def anat_qc_workflow(dataset, settings, mod='T1w', name='anatMRIQC'):
"""
One-subject-one-session-one-run pipeline to extract the NR-IQMs from
anatomical images
.. workflow::
import os.path as op
from mriqc.workflows.anatomical import anat_qc_workflow
datadir = op.abspath('data')
wf = anat_qc_workflow([op.join(datadir, 'sub-001/anat/sub-001_T1w.nii.gz')],
settings={'bids_dir': datadir,
'output_dir': op.abspath('out'),
'ants_nthreads': 1,
'no_sub': True})
"""
workflow = pe.Workflow(name=name + mod)
WFLOGGER.info(
'Building anatomical MRI QC workflow, datasets list: %s',
sorted([d.replace(settings['bids_dir'] + '/', '') for d in dataset]))
# Define workflow, inputs and outputs
# 0. Get data
inputnode = pe.Node(niu.IdentityInterface(fields=['in_file']),
name='inputnode')
inputnode.iterables = [('in_file', dataset)]
outputnode = pe.Node(niu.IdentityInterface(fields=['out_json']),
name='outputnode')
# 1. Reorient anatomical image
to_ras = pe.Node(ConformImage(check_dtype=False), name='conform')
# 2. Skull-stripping (afni)
asw = skullstrip_wf(n4_nthreads=settings.get('ants_nthreads', 1),
unifize=False)
# 3. Head mask
hmsk = headmsk_wf()
# 4. Spatial Normalization, using ANTs
norm = spatial_normalization(settings)
# 5. Air mask (with and without artifacts)
amw = airmsk_wf()
# 6. Brain tissue segmentation
segment = pe.Node(fsl.FAST(segments=True,
out_basename='segment',
img_type=int(mod[1])),
name='segmentation',
estimated_memory_gb=3)
# 7. Compute IQMs
iqmswf = compute_iqms(settings, modality=mod)
# Reports
repwf = individual_reports(settings)
# Connect all nodes
workflow.connect([
(inputnode, to_ras, [('in_file', 'in_file')]),
(inputnode, iqmswf, [('in_file', 'inputnode.in_file')]),
(to_ras, asw, [('out_file', 'inputnode.in_file')]),
(asw, segment, [('outputnode.out_file', 'in_files')]),
(asw, hmsk, [('outputnode.bias_corrected', 'inputnode.in_file')]),
(segment, hmsk, [('tissue_class_map', 'inputnode.in_segm')]),
(asw, norm, [('outputnode.bias_corrected', 'inputnode.moving_image'),
('outputnode.out_mask', 'inputnode.moving_mask')]),
(norm, amw, [('outputnode.inverse_composite_transform',
'inputnode.inverse_composite_transform')]),
(norm, iqmswf, [('outputnode.inverse_composite_transform',
'inputnode.inverse_composite_transform')]),
(norm, repwf, ([('outputnode.out_report', 'inputnode.mni_report')])),
(to_ras, amw, [('out_file', 'inputnode.in_file')]),
(asw, amw, [('outputnode.out_mask', 'inputnode.in_mask')]),
(hmsk, amw, [('outputnode.out_file', 'inputnode.head_mask')]),
(to_ras, iqmswf, [('out_file', 'inputnode.in_ras')]),
(asw, iqmswf, [('outputnode.bias_corrected',
'inputnode.inu_corrected'),
('outputnode.bias_image', 'inputnode.in_inu'),
('outputnode.out_mask', 'inputnode.brainmask')]),
(amw, iqmswf, [('outputnode.out_file', 'inputnode.airmask'),
('outputnode.artifact_msk', 'inputnode.artmask'),
('outputnode.rot_mask', 'inputnode.rotmask')]),
(segment, iqmswf, [('tissue_class_map', 'inputnode.segmentation'),
('partial_volume_files', 'inputnode.pvms')]),
(hmsk, iqmswf, [('outputnode.out_file', 'inputnode.headmask')]),
(to_ras, repwf, [('out_file', 'inputnode.in_ras')]),
(asw, repwf, [('outputnode.bias_corrected', 'inputnode.inu_corrected'),
('outputnode.out_mask', 'inputnode.brainmask')]),
(hmsk, repwf, [('outputnode.out_file', 'inputnode.headmask')]),
(amw, repwf, [('outputnode.out_file', 'inputnode.airmask'),
('outputnode.artifact_msk', 'inputnode.artmask'),
('outputnode.rot_mask', 'inputnode.rotmask')]),
(segment, repwf, [('tissue_class_map', 'inputnode.segmentation')]),
(iqmswf, repwf, [('outputnode.out_noisefit', 'inputnode.noisefit')]),
(iqmswf, repwf, [('outputnode.out_file', 'inputnode.in_iqms')]),
(iqmswf, outputnode, [('outputnode.out_file', 'out_json')])
])
# Upload metrics
if not settings.get('no_sub', False):
from ..interfaces.webapi import UploadIQMs
upldwf = pe.Node(UploadIQMs(), name='UploadMetrics')
upldwf.inputs.email = settings.get('email', '')
upldwf.inputs.url = settings.get('webapi_url')
if settings.get('webapi_port'):
upldwf.inputs.port = settings.get('webapi_port')
upldwf.inputs.strict = settings.get('upload_strict', False)
workflow.connect([
(iqmswf, upldwf, [('outputnode.out_file', 'in_iqms')]),
])
return workflow
def init_anat_preproc_wf(skull_strip_template, output_spaces, template, debug,
freesurfer, longitudinal, omp_nthreads, hires, reportlets_dir,
output_dir, num_t1w,
name='anat_preproc_wf'):
r"""
This workflow controls the anatomical preprocessing stages of FMRIPREP.
This includes:
- Creation of a structural template
- Skull-stripping and bias correction
- Tissue segmentation
- Normalization
- Surface reconstruction with FreeSurfer
.. workflow::
:graph2use: orig
:simple_form: yes
from fmriprep.workflows.anatomical import init_anat_preproc_wf
wf = init_anat_preproc_wf(omp_nthreads=1,
reportlets_dir='.',
output_dir='.',
template='MNI152NLin2009cAsym',
output_spaces=['T1w', 'fsnative',
'template', 'fsaverage5'],
skull_strip_template='OASIS',
freesurfer=True,
longitudinal=False,
debug=False,
hires=True,
num_t1w=1)
**Parameters**
skull_strip_template : str
Name of ANTs skull-stripping template ('OASIS' or 'NKI')
output_spaces : list
List of output spaces functional images are to be resampled to.
Some pipeline components will only be instantiated for some output spaces.
Valid spaces:
- T1w
- template
- fsnative
- fsaverage (or other pre-existing FreeSurfer templates)
template : str
Name of template targeted by `'template'` output space
debug : bool
Enable debugging outputs
freesurfer : bool
Enable FreeSurfer surface reconstruction (may increase runtime)
longitudinal : bool
Create unbiased structural template, regardless of number of inputs
(may increase runtime)
omp_nthreads : int
Maximum number of threads an individual process may use
hires : bool
Enable sub-millimeter preprocessing in FreeSurfer
reportlets_dir : str
Directory in which to save reportlets
output_dir : str
Directory in which to save derivatives
name : str, optional
Workflow name (default: anat_preproc_wf)
**Inputs**
t1w
List of T1-weighted structural images
t2w
List of T2-weighted structural images
subjects_dir
FreeSurfer SUBJECTS_DIR
**Outputs**
t1_preproc
Bias-corrected structural template, defining T1w space
t1_brain
Skull-stripped ``t1_preproc``
t1_mask
Mask of the skull-stripped template image
t1_seg
Segmentation of preprocessed structural image, including
gray-matter (GM), white-matter (WM) and cerebrospinal fluid (CSF)
t1_tpms
List of tissue probability maps in T1w space
t1_2_mni
T1w template, normalized to MNI space
t1_2_mni_forward_transform
ANTs-compatible affine-and-warp transform file
t1_2_mni_reverse_transform
ANTs-compatible affine-and-warp transform file (inverse)
mni_mask
Mask of skull-stripped template, in MNI space
mni_seg
Segmentation, resampled into MNI space
mni_tpms
List of tissue probability maps in MNI space
subjects_dir
FreeSurfer SUBJECTS_DIR
subject_id
FreeSurfer subject ID
t1_2_fsnative_forward_transform
LTA-style affine matrix translating from T1w to FreeSurfer-conformed subject space
t1_2_fsnative_reverse_transform
LTA-style affine matrix translating from FreeSurfer-conformed subject space to T1w
surfaces
GIFTI surfaces (gray/white boundary, midthickness, pial, inflated)
**Subworkflows**
* :py:func:`~fmriprep.workflows.anatomical.init_skullstrip_ants_wf`
* :py:func:`~fmriprep.workflows.anatomical.init_surface_recon_wf`
"""
workflow = pe.Workflow(name=name)
inputnode = pe.Node(
niu.IdentityInterface(fields=['t1w', 't2w', 'subjects_dir', 'subject_id']),
name='inputnode')
outputnode = pe.Node(niu.IdentityInterface(
fields=['t1_preproc', 't1_brain', 't1_mask', 't1_seg', 't1_tpms',
't1_2_mni', 't1_2_mni_forward_transform', 't1_2_mni_reverse_transform',
'mni_mask', 'mni_seg', 'mni_tpms',
'template_transforms',
'subjects_dir', 'subject_id', 't1_2_fsnative_forward_transform',
't1_2_fsnative_reverse_transform', 'surfaces']),
name='outputnode')
buffernode = pe.Node(niu.IdentityInterface(
fields=['t1_brain', 't1_mask']), name='buffernode')
anat_template_wf = init_anat_template_wf(longitudinal=longitudinal, omp_nthreads=omp_nthreads,
num_t1w=num_t1w)
# 3. Skull-stripping
# Bias field correction is handled in skull strip workflows.
skullstrip_ants_wf = init_skullstrip_ants_wf(name='skullstrip_ants_wf',
skull_strip_template=skull_strip_template,
debug=debug,
omp_nthreads=omp_nthreads)
workflow.connect([
(inputnode, anat_template_wf, [('t1w', 'inputnode.t1w')]),
(anat_template_wf, skullstrip_ants_wf, [('outputnode.t1_template', 'inputnode.in_file')]),
(skullstrip_ants_wf, outputnode, [('outputnode.bias_corrected', 't1_preproc')]),
(anat_template_wf, outputnode, [
('outputnode.template_transforms', 't1_template_transforms')]),
(buffernode, outputnode, [('t1_brain', 't1_brain'),
('t1_mask', 't1_mask')]),
])
# 4. Surface reconstruction
if freesurfer:
surface_recon_wf = init_surface_recon_wf(name='surface_recon_wf',
omp_nthreads=omp_nthreads, hires=hires)
applyrefined = pe.Node(fsl.ApplyMask(), name='applyrefined')
workflow.connect([
(inputnode, surface_recon_wf, [
('t2w', 'inputnode.t2w'),
('subjects_dir', 'inputnode.subjects_dir'),
('subject_id', 'inputnode.subject_id')]),
(anat_template_wf, surface_recon_wf, [('outputnode.t1_template', 'inputnode.t1w')]),
(skullstrip_ants_wf, surface_recon_wf, [
('outputnode.out_file', 'inputnode.skullstripped_t1'),
('outputnode.out_segs', 'inputnode.ants_segs'),
('outputnode.bias_corrected', 'inputnode.corrected_t1')]),
(skullstrip_ants_wf, applyrefined, [
('outputnode.bias_corrected', 'in_file')]),
(surface_recon_wf, applyrefined, [
('outputnode.out_brainmask', 'mask_file')]),
(surface_recon_wf, outputnode, [
('outputnode.subjects_dir', 'subjects_dir'),
('outputnode.subject_id', 'subject_id'),
('outputnode.t1_2_fsnative_forward_transform', 't1_2_fsnative_forward_transform'),
('outputnode.t1_2_fsnative_reverse_transform', 't1_2_fsnative_reverse_transform'),
('outputnode.surfaces', 'surfaces')]),
(applyrefined, buffernode, [('out_file', 't1_brain')]),
(surface_recon_wf, buffernode, [
('outputnode.out_brainmask', 't1_mask')]),
])
else:
workflow.connect([
(skullstrip_ants_wf, buffernode, [
('outputnode.out_file', 't1_brain'),
('outputnode.out_mask', 't1_mask')]),
])
# 5. Segmentation
t1_seg = pe.Node(fsl.FAST(segments=True, no_bias=True, probability_maps=True),
name='t1_seg', mem_gb=3)
workflow.connect([
(buffernode, t1_seg, [('t1_brain', 'in_files')]),
(t1_seg, outputnode, [('tissue_class_map', 't1_seg'),
('probability_maps', 't1_tpms')]),
])
# 6. Spatial normalization (T1w to MNI registration)
t1_2_mni = pe.Node(
RobustMNINormalizationRPT(
float=True,
generate_report=True,
flavor='testing' if debug else 'precise',
),
name='t1_2_mni',
n_procs=omp_nthreads,
mem_gb=2
)
# Resample the brain mask and the tissue probability maps into mni space
mni_mask = pe.Node(
ApplyTransforms(dimension=3, default_value=0, float=True,
interpolation='NearestNeighbor'),
name='mni_mask'
)
mni_seg = pe.Node(
ApplyTransforms(dimension=3, default_value=0, float=True,
interpolation='NearestNeighbor'),
name='mni_seg'
)
mni_tpms = pe.MapNode(
ApplyTransforms(dimension=3, default_value=0, float=True,
interpolation='Linear'),
iterfield=['input_image'],
name='mni_tpms'
)
if 'template' in output_spaces:
template_str = nid.TEMPLATE_MAP[template]
ref_img = op.join(nid.get_dataset(template_str), '1mm_T1.nii.gz')
t1_2_mni.inputs.template = template_str
mni_mask.inputs.reference_image = ref_img
mni_seg.inputs.reference_image = ref_img
mni_tpms.inputs.reference_image = ref_img
workflow.connect([
(skullstrip_ants_wf, t1_2_mni, [('outputnode.bias_corrected', 'moving_image')]),
(buffernode, t1_2_mni, [('t1_mask', 'moving_mask')]),
(buffernode, mni_mask, [('t1_mask', 'input_image')]),
(t1_2_mni, mni_mask, [('composite_transform', 'transforms')]),
(t1_seg, mni_seg, [('tissue_class_map', 'input_image')]),
(t1_2_mni, mni_seg, [('composite_transform', 'transforms')]),
(t1_seg, mni_tpms, [('probability_maps', 'input_image')]),
(t1_2_mni, mni_tpms, [('composite_transform', 'transforms')]),
(t1_2_mni, outputnode, [
('warped_image', 't1_2_mni'),
('composite_transform', 't1_2_mni_forward_transform'),
('inverse_composite_transform', 't1_2_mni_reverse_transform')]),
(mni_mask, outputnode, [('output_image', 'mni_mask')]),
(mni_seg, outputnode, [('output_image', 'mni_seg')]),
(mni_tpms, outputnode, [('output_image', 'mni_tpms')]),
])
seg2msks = pe.Node(niu.Function(function=_seg2msks), name='seg2msks')
seg_rpt = pe.Node(ROIsPlot(colors=['r', 'magenta', 'b', 'g']), name='seg_rpt')
anat_reports_wf = init_anat_reports_wf(
reportlets_dir=reportlets_dir, output_spaces=output_spaces, template=template,
freesurfer=freesurfer)
workflow.connect([
(inputnode, anat_reports_wf, [
(('t1w', fix_multi_T1w_source_name), 'inputnode.source_file')]),
(anat_template_wf, anat_reports_wf, [
('outputnode.out_report', 'inputnode.t1_conform_report')]),
(anat_template_wf, seg_rpt, [
('outputnode.t1_template', 'in_file')]),
(t1_seg, seg2msks, [('tissue_class_map', 'in_file')]),
(seg2msks, seg_rpt, [('out', 'in_rois')]),
(outputnode, seg_rpt, [('t1_mask', 'in_mask')]),
(seg_rpt, anat_reports_wf, [('out_report', 'inputnode.seg_report')]),
])
if freesurfer:
workflow.connect([
(surface_recon_wf, anat_reports_wf, [
('outputnode.out_report', 'inputnode.recon_report')])
])
if 'template' in output_spaces:
workflow.connect([
(t1_2_mni, anat_reports_wf, [('out_report', 'inputnode.t1_2_mni_report')]),
])
anat_derivatives_wf = init_anat_derivatives_wf(output_dir=output_dir,
output_spaces=output_spaces,
template=template,
freesurfer=freesurfer)
workflow.connect([
(anat_template_wf, anat_derivatives_wf, [
('outputnode.t1w_valid_list', 'inputnode.source_files')]),
(outputnode, anat_derivatives_wf, [
('t1_template_transforms', 'inputnode.t1_template_transforms'),
('t1_preproc', 'inputnode.t1_preproc'),
('t1_mask', 'inputnode.t1_mask'),
('t1_seg', 'inputnode.t1_seg'),
('t1_tpms', 'inputnode.t1_tpms'),
('t1_2_mni_forward_transform', 'inputnode.t1_2_mni_forward_transform'),
('t1_2_mni_reverse_transform', 'inputnode.t1_2_mni_reverse_transform'),
('t1_2_mni', 'inputnode.t1_2_mni'),
('mni_mask', 'inputnode.mni_mask'),
('mni_seg', 'inputnode.mni_seg'),
('mni_tpms', 'inputnode.mni_tpms'),
('t1_2_fsnative_forward_transform', 'inputnode.t1_2_fsnative_forward_transform'),
('surfaces', 'inputnode.surfaces'),
]),
])
return workflow