def main(job_no, coord_name, start, end, species, release, dir):
start_time = time()
if not os.path.exists(dir):
os.makedirs(dir)
LOGGER.log_file_path = dir + "/" + str(
os.path.basename(__file__)) + '_' + job_no + ".log"
LOGGER.log_args()
LOGGER.log_message(get_file_hexdigest(__file__),
label="Hex digest of script.".ljust(17))
LOGGER.log_message('Name = ' + ensembldb3.__name__ + ', version = ' +
ensembldb3.__version__,
label="Imported module".ljust(30))
account = HostAccount(*os.environ['ENSEMBL_ACCOUNT'].split())
ig_count, sequence_length = 0, 0
genome = Genome(species,
release=release,
account=account,
pool_recycle=3600)
gene_count = 0
gene_intervals = list()
genes = genome.get_features(coord_name=coord_name,
start=start,
end=end,
feature_types='gene')
for gene in genes:
if gene.location.coord_name != coord_name:
break
gene_count += 1
gene_intervals.append((gene.location.start, gene.location.end))
gene_intervals = sorted(gene_intervals, key=lambda x: x[1])
intergenic = interval_complement(gene_intervals)
intergenic_sequence = ""
for ig_interval in intergenic:
ig_count += 1
sequence_length += ig_interval[1] - ig_interval[0]
region = genome.get_region(coord_name=coord_name,
start=ig_interval[0],
end=ig_interval[1])
intergenic_sequence = intergenic_sequence + 'XXXXXXXXXX' + str(
region.seq)
LOGGER.log_message(
str(ig_count),
label='Number of integenic intervals processed'.ljust(30))
LOGGER.log_message(str(sequence_length), label='Sequence length'.ljust(30))
outfile_name = dir + '/intergenic_sequence_' + species + job_no + '.pklz'
with gzip.open(outfile_name, 'wb') as outfile:
pickle.dump(intergenic_sequence, outfile)
outfile = open(outfile_name, 'r')
LOGGER.output_file(outfile.name)
outfile.close()
duration = time() - start_time
LOGGER.log_message("%.2f" % (duration / 60.),
label="run duration (minutes)".ljust(30))
def main(job_no, coord_name, start, end, species, release, folder):
start_time = time()
if not os.path.exists(folder):
os.makedirs(folder)
LOGGER.log_file_path = folder + "/" + str(
os.path.basename(__file__)) + '_' + job_no + ".log"
LOGGER.log_args()
LOGGER.log_message(get_file_hexdigest(__file__),
label="Hex digest of script.".ljust(17))
LOGGER.log_message('Name = ' + ensembldb3.__name__ + ', version = ' +
ensembldb3.__version__,
label="Imported module".ljust(30))
account = HostAccount(*os.environ['ENSEMBL_ACCOUNT'].split())
dupl_introns, intron_count, sequence_length = 0, 0, 0
intron_list, human_list, species_list, intron_list = list(), list(), list(
), list()
genome = Genome(species,
release=release,
account=account,
pool_recycle=3600)
genes = genome.get_features(coord_name=coord_name,
start=start,
end=end,
feature_types='gene')
intron_sequence = 'X'
for gene in genes:
if gene.canonical_transcript.introns is None:
continue
for intron in gene.canonical_transcript.introns:
if intron in intron_list:
dupl_introns += 1
continue
intron_list.append(intron)
intron_count += 1
sequence_length += len(intron)
intron_sequence = intron_sequence + 'XXXXXXXXXX' + str(intron.seq)
outfile_name = folder + '/intronic_sequence' + species + job_no + '.pklz'
with gzip.open(outfile_name, 'wb') as outfile:
pickle.dump(intron_sequence, outfile)
outfile = open(outfile_name, 'r')
LOGGER.output_file(outfile.name)
outfile.close()
LOGGER.log_message(str(dupl_introns),
label='Number of duplicate introns rejected'.ljust(30))
LOGGER.log_message(str(intron_count),
label='Number of introns processed'.ljust(30))
LOGGER.log_message(str(sequence_length),
label='Total intron_length'.ljust(30))
duration = time() - start_time
LOGGER.log_message("%.2f" % (duration / 60.),
label="run duration (minutes)".ljust(30))
def main(job_no, infile_root, suffixes, dir):
start_time = time()
if not os.path.exists(dir):
os.makedirs(dir)
LOGGER.log_file_path = dir + "/" + str(
os.path.basename(__file__)) + '_' + job_no + ".log"
LOGGER.log_args()
LOGGER.log_message(get_file_hexdigest(__file__),
label="Hex digest of script.".ljust(17))
try:
LOGGER.log_message(str(os.environ['CONDA_DEFAULT_ENV']),
label="Conda environment.".ljust(17))
except KeyError:
pass
LOGGER.log_message('Name = ' + np.__name__ + ', version = ' +
np.__version__,
label="Imported module".ljust(30))
LOGGER.log_message('Name = ' + pd.__name__ + ', version = ' +
pd.__version__,
label="Imported module".ljust(30))
file_suffixes = suffixes.split(',')
total_dict = defaultdict(int)
counts = list()
for c in file_suffixes:
filename = dir + '/' + infile_root + c + '.pklz'
infile = open(filename, 'r')
LOGGER.input_file(infile.name)
infile.close()
with gzip.open(filename, 'rb') as chrdict:
chrdict = pickle.load(chrdict)
counts.append([c, sum(chrdict.values())])
for k in chrdict.keys():
total_dict[k] += chrdict[k]
counts = pd.DataFrame.from_records(counts)
outfile_name = dir + '/' + 'merged_context_data_' + job_no + '.pklz'
with gzip.open(outfile_name, 'wb') as outfile:
pickle.dump(total_dict, outfile)
outfile = open(outfile_name, 'r')
LOGGER.output_file(outfile.name)
outfile.close()
fname = dir + '/' + 'merge_counts_' + job_no + '.csv'
counts.to_csv(fname)
outfile = open(fname, 'r')
LOGGER.output_file(outfile.name)
outfile.close()
duration = time() - start_time
LOGGER.log_message("%.2f" % (duration / 60.),
label="Run duration (minutes)".ljust(50))
def test_read_from_written():
"""create files with different line endings dynamically"""
text = "abcdeENDedfguENDyhbnd"
with TemporaryDirectory(dir=TEST_ROOTDIR) as dirname:
for ex, lf in (
("f06597f8a983dfc93744192b505a8af9", "\n"),
("39db5cc2f7749f02e0c712a3ece12ffc", "\r\n"),
):
p = Path(dirname) / "test.txt"
data = text.replace("END", lf)
p.write_bytes(data.encode("utf-8"))
expect = get_text_hexdigest(data)
assert expect == ex, (expect, ex)
got = get_file_hexdigest(p)
assert got == expect, f"FAILED: {repr(lf)}, {(ex, got)}"
def main(job_no, folder, fname, dir):
start_time = time()
if not os.path.exists(dir):
os.makedirs(dir)
LOGGER.log_file_path = dir + "/" + str(os.path.basename(__file__)) + job_no + ".log" # change
LOGGER.log_args()
LOGGER.log_message(get_file_hexdigest(__file__), label="Hex digest of script.".ljust(17))
LOGGER.log_message('Name = ' + np.__name__ + ', version = ' + np.__version__, label="Imported module ")
LOGGER.log_message('Name = ' + pd.__name__ + ', version = ' + pd.__version__, label="Imported module ")
csv_filename = '/male_noncarrier.rmap'
male = pd.read_csv(folder + csv_filename, sep='\t')
male = male.sort_values(['chr', 'pos'])
csv_filename = '/female_noncarrier.rmap'
female = pd.read_csv(folder + csv_filename, sep='\t')
female = female.sort_values(['chr', 'pos'])
for chrom in range(1, 23):
chrom = str(chrom)
m = male[male['chr'] == 'chr' + chrom]
f = female[female['chr'] == 'chr' + chrom]
csv_name = dir + '/' + fname + chrom + '.csv'
infile = open(csv_name, 'r')
LOGGER.input_file(infile.name)
infile.close()
table = pd.read_csv(csv_name, sep=',', index_col=0)
#Note we are actually matching on hg37 coordinates used by deCODE
xtable = table.merge(m, 'left', ['chr', 'pos'], sort=True, suffixes=['_sexav', '_male'], \
indicator='indicm', validate='1:1')
xtable.rename(columns={"stdrate_sexav": "stdrate", "seqbin_sexav": "seqbin"}, inplace=True)
xtable = xtable.merge(f, 'left', ['chr', 'pos'], sort=True, suffixes=['_sexav', '_female'], \
indicator='indicf', validate='1:1')
assert np.all(xtable['indicm'] == 'both'), 'Merge error with male.'
assert np.all(xtable['indicf'] == 'both'), 'Merge error with female.'
csv_filename = 'Recombination_data/recomb_table_all_sexes_ch' + chrom + '.csv'
xtable.to_csv(csv_filename, sep=',')
outfile = open(csv_filename, 'r')
LOGGER.output_file(outfile.name)
outfile.close()
print(xtable.head())
duration = time() - start_time
LOGGER.log_message("%.2f" % (duration / 60.), label="run duration (minutes)".ljust(30))
def main_core(job_no, filename=None, n_jobs=5, context_size=9, dir='data'):
#global sequence
if not os.path.exists(dir):
os.makedirs(dir)
LOGGER.log_file_path = dir + "/" + str(
os.path.basename(__file__)) + '_' + job_no + ".log"
start_time = time()
LOGGER.log_args()
LOGGER.log_message(get_file_hexdigest(__file__),
label="Hex digest of script.".ljust(30))
LOGGER.log_message('Name = ' + re.__name__ + ', version = ' +
re.__version__,
label="Imported module".ljust(30))
infile = open(filename, 'r')
LOGGER.input_file(infile.name)
infile.close()
with gzip.open(filename, 'rb') as sequence: # i.e. intron sequence
sequence = pickle.load(sequence)
context_size = int(context_size)
contexts_generator = itertools.product('ACGT', repeat=context_size)
contexts = tuple(''.join(context) for context in contexts_generator)
concounts = list()
for context in contexts:
concount = count_single_context(context, sequence)
concounts.append(concount)
#concounts = Parallel(n_jobs=n_jobs)(delayed(count_single_context)(context) for context in contexts)
context_dict = dict(zip(contexts, concounts))
outfile_name = dir + '/context_dict_' + job_no + '.pklz'
with gzip.open(outfile_name, 'wb') as outfile:
pickle.dump(context_dict, outfile)
outfile = open(outfile_name, 'r')
LOGGER.output_file(outfile.name)
outfile.close()
LOGGER.log_message(str(len(context_dict.keys())),
label="Number of dictionary keys".ljust(30))
LOGGER.log_message(str(sum(context_dict.values())),
label="Count of contexts".ljust(30))
duration = time() - start_time
LOGGER.log_message("%.2f" % (duration / 60.),
label="run duration (minutes)".ljust(30))
def main(job_no, coord_name, start, end, species, release, var_set_id, dir):
start_time = time()
if not os.path.exists(dir):
os.makedirs(dir)
LOGGER.log_file_path = dir + "/" + str(
os.path.basename(__file__)) + '_' + job_no + ".log"
LOGGER.log_args()
LOGGER.log_message(get_file_hexdigest(__file__),
label="Hex digest of script.".ljust(17))
LOGGER.log_message('Name = ' + ensembldb3.__name__ + ', version = ' +
ensembldb3.__version__,
label="Imported module".ljust(30))
LOGGER.log_message('Name = ' + sqlalchemy.__name__ + ', version = ' +
sqlalchemy.__version__,
label="Imported module".ljust(30))
account = HostAccount(*os.environ['ENSEMBL_ACCOUNT'].split())
genome = Genome(species,
release=release,
account=account,
pool_recycle=3600)
confirm_variation_set(genome, var_set_id)
var_locations = get_variant_details(genome, coord_name, start, end)
LOGGER.log_message(str(len(var_locations)),
label='Length of var_locations list'.ljust(30))
outfile_name = dir + '/intergenic_variants_' + species + '_' + job_no + '.pklz'
with gzip.open(outfile_name, 'wb') as outfile:
pickle.dump(var_locations, outfile)
outfile = open(outfile_name, 'r')
LOGGER.output_file(outfile.name)
outfile.close()
duration = time() - start_time
LOGGER.log_message("%.2f" % (duration / 60.),
label="run duration (minutes)".ljust(30))
def main(job_no, sex, chroms, rank, dir):
start_time = time()
if not os.path.exists(dir):
os.makedirs(dir)
LOGGER.log_file_path = dir + "/" + str(
os.path.basename(__file__)) + job_no + ".log" # change
LOGGER.log_args()
LOGGER.log_message(get_file_hexdigest(__file__),
label="Hex digest of script.".ljust(17))
try:
LOGGER.log_message(str(os.environ['CONDA_DEFAULT_ENV']),
label="Conda environment.".ljust(17))
except KeyError:
pass
LOGGER.log_message('Name = ' + np.__name__ + ', version = ' +
np.__version__,
label="Imported module ")
LOGGER.log_message('Name = ' + pd.__name__ + ', version = ' +
pd.__version__,
label="Imported module ")
LOGGER.log_message('Name = ' + scipy.__name__ + ', version = ' +
scipy.__version__,
label="Imported module ")
LOGGER.log_message('Name = ' + statsmodels.__name__ + ', version = ' +
statsmodels.__version__,
label="Imported module ")
LOGGER.log_message('Name = ' + sklearn.__name__ + ', version = ' +
sklearn.__version__,
label="Imported module ")
pd.set_option('display.max_columns', None)
if chroms is None:
chroms = np.arange(1, 23).astype(str).tolist()
else:
chroms = chroms.split(',')
if rank:
LOGGER.log_message("%1d" % rank,
label="Rank of model to select (best=0).".ljust(30))
for chrom in chroms:
csv_name = dir + '/recomb_table_all_sexes_ch' + chrom + '.csv'
infile = open(csv_name, 'r')
LOGGER.input_file(infile.name)
infile.close()
data_table = pd.read_csv(csv_name, sep=',', index_col=0)
data_table = recombination.correct_missing_data(
data_table, 'LOCF', sex)
std_col = 'stdrate_' + sex
std_rates = data_table[std_col].values
variants_profiled = data_table.iloc[:, np.arange(5, 17)]
variant_counts = variants_profiled.sum(axis=1)
var_rates = variant_counts / 10000
print('\n\nChromosome number = ' + chrom)
print('Avge. mutation rate = ', np.mean(var_rates))
xvals = std_rates.reshape(-1, 1)
lmodel = LinearRegression()
lmodel.fit(xvals, var_rates)
residuals = var_rates - lmodel.predict(xvals)
sys.stdout.flush()
print('Slope, intercept, mean of residuals = ',
'%.8f' % lmodel.coef_[0], '%.8f' % lmodel.intercept_,
'%.12f' % np.mean(residuals))
orders = recombination.evaluate_ARMA_models(residuals, 10, 4)
best_order = orders[rank]
best_mdl = smt.ARMA(residuals, order=best_order).fit(method='mle',
trend='nc',
disp=0)
print(best_mdl.summary())
outfile_name = dir + '/ARMA_model_ch' + chrom + '_' + job_no + '.pklz'
recombination.save_model_details(best_mdl, outfile_name)
outfile = open(outfile_name, 'r')
LOGGER.output_file(outfile.name)
outfile.close()
duration = time() - start_time
LOGGER.log_message("%.2f" % (duration / 60.),
label="run duration (minutes)".ljust(30))
def main_core(job_no, species, varfile_name=None, intronfile_name=None, release=89, n_jobs=5, dir='data'):
global genome
if not os.path.exists(dir):
os.makedirs(dir)
LOGGER.log_file_path = dir + "/" + str(os.path.basename(__file__)) + '_' + job_no + ".log"
start_time = time()
LOGGER.log_args()
LOGGER.log_message(get_file_hexdigest(__file__), label="Hex digest of script.".ljust(25))
LOGGER.log_message('Name = ' + numpy.__name__ + ', version = ' + numpy.__version__,
label="Imported module".ljust(25))
LOGGER.log_message('Name = ' + cogent3.__name__ + ', version = ' + cogent3.__version__,
label="Imported module".ljust(25))
LOGGER.log_message('Name = ' + ensembldb3.__name__ + ', version = ' + ensembldb3.__version__,
label="Imported module".ljust(25))
account = HostAccount(*os.environ['ENSEMBL_ACCOUNT'].split())
genome = Genome(species, release=release, account=account, pool_recycle=3600)
human_seq_region_dict = dict(
{'1': 131550, '2': 131545, '3': 131551, '4': 131552, '5': 131542, '6': 131555, '7': 131559,
'8': 131560, '9': 131540, '10': 131544, '11': 131556, '12': 131546, '13': 131541,
'14': 131547, '15': 131558,
'16': 131549, '17': 131554, '18': 131548, '19': 131537, '20': 131538, '21': 131543,
'22': 131557,
'X': 131539, 'Y': 131553})
chimp_seq_region_dict = dict({"21": 212405, "7": 212407, "15": 212409, "16": 212395, "1": 212403, "17": 212411,
"18": 212410, "19": 212394, "20": 212404, "22": 212390, "3": 212392, "4": 212393,
"5": 212391, "6": 212388, "8": 212397, "9": 212396, "10": 212387, "11": 212389,
"12": 212402, "13": 212408, "14": 212401, "Y": 212406, "X": 212399})
if species == 'human':
coord_dict = dict([(v, k) for k, v in human_seq_region_dict.items()])
tag = 'human'
elif species == 'chimp':
coord_dict = dict([(v, k) for k, v in chimp_seq_region_dict.items()])
tag = 'spec_'
else:
assert False, 'Unknown species: ' + species
if varfile_name is None:
varfile_name = dir + '/var_locations_' + tag + job_no + '.pklz'
infile = open(varfile_name, 'r')
LOGGER.input_file(infile.name)
infile.close()
with gzip.open(varfile_name, 'rb') as var_details:
var_details = pickle.load(var_details)
LOGGER.log_message(str(len(var_details)), label="Number of variants read".ljust(25))
if intronfile_name is None:
intronfile_name = dir + '/all_locations_' + tag + job_no + '.pklz'
infile = open(intronfile_name, 'r')
LOGGER.input_file(infile.name)
infile.close()
with gzip.open(intronfile_name, 'rb') as intron_locs:
intron_locs = pickle.load(intron_locs)
LOGGER.log_message(str(len(intron_locs)), label="Number of introns read".ljust(25))
with gzip.open(intronfile_name, 'rb') as intron_locs:
intron_locs = pickle.load(intron_locs)
var_details, var_locs_reversed = check_variant_strand(var_details, intron_locs)
# var_details fields are: (variant name, seq region id, location, ancestral_allele, derived_allele)
item_list = Parallel(n_jobs=n_jobs)(delayed(get_contexts) (var, coord_dict) for var in var_details)
var_count_dict = Counter(item_list)
del var_count_dict[None]
outfile_name = dir + '/var_dict_' + tag + job_no + '.pklz'
with gzip.open(outfile_name, 'wb') as outfile:
pickle.dump(var_count_dict, outfile)
outfile = open(outfile_name, 'r')
LOGGER.output_file(outfile.name)
outfile.close()
duration = time() - start_time
LOGGER.log_message("%.2f" % (duration / 60.), label="run duration (minutes)".ljust(25))
def main(job_no, coord_name, start, end, species, release, var_set_id, filter,
dir):
start_time = time()
if not os.path.exists(dir):
os.makedirs(dir)
LOGGER.log_file_path = dir + "/" + str(
os.path.basename(__file__)) + '_' + job_no + ".log"
LOGGER.log_args()
LOGGER.log_message(get_file_hexdigest(__file__),
label="Hex digest of script.".ljust(17))
LOGGER.log_message('Name = ' + ensembldb3.__name__ + ', version = ' +
ensembldb3.__version__,
label="Imported module".ljust(30))
LOGGER.log_message('Name = ' + sqlalchemy.__name__ + ', version = ' +
sqlalchemy.__version__,
label="Imported module".ljust(30))
account = HostAccount(*os.environ['ENSEMBL_ACCOUNT'].split())
var_locations_list, location_list = list(), list()
dupl_introns, intron_count, bad_var_count, sequence_length = 0, 0, 0, 0
intron_list, human_list, species_list, intron_list = list(), list(), list(
), list()
genome = Genome(species,
release=release,
account=account,
pool_recycle=3600)
confirm_variation_set(genome, var_set_id)
genes = genome.get_features(coord_name=coord_name,
start=start,
end=end,
feature_types='gene')
for gene in genes:
if gene.canonical_transcript.introns is None:
continue
for intron in gene.canonical_transcript.introns:
if intron in intron_list:
dupl_introns += 1
continue
intron_list.append(intron)
intron_length = len(intron)
intron_count += 1
sequence_length += intron_length
loc = intron.location
location_list.append(
(str(loc.coord_name), loc.start, loc.end,
loc.strand)) # location.coord_name is db3util object
var_locations, bad_var_num = get_variant_details(
genome, species, intron, filter)
var_locations_list = var_locations_list + var_locations
bad_var_count += bad_var_num
LOGGER.log_message(str(dupl_introns),
label='Number of duplicate introns rejected'.ljust(30))
LOGGER.log_message(str(intron_count),
label='Number of introns processed'.ljust(30))
if species == 'human':
LOGGER.log_message(str(bad_var_count),
label='Number of rejected variants'.ljust(30))
LOGGER.log_message(str(sequence_length), label='Sequence length'.ljust(30))
LOGGER.log_message(str(len(var_locations_list)),
label='Length of var_locations list'.ljust(30))
LOGGER.log_message(str(len(var_locations_list) / sequence_length),
label='Average SNV rate'.ljust(30))
outfile_name = dir + '/var_locations_' + species + job_no + '.pklz'
with gzip.open(outfile_name, 'wb') as outfile:
pickle.dump(var_locations_list, outfile)
outfile = open(outfile_name, 'r')
LOGGER.output_file(outfile.name)
outfile.close()
outfile_name = dir + '/all_locations_' + species + job_no + '.pklz'
with gzip.open(outfile_name, 'wb') as outfile:
pickle.dump(location_list, outfile)
outfile = open(outfile_name, 'r')
LOGGER.output_file(outfile.name)
outfile.close()
duration = time() - start_time
LOGGER.log_message("%.2f" % (duration / 60.),
label="run duration (minutes)".ljust(30))
def main(job_no, chroms, draws, sex, suffixes, folder):
start_time = time()
if not os.path.exists(folder):
os.makedirs(folder)
LOGGER.log_file_path = folder + "/" + str(os.path.basename(__file__)) + job_no + ".log" # change
LOGGER.log_args()
LOGGER.log_message(get_file_hexdigest(__file__), label="Hex digest of script.".ljust(17))
try:
LOGGER.log_message(str(os.environ['CONDA_DEFAULT_ENV']), label="Conda environment.".ljust(17))
except KeyError:
pass
LOGGER.log_message('Name = ' + np.__name__ + ', version = ' + np.__version__, label="Imported module ")
LOGGER.log_message('Name = ' + pd.__name__ + ', version = ' + pd.__version__, label="Imported module ")
LOGGER.log_message('Name = ' + theano.__name__ + ', version = ' + theano.__version__, label="Imported module ")
LOGGER.log_message('Name = ' + pymc3.__name__ + ', version = ' + pymc3.__version__, label="Imported module ")
#Mutation rates per chromosome are calculated from data at Jonsson et al. Parental influence on human
# germline de novomutations in 1,548 trios from Iceland. ('Estimate mutation rates from Jonsson data.ipynb)
mrates = [1.1045541764661985e-08, 1.2481509352581898e-08, 1.254443516411994e-08, 1.2609734521720365e-08,
1.216379148788216e-08, 1.2228991967962778e-08, 1.2298304077726808e-08, 1.3325693328599174e-08,
1.0711369887343474e-08, 1.238059175011868e-08, 1.2241940318060874e-08, 1.2117457093135447e-08,
1.0174746106096945e-08, 1.0146311894484388e-08, 1.0516600482736078e-08, 1.2597261162425896e-08,
1.1681529656302903e-08, 1.1855256275211491e-08, 1.214570124735936e-08, 1.1756514975959873e-08,
8.965863348091259e-09, 9.024242643357694e-09]
result_rows = list()
columns = ['snvdens', 'p', 'q', 'alpha', 'alpha25', 'alpha975', 'beta', 'beta25', 'beta975', 'slopem',
'slopem25', 'slopem75', 'pval', 'r2', 'variance', 'variance25', 'variance975', 'mutprop', 'mutprop25',
'mutprop975', 'mutperco', 'mutperco25', 'mutperco975']
if chroms is None:
chroms = np.arange(1, 23).astype(str).tolist()
else:
chroms = chroms.split(',')
for chromplace, chrom in enumerate(chroms):
print(chromplace, 'Chromosome ', chrom)
if suffixes is None:
suffixes = len(chroms) * [""]
else:
suffixes = suffixes.split(',')
results = list()
for i, chrom in enumerate(chroms):
csv_filename = folder + '/recomb_table_all_sexes_ch' + chrom + '.csv'
infile = open(csv_filename, 'r')
LOGGER.input_file(infile.name)
infile.close()
data_table = pd.read_csv(csv_filename, sep=',', index_col=0)
data_table = recombination.correct_missing_data(data_table, 'LOCF', sex)
variants_profiled = data_table.iloc[:, np.arange(5, 17)]
variant_counts = variants_profiled.sum(axis=1)
var_rates = variant_counts / 10000
std_col = 'stdrate_' + sex
std_rates = data_table[std_col].values
print('Avge. & var. of mutation rate ', np.mean(var_rates), np.var(var_rates))
suffix = suffixes[i]
file_name = folder + '/ARMA_model_ch' + str(chrom) + suffix + '.pklz'
infile = open(file_name, 'r')
LOGGER.input_file(infile.name)
infile.close()
with gzip.open(file_name, 'rb') as model_details:
model_details = pickle.load(model_details)
trace = recombination.run_MCMC_ARMApq(std_rates, var_rates, draws, model_details)
p = model_details['order'][0]
q = model_details['order'][1]
neg_slope = sum(t['beta'] <= 0 for t in trace)
print('Chrom', chrom, 'variates with slope <=0 ', neg_slope)
print('Chrom', chrom, 'probability slope <=0 = ', neg_slope / draws)
ss_tot = np.var(var_rates)
ss_res_vars = [np.var(var_rates - t['alpha'] - t['beta'] * std_rates) for t in trace]
r2_vars = 1 - (ss_res_vars / ss_tot)
variance_variates = ss_tot - ss_res_vars
vmean = np.mean(variance_variates)
vlow = np.percentile(variance_variates, 2.5)
vhigh = np.percentile(variance_variates, 97.5)
chr_results = pd.DataFrame(variance_variates, columns=['vars'])
chr_results['chr'] = chrom
results.append(chr_results)
snv_dens = np.mean(var_rates)
intercept_mean = np.mean([t['alpha'] for t in trace])
intercept_CI_low = np.percentile([t['alpha'] for t in trace], 2.5)
intercept_CI_high = np.percentile([t['alpha'] for t in trace], 97.5)
rfunc = lambda x: (snv_dens - x) / snv_dens
mfunc = lambda x: x * mutation_rate / (snv_dens * 0.0116)
print('Proportion muts due to recomb = ', rfunc(intercept_mean),
'CIs = ', rfunc(intercept_CI_high), rfunc(intercept_CI_low))
recomb_rate = np.mean(std_rates) * 0.0116 / (100 * 1e4)
mutation_rate = mrates[i]
mutsper = (rfunc(intercept_mean) * mutation_rate) / recomb_rate
print('Mutations per CO = ', mutsper)
sys.stdout.flush()
s = summary(trace, varnames=['alpha', 'beta'])
result_row = [np.mean(var_rates), p, q,
s.loc['alpha', 'mean'], s.loc['alpha', 'hpd_2.5'], s.loc['alpha', 'hpd_97.5'],
s.loc['beta', 'mean'], s.loc['beta', 'hpd_2.5'], s.loc['beta', 'hpd_97.5'],
mfunc(s.loc['beta', 'mean']), mfunc(s.loc['beta', 'hpd_2.5']), mfunc(s.loc['beta', 'hpd_97.5']),
neg_slope / draws, np.mean(r2_vars),
vmean, vlow, vhigh, rfunc(intercept_mean), rfunc(intercept_CI_low), rfunc(intercept_CI_high),
mutsper, (rfunc(intercept_CI_high) * mutation_rate) / recomb_rate,
(rfunc(intercept_CI_low) * mutation_rate) / recomb_rate]
result_rows.append(result_row)
results_table = pd.DataFrame(result_rows, columns=columns)
outfile_name = folder + '/ARMApq_results_' + job_no + '.csv'
results_table.to_csv(outfile_name)
outfile = open(outfile_name, 'r')
LOGGER.output_file(outfile.name)
outfile.close()
results = pd.concat(results)
outfile_name = folder + '/ARMApq_variates_' + sex + '_'+ job_no + '.pklz'
with gzip.open(outfile_name, 'wb') as outfile:
pickle.dump(results, outfile)
outfile = open(outfile_name, 'r')
LOGGER.output_file(outfile.name)
outfile.close()
duration = time() - start_time
LOGGER.log_message("%.2f" % (duration / 60.), label="run duration (minutes)".ljust(30))
def main(job_no, suffix, dir):
start_time = time()
if not os.path.exists(dir):
os.makedirs(dir)
LOGGER.log_file_path = dir + "/" + str(
os.path.basename(__file__)) + '_' + job_no + ".log"
LOGGER.log_args()
LOGGER.log_message(get_file_hexdigest(__file__),
label="Hex digest of script.".ljust(17))
try:
LOGGER.log_message(str(os.environ['CONDA_DEFAULT_ENV']),
label="Conda environment.".ljust(17))
except KeyError:
pass
LOGGER.log_message('Name = ' + np.__name__ + ', version = ' +
np.__version__,
label="Imported module".ljust(30))
LOGGER.log_message('Name = ' + pd.__name__ + ', version = ' +
pd.__version__,
label="Imported module".ljust(30))
LOGGER.log_message('Name = ' + statsmodels.__name__ + ', version = ' +
statsmodels.__version__,
label="Imported module".ljust(30))
result = pd.DataFrame(
columns=['kmer', 'variance', 'Marginalise over central base?'])
#Find variance due to CpG
filename = dir + '/var_counts_1' + suffix + '.pklz'
infile = open(filename, 'r')
LOGGER.input_file(infile.name)
infile.close()
with gzip.open(filename, 'rb') as var_counts:
var_counts = pickle.load(var_counts)
filename = dir + '/context_counts_1' + suffix + '.pklz'
infile = open(filename, 'r')
LOGGER.input_file(infile.name)
infile.close()
with gzip.open(filename, 'rb') as context_counts:
context_counts = pickle.load(context_counts)
cpg_contexts = context_counts.loc['CG', 'C'] + context_counts.loc['TG', 'C'] + context_counts.loc['AG', 'C'] + \
context_counts.loc['GG', 'C'] + \
context_counts.loc['CC', 'G'] + context_counts.loc['CT', 'G'] + context_counts.loc['CA', 'G'] + \
context_counts.loc['CG', 'G']
CpG_ratio = cpg_contexts / context_counts.values.sum()
non_cpg_contexts = context_counts.values.sum() - cpg_contexts
print('Total CpG sites : ', cpg_contexts)
print('Total intronic sites : ', context_counts.values.sum())
print('Proportion CpG sites : ', CpG_ratio)
var_counts[
'C'] = var_counts['C->T'] + var_counts['C->A'] + var_counts['C->G']
var_counts[
'G'] = var_counts['G->T'] + var_counts['G->A'] + var_counts['G->C']
CpG_vars = var_counts.loc['CG', 'C'] + var_counts.loc['TG', 'C'] + var_counts.loc['AG', 'C'] + \
var_counts.loc['GG', 'C'] + \
var_counts.loc['CC', 'G'] + var_counts.loc['CT', 'G'] + var_counts.loc['CA', 'G'] + \
var_counts.loc['CG', 'G']
print('Total CpG variants : ', CpG_vars)
non_CpG_vars = var_counts.values.sum() - CpG_vars
m1 = CpG_vars / cpg_contexts
m0 = non_CpG_vars / non_cpg_contexts
m_ave = var_counts.values.sum() / context_counts.values.sum()
print('SNV density at CpG sites : ', m1)
print('SNV density at other sites: ', m0)
print('Average SNV density : ', m_ave)
t1 = CpG_ratio * (m1 - m_ave)**2
t2 = (1 - CpG_ratio) * (m0 - m_ave)**2
print('Variance due to CpG sites : ', t1 + t2)
LOGGER.log_message("%.2e" % (t1 + t2),
label="Variance due to CpG".ljust(50))
#Deal with the 1-mer case.
var_counts[
'C'] = var_counts['C->T'] + var_counts['C->A'] + var_counts['C->G']
var_counts[
'T'] = var_counts['T->C'] + var_counts['T->A'] + var_counts['T->G']
var_counts[
'A'] = var_counts['A->T'] + var_counts['A->C'] + var_counts['A->G']
var_counts[
'G'] = var_counts['G->T'] + var_counts['G->A'] + var_counts['G->C']
variant_counts = var_counts.sum(axis=0)
variant_counts = variant_counts[variant_counts.index.isin(
['C', 'T', 'A', 'G'])]
con_counts = context_counts.sum(axis=0)
mut_rates = variant_counts / con_counts
w = DescrStatsW(mut_rates, weights=con_counts, ddof=0)
row = np.array([1, w.var, 'no'])
row = pd.Series(row, index=result.columns, name=0)
result = result.append(row)
row = np.array([1, 0.0, 'yes'])
row = pd.Series(row, index=result.columns, name=1)
result = result.append(row)
i = 2
for kmer_variable in [1, 2, 3]:
filename = dir + '/var_counts_' + str(kmer_variable) + suffix + '.pklz'
infile = open(filename, 'r')
LOGGER.input_file(infile.name)
infile.close()
with gzip.open(filename, 'rb') as var_counts:
var_counts = pickle.load(var_counts)
filename = dir + '/context_counts_' + str(
kmer_variable) + suffix + '.pklz'
infile = open(filename, 'r')
LOGGER.input_file(infile.name)
infile.close()
with gzip.open(filename, 'rb') as context_counts:
context_counts = pickle.load(context_counts)
#Reformat context counts by repeating columns to match snv_densities dataframe.
extended_context_counts, cols = probpoly_bayes.reformat_context_counts(
context_counts, var_counts)
extended_context_counts.set_index(context_counts.index, inplace=True)
snv_densities = var_counts / extended_context_counts
#Calculate variance across mutation types, marginalising over the central base.
context_ratios = context_counts.div(context_counts.sum(axis=1), axis=0)
extended_context_ratios, cols = probpoly_bayes.reformat_context_counts(
context_ratios, snv_densities)
extended_context_ratios.set_index(context_ratios.index, inplace=True)
con_weighted = (snv_densities * extended_context_ratios).sum(axis=1)
u = DescrStatsW(con_weighted,
weights=context_counts.sum(axis=1),
ddof=0)
print(
'Marginalised variance due to ' + str(2 * kmer_variable + 1) +
' -mers = ', u.var)
row = np.array([2 * kmer_variable + 1, u.var, 'yes'])
row = pd.Series(row, index=result.columns, name=i)
result = result.append(row)
i += 1
#Calculate variance conditioned on kmer, not marginalising over the central base.
#Firstly we reorganise the SNV densities table so that rows correspond to kmers
# (including central base) and columns correspond to the derived base.
contexts_generator = product('ACGT', repeat=2 * kmer_variable + 1)
contexts = tuple(''.join(context) for context in contexts_generator)
kmer_densities = np.zeros((len(contexts), 4))
kmer_densities = pd.DataFrame(kmer_densities,
index=contexts,
columns=['C', 'T', 'A', 'G'])
for context in snv_densities.index:
for mut in snv_densities.columns:
ref = mut[0]
derived = mut[3]
kmer = context[0:kmer_variable] + ref + context[
kmer_variable:2 * kmer_variable]
kmer_densities.loc[kmer, derived] = snv_densities.loc[context,
mut]
#We also reorganise context counts into counts of kmers.
kmer_counts = np.zeros((len(contexts)))
kmer_counts = pd.Series(kmer_counts, index=contexts)
for kmer in kmer_counts.index:
context = kmer[0:kmer_variable] + kmer[kmer_variable +
1:2 * kmer_variable + 1]
ref = kmer[kmer_variable]
kmer_counts[kmer] = context_counts.loc[context, ref]
#Calculate the weighted variance over the full kmer.
v = DescrStatsW(kmer_densities.sum(axis=1),
weights=kmer_counts,
ddof=0)
print(
'Unmarginalised variance due to ' + str(2 * kmer_variable + 1) +
' -mers = ', v.var)
row = np.array([2 * kmer_variable + 1, v.var, 'no'])
row = pd.Series(row, index=result.columns, name=i)
result = result.append(row)
i += 1
print(result)
filename = dir + "/aggregated_results" + job_no + ".csv"
result.to_csv(filename, sep=',')
outfile = open(filename, 'r')
LOGGER.output_file(outfile.name)
outfile.close()
duration = time() - start_time
LOGGER.log_message("%.2f" % (duration / 60.),
label="Run duration (minutes)".ljust(50))
def main(job_no, var_filename, context_filename, draws, prior, nocpg, dir):
start_time = time()
if not os.path.exists(dir):
os.makedirs(dir)
LOGGER.log_file_path = dir + "/" + str(
os.path.basename(__file__)) + '_' + job_no + ".log"
LOGGER.log_args()
LOGGER.log_message(get_file_hexdigest(__file__),
label="Hex digest of script.".ljust(17))
try:
LOGGER.log_message(str(os.environ['CONDA_DEFAULT_ENV']),
label="Conda environment.".ljust(17))
except KeyError:
pass
LOGGER.log_message('Name = ' + np.__name__ + ', version = ' +
np.__version__,
label="Imported module".ljust(30))
LOGGER.log_message('Name = ' + pd.__name__ + ', version = ' +
pd.__version__,
label="Imported module".ljust(30))
draws = int(draws)
infile_name = var_filename
infile = open(infile_name, 'r')
LOGGER.input_file(infile.name)
infile.close()
with gzip.open(infile_name, 'rb') as var_data:
var_data = pickle.load(var_data)
infile_name = context_filename
infile = open(infile_name, 'r')
LOGGER.input_file(infile.name)
infile.close()
with gzip.open(infile_name, 'rb') as context_data:
context_data = pickle.load(context_data)
for kmer_variable, split in zip([1, 2, 3], [1, 4, 64]):
#for kmer_variable, split in zip([4], [1024]):
contexts = probpoly_bayes.unpack_all_contexts_to_dataframe(
kmer_variable, context_data)
duration = time() - start_time
print('Unpacked all_contexts for ', kmer_variable, '-mers at',
"%.2f" % (duration / 60.), 'minutes.')
sys.stdout.flush()
variants = probpoly_bayes.unpack_var_contexts_to_dataframe(
kmer_variable, var_data)
duration = time() - start_time
print('Unpacked var_contexts for ', kmer_variable, '-mers at',
"%.2f" % (duration / 60.), 'minutes.')
sys.stdout.flush()
outfile_name, dataset = dir + '/var_counts_' + str(
kmer_variable) + job_no + '.pklz', variants
with gzip.open(outfile_name, 'wb') as outfile:
pickle.dump(dataset, outfile)
outfile = open(outfile_name, 'r')
LOGGER.output_file(outfile.name)
outfile.close()
outfile_name, dataset = dir + '/context_counts_' + str(
kmer_variable) + job_no + '.pklz', contexts
with gzip.open(outfile_name, 'wb') as outfile:
pickle.dump(dataset, outfile)
outfile = open(outfile_name, 'r')
LOGGER.output_file(outfile.name)
outfile.close()
ncols = 12
contexts, columns = probpoly_bayes.reformat_context_counts(
contexts, variants)
w_var_samples = probpoly_bayes.calculate_variances(
variants, contexts, split, prior, draws, ncols, columns)
outfile_name = 'data/bayes_var_samples_' + job_no + '_k=' + str(
kmer_variable) + '.pklz'
with gzip.open(outfile_name, 'wb') as outfile:
pickle.dump(w_var_samples, outfile)
outfile = open(outfile_name, 'r')
LOGGER.output_file(outfile.name)
outfile.close()
del w_var_samples
duration = time() - start_time
LOGGER.log_message("%.2f" % (duration / 60.),
label="Run duration (minutes)".ljust(50))
def main(job_no, chrom, sex, species, release, dir):
start_time = time()
if not os.path.exists(dir):
os.makedirs(dir)
LOGGER.log_file_path = dir + "/" + str(
os.path.basename(__file__)) + job_no + ".log"
LOGGER.log_args()
LOGGER.log_message(get_file_hexdigest(__file__),
label="Hex digest of script.".ljust(17))
LOGGER.log_message('Name = ' + ensembldb3.__name__ + ', version = ' +
ensembldb3.__version__,
label="Imported module".ljust(30))
LOGGER.log_message('Name = ' + sqlalchemy.__name__ + ', version = ' +
sqlalchemy.__version__,
label="Imported module".ljust(30))
human_seq_region_dict = dict({
'1': 131550,
'2': 131545,
'3': 131551,
'4': 131552,
'5': 131542,
'6': 131555,
'7': 131559,
'8': 131560,
'9': 131540,
'10': 131544,
'11': 131556,
'12': 131546,
'13': 131541,
'14': 131547,
'15': 131558,
'16': 131549,
'17': 131554,
'18': 131548,
'19': 131537,
'20': 131538,
'21': 131543,
'22': 131557,
'X': 131539,
'Y': 131553
})
account = HostAccount(*os.environ['ENSEMBL_ACCOUNT'].split())
genome = Genome(species,
release=release,
account=account,
pool_recycle=3600)
variation_table = genome.VarDb.get_table('variation')
variation_feature_table = genome.VarDb.get_table('variation_feature')
var_table = variation_table.join(
variation_feature_table, variation_feature_table.c.variation_id ==
variation_table.c.variation_id)
seq_region_id = human_seq_region_dict[chrom]
file_name = sex + '_noncarrier-hg38.csv'
infile = open(file_name, 'r')
LOGGER.input_file(infile.name)
infile.close()
recombination_df = pd.read_csv(file_name, usecols=[0, 1, 2, 3, 4])
recomb_df = recombination_df.loc[lambda df: df.chr == 'chr' + chrom, :]
recomb_df = recomb_df.reset_index(drop=True)
mut_profiles = [
i[0] + '->' + i[1] for i in permutations(['C', 'T', 'A', 'G'], 2)
]
counts = np.zeros((recomb_df.shape[0], 21))
counts = pd.DataFrame(counts,
columns=mut_profiles +
['C', 'T', 'A', 'G', 'SW', 'WS', 'SS', 'WW', 'CpG'])
for index, row in recomb_df.iterrows():
midpoint = row.loc['pos38']
region = genome.get_region(coord_name=chrom,
start=midpoint - 5000,
end=midpoint + 5000,
ensembl_coord=True)
region = str(region.seq)
whereclause1 = and_(
var_table.c.variation_feature_seq_region_id == seq_region_id,
var_table.c.variation_feature_class_attrib_id == 2,
var_table.c.variation_feature_evidence_attribs.contains('370'),
var_table.c.variation_feature_variation_name.contains('rs'),
var_table.c.variation_feature_somatic == 0,
var_table.c.variation_feature_alignment_quality ==
decimal.Decimal(1),
var_table.c.variation_feature_minor_allele_freq.isnot(None),
var_table.c.variation_feature_seq_region_start > midpoint - 5000,
var_table.c.variation_feature_seq_region_start < midpoint + 5000)
var_table_ed = var_table.select(whereclause1, use_labels=True)
for snp in var_table_ed.execute():
if snp['variation_ancestral_allele'] is None:
continue
else:
ancestral_allele = snp['variation_ancestral_allele']
alleles = snp['variation_feature_allele_string']
if fnmatch(alleles, ancestral_allele + '/?'):
derived_allele = alleles[2]
elif fnmatch(alleles, '?/' + ancestral_allele):
derived_allele = alleles[0]
else:
continue
mtype = ancestral_allele + '->' + derived_allele
counts.loc[index, mtype] += 1
rel_loc = snp[
'variation_feature_seq_region_start'] - midpoint + 5000
if (region[rel_loc + 1] == 'G' and ancestral_allele == 'C' and derived_allele == 'T') or \
(region[rel_loc - 1] == 'C' and ancestral_allele == 'G' and derived_allele == 'A'):
counts.loc[index, 'CpG'] += 1
if ancestral_allele + derived_allele in ['CT', 'CA', 'GT', 'GA']:
counts.loc[index, 'SW'] += 1
if ancestral_allele + derived_allele in ['TC', 'AC', 'TG', 'AG']:
counts.loc[index, 'WS'] += 1
if ancestral_allele + derived_allele in ['CG', 'GC']:
counts.loc[index, 'SS'] += 1
if ancestral_allele + derived_allele in ['TA', 'AT']:
counts.loc[index, 'WW'] += 1
base_counts = Counter(region)
for base in ['C', 'T', 'A', 'G']:
counts.loc[index, base] = base_counts[base]
results = pd.concat([recomb_df, counts], axis=1)
csv_filename = 'recomb_table_SW_' + sex + '_ch' + chrom + '.csv'
results.to_csv(csv_filename)
outfile = open(csv_filename, 'r')
LOGGER.output_file(outfile.name)
outfile.close()
duration = time() - start_time
LOGGER.log_message("%.2f" % (duration / 60.),
label="run duration (minutes)".ljust(30))
def main(job_no, infile_name, release, dir):
start_time = time()
if not os.path.exists(dir):
os.makedirs(dir)
LOGGER.log_file_path = dir + "/" + str(
os.path.basename(__file__)) + job_no + ".log"
LOGGER.log_args()
LOGGER.log_message(get_file_hexdigest(__file__),
label="Hex digest of script.".ljust(17))
try:
LOGGER.log_message(str(os.environ['CONDA_DEFAULT_ENV']),
label="Conda environment.".ljust(17))
except KeyError:
pass
LOGGER.log_message('Name = ' + pd.__name__ + ', version = ' +
pd.__version__,
label="Imported module".ljust(30))
LOGGER.log_message('Name = ' + ensembldb3.__name__ + ', version = ' +
ensembldb3.__version__,
label="Imported module".ljust(30))
LOGGER.log_message('Name = ' + sqlalchemy.__name__ + ', version = ' +
sqlalchemy.__version__,
label="Imported module".ljust(30))
human_seq_region_dict = dict({
'1': 131550,
'2': 131545,
'3': 131551,
'4': 131552,
'5': 131542,
'6': 131555,
'7': 131559,
'8': 131560,
'9': 131540,
'10': 131544,
'11': 131556,
'12': 131546,
'13': 131541,
'14': 131547,
'15': 131558,
'16': 131549,
'17': 131554,
'18': 131548,
'19': 131537,
'20': 131538,
'21': 131543,
'22': 131557,
'X': 131539,
'Y': 131553
})
account = HostAccount(*os.environ['ENSEMBL_ACCOUNT'].split())
genome = Genome('human',
release=release,
account=account,
pool_recycle=3600)
variation_feature_table = genome.VarDb.get_table('variation_feature')
id_1KG = set([str(x) for x in range(42, 55)])
var_details = pd.read_csv(infile_name, sep=',', index_col=0)
infile = open(infile_name, 'r')
LOGGER.input_file(infile.name)
infile.close()
loc_count, match_count, count1KG, derived_mismatch_count = 0, 0, 0, 0
col_alleles, col_name, col_val_id = list(), list(), list()
for row in var_details.iterrows():
chrom = row[1].loc['chr']
chrom = chrom[3:]
seq_region_id = human_seq_region_dict[chrom]
loc38 = row[1].loc['pos38']
loc_count += 1
whereclause1 = and_(
variation_feature_table.c.seq_region_id == seq_region_id,
variation_feature_table.c.seq_region_start == loc38,
variation_feature_table.c.class_attrib_id == 2,
variation_feature_table.c.variation_name.contains("rs"),
variation_feature_table.c.somatic == 0,
variation_feature_table.c.alignment_quality == decimal.Decimal(1),
variation_feature_table.c.minor_allele_freq.isnot(None))
query = select([
variation_feature_table.c.variation_name,
variation_feature_table.c.allele_string,
variation_feature_table.c.variation_set_id
], whereclause1)
snps = list(query.execute())
if len(snps) > 0:
if len(snps) > 1:
print('More than one SNP at ', chrom, ':', loc38)
alleles = snps[0][1]
name = snps[0][0]
match_count += 1
if len(set(snps[0][2]).intersection(id_1KG)) > 0:
val_id = '1KG'
count1KG += 1
else:
val_id = 'Other'
else:
val_id = 'No match'
name = None
alleles = None
col_alleles.append(alleles)
col_name.append(name)
col_val_id.append(val_id)
assert var_details.shape[0] == len(col_val_id), 'Column mismatch.'
var_details['alleles'] = pd.Series(col_alleles)
var_details['name'] = pd.Series(col_name)
var_details['val_id'] = pd.Series(col_val_id)
LOGGER.log_message(str(loc_count), label='Variants read = ')
LOGGER.log_message(str(derived_mismatch_count),
label='Derived mismatches = ')
LOGGER.log_message(str(match_count), label='Variants matched = ')
LOGGER.log_message(str(count1KG), label='1KG Variants = ')
filename = 'data/dnms_from_PRJEB21300_matched_' + job_no + '.csv'
var_details.to_csv(filename)
outfile = open(filename, 'r')
LOGGER.output_file(outfile.name)
outfile.close()
duration = time() - start_time
LOGGER.log_message("%.2f" % (duration / 60.),
label="run duration (minutes)".ljust(30))
