def run_dataset(self, case, algorithm):
bp = "test/{}/".format(case)
test_file = bp + "test.spats.tmp"
try:
shutil.copyfile(bp + "ds.spats", test_file)
db = PairDB(test_file)
s = Spats()
db.load_run(s.run)
if not s.run.cotrans and algorithm == "native":
return
s.run.writeback_results = True
s.run.result_set_name = "test"
s.run.algorithm = algorithm
s.run.quiet = True
s.loadTargets(db)
if not s._processor.exists():
# just ignore the native test if it's not available
self.assertEqual("native", algorithm)
return
s.process_pair_db(db, batch_size = 1024) # small batch_size just to exercise multiprocessing code
msg = None
count = 0
for res in db.differing_results("test", "test_validation"):
msg = str([str(x) for x in res])
count += 1
self.assertEqual(0, count, "{} differing results: {} / {} \n{}".format(count, case, algorithm, msg))
finally:
if os.path.exists(test_file):
os.remove(test_file)
def tmut():
from spats_shape_seq import Spats
from spats_shape_seq.db import PairDB
from spats_shape_seq.diagram import diagram
bp = "/Users/jbrink/mos/tasks/1RwIBa/tmp/mutsl/"
pair_db = PairDB(bp + "ds_cmp.spats")
if True:
print "Parsing to db..."
pair_db.wipe()
pair_db.add_targets_table(bp + "mut_single.fa")
fq_name = "mut2"
pair_db.parse(bp + fq_name + "_R1.fastq", bp + fq_name + "_R2.fastq")
spatss = []
for alg in [ "find_partial", "lookup" ]:
spats = Spats(cotrans = False)
spats.run.cotrans_linker = 'CTGACTCGGGCACCAAGGAC'
spats.run.count_mutations = True
spats.run.algorithm = alg
spats.run.allowed_target_errors = 1
spats.run.adapter_b = "AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTG"
spats.run._process_all_pairs = True
spats.run.writeback_results = True
spats.run.num_workers = 1
spats.run.result_set_name = "mut_" + alg
spats.process_pair_db(pair_db)
pair_db.store_run(spats.run)
pair_db.store_counters(spats.run.result_set_name, spats.counters)
spatss.append(spats)
rdiff_func(bp + "ds_cmp.spats", "mut_find_partial", "mut_lookup", diag_spats = spatss[0])
def dbrun():
db_path = sys.argv[2]
run_name = sys.argv[3]
from spats_shape_seq import Spats
from spats_shape_seq.db import PairDB
db = PairDB(db_path)
s = Spats()
s.run.writeback_results = True
s.run.result_set_name = run_name
#s.run.resume_processing = True
s.process_pair_db(db)
def dbrun():
db_path = sys.argv[2]
run_name = sys.argv[3]
from spats_shape_seq import Spats
from spats_shape_seq.db import PairDB
db = PairDB(db_path)
s = Spats()
s.run.writeback_results = True
s.run.result_set_name = run_name
#s.run.resume_processing = True
s.process_pair_db(db)
def d5s_writeback_run():
bp = "/Users/jbrink/mos/tasks/1RwIBa/tmp/"
from spats_shape_seq.db import PairDB
pair_db = PairDB(bp + "dev_out/pairs.db")
pair_db.add_targets_table(bp + "5s/5S.fa")
from spats_shape_seq import Spats
s = Spats()
s.addTargets(bp + "5s/5S.fa")
s.writeback_results = True
s.result_set_name = "pure_python"
s.process_pair_db(pair_db)
def d5s_writeback_run():
bp = "/Users/jbrink/mos/tasks/1RwIBa/tmp/"
from spats_shape_seq.db import PairDB
pair_db = PairDB(bp + "dev_out/pairs.db")
pair_db.add_targets_table(bp + "5s/5S.fa")
from spats_shape_seq import Spats
s = Spats()
s.addTargets(bp + "5s/5S.fa")
s.writeback_results = True
s.result_set_name = "pure_python"
s.process_pair_db(pair_db)
def make_test_dataset():
bp = "/Users/jbrink/mos/tasks/1RwIBa/tmp/datasets/cotrans/data/"
from spats_shape_seq import Spats
from spats_shape_seq.db import PairDB
pair_db = PairDB(bp + "ds.spats")
pair_db.add_targets_table(bp + "../cotrans_single.fa")
pair_db.parse(bp + "med_R1.fq", bp + "med_R2.fq")
s = Spats(cotrans = True)
s.run.num_workers = 1
s.run.writeback_results = True
s.run._process_all_pairs = True
s.run.algorithm = "find_partial"
s.run.result_set_name = "test_validation"
s.process_pair_db(pair_db)
pair_db.store_run(s.run)
pair_db.store_counters('spats', s.counters)
def make_test_dataset():
bp = "/Users/jbrink/mos/tasks/1RwIBa/tmp/datasets/cotrans/data/"
from spats_shape_seq import Spats
from spats_shape_seq.db import PairDB
pair_db = PairDB(bp + "ds.spats")
pair_db.add_targets_table(bp + "../cotrans_single.fa")
pair_db.parse(bp + "med_R1.fq", bp + "med_R2.fq")
s = Spats(cotrans=True)
s.run.num_workers = 1
s.run.writeback_results = True
s.run._process_all_pairs = True
s.run.algorithm = "find_partial"
s.run.result_set_name = "test_validation"
s.process_pair_db(pair_db)
pair_db.store_run(s.run)
pair_db.store_counters('spats', s.counters)
def tags():
bp = "/Users/jbrink/mos/tasks/1RwIBa/tmp/datasets/cotrans/"
from spats_shape_seq.db import PairDB
pair_db = PairDB(bp + "db/pairs.db")
if True:
print "Parsing to db..."
pair_db.wipe()
pair_db.add_targets_table(bp + "cotrans_single.fa")
pair_db.parse(bp + "data/EJS_6_F_10mM_NaF_Rep1_GCCAAT_R1.fastq",
bp + "data/EJS_6_F_10mM_NaF_Rep1_GCCAAT_R2.fastq",
sample_size=100000)
from spats_shape_seq import Spats
from spats_shape_seq.tag import TagProcessor
from spats_shape_seq.util import reverse_complement
s = Spats()
s.run._processor_class = TagProcessor
s.run.writeback_results = True
s.run.result_set_name = "tags"
s.run.num_workers = 1
s.run.cotrans = True
s.run.cotrans_linker = 'CTGACTCGGGCACCAAGGAC'
s.loadTargets(pair_db)
s.run.allow_indeterminate = True
s.run.allowed_target_errors = 2
s.run.allowed_adapter_errors = 2
p = s._processor
for target in pair_db.targets():
p.addTagTarget(target[0], target[1])
p.addTagTarget(target[0] + "_rc", reverse_complement(target[1]))
p.addTagTarget("adapter_t_rc", reverse_complement(s.run.adapter_t))
p.addTagTarget("adapter_b", s.run.adapter_b)
if s.run.cotrans:
p.addTagTarget("linker_cotrans", s.run.cotrans_linker)
p.addTagTarget("linker_cotrans_rc",
reverse_complement(s.run.cotrans_linker))
s.process_pair_db(pair_db)
rsid = pair_db.result_set_id_for_name(s.run.result_set_name)
pair_db.count_tags(rsid)
print pair_db.tag_counts(rsid)
def tags():
bp = "/Users/jbrink/mos/tasks/1RwIBa/tmp/datasets/cotrans/"
from spats_shape_seq.db import PairDB
pair_db = PairDB(bp + "db/pairs.db")
if True:
print "Parsing to db..."
pair_db.wipe()
pair_db.add_targets_table(bp + "cotrans_single.fa")
pair_db.parse(bp + "data/EJS_6_F_10mM_NaF_Rep1_GCCAAT_R1.fastq",
bp + "data/EJS_6_F_10mM_NaF_Rep1_GCCAAT_R2.fastq",
sample_size = 100000)
from spats_shape_seq import Spats
from spats_shape_seq.tag import TagProcessor
from spats_shape_seq.util import reverse_complement
s = Spats()
s.run._processor_class = TagProcessor
s.run.writeback_results = True
s.run.result_set_name = "tags"
s.run.num_workers = 1
s.run.cotrans = True
s.run.cotrans_linker = 'CTGACTCGGGCACCAAGGAC'
s.loadTargets(pair_db)
s.run.allow_indeterminate = True
s.run.allowed_target_errors = 2
s.run.allowed_adapter_errors = 2
p = s._processor
for target in pair_db.targets():
p.addTagTarget(target[0], target[1])
p.addTagTarget(target[0] + "_rc", reverse_complement(target[1]))
p.addTagTarget("adapter_t_rc", reverse_complement(s.run.adapter_t))
p.addTagTarget("adapter_b", s.run.adapter_b)
if s.run.cotrans:
p.addTagTarget("linker_cotrans", s.run.cotrans_linker)
p.addTagTarget("linker_cotrans_rc", reverse_complement(s.run.cotrans_linker))
s.process_pair_db(pair_db)
rsid = pair_db.result_set_id_for_name(s.run.result_set_name)
pair_db.count_tags(rsid)
print pair_db.tag_counts(rsid)
def tmut():
from spats_shape_seq import Spats
from spats_shape_seq.db import PairDB
from spats_shape_seq.diagram import diagram
bp = "/Users/jbrink/mos/tasks/1RwIBa/tmp/mutsl/"
pair_db = PairDB(bp + "ds_cmp.spats")
if True:
print "Parsing to db..."
pair_db.wipe()
pair_db.add_targets_table(bp + "mut_single.fa")
fq_name = "mut2"
pair_db.parse(bp + fq_name + "_R1.fastq", bp + fq_name + "_R2.fastq")
spatss = []
for alg in ["find_partial", "lookup"]:
spats = Spats(cotrans=False)
spats.run.cotrans_linker = 'CTGACTCGGGCACCAAGGAC'
spats.run.count_mutations = True
spats.run.algorithm = alg
spats.run.allowed_target_errors = 1
spats.run.adapter_b = "AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTG"
spats.run._process_all_pairs = True
spats.run.writeback_results = True
spats.run.num_workers = 1
spats.run.result_set_name = "mut_" + alg
spats.process_pair_db(pair_db)
pair_db.store_run(spats.run)
pair_db.store_counters(spats.run.result_set_name, spats.counters)
spatss.append(spats)
rdiff_func(bp + "ds_cmp.spats",
"mut_find_partial",
"mut_lookup",
diag_spats=spatss[0])