def __encrypt_unmapped(alignment: pysam.AlignedSegment, secret: bytes):
"""
Stream cipher encryption / decryption.
alignment + secret => encrypted_alignment
encrypted_alignment + secret => alignment
:param alignment:
:param secret:
:return: encrypter/decrypted alignment
"""
if alignment.is_unmapped:
if secret is None:
raise ValueError(
'Secret key must be present when unmapped alignments are iterated.'
)
# use 64B long hash (encrypts 256 bases)
sha512 = hashlib.sha512()
sha512.update(secret + alignment.query_name.encode())
mut_seq = cmn.stream_cipher(alignment.query_sequence,
sha512.digest())
# change and preserve quality
# TODO: maybe something else with the quality?
quality = alignment.query_qualities
alignment.query_sequence = mut_seq
alignment.query_qualities = quality
def test_sam_alignment_to_padded_alignment():
alignment = AlignedSegment()
alignment.reference_start = 0
alignment.query_sequence = 'AGCTTAGCTAGCTACCTATATCTTGGTCTTGGCCG'
alignment.cigartuples = ((0,10), (2,1), (0,25))
ref = Reference('test', 'AGCTTAGCTAAGCTACCTATATCTTGGTCTTGGCCG')
(pad_ref, pad_match, pad_query) = sam_alignment_to_padded_alignment(alignment, ref)
assert pad_ref == 'AGCTTAGCTAAGCTACCTATATCTTGGTCTTGGCCG'
assert pad_match == '|||||||||| |||||||||||||||||||||||||'
assert pad_query == 'AGCTTAGCTA-GCTACCTATATCTTGGTCTTGGCCG'
def _set_length_dependent_fields(
self,
rec: pysam.AlignedSegment,
length: int,
bases: Optional[str] = None,
quals: Optional[List[int]] = None,
cigar: Optional[str] = None,
) -> None:
"""Fills in bases, quals and cigar on a record.
If any of bases, quals or cigar are defined, they must all have the same length/query
length. If none are defined then the length parameter is used. Undefined values are
synthesize at the inferred length.
Args:
rec: a SAM record
length: the length to use if all of bases/quals/cigar are None
bases: an optional string of bases for the read
quals: an optional list of qualities for the read
cigar: an optional cigar string for the read
"""
# Do some validation to make sure all defined things have the same lengths
lengths = set()
if bases is not None:
lengths.add(len(bases))
if quals is not None:
lengths.add(len(quals))
if cigar is not None:
cig = sam.Cigar.from_cigarstring(cigar)
lengths.add(sum([elem.length_on_query for elem in cig.elements]))
if not lengths:
lengths.add(length)
if len(lengths) != 1:
raise ValueError(
"Provided bases/quals/cigar are not length compatible.")
# Fill in the record, making any parts that were not defined as params
length = lengths.pop()
rec.query_sequence = bases if bases else self._bases(length)
rec.query_qualities = quals if quals else [self.base_quality] * length
if not rec.is_unmapped:
rec.cigarstring = cigar if cigar else f"{length}M"
def _make_read_unmapped(rec: AlignedSegment) -> None:
"""Removes mapping information from a read."""
if rec.is_reverse:
quals = rec.query_qualities
quals.reverse()
rec.query_sequence = dnautils.reverse_complement(rec.query_sequence)
rec.query_qualities = quals
rec.is_reverse = False
rec.reference_id = sam.NO_REF_INDEX
rec.reference_start = sam.NO_REF_POS
rec.cigar = None
rec.mapping_quality = 0
rec.template_length = 0
rec.is_duplicate = False
rec.is_secondary = False
rec.is_supplementary = False
rec.is_proper_pair = False
rec.is_unmapped = True
