def posmap(args):
"""
%prog posmap frgscf.sorted scf.fasta scfID
Perform QC on the selected scfID, generate multiple BED files for plotting.
"""
p = OptionParser(posmap.__doc__)
opts, args = p.parse_args(args)
if len(args) != 3:
sys.exit(p.print_help())
frgscffile, fastafile, scf = args
# fasta
cmd = "faOneRecord {0} {1}".format(fastafile, scf)
scffastafile = scf + ".fasta"
if not op.exists(scffastafile):
sh(cmd, outfile=scffastafile)
# sizes
sizesfile = scffastafile + ".sizes"
sizes = Sizes(scffastafile).mapping
scfsize = sizes[scf]
logging.debug("`{0}` has length of {1}.".format(scf, scfsize))
# gaps.bed
gapsbedfile = scf + ".gaps.bed"
if not op.exists(gapsbedfile):
args = [scffastafile, "--bed", "--mingap=100"]
gaps(args)
# reads frgscf posmap
posmapfile = scf + ".posmap"
if not op.exists(posmapfile):
args = [frgscffile, scf]
query(args)
# reads bed
bedfile = scf + ".bed"
if not op.exists(bedfile):
args = [posmapfile]
bed(args)
# reads bedpe
bedpefile = scf + ".bedpe"
pairsbedfile = scf + ".pairs.bed"
if not (op.exists(bedpefile) and op.exists(pairsbedfile)):
bed_to_bedpe(bedfile, bedpefile, pairsbedfile=pairsbedfile, ca=True)
# base coverage
basecoverage = Coverage(bedfile, sizesfile)
pecoverage = Coverage(pairsbedfile, sizesfile)
def posmap(args):
"""
%prog posmap frgscf.sorted scf.fasta scfID
Perform QC on the selected scfID, generate multiple BED files for plotting.
"""
p = OptionParser(posmap.__doc__)
opts, args = p.parse_args(args)
if len(args) != 3:
sys.exit(p.print_help())
frgscffile, fastafile, scf = args
# fasta
cmd = "faOneRecord {0} {1}".format(fastafile, scf)
scffastafile = scf + ".fasta"
if not op.exists(scffastafile):
sh(cmd, outfile=scffastafile)
# sizes
sizesfile = scffastafile + ".sizes"
sizes = Sizes(scffastafile).mapping
scfsize = sizes[scf]
logging.debug("`{0}` has length of {1}.".format(scf, scfsize))
# gaps.bed
gapsbedfile = scf + ".gaps.bed"
if not op.exists(gapsbedfile):
args = [scffastafile, "--bed", "--mingap=100"]
gaps(args)
# reads frgscf posmap
posmapfile = scf + ".posmap"
if not op.exists(posmapfile):
args = [frgscffile, scf]
query(args)
# reads bed
bedfile = scf + ".bed"
if not op.exists(bedfile):
args = [posmapfile]
bed(args)
# reads bedpe
bedpefile = scf + ".bedpe"
pairsbedfile = scf + ".pairs.bed"
if not (op.exists(bedpefile) and op.exists(pairsbedfile)):
bed_to_bedpe(bedfile, bedpefile, pairsbedfile=pairsbedfile, ca=True)
# base coverage
Coverage(bedfile, sizesfile)
Coverage(pairsbedfile, sizesfile)