def main(qbed, sbed, pairs_file, qpad, spad, pair_fmt, blast_path, mask='F', ncpu=8):
"""main runner for finding cnss"""
pool = Pool(ncpu)
bl2seq = determine_blast_type(blast_path,mask)
# legacy_bl2seq = "%s " % blast_path + \
# " bl2seq -p blastn -D 1 -E 2 -q -2 -r 1 -G 5 -W 7 -F %s " % mask + \
# " -e %(e_value).2f -i %(qfasta)s -j %(sfasta)s \
# -I %(qstart)d,%(qstop)d -J %(sstart)d,%(sstop)d | grep -v '#' \
# | grep -v 'WARNING' | grep -v 'ERROR' "
#
# bl2seq = legacy_bl2seq
#
# bl2seq = "%s " % blast_path + \
# " -p blastn -D 1 -E 2 -q -2 -r 1 -G 5 -W 7 -F %s " % mask + \
# " -e %(e_value).2f -i %(qfasta)s -j %(sfasta)s \
# -I %(qstart)d,%(qstop)d -J %(sstart)d,%(sstop)d | grep -v '#' \
# | grep -v 'WARNING' | grep -v 'ERROR' "
#
fcnss = sys.stdout
print >> fcnss, "#qseqid,qaccn,sseqid,saccn,[qstart,qend,sstart,send,bitscore...]"
qfastas = get_masked_fastas(qbed)
sfastas = get_masked_fastas(sbed) if qbed.filename != sbed.filename else qfastas
pairs = [True]
_get_pair_gen = get_pair(pairs_file, pair_fmt, qbed, sbed)
# need this for parallization stuff.
def get_pair_gen():
try: return _get_pair_gen.next()
except StopIteration: return None
while any(pairs):
pairs = [get_pair_gen() for i in range(ncpu)]
# this helps in parallization
spad_map = [spad] * len(pairs)
qpad_map = [qpad] * len(pairs)
sfastas_map = [sfastas] * len(pairs)
qfastas_map = [qfastas] * len(pairs)
bl2seq_map = [bl2seq] * len(pairs)
#################################
cmds = [c for c in map(get_cmd, [l for l in pairs if
l],bl2seq_map,qfastas_map,sfastas_map,qpad_map,spad_map) if c]
#print >>sys.stderr ,cmds
results = (r for r in pool.map(commands.getoutput, [c[0] for c in cmds]))
for res, (cmd, qfeat, sfeat) in zip(results, cmds):
if not res.strip(): continue
print >>sys.stderr, "%s %s" % (qfeat["accn"], sfeat['accn']),
orient = qfeat['strand'] == sfeat['strand'] and 1 or -1
cnss = parse_blast(res, orient, qfeat, sfeat, qbed, sbed, qpad, spad)
print >>sys.stderr, "(%i)" % len(cnss)
if len(cnss) == 0: continue
qname, sname = qfeat['accn'], sfeat['accn']
print >> fcnss, "%s,%s,%s,%s,%s" % (qfeat['seqid'], qname, sfeat['seqid'], sname,
",".join(map(lambda l: ",".join(map(str,l)),cnss)))
return None
def main(qbed, sbed, pairs_file, qpad, spad, unmasked_fasta, pair_fmt,blast_path, mask='F', ncpu=8):
"""main runner for finding cnss"""
pool = Pool(ncpu)
bl2seq = "%s " % blast_path + \
"-p blastn -D 1 -E 2 -q -2 -r 1 -G 5 -W 7 -F %s " % mask + \
" -e %(e_value).2f -i %(qfasta)s -j %(sfasta)s \
-I %(qstart)d,%(qstop)d -J %(sstart)d,%(sstop)d | grep -v '#' \
| grep -v 'WARNING' | grep -v 'ERROR' "
fcnss = sys.stdout
print >> fcnss,
"#qseqid,qaccn,sseqid,saccn,[qstart,qend,sstart,send,bitscore...]"
qfastas = get_masked_fastas(qbed)
sfastas = get_masked_fastas(sbed) if qbed.filename != sbed.filename else qfastas
pairs = [True]
_get_pair_gen = get_pair(pairs_file, pair_fmt, qbed, sbed)
# need this for parallization stuff.
def get_pair_gen():
try: return _get_pair_gen.next()
except StopIteration: return None
while any(pairs):
pairs = [get_pair_gen() for i in range(ncpu)]
# this helps in parallelizing.
spad_map = [spad] * len(pairs)
qpad_map = [qpad] * len(pairs)
sfastas_map = [sfastas] * len(pairs)
qfastas_map = [qfastas] * len(pairs)
bl2seq_map = [bl2seq] * len(pairs)
####################################
cmds = [c for c in map(get_cmd, [l for l in pairs if
l],bl2seq_map,qfastas_map,sfastas_map,qpad_map,spad_map) if c]
results = (r for r in pool.map(commands.getoutput, [c[0] for c in cmds]))
for res, (cmd, qfeat, sfeat) in zip(results, cmds):
if not res.strip(): continue
print >>sys.stderr, "%s %s" % (qfeat["accn"], sfeat['accn']),
orient = qfeat['strand'] == sfeat['strand'] and 1 or -1
cnss = parse_blast(res, orient, qfeat, sfeat, qbed, sbed, qpad, spad, unmasked_fasta)
print >>sys.stderr, "(%i)" % len(cnss)
if len(cnss) == 0: continue
qname, sname = qfeat['accn'], sfeat['accn']
print >> fcnss, "%s,%s,%s,%s,%s" % (qfeat['seqid'], qname, sfeat['seqid'], sname,
",".join(map(lambda l: ",".join(map(str,l)),cnss)))
return None
def main(cns_bed, ortho_bed, pairs_file, qpad, spad, blast_path, ncpu, mask):
"imput a cns_dict and otholog_dict, cns_bed "
pool = Pool(ncpu)
fcns = sys.stdout
print >> fcns, "#cns_start, cns_stop, interval"
bl2seq = "%s " % blast_path + \
"-p blastn -D 1 -E 2 -q -2 -r 1 -G 5 -W 7 -F %s " % mask + \
" -e %(e_value).2f -i %(qfasta)s -j %(sfasta)s \
-I %(qstart)d,%(qstop)d -J %(sstart)d,%(sstop)d | grep -v '#' \
| grep -v 'WARNING' | grep -v 'ERROR' "
qfastas = get_fastas(cns_bed, True)
sfastas = get_fastas(ortho_bed, False)
pairs = [True]
_get_pair_gen = get_pair(pairs_file ,'pck', ortho_bed, cns_bed)
def get_pair_gen():
try: return _get_pair_gen.next()
except StopIteration: return None
while any(pairs):
pairs = [get_pair_gen() for i in range(ncpu)]
def get_cmd(pairs):
qfeat, sfeat = pairs
if pairs is None: return None
sfasta = sfastas[sfeat['seqid']]
qfasta = qfastas[qfeat['seqid']]
qstart, qstop = max(qfeat['start'] - qpad, 1), qfeat['end'] + qpad
sstart, sstop = max(sfeat['start'] - spad, 1), sfeat['end'] + spad
m = qstop - qstart
n = sstop - sstart
e_value = m*n*(2**(-28.51974)) # bit score above 15/15 noise
assert e_value > 0
cmd = bl2seq % dict(qfasta=qfasta, sfasta=sfasta, qstart=qstart,
sstart=sstart, qstop=qstop, sstop=sstop, e_value=e_value)
return cmd, qfeat
cmds = [c for c in map(get_cmd, [l for l in pairs if l]) if c]
results = (r for r in pool.map(commands.getoutput, [c[0] for c in cmds]))
#cmd = get_cmd(qfeat, sfeat, qpad, spad, mask)
for res,(cmd, qfeat) in zip(results,cmds):
if not res.strip(): continue #ask tom?
interval = parse_blast(res, qfeat)
if len(interval) >= 1:
print >> fcns, '{0},{1},{2}'.format(qfeat['start'], qfeat['end'], interval)
def run():
import random
from processing import Pool
p=Pool(2)
print "ahora vemos si escala"
numero = 5000000
a = [random.randint(0, 100) for a in xrange(0, numero)]
print "Ya tenemos los numeros"
lista = [a[:numero/2] , a[numero/2:]]
print "Lista bisectada"
result=p.mapAsync(my_sort, lista)
print "threads lanzados"
lista1, lista2 = result.get()
print "Uniendo listas"
b = my_merge(lista1, lista2)
# b = my_sort(a)
print "largo", len(b), "llamadas a my_sort"
def main(qbed, sbed, pairs_file, qpad, spad, unmasked_fasta, pair_fmt, mask='F', ncpu=8):
"""main runner for finding cnss"""
pool = Pool(options.ncpu)
bl2seq = "~/src/blast-2.2.25/bin/bl2seq " \
"-p blastn -D 1 -E 2 -q -2 -r 1 -G 5 -W 7 -F %s " % mask + \
" -e %(e_value).2f -i %(qfasta)s -j %(sfasta)s \
-I %(qstart)d,%(qstop)d -J %(sstart)d,%(sstop)d | grep -v '#' \
| grep -v 'WARNING' | grep -v 'ERROR' "
fcnss = sys.stdout
print >> fcnss, "#qseqid,qaccn,sseqid,saccn,[qstart,qend,sstart,send...]"
qfastas = get_masked_fastas(qbed)
sfastas = get_masked_fastas(sbed) if qbed.filename != sbed.filename else qfastas
pairs = [True]
_get_pair_gen = get_pair(pairs_file, pair_fmt, qbed, sbed)
# need this for parallization stuff.
def get_pair_gen():
try: return _get_pair_gen.next()
except StopIteration: return None
while any(pairs):
pairs = [get_pair_gen() for i in range(ncpu)]
# this helps in parallelizing.
def get_cmd(pair):
if pair is None: return None
qfeat, sfeat = pair
#if qfeat['accn'] != "Bradi4g01820": return None
#print >>sys.stderr, qfeat, sfeat
qfasta = qfastas[qfeat['seqid']]
sfasta = sfastas[sfeat['seqid']]
qstart, qstop = max(qfeat['start'] - qpad, 1), qfeat['end'] + qpad
sstart, sstop = max(sfeat['start'] - spad, 1), sfeat['end'] + spad
assert qstop - qstart > 2 * qpad or qstart == 1, (qstop, qstart)
assert sstop - sstart > 2 * spad or sstart == 1, (sstop, sstart)
m = qstop - qstart
n = sstop - sstart
# if (m*n) >= 812045000: # if the database and query is large keep e_value at 2.11 else change it to something smaller
# e_value = 2.11
# else:
e_value = m*n*(2**(-28.51974)) # bit score above 15/15 noise
assert e_value > 0
cmd = bl2seq % dict(qfasta=qfasta, sfasta=sfasta, qstart=qstart,
sstart=sstart, qstop=qstop, sstop=sstop, e_value=e_value)
#print >>sys.stderr, "%s" % (cmd)
return cmd, qfeat, sfeat
cmds = [c for c in map(get_cmd, [l for l in pairs if l]) if c]
results = (r for r in pool.map(commands.getoutput, [c[0] for c in cmds]))
#results = (r for r in map(commands.getoutput, [c[0] for c in cmds]))
for res, (cmd, qfeat, sfeat) in zip(results, cmds):
if not res.strip(): continue
print >>sys.stderr, "%s %s" % (qfeat["accn"], sfeat['accn']),
orient = qfeat['strand'] == sfeat['strand'] and 1 or -1
cnss = parse_blast(res, orient, qfeat, sfeat, qbed, sbed, qpad, spad, unmasked_fasta)
print >>sys.stderr, "(%i)" % len(cnss)
if len(cnss) == 0: continue
qname, sname = qfeat['accn'], sfeat['accn']
print >> fcnss, "%s,%s,%s,%s,%s" % (qfeat['seqid'], qname, sfeat['seqid'], sname,
",".join(map(lambda l: ",".join(map(str,l)),cnss)))
return None
def test():
print 'cpuCount() = %d\n' % cpuCount()
#
# Create pool
#
PROCESSES = 4
print 'Creating pool with %d processes\n' % PROCESSES
pool = Pool(PROCESSES)
#
# Tests
#
TASKS = [(mul, (i, 7)) for i in range(10)] + \
[(plus, (i, 8)) for i in range(10)]
results = [pool.applyAsync(calculate, t) for t in TASKS]
imap_it = pool.imap(calculatestar, TASKS)
imap_unordered_it = pool.imapUnordered(calculatestar, TASKS)
print 'Ordered results using pool.applyAsync():'
for r in results:
print '\t', r.get()
print
print 'Ordered results using pool.imap():'
for x in imap_it:
print '\t', x
print
print 'Unordered results using pool.imapUnordered():'
for x in imap_unordered_it:
print '\t', x
print
print 'Ordered results using pool.map() --- will block till complete:'
for x in pool.map(calculatestar, TASKS):
print '\t', x
print
#
# Simple benchmarks
#
N = 100000
print 'def pow3(x): return x**3'
t = time.time()
A = map(pow3, xrange(N))
print '\tmap(pow3, xrange(%d)):\n\t\t%s seconds' % \
(N, time.time() - t)
t = time.time()
B = pool.map(pow3, xrange(N))
print '\tpool.map(pow3, xrange(%d)):\n\t\t%s seconds' % \
(N, time.time() - t)
t = time.time()
C = list(pool.imap(pow3, xrange(N), chunksize=N // 8))
print '\tlist(pool.imap(pow3, xrange(%d), chunksize=%d)):\n\t\t%s' \
' seconds' % (N, N//8, time.time() - t)
assert A == B == C, (len(A), len(B), len(C))
print
L = [None] * 1000000
print 'def noop(x): pass'
print 'L = [None] * 1000000'
t = time.time()
A = map(noop, L)
print '\tmap(noop, L):\n\t\t%s seconds' % \
(time.time() - t)
t = time.time()
B = pool.map(noop, L)
print '\tpool.map(noop, L):\n\t\t%s seconds' % \
(time.time() - t)
t = time.time()
C = list(pool.imap(noop, L, chunksize=len(L) // 8))
print '\tlist(pool.imap(noop, L, chunksize=%d)):\n\t\t%s seconds' % \
(len(L)//8, time.time() - t)
assert A == B == C, (len(A), len(B), len(C))
print
del A, B, C, L
#
# Test error handling
#
print 'Testing error handling:'
try:
print pool.apply(f, (5, ))
except ZeroDivisionError:
print '\tGot ZeroDivisionError as expected from pool.apply()'
else:
raise AssertionError, 'expected ZeroDivisionError'
try:
print pool.map(f, range(10))
except ZeroDivisionError:
print '\tGot ZeroDivisionError as expected from pool.map()'
else:
raise AssertionError, 'expected ZeroDivisionError'
try:
print list(pool.imap(f, range(10)))
except ZeroDivisionError:
print '\tGot ZeroDivisionError as expected from list(pool.imap())'
else:
raise AssertionError, 'expected ZeroDivisionError'
it = pool.imap(f, range(10))
for i in range(10):
try:
x = it.next()
except ZeroDivisionError:
if i == 5:
pass
except StopIteration:
break
else:
if i == 5:
raise AssertionError, 'expected ZeroDivisionError'
assert i == 9
print '\tGot ZeroDivisionError as expected from IMapIterator.next()'
print
#
# Testing timeouts
#
print 'Testing ApplyResult.get() with timeout:',
res = pool.applyAsync(calculate, TASKS[0])
while 1:
sys.stdout.flush()
try:
sys.stdout.write('\n\t%s' % res.get(0.02))
break
except TimeoutError:
sys.stdout.write('.')
print
print
print 'Testing IMapIterator.next() with timeout:',
it = pool.imap(calculatestar, TASKS)
while 1:
sys.stdout.flush()
try:
sys.stdout.write('\n\t%s' % it.next(0.02))
except StopIteration:
break
except TimeoutError:
sys.stdout.write('.')
print
print
#
# Testing callback
#
print 'Testing callback:'
A = []
B = [56, 0, 1, 8, 27, 64, 125, 216, 343, 512, 729]
r = pool.applyAsync(mul, (7, 8), callback=A.append)
r.wait()
r = pool.mapAsync(pow3, range(10), callback=A.extend)
r.wait()
if A == B:
print '\tcallbacks succeeded\n'
else:
print '\t*** callbacks failed\n\t\t%s != %s\n' % (A, B)
#
# Check there are no outstanding tasks
#
assert not pool._cache, 'cache = %r' % pool._cache
#
# Check close() methods
#
print 'Testing close():'
for worker in pool._pool:
assert worker.isAlive()
result = pool.applyAsync(time.sleep, [0.5])
pool.close()
pool.join()
assert result.get() is None
for worker in pool._pool:
assert not worker.isAlive()
print '\tclose() succeeded\n'
#
# Check terminate() method
#
print 'Testing terminate():'
pool = Pool(2)
ignore = pool.apply(pow3, [2])
results = [pool.applyAsync(time.sleep, [10]) for i in range(10)]
pool.terminate()
pool.join()
for worker in pool._pool:
assert not worker.isAlive()
print '\tterminate() succeeded\n'
#
# Check garbage collection
#
print 'Testing garbage collection:'
pool = Pool(2)
processes = pool._pool
ignore = pool.apply(pow3, [2])
results = [pool.applyAsync(time.sleep, [10]) for i in range(10)]
del results, pool
time.sleep(0.2)
for worker in processes:
assert not worker.isAlive()
print '\tgarbage collection succeeded\n'
import os, shutil, subprocess, zipfile, random
from processing import Pool
from zipfile import ZipFile
from config import *
def unzip(params):
base, ext = params
zf = zipfile.ZipFile(rawDir + '/' + base + '.' + ext, 'r')
for name in zf.namelist():
if name != 'PIC/':
print name
zf.extract(name, extractDir)
files = []
for f in os.listdir(rawDir):
if (f.endswith('exe')):
base, ext = f.split('.')
if (len(base) == 3):
files.append((base, ext))
p = Pool()
p.map(unzip, files)
def main(qbed, sbed, pairs_file, pair_fmt, mask='F', ncpu=8):
"""main runner for finding cnss"""
pool = Pool(options.ncpu)
bl2seq = "~/src/blast-2.2.25/bin/bl2seq " \
"-p blastn -D 1 -E 2 -q -2 -r 1 -G 5 -W 7 -F %s " % mask + \
" -e %(e_value).2f -i %(qfasta)s -j %(sfasta)s \
-I %(qstart)d,%(qstop)d -J %(sstart)d,%(sstop)d | grep -v '#' \
| grep -v 'WARNING' | grep -v 'ERROR' "
fcnss = sys.stdout
print >> fcnss, "#qaccn,qseqid,saccn,[sleft_gene,sright_gene],sseqid,res"#"qseqid,qaccn,sseqid,saccn,[qstart,qend,sstart,send...]"
qfastas = get_masked_fastas(qbed)
sfastas = get_masked_fastas(sbed) if qbed.filename != sbed.filename else qfastas
pairs = [True]
_get_pair_gen = get_pair(pairs_file , pair_fmt, sbed, qbed)
# need this for parallization stuff.
def get_pair_gen():
try: return _get_pair_gen.next()
except StopIteration: return None
while any(pairs):
pairs = [get_pair_gen() for i in range(ncpu)]
# this helps in parallelizing.
def get_cmd(pair):
if pair is None: return None
sfeat, qfeat = pair
#if qfeat['accn'] != "Bradi4g01820": return None
#print >>sys.stderr, qfeat, sfeat
qfasta = qfastas[qfeat['seqid']]
sfasta = sfastas[sfeat['seqid']]
sstart, sstop = sfeat['start'], sfeat['end'] #region gets no padding
qstart, qstop = grab_flanking_region(qfeat, sfeat) # sfeat here is the final table with sfeat info from qfeat dict
m = sstop - sstart
n = qstop - qstart
# if (m*n) >= 812045000: # if the database and query is large keep e_value at 2.11 else change it to something smaller
# e_value = 2.11
# else:
e_value = m*n*(2**(-28.51974)) # bit score above 15/15 noise
assert e_value > 0
cmd = bl2seq % dict(qfasta=qfasta, sfasta=sfasta, qstart=qstart,
sstart=sstart, qstop=qstop, sstop=sstop, e_value=e_value)
return cmd, qfeat, sfeat
cmds = [c for c in map(get_cmd, [l for l in pairs if l]) if c]
results = (r for r in pool.map(commands.getoutput, [c[0] for c in cmds]))
#results = (r for r in map(commands.getoutput, [c[0] for c in cmds]))
for res, (cmd, qfeat, sfeat) in zip(results, cmds):
if not res.strip(): continue
print >>sys.stderr, "%s %s" % (qfeat["accn"], sfeat["accn"]),
orient = qfeat['strand'] == sfeat['strand'] and 1 or -1
cnss = parse_blast(res, orient, qfeat, sfeat, qbed, sbed)
print >>sys.stderr, "(%i)" % len(cnss)
if len(cnss) == 0: continue
qname, sname = qfeat['accn'], sfeat['accn']
#urls = url_params(cnss, qfeat['seqid'], sfeat['seqid'], qfeat['ORG2_qfeat'])
print >> fcnss, "%s,%s,%s,[%s,%s],%s,%s" % (qname, qfeat['seqid'], sname, sfeat['qleft_gene'], sfeat['qright_gene'], sfeat['seqid'],
",".join(map(lambda l: ",".join(map(str,l)), cnss)))
return None
def main(qbed, sbed,cns_bed, pairs_file, qpad, spad, pair_fmt, blast_path, mask='F', ncpu=8):
"""main runner for finding cnss"""
pool = Pool(ncpu)
bl2seq = "%s " % blast_path + \
"-p blastn -D 1 -E 2 -q -2 -r 1 -G 5 -W 7 -F %s " % mask + \
" -e %(e_value).2f -i %(qfasta)s -j %(sfasta)s \
-I %(qstart)d,%(qstop)d -J %(sstart)d,%(sstop)d | grep -v '#' \
| grep -v 'WARNING' | grep -v 'ERROR' "
fcnss = sys.stdout
print >> fcnss, "#qseqid,qaccn,sseqid,saccn,[qstart,qend,sstart,send,evalue...]"
qfastas = get_masked_fastas(qbed)
sfastas = get_masked_fastas(sbed) if qbed.filename != sbed.filename else qfastas
pairs = [True]
_get_pair_gen = get_pair(pairs_file, pair_fmt, cns_bed, sbed)
# need this for parallization stuff.
def get_pair_gen():
try: return _get_pair_gen.next()
except StopIteration: return None
while any(pairs):
pairs = [get_pair_gen() for i in range(ncpu)]
# this helps in parallelizing.
def get_cmd(pair):
if pair is None: return None
qfeat, sfeat = pair
#if qfeat['accn'] != "Bradi4g01820": return None
#print >>sys.stderr, qfeat, sfeat
qfasta = qfastas[qfeat['seqid']]
sfasta = sfastas[sfeat['seqid']]
qstart, qstop = max(qfeat['start'] - qpad, 1), qfeat['end'] + qpad
sstart, sstop = max(sfeat['start'] - spad, 1), sfeat['end'] + spad
assert qstop - qstart > 2 * qpad or qstart == 1, (qstop, qstart)
assert sstop - sstart > 2 * spad or sstart == 1, (sstop, sstart)
#m = qstop - qstart
#n = sstop - sstart
#e_value = m*n*(2**(-28.51974)) # bit score above 15/15 noise
#assert e_value > 0
cmd = bl2seq % dict(qfasta=qfasta, sfasta=sfasta, qstart=qstart,
sstart=sstart, qstop=qstop, sstop=sstop,
e_value=30)
return cmd, qfeat, sfeat
cmds = [c for c in map(get_cmd, [l for l in pairs if l]) if c]
results = (r for r in pool.map(commands.getoutput, [c[0] for c in cmds]))
#results = (r for r in map(commands.getoutput, [c[0] for c in cmds]))
for res, (cmd, qfeat, sfeat) in zip(results, cmds):
if not res.strip(): continue
print >>sys.stderr, "%s %s" % (qfeat["accn"], sfeat['accn']),
orient = qfeat['strand'] == sfeat['strand'] and 1 or -1
cnss = parse_blast(res, orient, qfeat, sfeat, cns_bed, sbed, qpad, spad)
print >>sys.stderr, "(%i)" % len(cnss)
if len(cnss) == 0: continue
qname, sname = qfeat['accn'], sfeat['accn']
print >> fcnss, "%s,%s,%s,%s,%s" % (qfeat['seqid'], qname, sfeat['seqid'], sname,
",".join(map(lambda l: ",".join(map(str,l)),cnss)))
return None
def main(cns_file,qdups_path,sdups_path,pair_file,fmt,qbed,sbed,qpad,spad,blast_path,mask='F',ncpu=8):
pool = Pool(ncpu)
bl2seq = "%s " % blast_path + \
"-p blastn -D 1 -E 2 -q -2 -r 1 -G 5 -W 7 -F %s " % mask + \
" -e %(e_value).2f -i %(qfasta)s -j %(sfasta)s \
-I %(qstart)d,%(qstop)d -J %(sstart)d,%(sstop)d | grep -v '#' \
| grep -v 'WARNING' | grep -v 'ERROR' "
qfastas = get_masked_fastas(qbed)
sfastas = get_masked_fastas(sbed) if qbed.filename != sbed.filename else qfastas
################# file paths #####################
qnolocaldups_path = qbed.path.split(".")[0] + ".all.nolocaldups.bed"
snolocaldups_path = sbed.path.split(".")[0] + ".all.nolocaldups.bed"
qlocaldups_path = qbed.path.split(".")[0] + ".all.localdups"
slocaldups_path = sbed.path.split(".")[0] + ".all.localdups"
npair_file,nqlocaldups,nslocaldups, ncns_file = map(make_copy_of_file,[pair_file,qlocaldups_path,slocaldups_path,cns_file])
##########################################
qdups = parse_dups(qdups_path)
sdups = parse_dups(sdups_path)
dups,rdups = get_pairs(pair_file,fmt,qdups,sdups)
print len(dups), len(rdups)
ldups = get_large_dups(dups,qdups,sdups)
rdups_dic = defaultdict(dict)
rdups_both = [(qparent,sparent) for qparent,sparent in dups if qparent in rdups and sparent in rdups]
for (qparent,sparent) in dups:
if skip_pair(qparent,sparent,rdups,rdups_both,ldups):continue
cnss_size = []
qfeat_dups = get_all_dups(qdups,qparent)
sfeat_dups = get_all_dups(sdups,sparent)
pairs = [True]
_get_dups_gen = get_dups(qfeat_dups,sfeat_dups,qbed,sbed)
def get_dups_gen():
try: return _get_dups_gen.next()
except StopIteration: return None
while any(pairs):
cnss_dups = []
pairs = [get_dups_gen() for i in range(ncpu)]
###this is for parellization#########
spad_map = [spad] * len(pairs)
qpad_map = [qpad] * len(pairs)
sfastas_map = [sfastas] * len(pairs)
qfastas_map = [qfastas] * len(pairs)
bl2seq_map = [bl2seq] * len(pairs)
###################################
cmds = [c for c in map(get_cmd, [l for l in pairs if l],
bl2seq_map,qfastas_map,sfastas_map,qpad_map,spad_map) if c]
results = (r for r in pool.map(commands.getoutput, [c[0] for c in cmds]))
for res, (cmd, qfeat, sfeat) in zip(results, cmds):
orient = qfeat['strand'] == sfeat['strand'] and 1 or -1
if not res.strip(): cnss = []
else: cnss = parse_blast(res, orient, qfeat, sfeat, qbed, sbed, qpad,spad)
print >>sys.stderr, "(%i)" % len(cnss)
cnss_fmt = ",".join(map(lambda l: ",".join(map(str,l)),cnss))
cnss_size.append((len(cnss)*-1,qfeat["start"],sfeat["start"],qfeat["accn"],sfeat["accn"],cnss_fmt))
pairs = [pairs[-1]]
######################################################################
if qparent in rdups:
if (qparent,sparent) in rdups_dic[qparent].keys(): logging.info((qparent,sparent))
rdups_dic[qparent].update({(qparent,sparent):cnss_size})
elif sparent in rdups:
if (qparent,sparent) in rdups_dic[sparent].keys(): logging.info((qparent,sparent))
rdups_dic[sparent].update({(qparent,sparent):cnss_size})
else:
cnss_size.sort()
cns_number,qfeat_start,sfeat_start,qaccn,saccn,largest_cnss = cnss_size[0]
qfeat = qbed.accn(qaccn)
sfeat = sbed.accn(saccn)
print >>sys.stderr, "FINAL: {0},{1},{2}".format(qaccn,saccn,cns_number)
write_new_dups(npair_file,ncns_file,nqlocaldups,nslocaldups,cnss_size,qparent,sparent,qfeat,sfeat,qdups,sdups)
best_reps = best_repeats(rdups_dic)
for dparents in best_reps.keys():
#print dparents
qparent,sparent = dparents
#print parents,best_reps[parents]
### one or list? cnss[0]?
cns_number,qfeat_start, sfeat_start,qaccn,saccn,largest_cnss = best_reps[dparents]
qfeat= qbed.accn(qaccn)
sfeat = sbed.accn(saccn)
write_new_dups(npair_file,ncns_file,nqlocaldups,nslocaldups,[best_reps[dparents]],qparent,sparent,qfeat,sfeat,qdups,sdups)
write_nolocaldups(qbed.path,nqlocaldups,"{0}.all.nolocaldups.bed.local".format(qbed.path.split(".")[0]))
write_nolocaldups(sbed.path,nslocaldups,"{0}.all.nolocaldups.bed.local".format(sbed.path.split(".")[0]))
pairs_to_qa(npair_file,'pair',"{0}.all.nolocaldups.bed.local".format(qbed.path.split(".")[0]),"{0}.all.nolocaldups.bed.local".format(sbed.path.split(".")[0]),"{0}.raw.filtered.local".format(options.pairs.split(".")[0]))
import os, shutil, subprocess, zipfile, random
from processing import Pool
from zipfile import ZipFile
from config import *
def unzip(params):
base, ext = params
zf = zipfile.ZipFile(rawDir+'/'+base+'.'+ext, 'r')
for name in zf.namelist():
if name != 'PIC/':
print name
zf.extract(name, extractDir)
files = []
for f in os.listdir(rawDir):
if(f.endswith('exe')):
base, ext = f.split('.')
if(len(base) == 3):
files.append((base,ext))
p=Pool()
p.map(unzip, files)
def test():
print 'cpuCount() = %d\n' % cpuCount()
#
# Create pool
#
PROCESSES = 4
print 'Creating pool with %d processes\n' % PROCESSES
pool = Pool(PROCESSES)
#
# Tests
#
TASKS = [(mul, (i, 7)) for i in range(10)] + \
[(plus, (i, 8)) for i in range(10)]
results = [pool.applyAsync(calculate, t) for t in TASKS]
imap_it = pool.imap(calculatestar, TASKS)
imap_unordered_it = pool.imapUnordered(calculatestar, TASKS)
print 'Ordered results using pool.applyAsync():'
for r in results:
print '\t', r.get()
print
print 'Ordered results using pool.imap():'
for x in imap_it:
print '\t', x
print
print 'Unordered results using pool.imapUnordered():'
for x in imap_unordered_it:
print '\t', x
print
print 'Ordered results using pool.map() --- will block till complete:'
for x in pool.map(calculatestar, TASKS):
print '\t', x
print
#
# Simple benchmarks
#
N = 100000
print 'def pow3(x): return x**3'
t = time.time()
A = map(pow3, xrange(N))
print '\tmap(pow3, xrange(%d)):\n\t\t%s seconds' % \
(N, time.time() - t)
t = time.time()
B = pool.map(pow3, xrange(N))
print '\tpool.map(pow3, xrange(%d)):\n\t\t%s seconds' % \
(N, time.time() - t)
t = time.time()
C = list(pool.imap(pow3, xrange(N), chunksize=N//8))
print '\tlist(pool.imap(pow3, xrange(%d), chunksize=%d)):\n\t\t%s' \
' seconds' % (N, N//8, time.time() - t)
assert A == B == C, (len(A), len(B), len(C))
print
L = [None] * 1000000
print 'def noop(x): pass'
print 'L = [None] * 1000000'
t = time.time()
A = map(noop, L)
print '\tmap(noop, L):\n\t\t%s seconds' % \
(time.time() - t)
t = time.time()
B = pool.map(noop, L)
print '\tpool.map(noop, L):\n\t\t%s seconds' % \
(time.time() - t)
t = time.time()
C = list(pool.imap(noop, L, chunksize=len(L)//8))
print '\tlist(pool.imap(noop, L, chunksize=%d)):\n\t\t%s seconds' % \
(len(L)//8, time.time() - t)
assert A == B == C, (len(A), len(B), len(C))
print
del A, B, C, L
#
# Test error handling
#
print 'Testing error handling:'
try:
print pool.apply(f, (5,))
except ZeroDivisionError:
print '\tGot ZeroDivisionError as expected from pool.apply()'
else:
raise AssertionError, 'expected ZeroDivisionError'
try:
print pool.map(f, range(10))
except ZeroDivisionError:
print '\tGot ZeroDivisionError as expected from pool.map()'
else:
raise AssertionError, 'expected ZeroDivisionError'
try:
print list(pool.imap(f, range(10)))
except ZeroDivisionError:
print '\tGot ZeroDivisionError as expected from list(pool.imap())'
else:
raise AssertionError, 'expected ZeroDivisionError'
it = pool.imap(f, range(10))
for i in range(10):
try:
x = it.next()
except ZeroDivisionError:
if i == 5:
pass
except StopIteration:
break
else:
if i == 5:
raise AssertionError, 'expected ZeroDivisionError'
assert i == 9
print '\tGot ZeroDivisionError as expected from IMapIterator.next()'
print
#
# Testing timeouts
#
print 'Testing ApplyResult.get() with timeout:',
res = pool.applyAsync(calculate, TASKS[0])
while 1:
sys.stdout.flush()
try:
sys.stdout.write('\n\t%s' % res.get(0.02))
break
except TimeoutError:
sys.stdout.write('.')
print
print
print 'Testing IMapIterator.next() with timeout:',
it = pool.imap(calculatestar, TASKS)
while 1:
sys.stdout.flush()
try:
sys.stdout.write('\n\t%s' % it.next(0.02))
except StopIteration:
break
except TimeoutError:
sys.stdout.write('.')
print
print
#
# Testing callback
#
print 'Testing callback:'
A = []
B = [56, 0, 1, 8, 27, 64, 125, 216, 343, 512, 729]
r = pool.applyAsync(mul, (7, 8), callback=A.append)
r.wait()
r = pool.mapAsync(pow3, range(10), callback=A.extend)
r.wait()
if A == B:
print '\tcallbacks succeeded\n'
else:
print '\t*** callbacks failed\n\t\t%s != %s\n' % (A, B)
#
# Check there are no outstanding tasks
#
assert not pool._cache, 'cache = %r' % pool._cache
#
# Check close() methods
#
print 'Testing close():'
for worker in pool._pool:
assert worker.isAlive()
result = pool.applyAsync(time.sleep, [0.5])
pool.close()
pool.join()
assert result.get() is None
for worker in pool._pool:
assert not worker.isAlive()
print '\tclose() succeeded\n'
#
# Check terminate() method
#
print 'Testing terminate():'
pool = Pool(2)
ignore = pool.apply(pow3, [2])
results = [pool.applyAsync(time.sleep, [10]) for i in range(10)]
pool.terminate()
pool.join()
for worker in pool._pool:
assert not worker.isAlive()
print '\tterminate() succeeded\n'
#
# Check garbage collection
#
print 'Testing garbage collection:'
pool = Pool(2)
processes = pool._pool
ignore = pool.apply(pow3, [2])
results = [pool.applyAsync(time.sleep, [10]) for i in range(10)]
del results, pool
time.sleep(0.2)
for worker in processes:
assert not worker.isAlive()
print '\tgarbage collection succeeded\n'
def main(qbed,sbed,missed_pairs, ncpu):
"""run tblastx on missed pairs..."""
#print >>sys.stderr,ncpu
ncpu = int(ncpu)
pool = Pool(ncpu)
pairs_file = get_pairs_file(missed_pairs)
print >>sys.stdout, "#hit,ref_gene,blastn_introns,blastx_hits, blastx_gene_hits, blastx_frame, blastn_gaps, blastx_gaps,orf_perdiction,orf_blastx,frame_shift"
blastn = "/Users/gturco/blast-2.2.25/bin/bl2seq -p blastn -G 5 -E 2 -W 7 -q -2 -e 0.001 -D 1 -i {0} -j {1} -I {2},{3} -J {4},{5} | grep -v '#' | grep -v 'WARNING' | grep -v 'ERROR' "
qfastas = split_fastas(qbed)#MASK CODING
sfastas = get_mask_non_cds(sbed) #mask noncoding
pairs = [True]
_get_pair_gen = get_pair(pairs_file,"pair", qbed,sbed)
def get_pair_gen():
try: return _get_pair_gen.next()
except StopIteration: return None
while any(pairs):
pairs = [get_pair_gen() for i in range(ncpu)]
def get_blastn_cmd(pair):
"""creates the dictionary values used to fill in blast cmd"""
if pair is None: return None
hit, gene = pair
hstart, hstop = abs(3000 - hit['start']), (3000 + hit['end'])
# double check fasta to make sure i dont need to add or remove one
gstart,gstop = gene['start'],gene['end']
# checks the entire gene...
query_file = qfastas[hit['seqid']]
subject_file = sfastas[gene['seqid']]
blastn_cmd = blastn.format(query_file, subject_file, hstart, hstop, gstart, gstop)
#print >> sys.stderr,'{0},{1},{2}'.format(hit['accn'],gene['accn'],cmd)
return blastn_cmd,hit, gene
cmds = [c for c in map(get_blastn_cmd, [l for l in pairs if l]) if c]
#print >>sys.stderr, "results: {0}".format(cmds[0][0])
results = (r for r in pool.map(commands.getoutput,[c[0] for c in cmds]))
for res, (cmd, hit, gene) in zip(results,cmds):
print >>sys.stderr, "CMD: {0},{1}".format(gene['accn'],hit['accn'])
d,no_res = group_cds(res, gene)
gap_list =[]
intron_list = []
hit['locs'] = []
if no_res == True: continue
for group_key in d.keys():
exon_hits = d[group_key]
non_crossing = remove_crossing_hits(exon_hits,hit,gene)
if len(non_crossing) > 1:
gaps,hstart,hend =bites(non_crossing)
gap_list.append(sum(gaps))
elif len(non_crossing) == 1:
# print >>sys.stderr, non_crossing
[(hstart,hend,sstart,send,evalue)] = non_crossing
if len(non_crossing) >= 1:
intron_list.append(group_key[0])
hit['locs'].append((hstart,hend))
hit['locs'].sort()
#print >>sys.stderr, "hit_loc : {0}".format(hit['locs'])
if len(hit['locs']) < 1: continue
orf_prediction = find_orf(qbed,hit)
introns = "{0}/{1}".format(len(intron_list),len(gene['locs']))
gap_totaln = sum(gap_list)
# new hit locs made from blastn res
hit_percent, gene_percent, frame_percent,frame_shift, best_frame, gap_total,orf_start= protein_parse(hit,gene,sbed,qbed)
orf_start = abs(min(hit['locs'][0]) + int(orf_start))
w ="{0},{1},{2},{3},{4},{5},{6},{7},{8},{9},{10}".format(hit['accn'],gene['accn'],introns,hit_percent,gene_percent, frame_percent,gap_totaln,gap_total,orf_prediction,orf_start,frame_shift)
print >>sys.stdout, w
import numpy as np
import math
def f(x):
print x
y = [1]*10000000
[math.exp(i) for i in y]
def g(x):
print x
y = np.ones(10000000)
np.exp(y)
from handythread import foreach
from processing import Pool
from timings import f,g
def fornorm(f,l):
for i in l:
f(i)
%timeit fornorm(f,range(10))
%timeit foreach(g,range(100),threads=2)
%timeit foreach(f,range(10),threads=2)
p = Pool(2)
%timeit p.map(g,range(100))
%timeit p.map(f,range(100))
def main(qbed, sbed, pairs_file, pad, pair_fmt, mask='F', ncpu=8):
"""main runner for finding cnss"""
pool = Pool(options.ncpu)
bl2seq = "/usr/bin/bl2seq " \
"-p blastn -D 1 -E 2 -q -2 -r 1 -G 5 -W 7 -F %s " % mask + \
" -Y 812045000 -d 26195 -e 2.11 -i %(qfasta)s -j %(sfasta)s \
-I %(qstart)d,%(qstop)d -J %(sstart)d,%(sstop)d | grep -v '#' \
| grep -v 'WARNING' | grep -v 'ERROR' "
fcnss = sys.stdout
print >> fcnss, "#qseqid,qaccn,sseqid,saccn,[qstart,qend,sstart,send...]"
qfastas = get_masked_fastas(qbed)
sfastas = get_masked_fastas(sbed) if qbed.filename != sbed.filename else qfastas
pairs = [True]
_get_pair_gen = get_pair(pairs_file, pair_fmt, qbed, sbed)
# need this for parallization stuff.
def get_pair_gen():
try: return _get_pair_gen.next()
except StopIteration: return None
while any(pairs):
pairs = [get_pair_gen() for i in range(ncpu)]
# this helps in parallelizing.
def get_cmd(pair):
if pair is None: return None
qfeat, sfeat = pair
#if qfeat['accn'] != "Bradi4g01820": return None
#print >>sys.stderr, qfeat, sfeat
qfasta = qfastas[qfeat['seqid']]
sfasta = sfastas[sfeat['seqid']]
qstart, qstop = max(qfeat['start'] - pad, 1), qfeat['end'] + pad
sstart, sstop = max(sfeat['start'] - pad, 1), sfeat['end'] + pad
assert qstop - qstart > 2 * pad or qstart == 1, (qstop, qstart)
assert sstop - sstart > 2 * pad or sstart == 1, (sstop, sstart)
cmd = bl2seq % dict(qfasta=qfasta, sfasta=sfasta, qstart=qstart,
sstart=sstart, qstop=qstop, sstop=sstop)
return cmd, qfeat, sfeat
cmds = [c for c in map(get_cmd, [l for l in pairs if l]) if c]
results = (r for r in pool.map(commands.getoutput, [c[0] for c in cmds]))
#results = (r for r in map(commands.getoutput, [c[0] for c in cmds]))
for res, (cmd, qfeat, sfeat) in zip(results, cmds):
if not res.strip(): continue
print >>sys.stderr, "%s %s" % (qfeat["accn"], sfeat['accn']),
orient = qfeat['strand'] == sfeat['strand'] and 1 or -1
cnss = parse_blast(res, orient, qfeat, sfeat, qbed, sbed, pad)
print >>sys.stderr, "(%i)" % len(cnss)
if len(cnss) == 0: continue
qname, sname = qfeat['accn'], sfeat['accn']
print >> fcnss, "%s,%s,%s,%s,%s" % (qfeat['seqid'], qname, sfeat['seqid'], sname,
",".join(map(lambda l: ",".join(map(str,l)),cnss)))
return None
def main(cns_file,
qdups_path,
sdups_path,
pair_file,
fmt,
qbed,
sbed,
qpad,
spad,
blast_path,
unmasked_fasta,
mask='F',
ncpu=8):
pool = Pool(ncpu)
bl2seq = "%s " % blast_path + \
"-p blastn -D 1 -E 2 -q -2 -r 1 -G 5 -W 7 -F %s " % mask + \
" -e %(e_value).2f -i %(qfasta)s -j %(sfasta)s \
-I %(qstart)d,%(qstop)d -J %(sstart)d,%(sstop)d | grep -v '#' \
| grep -v 'WARNING' | grep -v 'ERROR' "
qfastas = get_masked_fastas(qbed)
sfastas = get_masked_fastas(
sbed) if qbed.filename != sbed.filename else qfastas
################# file paths #####################
qnolocaldups_path = qbed.path.split(".")[0] + ".nolocaldups.bed"
snolocaldups_path = sbed.path.split(".")[0] + ".nolocaldups.bed"
qlocaldups_path = qbed.path.split(".")[0] + ".localdups"
slocaldups_path = sbed.path.split(".")[0] + ".localdups"
npair_file, nqlocaldups, nslocaldups, ncns_file = map(
make_copy_of_file,
[pair_file, qlocaldups_path, slocaldups_path, cns_file])
##########################################
qdups = parse_dups(qdups_path)
sdups = parse_dups(sdups_path)
dups, rdups = get_pairs(pair_file, fmt, qdups, sdups)
print len(dups), len(rdups)
ldups = get_large_dups(dups, qdups, sdups)
rdups_dic = defaultdict(dict)
rdups_both = [(qparent, sparent) for qparent, sparent in dups
if qparent in rdups and sparent in rdups]
for (qparent, sparent) in dups:
if skip_pair(qparent, sparent, rdups, rdups_both, ldups): continue
cnss_size = []
qfeat_dups = get_all_dups(qdups, qparent)
sfeat_dups = get_all_dups(sdups, sparent)
pairs = [True]
_get_dups_gen = get_dups(qfeat_dups, sfeat_dups, qbed, sbed)
def get_dups_gen():
try:
return _get_dups_gen.next()
except StopIteration:
return None
while any(pairs):
cnss_dups = []
pairs = [get_dups_gen() for i in range(ncpu)]
###this is for parellization#########
spad_map = [spad] * len(pairs)
qpad_map = [qpad] * len(pairs)
sfastas_map = [sfastas] * len(pairs)
qfastas_map = [qfastas] * len(pairs)
bl2seq_map = [bl2seq] * len(pairs)
###################################
cmds = [
c for c in map(get_cmd, [l for l in pairs if l], bl2seq_map,
qfastas_map, sfastas_map, qpad_map, spad_map)
if c
]
results = (
r for r in pool.map(commands.getoutput, [c[0] for c in cmds]))
for res, (cmd, qfeat, sfeat) in zip(results, cmds):
orient = qfeat['strand'] == sfeat['strand'] and 1 or -1
if not res.strip(): cnss = []
else:
cnss = parse_blast(res, orient, qfeat, sfeat, qbed, sbed,
qpad, spad, unmasked_fasta)
print >> sys.stderr, "(%i)" % len(cnss)
cnss_fmt = ",".join(map(lambda l: ",".join(map(str, l)), cnss))
cnss_size.append(
(len(cnss) * -1, qfeat["start"], sfeat["start"],
qfeat["accn"], sfeat["accn"], cnss_fmt))
pairs = [pairs[-1]]
######################################################################
if qparent in rdups:
if (qparent, sparent) in rdups_dic[qparent].keys():
logging.info((qparent, sparent))
rdups_dic[qparent].update({(qparent, sparent): cnss_size})
elif sparent in rdups:
if (qparent, sparent) in rdups_dic[sparent].keys():
logging.info((qparent, sparent))
rdups_dic[sparent].update({(qparent, sparent): cnss_size})
else:
cnss_size.sort()
cns_number, qfeat_start, sfeat_start, qaccn, saccn, largest_cnss = cnss_size[
0]
qfeat = qbed.accn(qaccn)
sfeat = sbed.accn(saccn)
print >> sys.stderr, "FINAL: {0},{1},{2}".format(
qaccn, saccn, cns_number)
write_new_dups(npair_file, ncns_file, nqlocaldups, nslocaldups,
cnss_size, qparent, sparent, qfeat, sfeat, qdups,
sdups)
best_reps = best_repeats(rdups_dic)
for dparents in best_reps.keys():
qparent, sparent = dparents
### one or list? cnss[0]?
cns_number, qfeat_start, sfeat_start, qaccn, saccn, largest_cnss = best_reps[
dparents]
qfeat = qbed.accn(qaccn)
sfeat = sbed.accn(saccn)
write_new_dups(npair_file, ncns_file, nqlocaldups, nslocaldups,
[best_reps[dparents]], qparent, sparent, qfeat, sfeat,
qdups, sdups)
write_nolocaldups(
qbed.path, nqlocaldups,
"{0}.nolocaldups.bed.local".format(qbed.path.split(".")[0]))
write_nolocaldups(
sbed.path, nslocaldups,
"{0}.nolocaldups.bed.local".format(sbed.path.split(".")[0]))
pairs_to_qa(npair_file, 'pair',
"{0}.nolocaldups.bed.local".format(qbed.path.split(".")[0]),
"{0}.nolocaldups.bed.local".format(sbed.path.split(".")[0]),
"{0}.raw.filtered.local".format(options.pairs.split(".")[0]))
print "Error: cant write the file %s.%s" % (base,ext)
else:
for line in open(extractedDir+"/"+base+"."+ext, "r"):
if len(line) > 5:
begin = 0
end = 0
length = 0
output = ""
for i in configList:
length=int(i)
end = begin + length
output += line[begin:end]+";"
begin = end
dest.write(output+"\n")
dest.close()
print "Done parsing %s.%s" % (base, ext)
else:
print "File does not exist %s.%s" % (base, ext)
files = []
for f in os.listdir(extractedDir):
if f != '.DS_Store':
base, ext = f.split('.')
if ext == 'dat' or int(ext):
files.append((base,ext))
random.shuffle(files)
p = Pool()
p.map(parse, files)