", "-")
log.info("Applying background from {} with missing value {} ...".format(pop_url, pop_missing_value))
data2_local_path = os.path.join(tmp_path, "data-filtered.tsv")
pop_repo, pop_path = rs.from_url(pop_url)
pop_local_path = pop_repo.get_local(pop_path)
cmd = " ".join([
conf["bin_paths.python"], conf["bin_paths.matrix_background"],
"--verbose --missing-value", pop_missing_value,
"-o", data2_local_path,
data_local_path, pop_local_path ])
log.debug(cmd)
retcode = subprocess.call(args = cmd, shell = True)
if retcode != 0:
raise Exception("Applying population background for ({}) [{}] failed with code {}".format(", ".join(key), eid, retcode))
pop_repo.close_local(pop_local_path)
data_local_path = data2_local_path
# enrichment results
e["results_file"] = data_repo.url(results_path)
results_local_path = data_repo.create_local(results_path)
log.info("Running enrichment ...")
log.debug("\tData file: {}".format(data_local_path))
log.debug("\tModules file: {}".format(ec["modules_file"]))
gitools_enrichment_bin = os.path.join(conf["bin_paths.gitools"], "bin", "gitools-enrichment")
sb = [ gitools_enrichment_bin,
"-N", eid, "-w", tmp_path, "-p 1",
"-mf tcm", "-m", mod_local_path,
"-df cdm", "-d", data_local_path,
"-t", ec["test"] ]
if "filter" in ec:
sb += ["-b", ec["filter"]]
if ec.get("only_mapped_items", False, dtype=bool):
sb += ["-only-mapped-items"]
#if "population" in ec:
# pop_repo, pop_path = rs.from_url(ec["population"])
# pop_local_path = pop_repo.get_local(pop_path)
# sb += ["-P", pop_local_path]
cmd = " ".join(sb)
log.debug(cmd)
retcode = subprocess.call(args = cmd, shell = True)
sys.stdout.write("\n")
sys.stdout.flush()
if retcode != 0:
raise Exception("Enrichment for ({}) [{}] failed with code {}".format(", ".join(key), eid, retcode))
# flatten results
log.info("Flattening results into {} ...".format(e["results_file"]))
try:
gitools_results = os.path.join(tmp_path, eid + "-results.tdm.gz")
rf = FileReader(gitools_results)
of = FileWriter(results_local_path)
tdm.flatten(rf, of,
{ "column" : str, "row" : str, "N" : int, "observed" : int,
"expected-mean" : float, "expected-stdev" : float, "probability" : float,
"right-p-value" : float, "corrected-right-p-value" : float },
["N", "observed", "expected-mean", "expected-stdev",
"probability", "right-p-value", "corrected-right-p-value"])
finally:
if rf is not None:
rf.close()
if of is not None:
of.close()
# close local paths
data_repo.put_local(results_local_path)
except EmptyResults:
valid = False
except Exception as ex:
log.exception(ex)
if results_local_path is not None:
data_repo.close_local(results_local_path)
valid = False
finally:
shutil.rmtree(tmp_path)
mod_repo.close_local(mod_local_path)
data_repo.close_local(matrix_local_path)
#if "population" in ec:
# pop_repo.close_local(pop_local_path)
return valid