def __init__(self, *arg):
"""
Return Rect that is subclassed from java.awt.Rectangle.
Alternative arguments:
* x, y, width, height
* (x, y), (width, height)
* (x, y, width, height)
* Rect
* Obj with rect attribute
Rect has the attributes::
x, y, width, height
top, left, bottom, right
topleft, bottomleft, topright, bottomright
midtop, midleft, midbottom, midright
center, centerx, centery
size, w, h
Module initialization places pyj2d.Rect in module's namespace.
"""
def unpack(arg, lst=[]):
for x in arg:
if not isinstance(x, tuple):
lst.append(x)
else:
lst = unpack(x, lst)
return lst
try:
x = arg[0]
y = arg[1]
w = arg[2]
h = arg[3]
except IndexError:
try:
x = arg[0][0]
y = arg[0][1]
w = arg[0][2]
h = arg[0][3]
except (IndexError, TypeError, AttributeError):
arg = unpack(arg)
try:
x = arg[0]
y = arg[1]
w = arg[2]
h = arg[3]
except IndexError:
if hasattr(arg[0], 'rect'):
arg[0] = arg[0].rect
x = arg[0].x
y = arg[0].y
w = arg[0].width
h = arg[0].height
try:
Rectangle.__init__(self, x, y, w, h)
except TypeError:
Rectangle.__init__(self, int(x), int(y), int(w), int(h))
def __init__(self, *arg):
"""
Return Rect that is subclassed from java.awt.Rectangle.
Alternative arguments:
* x,y,w,h
* (x,y),(w,h)
* (x,y,w,h)
* Rect
* Obj with rect attribute
Rect has the attributes::
x, y, width, height
Additional Rect attributes::
top, left, bottom, right, topleft, bottomleft, topright, bottomright,
midtop, midleft, midbottom, midright, center, centerx, centery,
size, w, h.
Module initialization places pyj2d.Rect in module's namespace.
"""
def unpack(arg, lst=[]):
for x in arg:
if not isinstance(x, tuple):
lst.append(x)
else:
lst = unpack(x, lst)
return lst
try:
x,y,w,h = arg[0], arg[1], arg[2], arg[3]
except IndexError:
try:
x,y,w,h = arg[0][0], arg[0][1], arg[0][2], arg[0][3]
except (IndexError, TypeError, AttributeError):
arg = unpack(arg)
try:
x,y,w,h = arg[0], arg[1], arg[2], arg[3]
except IndexError:
if hasattr(arg[0], 'rect'):
arg[0] = arg[0].rect
x,y,w,h = arg[0].x, arg[0].y, arg[0].width, arg[0].height
try:
Rectangle.__init__(self, x, y, w, h)
except TypeError:
Rectangle.__init__(self, int(x), int(y), int(w), int(h))
def showImgWithFullWindow(width=None, height=None):
""" This function shows the image from current IDV window while in GUI mode. optional arguments are width and height in pixels, they
currently default to 600 and 400"""
from java.util import Base64 ##only in java8
from javax.imageio import ImageIO
from java.io import ByteArrayOutputStream
from ucar.unidata.ui.ImageUtils import resize, toBufferedImage
import java
import java.awt.Robot as Robot
import java.awt.Rectangle as Rectangle
import java.awt.Toolkit as Toolkit
from ucar.unidata.util import Misc
VM = idv.getViewManager()
myframe = VM.getDisplayWindow().getComponent()
robotx = Robot(myframe.getGraphicsConfiguration().getDevice())
VM.toFront()
#robotx.delay(250)
Misc.sleep(350)
pause()
img = robotx.createScreenCapture(
Rectangle(myframe.getX(), myframe.getY(), myframe.getWidth(),
myframe.getHeight()))
if width != None and height != None:
img = toBufferedImage(resize(img, width, height))
bos = ByteArrayOutputStream()
ImageIO.write(img, "png",
Base64.getEncoder().wrap(bos))
data = bos.toString("UTF-8")
return {"display": "image", "data": data}
def showImgWithLegend(width=None, height=None):
""" This function shows the image and legend from current IDV window while in GUI mode. Optional arguments are width and height in pixels, they currently default to 600 and 400"""
from java.util import Base64 ##only in java8
from javax.imageio import ImageIO
from java.io import ByteArrayOutputStream
from ucar.unidata.ui.ImageUtils import resize, toBufferedImage
import java
import java.awt.Robot as Robot
import java.awt.Rectangle as Rectangle
import java.awt.Toolkit as Toolkit
from ucar.unidata.util import Misc
VM = idv.getViewManager()
VMC = VM.getContents()
VMCC = VMC.getComponent(
1
) # the view and legend ; 0 is left most part of view window with controls for perspective views
siz = VMCC.getSize()
loc = VMCC.getLocationOnScreen()
gc = VMCC.getGraphicsConfiguration()
loc.x -= gc.getBounds().x
loc.y -= gc.getBounds().y
robotx = Robot(gc.getDevice())
VM.toFront()
Misc.sleep(250)
img = robotx.createScreenCapture(
Rectangle(loc.x, loc.y, siz.width, siz.height))
if width != None and height != None:
img = toBufferedImage(resize(img, width, height))
bos = ByteArrayOutputStream()
ImageIO.write(img, "png",
Base64.getEncoder().wrap(bos))
data = bos.toString("UTF-8")
return {"display": "image", "data": data}
def union_ip(self, rect):
"""
Change this rect to represent the union of rect and this rect.
"""
r = Rectangle.union(self, rect)
self.setLocation(r.x, r.y)
self.setSize(r.width, r.height)
return None
def unionall(self, rect_list):
"""
Return Rect representing the union of rect list and this rect.
"""
r = self
for rect in rect_list:
r = Rectangle.union(r, rect)
return Rect(r.x, r.y, r.width, r.height)
def run(self):
try:
filepath = self.makeFilePath()
if os.path.exists(filepath):
return
x, y, k = self.coords
width, height, n_layers = self.dimensions
# Cube's field of view in XY
fov = Rectangle(x, y, width, height)
# Join the bounds of the layers
r = None
for b in self.bounds[k:min(k + n_layers, len(bounds))]:
if r is None:
r = Rectangle(b.x, b.y, b.width, b.height)
else:
r.add(b)
if not fov.intersects(r):
# Would be empty
return
drawImage = Graphics2D.getDeclaredMethod(
"drawImage", [Image, AffineTransform, ImageObserver])
drawImage.setAccessible(True)
dispose = Graphics.getDeclaredMethod("dispose", [])
dispose.setAccessible(True)
# Populate and write cube
stack = ImageStack(width, height)
for layer in self.layers[k:min(k + n_layers, len(self.layers))]:
img = layer.getProject().getLoader().getFlatAWTImage(
layer, fov, 1.0, -1, ImagePlus.GRAY8, Patch, None, False,
Color.black)
bi = BufferedImage(img.getWidth(None), img.getHeight(None),
BufferedImage.TYPE_BYTE_GRAY)
g = bi.createGraphics()
aff = AffineTransform(1, 0, 0, 1, 0, 0)
#g.drawImage(img, aff, None) # Necessary to bypass issues that result in only using 7-bits and with the ByteProcessor constructor
drawImage.invoke(g, [img, aff, None])
#g.dispose()
dispose.invoke(g, [])
g = None
img = None
stack.addSlice("", ByteProcessor(bi))
bi.flush()
bi = None
imp = ImagePlus("x=%s y=%s k=%s" % (x, y, k), stack)
Utils.ensure(filepath)
FileSaver(imp).saveAsZip(filepath)
imp.flush()
imp = None
except:
e = sys.exc_info()
System.out.println("Error:" + str(e[0]) + "\n" + str(e[1]) + "\n" +
str(e[2]))
System.out.println(traceback.format_exception(e[0], e[1], e[2]))
def run(title):
gd = ij.gui.GenericDialog('Record Desktop')
gd.addNumericField('Max. frames:', 50, 0)
gd.addNumericField('Milisecond interval:', 300, 0)
gd.addSlider('Start in (seconds):', 0, 20, 5)
gd.showDialog()
if gd.wasCanceled():
return
n_frames = int(gd.getNextNumber())
interval = gd.getNextNumber() / 1000.0 # in seconds
delay = int(gd.getNextNumber())
snaps = []
try:
while delay > 0:
ij.IJ.showStatus('Starting in ' + str(delay) + 's.')
time.sleep(1) # one second
delay -= 1
ij.IJ.showStatus('')
ij.IJ.showStatus("Starting...")
out.println("Starting...")
# start capturing
robot = Robot()
box = Rectangle(ij.IJ.getScreenSize())
start = currentTimeMillis() / 1000.0 # in seconds
last = start
intervals = []
real_interval = 0
# Initial shot
snaps.append(robot.createScreenCapture(box))
while len(snaps) < n_frames and last - start < n_frames * interval:
now = currentTimeMillis() / 1000.0 # in seconds
real_interval = now - last
if real_interval >= interval:
last = now
snaps.append(robot.createScreenCapture(box))
intervals.append(real_interval)
else:
time.sleep(interval / 5) # time in seconds
# Create stack
out.println("End")
awt = snaps[0]
stack = ij.ImageStack(awt.getWidth(None), awt.getHeight(None), None)
t = 0
for snap, real_interval in zip(snaps, intervals):
stack.addSlice(str(ij.IJ.d2s(t, 3)),
ij.ImagePlus('', snap).getProcessor())
snap.flush()
t += real_interval
ImagePlus("Desktop recording", stack).show()
except Exception, e:
print "Some error ocurred:"
print e
for snap in snaps:
snap.flush()
def getViewFromImp(imp, r=None):
# r is a java.awt.rectangle
im = IL.wrapByte(imp)
if r is None:
r = Rectangle(0, 0, imp.getWidth(), imp.getHeight())
v = Views.zeroMin(
Views.interval(im, [r.x, r.y],
[r.x + r.width - 1, r.y + r.height - 1]))
return v
def unionall_ip(self, rect_list):
"""
Change this rect to represent the union of rect list and this rect.
"""
r = self
for rect in rect_list:
r = Rectangle.union(r, rect)
self.setLocation(r.x, r.y)
self.setSize(r.width, r.height)
return None
def generate_image(width, height):
renderer = StaticRenderer()
renderer.setTree(GVTBuilder().build(context, document))
transform = AffineTransform()
transform.translate(-svg_x, -svg_y)
transform.scale(width / svg_width, height / svg_height)
renderer.setTransform(transform)
renderer.updateOffScreen(width, height)
renderer.repaint(Rectangle(0, 0, width, height))
return renderer.getOffScreen()
def __init__(self, *arg):
"""
Return Rect that is subclassed from java.awt.Rectangle.
Alternative arguments:
* x,y,w,h
* (x,y),(w,h)
* (x,y,w,h)
* Rect
* Obj with rect attribute
Rect has the attributes::
x, y, width, height
Additional Rect attributes::
top, left, bottom, right, topleft, bottomleft, topright, bottomright,
midtop, midleft, midbottom, midright, center, centerx, centery,
size, w, h.
Module initialization places pyj2d.Rect in module's namespace.
"""
try:
x, y, w, h = arg[0], arg[1], arg[2], arg[3]
except IndexError:
try:
x, y, w, h = arg[0].rect.x, arg[0].rect.y, arg[
0].rect.width, arg[0].rect.height
except AttributeError:
try:
x, y, w, h = arg[0].x, arg[0].y, arg[0].width, arg[
0].height
except AttributeError:
try:
x, y, w, h = arg[0][0], arg[0][1], arg[1][0], arg[1][1]
except IndexError:
x, y, w, h = arg[0][0], arg[0][1], arg[0][2], arg[0][3]
try:
Rectangle.__init__(self, x, y, w, h)
except TypeError:
Rectangle.__init__(self, int(x), int(y), int(w), int(h))
def __init__(self, *arg):
"""
Return Rect that is subclassed from java.awt.Rectangle.
Alternative arguments:
* x,y,w,h
* (x,y),(w,h)
* (x,y,w,h)
* Rect
* Obj with rect attribute
Rect has the attributes::
x, y, width, height
Additional Rect attributes::
top, left, bottom, right, topleft, bottomleft, topright, bottomright,
midtop, midleft, midbottom, midright, center, centerx, centery,
size, w, h.
Module initialization places pyj2d.Rect in module's namespace.
"""
try:
x,y,w,h = arg[0], arg[1], arg[2], arg[3]
except IndexError:
try:
x,y,w,h = arg[0].rect.x, arg[0].rect.y, arg[0].rect.width, arg[0].rect.height
except AttributeError:
try:
x,y,w,h = arg[0].x, arg[0].y, arg[0].width, arg[0].height
except AttributeError:
try:
x,y,w,h = arg[0][0], arg[0][1], arg[1][0], arg[1][1]
except IndexError:
x,y,w,h = arg[0][0], arg[0][1], arg[0][2], arg[0][3]
try:
Rectangle.__init__(self, x, y, w, h)
except TypeError:
Rectangle.__init__(self, int(x), int(y), int(w), int(h))
def roiModified(self, imp, ID):
""" When the ROI of the active image changes, update the textfield values. """
if imp != IJ.getImage():
return # ignore if it's not the active image
roi = imp.getRoi()
if not roi or Roi.RECTANGLE != roi.getType():
return # none, or not a rectangle ROI
bounds = roi.getBounds()
if 0 == roi.getBounds().width + roi.getBounds().height:
bounds = Rectangle(0, 0, imp.getWidth(), imp.getHeight())
self.textfields[0].setText(str(bounds.x))
self.textfields[1].setText(str(bounds.y))
self.textfields[2].setText(str(bounds.width))
self.textfields[3].setText(str(bounds.height))
def fixAreatreeArea(areatree):
# Append a 1-pixel square area for nodes without any area
# this will fix using findZDisplayables for finding areatree,
# including connector.getTargets(), connector.getOrigins() etc.
if not isinstance(areatree, AreaTree):
print "Error: input must be an AreaTree"
return
root = areatree.getRoot()
if root is None:
return
for nd in root.getSubtreeNodes():
a = nd.getArea()
a.add(Area(Rectangle(int(nd.getX()), int(nd.getY()), 1, 1)))
nd.setData(a)
def checkColor(self, SCREEN):
print" checkColor !!"
i = Robot().createScreenCapture(Rectangle(SCREEN.getX(),
SCREEN.getY(), self.patternW, self.patternH))
bColor = Color(200, 4, 4)
pColor = Color(45, 48, 177)
print"self.patternW:"+self.patternW+ " self.patternH:"+self.patternH
for x in range(0, self.patternW):
for y in range(0, self.patternH):
print" color"
print i.getRGB(x, y)
if bColor == Color(i.getRGB(x, y)):
return "B"
if pColor == Color(i.getRGB(x, y)):
return "P"
def transformRefView(self, ref):
""" Transforms ref src_rect from physical units to pixels
Parameters
----------
ref : list
Rectangle (x, y, width, height) to display. x and y are coordinates of
upper-left corner of the Rectangle. ref is in physical units.
"""
x = int(self.calibration.getRawX(ref[0]))
y = int(self.calibration.getRawY(ref[1]))
width = int(self.calibration.getRawX(ref[2]))
height = int(self.calibration.getRawY(ref[3]))
return Rectangle(x, y, width, height)
def save_screenshot_to(self, path):
"""Saves a screenshot to the specified file.
The directory holding the file must exist or an exception is raised.
"""
path = os.path.abspath(path.replace('/', os.sep))
if not os.path.exists(os.path.dirname(path)):
raise RuntimeError(
"Directory '%s' where to save the screenshot does "
"not exist" % os.path.dirname(path))
screensize = Toolkit.getDefaultToolkit().getScreenSize()
rectangle = Rectangle(0, 0, screensize.width, screensize.height)
image = Robot().createScreenCapture(rectangle)
ImageIO.write(image, "jpg", File(path))
print "Screenshot saved to '%s'" % path
return path
def isEmpty(self):
# Check if the cube would have any data
x, y, k = self.coords
width, height, n_layers = self.dimensions
# Cube's field of view in XY
fov = Rectangle(x, y, width, height)
# Join the bounds of the layers
r = None
for b in self.bounds[k:min(k + n_layers, len(bounds))]:
if r is None and b is not None: # can be none when the Layer is empty
r = Rectangle(b.x, b.y, b.width, b.height)
elif b is not None:
r.add(b)
# Return True if not intersecting
return r is None or not fov.intersects(r)
def exportFlatRoi(project, scaleFactor, x, y, w, h, layer, saveName):
loader = project.getLoader()
rectangle = Rectangle(x-int(w/2),y-int(h/2),w,h)
patches = layer.find(Patch, x, y)
print patches
# IJ.log('patches' + str(patches))
for p, patch in enumerate(patches):
visible = patch.visible
patch.visible = True
tiles = ArrayList()
tiles.add(patch)
print 'tiles',tiles
print 'rectangle',rectangle
IJ.log('Patch ' + str(patch) + ' cropped with rectangle ' + str(rectangle) )
imp = loader.getFlatImage(layer, rectangle, scaleFactor, 0x7fffffff, ImagePlus.GRAY8, Patch, tiles, True, Color.black, None)
exportName = saveName + '_' + str(int(p)) + '.tif'
IJ.save(imp, exportName)
patch.visible = visible
def rigidAlignment(projectPath, params, name = '', boxFactor = 1):
# rigid alignment outside trakem2 with register virtual stack plugin because bug in trakem2
projectFolder = os.path.dirname(projectPath)
projectName = os.path.splitext(os.path.basename(projectPath))[0]
project, loader, layerset, nLayers = openTrakemProject(projectPath)
exportForRigidAlignmentFolder = mkdir_p(os.path.join(projectFolder, 'exportForRigidAlignment'))
resultRigidAlignmentFolder = mkdir_p(os.path.join(projectFolder, 'resultRigidAlignment'))
bb = layerset.get2DBounds()
roi = Rectangle(int(bb.width/2 * (1 - boxFactor)), int(bb.height/2 * (1 - boxFactor)), int(bb.width*boxFactor), int(bb.height*boxFactor))
boxPath = os.path.join(resultRigidAlignmentFolder, 'alignmentBox.txt')
writeRectangle(roi, boxPath)
exportFlat(project, exportForRigidAlignmentFolder, 1, baseName = 'exportForRigidAlignment', roi = roi)
referenceName = naturalSort(os.listdir(exportForRigidAlignmentFolder))[0]
use_shrinking_constraint = 0
IJ.log('Rigid alignment with register virtual stack')
Register_Virtual_Stack_MT.exec(exportForRigidAlignmentFolder, resultRigidAlignmentFolder, resultRigidAlignmentFolder, referenceName, params, use_shrinking_constraint)
time.sleep(2)
IJ.log('Warning: rigidAlignment closing all existing windows')
WindowManager.closeAllWindows() # problematic because it also closes the log window
# IJ.getImage().close()
for l, layer in enumerate(layerset.getLayers()):
imagePath = os.path.join(exportForRigidAlignmentFolder, 'exportForRigidAlignment_' + str(l).zfill(4) + '.tif')
transformPath = os.path.join(resultRigidAlignmentFolder, 'exportForRigidAlignment_' + str(l).zfill(4) + '.xml')
aff = getAffFromRVSTransformPath(transformPath)
for patch in layer.getDisplayables(Patch):
patch.setLocation(patch.getX()-roi.x, patch.getY()-roi.y) # compensate for the extracted bounding box
# patch.setLocation(roi.x, roi.y) # compensate for the extracted bounding box
currentAff = patch.getAffineTransform()
currentAff.preConcatenate(aff)
patch.setAffineTransform(currentAff)
resizeDisplay(layerset)
project.save()
closeProject(project)
IJ.log('All LM layers have been aligned in: ' + projectPath)
def __init__(self):
dataDir = Settings.dataDir + 'WorkingWithBarcodeRecognition/AdvancedBarcodeRecognitionFeatures/ReadBarcodeFromSpecificRegion/'
# Open the stream. Read only access is enough for Aspose.BarCode to load an image.
stream = FileInputStream(dataDir + "test.png")
# Create an instance of BarCodeReader class
# and specify an area to look for the barcode
barcode_reader_type = BarCodeReadType
reader = BarCodeReader(stream, Rectangle(0, 0, 10, 50),
barcode_reader_type.Code39Standard)
# TRead all barcodes in the provided area
while (reader.read()):
# Display the codetext and symbology type of the barcode found
print "Codetext: "
print reader.getCodeText()
print " Symbology: "
print reader.getReadType()
# Close reader
reader.close()
affineCroppedFolder = os.path.join(LMFolder,
'affineCropped_' + channel)
LMMosaicsPath = fc.cleanLinuxPath(
os.path.join(
LMFolder, 'exported_downscaled_1_' + channel,
'exported_downscaled_1_' + channel + '_' +
str(l).zfill(4) + '.tif'))
patch = Patch.createPatch(pZ, LMMosaicsPath)
layerZ.add(patch)
IJ.log('Setting the affineTransform ' + str(affTransform))
patch.setAffineTransform(affTransform)
patch.updateBucket()
bb = Rectangle(0, 0, widthEM, heightEM)
affineCroppedIm = loaderZ.getFlatImage(layerZ, bb, 1, 0x7fffffff,
ImagePlus.GRAY8, Patch,
layerZ.getAll(Patch), True,
Color.black, None)
affineCroppedImPath = os.path.join(
affineCroppedFolder,
'affineCropped_' + channel + '_' + str(l).zfill(4) + '.tif')
IJ.save(affineCroppedIm, affineCroppedImPath)
affineCroppedIm.close()
layerZ.remove(patch)
layerZ.recreateBuckets()
IJ.log('Has been written: ' + str(affineCroppedImPath))
fc.closeProject(pZ) # close dummy trakem
elem = l[:-1].split(" ")
fname = elem[0]
xloc = int(elem[1])
yloc = int(elem[2])
zloc = int(elem[3])
z_list.append(zloc)
imp = IJ.openImage(os.path.join(img_dir, fname))
patch = Patch(project, imp.title, xloc, yloc, imp)
patch.project.loader.addedPatchFrom(os.path.join(img_dir, fname),
patch)
layer = layerset.getLayer(zloc, 1, True)
layer.add(patch)
f.close()
front = Display.getFront()
bounds = Rectangle(x=0, y=0, width=20000, height=20000)
front.resizeCanvas(bounds)
z_list.sort()
if z_list[0] != 0:
layer = layerset.getLayer(0, 1, False)
layer.remove(False)
# Save project
project.saveAs(os.path.join(proj_dir, "montage_v1.xml"), False)
front.close(project)
def _java_screenshot(self, path):
size = Toolkit.getDefaultToolkit().getScreenSize()
rectangle = Rectangle(0, 0, size.width, size.height)
image = Robot().createScreenCapture(rectangle)
ImageIO.write(image, 'jpg', File(path))
layerset = areatree.getLayerSet()
calibration = layerset.getCalibration()
affine = areatree.getAffineTransform()
# outAndInArray = areatree.findConnectors()
for nd in root.getSubtreeNodes():
# get node coordinates, from tut on web
fp = array([nd.getX(), nd.getY()], 'f')
affine.transform(fp, 0, fp, 0, 1)
x = fp[0] * calibration.pixelWidth
y = fp[1] * calibration.pixelHeight
z = nd.getLayer().getZ(
) * calibration.pixelWidth # a TrakEM2 oddity
# get node connections, in/out synapse number, synapse id
# NOTE: this method seems to be affected by display zoom value
area = Area(Rectangle(int(fp[0]), int(fp[1]), 1, 1))
# area.transform(affine)
inAndOuts = layerset.findZDisplayables(Connector, nd.getLayer(),
area, False, False)
outgoing = []
incoming = []
for connector in inAndOuts:
if connector is None:
break
if connector.intersectsOrigin(area, nd.getLayer()):
outgoing.append(connector)
else:
incoming.append(connector)
nInputs = len(incoming)
nOutputs = len(outgoing)
outgoingIds = [oc.getId() for oc in outgoing]
from java.util.concurrent import Executors
from java.lang import Runtime
from java.awt import Rectangle
from net.imglib2.img.display.imagej import ImageJFunctions as IL
from ij import IJ
# Open Nile Bend sample image
imp = IJ.getImage()
#imp = IJ.openImage("https://imagej.nih.gov/ij/images/NileBend.jpg")
img = IL.wrapRGBA(imp)
# Extract red channel: alpha:0, red:1, green:2, blue:3
red = Converters.argbChannel(img, 1)
# Cut out two overlapping ROIs
r1 = Rectangle(1708, 680, 1792, 1760)
r2 = Rectangle(520, 248, 1660, 1652)
cut1 = Views.zeroMin(
Views.interval(red, [r1.x, r1.y],
[r1.x + r1.width - 1, r1.y + r1.height - 1]))
cut2 = Views.zeroMin(
Views.interval(red, [r2.x, r2.y],
[r2.x + r2.width - 1, r2.y + r2.height - 1]))
# Thread pool
exe = Executors.newFixedThreadPool(Runtime.getRuntime().availableProcessors())
try:
# PCM: phase correlation matrix
pcm = PhaseCorrelation2.calculatePCM(cut1, cut2,
ArrayImgFactory(FloatType()),
def run(self):
global filepath
gl.log("running MagicDrawAnimator2")
mode = 0
if mode == 0:
#filepath = "c:\\Users\\bclement\\git\\bae\\simulationSnapshot.Scenario_medium_2012.txt"
#filepath = "c:\\Users\\bclement\\Desktop\\large6.txt"
#filepath = "c:\\Users\\bclement\\Desktop\\foo12.txt"
#filepath = "/Users/mjackson/Desktop/testSim.txt"
gl.log("default filepath = %s" % filepath)
filepath = JOptionPane.showInputDialog(None,
"Select File to Animate",
"Select File",
JOptionPane.PLAIN_MESSAGE,
None, None, filepath)
gl.log("reading events from " + filepath)
self.events = []
try:
f = open(filepath, "r")
except:
gl.log("can't find file @ %s" % filepath)
return
lastTime = float(0.0)
latestTime = float(-1.0e20)
earliestTime = float(1.0e20)
gl.log(
"MagicDrawAnimator2: before simulation, loading events from " +
str(filepath))
print(
"MagicDrawAnimator2: before simulation, loading events from " +
str(filepath))
for line in f.readlines():
gl.log("read line = " + line)
x = re.search(
"(\d*)[^0-9: \t\n\r\f\v]*\s*:\s*\S*\s*(\S*) -> (\S*)\s*(\S*) ==>",
line)
y = re.search("(\S*) -> (\S*)\s*(\S*) ==>", line)
if x:
eventTime = float(x.groups()[0])
lastTime = eventTime
action = x.groups()[2]
cid = x.groups()[3]
ctype = x.groups()[1]
#gl.log("%s %s (%s)" % (action.upper(), cid, ctype))
elif y:
eventTime = lastTime
action = y.groups()[1]
cid = y.groups()[2]
ctype = y.groups()[0]
#gl.log("%s %s (%s)" % (action.upper(), cid, ctype))
elif line.startswith("---"):
gl.log(line)
continue
else:
continue
#gl.log("%s %s (%s)" % (action.upper(), cid, ctype))
if any([x in cid for x in ["Main"]]):
#gl.log(" ---> Skipping - can't animate Main")
continue
if re.search("(_)?Activity(_.*)?(?!\S)", ctype):
#gl.log(" ---> Skipping - can't animate the Activity object!")
continue
try:
if eventTime < earliestTime:
earliestTime = eventTime
if eventTime > latestTime:
latestTime = eventTime
evt = event(eventTime, action, cid, ctype)
try:
self.events.append(evt)
except:
gl.log("NESTED ARGHHHHH")
except:
gl.log("ARRGHHH")
print("MagicDrawAnimator2: finished loading events from " +
str(filepath))
gl.log("MagicDrawAnimator2: finished loading events from " +
str(filepath))
elementsNotEnded = []
try:
mda = MagicDrawAnimatorUtils2.MagicDrawAnimator2()
#self.playEvents(mda)
if self.timeScale == 0.0:
gl.log("*** ZERO TIMESCALE: setting timeScale to 1.0")
self.timeScale = 1.0
if usingDialogs:
JOptionPane.showMessageDialog(
self.frame, "Click ok to start simulation.")
#simulatedDuration = (float(latestTime) - float(earliestTime))/ float(self.timeScale)
simulatedDuration = (latestTime -
earliestTime) / self.timeScale
lastTime = earliestTime
elapsedTime = earliestTime
simStartTime = earliestTime / self.timeScale #start at time of first event
elapsedSimTime = simStartTime #start at time of first event
zeroTime = time.time()
except:
exceptionType, exceptionValue, exceptionTraceback = sys.exc_info(
)
gl.log("*** EXCEPTION:")
messages = traceback.format_exception(exceptionType,
exceptionValue,
exceptionTraceback)
for message in messages:
gl.log(message)
print("MagicDrawAnimator2: starting simulation with timeScale = " \
+ str(self.timeScale) + "; last event at " + str(latestTime) \
+ " scaling to " + str(simulatedDuration) + " seconds")
gl.log("MagicDrawAnimator2: starting simulation with timeScale = " \
+ str(self.timeScale) + "; last event at " + str(latestTime) \
+ " scaling to " + str(simulatedDuration) + " seconds")
try:
for evt in self.events:
#gl.log("EVENT at " + str(evt.eventTime))
#Sprint("EVENT at " + str(evt.eventTime))
try:
timeOfNextEvent = (float(evt.eventTime) +
0.0) / self.timeScale
except:
exceptionType, exceptionValue, exceptionTraceback = sys.exc_info(
)
gl.log("*** EXCEPTION:")
messages = traceback.format_exception(
exceptionType, exceptionValue, exceptionTraceback)
for message in messages:
gl.log(message)
#print("1")
if evt.eventTime != lastTime:
mda.doThePaint(
) #paint all events occurring at the previous event time
lastTime = float(evt.eventTime)
#print("2")
if timeOfNextEvent > elapsedSimTime:
print("sleeping sim time of next event(" + str(timeOfNextEvent) \
+ ") - elapsed sim time(" + str(elapsedSimTime) + ") = " \
+ str(timeOfNextEvent - elapsedSimTime) + " seconds")
gl.log("sleeping sim time of next event(" + str(timeOfNextEvent) \
+ ") - elapsed sim time(" + str(elapsedSimTime) + ") = " \
+ str(timeOfNextEvent - elapsedSimTime) + " seconds")
if (timeOfNextEvent - elapsedSimTime) > 300:
gl.log("found time jump > 300")
break
time.sleep(timeOfNextEvent - elapsedSimTime)
if "TimeVaryingMap" in evt.componentId:
try:
val = evt.action.split("=")[1]
except:
val = evt.action
gl.log(" ---> (%s) SETTING VALUE of %s: %s" %
(evt.eventTime, evt.componentType, val))
elif evt.componentType.startswith(
"sig") and "ObjectFlow" in evt.componentId:
sid = evt.componentType.strip("sig")
if "null" in evt.action and sid in elementsNotEnded:
gl.log(" ---> (%s) ENDING SIGNAL %s" %
(evt.eventTime, sid))
mda.end(sid)
elementsNotEnded.remove(sid)
elif "null" not in evt.action:
gl.log(" ---> (%s) STARTING SIGNAL %s ==> %s" %
(evt.eventTime, sid, evt.action))
txt = ""
try:
txt = evt.action.split("=")[1]
except:
txt = evt.action
mda.start(sid, txt)
if sid not in elementsNotEnded:
elementsNotEnded.append(sid)
elif "start" in evt.action:
gl.log(" ---> (%s) STARTING" % evt.eventTime)
mda.start(evt.componentId)
if evt.componentId not in elementsNotEnded:
elementsNotEnded.append(evt.componentId)
elif "end" in evt.action:
gl.log(" ---> (%s) ENDING" % evt.eventTime)
mda.end(evt.componentId)
if evt.componentId in elementsNotEnded:
elementsNotEnded.remove(evt.componentId)
t = time.time()
elapsedSimTime = t - zeroTime + simStartTime
elapsedTime = self.timeScale * elapsedSimTime
gl.log("Debug: elapsed time = " + str(elapsedSimTime))
except:
self.handleException()
if len(elementsNotEnded) > 0:
gl.log("Ending Still-Active Events:")
for e in elementsNotEnded:
gl.log(" ending %s " % e)
try:
mda.end(e)
except:
self.handleException()
mda.doThePaint()
else:
gl.log("All Events Completed")
gl.log("DONE")
elif mode == 1:
e = "_17_0_2_edc0357_1352328158277_34448_20209"
mda = MagicDrawAnimatorUtils2.MagicDrawAnimator2()
gl.log("Starting e (%s)" % e)
mda.start(e, "Porpoise Hork")
mda.doThePaint()
i = 3
while i > 0:
i -= 1
gl.log(".")
time.sleep(1)
gl.log("ending e")
mda.end(e)
mda.doThePaint()
elif mode == 2:
try:
mda = MagicDrawAnimatorUtils2.MagicDrawAnimator2()
e = "_17_0_2_edc0357_1352328158277_34448_20209" # in the usePower activity
gl.log("Starting e (%s)" % e)
mda.start(e)
mda.doThePaint()
tb = None
pem = PresentationElementsManager.getInstance()
sm = SessionManager.getInstance()
try:
sym = mda.gimmeTheSymbol(e)
midPoint = sym.getMiddlePoint()
sm.createSession("make box!")
try:
parentPM = sym.getPropertyManager()
c = Color.GREEN
tb = pem.createTextBox(sym, midPoint)
newBounds = Rectangle(15, 15)
newBounds.setLocation(midPoint)
pem.reshapeShapeElement(tb, newBounds)
pem.setText(tb, " gobblelkajdlfkjakj")
pem.setPresentationElementProperties(tb, parentPM)
sm.closeSession()
except:
sm.cancelSession()
self.handleException()
except:
self.handleException()
i = 3
while i > 0:
i -= 1
gl.log(".")
time.sleep(1)
gl.log("ending e")
mda.end(e)
if tb:
sm.createSession("deleteBox!")
try:
pem.deletePresentationElement(tb)
sm.closeSession()
except:
sm.cancelSession()
self.handleException()
mda.doThePaint()
except:
self.handleException()
def run(self):
global filepath
gl.log("running MagicDrawAnimator2")
mode = 0
if mode == 0:
#filepath = "c:\\Users\\bclement\\git\\bae\\simulationSnapshot.Scenario_medium_2012.txt"
#filepath = "c:\\Users\\bclement\\Desktop\\large6.txt"
#filepath = "c:\\Users\\bclement\\Desktop\\foo12.txt"
#filepath = "/Users/mjackson/Desktop/testSim.txt"
gl.log("default filepath = %s" % filepath)
filepath = JOptionPane.showInputDialog(
None,
"Select File to Animate",
"Select File",
JOptionPane.PLAIN_MESSAGE,
None,
None,
filepath)
gl.log("reading events from " + filepath)
self.events = []
try: f = open(filepath,"r")
except:
gl.log("can't find file @ %s" % filepath)
return
lastTime = float(0.0)
latestTime = float(-1.0e20)
earliestTime = float(1.0e20)
gl.log( "MagicDrawAnimator2: before simulation, loading events from " + str(filepath) )
print( "MagicDrawAnimator2: before simulation, loading events from " + str(filepath) )
for line in f.readlines():
gl.log("read line = " + line)
x = re.search("(\d*)[^0-9: \t\n\r\f\v]*\s*:\s*\S*\s*(\S*) -> (\S*)\s*(\S*) ==>",line)
y = re.search("(\S*) -> (\S*)\s*(\S*) ==>",line)
if x:
eventTime=float(x.groups()[0])
lastTime = eventTime
action=x.groups()[2]
cid = x.groups()[3]
ctype = x.groups()[1]
#gl.log("%s %s (%s)" % (action.upper(), cid, ctype))
elif y:
eventTime=lastTime
action=y.groups()[1]
cid = y.groups()[2]
ctype = y.groups()[0]
#gl.log("%s %s (%s)" % (action.upper(), cid, ctype))
elif line.startswith("---"):
gl.log(line)
continue
else: continue
#gl.log("%s %s (%s)" % (action.upper(), cid, ctype))
if any([x in cid for x in ["Main"]]):
#gl.log(" ---> Skipping - can't animate Main")
continue
if re.search("(_)?Activity(_.*)?(?!\S)",ctype):
#gl.log(" ---> Skipping - can't animate the Activity object!")
continue
try:
if eventTime < earliestTime:
earliestTime = eventTime
if eventTime > latestTime:
latestTime = eventTime
evt = event(eventTime,action,cid,ctype)
try: self.events.append(evt)
except: gl.log("NESTED ARGHHHHH")
except: gl.log("ARRGHHH")
print( "MagicDrawAnimator2: finished loading events from " + str(filepath) )
gl.log( "MagicDrawAnimator2: finished loading events from " + str(filepath) )
elementsNotEnded = []
try:
mda = MagicDrawAnimatorUtils2.MagicDrawAnimator2()
#self.playEvents(mda)
if self.timeScale == 0.0:
gl.log("*** ZERO TIMESCALE: setting timeScale to 1.0")
self.timeScale = 1.0
if usingDialogs:
JOptionPane.showMessageDialog(self.frame,"Click ok to start simulation.")
#simulatedDuration = (float(latestTime) - float(earliestTime))/ float(self.timeScale)
simulatedDuration = (latestTime - earliestTime)/ self.timeScale
lastTime = earliestTime
elapsedTime = earliestTime
simStartTime = earliestTime / self.timeScale #start at time of first event
elapsedSimTime = simStartTime #start at time of first event
zeroTime = time.time()
except:
exceptionType, exceptionValue, exceptionTraceback = sys.exc_info()
gl.log("*** EXCEPTION:")
messages=traceback.format_exception(exceptionType, exceptionValue, exceptionTraceback)
for message in messages:
gl.log(message)
print("MagicDrawAnimator2: starting simulation with timeScale = " \
+ str(self.timeScale) + "; last event at " + str(latestTime) \
+ " scaling to " + str(simulatedDuration) + " seconds")
gl.log("MagicDrawAnimator2: starting simulation with timeScale = " \
+ str(self.timeScale) + "; last event at " + str(latestTime) \
+ " scaling to " + str(simulatedDuration) + " seconds")
try:
for evt in self.events:
#gl.log("EVENT at " + str(evt.eventTime))
#Sprint("EVENT at " + str(evt.eventTime))
try: timeOfNextEvent = (float(evt.eventTime) + 0.0) / self.timeScale
except:
exceptionType, exceptionValue, exceptionTraceback = sys.exc_info()
gl.log("*** EXCEPTION:")
messages=traceback.format_exception(exceptionType, exceptionValue, exceptionTraceback)
for message in messages:
gl.log(message)
#print("1")
if evt.eventTime != lastTime:
mda.doThePaint() #paint all events occurring at the previous event time
lastTime = float(evt.eventTime)
#print("2")
if timeOfNextEvent > elapsedSimTime:
print("sleeping sim time of next event(" + str(timeOfNextEvent) \
+ ") - elapsed sim time(" + str(elapsedSimTime) + ") = " \
+ str(timeOfNextEvent - elapsedSimTime) + " seconds")
gl.log("sleeping sim time of next event(" + str(timeOfNextEvent) \
+ ") - elapsed sim time(" + str(elapsedSimTime) + ") = " \
+ str(timeOfNextEvent - elapsedSimTime) + " seconds")
if (timeOfNextEvent - elapsedSimTime) > 300:
gl.log("found time jump > 300")
break
time.sleep( timeOfNextEvent - elapsedSimTime )
if "TimeVaryingMap" in evt.componentId:
try: val = evt.action.split("=")[1]
except: val = evt.action
gl.log(" ---> (%s) SETTING VALUE of %s: %s" % (evt.eventTime,evt.componentType,val))
elif evt.componentType.startswith("sig") and "ObjectFlow" in evt.componentId:
sid = evt.componentType.strip("sig")
if "null" in evt.action and sid in elementsNotEnded:
gl.log(" ---> (%s) ENDING SIGNAL %s" % (evt.eventTime,sid))
mda.end(sid)
elementsNotEnded.remove(sid)
elif "null" not in evt.action:
gl.log(" ---> (%s) STARTING SIGNAL %s ==> %s" % (evt.eventTime,sid,evt.action))
txt = ""
try: txt = evt.action.split("=")[1]
except: txt = evt.action
mda.start(sid,txt)
if sid not in elementsNotEnded: elementsNotEnded.append(sid)
elif "start" in evt.action:
gl.log(" ---> (%s) STARTING" % evt.eventTime)
mda.start(evt.componentId)
if evt.componentId not in elementsNotEnded: elementsNotEnded.append(evt.componentId)
elif "end" in evt.action:
gl.log(" ---> (%s) ENDING" % evt.eventTime)
mda.end(evt.componentId)
if evt.componentId in elementsNotEnded: elementsNotEnded.remove(evt.componentId)
t = time.time()
elapsedSimTime = t - zeroTime + simStartTime
elapsedTime = self.timeScale * elapsedSimTime
gl.log("Debug: elapsed time = " + str(elapsedSimTime))
except: self.handleException()
if len(elementsNotEnded)>0:
gl.log("Ending Still-Active Events:")
for e in elementsNotEnded:
gl.log(" ending %s " % e)
try: mda.end(e)
except: self.handleException()
mda.doThePaint()
else: gl.log("All Events Completed")
gl.log("DONE")
elif mode == 1:
e = "_17_0_2_edc0357_1352328158277_34448_20209"
mda = MagicDrawAnimatorUtils2.MagicDrawAnimator2()
gl.log("Starting e (%s)" % e)
mda.start(e,"Porpoise Hork")
mda.doThePaint()
i = 3
while i > 0:
i-=1
gl.log(".")
time.sleep(1)
gl.log("ending e")
mda.end(e)
mda.doThePaint()
elif mode == 2:
try:
mda = MagicDrawAnimatorUtils2.MagicDrawAnimator2()
e = "_17_0_2_edc0357_1352328158277_34448_20209" # in the usePower activity
gl.log("Starting e (%s)" % e)
mda.start(e)
mda.doThePaint()
tb = None
pem = PresentationElementsManager.getInstance()
sm = SessionManager.getInstance()
try:
sym = mda.gimmeTheSymbol(e)
midPoint = sym.getMiddlePoint()
sm.createSession("make box!")
try:
parentPM = sym.getPropertyManager()
c = Color.GREEN
tb = pem.createTextBox(sym,midPoint)
newBounds = Rectangle(15,15)
newBounds.setLocation(midPoint)
pem.reshapeShapeElement(tb,newBounds)
pem.setText(tb," gobblelkajdlfkjakj")
pem.setPresentationElementProperties(tb, parentPM)
sm.closeSession()
except:
sm.cancelSession()
self.handleException()
except: self.handleException()
i = 3
while i > 0:
i-=1
gl.log(".")
time.sleep(1)
gl.log("ending e")
mda.end(e)
if tb:
sm.createSession("deleteBox!")
try:
pem.deletePresentationElement(tb)
sm.closeSession()
except:
sm.cancelSession()
self.handleException()
mda.doThePaint()
except: self.handleException()
def measure(stack, cells, nuclei):
time = [ (t-1)*cal.frameInterval for t in range(T+1) ]
cellValues0 = [ 0.0 for t in range(T+1) ]
cellValues1 = [ 0.0 for t in range(T+1) ]
cellAreas0 = [ 0.0 for t in range(T+1) ]
cellAreas1 = [ 0.0 for t in range(T+1) ]
nucleusValues0 = [ 0.0 for t in range(T+1) ]
nucleusValues1 = [ 0.0 for t in range(T+1) ]
nucleusAreas0 = [ 0.0 for t in range(T+1) ]
nucleusAreas1 = [ 0.0 for t in range(T+1) ]
nonNucleusValues0 = [ 0.0 for t in range(T+1) ]
nonNucleusValues1 = [ 0.0 for t in range(T+1) ]
for t in range(1,T+1):
ip = stack.getProcessor(t)
if cells[t] is None:
continue
#subtract background Z from all intensity Z measurements
if cells [t] is None:
print("Nocellsfound" + str(t))
bothCells = ShapeRoi(cells[t][0]).or(ShapeRoi(cells[t][1]))
backRoi = ShapeRoi(Rectangle(0,0,imp.getWidth(),imp.getHeight())).not( bothCells )
ip.setRoi(backRoi)
backMean = ip.getStatistics().mean
ip.setRoi( cells[t][0] )
stats0 = ip.getStatistics()
cellValues0[t] = stats0.mean - backMean
cellAreas0[t] = stats0.area * cal.pixelWidth * cal.pixelHeight
nuc0 = None
for nuc in nuclei[t]:
rect = nuc.getBounds()
nx = int(rect.x+(rect.width/2.0))
ny = int(rect.y+(rect.height/2.0))
if cells[t][0].contains(nx,ny):
nuc0 = nuc
break
if nuc0 is not None:
ip.setRoi( nuc0 )
nucStats0 = ip.getStatistics()
nucleusValues0[t] = nucStats0.mean - backMean
nucleusAreas0[t] = nucStats0.area * cal.pixelWidth * cal.pixelHeight
nuc0.setPosition(0,0,t)
nuc0.setStrokeColor(Color.CYAN)
ol.add(nuc0)
nonnucRoi0 = ShapeRoi(cells[t][0]).not( ShapeRoi(nuc0) )
ip.setRoi( nonnucRoi0 )
nonNucleusValues0[t] = ip.getStatistics().mean - backMean
ip.setRoi( cells[t][1] )
stats1 = ip.getStatistics()
cellValues1[t] = stats1.mean - backMean
cellAreas1[t] = stats1.area * cal.pixelWidth * cal.pixelHeight
nuc1 = None
for nuc in nuclei[t]:
rect = nuc.getBounds()
nx = int(rect.x+(rect.width/2.0))
ny = int(rect.y+(rect.height/2.0))
if cells[t][1].contains(nx,ny):
nuc1 = nuc
break
if nuc1 is not None:
ip.setRoi( nuc1 )
nucStats1 = ip.getStatistics()
nucleusValues1[t] = nucStats1.mean - backMean
nucleusAreas1[t] = nucStats1.area * cal.pixelWidth * cal.pixelHeight
nuc1.setPosition(0,0,t)
nuc1.setStrokeColor(Color.CYAN)
ol.add(nuc1)
nonnucRoi1 = ShapeRoi(cells[t][1]).not( ShapeRoi(nuc1) )
ip.setRoi( nonnucRoi1 )
nonNucleusValues1[t] = ip.getStatistics().mean - backMean
rt = ResultsTable()
rt.showRowNumbers(False)
for t in range(1,T+1):
rt.setValue("Time ("+cal.getTimeUnit()+")", t-1, IJ.d2s(time[t],1))
areaRatio = cellAreas0[t] / cellAreas1[t] if cellAreas0[t]>0 and cellAreas1[t]>0 else 0.0
rt.setValue("Cell 0:Cell 1 Area Ratio", t-1, areaRatio)
nucleusRatio = nucleusValues0[t] / nucleusValues1[t] if nucleusValues0[t]>0 and nucleusValues1[t]>0 else 0.0
rt.setValue("Cell 0:Cell 1 Nucleus Ratio", t-1, nucleusRatio)
nonNucleusRatio = nonNucleusValues0[t] / nonNucleusValues1[t] if nonNucleusValues0[t]>0 and nonNucleusValues1[t]>0 else 0.0
rt.setValue("Cell 0:Cell 1 Non-Nucleus Ratio", t-1, nonNucleusRatio)
nnnRatio0 = nucleusValues0[t] / nonNucleusValues0[t] if nucleusValues0[t]>0 and nonNucleusValues0[t]>0 else 0.0
rt.setValue("Cell 0 Nucleus:Non-Nucleus Ratio", t-1, nnnRatio0)
nnnRatio1 = nucleusValues1[t] / nonNucleusValues1[t] if nucleusValues1[t]>0 and nonNucleusValues1[t]>0 else 0.0
rt.setValue("Cell 1 Nucleus:Non-Nucleus Ratio", t-1, nnnRatio1)
rt.setValue("Cell 0 (red) Area ("+cal.getUnit()+u"\u00b2"+")", t-1, cellAreas0[t])
rt.setValue("Cell 0 Nucleus Area ("+cal.getUnit()+u"\u00b2"+")", t-1, nucleusAreas0[t])
rt.setValue("Cell 0 All", t-1, cellValues0[t])
rt.setValue("Cell 0 Nucleus", t-1, nucleusValues0[t])
rt.setValue("Cell 0 Non-Nucleus", t-1, nonNucleusValues0[t])
rt.setValue("Cell 1 (green) Area ("+cal.getUnit()+u"\u00b2"+")", t-1, cellAreas1[t])
rt.setValue("Cell 1 Nucleus Area ("+cal.getUnit()+u"\u00b2"+")", t-1, nucleusAreas1[t])
rt.setValue("Cell 1 All", t-1, cellValues1[t])
rt.setValue("Cell 1 Nucleus", t-1, nucleusValues1[t])
rt.setValue("Cell 1 Non-Nucleus", t-1, nonNucleusValues1[t])
rt.show(imp.getTitle()+"-Results")
dataset = DefaultXYDataset()
dataset.addSeries( "Cell 0", [time[1:], cellValues0[1:]] )
dataset.addSeries( "Cell 1", [time[1:], cellValues1[1:]] )
dataset.addSeries( "Nucleus 0", [time[1:], nucleusValues0[1:]] )
dataset.addSeries( "Nucleus 1", [time[1:], nucleusValues1[1:]] )
dataset.addSeries( "Non-Nucleus 0", [time[1:], nonNucleusValues0[1:]] )
dataset.addSeries( "Non-Nucleus 1", [time[1:], nonNucleusValues1[1:]] )
chart = ChartFactory.createScatterPlot( imp.getTitle(), "Time ("+cal.getTimeUnit()+")", "Intensity Z", dataset, PlotOrientation.VERTICAL, True,True,False )
plot = chart.getPlot()
plot.setBackgroundPaint(Color(64, 128, 255))
plot.setDomainGridlinePaint(Color.BLACK)
plot.setRangeGridlinePaint(Color.BLACK)
renderer = plot.getRenderer()
legend = LegendItemCollection()
shapeR = 2.0
nucShape = Ellipse2D.Float(-shapeR,-shapeR,shapeR*2,shapeR*2)
nonNucShape = Path2D.Float()
nonNucShape.moveTo(-shapeR,-shapeR)
nonNucShape.lineTo(shapeR,shapeR)
nonNucShape.moveTo(shapeR,-shapeR)
nonNucShape.lineTo(-shapeR,shapeR)
for s in range(dataset.getSeriesCount()):
if s == 0:
renderer.setSeriesLinesVisible(s, True)
renderer.setSeriesShapesVisible(s, False)
renderer.setSeriesStroke(s, BasicStroke(1))
renderer.setSeriesPaint(s, Color.RED)
legend.add( LegendItem("Cell 0", Color.RED) )
elif s == 1:
renderer.setSeriesLinesVisible(s, True)
renderer.setSeriesShapesVisible(s, False)
renderer.setSeriesStroke(s, BasicStroke(1))
renderer.setSeriesPaint(s, Color.GREEN)
legend.add( LegendItem("Cell 1", Color.GREEN) )
elif s == 2:
renderer.setSeriesLinesVisible(s, False)
renderer.setSeriesShapesVisible(s, True)
renderer.setSeriesShape(s, nucShape)
renderer.setSeriesPaint(s, Color.RED)
elif s == 3:
renderer.setSeriesLinesVisible(s, False)
renderer.setSeriesShapesVisible(s, True)
renderer.setSeriesShape(s, nucShape)
renderer.setSeriesPaint(s, Color.GREEN)
elif s == 4:
renderer.setSeriesLinesVisible(s, False)
renderer.setSeriesShapesVisible(s, True)
renderer.setSeriesShape(s, nonNucShape)
renderer.setSeriesPaint(s, Color.RED)
elif s == 5:
renderer.setSeriesLinesVisible(s, False)
renderer.setSeriesShapesVisible(s, True)
renderer.setSeriesShape(s, nonNucShape)
renderer.setSeriesPaint(s, Color.GREEN)
plot.setFixedLegendItems(legend)
frame = ChartFrame(imp.getTitle()+" Z-Normalised Intensity", chart)
frame.pack()
frame.setSize( Dimension(800, 800) )
frame.setLocationRelativeTo(None)
frame.setVisible(True)
def union(self, rect):
"""
Return Rect representing the union of rect and this rect.
"""
r = Rectangle.union(self, rect)
return Rect(r.x, r.y, r.width, r.height)
def __init__(self):
Rectangle.__init__(self, 6, 7)
def diagrambuttonPressed(event) :
IJ.showMessage("Push 'Auto' button each time you want to see the diagram")
x1=10
y1=20
x2=100
y2=50
x3=60
y3=30
xr=10
yr=20
wr=20
hr=20
rect=Rectangle(xr,yr,wr,hr)
#img=IJ.getImage()
#nbslices=self.__img.getImageStackSize()
nbslices=self.__maxLife
IJ.run("Hyperstack...", "title=Diagram type=32-bit display=Color width="+str(x2+(nbslices+1)*x3)+" height="+str(y2+y3*len(dico))+" channels=1 slices="+str(len(self.__measures))+" frames=1")
im=IJ.getImage()
ip=im.getProcessor()
for i in range(len(self.__measures)) :
indiceligne=0
maxvalue=0
minvalue=1000000
im.setPosition(1,i+1,1)
for cellname in self.__listcellname :
indiceligne+=1
for indicecolonne in range(1,nbslices+1):
rect.setLocation(x2+indicecolonne*x3+int(x3/6),(y1+indiceligne*y3-int(y3/2)))
# we create at the first iteration a dictionary with the rectangles (for a future use)
if i==0 :
self.__gridrectangle[(indiceligne,indicecolonne)]=Rectangle(rect)
im.setRoi(rect)
ipr=im.getProcessor()
# we find the min and max values of the datas for a measure given.
if self.__dictMeasures[dico[cellname]][self.__measures[i]][indicecolonne-1]>maxvalue :
maxvalue=self.__dictMeasures[dico[cellname]][self.__measures[i]][indicecolonne-1]
if self.__dictMeasures[dico[cellname]][self.__measures[i]][indicecolonne-1]<minvalue :
minvalue=self.__dictMeasures[dico[cellname]][self.__measures[i]][indicecolonne-1]
# we fill the rectangle with the value of the measure
ipr.setValue(self.__dictMeasures[dico[cellname]][self.__measures[i]][indicecolonne-1])
ipr.fill()
# we write the names and the n of slices on the image with the maxvalue.
ip.setValue(maxvalue)
ip.moveTo(x1,y1)
ip.drawString(self.__measures[i])
for j in range(1,nbslices+1) :
ip.moveTo(x2+j*x3,y1)
ip.drawString("Slice "+str(j))
j=0
for cellname in self.__listcellname :
ip.moveTo(x1,y2+j*y3)
ip.drawString(cellname)
j+=1
im.killRoi()
im=IJ.run(im,"Fire","")
IJ.run("Brightness/Contrast...", "")
#im.setMinAndMax(minvalue,maxvalue)
#im.updateImage()
#we add a mouse listener in order to be able to show the roi corresponding to a rectangle when the user clicks on it.
listener = ML()
listener.name=imgName
for imp in map(WindowManager.getImage, WindowManager.getIDList()):
if imp.getTitle().startswith("Diagram") :
win = imp.getWindow()
if win is None:
continue
win.getCanvas().addMouseListener(listener)
def __init__(self):
Rectangle.__init__(self, 6, 7)
def computeRegistration():
while atomicI.get() < nSections:
l = atomicI.getAndIncrement()
if l < nSections:
IJ.log('Computing EM/LM registration for layer ' + str(l).zfill(4))
layerFolder = fc.mkdir_p(
os.path.join(registrationFolder, 'layer_' + str(l).zfill(4)))
toRegisterFolder = fc.mkdir_p(
os.path.join(layerFolder, 'toRegister'))
registeredFolder = fc.mkdir_p(
os.path.join(layerFolder, 'registered'))
# Applying appropriate filters to make lowresEM and LM look similar for layer l
imLM = IJ.openImage(imPaths['LM'][l])
imLM = fc.localContrast(imLM)
imLMPath = os.path.join(toRegisterFolder,
'imLM_' + str(l).zfill(4) + '.tif')
IJ.save(imLM, imLMPath)
imEM = IJ.openImage(imPaths['EM'][l])
imEM = fc.localContrast(imEM)
imEM = fc.blur(imEM, 2)
imEMPath = os.path.join(toRegisterFolder,
'imEM_' + str(l).zfill(4) + '.tif')
IJ.save(imEM, imEMPath)
# Compute first a simple affine registration on the non-cropped images
IJ.log(
'Computing affine and moving least squares alignment for layer '
+ str(l).zfill(4))
firstStepRegistered = False
# registration at first step with 1step/octave (less features)
pLowRes = getSIFTMatchingParameters(nOctaves[0], 1.6, 16, 4000, 8,
4)
featuresLM = getFeatures(imLMPath, pLowRes)
featuresEM = getFeatures(imEMPath, pLowRes)
matchingResults = getMatchingResults(featuresLM, featuresEM)
if matchingResults is None:
IJ.log(
'No registration matching at low resolution matching step 1 in layer '
+ str(l).zfill(4))
else:
model, inliers = matchingResults
distance = PointMatch.meanDistance(
inliers
) # mean displacement of the remaining matching features
IJ.log('---Layer ' + str(l).zfill(4) + ' distance ' +
str(distance) + ' px with ' + str(len(inliers)) +
' inliers')
if distance > matchingThreshold[0]:
IJ.log(
'Matching accuracy is lower than the threshold at the low resolution step 1 - '
+ str(l).zfill(4) + ' - distance - ' + str(distance))
else:
affTransform = model.createAffine()
s1, s2 = getScalingFactors(affTransform)
IJ.log('Layer ' + str(l).zfill(4) +
' scaling factors - step 1 - ' + str(s1) + ' - ' +
str(s2) + '--' + str(s1 * s2) + ' affDeterminant ' +
str(affTransform.getDeterminant()) + ' nInliers ' +
str(len(inliers)))
if (abs(s1 - 1) < 0.2) and (
abs(s2 - 1) < 0.2
): # scaling in both directions should be close to 1
IJ.log('First step ok - layer ' + str(l).zfill(4))
firstStepRegistered = True
loaderZ.serialize(
affTransform,
os.path.join(registeredFolder, 'affineSerialized'))
if not firstStepRegistered:
IJ.log(
'First step registration in layer ' + str(l).zfill(4) +
' with few features has failed. Trying with more features.'
)
# registration at first step with 3steps/octave (more features)
pLowRes = getSIFTMatchingParameters(3, 1.6, 64, 4000, 8, 4)
#pLowRes = getSIFTMatchingParameters(nOctaves[0], 1.6, 16, 4000, 8, 4) # for BIB
featuresLM = getFeatures(imLMPath, pLowRes)
featuresEM = getFeatures(imEMPath, pLowRes)
matchingResults = getMatchingResults(featuresLM, featuresEM)
if matchingResults is None:
IJ.log(
'No registration matching at low resolution matching step 1bis in layer '
+ str(l).zfill(4))
else:
model, inliers = matchingResults
distance = PointMatch.meanDistance(
inliers
) # mean displacement of the remaining matching features
IJ.log('---Layer ' + str(l).zfill(4) + ' distance ' +
str(distance) + ' px with ' + str(len(inliers)) +
' inliers')
if distance > matchingThreshold[0]:
IJ.log(
'Matching accuracy is lower than the threshold at the high resolution step 1bis - '
+ str(l).zfill(4) + ' - distance - ' +
str(distance))
else:
affTransform = model.createAffine()
s1, s2 = getScalingFactors(affTransform)
IJ.log('Layer ' + str(l).zfill(4) +
' scaling factors - step 1bis - ' + str(s1) +
' - ' + str(s2) + '--' + str(s1 * s2) +
' affDeterminant ' +
str(affTransform.getDeterminant()) +
' nInliers ' + str(len(inliers)))
if (abs(s1 - 1) < 0.2) and (
abs(s2 - 1) < 0.2
): # scaling in both directions should be close to 1
IJ.log('First step 1bis ok - layer ' +
str(l).zfill(4))
firstStepRegistered = True
loaderZ.serialize(
affTransform,
os.path.join(registeredFolder,
'affineSerialized'))
if not firstStepRegistered:
IJ.log('The two first step trials in layer ' +
str(l).zfill(4) + ' have failed')
else:
# Affine transform and crop the LM, and compute a high res MLS matching
with lock: # only one trakem working at a time
# apply affTransform
patch = Patch.createPatch(pZ, imLMPath)
layerZ.add(patch)
patch.setAffineTransform(affTransform)
patch.updateBucket()
# crop and export
bb = Rectangle(0, 0, widthEM, heightEM)
affineCroppedIm = loaderZ.getFlatImage(
layerZ, bb, 1, 0x7fffffff, ImagePlus.GRAY8, Patch,
layerZ.getAll(Patch), True, Color.black, None)
affineCroppedImPath = os.path.join(
toRegisterFolder,
'affineCroppedLM_' + str(l).zfill(4) + '.tif')
IJ.save(affineCroppedIm, affineCroppedImPath)
affineCroppedIm.close()
layerZ.remove(patch)
layerZ.recreateBuckets()
pHighRes = getSIFTMatchingParameters(nOctaves[1], 1.6, 64,
4096, 8, 4)
featuresLM = getFeatures(affineCroppedImPath, pHighRes)
featuresEM = getFeatures(imEMPath, pHighRes)
# get the MLS
matchingResults = getMatchingResults(featuresLM, featuresEM)
if matchingResults is None:
IJ.log(
'It cannot be, there should be a good match given that an affine was computed. Layer '
+ str(l).zfill(4))
else:
model, inliers = matchingResults
affTransform = model.createAffine()
s1, s2 = getScalingFactors(affTransform)
IJ.log('Second step determinant - layer ' +
str(l).zfill(4) + ' - determinant - ' +
str(affTransform.getDeterminant()) + ' nInliers ' +
str(len(inliers)) + 'Scaling factors - step 2 - ' +
str(s1) + ' - ' + str(s2))
if (abs(s1 - 1) < 0.2) and (abs(s2 - 1) < 0.2) and len(
inliers
) > 50: # scaling in both directions should be close to 1
distance = PointMatch.meanDistance(
inliers
) # mean displacement of the remaining matching features
if distance > matchingThreshold[1]:
IJ.log(
'Weird: matching accuracy is lower than the threshold at the high resolution step 2 - '
+ str(l).zfill(4) + ' - distance - ' +
str(distance))
else:
mlst = MovingLeastSquaresTransform()
mlst.setModel(AffineModel2D)
mlst.setAlpha(1)
mlst.setMatches(inliers)
xmlMlst = mlst.toXML('\t')
with open(
os.path.join(registeredFolder, 'MLST.xml'),
'w') as f:
f.write(xmlMlst)
loaderZ.serialize(
mlst,
os.path.join(registeredFolder,
'mlstSerialized'))
registrationStats.append(
[l, distance, len(inliers)])