예제 #1
0
def read_compressed_eigerdata(mask,
                              filename,
                              beg,
                              end,
                              bad_pixel_threshold=1e15,
                              hot_pixel_threshold=2**30,
                              bad_pixel_low_threshold=0,
                              bad_frame_list=None,
                              with_pickle=False,
                              direct_load_data=False,
                              data_path=None,
                              images_per_file=100):
    '''
        Read already compress eiger data           
        Return 
            mask
            avg_img
            imsum
            bad_frame_list
            
    '''
    #should use try and except instead of with_pickle in the future!
    CAL = False
    if not with_pickle:
        CAL = True
    else:
        try:
            mask, avg_img, imgsum, bad_frame_list_ = pkl.load(
                open(filename + '.pkl', 'rb'))
        except:
            CAL = True
    if CAL:
        FD = Multifile(filename, beg, end)
        imgsum = np.zeros(FD.end - FD.beg, dtype=np.float)
        avg_img = np.zeros([FD.md['ncols'], FD.md['nrows']], dtype=np.float)
        imgsum, bad_frame_list_ = get_each_frame_intensityc(
            FD,
            sampling=1,
            bad_pixel_threshold=bad_pixel_threshold,
            bad_pixel_low_threshold=bad_pixel_low_threshold,
            hot_pixel_threshold=hot_pixel_threshold,
            plot_=False,
            bad_frame_list=bad_frame_list)
        avg_img = get_avg_imgc(FD,
                               beg=None,
                               end=None,
                               sampling=1,
                               plot_=False,
                               bad_frame_list=bad_frame_list_)
        FD.FID.close()

    return mask, avg_img, imgsum, bad_frame_list_
예제 #2
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def _get_mean_intensity_one_q(FD, sampling, labels):
    mi = np.zeros(int((FD.end - FD.beg) / sampling))
    n = 0
    qind, pixelist = roi.extract_label_indices(labels)
    # iterate over the images to compute multi-tau correlation
    fra_pix = np.zeros_like(pixelist, dtype=np.float64)
    timg = np.zeros(FD.md['ncols'] * FD.md['nrows'], dtype=np.int32)
    timg[pixelist] = np.arange(1, len(pixelist) + 1)
    for i in range(FD.beg, FD.end, sampling):
        (p, v) = FD.rdrawframe(i)
        w = np.where(timg[p])[0]
        pxlist = timg[p[w]] - 1
        mi[n] = np.bincount(qind[pxlist], weights=v[w], minlength=2)[1:]
        n += 1
    return mi
예제 #3
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def get_time_edge_avg_img(FD, frame_edge, show_progress=True):
    '''YG Dev Nov 14, 2017@CHX
    Get averaged img by giving FD and frame edges
    Parameters
    ----------
    FD: Multifile class
        compressed file
    frame_edge: np.array, can be created by create_time_slice( Nimg, slice_num= 3, 
        slice_width= 1, edges = None )
        e.g., np.array([[   5,    6],
           [2502, 2503],
           [4999, 5000]])
    Return:
        array: (N of frame_edge,  averaged image) , i.e., d[0] gives the first averaged image
    '''

    Nt = len(frame_edge)
    d = np.zeros(Nt, dtype=object)
    for i in range(Nt):
        t1, t2 = frame_edge[i]
        d[i] = get_avg_imgc(FD,
                            beg=t1,
                            end=t2,
                            sampling=1,
                            plot_=False,
                            show_progress=show_progress)

    return d
예제 #4
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 def get_bin_frame(self):
     FD = self.FD
     self.frames = np.zeros(
         [FD.md['ncols'], FD.md['nrows'],
          len(self.time_edge)])
     for n in tqdm(range(len(self.time_edge))):
         #print (n)
         t1, t2 = self.time_edge[n]
         #print( t1, t2)
         self.frames[:, :, n] = get_avg_imgc(FD,
                                             beg=t1,
                                             end=t2,
                                             sampling=1,
                                             plot_=False,
                                             show_progress=False)
예제 #5
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def cal_waterfallc(FD,
                   labeled_array,
                   qindex=1,
                   bin_waterfall=False,
                   waterfall_roi_size=None,
                   save=False,
                   *argv,
                   **kwargs):
    """Compute the mean intensity for each ROI in the compressed file (FD)

    Parameters
    ----------
    FD: Multifile class
        compressed file
    labeled_array : array
        labeled array; 0 is background.
        Each ROI is represented by a nonzero integer. It is not required that
        the ROI labels are contiguous
    qindex : int, qindex=1, give the first ring in SAXS geometry. NOTE: qindex=0 is non-photon pixels. 
        The ROI's to use.  
        
    bin_waterfall: if True, will bin the waterfall along y-axis
    waterfall_roi_size: the size of waterfall roi,  (x-size, y-size), if bin, will bin along y
    save: save the waterfall

    Returns
    -------
    waterfall : array
        The mean intensity of each ROI for all `images`
        Dimensions:
            len(mean_intensity) == len(index)
            len(mean_intensity[0]) == len(images)
    index : list
        The labels for each element of the `mean_intensity` list
    """
    sampling = 1

    labeled_array_ = np.array(labeled_array == qindex, dtype=np.int64)

    qind, pixelist = roi.extract_label_indices(labeled_array_)

    if labeled_array_.shape != (FD.md['ncols'], FD.md['nrows']):
        raise ValueError(
            " `image` shape (%d, %d) in FD is not equal to the labeled_array shape (%d, %d)"
            % (FD.md['ncols'], FD.md['nrows'], labeled_array_.shape[0],
               labeled_array_.shape[1]))

    # pre-allocate an array for performance
    # might be able to use list comprehension to make this faster

    watf = np.zeros([int((FD.end - FD.beg) / sampling), len(qind)])

    #fra_pix = np.zeros_like( pixelist, dtype=np.float64)

    timg = np.zeros(FD.md['ncols'] * FD.md['nrows'], dtype=np.int32)
    timg[pixelist] = np.arange(1, len(pixelist) + 1)

    #maxqind = max(qind)
    norm = np.bincount(qind)[1:]
    n = 0
    #for  i in tqdm(range( FD.beg , FD.end )):
    for i in tqdm(range(FD.beg, FD.end, sampling),
                  desc='Get waterfall for q index=%s' % qindex):
        (p, v) = FD.rdrawframe(i)
        w = np.where(timg[p])[0]
        pxlist = timg[p[w]] - 1

        watf[n][pxlist] = v[w]
        n += 1

    if bin_waterfall:
        watf_ = watf.copy()
        watf = np.zeros([watf_.shape[0], waterfall_roi_size[0]])
        for i in range(waterfall_roi_size[1]):
            watf += watf_[:, waterfall_roi_size[0] * i:waterfall_roi_size[0] *
                          (i + 1)]
        watf /= waterfall_roi_size[0]

    if save:
        path = kwargs['path']
        uid = kwargs['uid']
        np.save(path + '%s_waterfall' % uid, watf)

    return watf
예제 #6
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def mean_intensityc(FD, labeled_array, sampling=1, index=None):
    """Compute the mean intensity for each ROI in the compressed file (FD)

    Parameters
    ----------
    FD: Multifile class
        compressed file
    labeled_array : array
        labeled array; 0 is background.
        Each ROI is represented by a nonzero integer. It is not required that
        the ROI labels are contiguous
    index : int, list, optional
        The ROI's to use. If None, this function will extract averages for all
        ROIs

    Returns
    -------
    mean_intensity : array
        The mean intensity of each ROI for all `images`
        Dimensions:
            len(mean_intensity) == len(index)
            len(mean_intensity[0]) == len(images)
    index : list
        The labels for each element of the `mean_intensity` list
    """

    qind, pixelist = roi.extract_label_indices(labeled_array)

    if labeled_array.shape != (FD.md['ncols'], FD.md['nrows']):
        raise ValueError(
            " `image` shape (%d, %d) in FD is not equal to the labeled_array shape (%d, %d)"
            % (FD.md['ncols'], FD.md['nrows'], labeled_array.shape[0],
               labeled_array.shape[1]))
    # handle various input for `index`
    if index is None:
        index = list(np.unique(labeled_array))
        index.remove(0)
    else:
        try:
            len(index)
        except TypeError:
            index = [index]

        index = np.array(index)
        #print ('here')
        good_ind = np.zeros(max(qind), dtype=np.int32)
        good_ind[index - 1] = np.arange(len(index)) + 1
        w = np.where(good_ind[qind - 1])[0]
        qind = good_ind[qind[w] - 1]
        pixelist = pixelist[w]

    # pre-allocate an array for performance
    # might be able to use list comprehension to make this faster

    mean_intensity = np.zeros([int((FD.end - FD.beg) / sampling), len(index)])
    #fra_pix = np.zeros_like( pixelist, dtype=np.float64)
    timg = np.zeros(FD.md['ncols'] * FD.md['nrows'], dtype=np.int32)
    timg[pixelist] = np.arange(1, len(pixelist) + 1)
    #maxqind = max(qind)
    norm = np.bincount(qind)[1:]
    n = 0
    #for  i in tqdm(range( FD.beg , FD.end )):
    for i in tqdm(range(FD.beg, FD.end, sampling),
                  desc='Get ROI intensity of each frame'):
        (p, v) = FD.rdrawframe(i)
        w = np.where(timg[p])[0]
        pxlist = timg[p[w]] - 1
        mean_intensity[n] = np.bincount(qind[pxlist],
                                        weights=v[w],
                                        minlength=len(index) + 1)[1:]
        n += 1
    mean_intensity /= norm
    return mean_intensity, index
예제 #7
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 def _readImage(self):
     (p, v) = self._readImageRaw()
     img = np.zeros((self.md['ncols'], self.md['nrows']))
     np.put(np.ravel(img), p, v)
     return (img)
예제 #8
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def init_compress_eigerdata(images,
                            mask,
                            md,
                            filename,
                            bad_pixel_threshold=1e15,
                            hot_pixel_threshold=2**30,
                            bad_pixel_low_threshold=0,
                            nobytes=4,
                            bins=1,
                            with_pickle=True):
    '''
        Compress the eiger data 
        
        Create a new mask by remove hot_pixel
        Do image average
        Do each image sum
        Find badframe_list for where image sum above bad_pixel_threshold
        Generate a compressed data with filename
        
        if bins!=1, will bin the images with bin number as bins
    
        Header contains 1024 bytes ['Magic value', 'beam_center_x', 'beam_center_y', 'count_time', 'detector_distance', 
           'frame_time', 'incident_wavelength', 'x_pixel_size', 'y_pixel_size', 
           bytes per pixel (either 2 or 4 (Default)),
           Nrows, Ncols, Rows_Begin, Rows_End, Cols_Begin, Cols_End ]
           
        Return 
            mask
            avg_img
            imsum
            bad_frame_list
            
    '''
    fp = open(filename, 'wb')
    #Make Header 1024 bytes
    #md = images.md
    if bins != 1:
        nobytes = 8

    Header = struct.pack('@16s8d7I916x', b'Version-COMP0001',
                         md['beam_center_x'], md['beam_center_y'],
                         md['count_time'], md['detector_distance'],
                         md['frame_time'], md['incident_wavelength'],
                         md['x_pixel_size'], md['y_pixel_size'], nobytes,
                         md['pixel_mask'].shape[1], md['pixel_mask'].shape[0],
                         0, md['pixel_mask'].shape[1], 0,
                         md['pixel_mask'].shape[0])

    fp.write(Header)

    Nimg_ = len(images)
    avg_img = np.zeros_like(images[0], dtype=np.float)
    Nopix = float(avg_img.size)
    n = 0
    good_count = 0
    frac = 0.0
    if nobytes == 2:
        dtype = np.int16
    elif nobytes == 4:
        dtype = np.int32
    elif nobytes == 8:
        dtype = np.float64
    else:
        print(
            "Wrong type of nobytes, only support 2 [np.int16] or 4 [np.int32]")
        dtype = np.int32

    Nimg = Nimg_ // bins
    time_edge = np.array(
        create_time_slice(N=Nimg_, slice_num=Nimg, slice_width=bins))

    imgsum = np.zeros(Nimg)
    if bins != 1:
        print('The frames will be binned by %s' % bins)

    for n in tqdm(range(Nimg)):
        t1, t2 = time_edge[n]
        img = np.average(images[t1:t2], axis=0)
        mask &= img < hot_pixel_threshold
        p = np.where((np.ravel(img) > 0)
                     & np.ravel(mask))[0]  #don't use masked data
        v = np.ravel(np.array(img, dtype=dtype))[p]
        dlen = len(p)
        imgsum[n] = v.sum()
        if (imgsum[n] > bad_pixel_threshold) or (imgsum[n] <=
                                                 bad_pixel_low_threshold):
            #if imgsum[n] >=bad_pixel_threshold :
            dlen = 0
            fp.write(struct.pack('@I', dlen))
        else:
            np.ravel(avg_img)[p] += v
            good_count += 1
            frac += dlen / Nopix
            #s_fmt ='@I{}i{}{}'.format( dlen,dlen,'ih'[nobytes==2])
            fp.write(struct.pack('@I', dlen))
            fp.write(struct.pack('@{}i'.format(dlen), *p))
            if bins == 1:
                fp.write(
                    struct.pack('@{}{}'.format(dlen, 'ih'[nobytes == 2]), *v))
            else:
                fp.write(
                    struct.pack('@{}{}'.format(dlen, 'dd'[nobytes == 2]), *v))
        #n +=1

    fp.close()
    frac /= good_count
    print("The fraction of pixel occupied by photon is %6.3f%% " %
          (100 * frac))
    avg_img /= good_count

    bad_frame_list = np.where((np.array(imgsum) > bad_pixel_threshold) | (
        np.array(imgsum) <= bad_pixel_low_threshold))[0]
    #bad_frame_list1 = np.where( np.array(imgsum) > bad_pixel_threshold  )[0]
    #bad_frame_list2 = np.where( np.array(imgsum) < bad_pixel_low_threshold  )[0]
    #bad_frame_list =   np.unique( np.concatenate( [bad_frame_list1, bad_frame_list2]) )

    if len(bad_frame_list):
        print('Bad frame list are: %s' % bad_frame_list)
    else:
        print('No bad frames are involved.')
    if with_pickle:
        pkl.dump([mask, avg_img, imgsum, bad_frame_list],
                 open(filename + '.pkl', 'wb'))
    return mask, avg_img, imgsum, bad_frame_list
예제 #9
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def segment_compress_eigerdata(images,
                               mask,
                               md,
                               filename,
                               bad_pixel_threshold=1e15,
                               hot_pixel_threshold=2**30,
                               bad_pixel_low_threshold=0,
                               nobytes=4,
                               bins=1,
                               N1=None,
                               N2=None,
                               dtypes='images',
                               reverse=True):
    '''
    Create a compressed eiger data without header, this function is for parallel compress
    for parallel compress don't pass any non-scalar parameters
    '''

    if dtypes == 'uid':
        uid = md['uid']  #images
        detector = get_detector(db[uid])
        images = load_data(uid, detector, reverse=reverse)[N1:N2]

    print(N1, N2)

    Nimg_ = len(images)

    M, N = images[0].shape
    avg_img = np.zeros([M, N], dtype=np.float)
    Nopix = float(avg_img.size)
    n = 0
    good_count = 0
    #frac = 0.0
    if nobytes == 2:
        dtype = np.int16
    elif nobytes == 4:
        dtype = np.int32
    elif nobytes == 8:
        dtype = np.float64
    else:
        print(
            "Wrong type of nobytes, only support 2 [np.int16] or 4 [np.int32]")
        dtype = np.int32

    #Nimg =   Nimg_//bins
    Nimg = int(np.ceil(Nimg_ / bins))
    time_edge = np.array(
        create_time_slice(N=Nimg_, slice_num=Nimg, slice_width=bins))
    #print( time_edge, Nimg_, Nimg, bins, N1, N2 )
    imgsum = np.zeros(Nimg)

    if bins != 1:
        print('The frames will be binned by %s' % bins)

    fp = open(filename, 'wb')
    for n in range(Nimg):
        t1, t2 = time_edge[n]
        if bins != 1:
            img = np.array(np.average(images[t1:t2], axis=0),
                           dtype=np.float64)  #dtype=np.int32)
        else:
            img = np.array(images[t1], dtype=np.int32)
        mask &= img < hot_pixel_threshold
        p = np.where(
            (np.ravel(img) > 0) * np.ravel(mask))[0]  #don't use masked data
        v = np.ravel(np.array(img, dtype=dtype))[p]
        dlen = len(p)
        imgsum[n] = v.sum()
        if (dlen == 0) or (imgsum[n] > bad_pixel_threshold) or (
                imgsum[n] <= bad_pixel_low_threshold):
            dlen = 0
            fp.write(struct.pack('@I', dlen))
        else:
            np.ravel(avg_img)[p] += v
            good_count += 1
            fp.write(struct.pack('@I', dlen))
            fp.write(struct.pack('@{}i'.format(dlen), *p))
            if bins == 1:
                fp.write(
                    struct.pack('@{}{}'.format(dlen, 'ih'[nobytes == 2]), *v))
            else:
                fp.write(
                    struct.pack('@{}{}'.format(dlen, 'dd'[nobytes == 2]),
                                *v))  #n +=1
        del p, v, img
        fp.flush()
    fp.close()
    avg_img /= good_count
    bad_frame_list = (np.array(imgsum) > bad_pixel_threshold) | (
        np.array(imgsum) <= bad_pixel_low_threshold)
    sys.stdout.write('#')
    sys.stdout.flush()
    #del  images, mask, avg_img, imgsum, bad_frame_list
    #print( 'Should release memory here')
    return mask, avg_img, imgsum, bad_frame_list
예제 #10
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def para_compress_eigerdata(images,
                            mask,
                            md,
                            filename,
                            num_sub=100,
                            bad_pixel_threshold=1e15,
                            hot_pixel_threshold=2**30,
                            bad_pixel_low_threshold=0,
                            nobytes=4,
                            bins=1,
                            dtypes='uid',
                            reverse=True,
                            num_max_para_process=500,
                            cpu_core_number=72,
                            with_pickle=True):

    if dtypes == 'uid':
        uid = md['uid']  #images
        detector = get_detector(db[uid])
        images_ = load_data(uid, detector, reverse=reverse)[:100]
        N = len(images_)
    else:
        N = len(images)

    #print( N)

    N = int(np.ceil(N / bins))
    Nf = int(np.ceil(N / num_sub))
    if Nf > cpu_core_number:
        print(
            "The process number is larger than %s (XF11ID server core number)"
            % cpu_core_number)
        num_sub_old = num_sub
        num_sub = int(np.ceil(N / cpu_core_number))
        Nf = int(np.ceil(N / num_sub))
        print("The sub compressed file number was changed from %s to %s" %
              (num_sub_old, num_sub))
    create_compress_header(md, filename + '-header', nobytes, bins)
    #print( 'done for header here')
    results = para_segment_compress_eigerdata(
        images=images,
        mask=mask,
        md=md,
        filename=filename,
        num_sub=num_sub,
        bad_pixel_threshold=bad_pixel_threshold,
        hot_pixel_threshold=hot_pixel_threshold,
        bad_pixel_low_threshold=bad_pixel_low_threshold,
        nobytes=nobytes,
        bins=bins,
        dtypes=dtypes,
        num_max_para_process=num_max_para_process)

    res_ = np.array([results[k].get() for k in list(sorted(results.keys()))])
    imgsum = np.zeros(N)
    bad_frame_list = np.zeros(N, dtype=bool)
    good_count = 1
    for i in range(Nf):
        mask_, avg_img_, imgsum_, bad_frame_list_ = res_[i]
        imgsum[i * num_sub:(i + 1) * num_sub] = imgsum_
        bad_frame_list[i * num_sub:(i + 1) * num_sub] = bad_frame_list_
        if i == 0:
            mask = mask_
            avg_img = np.zeros_like(avg_img_)
        else:
            mask *= mask_
        if not np.sum(np.isnan(avg_img_)):
            avg_img += avg_img_
            good_count += 1

    bad_frame_list = np.where(bad_frame_list)[0]
    avg_img /= good_count

    if len(bad_frame_list):
        print('Bad frame list are: %s' % bad_frame_list)
    else:
        print('No bad frames are involved.')
    print('Combining the seperated compressed files together...')
    combine_compressed(filename, Nf, del_old=True)

    del results
    del res_
    if with_pickle:
        pkl.dump([mask, avg_img, imgsum, bad_frame_list],
                 open(filename + '.pkl', 'wb'))
    return mask, avg_img, imgsum, bad_frame_list
예제 #11
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def get_each_frame_intensityc(FD,
                              sampling=1,
                              bad_pixel_threshold=1e10,
                              bad_pixel_low_threshold=0,
                              hot_pixel_threshold=2**30,
                              plot_=False,
                              bad_frame_list=None,
                              save=False,
                              *argv,
                              **kwargs):
    '''Get the total intensity of each frame by sampling every N frames
       Also get bad_frame_list by check whether above  bad_pixel_threshold  
       
       Usuage:
       imgsum, bad_frame_list = get_each_frame_intensity(good_series ,sampling = 1000, 
                                bad_pixel_threshold=1e10,  plot_ = True)
    '''

    #print ( argv, kwargs )
    #mask &= img < hot_pixel_threshold
    imgsum = np.zeros(int((FD.end - FD.beg) / sampling))
    n = 0
    for i in tqdm(range(FD.beg, FD.end, sampling),
                  desc='Get each frame intensity'):
        (p, v) = FD.rdrawframe(i)
        if len(p) > 0:
            imgsum[n] = np.sum(v)
        n += 1

    if plot_:
        uid = 'uid'
        if 'uid' in kwargs.keys():
            uid = kwargs['uid']
        fig, ax = plt.subplots()
        ax.plot(imgsum, 'bo')
        ax.set_title('uid= %s--imgsum' % uid)
        ax.set_xlabel('Frame_bin_%s' % sampling)
        ax.set_ylabel('Total_Intensity')

        if save:
            #dt =datetime.now()
            #CurTime = '%s%02d%02d-%02d%02d-' % (dt.year, dt.month, dt.day,dt.hour,dt.minute)
            path = kwargs['path']
            if 'uid' in kwargs:
                uid = kwargs['uid']
            else:
                uid = 'uid'
            #fp = path + "uid= %s--Waterfall-"%uid + CurTime + '.png'
            fp = path + "uid=%s--imgsum-" % uid + '.png'
            fig.savefig(fp, dpi=fig.dpi)

        plt.show()

    bad_frame_list_ = np.where((np.array(imgsum) > bad_pixel_threshold) | (
        np.array(imgsum) <= bad_pixel_low_threshold))[0] + FD.beg

    if bad_frame_list is not None:
        bad_frame_list = np.unique(
            np.concatenate([bad_frame_list, bad_frame_list_]))
    else:
        bad_frame_list = bad_frame_list_

    if len(bad_frame_list):
        print('Bad frame list length is: %s' % len(bad_frame_list))
    else:
        print('No bad frames are involved.')
    return imgsum, bad_frame_list
예제 #12
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def mean_intensityc(FD,
                    labeled_array,
                    sampling=1,
                    index=None,
                    multi_cor=False):
    """Compute the mean intensity for each ROI in the compressed file (FD), support parallel computation

    Parameters
    ----------
    FD: Multifile class
        compressed file
    labeled_array : array
        labeled array; 0 is background.
        Each ROI is represented by a nonzero integer. It is not required that
        the ROI labels are contiguous
    index : int, list, optional
        The ROI's to use. If None, this function will extract averages for all
        ROIs

    Returns
    -------
    mean_intensity : array
        The mean intensity of each ROI for all `images`
        Dimensions:
            len(mean_intensity) == len(index)
            len(mean_intensity[0]) == len(images)
    index : list
        The labels for each element of the `mean_intensity` list
    """

    qind, pixelist = roi.extract_label_indices(labeled_array)
    sx, sy = (FD.rdframe(FD.beg)).shape
    if labeled_array.shape != (sx, sy):
        raise ValueError(
            " `image` shape (%d, %d) in FD is not equal to the labeled_array shape (%d, %d)"
            % (sx, sy, labeled_array.shape[0], labeled_array.shape[1]))
    # handle various input for `index`
    if index is None:
        index = list(np.unique(labeled_array))
        index.remove(0)
    else:
        try:
            len(index)
        except TypeError:
            index = [index]

        index = np.array(index)
        #print ('here')
        good_ind = np.zeros(max(qind), dtype=np.int32)
        good_ind[index - 1] = np.arange(len(index)) + 1
        w = np.where(good_ind[qind - 1])[0]
        qind = good_ind[qind[w] - 1]
        pixelist = pixelist[w]

    # pre-allocate an array for performance
    # might be able to use list comprehension to make this faster

    mean_intensity = np.zeros([int((FD.end - FD.beg) / sampling), len(index)])
    #fra_pix = np.zeros_like( pixelist, dtype=np.float64)
    timg = np.zeros(FD.md['ncols'] * FD.md['nrows'], dtype=np.int32)
    timg[pixelist] = np.arange(1, len(pixelist) + 1)
    #maxqind = max(qind)
    norm = np.bincount(qind)[1:]
    n = 0
    #for  i in tqdm(range( FD.beg , FD.end )):
    if not multi_cor:
        for i in tqdm(range(FD.beg, FD.end, sampling),
                      desc='Get ROI intensity of each frame'):
            (p, v) = FD.rdrawframe(i)
            w = np.where(timg[p])[0]
            pxlist = timg[p[w]] - 1
            mean_intensity[n] = np.bincount(qind[pxlist],
                                            weights=v[w],
                                            minlength=len(index) + 1)[1:]
            n += 1
    else:
        ring_masks = [
            np.array(labeled_array == i, dtype=np.int64)
            for i in np.unique(labeled_array)[1:]
        ]
        inputs = range(len(ring_masks))
        go_through_FD(FD)
        pool = Pool(processes=len(inputs))
        print('Starting assign the tasks...')
        results = {}
        for i in tqdm(inputs):
            results[i] = apply_async(pool, _get_mean_intensity_one_q,
                                     (FD, sampling, ring_masks[i]))
        pool.close()
        print('Starting running the tasks...')
        res = [results[k].get() for k in tqdm(list(sorted(results.keys())))]
        #return res
        for i in inputs:
            mean_intensity[:, i] = res[i]
        print('ROI mean_intensit calculation is DONE!')
        del results
        del res

    mean_intensity /= norm
    return mean_intensity, index