class ContextHandlerTest(unittest.TestCase):
def setUp(self):
self.protocol = Protocol()
def assertVolume(self, well, volume):
result = self.protocol._context_handler.get_volume(well)
self.assertEqual(volume, result)
def test_transfer(self):
""" Maintain well volumes during transfers. """
self.protocol.add_container('A1', 'microplate.96')
self.protocol.add_container('C1', 'tiprack.p200')
self.protocol.add_instrument('A', 'p200')
self.protocol.calibrate('A1', x=1, y=2, z=3)
self.assertVolume('A1:A2', 0)
self.protocol.transfer('A1:A1', 'A1:A2', ul=100)
self.assertVolume('A1:A2', 100)
self.protocol.transfer('A1:A2', 'A1:A3', ul=20)
self.assertVolume('A1:A3', 20)
self.assertVolume('A1:A2', 80)
run = self.protocol.run()
next(run) # Yield to set progress.
self.assertVolume('A1:A2', 0)
next(run) # transfer('A1:A1', 'A1:A2', ul=100)
self.assertVolume('A1:A2', 100)
next(run) # transfer('A1:A2', 'A1:A3', ul=20)
self.assertVolume('A1:A3', 20)
self.assertVolume('A1:A2', 80)
def test_distribute(self):
self.protocol.add_container('A1', 'microplate.96')
self.protocol.add_container('C1', 'tiprack.p200')
self.protocol.add_instrument('A', 'p200')
self.protocol.distribute(
'A1:A1',
('A1:B1', {'ul': 50}),
('A1:C1', {'ul': 30}),
('A1:D1', {'ul': 40})
)
# Final volumes.
self.assertVolume('A1:A1', -120)
self.assertVolume('A1:B1', 50)
self.assertVolume('A1:C1', 30)
self.assertVolume('A1:D1', 40)
# Try during a run.
run = self.protocol.run()
next(run) # Yield to set progress.
self.assertVolume('A1:A2', 0)
next(run) # Our command.
# Final volumes
self.assertVolume('A1:A1', -120)
self.assertVolume('A1:B1', 50)
self.assertVolume('A1:C1', 30)
self.assertVolume('A1:D1', 40)
def test_consolidate(self):
self.protocol.add_container('A1', 'microplate.96')
self.protocol.add_instrument('A', 'p200')
self.protocol.consolidate(
'A1:A1',
('A1:B1', {'ul': 50}),
('A1:C1', {'ul': 30}),
('A1:D1', {'ul': 40})
)
self.assertVolume('A1:A1', 120)
self.assertVolume('A1:B1', -50)
self.assertVolume('A1:C1', -30)
self.assertVolume('A1:D1', -40)
def test_transfer_group(self):
self.protocol.add_container('A1', 'microplate.96')
self.protocol.add_container('C1', 'tiprack.p200')
self.protocol.add_instrument('A', 'p200')
self.protocol.transfer_group(
('A1:A1', 'A1:B1', {'ul': 50}),
('A1:A1', 'A1:C1', {'ul': 50}),
('A1:A1', 'A1:D1', {'ul': 30}),
tool='p200'
)
self.assertVolume('A1:A1', -130)
self.assertVolume('A1:B1', 50)
self.assertVolume('A1:C1', 50)
self.assertVolume('A1:D1', 30)
def test_find_instrument_by_volume(self):
""" Find instrument by volume. """
self.protocol.add_instrument('A', 'p10')
i = self.protocol._context_handler.get_instrument(has_volume=6)
self.assertEqual(i.supports_volume(6), True)
j = self.protocol._context_handler.get_instrument(has_volume=50)
self.assertEqual(j, None)
self.protocol.add_instrument('B', 'p200')
k = self.protocol._context_handler.get_instrument(has_volume=50)
self.assertEqual(k.name, 'p200')
def test_tip_coordinates(self):
""" Return tip coordinates. """
context = self.protocol._context_handler
self.protocol.add_instrument('A', 'p200')
self.protocol.add_instrument('B', 'p10')
self.protocol.add_container('B1', 'tiprack.p200')
self.protocol.calibrate('B1', axis="A", x=100, y=150, top=60)
self.protocol.add_container('A1', 'tiprack.p10')
self.protocol.calibrate('A1', axis="B", x=200, y=250, top=160)
p200 = context.get_instrument(axis='A')
p10 = context.get_instrument(axis='B')
c1 = context.get_next_tip_coordinates(p200)
self.assertEqual(c1, {'x': 100, 'y': 150, 'top': 60, 'bottom': 0})
c2 = context.get_next_tip_coordinates(p200) # Next tip.
self.assertEqual(c2, {'x': 100, 'y': 159, 'top': 60, 'bottom': 0})
c3 = context.get_next_tip_coordinates(p10)
self.assertEqual(c3, {'x': 200, 'y': 250, 'top': 160, 'bottom': 0})
c4 = context.get_next_tip_coordinates(p10) # Next tip.
self.assertEqual(c4, {'x': 200, 'y': 259, 'top': 160, 'bottom': 0})
def test_tiprack_switch(self):
""" Return second tiprack when first is used up. """
context = self.protocol._context_handler
self.protocol.add_instrument('A', 'p200')
self.protocol.add_container('B1', 'tiprack.p200')
self.protocol.calibrate('B1', axis="A", x=100, y=150, top=60)
self.protocol.add_container('A1', 'tiprack.p200')
self.protocol.calibrate('A1', axis="A", x=200, y=250, top=160)
p200 = context.get_instrument(axis='A')
rack = context.find_container(name='tiprack.p200', has_tips=True)
self.assertEqual([(0, 0)], rack.address)
rack.set_tips_used(95) # We've used all but one tip from this rack.
c1 = context.get_next_tip_coordinates(p200) # Last tip.
h12 = [(0, 0), (7, 11)]
self.assertEqual(c1, context.get_coordinates(h12, axis="A"))
rack = context.find_container(name='tiprack.p200', has_tips=True)
self.assertEqual([(1, 0)], rack.address)
def test_multichannel_search(self):
""" Find a multichannel pipette. """
context = self.protocol._context_handler
self.protocol.add_instrument('A', 'p200')
self.protocol.add_instrument('B', 'p20.12')
i1 = context.get_instrument(axis='B')
i2 = context.get_instrument(has_volume=20, channels=12)
self.assertEqual(i1, i2)
i3 = context.get_instrument(has_volume=200, channels=12)
self.assertEqual(i3, None)
def test_multichannel_tip_allocation(self):
context = self.protocol._context_handler
self.protocol.add_instrument('A', 'p20.12')
self.protocol.add_instrument('B', 'p20.8')
self.protocol.add_container('A1', 'tiprack.p20')
a = context.get_instrument(axis='A')
b = context.get_instrument(axis='B')
self.protocol.calibrate('A1', axis="A")
self.protocol.calibrate('A1', axis="B")
# Get a row first.
context.get_next_tip_coordinates(a)
with self.assertRaises(x.TipMissing):
context.get_next_tip_coordinates(b)
# Get a col first.
context = self.protocol.initialize_context()
context.get_next_tip_coordinates(b)
with self.assertRaises(x.TipMissing):
context.get_next_tip_coordinates(a)
# Add another tiprack, get both!
self.protocol.add_container('A2', 'tiprack.p20')
self.protocol.calibrate('A2', axis="A")
self.protocol.calibrate('A2', axis="B")
context = self.protocol.initialize_context()
context.get_next_tip_coordinates(a)
context.get_next_tip_coordinates(b)
# Exhaust the supply.
context = self.protocol.initialize_context()
for _ in range(8):
context.get_next_tip_coordinates(a)
for _ in range(12):
context.get_next_tip_coordinates(b)
with self.assertRaises(x.TipMissing):
context.get_next_tip_coordinates(a)
with self.assertRaises(x.TipMissing):
context.get_next_tip_coordinates(b)
def test_multichannel_transfer_cols(self):
""" Test multichannel transfer (cols). """
self.protocol.add_instrument('A', 'p20.12')
self.protocol.add_instrument('B', 'p20.8')
self.protocol.add_container('A1', 'microplate')
p = self.protocol._context_handler.find_container(name="microplate")
self.protocol.transfer('A1:A1', 'A1:B1', ul=10, tool='p20.12')
self.assertEqual(p.col('A').get_volume(), [-10 for n in range(12)])
self.assertEqual(p.col('B').get_volume(), [ 10 for n in range(12)])
def test_multichannel_transfer_rows(self):
""" Test multichannel transfer (rows). """
self.protocol.add_instrument('A', 'p20.12')
self.protocol.add_instrument('B', 'p20.8')
self.protocol.add_container('A1', 'microplate')
p = self.protocol._context_handler.find_container(name="microplate")
self.protocol.transfer('A1:A1', 'A1:A2', ul=15, tool='p20.8')
self.assertEqual(p.row(0).get_volume(), [-15 for n in range(8)])
self.assertEqual(p.row(1).get_volume(), [ 15 for n in range(8)])
class MotorHandlerTest(unittest.TestCase):
def setUp(self):
self.protocol = Protocol()
def test_basic_transfer(self):
""" Basic transfer. """
motor = self.protocol.attach_motor()
output_log = motor._driver
self.protocol.add_instrument('A', 'p20')
self.protocol.add_instrument('B', 'p200')
self.protocol.add_container('A1', 'microplate.96')
self.protocol.add_container('C1', 'tiprack.p200')
self.protocol.add_container('B1', 'tiprack.p20')
self.protocol.add_container('B2', 'point.trash')
self.protocol.calibrate('A1', axis="A", x=1, y=2, top=3, bottom=10)
self.protocol.calibrate('B1', axis="A", x=4, y=5, top=6, bottom=15)
self.protocol.calibrate('B2', axis="A", x=50, y=60, top=70)
self.protocol.calibrate('A1:A2', axis="B", bottom=5)
self.protocol.calibrate('C1', axis="B", x=100, y=100, top=50)
self.protocol.calibrate('B2', axis="B", x=200, y=250, top=15)
self.protocol.calibrate('A1', axis="B", x=1, y=2, top=3, bottom=13)
self.protocol.calibrate_instrument('A', top=0, blowout=1, droptip=2)
self.protocol.calibrate_instrument('B', top=0, blowout=10, droptip=25)
self.protocol.transfer('A1:A1', 'A1:A2', ul=10)
self.protocol.transfer('A1:A2', 'A1:A3', ul=80)
prog_out = []
for progress in self.protocol.run():
prog_out.append(progress)
expected = [
# Transfer 1.
{'x': 4, 'y': 5}, # Pickup tip.
{'z': 6},
{'z': 0}, # Move to well.
{'x': 1, 'y': 2},
{'z': 3},
{'a': 0.5}, # Plunge.
{'x': 1, 'y': 2},
{'z': 10}, # Move into well.
{'a': 0}, # Release.
{'z': 0}, # Move up.
{'x': 1, 'y': 11}, # Move to well.
{'z': 3},
{'x': 1, 'y': 11},
{'z': 10}, # Move into well.
{'a': 1}, # Blowout.
{'z': 0}, # Move up.
{'a': 0}, # Release.
{'x': 50, 'y': 60}, # Dispose tip.
{'z': 70},
{'a': 2},
{'a': 0},
# Transfer 2.
{'x': 100, 'y': 100},
{'z': 50},
{'z': 0},
{'x': 1, 'y': 11},
{'z': 3},
{'b': 4.0},
{'x': 1, 'y': 11},
{'z': 5},
{'b': 0},
{'z': 0},
{'x': 1, 'y': 20},
{'z': 3},
{'x': 1, 'y': 20},
{'z': 13},
{'b': 10},
{'z': 0},
{'b': 0},
{'x': 200, 'y': 250},
{'z': 15},
{'b': 25},
{'b': 0}
]
self.assertEqual(expected, output_log.movements)
self.assertEqual([(0, 2), (1, 2), (2, 2)], prog_out)
def test_calibrate_without_axis(self):
self.protocol.add_instrument('A', 'p20')
self.protocol.add_instrument('B', 'p200')
with self.assertRaises(x.DataMissing):
self.protocol.calibrate('A1', top=0, bottom=0)
def test_transfer_without_tiprack(self):
""" Raise error when no tiprack found. """
self.protocol.attach_motor()
self.protocol.add_instrument('B', 'p200')
self.protocol.add_container('A1', 'microplate.96')
self.protocol.calibrate('A1', top=0, bottom=0)
self.protocol.calibrate_instrument('B', top=0, blowout=10)
self.protocol.transfer('A1:A1', 'A1:A2', ul=100)
with self.assertRaises(x.ContainerMissing):
self.protocol.run_all()
def test_transfer_without_dispose_point(self):
""" Raise when no dispose point set. """
self.protocol.attach_motor()
self.protocol.add_instrument('B', 'p200')
self.protocol.add_container('A1', 'microplate.96')
self.protocol.add_container('C1', 'tiprack.p200')
self.protocol.calibrate('A1')
self.protocol.calibrate('C1')
self.protocol.calibrate_instrument('B', top=0, blowout=10)
self.protocol.transfer('A1:A1', 'A1:A2', ul=100)
self.protocol.transfer('A1:A2', 'A1:A3', ul=80)
with self.assertRaises(x.ContainerMissing):
self.protocol.run_all()
def test_instrument_missing(self):
with self.assertRaises(x.InstrumentMissing):
m = self.protocol.attach_motor()
m.get_pipette(has_volume=1000)
def test_no_errors_from_commands(self):
self.protocol.add_instrument('B', 'p200')
self.protocol.add_container('A1', 'microplate.96')
self.protocol.add_container('B1', 'tiprack.p200')
self.protocol.add_container('C1', 'point.trash')
self.protocol.calibrate('A1')
self.protocol.calibrate('B1')
self.protocol.calibrate('C1')
self.protocol.calibrate('A1', x=1, y=2, top=3, bottom=13)
self.protocol.calibrate_instrument('B', top=0, blowout=10, droptip=25)
self.protocol.transfer('A1:A1', 'A1:A2', ul=100)
self.protocol.transfer_group(
('A1:A1', 'A1:A2', {'ul': 100})
)
self.protocol.consolidate(
'A1:A1',
('A1:A2', {'ul':100}),
('A1:A2', {'ul':150})
)
self.protocol.distribute(
'A1:A1',
('A1:A2', {'ul':100}),
('A1:A2', {'ul':150})
)
self.protocol.mix('A1:A2', ul=100, repetitions=5)
motor = self.protocol.attach_motor()
output_log = motor._driver
movements = output_log.movements
# We're not really testing anything except that it runs without
# errors.
self.protocol.run_all()
class ContextHandlerTest(unittest.TestCase):
def setUp(self):
self.protocol = Protocol()
def assertVolume(self, well, volume):
result = self.protocol._context_handler.get_volume(well)
self.assertEqual(volume, result)
def test_transfer(self):
""" Maintain well volumes during transfers. """
self.protocol.add_container('A1', 'microplate.96')
self.protocol.add_container('C1', 'tiprack.p200')
self.protocol.add_instrument('A', 'p200')
self.protocol.calibrate('A1', x=1, y=2, z=3)
self.assertVolume('A1:A2', 0)
self.protocol.transfer('A1:A1', 'A1:A2', ul=100)
self.assertVolume('A1:A2', 100)
self.protocol.transfer('A1:A2', 'A1:A3', ul=20)
self.assertVolume('A1:A3', 20)
self.assertVolume('A1:A2', 80)
run = self.protocol.run()
next(run) # Yield to set progress.
self.assertVolume('A1:A2', 0)
next(run) # transfer('A1:A1', 'A1:A2', ul=100)
self.assertVolume('A1:A2', 100)
next(run) # transfer('A1:A2', 'A1:A3', ul=20)
self.assertVolume('A1:A3', 20)
self.assertVolume('A1:A2', 80)
def test_distribute(self):
self.protocol.add_container('A1', 'microplate.96')
self.protocol.add_container('C1', 'tiprack.p200')
self.protocol.add_instrument('A', 'p200')
self.protocol.distribute('A1:A1', ('A1:B1', {
'ul': 50
}), ('A1:C1', {
'ul': 30
}), ('A1:D1', {
'ul': 40
}))
# Final volumes.
self.assertVolume('A1:A1', -120)
self.assertVolume('A1:B1', 50)
self.assertVolume('A1:C1', 30)
self.assertVolume('A1:D1', 40)
# Try during a run.
run = self.protocol.run()
next(run) # Yield to set progress.
self.assertVolume('A1:A2', 0)
next(run) # Our command.
# Final volumes
self.assertVolume('A1:A1', -120)
self.assertVolume('A1:B1', 50)
self.assertVolume('A1:C1', 30)
self.assertVolume('A1:D1', 40)
def test_consolidate(self):
self.protocol.add_container('A1', 'microplate.96')
self.protocol.add_instrument('A', 'p200')
self.protocol.consolidate('A1:A1', ('A1:B1', {
'ul': 50
}), ('A1:C1', {
'ul': 30
}), ('A1:D1', {
'ul': 40
}))
self.assertVolume('A1:A1', 120)
self.assertVolume('A1:B1', -50)
self.assertVolume('A1:C1', -30)
self.assertVolume('A1:D1', -40)
def test_transfer_group(self):
self.protocol.add_container('A1', 'microplate.96')
self.protocol.add_container('C1', 'tiprack.p200')
self.protocol.add_instrument('A', 'p200')
self.protocol.transfer_group(('A1:A1', 'A1:B1', {
'ul': 50
}), ('A1:A1', 'A1:C1', {
'ul': 50
}), ('A1:A1', 'A1:D1', {
'ul': 30
}),
tool='p200')
self.assertVolume('A1:A1', -130)
self.assertVolume('A1:B1', 50)
self.assertVolume('A1:C1', 50)
self.assertVolume('A1:D1', 30)
def test_find_instrument_by_volume(self):
""" Find instrument by volume. """
self.protocol.add_instrument('A', 'p10')
i = self.protocol._context_handler.get_instrument(has_volume=6)
self.assertEqual(i.supports_volume(6), True)
j = self.protocol._context_handler.get_instrument(has_volume=50)
self.assertEqual(j, None)
self.protocol.add_instrument('B', 'p200')
k = self.protocol._context_handler.get_instrument(has_volume=50)
self.assertEqual(k.name, 'p200')
def test_tip_coordinates(self):
""" Return tip coordinates. """
context = self.protocol._context_handler
self.protocol.add_instrument('A', 'p200')
self.protocol.add_instrument('B', 'p10')
self.protocol.add_container('B1', 'tiprack.p200')
self.protocol.calibrate('B1', axis="A", x=100, y=150, top=60)
self.protocol.add_container('A1', 'tiprack.p10')
self.protocol.calibrate('A1', axis="B", x=200, y=250, top=160)
p200 = context.get_instrument(axis='A')
p10 = context.get_instrument(axis='B')
c1 = context.get_next_tip_coordinates(p200)
self.assertEqual(c1, {'x': 100, 'y': 150, 'top': 60, 'bottom': 0})
c2 = context.get_next_tip_coordinates(p200) # Next tip.
self.assertEqual(c2, {'x': 100, 'y': 159, 'top': 60, 'bottom': 0})
c3 = context.get_next_tip_coordinates(p10)
self.assertEqual(c3, {'x': 200, 'y': 250, 'top': 160, 'bottom': 0})
c4 = context.get_next_tip_coordinates(p10) # Next tip.
self.assertEqual(c4, {'x': 200, 'y': 259, 'top': 160, 'bottom': 0})
def test_tiprack_switch(self):
""" Return second tiprack when first is used up. """
context = self.protocol._context_handler
self.protocol.add_instrument('A', 'p200')
self.protocol.add_container('B1', 'tiprack.p200')
self.protocol.calibrate('B1', axis="A", x=100, y=150, top=60)
self.protocol.add_container('A1', 'tiprack.p200')
self.protocol.calibrate('A1', axis="A", x=200, y=250, top=160)
p200 = context.get_instrument(axis='A')
rack = context.find_container(name='tiprack.p200', has_tips=True)
self.assertEqual([(0, 0)], rack.address)
rack.set_tips_used(95) # We've used all but one tip from this rack.
c1 = context.get_next_tip_coordinates(p200) # Last tip.
h12 = [(0, 0), (7, 11)]
self.assertEqual(c1, context.get_coordinates(h12, axis="A"))
rack = context.find_container(name='tiprack.p200', has_tips=True)
self.assertEqual([(1, 0)], rack.address)
def test_multichannel_search(self):
""" Find a multichannel pipette. """
context = self.protocol._context_handler
self.protocol.add_instrument('A', 'p200')
self.protocol.add_instrument('B', 'p20.12')
i1 = context.get_instrument(axis='B')
i2 = context.get_instrument(has_volume=20, channels=12)
self.assertEqual(i1, i2)
i3 = context.get_instrument(has_volume=200, channels=12)
self.assertEqual(i3, None)
def test_multichannel_tip_allocation(self):
context = self.protocol._context_handler
self.protocol.add_instrument('A', 'p20.12')
self.protocol.add_instrument('B', 'p20.8')
self.protocol.add_container('A1', 'tiprack.p20')
a = context.get_instrument(axis='A')
b = context.get_instrument(axis='B')
self.protocol.calibrate('A1', axis="A")
self.protocol.calibrate('A1', axis="B")
# Get a row first.
context.get_next_tip_coordinates(a)
with self.assertRaises(x.TipMissing):
context.get_next_tip_coordinates(b)
# Get a col first.
context = self.protocol.initialize_context()
context.get_next_tip_coordinates(b)
with self.assertRaises(x.TipMissing):
context.get_next_tip_coordinates(a)
# Add another tiprack, get both!
self.protocol.add_container('A2', 'tiprack.p20')
self.protocol.calibrate('A2', axis="A")
self.protocol.calibrate('A2', axis="B")
context = self.protocol.initialize_context()
context.get_next_tip_coordinates(a)
context.get_next_tip_coordinates(b)
# Exhaust the supply.
context = self.protocol.initialize_context()
for _ in range(8):
context.get_next_tip_coordinates(a)
for _ in range(12):
context.get_next_tip_coordinates(b)
with self.assertRaises(x.TipMissing):
context.get_next_tip_coordinates(a)
with self.assertRaises(x.TipMissing):
context.get_next_tip_coordinates(b)
def test_multichannel_transfer_cols(self):
""" Test multichannel transfer (cols). """
self.protocol.add_instrument('A', 'p20.12')
self.protocol.add_instrument('B', 'p20.8')
self.protocol.add_container('A1', 'microplate')
p = self.protocol._context_handler.find_container(name="microplate")
self.protocol.transfer('A1:A1', 'A1:B1', ul=10, tool='p20.12')
self.assertEqual(p.col('A').get_volume(), [-10 for n in range(12)])
self.assertEqual(p.col('B').get_volume(), [10 for n in range(12)])
def test_multichannel_transfer_rows(self):
""" Test multichannel transfer (rows). """
self.protocol.add_instrument('A', 'p20.12')
self.protocol.add_instrument('B', 'p20.8')
self.protocol.add_container('A1', 'microplate')
p = self.protocol._context_handler.find_container(name="microplate")
self.protocol.transfer('A1:A1', 'A1:A2', ul=15, tool='p20.8')
self.assertEqual(p.row(0).get_volume(), [-15 for n in range(8)])
self.assertEqual(p.row(1).get_volume(), [15 for n in range(8)])
class MotorHandlerTest(unittest.TestCase):
def setUp(self):
self.protocol = Protocol()
def test_basic_transfer(self):
""" Basic transfer. """
motor = self.protocol.attach_motor()
output_log = motor._driver
self.protocol.add_instrument('B', 'p200')
self.protocol.add_container('A1', 'microplate.96')
self.protocol.add_container('C1', 'tiprack.p200')
self.protocol.add_container('B2', 'point.trash')
self.protocol.calibrate('A1', x=1, y=2, top=3, bottom=13)
self.protocol.calibrate('A1:A2', bottom=5)
self.protocol.calibrate('C1', x=100, y=100, top=50)
self.protocol.calibrate('B2', x=200, y=250, top=15)
self.protocol.calibrate_instrument('B', top=0, blowout=10, droptip=25)
self.protocol.transfer('A1:A1', 'A1:A2', ul=100)
self.protocol.transfer('A1:A2', 'A1:A3', ul=80)
prog_out = []
for progress in self.protocol.run():
prog_out.append(progress)
expected = [
# Transfer 1.
{'x': 100, 'y': 100}, # Pickup tip.
{'z': 50},
{'z': 0}, # Move to well.
{'x': 1, 'y': 2},
{'z': 3},
{'b': 5.0}, # Plunge.
{'x': 1, 'y': 2},
{'z': 13}, # Move into well.
{'b': 0}, # Release.
{'z': 0}, # Move up.
{'x': 1, 'y': 11}, # Move to well.
{'z': 3},
{'x': 1, 'y': 11},
{'z': 5}, # Move into well.
{'b': 10}, # Blowout.
{'z': 0}, # Move up.
{'b': 0}, # Release.
{'x': 200, 'y': 250}, # Dispose tip.
{'z': 15},
{'b': 25},
{'b': 0},
# Transfer 2.
{'x': 100, 'y': 109},
{'z': 50},
{'z': 0},
{'x': 1, 'y': 11},
{'z': 3},
{'b': 4.0},
{'x': 1, 'y': 11},
{'z': 5},
{'b': 0},
{'z': 0},
{'x': 1, 'y': 20},
{'z': 3},
{'x': 1, 'y': 20},
{'z': 13},
{'b': 10},
{'z': 0},
{'b': 0},
{'x': 200, 'y': 250},
{'z': 15},
{'b': 25},
{'b': 0}
]
self.assertEqual(expected, output_log.movements)
self.assertEqual([(0, 2), (1, 2), (2, 2)], prog_out)
def test_transfer_without_tiprack(self):
""" Raise error when no tiprack found. """
self.protocol.attach_motor()
self.protocol.add_instrument('B', 'p200')
self.protocol.add_container('A1', 'microplate.96')
self.protocol.calibrate_instrument('B', top=0, blowout=10)
self.protocol.transfer('A1:A1', 'A1:A2', ul=100)
self.protocol.transfer('A1:A2', 'A1:A3', ul=80)
with self.assertRaises(KeyError):
for progress in self.protocol.run():
continue
def test_transfer_without_dispose_point(self):
""" Raise when no dispose point set. """
self.protocol.attach_motor()
self.protocol.add_instrument('B', 'p200')
self.protocol.add_container('A1', 'microplate.96')
self.protocol.add_container('C1', 'tiprack.p200')
self.protocol.calibrate_instrument('B', top=0, blowout=10)
self.protocol.transfer('A1:A1', 'A1:A2', ul=100)
self.protocol.transfer('A1:A2', 'A1:A3', ul=80)
with self.assertRaises(KeyError):
for progress in self.protocol.run():
continue