def finalize_project(project):
log = task.logger
config = GlobalConfig(task.conf)
paths = PathsConfig(config)
project_id = project["id"]
log.info("--- [{0}] --------------------------------------------".format(project_id))
log.info("Cleaning project ...")
projres = ProjectResults(project)
projres.clean(config)
log.info("Saving project configuration ...")
projres.save_def()
if config.results.use_storage:
log.info("Compressing the database ...")
db_path = os.path.join(projres.path, "project.db")
temp_path = tempfile.mkdtemp(prefix="intogen-mutations-{}-".format(project_id))
compressed_db_path = os.path.join(temp_path, "project.db.gz")
try:
cmd = "gzip -c {} >{}".format(db_path, compressed_db_path)
log.debug("> {}".format(cmd))
retcode = subprocess.call(cmd, shell=True)
if retcode == 1:
raise Exception("Error compressing the project database")
log.info("Uploading project files ...")
exclude = ["sources/*", "project.db", "project.db.gz"]
if not config.results.create_zip:
exclude += ["results.zip"]
object_prefix = "results/projects/{}".format(project_id)
start_callback = lambda path: log.info(" {}".format(path))
if os.path.exists(compressed_db_path):
task.storage.upload(compressed_db_path, object_prefix=object_prefix, overwrite=True,
start_callback=start_callback)
task.storage.upload(projres.path, object_prefix=object_prefix, exclude=exclude, overwrite=True,
start_callback=start_callback)
if config.results.purge_after_upload:
log.info("Purging project files ...")
shutil.rmtree(projres.path)
finally:
if os.path.exists(temp_path):
shutil.rmtree(temp_path)
def execute(self):
workspace_path = os.path.join(self.results_path, self.args.workspace)
projects_path = os.path.join(workspace_path, "projects")
qc_path = os.path.join(workspace_path, "quality_control")
self.log.info("Preparing output path at {} ...".format(os.path.relpath(qc_path, self.runtime_path)))
self.create_output_path(qc_path)
loader = ChoiceLoader([
PackageLoader(__name__, 'qc_templates'),
FileSystemLoader(os.path.join(self.root_path, "web", "templates"))
])
env = Environment(loader=loader, autoescape=False)
env.globals.update(
version=VERSION,
creation_date=str(datetime.now())
)
env.filters["tojson"] = tojson
self.log.info("Looking for projects ...")
projects = []
quality_controls = []
for project_path in sorted(os.listdir(projects_path)):
project_path = os.path.join(projects_path, project_path)
proj_res = ProjectResults(path=project_path)
project = proj_res.load_def()
quality = proj_res.load_quality_control()
projects += [project]
quality_controls += [quality]
self.log.info("Processing {} projects ...".format(len(projects)))
for i, project in enumerate(projects):
self.log.info("Processing report for project {} ...".format(project["id"]))
quality = quality_controls[i]
t = env.get_template("project.html")
context=dict(
index=i,
project=project,
quality=quality,
projects=projects)
t.stream(context).dump(os.path.join(qc_path, "project_{}.html".format(project["id"])))
self.log.info("Generating index.html ...")
t = env.get_template("index.html")
t.stream(projects=projects).dump(os.path.join(qc_path, "index.html"))
def variants(project):
log = task.logger
log.info("--- [{0}] --------------------------------------------".format(project["id"]))
log.info("Calculating number of variants processed in each step ...")
proj_res = ProjectResults(project)
projdb = ProjectDb(project["db"])
counts = projdb.count_variants()
proj_res.save_quality_control("variants", counts)
projdb.close()
def __load_case_info(case, except_enabled=False, quality_enabled=False): wok = current_app.wok case_info = dict( id=case.id, name=case.name, state=case.state.title if case.state is not None else None,created=case.created, started=case.started, finished=case.finished, removed=case.removed) if case.state is not None: case_info["state"] = case.state.title if case.properties is not None: conf = get_project_conf() results_path = get_results_path(conf) project_path = os.path.join(results_path, case.properties["path"]) quality = None if quality_enabled: project_results = ProjectResults(path=project_path) quality = project_results.load_quality_control() zip_path = os.path.join(project_path, "results.zip") website_path = os.path.join(project_path, "website", "results", "project.tsv") case_info.update( analysis_type=case.properties["analysis_type"], results_available=os.path.exists(zip_path), website_available=os.path.exists(website_path), quality=quality if quality is not None and len(quality) > 0 else None) engine_case = wok.engine.case(case.engine_name) if engine_case is not None: exceptions = None if except_enabled: exceptions = __engine_case_exceptions(engine_case) case_info.update( state=engine_case.state.title, started=engine_case.started, finished=engine_case.finished, elapsed=engine_case.elapsed, progress=__engine_case_progress(engine_case), exceptions=exceptions) return case_info
def oncodrivefm(project):
log = task.logger
conf = task.conf
log.info("--- [{0}] --------------------------------------------".format(project["id"]))
config = GlobalConfig(conf)
paths = PathsConfig(config) # avoid that project conf override path configurations
config = GlobalConfig(conf, project["conf"])
ofm = OncodriveFm(config.oncodrivefm, paths, log)
log.info("Calculating quality indicators for OncodriveFM ...")
qc_data = quality_control(log, config, project, ofm)
project_results = ProjectResults(project)
project_results.save_quality_control("oncodrivefm", qc_data)
def pack_datasets(project):
log = task.logger
config = GlobalConfig(task.conf)
project_id = project["id"]
log.info("--- [{0}] --------------------------------------------".format(project_id))
if not config.results.create_zip:
log.info("Creation of the results compressed file is deactivated. Skipped.")
return
project_path = project["path"]
temp_path = project["temp_path"]
dest_path = os.path.join(project_path, "results.zip")
sigdb = SigDb(config.sigdb_path)
sigdb.open()
projdb = ProjectDb(project["db"])
projres = ProjectResults(project)
gene_sym = projdb.get_gene_symbols()
total_samples = projdb.get_total_affected_samples()
log.info("Compressing files ...")
arc = None
try:
arc = Archive(dest_path, mode="w", fmt="zip")
log.info(" Variant genes ...")
with ArcFile(task, arc, project_id, "variant_genes", "w") as vf:
write_line(vf, "PROJECT_ID", "CHR", "STRAND", "START", "ALLELE",
"GENE_ID", "SYMBOL", "VAR_IMPACT", "VAR_IMPACT_DESC",
"SAMPLE_FREQ", "SAMPLE_TOTAL", "SAMPLE_PROP",
"CODING_REGION", "PROTEIN_CHANGES", "INTOGEN_DRIVER", "XREFS")
for afg in projdb.affected_genes(join_variant=True, join_xrefs=True, join_rec=True):
var = afg.var
rec = afg.rec
start, end, ref, alt = var_to_tab(var)
xrefs = [xref for xref in var.xrefs]
if sigdb.exists_variant(var.chr, start):
xrefs += ["I:1"]
xrefs = ",".join(xrefs)
intogen_driver = 1 if sigdb.exists_gene(afg.gene_id) else 0
write_line(vf, project_id, var.chr, var.strand, start, "{0}/{1}".format(ref, alt),
afg.gene_id, gene_sym.get(afg.gene_id),
afg.impact, TransFIC.class_name(afg.impact),
rec.sample_freq or 0, total_samples, rec.sample_prop or 0,
afg.coding_region, afg.prot_changes, intogen_driver, xrefs)
log.info(" Variant samples ...")
with ArcFile(task, arc, project_id, "variant_samples", "w") as vf:
write_line(vf, "PROJECT_ID", "CHR", "STRAND", "START", "ALLELE", "SAMPLES")
for var in projdb.variants(join_samples=True):
start, end, ref, alt = var_to_tab(var)
write_line(vf, project_id, var.chr, var.strand, start, "{0}/{1}".format(ref, alt),
",".join([s.name for s in var.samples]))
log.info(" Consequences ...")
with ArcFile(task, arc, project_id, "consequences", "w") as cf:
write_line(cf, "PROJECT_ID", "CHR", "STRAND", "START", "ALLELE", "TRANSCRIPT_ID", "CT",
"GENE_ID", "SYMBOL", "UNIPROT_ID", "PROTEIN_ID", "PROTEIN_POS", "AA_CHANGE",
"SIFT_SCORE", "SIFT_TRANSFIC", "SIFT_TRANSFIC_CLASS",
"PPH2_SCORE", "PPH2_TRANSFIC", "PPH2_TRANSFIC_CLASS",
"MA_SCORE", "MA_TRANSFIC", "MA_TRANSFIC_CLASS",
"IMPACT", "IMPACT_CLASS")
for csq in projdb.consequences(join_variant=True):
var = csq.var
start, end, ref, alt = var_to_tab(var)
allele = "{0}/{1}".format(ref, alt)
uniprot = protein = protein_pos = aa_change = None
sift_score = sift_tfic = sift_tfic_class = None
pph2_score = pph2_tfic = pph2_tfic_class = None
ma_score = ma_tfic = ma_tfic_class = None
if so.match(csq.ctypes, so.ONCODRIVEFM):
uniprot, protein = csq.uniprot, csq.protein
if so.match(csq.ctypes, so.NON_SYNONYMOUS):
protein_pos, aa_change = csq.protein_pos, csq.aa_change
sift_score, sift_tfic, sift_tfic_class = csq.sift_score, csq.sift_tfic, TransFIC.class_name(csq.sift_tfic_class)
pph2_score, pph2_tfic, pph2_tfic_class = csq.pph2_score, csq.pph2_tfic, TransFIC.class_name(csq.pph2_tfic_class)
ma_score, ma_tfic, ma_tfic_class = csq.ma_score, csq.ma_tfic, TransFIC.class_name(csq.ma_tfic_class)
write_line(cf, project_id, var.chr, var.strand, start, allele, csq.transcript,
",".join(csq.ctypes), csq.gene, gene_sym.get(csq.gene),
uniprot, protein, protein_pos, aa_change,
sift_score, sift_tfic, sift_tfic_class,
pph2_score, pph2_tfic, pph2_tfic_class,
ma_score, ma_tfic, ma_tfic_class,
csq.impact, TransFIC.class_name(csq.impact))
log.info(" Genes ...")
with ArcFile(task, arc, project_id, "genes", "w") as gf:
write_line(gf, "PROJECT_ID", "GENE_ID", "SYMBOL", "FM_PVALUE", "FM_QVALUE", "FM_EXC_CAUSE",
"SAMPLE_FREQ", "SAMPLE_TOTAL", "SAMPLE_PROP",
"CLUST_ZSCORE", "CLUST_PVALUE", "CLUST_QVALUE", "CLUST_EXC_CAUSE", "CLUST_COORDS",
"INTOGEN_DRIVER", "XREFS")
for gene in projdb.genes(join_xrefs=True, join_rec=True):
if gene.rec.sample_freq is not None and gene.rec.sample_freq > 0:
intogen_driver = 1 if sigdb.exists_gene(gene.id) else 0
write_line(gf, project_id, gene.id, gene.symbol, gene.fm_pvalue, gene.fm_qvalue, gene.fm_exc_cause,
gene.rec.sample_freq, total_samples, gene.rec.sample_prop or 0,
gene.clust_zscore, gene.clust_pvalue, gene.clust_qvalue, gene.clust_exc_cause,
gene.clust_coords, intogen_driver, ",".join(gene.xrefs))
log.info(" Pathways ...")
with ArcFile(task, arc, project_id, "pathways", "w") as pf:
write_line(pf, "PROJECT_ID", "PATHWAY_ID", "GENE_COUNT", "FM_ZSCORE", "FM_PVALUE", "FM_QVALUE",
"SAMPLE_FREQ", "SAMPLE_TOTAL", "SAMPLE_PROP", "GENE_FREQ", "GENE_TOTAL", "GENE_PROP")
for pathway in projdb.pathways(join_rec=True):
if pathway.rec.sample_freq is not None and pathway.rec.sample_freq > 0:
write_line(pf, project_id, pathway.id, pathway.gene_count, pathway.fm_zscore, pathway.fm_pvalue, pathway.fm_qvalue,
pathway.rec.sample_freq or 0, total_samples, pathway.rec.sample_prop or 0,
pathway.rec.gene_freq or 0, pathway.gene_count, pathway.rec.gene_prop or 0)
if not config.skip_oncodrivefm:
log.info(" Genes per sample functional impact ...")
with ArcFile(task, arc, project_id, "fimpact.gitools.tdm", "w") as f:
write_line(f, "SAMPLE", "GENE_ID",
"SIFT_SCORE", "SIFT_TRANSFIC", "SIFT_TRANSFIC_CLASS",
"PPH2_SCORE", "PPH2_TRANSFIC", "PPH2_TRANSFIC_CLASS",
"MA_SCORE", "MA_TRANSFIC", "MA_TRANSFIC_CLASS")
for fields in projdb.sample_gene_fimpacts():
(gene, sample,
sift_score, sift_tfic, sift_tfic_class,
pph2_score, pph2_tfic, pph2_tfic_class,
ma_score, ma_tfic, ma_tfic_class) = fields
write_line(f, sample, gene,
sift_score, sift_tfic, TransFIC.class_name(sift_tfic_class),
pph2_score, pph2_tfic, TransFIC.class_name(pph2_tfic_class),
ma_score, ma_tfic, TransFIC.class_name(ma_tfic_class))
log.info("Saving project configuration ...")
with ArcFile(task, arc, project_id, "project", "w") as f:
names = ["PROJECT_ID", "ASSEMBLY", "SAMPLES_TOTAL"]
values = [project_id, project["assembly"], total_samples]
names, values = projres.get_annotations_to_save(config.project.annotations, project["annotations"], names=names, values=values)
tsv.write_line(f, *names)
tsv.write_line(f, *values, null_value="-")
finally:
if arc is not None:
arc.close()
projdb.close()
sigdb.close()
def oncodriveclust(project):
log = task.logger
conf = task.conf
log.info("--- [{0}] --------------------------------------------".format(project["id"]))
config = GlobalConfig(conf)
paths = PathsConfig(config) # avoid that project conf override path configurations
config = GlobalConfig(conf, project["conf"])
oclust = OncodriveClust(config.oncodriveclust, paths, log)
source_genes = {}
syn_genes = set()
selected_genes = set()
filter_genes = set()
threshold_genes = set()
source_samples = {}
selected_samples = set()
filter_samples = set()
threshold_samples = set()
selected_gene_sample_count = {} # number of samples for each selected gene
filter_gene_sample_count = {} # number of samples per each gene passing the filter
log.info("Retrieving gene alterations ...")
projdb = ProjectDb(project["db"])
data = set()
for csq in projdb.consequences(join_samples=True):
#filters={ProjectDb.CSQ_CTYPES : so.PROTEIN_AFFECTING | so.SYNONYMOUS}):
is_selected = so.match(csq.ctypes, so.ONCODRIVECLUST)
is_synonymous = so.match(csq.ctypes, so.SYNONYMOUS)
if csq.gene not in source_genes:
source_genes[csq.gene] = gene_index = len(source_genes)
if is_selected:
selected_genes.add(gene_index)
if is_synonymous:
syn_genes.add(gene_index)
for sample in csq.var.samples:
if sample.name not in source_samples:
source_samples[sample.name] = sample_index = len(source_samples)
if is_selected:
selected_samples.add(sample_index)
data.add((csq.gene, sample_index))
projdb.close()
log.info("Counting selected, filtered and threshold ...")
# calculate selected and filter counts
data2 = set()
for gene, sample_index in data:
gene_index = source_genes[gene]
if gene_index not in selected_gene_sample_count:
selected_gene_sample_count[gene_index] = 1
else:
selected_gene_sample_count[gene_index] += 1
if oclust.filter.valid(gene):
data2.add((gene_index, sample_index))
filter_genes.add(gene_index)
filter_samples.add(sample_index)
if gene_index not in filter_gene_sample_count:
filter_gene_sample_count[gene_index] = 1
else:
filter_gene_sample_count[gene_index] += 1
# calculate threshold counts
for gene_index, sample_index in data2:
if selected_gene_sample_count[gene_index] >= oclust.samples_threshold:
threshold_genes.add(gene_index)
threshold_samples.add(sample_index)
log.info("Counting significant genes ...")
# significance of q-values
projdb = ProjectDb(project["db"])
sig_thresholds = [0.0, 0.001, 0.005] + [i / 100.0 for i in range(1, 11)] + [1.0]
sig_count = [0] * len(sig_thresholds)
for gene in projdb.genes():
if gene.id in source_genes and source_genes[gene.id] in threshold_genes:
i = 0
while i < len(sig_thresholds) and gene.fm_qvalue > sig_thresholds[i]:
i += 1
for j in range(i, len(sig_count)):
sig_count[j] += 1
projdb.close()
source_genes_count = len(source_genes)
syn_genes_count = len(syn_genes)
selected_genes_count = len(selected_genes)
filter_genes_count = len(filter_genes)
threshold_genes_count = len(threshold_genes)
source_samples_count = len(source_samples)
selected_samples_count = len(selected_samples)
filter_samples_count = len(filter_samples)
threshold_samples_count = len(threshold_samples)
sorted_filter_genes = sorted(filter_genes, reverse=True, key=lambda gi: filter_gene_sample_count[gi])
qc_data = dict(
source=dict(
genes=sorted(source_genes.keys(), key=lambda k: source_genes[k]),
genes_count=source_genes_count,
genes_lost_count=max(0, source_genes_count - syn_genes_count - threshold_genes_count),
samples=sorted(source_samples.keys(), key=lambda k: source_samples[k]),
samples_count=source_samples_count),
samples_lost_count=max(0, source_samples_count - threshold_samples_count),
synonymous=dict(
genes=sorted(syn_genes),
genes_count=syn_genes_count,
ratio=(float(syn_genes_count) / selected_genes_count) if selected_genes_count > 0 else 0),
selected=dict(
genes=sorted(selected_genes),
genes_count=selected_genes_count,
genes_lost=sorted(set(source_genes.values()) - syn_genes - selected_genes),
genes_lost_count=max(0, source_genes_count - syn_genes_count - selected_genes_count),
samples=sorted(selected_samples),
samples_count=selected_samples_count,
samples_lost=sorted(set(source_samples.values()) - selected_samples),
samples_lost_count=max(0, source_samples_count - selected_samples_count)),
filter=dict(
genes=sorted_filter_genes,
genes_count=filter_genes_count,
genes_lost=sorted(selected_genes - filter_genes),
genes_lost_count=max(0, selected_genes_count - filter_genes_count),
genes_sample_count=[filter_gene_sample_count[gene_index] for gene_index in sorted_filter_genes],
samples=sorted(filter_samples),
samples_count=filter_samples_count,
samples_lost=sorted(selected_samples - filter_samples),
samples_lost_count=max(0, selected_samples_count - filter_samples_count)),
threshold=dict(
genes=sorted(threshold_genes),
genes_count=threshold_genes_count,
genes_lost=sorted(filter_genes - threshold_genes),
genes_lost_count=max(0, filter_genes_count - threshold_genes_count),
samples=sorted(threshold_samples),
samples_count=threshold_samples_count,
samples_threshold=oclust.samples_threshold,
samples_lost=sorted(filter_samples - threshold_samples),
samples_lost_count=max(0, filter_samples_count - threshold_samples_count)),
results=dict(
sig_thresholds=sig_thresholds[1:],
sig_count=sig_count[1:])
)
project_results = ProjectResults(project)
project_results.save_quality_control("oncodriveclust", qc_data)
def init_project(logger, config, paths, storage, project):
project_id = project["id"]
results_path = paths.results_path()
project_path = paths.project_path(project_id)
project_temp_path = paths.project_temp_path(project_path)
if config.results.purge_on_start:
logger.info(" Purging previous results ...")
if os.path.isdir(project_path):
logger.info(" {} ...".format(os.path.relpath(project_path, results_path)))
shutil.rmtree(project_path)
#if os.path.isdir(project_temp_path):
# logger.info(" {} ...".format(os.path.relpath(project_temp_path, results_path)))
# shutil.rmtree(project_temp_path)
for obj_name in storage.list_objects(prefix="results/"):
logger.info(" {} ...".format(obj_name))
storage.delete_object("results/{}".format(obj_name))
ensure_path_exists(project_path)
ensure_path_exists(project_temp_path)
projdb_path = os.path.join(project_path, "project.db")
if "annotations" in project:
annotations = project["annotations"]
if not Data.is_element(annotations):
logger.warn("Overriding project annotations field with an empty dictionary")
project["annotations"] = annotations = Data.element()
else:
project["annotations"] = annotations = Data.element()
# for backward compatibility
for key in project.keys():
if key not in ["id", "assembly", "files", "storage_objects", "annotations", "conf", "oncodriveclust", "oncodrivefm"]:
value = project[key]
del project[key]
annotations[key] = value
project["conf"] = pconf = project.get("conf") or Data.element()
if not Data.is_element(pconf):
logger.warn("Overriding project conf field with an empty dictionary")
project["conf"] = pconf = Data.element()
# for backward compatibility
for key in project.keys():
if key in ["oncodriveclust", "oncodrivefm"]:
value = project[key]
del project[key]
pconf[key] = value
project["path"] = project_path
project["temp_path"] = project_temp_path
project["db"] = projdb_path
if "assembly" not in project:
project["assembly"] = DEFAULT_ASSEMBLY
missing_objects = []
for obj_name in project["storage_objects"]:
if not storage.exists_object(obj_name):
missing_objects += [obj_name]
if len(missing_objects) > 0:
raise InternalError("Project {0} references some missing objects:\n{1}".format(project_id, "\n".join(missing_objects)))
project["files"] = [str(f) for f in project["files"]] #unicode is not json serializable
project["storage_objects"] = [str(f) for f in project["storage_objects"]] #unicode is not json serializable
project = project.to_native()
# save project.conf
projres = ProjectResults(project)
projres.save_def()
return project
def create_datasets(project):
log = task.logger
config = GlobalConfig(task.conf)
paths = PathsConfig(config)
project_id = project["id"]
log.info("--- [{0}] --------------------------------------------".format(project_id))
project_path = project["path"]
temp_path = project["temp_path"]
datasets_path = paths.project_results_path(project_path)
ensure_path_exists(datasets_path)
sigdb = SigDb(config.sigdb_path)
sigdb.open()
projdb = ProjectDb(project["db"])
gene_sym = projdb.get_gene_symbols()
total_samples = projdb.get_total_affected_samples()
log.info("Exporting variant genes ...")
vf = open_dataset(project_id, project_path, datasets_path, "variant_gene", "w", log)
tsv.write_param(vf, "SAMPLE_TOTAL", total_samples)
tsv.write_line(vf, "VAR_ID", "CHR", "STRAND", "START", "ALLELE",
"GENE_ID", "IMPACT", "IMPACT_CLASS",
"SAMPLE_FREQ", "SAMPLE_PROP",
"CODING_REGION", "PROTEIN_CHANGES", "INTOGEN_DRIVER", "XREFS")
sf = open_dataset(project_id, project_path, datasets_path, "variant-samples", "w", log)
tsv.write_line(sf, "VAR_ID", "CHR", "STRAND", "START", "ALLELE", "SAMPLE")
count = 0
for afg in projdb.affected_genes(join_variant=True, join_samples=True, join_xrefs=True, join_rec=True):
var = afg.var
rec = afg.rec
start, end, ref, alt = var_to_tab(var)
allele = "{0}/{1}".format(ref, alt)
xrefs = [xref for xref in var.xrefs]
if sigdb.exists_variant(var.chr, start):
xrefs += ["I:1"]
xrefs = ",".join(xrefs)
intogen_driver = 1 if sigdb.exists_gene(afg.gene_id) else 0
tsv.write_line(vf, var.id, var.chr, var.strand, start, allele,
afg.gene_id, afg.impact, TransFIC.class_name(afg.impact),
rec.sample_freq, rec.sample_prop,
afg.coding_region, afg.prot_changes, intogen_driver, xrefs, null_value="\N")
for sample in var.samples:
tsv.write_line(sf, var.id, var.chr, var.strand, start, allele, sample.name, null_value="\N")
count += 1
vf.close()
sf.close()
log.info(" {0} variant genes".format(count))
log.info("Exporting consequences ...")
cf = open_dataset(project_id, project_path, datasets_path, "consequence", "w", log)
tsv.write_line(cf, "VAR_ID", "CHR", "STRAND", "START", "ALLELE", "TRANSCRIPT_ID",
"CT", "GENE_ID", "SYMBOL", "UNIPROT_ID", "PROTEIN_ID", "PROTEIN_POS", "AA_CHANGE",
"SIFT_SCORE", "SIFT_TRANSFIC", "SIFT_TRANSFIC_CLASS",
"PPH2_SCORE", "PPH2_TRANSFIC", "PPH2_TRANSFIC_CLASS",
"MA_SCORE", "MA_TRANSFIC", "MA_TRANSFIC_CLASS",
"IMPACT", "IMPACT_CLASS")
count = 0
for csq in projdb.consequences(join_variant=True):
var = csq.var
start, end, ref, alt = var_to_tab(var)
allele = "{0}/{1}".format(ref, alt)
uniprot = protein = protein_pos = aa_change = None
sift_score = sift_tfic = sift_tfic_class = None
pph2_score = pph2_tfic = pph2_tfic_class = None
ma_score = ma_tfic = ma_tfic_class = None
if so.match(csq.ctypes, so.ONCODRIVEFM):
uniprot, protein = csq.uniprot, csq.protein
if so.match(csq.ctypes, so.NON_SYNONYMOUS):
protein_pos, aa_change = csq.protein_pos, csq.aa_change
sift_score, sift_tfic, sift_tfic_class = csq.sift_score, csq.sift_tfic, TransFIC.class_name(csq.sift_tfic_class)
pph2_score, pph2_tfic, pph2_tfic_class = csq.pph2_score, csq.pph2_tfic, TransFIC.class_name(csq.pph2_tfic_class)
ma_score, ma_tfic, ma_tfic_class = csq.ma_score, csq.ma_tfic, TransFIC.class_name(csq.ma_tfic_class)
tsv.write_line(cf, var.id, var.chr, var.strand, start, allele, csq.transcript,
",".join(csq.ctypes), csq.gene, gene_sym.get(csq.gene),
uniprot, protein, protein_pos, aa_change,
sift_score, sift_tfic, sift_tfic_class,
pph2_score, pph2_tfic, pph2_tfic_class,
ma_score, ma_tfic, ma_tfic_class,
csq.impact, TransFIC.class_name(csq.impact), null_value="\N")
count += 1
cf.close()
log.info(" {0} consequences".format(count))
log.info("Exporting genes ...")
gf = open_dataset(project_id, project_path, datasets_path, "gene", "w", log)
tsv.write_param(gf, "SAMPLE_TOTAL", total_samples)
tsv.write_line(gf, "GENE_ID", "FM_PVALUE", "FM_QVALUE", "FM_EXC_CAUSE",
"CLUST_ZSCORE", "CLUST_PVALUE", "CLUST_QVALUE", "CLUST_EXC_CAUSE", "CLUST_COORDS",
"SAMPLE_FREQ", "SAMPLE_PROP", "INTOGEN_DRIVER")
for gene in projdb.genes(join_rec=True):
rec = gene.rec
if rec.sample_freq is None or rec.sample_freq == 0:
continue
intogen_driver = 1 if sigdb.exists_gene(gene.id) else 0
tsv.write_line(gf, gene.id, gene.fm_pvalue, gene.fm_qvalue, gene.fm_exc_cause,
gene.clust_zscore, gene.clust_pvalue, gene.clust_qvalue, gene.clust_exc_cause, gene.clust_coords,
rec.sample_freq or 0, rec.sample_prop or 0,
intogen_driver, null_value="\N")
gf.close()
log.info("Exporting pathways ...")
pf = open_dataset(project_id, project_path, datasets_path, "pathway", "w", log)
tsv.write_param(pf, "SAMPLE_TOTAL", total_samples)
tsv.write_line(pf, "PATHWAY_ID", "GENE_COUNT", "FM_ZSCORE", "FM_PVALUE", "FM_QVALUE",
"SAMPLE_FREQ", "SAMPLE_PROP", "GENE_FREQ", "GENE_TOTAL", "GENE_PROP")
for pathway in projdb.pathways(join_rec=True):
rec = pathway.rec
if rec.sample_freq is None or rec.sample_freq == 0:
continue
tsv.write_line(pf, pathway.id, pathway.gene_count, pathway.fm_zscore, pathway.fm_pvalue, pathway.fm_qvalue,
rec.sample_freq or 0, rec.sample_prop or 0, rec.gene_freq or 0, pathway.gene_count, rec.gene_prop or 0, null_value="\N")
pf.close()
if not config.skip_oncodrivefm:
log.info("Exporting genes per sample functional impact ...")
with open_dataset(project_id, project_path, datasets_path, "gene_sample-fimpact", "w", log) as f:
tsv.write_line(f, "GENE_ID", "SAMPLE",
"SIFT_SCORE", "SIFT_TRANSFIC", "SIFT_TRANSFIC_CLASS",
"PPH2_SCORE", "PPH2_TRANSFIC", "PPH2_TRANSFIC_CLASS",
"MA_SCORE", "MA_TRANSFIC", "MA_TRANSFIC_CLASS")
for fields in projdb.sample_gene_fimpacts():
(gene, sample,
sift_score, sift_tfic, sift_tfic_class,
pph2_score, pph2_tfic, pph2_tfic_class,
ma_score, ma_tfic, ma_tfic_class) = fields
tsv.write_line(f, gene, sample,
sift_score, sift_tfic, TransFIC.class_name(sift_tfic_class),
pph2_score, pph2_tfic, TransFIC.class_name(pph2_tfic_class),
ma_score, ma_tfic, TransFIC.class_name(ma_tfic_class), null_value="\N")
projdb.close()
sigdb.close()
log.info("Saving project configuration ...")
projres = ProjectResults(project)
with open_dataset(project_id, project_path, datasets_path, "project.tsv", "w", log) as f:
names = ["ASSEMBLY", "SAMPLES_TOTAL"]
values = [project["assembly"], total_samples]
names, values = projres.get_annotations_to_save(config.project.annotations, project["annotations"], names=names, values=values)
tsv.write_line(f, *names)
tsv.write_line(f, *values, null_value="\N")
projects_port = task.ports("projects_out")
projects_port.send(project)