def GetExec(self, optList, frame):
# Respond to the "embossn" type command.
self.frame = frame
plugin_exe = r"C:/mEMBOSS/water.exe"
self.outfile = r"C:\Users\francis\Documents\Monguis\BioGui\plugins\water.txt"
self.outtype = "fasta"
cline = WaterCommandline(plugin_exe, asequence=str(self.frame.paramBoxes[1].GetValue()), bsequence=str(self.frame.paramBoxes[3].GetValue()))
cline.outfile = self.outfile
cline.gapopen = self.param[7].GetValue()
cline.gapextend = self.param[9].GetValue()
if self.param[10].GetValue():
cline.similarity = True
else:
cline.similarity = False
if self.frame.abet=="AA":
cline.snucleotide = True
cline.sprotein = False
elif self.frame.abet=="DNA" or self.frame.abet=="RNA":
cline.snucleotide = True
cline.sprotein = False
if self.frame.options:
t = self.boxList[3].GetValue()
if t != '':
cline.datafile = str(t)
return str(cline)
def out():
filename1 = e1.get()
filename2 = e2.get()
outfile = e3.get()
needle_cline = WaterCommandline()
needle_cline.asequence = filename1
needle_cline.bsequence = filename2
needle_cline.gapopen = int(gapopen)
needle_cline.gapextend = int(gapextend)
needle_cline.outfile = "needle.txt"
print(needle_cline)
print(needle_cline.outfile)
stdout, stderr = needle_cline()
print(stdout + stderr)
align = AlignIO.read("needle.txt", "emboss")
file = open("needle.txt", "r")
# print(file.read())
view = ("\n\n%s" % file.read())
root = Tk()
S = Scrollbar(root)
T = Text(root, height=4, width=500)
S.pack(side=RIGHT, fill=Y)
T.pack(side=LEFT, fill=Y)
S.config(command=T.yview)
T.config(yscrollcommand=S.set)
T.insert(END, view)
with open(outfile, "w") as f:
f.write(view)
def GetExec(self, optList, frame):
# Respond to the "embossn" type command.
self.frame = frame
plugin_exe = r"C:/mEMBOSS/water.exe"
self.outfile = r"C:\Users\francis\Documents\Monguis\BioGui\plugins\water.txt"
self.outtype = "fasta"
cline = WaterCommandline(
plugin_exe,
asequence=str(self.frame.paramBoxes[1].GetValue()),
bsequence=str(self.frame.paramBoxes[3].GetValue()))
cline.outfile = self.outfile
cline.gapopen = self.param[7].GetValue()
cline.gapextend = self.param[9].GetValue()
if self.param[10].GetValue():
cline.similarity = True
else:
cline.similarity = False
if self.frame.abet == "AA":
cline.snucleotide = True
cline.sprotein = False
elif self.frame.abet == "DNA" or self.frame.abet == "RNA":
cline.snucleotide = True
cline.sprotein = False
if self.frame.options:
t = self.boxList[3].GetValue()
if t != '':
cline.datafile = str(t)
return str(cline)
def waterAlign(seq1, seq2, gapopen, gapextend):
water = WaterCommandline()
water.asequence = seq1
water.bsequence = seq2
water.gapopen = gapopen
water.gapextend = gapextend
water.outfile = "needle.txt"
stdout, stderr = water()
print(stdout)
def doWater(contig, seq):
with open("contig.faa", "w") as stuff1:
stuff1.write(">contig\n")
stuff1.write(contig)
with open("seq.faa", "w") as stuff2:
stuff2.write(">seq\n")
stuff2.write(str(seq))
water_cline = WaterCommandline()
water_cline.asequence="contig.faa"
water_cline.bsequence="seq.faa"
water_cline.gapopen=10
water_cline.gapextend=0.5
water_cline.outfile="water.txt"
stdout, stderr = water_cline()
print(stdout + stderr)
values = getStartEnd()
return values
def emboss_local_pairwise_alignment(query_dir, seq_type):
if seq_type == 'fg':
print '\n ...pairwise comparison of functional gene sequences...\n'
elif seq_type == 'ssu':
print '\n ...pairwise comparison of SSU rRNA sequences...\n'
water_cline = WaterCommandline()
water_cline.gapopen=10
water_cline.gapextend=0.5
query_list = [query for query in sorted(glob.glob(query_dir+"/*.fa"))]
for i, a_seq in enumerate(query_list):
water_cline.asequence=str(a_seq)
for j, b_seq in enumerate(query_list[i:]):
water_cline.bsequence=str(b_seq)
align_out = query_dir+"/pairwise_"+str(i+1)+"_"+str(i+j+1)+".aln"
water_cline.outfile=str(align_out)
water_cline()
print 'Done\n'
return query_dir+"/*.aln"
# http://rosalind.info/problems/swat/
from Bio.Emboss.Applications import WaterCommandline
from Bio import ExPASy, SeqIO
if __name__ == "__main__":
ids = open('rosalind_swat.txt').read().split(' ')
for i in ids:
handle = ExPASy.get_sprot_raw(i)
r = SeqIO.read(handle, "swiss")
handle.close()
with open(i, 'w') as f:
SeqIO.write(r, f, 'fasta')
water_cline = WaterCommandline()
water_cline.asequence = ids[0]
water_cline.bsequence = ids[1]
water_cline.outfile = "rosalind_swat_output.txt"
water_cline.gapopen = 10
water_cline.gapextend = 1
water_cline()
for line in open('rosalind_swat_output.txt').readlines():
if 'Score:' in line:
print(int(float(line[:-1].split(':')[-1].strip())))
# http://rosalind.info/problems/swat/
from Bio.Emboss.Applications import WaterCommandline
from Bio import ExPASy, SeqIO
if __name__ == "__main__":
ids = open('rosalind_swat.txt').read().split(' ')
for i in ids:
handle = ExPASy.get_sprot_raw(i)
r = SeqIO.read(handle, "swiss")
handle.close()
with open(i, 'w') as f:
SeqIO.write(r, f, 'fasta')
water_cline = WaterCommandline()
water_cline.asequence = ids[0]
water_cline.bsequence = ids[1]
water_cline.outfile = "rosalind_swat_output.txt"
water_cline.gapopen = 10
water_cline.gapextend = 1
water_cline()
for line in open('rosalind_swat_output.txt').readlines():
if 'Score:' in line:
print(int(float(line[:-1].split(':')[-1].strip())))