def OnLeftUp(self, event):
'''When left mouse button is lifted up, determine the DNA selection from angles generated at down an up events.'''
amino_acid = self.HitTest()
if amino_acid is not False:
amino_acid = amino_acid.replace('2','')
if amino_acid == 'reset': #special case to catch reset button
self.codon = False
self.target = []
self.offtarget = []
self.possible = []
self.text_edit_active = False #exit text editing mode
elif amino_acid == 'text': #special case to catch the codon edit region
self.text_edit_active = True #switch to text editing mode
elif amino_acid not in self.target:
self.target.append(amino_acid)
self.text_edit_active = False #exit text editing mode
elif amino_acid in self.target:
self.target.remove(amino_acid)
self.text_edit_active = False #exit text editing mode
else:
self.text_edit_active = False #exit text editing mode
if len(self.target)>0:
self.codon, self.target, self.offtarget, self.possible = mbc.run(self.target)
else:
self.codon = False
self.offtarget = []
self.possible = []
self.update_ownUI()
def OnLeftUp(self, event):
'''When left mouse button is lifted up, determine the DNA selection from angles generated at down an up events.'''
amino_acid = self.HitTest()
if amino_acid is not False:
amino_acid = amino_acid.replace('2','')
if amino_acid not in self.target:
self.target.append(amino_acid)
elif amino_acid in self.target:
self.target.remove(amino_acid)
elif amino_acid == 'reset':
self.codon = False
self.target = []
self.offtarget = []
self.possible = []
if len(self.target)>0:
self.codon, self.target, self.offtarget, self.possible = mbc.run(self.target)
else:
self.codon = False
self.offtarget = []
self.possible = []
self.update_ownUI()
def OnToggle(self, evt):
'''When any togglebutton is pressed'''
AA = []
clicked_color = '#A2CD5A'
unclicked_color = '#EEEEEE'
if self.Ala.GetValue():
AA.append('A')
self.Ala.SetBackgroundColour(clicked_color)
else:
self.Ala.SetBackgroundColour(unclicked_color)
if self.Val.GetValue():
AA.append('V')
self.Val.SetBackgroundColour(clicked_color)
else:
self.Val.SetBackgroundColour(unclicked_color)
if self.Ile.GetValue():
AA.append('I')
self.Ile.SetBackgroundColour(clicked_color)
else:
self.Ile.SetBackgroundColour(unclicked_color)
if self.Leu.GetValue():
AA.append('L')
self.Leu.SetBackgroundColour(clicked_color)
else:
self.Leu.SetBackgroundColour(unclicked_color)
if self.Met.GetValue():
AA.append('M')
self.Met.SetBackgroundColour(clicked_color)
else:
self.Met.SetBackgroundColour(unclicked_color)
if self.Phe.GetValue():
AA.append('F')
self.Phe.SetBackgroundColour(clicked_color)
else:
self.Phe.SetBackgroundColour(unclicked_color)
if self.Tyr.GetValue():
AA.append('Y')
self.Tyr.SetBackgroundColour(clicked_color)
else:
self.Tyr.SetBackgroundColour(unclicked_color)
if self.Trp.GetValue():
AA.append('W')
self.Trp.SetBackgroundColour(clicked_color)
else:
self.Trp.SetBackgroundColour(unclicked_color)
if self.Asp.GetValue():
AA.append('D')
self.Asp.SetBackgroundColour(clicked_color)
else:
self.Asp.SetBackgroundColour(unclicked_color)
if self.Glu.GetValue():
AA.append('E')
self.Glu.SetBackgroundColour(clicked_color)
else:
self.Glu.SetBackgroundColour(unclicked_color)
if self.Asn.GetValue():
AA.append('N')
self.Asn.SetBackgroundColour(clicked_color)
else:
self.Asn.SetBackgroundColour(unclicked_color)
if self.Gln.GetValue():
AA.append('Q')
self.Gln.SetBackgroundColour(clicked_color)
else:
self.Gln.SetBackgroundColour(unclicked_color)
if self.Ser.GetValue():
AA.append('S')
self.Ser.SetBackgroundColour(clicked_color)
else:
self.Ser.SetBackgroundColour(unclicked_color)
if self.Thr.GetValue():
AA.append('T')
self.Thr.SetBackgroundColour(clicked_color)
else:
self.Thr.SetBackgroundColour(unclicked_color)
if self.Arg.GetValue():
AA.append('R')
self.Arg.SetBackgroundColour(clicked_color)
else:
self.Arg.SetBackgroundColour(unclicked_color)
if self.His.GetValue():
AA.append('H')
self.His.SetBackgroundColour(clicked_color)
else:
self.His.SetBackgroundColour(unclicked_color)
if self.Lys.GetValue():
AA.append('K')
self.Lys.SetBackgroundColour(clicked_color)
else:
self.Lys.SetBackgroundColour(unclicked_color)
if self.Cys.GetValue():
AA.append('C')
self.Cys.SetBackgroundColour(clicked_color)
else:
self.Cys.SetBackgroundColour(unclicked_color)
if self.Gly.GetValue():
AA.append('G')
self.Gly.SetBackgroundColour(clicked_color)
else:
self.Gly.SetBackgroundColour(unclicked_color)
if self.Pro.GetValue():
AA.append('P')
self.Pro.SetBackgroundColour(clicked_color)
else:
self.Pro.SetBackgroundColour(unclicked_color)
if len(AA) > 0:
codon, target, offtarget, possibleAA = mbc.run(AA)
self.mixed_base_codon.SetReadOnly(False)
self.mixed_base_codon.SetText(codon)
self.mixed_base_codon.SetReadOnly(True)
targetstring = ''
for entry in target: #make string with the target AA
if targetstring == '':
targetstring += entry
else:
targetstring += ', '+entry
offtargetstring = ''
for entry in offtarget: #make string with the offtarget AA
if offtargetstring == '':
offtargetstring += entry
else:
offtargetstring += ', '+entry
self.target_hits.SetLabel('Target amino acids: '+targetstring)
self.offtarget_hits.SetLabel('Off-target amino acids: '+offtargetstring)
#set the yellow buttons to indicate which AA are possible without further off-target hits
possible_color = '#FFFF99'
if 'A' in possibleAA and self.Ala.GetValue() == False:
self.Ala.SetBackgroundColour(possible_color)
if 'V' in possibleAA and self.Val.GetValue() == False:
self.Val.SetBackgroundColour(possible_color)
if 'I' in possibleAA and self.Ile.GetValue() == False:
self.Ile.SetBackgroundColour(possible_color)
if 'L' in possibleAA and self.Leu.GetValue() == False:
self.Leu.SetBackgroundColour(possible_color)
if 'M' in possibleAA and self.Met.GetValue() == False:
self.Met.SetBackgroundColour(possible_color)
if 'F' in possibleAA and self.Phe.GetValue() == False:
self.Phe.SetBackgroundColour(possible_color)
if 'Y' in possibleAA and self.Tyr.GetValue() == False:
self.Tyr.SetBackgroundColour(possible_color)
if 'W' in possibleAA and self.Trp.GetValue() == False:
self.Trp.SetBackgroundColour(possible_color)
if 'D' in possibleAA and self.Asp.GetValue() == False:
self.Asp.SetBackgroundColour(possible_color)
if 'E' in possibleAA and self.Glu.GetValue() == False:
self.Glu.SetBackgroundColour(possible_color)
if 'N' in possibleAA and self.Asn.GetValue() == False:
self.Asn.SetBackgroundColour(possible_color)
if 'Q' in possibleAA and self.Gln.GetValue() == False:
self.Gln.SetBackgroundColour(possible_color)
if 'S' in possibleAA and self.Ser.GetValue() == False:
self.Ser.SetBackgroundColour(possible_color)
if 'T' in possibleAA and self.Thr.GetValue() == False:
self.Thr.SetBackgroundColour(possible_color)
if 'R' in possibleAA and self.Arg.GetValue() == False:
self.Arg.SetBackgroundColour(possible_color)
if 'H' in possibleAA and self.His.GetValue() == False:
self.His.SetBackgroundColour(possible_color)
if 'K' in possibleAA and self.Lys.GetValue() == False:
self.Lys.SetBackgroundColour(possible_color)
if 'C' in possibleAA and self.Cys.GetValue() == False:
self.Cys.SetBackgroundColour(possible_color)
if 'G' in possibleAA and self.Gly.GetValue() == False:
self.Gly.SetBackgroundColour(possible_color)
if 'P' in possibleAA and self.Pro.GetValue() == False:
self.Pro.SetBackgroundColour(possible_color)
#make list of all codons in degenerate triplet
codonlist = dna.UnAmb(codon)
#count how many times each AA is coded for by these codons
self.AA_count = mbc.count_codon_list(codonlist)
#plot graph
self.update_plot()
else:
self.mixed_base_codon.SetReadOnly(False)
self.mixed_base_codon.SetText('None')
self.mixed_base_codon.SetReadOnly(True)
self.target_hits.SetLabel('Target amino acids: ')
self.offtarget_hits.SetLabel('Off-target amino acids: ')
self.AA_count = {'A':0, 'C':0, 'E':0, 'D':0, 'G':0, 'F':0, 'I':0, 'H':0, 'K':0, 'M':0, 'L':0, 'N':0, 'Q':0, 'P':0, 'S':0, 'R':0, 'T':0, 'W':0, 'V':0, 'Y':0, 'stop':0}
self.update_plot()
def OnToggle(self, evt):
'''When any togglebutton is pressed'''
AA = []
clicked_color = '#A2CD5A'
unclicked_color = '#EEEEEE'
if self.Ala.GetValue():
AA.append('A')
self.Ala.SetBackgroundColour(clicked_color)
else:
self.Ala.SetBackgroundColour(unclicked_color)
if self.Val.GetValue():
AA.append('V')
self.Val.SetBackgroundColour(clicked_color)
else:
self.Val.SetBackgroundColour(unclicked_color)
if self.Ile.GetValue():
AA.append('I')
self.Ile.SetBackgroundColour(clicked_color)
else:
self.Ile.SetBackgroundColour(unclicked_color)
if self.Leu.GetValue():
AA.append('L')
self.Leu.SetBackgroundColour(clicked_color)
else:
self.Leu.SetBackgroundColour(unclicked_color)
if self.Met.GetValue():
AA.append('M')
self.Met.SetBackgroundColour(clicked_color)
else:
self.Met.SetBackgroundColour(unclicked_color)
if self.Phe.GetValue():
AA.append('F')
self.Phe.SetBackgroundColour(clicked_color)
else:
self.Phe.SetBackgroundColour(unclicked_color)
if self.Tyr.GetValue():
AA.append('Y')
self.Tyr.SetBackgroundColour(clicked_color)
else:
self.Tyr.SetBackgroundColour(unclicked_color)
if self.Trp.GetValue():
AA.append('W')
self.Trp.SetBackgroundColour(clicked_color)
else:
self.Trp.SetBackgroundColour(unclicked_color)
if self.Asp.GetValue():
AA.append('D')
self.Asp.SetBackgroundColour(clicked_color)
else:
self.Asp.SetBackgroundColour(unclicked_color)
if self.Glu.GetValue():
AA.append('E')
self.Glu.SetBackgroundColour(clicked_color)
else:
self.Glu.SetBackgroundColour(unclicked_color)
if self.Asn.GetValue():
AA.append('N')
self.Asn.SetBackgroundColour(clicked_color)
else:
self.Asn.SetBackgroundColour(unclicked_color)
if self.Gln.GetValue():
AA.append('Q')
self.Gln.SetBackgroundColour(clicked_color)
else:
self.Gln.SetBackgroundColour(unclicked_color)
if self.Ser.GetValue():
AA.append('S')
self.Ser.SetBackgroundColour(clicked_color)
else:
self.Ser.SetBackgroundColour(unclicked_color)
if self.Thr.GetValue():
AA.append('T')
self.Thr.SetBackgroundColour(clicked_color)
else:
self.Thr.SetBackgroundColour(unclicked_color)
if self.Arg.GetValue():
AA.append('R')
self.Arg.SetBackgroundColour(clicked_color)
else:
self.Arg.SetBackgroundColour(unclicked_color)
if self.His.GetValue():
AA.append('H')
self.His.SetBackgroundColour(clicked_color)
else:
self.His.SetBackgroundColour(unclicked_color)
if self.Lys.GetValue():
AA.append('K')
self.Lys.SetBackgroundColour(clicked_color)
else:
self.Lys.SetBackgroundColour(unclicked_color)
if self.Cys.GetValue():
AA.append('C')
self.Cys.SetBackgroundColour(clicked_color)
else:
self.Cys.SetBackgroundColour(unclicked_color)
if self.Gly.GetValue():
AA.append('G')
self.Gly.SetBackgroundColour(clicked_color)
else:
self.Gly.SetBackgroundColour(unclicked_color)
if self.Pro.GetValue():
AA.append('P')
self.Pro.SetBackgroundColour(clicked_color)
else:
self.Pro.SetBackgroundColour(unclicked_color)
if len(AA) > 0:
codon, target, offtarget, possibleAA = mbc.run(AA)
self.mixed_base_codon.SetReadOnly(False)
self.mixed_base_codon.SetText(codon)
self.mixed_base_codon.SetReadOnly(True)
targetstring = ''
for entry in target: #make string with the target AA
if targetstring == '':
targetstring += entry
else:
targetstring += ', ' + entry
offtargetstring = ''
for entry in offtarget: #make string with the offtarget AA
if offtargetstring == '':
offtargetstring += entry
else:
offtargetstring += ', ' + entry
self.target_hits.SetLabel('Target amino acids: ' + targetstring)
self.offtarget_hits.SetLabel('Off-target amino acids: ' +
offtargetstring)
#set the yellow buttons to indicate which AA are possible without further off-target hits
possible_color = '#FFFF99'
if 'A' in possibleAA and self.Ala.GetValue() == False:
self.Ala.SetBackgroundColour(possible_color)
if 'V' in possibleAA and self.Val.GetValue() == False:
self.Val.SetBackgroundColour(possible_color)
if 'I' in possibleAA and self.Ile.GetValue() == False:
self.Ile.SetBackgroundColour(possible_color)
if 'L' in possibleAA and self.Leu.GetValue() == False:
self.Leu.SetBackgroundColour(possible_color)
if 'M' in possibleAA and self.Met.GetValue() == False:
self.Met.SetBackgroundColour(possible_color)
if 'F' in possibleAA and self.Phe.GetValue() == False:
self.Phe.SetBackgroundColour(possible_color)
if 'Y' in possibleAA and self.Tyr.GetValue() == False:
self.Tyr.SetBackgroundColour(possible_color)
if 'W' in possibleAA and self.Trp.GetValue() == False:
self.Trp.SetBackgroundColour(possible_color)
if 'D' in possibleAA and self.Asp.GetValue() == False:
self.Asp.SetBackgroundColour(possible_color)
if 'E' in possibleAA and self.Glu.GetValue() == False:
self.Glu.SetBackgroundColour(possible_color)
if 'N' in possibleAA and self.Asn.GetValue() == False:
self.Asn.SetBackgroundColour(possible_color)
if 'Q' in possibleAA and self.Gln.GetValue() == False:
self.Gln.SetBackgroundColour(possible_color)
if 'S' in possibleAA and self.Ser.GetValue() == False:
self.Ser.SetBackgroundColour(possible_color)
if 'T' in possibleAA and self.Thr.GetValue() == False:
self.Thr.SetBackgroundColour(possible_color)
if 'R' in possibleAA and self.Arg.GetValue() == False:
self.Arg.SetBackgroundColour(possible_color)
if 'H' in possibleAA and self.His.GetValue() == False:
self.His.SetBackgroundColour(possible_color)
if 'K' in possibleAA and self.Lys.GetValue() == False:
self.Lys.SetBackgroundColour(possible_color)
if 'C' in possibleAA and self.Cys.GetValue() == False:
self.Cys.SetBackgroundColour(possible_color)
if 'G' in possibleAA and self.Gly.GetValue() == False:
self.Gly.SetBackgroundColour(possible_color)
if 'P' in possibleAA and self.Pro.GetValue() == False:
self.Pro.SetBackgroundColour(possible_color)
#make list of all codons in degenerate triplet
codonlist = dna.UnAmb(codon)
#count how many times each AA is coded for by these codons
self.AA_count = mbc.count_codon_list(codonlist)
#plot graph
self.update_plot()
else:
self.mixed_base_codon.SetReadOnly(False)
self.mixed_base_codon.SetText('None')
self.mixed_base_codon.SetReadOnly(True)
self.target_hits.SetLabel('Target amino acids: ')
self.offtarget_hits.SetLabel('Off-target amino acids: ')
self.AA_count = {
'A': 0,
'C': 0,
'E': 0,
'D': 0,
'G': 0,
'F': 0,
'I': 0,
'H': 0,
'K': 0,
'M': 0,
'L': 0,
'N': 0,
'Q': 0,
'P': 0,
'S': 0,
'R': 0,
'T': 0,
'W': 0,
'V': 0,
'Y': 0,
'stop': 0
}
self.update_plot()