def main(argv=None):
"""script main.
parses command line options in sys.argv, unless *argv* is given.
"""
if argv is None:
argv = sys.argv
parser = E.OptionParser(
version=
"%prog version: $Id: malis2profiles.py 2782 2009-09-10 11:40:29Z andreas $",
usage=globals()["__doc__"])
parser.set_defaults()
(options, args) = E.Start(parser)
mali = Mali.SequenceCollection()
last_id = None
ninput, noutput, nskipped = 0, 0, 0
for line in sys.stdin:
if line[0] == "#":
continue
start, ali, end, id = line[:-1].split("\t")
ninput += 1
if id != last_id:
if last_id:
mali.setName(last_id)
mali.writeToFile(sys.stdout, format="profile")
noutput += 1
mali = Mali.SequenceCollection()
last_id = id
mali.addSequence(id, start, end, ali)
if last_id:
mali.setName(last_id)
mali.writeToFile(sys.stdout, format="profile")
noutput += 1
if options.loglevel >= 1:
options.stdlog.write("# ninput=%i, noutput=%i, nskipped=%i.\n" %
(ninput, noutput, nskipped))
E.Stop()
xrate_min_increment=0.000001,
with_rho=True,
separator="|",
single_omega=False,
shared_frequencies=False,
shared_rates=False,
block_size=None,
replicates=None,
)
(options, args) = Experiment.Start(parser)
if options.replicates != None:
# read a sequence collection with possible duplicate names
# used for benchmarking
mali = Mali.SequenceCollection()
else:
mali = Mali.Mali()
mali.readFromFile(sys.stdin, format=options.input_format)
options.stdout.write(
"seq1\tseq2\tdN\tdS\tdNdS\tN\tS\tdN_err\tdS_err\tkappa\tlnL\ttau\tlen")
if options.with_rho:
options.stdout.write("\trN\trS\tt\trN0\trS0\tt0")
options.stdout.write("\terror_str\n")
if options.replicates != None:
ids = mali.getIdentifiers()
def main(argv=None):
"""script main.
parses command line options in sys.argv, unless *argv* is given.
"""
if argv == None: argv = sys.argv
parser = E.OptionParser(
version=
"%prog version: $Id: mali2kaks.py 2781 2009-09-10 11:33:14Z andreas $")
parser.add_option("--set-omega",
dest="omega",
type="float",
help="initial omega value.")
parser.add_option("--set-kappa",
dest="kappa",
type="float",
help="initial kappa value.")
parser.add_option("--fix-kappa",
dest="fix_kappa",
action="store_true",
help="do not estimate kappa.")
parser.add_option("--fix-omega",
dest="fix_omega",
action="store_true",
help="do not estimate omega.")
parser.add_option("--set-codon-frequencies",
dest="codon_frequencies",
type="choice",
choices=("uniform", "fequal", "f3x4", "f1x4", "f61"),
help="set codon frequencies.")
parser.add_option("--set-method",
dest="paml_method",
type="int",
help="set paml optimization method [0|1].")
parser.add_option("--set-sequence-type",
dest="seqtype",
type="choice",
choices=("codon", "aa", "trans"),
help="sequence type.")
parser.add_option(
"--set-clean-data",
dest="clean_data",
type="choice",
choices=("0", "1"),
help=
"PAML should cleanup data: 0=only gaps within pair are removed, 1=columns in the mali with gaps are removed."
)
parser.add_option("--dump",
dest="dump",
action="store_true",
help="dump raw output [%default].")
parser.add_option("--set-optimization-threshold",
dest="optimization_threshold",
type="string",
help="set paml optimization threshold [%default].")
parser.add_option("-i",
"--input-format",
dest="input_format",
type="choice",
choices=("plain", "fasta", "clustal", "stockholm",
"phylip"),
help="input format of multiple alignment [%default].")
parser.add_option("--pairwise",
dest="pairwise",
action="store_true",
help="force pairwise comparison [%default].")
parser.add_option("--iteration",
dest="iteration",
type="choice",
choices=("all-vs-all", "first-vs-all", "pairwise",
"tree"),
help="iteration mode [%default].")
parser.add_option(
"--no-clean",
dest="clean_mali",
action="store_false",
help=
"do not clean multiple alignment before submitting to codeml. It might take too long for very large sequences."
)
parser.add_option("--method",
dest="method",
type="choice",
choices=("paml", "xrate"),
help="choose method for rate computation [%default]")
parser.add_option("--xrate-model",
dest="xrate_model",
type="choice",
choices=("f3x4-two", "f3x4-four", "sn", "akaksgc",
"ef3x4-four", "f3x4-fourproducts"),
help="models to use [%default].")
parser.add_option("-w",
"--write",
dest="write",
type="choice",
action="append",
choices=("input_fixed", "trained_fixed",
"input_variable", "trained_variable", "all"),
help="output sections to write [%default].")
parser.add_option("-o",
"--output-pattern",
dest="output_pattern",
type="string",
help="output pattern for output files [%default].")
parser.add_option("--xrate-insert-frequencies",
dest="xrate_insert_frequencies",
action="store_true",
help="estimate codon frequencies from input [%default].")
parser.add_option("--xrate-uniform-frequencies",
dest="xrate_insert_frequencies",
action="store_false",
help="use uniform codon frequencies [%default].")
parser.add_option("--xrate-fix-frequencies",
dest="xrate_fix_frequencies",
action="store_true",
help="set initial frequencies to const [%default].")
parser.add_option("--xrate-estimate-frequencies",
dest="xrate_fix_frequencies",
action="store_false",
help="estimate nucleotide frequencies [%default].")
parser.add_option(
"--xrate-fix-rates",
dest="fix_rates",
type="string",
help=
"""fix rates to specified values. Note that the number of rates has to match the ones
in the model. Provide values in a comma-separated list [%default].""")
parser.add_option(
"--xrate-min-increment",
dest="xrate_min_increment",
type=float,
help="minimum increment to stop iteration in xrate [%default].")
parser.add_option(
"--min-overlap",
dest="min_overlap",
type="int",
help="minimum overlap between a sequence pair in residues [%default].")
parser.add_option(
"--with-rho",
dest="with_rho",
action="store_true",
help=
"output rho values (substitution rates per codon). This requires a patched version of PAML [%default]."
)
parser.add_option(
"--with-counts",
dest="with_counts",
action="store_true",
help=
"output counts of aligned positions, transitions and transversions [%default]."
)
parser.add_option("--remove-stops",
dest="remove_stops",
action="store_true",
help="remove stop codons [%default].")
parser.add_option(
"--replicates",
dest="replicates",
type="int",
help="in benchmarking mode expect ## replicates [%default].")
parser.add_option("--tree",
dest="tree",
type="string",
help="use tree for estimation [%default].")
parser.set_defaults(
input_format="fasta",
omega=None,
codon_frequencies=None,
paml_method=None,
optimization_threshold=None,
seqtype="codon",
dump=False,
clean_data=False,
min_overlap=60,
gap_chars="-.",
mask_chars="nN",
pairwise=False,
kappa=None,
fix_kappa=False,
fix_omega=False,
clean_mali=True,
method="paml",
report_step=1000,
loglevel=1,
xrate_insert_frequencies=False,
xrate_fix_frequencies=False,
write=[],
output_pattern="%s.eg",
value_format="%6.4f",
fix_rates=None,
xrate_from_parameters=False,
xrate_model="f3x4-four",
with_rho=False,
with_counts=False,
iteration="all-vs-all",
remove_stops=False,
xrate_min_increment=0.000001,
replicates=None,
tree=None,
)
(options, args) = E.Start(parser)
if options.method == "xrate":
# imports for xrate computation
from XGram.Generator.Prebuilt import Codons
from XGram.Model import Annotation
import XGram.Run
import Bio.Data.CodonTable
# paml like estimation using xrate
if options.codon_frequencies == "uniform":
options.xrate_fix_frequencies = True
options.xrate_insert_frequencies = False
elif options.codon_frequencies == "f3x4":
options.xrate_fix_frequencies = True
options.xrate_insert_frequencies = True
elif options.method == "paml":
if not options.codon_frequencies:
options.codon_frequencies = "F3X4"
if options.fix_rates:
options.fix_rates = map(float, options.fix_rates.split(","))
if options.pairwise or options.replicates:
## read sequences, but not as a multiple alignment. This permits multiple names.
mali = Mali.SequenceCollection()
else:
mali = Mali.Mali()
mali.readFromFile(sys.stdin, format=options.input_format)
E.info("read multiple alignment")
if mali.getLength() == 0:
raise "refusing to process empty alignment."
################################################################
################################################################
################################################################
## setup methods
################################################################
options.stdout.write(
"seq1\tseq2\tdN\tdS\tdNdS\tN\tS\tdN_err\tdS_err\tkappa\tlnL\ttau")
if options.with_rho:
options.stdout.write("\trN\trS\tt\trN0\trS0\tt0")
if options.with_counts:
options.stdout.write("\t%s" % Genomics.SequencePairInfo().getHeader())
options.stdout.write("\terror_str\n")
if options.replicates != None:
ids = mali.getIdentifiers()
assert (len(ids) % options.replicates == 0)
s = len(ids) / options.replicates
for x in range(0, len(ids), s):
m = Mali.Mali()
for id in ids[x:x + s]:
m.addEntry(mali.getEntry(id))
processMali(m, options)
else:
processMali(mali, options)
E.Stop()
def main(argv=sys.argv):
parser = E.OptionParser(
version=
"%prog version: $Id: mali2mali.py 2782 2009-09-10 11:40:29Z andreas $",
usage=globals()["__doc__"])
parser.add_option(
"-i",
"--input-format",
dest="input_format",
type="choice",
choices=("plain", "fasta", "clustal", "stockholm", "phylip"),
help="input format of multiple alignment [default=%default].")
parser.add_option(
"-o",
"--output-format",
dest="output_format",
type="choice",
choices=("plain", "fasta", "stockholm", "phylip", "nexus",
"plain-fasta"),
help="output format of multiple alignment [default=%default].")
parser.add_option(
"--with-ranges",
dest="with_ranges",
action="store_true",
help=
"output alignment ranges (suffix /from-to after identifier) [default=%default]."
)
parser.add_option(
"--without-ranges",
dest="with_ranges",
action="store_false",
help=
"do not output alignment ranges (suffix /from-to after identifier) [default=%default]."
)
parser.add_option("-u",
"--allow-duplicates",
dest="allow_duplicates",
action="store_true",
help="permit duplicate entries [default=%default].")
parser.add_option(
"-m",
"--method",
dest="methods",
type="string",
help=
"""methods to apply. Several methods can be specified in a ','-separated list [default=%default]."""
)
parser.add_option(
"-p",
"--parameters",
dest="parameters",
type="string",
help="parameter stack for methods that require one [default=%default]."
)
parser.add_option(
"-a",
"--mask-char",
dest="mask_char",
type="string",
help="character to identify/set masked characters [default=%default].")
parser.set_defaults(
input_format="fasta",
output_format="fasta",
methods="",
parameters="",
mask_char="x",
gap_chars="-.nN",
with_ranges=True,
allow_duplicates=False,
)
(options, args) = E.Start(parser)
options.methods = options.methods.split(",")
options.parameters = options.parameters.split(",")
# 1. read multiple alignment in various formats
if options.allow_duplicates:
mali = Mali.SequenceCollection()
else:
mali = Mali.Mali()
t1 = time.time()
mali.readFromFile(options.stdin, format=options.input_format)
E.info("read mali with %i entries in %i seconds." %
(len(mali), time.time() - t1))
if len(mali) == 0:
raise ValueError("empty multiple alignment")
for method in options.methods:
t1 = time.time()
if method == "remove-unaligned-ends":
mali.removeUnalignedEnds()
elif method == "remove-end-gaps":
mali.removeEndGaps()
elif method == "remove-all-gaps":
mali.removeGaps(minimum_gaps=len(mali))
elif method == "remove-any-gaps":
mali.removeGaps(minimum_gaps=1)
elif method == "remove-some-gaps":
minimum_gaps = int(options.parameters[0])
del options.parameters[0]
mali.removeGaps(minimum_gaps=minimum_gaps)
elif method == "remove-empty-sequences":
mali.removeEmptySequences()
elif method == "upper":
mali.upperCase()
elif method == "lower":
mali.lowerCase()
elif method == "mark-codons":
mali.markCodons()
elif method == "remove-stops":
mali.removePattern(lambda x: x.upper() in ("TAG", "TAA", "TGA"),
allowed_matches=0,
minimum_matches=1,
delete_frame=3,
search_frame=3)
elif method == "shift-alignment":
map_id2offset = IOTools.ReadMap(open(options.parameters[0], "r"),
map_functions=(str, int))
del options.parameters[0]
mali.shiftAlignment(map_id2offset)
elif method == "propagate-masks":
mali.propagateMasks(mask_char=options.mask_char)
elif method == "recount":
mali.recount()
elif method in ("mark-transitions", "filter-odd-transitions",
"filter-even-transitions", "keep-even-segments",
"keep-odd-segments"):
if os.path.exists(options.parameters[0]):
map_id2transitions = IOTools.readMultiMap(
open(options.parameters[0], "r"), map_functions=(str, int))
else:
map_id2transitions = {}
r = map(int, options.parameters[0].split(':'))
r.sort()
map_id2transitions["mali"] = r
del options.parameters[0]
if method == "mark-transitions":
mali.markTransitions(map_id2transitions)
elif method in ("filter-odd-transitions", "keep-even-segments"):
mali.markTransitions(map_id2transitions, mode="keep-odd")
elif method in ("filter-even-transitions", "keep-odd-segments"):
mali.markTransitions(map_id2transitions, mode="keep-even")
elif method == "propagate-transitions":
mali.propagateTransitions()
elif method == "map-annotation":
# map annotations in one mali (stockholm-format) to the annotations in another.
# Note: the first two sequence identifiers must be shared and the sequence of the
# same length
other_mali = Mali.Mali()
other_mali.readFromFile(open(options.parameters[0], "r"),
format="stockholm")
del options.parameters[0]
mali.copyAnnotations(other_mali)
elif method == "add-annotation":
annotation_type, annotation_file = options.parameters[:2]
del options.parameters[:2]
AddAnnotation(mali, annotation_type, annotation_file)
elif method == "mask-columns":
annotation_type, annotation_file = options.parameters[:2]
del options.parameters[:2]
maskColumns(mali, annotation_type, annotation_file)
elif method == "remove-unaligned-pairs":
removeUnalignedPairs(mali, options)
elif method == "filter-3rd":
filterMali(mali, "3rd")
elif method == "filter-4d":
filterMali(mali, "4d")
elif method in ("mask-seg", "mask-bias"):
a, b = method.split("-")
maskMali(mali, b)
elif method == "exclude-with-stop":
mali.filter(method="with-stop")
elif method == "exclude-with-stop":
mali.filter(method="with-frameshift")
E.info("applied method %s in %i seconds." % (method, time.time() - t1))
mali.writeToFile(options.stdout,
format=options.output_format,
write_ranges=options.with_ranges)
E.Stop()
def main(argv=None):
"""script main.
parses command line options in sys.argv, unless *argv* is given.
"""
if argv is None:
argv = sys.argv
parser = E.OptionParser(
version=
"%prog version: $Id: mali2rates.py 2781 2009-09-10 11:33:14Z andreas $",
usage=globals()["__doc__"])
parser.add_option("-i",
"--input-format",
dest="input_format",
type="choice",
choices=("plain", "fasta", "clustal", "stockholm",
"phylip"),
help="input format of multiple alignment")
parser.add_option(
"-s",
"--sites",
dest="sites",
type="string",
help="sites to use [default=%default].",
)
parser.add_option(
"-f",
"--file",
dest="filename",
type="string",
help="filename of multiple alignment (- for stdin) [default=%default].",
metavar="FILE")
parser.add_option("-o",
"--format",
dest="format",
type="string",
help="format [default=%default].",
metavar="format")
parser.add_option(
"-d",
"--distance",
dest="distance",
type="choice",
choices=("PID", "T92", "JC69", "POVL", "F84", "LogDet", "K80", "F81",
"HKY85", "TN93", "REV", "UNREST", "REVU", "UNRESTU", "JTT",
"PMB", "PAM", "Kimura", "CategoriesModel"),
help="method to use for distance calculation [default=%default].")
parser.add_option("--method",
dest="method",
type="choice",
choices=("phylip", "baseml", "own", "xrate"),
help="program to use for rate calculation.")
parser.add_option("--output-format",
dest="output_format",
type="choice",
choices=("list", "tree"),
help="output format.")
parser.add_option(
"-m",
"--min-sites",
dest="min_sites",
type="int",
help="minimum number of sites for output[default=%default].",
)
parser.add_option(
"-a",
"--alphabet",
dest="alphabet",
type="choice",
choices=("aa", "na", "auto"),
help="alphabet to use.",
)
parser.add_option("-t",
"--filename-tree",
dest="filename_tree",
type="string",
help="filename with tree information.")
parser.add_option("--set-alpha",
dest="alpha",
type="float",
help="initial alpha value.")
parser.add_option("--fix-alpha",
dest="fix_alpha",
action="store_true",
help="do not estimate alpha.")
parser.add_option("--set-kappa",
dest="kappa",
type="float",
help="initial kappa value.")
parser.add_option("--fix-kappa",
dest="fix_kappa",
action="store_true",
help="do not estimate kappa.")
parser.add_option("--dump",
dest="dump",
action="store_true",
help="dump output.")
parser.add_option("--test",
dest="test",
action="store_true",
help="test run - does not clean up.")
parser.add_option("--pairwise",
dest="pairwise",
action="store_true",
help="force pairwise comparison.")
parser.add_option(
"--set-clean-data",
dest="clean_data",
type="choice",
choices=("0", "1"),
help=
"PAML should cleanup data: 0=only gaps within pair are removed, 1=columns in the mali with gaps are removed."
)
parser.add_option(
"--with-counts",
dest="with_counts",
action="store_true",
help=
"output counts of aligned positions, transitions and transversions.")
parser.add_option("-w",
"--write",
dest="write",
type="choice",
action="append",
choices=("input", "trained", "all"),
help="output sections to write for xrate.")
parser.add_option("--output-pattern",
dest="output_pattern",
type="string",
help="output pattern for output files.")
parser.add_option("--xrate-min-increment",
dest="xrate_min_increment",
type=float,
help="minimum increment to stop iteration in xrate.")
parser.set_defaults(
input_format="fasta",
filename_tree=None,
with_counts=False,
sites="d4",
distance="T92",
min_sites=1,
filename="-",
alphabet="auto",
format="%6.4f",
method="phylip",
kappa=None,
fix_kappa=False,
alpha=None,
fix_alpha=False,
dump=False,
clean_data=None,
output_format="list",
iteration="all-vs-all",
pairwise=False,
report_step=1000,
output_pattern="%s.eg",
write=[],
test_xrate=False,
xrate_min_increment=None,
is_codons=False,
)
(options, args) = E.Start(parser)
if options.filename != "-":
infile = open(options.filename, "r")
else:
infile = sys.stdin
# read multiple alignment
if options.pairwise:
# read sequences, but not as a multiple alignment. This permits
# multiple names.
mali = Mali.SequenceCollection()
options.iteration = "pairwise"
else:
mali = Mali.Mali()
mali.readFromFile(infile, format=options.input_format)
ids = mali.getIdentifiers()
if options.alphabet == "auto":
s = "".join(map(lambda x: x.mString, mali.values())).lower()
ss = re.sub("[acgtxn]", "", s)
if float(len(ss)) < (len(s) * 0.1):
options.alphabet = "na"
if mali.getNumColumns() % 3 == 0:
options.is_codons = True
else:
options.alphabet = "aa"
if options.loglevel >= 1:
options.stdlog.write("# autodetected alphabet: %s\n" %
options.alphabet)
if options.filename != "-":
infile.close()
npairs = 0
nskipped_length = 0
nskipped_distance = 0
pairs = []
if options.iteration == "all-vs-all":
for x in range(len(ids) - 1):
for y in range(x + 1, len(ids)):
pairs.append((x, y))
elif options.iteration == "first-vs-all":
for y in range(1, len(ids)):
pairs.append((0, y))
elif options.iteration == "pairwise":
if len(ids) % 2 != 0:
raise "uneven number of sequences (%i) not compatible with --iteration=pairwise" % len(
ids)
for x in range(0, len(ids), 2):
pairs.append((x, x + 1))
if options.alphabet == "na":
if options.method == "baseml":
runBaseML(mali, pairs, options)
elif options.method == "phylip" and options.distance in ("F84", "K80",
"JC69",
"LogDet"):
runDNADIST(mali, pairs, options)
elif options.method == "xrate":
runXrate(mali, pairs, options)
else:
if options.is_codons:
h = Genomics.SequencePairInfoCodons().getHeader()
else:
h = Genomics.SequencePairInfo().getHeader()
options.stdout.write("seq1\tseq2\tdist\tvar\t%s\n" % (h))
for x, y in pairs:
id_x = ids[x]
npairs += 1
id_y = ids[y]
info = Genomics.CalculatePairIndices(
mali[id_x], mali[id_y], with_codons=options.is_codons)
if options.distance in ("T92", "JC69"):
if options.sites == "d4":
seq1, seq2 = Genomics.GetDegenerateSites(mali[id_x],
mali[id_y],
position=3,
degeneracy=4)
if len(seq1) < options.min_sites:
nskipped_length += 1
continue
else:
raise "unknown sites %s" % options.sites
if options.distance == "T92":
distance, variance = CalculateDistanceT92(info)
elif options.distance == "JC69":
distance, variance = CalculateDistanceJC69(info)
elif options.distance == "PID":
distance, variance = CalculateDistancePID(
mali[id_x], mali[id_y])
elif options.distance == "POVL":
distance, variance = CalculateDistancePOVL(
mali[id_x], mali[id_y])
if distance >= 0:
options.stdout.write("\t".join(
map(str, (id_x, id_y, options.format % distance,
options.format % variance, info))) + "\n")
else:
nskipped_distance += 1
elif options.alphabet == "aa":
if options.distance in ("JTT", "PMB", "PAM", "Kimura",
"CategoriesModel"):
# use phylip for these
phylip = WrapperPhylip.Phylip()
phylip.setProgram("protdist")
phylip.setMali(mali)
phylip_options = []
if options.distance == "PMG":
phylip_options += ["D"] * 1
elif options.distance == "PAM":
phylip_options += ["D"] * 2
elif options.distance == "Kimura":
phylip_options += ["D"] * 3
elif options.distance == "CategoriesModel":
phylip_options += ["D"] * 4
phylip_options.append("Y")
phylip.setOptions(phylip_options)
result = phylip.run()
writePhylipResult(result, options)
else:
options.stdout.write("id1\tid2\tdist\tvar\n")
# iterate over all pairs of sequences
for x, y in pairs:
id_x = ids[x]
npairs += 1
id_y = ids[y]
if options.distance == "PID":
distance, variance = CalculateDistancePID(
mali[id_x], mali[id_y])
elif options.distance == "POVL":
# percentage overlap
distance, variance = CalculateDistancePOVL(
mali[id_x], mali[id_y])
if distance >= 0:
options.stdout.write("\t".join(
(id_x, id_y, options.format % distance,
options.format % variance)) + "\n")
else:
nskipped_distance += 1
if options.loglevel >= 1:
options.stdlog.write(
"# nseqs=%i, npairs=%i, nskipped_length=%i, nskipped_distance=%i\n"
% (len(ids), npairs, nskipped_length, nskipped_distance))
E.Stop()
def main(argv=sys.argv):
parser = E.OptionParser(version="%prog version: $Id$",
usage=globals()["__doc__"])
parser.add_option("-i",
"--input-format",
dest="input_format",
type="choice",
choices=("plain", "fasta", "clustal", "stockholm"),
help="input format of multiple alignment")
parser.add_option(
"-a",
"--alphabet",
dest="alphabet",
type="choice",
choices=("aa", "na"),
help="alphabet to use [default=%default].",
)
parser.add_option("-s",
"--sections",
dest="sections",
type="choice",
action="append",
choices=("length", "composition", "entropy", "all"),
help="which sections to output")
parser.add_option("-u",
"--allow-duplicates",
dest="allow_duplicates",
action="store_true",
help="permit duplicate entries [default=%default].")
parser.set_defaults(
input_format="fasta",
output_format="fasta",
mask_chars="nN",
gap_chars="-.",
alphabet="na",
sections=[],
allow_duplicates=False,
)
(options, args) = E.Start(parser)
if len(options.sections) == 0:
raise ValueError("please supply at least one method.")
if "all" in options.sections:
options.sections = ["length", "composition", "entropy"]
counters = []
def getCounter(section):
if options.alphabet == "na":
if section == "length":
s = SequencePropertiesLength()
elif section == "composition":
s = SequencePropertiesNA()
elif section == "entropy":
s = SequencePropertiesEntropy("ACGT")
else:
raise ValueError("unknown section %s" % section)
elif options.alphabet == "aa":
if section == "length":
s = SequencePropertiesLength()
elif section == "composition":
s = SequencePropertiesAminoAcids()
elif section == "entropy":
s = SequencePropertiesEntropy("ACDEFGHIKLMNPQRSTVWY")
else:
raise ValueError("unknown section %s" % section)
return s
# read multiple alignment in various formats
# 1. read multiple alignment in various formats
if options.allow_duplicates:
mali = Mali.SequenceCollection()
else:
mali = Mali.Mali()
mali.readFromFile(options.stdin, format=options.input_format)
# do not use column, as it is a reserved word in sql
options.stdout.write("col")
for section in options.sections:
options.stdout.write("\t" +
"\t".join(getCounter(section).getHeaders()))
options.stdout.write("\n")
columns = mali.getColumns()
counter = E.Counter()
for x, column in enumerate(columns):
counter.input += 1
sequence = "".join(column)
options.stdout.write("%i" % x)
for section in options.sections:
s = getCounter(section)
s.loadSequence(sequence)
options.stdout.write("\t" + "\t".join(s.getFields()))
options.stdout.write("\n")
counter.output += 1
E.info("%s" % str(counter))
E.Stop()
