def __init__(self, seqTab, sequence, position, minusPrimer, plusPrimer, parent = None):
super(Sequence, self).__init__()
self.seqTab = seqTab
self.baseClassL = self.seqTab.baseClassL
self.sortBaseL = self.seqTab.sortBaseL
print 'Id from Sequence item: ' + str(id(self.baseClassL))
self.fill = QColor(100, 204, 150)
self.seq = sequence
self.rect = QRectF(0, 0, 12*len(self.seq), 30)
# self.setFlags(QGraphicsItem.ItemIsMovable)
self.minusPrimer = minusPrimer
self.plusPrimer = plusPrimer
count = 0
for nucleotide in self.seq:
if count >= (len(self.seq))/2:
primer = self.minusPrimer
else:
primer = self.plusPrimer
base = Base(nucleotide, primer)
base.setParentItem(self)
base.setPos(count*12, 0)
count = count + 1
self.setPos(position[0], position[1])
def hybridize(self):
count = 0
for nucleotide in self.seq:
base = Base(self.complement(nucleotide))
self.hybBases.append(base)
base.setParentItem(self)
base.setPos(count, -15)
count = count + 12
self.scene().removeItem(self.seqHybAll)
self.seqDenAll.setParentItem(self)
self.seqDenAll.setPos(12*len(self.seq)/2 - 12*len('Denature')/2, 28)
def __init__(self, mainwin, sequence, position, primerLetter, parent = None):
super(Primer, self).__init__()
self.fill = QColor(170, 54, 20)
self.seq = sequence
self.rect = QRectF(0, 0, 12*len(self.seq), 45)
self.setAcceptHoverEvents(True)
# self.setFlags(QGraphicsItem.ItemIsMovable)
self.primerLetter = primerLetter
count = 0
for nucleotide in self.seq:
base = Base(nucleotide, None)
base.setParentItem(self)
base.setPos(count, 0)
count = count + 12
self.isHybed = False
self.hybBases = []
self.plusLabel = QGraphicsSimpleTextItem(QString('Plus'))
self.minusLabel = QGraphicsSimpleTextItem(QString('Minus'))
self.primerLabel = QGraphicsSimpleTextItem(QString(self.primerLetter))
self.plusLabel.setParentItem(self)
self.minusLabel.setParentItem(self)
self.primerLabel.setParentItem(self)
self.minusLabel.setPos(0, 15)
self.plusLabel.setPos(12*len(self.seq)- self.plusLabel.boundingRect().width(), 15)
self.primerLabel.setPos(12*len(self.seq)/2, 28)
"""
self.seqDenAll = QGraphicsSimpleTextItem(QString('Denature'))
self.seqHybAll = QGraphicsSimpleTextItem(QString('Hybridize'))
self.seqHybAll.setParentItem(self)
self.seqHybAll.setPos(12*len(self.seq)/2 - 12*len('Hybridize')/2, 28)
"""
"""
psSeq = self.seq[len(self.seq)-6:]
self.primeSite = SeqFunc(QColor(130, 190, 50), psSeq, [12*len(self.seq)-12*len(psSeq), 15], self)
self.primeSite.setParentItem(self)
"""
self.setPos(position[0], position[1])
