def test_dont_load_file():
csv_text = """"Image_FileName_DNA","Image_PathName_DNA"
"%s","%s"
""" % (
test_filename,
test_path,
)
pipeline, module, filename = make_pipeline(csv_text)
c0_ran = [False]
def callback(workspace):
imgset = workspace.image_set
assert len(imgset.names) == 0
c0_ran[0] = True
c0 = C0()
c0.callback = callback
c0.set_module_num(1)
pipeline.add_module(c0)
try:
module.wants_images.value = False
pipeline.run()
assert c0_ran[0]
finally:
os.remove(filename)
def make_workspace(image, labels):
module = cellprofiler.modules.definegrid.DefineGrid()
module.set_module_num(1)
module.grid_image.value = GRID_NAME
module.manual_image.value = INPUT_IMAGE_NAME
module.display_image_name.value = INPUT_IMAGE_NAME
module.object_name.value = OBJECTS_NAME
module.save_image_name.value = OUTPUT_IMAGE_NAME
image_set_list = cellprofiler.image.ImageSetList()
image_set = image_set_list.get_image_set(0)
image_set.add(INPUT_IMAGE_NAME, cellprofiler.image.Image(image))
object_set = cellprofiler.object.ObjectSet()
objects = cellprofiler.object.Objects()
objects.segmented = labels
object_set.add_objects(objects, OBJECTS_NAME)
pipeline = cellprofiler.pipeline.Pipeline()
def callback(caller, event):
assert not isinstance(event, cellprofiler.pipeline.LoadExceptionEvent)
assert not isinstance(event, cellprofiler.pipeline.RunExceptionEvent)
pipeline.add_listener(callback)
pipeline.add_module(module)
measurements = cellprofiler.measurement.Measurements()
workspace = cellprofiler.workspace.Workspace(
pipeline, module, image_set, object_set, measurements, image_set_list
)
return workspace, module
def make_workspace(self,
image,
size_method,
interpolation,
mask=None,
cropping=None,
dimensions=2):
module = cellprofiler.modules.resize.Resize()
module.x_name.value = INPUT_IMAGE_NAME
module.y_name.value = OUTPUT_IMAGE_NAME
module.size_method.value = size_method
module.interpolation.value = interpolation
module.module_num = 1
pipeline = cellprofiler.pipeline.Pipeline()
pipeline.add_module(module)
image_set_list = cellprofiler.image.ImageSetList()
image_set = image_set_list.get_image_set(0)
image = cellprofiler.image.Image(image,
mask,
cropping,
dimensions=dimensions)
image_set.add(INPUT_IMAGE_NAME, image)
workspace = cellprofiler.workspace.Workspace(
pipeline, module, image_set, cellprofiler.object.ObjectSet(),
cellprofiler.measurement.Measurements(), image_set_list)
return workspace, module
def test_10_01_scaling(self):
'''Test loading an image scaled and unscaled'''
folder = "loaddata"
file_name = "1-162hrh2ax2.tif"
path = tests.modules.make_12_bit_image(folder, file_name, (22, 18))
csv_text = ("Image_PathName_MyFile,Image_FileName_MyFile\n"
"%s,%s\n" % os.path.split(path))
c0_image = []
for rescale in (False, True):
pipeline, module, filename = self.make_pipeline(csv_text)
try:
module.rescale.value = rescale
def callback(workspace):
imgset = workspace.image_set
image = imgset.get_image("MyFile")
pixels = image.pixel_data
c0_image.append(pixels.copy())
c0 = C0()
c0.callback = callback
c0.module_num = 2
pipeline.add_module(c0)
pipeline.run()
finally:
os.remove(filename)
unscaled, scaled = c0_image
numpy.testing.assert_almost_equal(unscaled * 65535. / 4095., scaled)
def test_10_01_scaling(self):
"""Test loading an image scaled and unscaled"""
folder = "loaddata"
file_name = "1-162hrh2ax2.tif"
path = tests.modules.make_12_bit_image(folder, file_name, (22, 18))
csv_text = (
"Image_PathName_MyFile,Image_FileName_MyFile\n"
"%s,%s\n" % os.path.split(path)
)
c0_image = []
for rescale in (False, True):
pipeline, module, filename = self.make_pipeline(csv_text)
try:
module.rescale.value = rescale
def callback(workspace):
imgset = workspace.image_set
image = imgset.get_image("MyFile")
pixels = image.pixel_data
c0_image.append(pixels.copy())
c0 = C0()
c0.callback = callback
c0.module_num = 2
pipeline.add_module(c0)
pipeline.run()
finally:
os.remove(filename)
unscaled, scaled = c0_image
numpy.testing.assert_almost_equal(unscaled * 65535.0 / 4095.0, scaled)
def make_workspace(gridding, labels=None):
module = cellprofiler.modules.identifyobjectsingrid.IdentifyObjectsInGrid()
module.set_module_num(1)
module.grid_name.value = GRID_NAME
module.output_objects_name.value = OUTPUT_OBJECTS_NAME
module.guiding_object_name.value = GUIDING_OBJECTS_NAME
image_set_list = cellprofiler.image.ImageSetList()
object_set = cellprofiler.object.ObjectSet()
if labels is not None:
my_objects = cellprofiler.object.Objects()
my_objects.segmented = labels
object_set.add_objects(my_objects, GUIDING_OBJECTS_NAME)
pipeline = cellprofiler.pipeline.Pipeline()
def callback(caller, event):
assert not isinstance(event, cellprofiler.pipeline.RunExceptionEvent)
pipeline.add_listener(callback)
pipeline.add_module(module)
workspace = cellprofiler.workspace.Workspace(
pipeline,
module,
image_set_list.get_image_set(0),
object_set,
cellprofiler.measurement.Measurements(),
image_set_list,
)
workspace.set_grid(GRID_NAME, gridding)
return workspace, module
def test_11_02_invert_binary_invert(self):
#
# Regression for issue #1329
#
r = numpy.random.RandomState()
r.seed(1102)
m = cellprofiler.measurement.Measurements()
pixel_data = r.uniform(size=(20, 20)) > .5
m.add("inputimage", cellprofiler.image.Image(pixel_data))
module = cellprofiler.modules.imagemath.ImageMath()
module.images[0].image_name.value = "inputimage"
module.output_image_name.value = "intermediateimage"
module.operation.value = cellprofiler.modules.imagemath.O_INVERT
module.module_num = 1
pipeline = cellprofiler.pipeline.Pipeline()
pipeline.add_module(module)
module = cellprofiler.modules.imagemath.ImageMath()
module.images[0].image_name.value = "intermediateimage"
module.output_image_name.value = "outputimage"
module.operation.value = cellprofiler.modules.imagemath.O_INVERT
module.module_num = 2
pipeline = cellprofiler.pipeline.Pipeline()
workspace = cellprofiler.workspace.Workspace(pipeline, module, m, None,
m, None)
for module in pipeline.modules():
module.run(workspace)
numpy.testing.assert_array_equal(
pixel_data,
m.get_image("inputimage").pixel_data > .5)
def make_workspace(file_names):
module = cellprofiler.modules.loadsingleimage.LoadSingleImage()
module.set_module_num(1)
module.directory.set_dir_choice(
cellprofiler.preferences.DEFAULT_INPUT_FOLDER_NAME)
for i, file_name in enumerate(file_names):
if i > 0:
module.add_file()
module.file_settings[i].image_name.value = get_image_name(i)
module.file_settings[i].file_name.value = file_name
pipeline = cellprofiler.pipeline.Pipeline()
def callback(caller, event):
assert not isinstance(event,
cellprofiler.pipeline.RunExceptionEvent)
pipeline.add_listener(callback)
pipeline.add_module(module)
image_set_list = cellprofiler.image.ImageSetList()
workspace = cellprofiler.workspace.Workspace(
pipeline,
module,
image_set_list.get_image_set(0),
cellprofiler.object.ObjectSet(),
cellprofiler.measurement.Measurements(),
image_set_list,
)
return workspace, module
def test_01_01_combine():
img = get_my_image()
inj = cellprofiler.modules.injectimage.InjectImage("my_image", img)
inj.module_num = 1
ctg = cellprofiler.modules.colortogray.ColorToGray()
ctg.module_num = 2
ctg.image_name.value = "my_image"
ctg.combine_or_split.value = cellprofiler.modules.colortogray.COMBINE
ctg.red_contribution.value = 1
ctg.green_contribution.value = 2
ctg.blue_contribution.value = 3
ctg.grayscale_name.value = "my_grayscale"
pipeline = cellprofiler.pipeline.Pipeline()
pipeline.add_module(inj)
pipeline.add_module(ctg)
pipeline.test_valid()
measurements = cellprofiler.measurement.Measurements()
object_set = cellprofiler.object.ObjectSet()
image_set_list = cellprofiler.image.ImageSetList()
workspace = Workspace(pipeline, inj, None, None, measurements,
image_set_list, None)
inj.prepare_run(workspace)
inj.prepare_group(workspace, {}, [1])
image_set = image_set_list.get_image_set(0)
inj.run(Workspace(pipeline, inj, image_set, object_set, measurements, None))
ctg.run(Workspace(pipeline, ctg, image_set, object_set, measurements, None))
grayscale = image_set.get_image("my_grayscale")
assert grayscale
img = grayscale.image
numpy.testing.assert_almost_equal(img[0, 0], 1.0 / 6.0)
numpy.testing.assert_almost_equal(img[0, 25], 1.0 / 3.0)
numpy.testing.assert_almost_equal(img[25, 0], 1.0 / 2.0)
numpy.testing.assert_almost_equal(img[25, 25], 0)
def make_workspace(pixel_data, mask=None):
image = cellprofiler.image.Image(pixel_data, mask)
image_set_list = cellprofiler.image.ImageSetList()
image_set = image_set_list.get_image_set(0)
image_set.add(IMAGE_NAME, image)
module = cellprofiler.modules.identifydeadworms.IdentifyDeadWorms()
module.set_module_num(1)
module.image_name.value = IMAGE_NAME
module.object_name.value = OBJECTS_NAME
pipeline = cellprofiler.pipeline.Pipeline()
def callback(caller, event):
assert not isinstance(event, cellprofiler.pipeline.LoadExceptionEvent)
assert not isinstance(event, cellprofiler.pipeline.RunExceptionEvent)
pipeline.add_listener(callback)
pipeline.add_module(module)
workspace = cellprofiler.workspace.Workspace(
pipeline,
module,
image_set,
cellprofiler.object.ObjectSet(),
cellprofiler.measurement.Measurements(),
image_set_list,
)
return workspace, module
def test_03_01_well_row_column(self):
# Make sure that Metadata_Well is generated if we have
# Metadata_Row and Metadata_Column
#
for row_tag, column_tag in (("row", "column"), ("wellrow",
"wellcolumn"),
("well_row", "well_column")):
module = cellprofiler.modules.metadata.Metadata()
module.wants_metadata.value = True
em = module.extraction_methods[0]
em.filter_choice.value = cellprofiler.modules.metadata.F_ALL_IMAGES
em.extraction_method.value = cellprofiler.modules.metadata.X_MANUAL_EXTRACTION
em.source.value = cellprofiler.modules.metadata.XM_FILE_NAME
em.file_regexp.value = (
"^Channel(?P<Wavelength>[1-2])-"
"(?P<%(row_tag)s>[A-H])-"
"(?P<%(column_tag)s>[0-9]{2}).tif$") % locals()
em.filter_choice.value = cellprofiler.modules.metadata.F_ALL_IMAGES
url = "file:/imaging/analysis/Channel1-C-05.tif"
self.check(module, url, [{
"Wavelength": "1",
row_tag: "C",
column_tag: "05",
cellprofiler.measurement.FTR_WELL: "C05"
}])
pipeline = cellprofiler.pipeline.Pipeline()
imgs = cellprofiler.modules.images.Images()
imgs.filter_choice.value = cellprofiler.modules.images.FILTER_CHOICE_NONE
imgs.module_num = 1
pipeline.add_module(imgs)
module.module_num = 2
pipeline.add_module(module)
self.assertIn(
cellprofiler.measurement.M_WELL,
[c[1] for c in module.get_measurement_columns(pipeline)])
def test_04_02_dont_load_file(self):
csv_text = """"Image_FileName_DNA","Image_PathName_DNA"
"%s","%s"
""" % (
self.test_filename,
self.test_path,
)
pipeline, module, filename = self.make_pipeline(csv_text)
c0_ran = [False]
def callback(workspace):
imgset = workspace.image_set
self.assertEqual(len(imgset.names), 0)
c0_ran[0] = True
c0 = C0()
c0.callback = callback
c0.module_num = 1
pipeline.add_module(c0)
try:
module.wants_images.value = False
pipeline.run()
self.assertTrue(c0_ran[0])
finally:
os.remove(filename)
def test_04_01_load_objects(self):
r = numpy.random.RandomState()
r.seed(41)
labels = numpy.random.randint(0, 10, size=(30, 40))
filename = "myobjects.png"
directory = tempfile.mkdtemp()
cellprofiler.preferences.set_default_image_directory(directory)
pilimage = PIL.Image.fromarray(labels.astype(numpy.uint8), "L")
pilimage.save(os.path.join(directory, filename))
del pilimage
try:
module = cellprofiler.modules.loadsingleimage.LoadSingleImage()
module.module_num = 1
module.directory.set_dir_choice(
cellprofiler.modules.loadsingleimage.DEFAULT_INPUT_FOLDER_NAME)
fs = module.file_settings[0]
fs.file_name.value = filename
fs.image_objects_choice.value = cellprofiler.modules.loadsingleimage.IO_OBJECTS
fs.objects_name.value = OBJECTS_NAME
pipeline = cellprofiler.pipeline.Pipeline()
def callback(caller, event):
self.assertFalse(
isinstance(event, cellprofiler.pipeline.RunExceptionEvent))
pipeline.add_listener(callback)
pipeline.add_module(module)
m = cellprofiler.measurement.Measurements()
object_set = cellprofiler.object.ObjectSet()
image_set_list = cellprofiler.image.ImageSetList()
image_set = image_set_list.get_image_set(0)
workspace = cellprofiler.workspace.Workspace(
pipeline, module, image_set, object_set, m, image_set_list)
module.prepare_run(workspace)
module.run(workspace)
o = object_set.get_objects(OBJECTS_NAME)
numpy.testing.assert_equal(labels, o.segmented)
self.assertEqual(
m.get_current_image_measurement("_".join(
(cellprofiler.measurement.C_COUNT, OBJECTS_NAME))), 9)
self.assertEqual(
m.get_current_image_measurement("_".join(
(cellprofiler.modules.loadsingleimage.C_OBJECTS_FILE_NAME,
OBJECTS_NAME))), filename)
self.assertEqual(
m.get_current_image_measurement("_".join(
(cellprofiler.modules.loadsingleimage.C_OBJECTS_PATH_NAME,
OBJECTS_NAME))), directory)
for feature in (cellprofiler.measurement.M_LOCATION_CENTER_X,
cellprofiler.measurement.M_LOCATION_CENTER_Y,
cellprofiler.measurement.M_NUMBER_OBJECT_NUMBER):
values = m.get_current_measurement(OBJECTS_NAME, feature)
self.assertEqual(len(values), 9)
finally:
try:
os.remove(os.path.join(directory, filename))
os.rmdir(directory)
except:
print "Failed to delete directory " + directory
def test_04_01_extent(self):
module = cellprofiler.modules.measureobjectsizeshape.MeasureObjectAreaShape()
module.object_groups[0].name.value = "SomeObjects"
module.calculate_zernikes.value = True
object_set = cellprofiler.object.ObjectSet()
labels = numpy.zeros((10, 20), int)
# 3/4 of a square is covered
labels[5:7, 5:10] = 1
labels[7:9, 5:15] = 1
objects = cellprofiler.object.Objects()
objects.segmented = labels
object_set.add_objects(objects, "SomeObjects")
module.module_num = 1
image_set_list = cellprofiler.image.ImageSetList()
measurements = cellprofiler.measurement.Measurements()
pipeline = cellprofiler.pipeline.Pipeline()
pipeline.add_module(module)
def callback(caller, event):
self.assertFalse(isinstance(event, cellprofiler.pipeline.RunExceptionEvent))
pipeline.add_listener(callback)
workspace = cellprofiler.workspace.Workspace(pipeline, module,
image_set_list.get_image_set(0),
object_set, measurements, image_set_list)
module.run(workspace)
values = measurements.get_current_measurement(
"SomeObjects", "_".join((cellprofiler.modules.measureobjectsizeshape.AREA_SHAPE, cellprofiler.modules.measureobjectsizeshape.F_EXTENT)))
self.assertEqual(len(values), 1)
self.assertAlmostEqual(values[0], .75)
def test_03_01_well_row_column(self):
# Make sure that Metadata_Well is generated if we have
# Metadata_Row and Metadata_Column
#
for row_tag, column_tag in (
("row", "column"),
("wellrow", "wellcolumn"),
("well_row", "well_column")):
module = cellprofiler.modules.metadata.Metadata()
module.wants_metadata.value=True
em = module.extraction_methods[0]
em.filter_choice.value = cellprofiler.modules.metadata.F_ALL_IMAGES
em.extraction_method.value = cellprofiler.modules.metadata.X_MANUAL_EXTRACTION
em.source.value = cellprofiler.modules.metadata.XM_FILE_NAME
em.file_regexp.value = (
"^Channel(?P<Wavelength>[1-2])-"
"(?P<%(row_tag)s>[A-H])-"
"(?P<%(column_tag)s>[0-9]{2}).tif$") % locals()
em.filter_choice.value = cellprofiler.modules.metadata.F_ALL_IMAGES
url = "file:/imaging/analysis/Channel1-C-05.tif"
self.check(module, url,
[{ "Wavelength":"1",
row_tag:"C",
column_tag:"05",
cellprofiler.measurement.FTR_WELL: "C05"}])
pipeline = cellprofiler.pipeline.Pipeline()
imgs = cellprofiler.modules.images.Images()
imgs.filter_choice.value = cellprofiler.modules.images.FILTER_CHOICE_NONE
imgs.module_num = 1
pipeline.add_module(imgs)
module.module_num = 2
pipeline.add_module(module)
self.assertIn(
cellprofiler.measurement.M_WELL,
[c[1] for c in module.get_measurement_columns(pipeline)])
def test_01_00_zeros(self):
"""Run on an empty labels matrix"""
object_set = cellprofiler.object.ObjectSet()
labels = numpy.zeros((10, 20), int)
objects = cellprofiler.object.Objects()
objects.segmented = labels
object_set.add_objects(objects, "SomeObjects")
module = cellprofiler.modules.measureobjectsizeshape.MeasureObjectAreaShape()
settings = ["SomeObjects", "Yes"]
module.set_settings_from_values(settings, 1, module.module_class())
module.module_num = 1
image_set_list = cellprofiler.image.ImageSetList()
measurements = cellprofiler.measurement.Measurements()
pipeline = cellprofiler.pipeline.Pipeline()
pipeline.add_module(module)
workspace = cellprofiler.workspace.Workspace(pipeline, module,
image_set_list.get_image_set(0),
object_set, measurements, image_set_list)
module.run(workspace)
for f in (cellprofiler.modules.measureobjectsizeshape.F_AREA, cellprofiler.modules.measureobjectsizeshape.F_CENTER_X, cellprofiler.modules.measureobjectsizeshape.F_CENTER_Y,
cellprofiler.modules.measureobjectsizeshape.F_ECCENTRICITY, cellprofiler.modules.measureobjectsizeshape.F_EULER_NUMBER,
cellprofiler.modules.measureobjectsizeshape.F_EXTENT, cellprofiler.modules.measureobjectsizeshape.F_FORM_FACTOR,
cellprofiler.modules.measureobjectsizeshape.F_MAJOR_AXIS_LENGTH, cellprofiler.modules.measureobjectsizeshape.F_MINOR_AXIS_LENGTH,
cellprofiler.modules.measureobjectsizeshape.F_ORIENTATION, cellprofiler.modules.measureobjectsizeshape.F_PERIMETER,
cellprofiler.modules.measureobjectsizeshape.F_SOLIDITY, cellprofiler.modules.measureobjectsizeshape.F_COMPACTNESS,
cellprofiler.modules.measureobjectsizeshape.F_MAXIMUM_RADIUS, cellprofiler.modules.measureobjectsizeshape.F_MEAN_RADIUS,
cellprofiler.modules.measureobjectsizeshape.F_MEDIAN_RADIUS,
cellprofiler.modules.measureobjectsizeshape.F_MIN_FERET_DIAMETER, cellprofiler.modules.measureobjectsizeshape.F_MAX_FERET_DIAMETER):
m = cellprofiler.modules.measureobjectsizeshape.AREA_SHAPE + "_" + f
a = measurements.get_current_measurement('SomeObjects', m)
self.assertEqual(len(a), 0)
def make_workspace(self,
labels,
operation,
iterations=1,
wants_outlines=False,
wants_fill_holes=False):
object_set = cellprofiler.object.ObjectSet()
objects = cellprofiler.object.Objects()
objects.segmented = labels
object_set.add_objects(objects, INPUT_NAME)
module = cellprofiler.modules.expandorshrinkobjects.ExpandOrShrink()
module.object_name.value = INPUT_NAME
module.output_object_name.value = OUTPUT_NAME
module.outlines_name.value = OUTLINES_NAME
module.operation.value = operation
module.iterations.value = iterations
module.wants_outlines.value = wants_outlines
module.wants_fill_holes.value = wants_fill_holes
module.module_num = 1
pipeline = cellprofiler.pipeline.Pipeline()
pipeline.add_module(module)
image_set_list = cellprofiler.image.ImageSetList()
workspace = cellprofiler.workspace.Workspace(pipeline,
module,
image_set_list.get_image_set(0),
object_set,
cellprofiler.measurement.Measurements(),
image_set_list)
return workspace, module
def make_workspace(self, image, mask=None, labels=None, binary_image=None):
'''Make a workspace and IdentifyPrimaryObjects module
image - the intensity image for thresholding
mask - if present, the "don't analyze" mask of the intensity image
labels - if thresholding per-object, the labels matrix needed
binary_image - if thresholding using a binary image, the image
'''
module = YS.IdentifyYeastCells()
module.module_num = 1
module.input_image_name.value = IMAGE_NAME
module.object_name.value = OBJECTS_NAME
module.binary_image.value = BINARY_IMAGE_NAME
module.masking_objects.value = MASKING_OBJECTS_NAME
pipeline = cellprofiler.pipeline.Pipeline()
pipeline.add_module(module)
m = cpmeas.Measurements()
cpimage = cpi.Image(image, mask=mask)
m.add(IMAGE_NAME, cpimage)
if binary_image is not None:
m.add(BINARY_IMAGE_NAME, cpi.Image(binary_image))
object_set = cpo.ObjectSet()
if labels is not None:
o = cpo.Objects()
o.segmented = labels
object_set.add_objects(o, MASKING_OBJECTS_NAME)
workspace = cellprofiler.workspace.Workspace(pipeline, module, m,
object_set, m, None)
return workspace, module
def make_workspace(self, parents, children, fake_measurement=False):
"""Make a workspace for testing Relate"""
pipeline = cellprofiler.pipeline.Pipeline()
if fake_measurement:
class FakeModule(cellprofiler.module.Module):
def get_measurement_columns(self, pipeline):
return [
(
CHILD_OBJECTS,
MEASUREMENT,
cellprofiler.measurement.COLTYPE_FLOAT,
),
(
CHILD_OBJECTS,
IGNORED_MEASUREMENT,
cellprofiler.measurement.COLTYPE_INTEGER,
),
]
module = FakeModule()
module.module_num = 1
pipeline.add_module(module)
module = cellprofiler.modules.relateobjects.Relate()
module.x_name.value = PARENT_OBJECTS
module.y_name.value = CHILD_OBJECTS
module.find_parent_child_distances.value = (
cellprofiler.modules.relateobjects.D_NONE
)
module.wants_child_objects_saved.value = False
module.module_num = 2 if fake_measurement else 1
pipeline.add_module(module)
def make_tile_workspace(images):
module = cellprofiler.modules.tile.Tile()
module.set_module_num(1)
module.tile_method.value = cellprofiler.modules.tile.T_ACROSS_CYCLES
module.input_image.value = INPUT_IMAGE_NAME
module.output_image.value = OUTPUT_IMAGE_NAME
pipeline = cellprofiler.pipeline.Pipeline()
def callback(caller, event):
assert not isinstance(event, cellprofiler.pipeline.RunExceptionEvent)
pipeline.add_listener(callback)
pipeline.add_module(module)
image_set_list = cellprofiler.image.ImageSetList()
for i, image in enumerate(images):
image_set = image_set_list.get_image_set(i)
image_set.add(INPUT_IMAGE_NAME, cellprofiler.image.Image(image))
workspace = cellprofiler.workspace.Workspace(
pipeline,
module,
image_set_list.get_image_set(0),
cellprofiler.object.ObjectSet(),
cellprofiler.measurement.Measurements(),
image_set_list,
)
return workspace, module
def check(self, module, url, dd, keys=None, xml=None):
"""Check that running the metadata module on a url generates the expected dictionary"""
pipeline = cellprofiler.pipeline.Pipeline()
imgs = cellprofiler.modules.images.Images()
imgs.filter_choice.value = cellprofiler.modules.images.FILTER_CHOICE_NONE
imgs.module_num = 1
pipeline.add_module(imgs)
module.module_num = 2
pipeline.add_module(module)
pipeline.add_urls([url])
m = cellprofiler.measurement.Measurements()
workspace = cellprofiler.workspace.Workspace(pipeline, module, None,
None, m, None)
file_list = workspace.file_list
file_list.add_files_to_filelist([url])
if xml is not None:
file_list.add_metadata(url, xml)
ipds = pipeline.get_image_plane_details(workspace)
self.assertEqual(len(ipds), len(dd))
for d, ipd in zip(dd, ipds):
self.assertDictContainsSubset(d, ipd.metadata)
all_keys = list(pipeline.get_available_metadata_keys().keys())
if keys is not None:
for key in keys:
self.assertIn(key, all_keys)
def test_04_01_load_file(self):
csv_text = '''"Image_FileName_DNA","Image_PathName_DNA"
"%s","%s"
''' % (self.test_filename, self.test_path)
pipeline, module, filename = self.make_pipeline(csv_text)
c0_ran = [False]
def callback(workspace):
imgset = workspace.image_set
image = imgset.get_image("DNA")
pixels = image.pixel_data
self.assertEqual(pixels.shape[0], self.test_shape[0])
c0_ran[0] = True
c0 = C0()
c0.callback = callback
c0.module_num = 2
pipeline.add_module(c0)
try:
m = pipeline.run()
self.assertTrue(isinstance(m, cellprofiler.measurement.Measurements))
self.assertTrue(c0_ran[0])
hexdigest = m.get_current_image_measurement('MD5Digest_DNA')
self.assertEqual(hexdigest, self.test_md5)
self.assertTrue('PathName_DNA' in m.get_feature_names(cellprofiler.measurement.IMAGE))
self.assertEqual(m.get_current_image_measurement('PathName_DNA'),
self.test_path)
self.assertTrue('FileName_DNA' in m.get_feature_names(cellprofiler.measurement.IMAGE))
self.assertEqual(m.get_current_image_measurement('FileName_DNA'),
self.test_filename)
finally:
os.remove(filename)
def test_non_contiguous():
"""make sure MeasureObjectAreaShape doesn't crash if fed non-contiguous objects"""
module = cellprofiler.modules.measureobjectsizeshape.MeasureObjectAreaShape()
module.object_groups[0].name.value = "SomeObjects"
module.calculate_zernikes.value = True
object_set = cellprofiler.object.ObjectSet()
labels = numpy.zeros((10, 20), int)
labels[1:9, 1:5] = 1
labels[4:6, 6:19] = 1
objects = cellprofiler.object.Objects()
objects.segmented = labels
object_set.add_objects(objects, "SomeObjects")
module.set_module_num(1)
image_set_list = cellprofiler.image.ImageSetList()
measurements = cellprofiler.measurement.Measurements()
pipeline = cellprofiler.pipeline.Pipeline()
pipeline.add_module(module)
def callback(caller, event):
assert not isinstance(event, cellprofiler.pipeline.RunExceptionEvent)
pipeline.add_listener(callback)
workspace = cellprofiler.workspace.Workspace(
pipeline,
module,
image_set_list.get_image_set(0),
object_set,
measurements,
image_set_list,
)
module.run(workspace)
values = measurements.get_current_measurement("SomeObjects", "AreaShape_Perimeter")
assert len(values) == 1
assert values[0] == 54
def test_03_01_non_contiguous(self):
'''make sure MeasureObjectAreaShape doesn't crash if fed non-contiguous objects'''
module = cellprofiler.modules.measureobjectsizeshape.MeasureObjectAreaShape()
module.object_groups[0].name.value = "SomeObjects"
module.calculate_zernikes.value = True
object_set = cellprofiler.object.ObjectSet()
labels = numpy.zeros((10, 20), int)
labels[1:9, 1:5] = 1
labels[4:6, 6:19] = 1
objects = cellprofiler.object.Objects()
objects.segmented = labels
object_set.add_objects(objects, "SomeObjects")
module.module_num = 1
image_set_list = cellprofiler.image.ImageSetList()
measurements = cellprofiler.measurement.Measurements()
pipeline = cellprofiler.pipeline.Pipeline()
pipeline.add_module(module)
def callback(caller, event):
self.assertFalse(isinstance(event, cellprofiler.pipeline.RunExceptionEvent))
pipeline.add_listener(callback)
workspace = cellprofiler.workspace.Workspace(pipeline, module,
image_set_list.get_image_set(0),
object_set, measurements, image_set_list)
module.run(workspace)
values = measurements.get_current_measurement("SomeObjects",
"AreaShape_Perimeter")
self.assertEqual(len(values), 1)
self.assertEqual(values[0], 54)
def make_workspace(labels, mode, distance=0, neighbors_labels=None):
"""Make a workspace for testing MeasureObjectNeighbors"""
module = cellprofiler.modules.measureobjectneighbors.MeasureObjectNeighbors(
)
module.set_module_num(1)
module.object_name.value = OBJECTS_NAME
module.distance_method.value = mode
module.distance.value = distance
pipeline = cellprofiler.pipeline.Pipeline()
pipeline.add_module(module)
object_set = cellprofiler.object.ObjectSet()
image_set_list = cellprofiler.image.ImageSetList()
image_set = image_set_list.get_image_set(0)
measurements = cellprofiler.measurement.Measurements()
measurements.group_index = 1
measurements.group_number = 1
workspace = cellprofiler.workspace.Workspace(pipeline, module, image_set,
object_set, measurements,
image_set_list)
objects = cellprofiler.object.Objects()
objects.segmented = labels
object_set.add_objects(objects, OBJECTS_NAME)
if neighbors_labels is None:
module.neighbors_name.value = OBJECTS_NAME
else:
module.neighbors_name.value = NEIGHBORS_NAME
objects = cellprofiler.object.Objects()
objects.segmented = neighbors_labels
object_set.add_objects(objects, NEIGHBORS_NAME)
return workspace, module
def test_04_01_extent(self):
module = cellprofiler.modules.measureobjectsizeshape.MeasureObjectAreaShape(
)
module.object_groups[0].name.value = "SomeObjects"
module.calculate_zernikes.value = True
object_set = cellprofiler.object.ObjectSet()
labels = numpy.zeros((10, 20), int)
# 3/4 of a square is covered
labels[5:7, 5:10] = 1
labels[7:9, 5:15] = 1
objects = cellprofiler.object.Objects()
objects.segmented = labels
object_set.add_objects(objects, "SomeObjects")
module.module_num = 1
image_set_list = cellprofiler.image.ImageSetList()
measurements = cellprofiler.measurement.Measurements()
pipeline = cellprofiler.pipeline.Pipeline()
pipeline.add_module(module)
def callback(caller, event):
self.assertFalse(
isinstance(event, cellprofiler.pipeline.RunExceptionEvent))
pipeline.add_listener(callback)
workspace = cellprofiler.workspace.Workspace(
pipeline, module, image_set_list.get_image_set(0), object_set,
measurements, image_set_list)
module.run(workspace)
values = measurements.get_current_measurement(
"SomeObjects", "_".join(
(cellprofiler.modules.measureobjectsizeshape.AREA_SHAPE,
cellprofiler.modules.measureobjectsizeshape.F_EXTENT)))
self.assertEqual(len(values), 1)
self.assertAlmostEqual(values[0], .75)
def make_workspace(self,
pixel_data,
mask=None,
objects=None,
dimensions=2):
image_set_list = cellprofiler.image.ImageSetList()
image_set = image_set_list.get_image_set(0)
object_set = cellprofiler.object.ObjectSet()
image = cellprofiler.image.Image(pixel_data, dimensions=dimensions)
if not mask is None:
image.mask = mask
image_set.add(IMAGES_NAME, image)
if not objects is None:
o = cellprofiler.object.Objects()
o.segmented = objects
object_set.add_objects(o, OBJECTS_NAME)
module = M.MeasureImageQuality()
module.images_choice.value = M.O_SELECT
module.image_groups[0].include_image_scalings.value = False
module.image_groups[0].image_names.value = IMAGES_NAME
module.image_groups[0].use_all_threshold_methods.value = False
module.module_num = 1
pipeline = cellprofiler.pipeline.Pipeline()
pipeline.add_module(module)
workspace = cellprofiler.workspace.Workspace(
pipeline, module, image_set, object_set,
cellprofiler.measurement.Measurements(), image_set_list)
return workspace
def make_workspace(self,
labels,
operation,
iterations=1,
wants_outlines=False,
wants_fill_holes=False):
object_set = cellprofiler.object.ObjectSet()
objects = cellprofiler.object.Objects()
objects.segmented = labels
object_set.add_objects(objects, INPUT_NAME)
module = cellprofiler.modules.expandorshrinkobjects.ExpandOrShrink()
module.object_name.value = INPUT_NAME
module.output_object_name.value = OUTPUT_NAME
module.outlines_name.value = OUTLINES_NAME
module.operation.value = operation
module.iterations.value = iterations
module.wants_outlines.value = wants_outlines
module.wants_fill_holes.value = wants_fill_holes
module.module_num = 1
pipeline = cellprofiler.pipeline.Pipeline()
pipeline.add_module(module)
image_set_list = cellprofiler.image.ImageSetList()
workspace = cellprofiler.workspace.Workspace(
pipeline, module, image_set_list.get_image_set(0), object_set,
cellprofiler.measurement.Measurements(), image_set_list)
return workspace, module
def test_split_all():
img = get_my_image()
inj = cellprofiler.modules.injectimage.InjectImage("my_image", img)
inj.set_module_num(1)
ctg = cellprofiler.modules.colortogray.ColorToGray()
ctg.set_module_num(2)
ctg.image_name.value = "my_image"
ctg.combine_or_split.value = cellprofiler.modules.colortogray.SPLIT
ctg.use_red.value = True
ctg.use_blue.value = True
ctg.use_green.value = True
ctg.red_name.value = "my_red"
ctg.green_name.value = "my_green"
ctg.blue_name.value = "my_blue"
pipeline = cellprofiler.pipeline.Pipeline()
pipeline.add_module(inj)
pipeline.add_module(ctg)
pipeline.test_valid()
measurements = cellprofiler.measurement.Measurements()
object_set = cellprofiler.object.ObjectSet()
image_set_list = cellprofiler.image.ImageSetList()
workspace = cellprofiler.workspace.Workspace(
pipeline, inj, None, None, measurements, image_set_list, None
)
inj.prepare_run(workspace)
inj.prepare_group(workspace, {}, [1])
image_set = image_set_list.get_image_set(0)
inj.run(
cellprofiler.workspace.Workspace(
pipeline, inj, image_set, object_set, measurements, None
)
)
ctg.run(
cellprofiler.workspace.Workspace(
pipeline, ctg, image_set, object_set, measurements, None
)
)
red = image_set.get_image("my_red")
assert red
img = red.image
numpy.testing.assert_almost_equal(img[0, 0], 1)
numpy.testing.assert_almost_equal(img[0, 25], 0)
numpy.testing.assert_almost_equal(img[25, 0], 0)
numpy.testing.assert_almost_equal(img[25, 25], 0)
green = image_set.get_image("my_green")
assert green
img = green.image
numpy.testing.assert_almost_equal(img[0, 0], 0)
numpy.testing.assert_almost_equal(img[0, 25], 1)
numpy.testing.assert_almost_equal(img[25, 0], 0)
numpy.testing.assert_almost_equal(img[25, 25], 0)
blue = image_set.get_image("my_blue")
assert blue
img = blue.image
numpy.testing.assert_almost_equal(img[0, 0], 0)
numpy.testing.assert_almost_equal(img[0, 25], 0)
numpy.testing.assert_almost_equal(img[25, 0], 1)
numpy.testing.assert_almost_equal(img[25, 25], 0)
def test_05_01_overlapping(self):
'''Test object measurement with two overlapping objects in ijv format'''
i, j = numpy.mgrid[0:10, 0:20]
m = (i > 1) & (i < 9) & (j > 1) & (j < 19)
m1 = m & (i < j)
m2 = m & (i < 9 - j)
mlist = []
olist = []
for m in (m1, m2):
objects = cellprofiler.object.Objects()
objects.segmented = m.astype(int)
olist.append(objects)
ijv = numpy.column_stack((
numpy.hstack([numpy.argwhere(m)[:, 0] for m in (m1, m2)]),
numpy.hstack([numpy.argwhere(m)[:, 1] for m in (m1, m2)]),
numpy.array([1] * numpy.sum(m1) + [2] * numpy.sum(m2))))
objects = cellprofiler.object.Objects()
objects.ijv = ijv
olist.append(objects)
for objects in olist:
module = cellprofiler.modules.measureobjectsizeshape.MeasureObjectAreaShape()
module.object_groups[0].name.value = "SomeObjects"
module.calculate_zernikes.value = True
object_set = cellprofiler.object.ObjectSet()
object_set.add_objects(objects, "SomeObjects")
module.module_num = 1
image_set_list = cellprofiler.image.ImageSetList()
measurements = cellprofiler.measurement.Measurements()
mlist.append(measurements)
pipeline = cellprofiler.pipeline.Pipeline()
pipeline.add_module(module)
def callback(caller, event):
self.assertFalse(isinstance(event, cellprofiler.pipeline.RunExceptionEvent))
pipeline.add_listener(callback)
workspace = cellprofiler.workspace.Workspace(pipeline, module,
image_set_list.get_image_set(0),
object_set, measurements, image_set_list)
module.run(workspace)
pipeline = cellprofiler.pipeline.Pipeline()
for c in module.get_measurement_columns(pipeline):
oname, feature = c[:2]
if oname != "SomeObjects":
continue
measurements = mlist[0]
self.assertTrue(isinstance(measurements, cellprofiler.measurement.Measurements))
v1 = measurements.get_current_measurement(oname, feature)
self.assertEqual(len(v1), 1)
v1 = v1[0]
measurements = mlist[1]
v2 = measurements.get_current_measurement(oname, feature)
self.assertEqual(len(v2), 1)
v2 = v2[0]
expected = (v1, v2)
v = mlist[2].get_current_measurement(oname, feature)
self.assertEqual(tuple(v), expected)
def run_image_set(projection_type, images_and_masks, frequency=9, run_last=True):
image_set_list = cellprofiler.image.ImageSetList()
image_count = len(images_and_masks)
for i in range(image_count):
pixel_data, mask = images_and_masks[i]
if mask is None:
image = cellprofiler.image.Image(pixel_data)
else:
image = cellprofiler.image.Image(pixel_data, mask)
image_set_list.get_image_set(i).add(IMAGE_NAME, image)
#
# Add bogus image at end for 2nd group
#
bogus_image = cellprofiler.image.Image(numpy.zeros((10, 20)))
image_set_list.get_image_set(image_count).add(IMAGE_NAME, bogus_image)
pipeline = cellprofiler.pipeline.Pipeline()
module = cellprofiler.modules.makeprojection.MakeProjection()
module.set_module_num(1)
module.image_name.value = IMAGE_NAME
module.projection_image_name.value = PROJECTED_IMAGE_NAME
module.projection_type.value = projection_type
module.frequency.value = frequency
pipeline.add_module(module)
m = cellprofiler.measurement.Measurements()
workspace = cellprofiler.workspace.Workspace(
pipeline, module, None, None, m, image_set_list
)
module.prepare_run(workspace)
module.prepare_group(workspace, {}, list(range(1, len(images_and_masks) + 1)))
for i in range(image_count):
if i > 0:
image_set_list.purge_image_set(i - 1)
w = cellprofiler.workspace.Workspace(
pipeline,
module,
image_set_list.get_image_set(i),
cellprofiler.object.ObjectSet(),
m,
image_set_list,
)
if i < image_count - 1 or run_last:
module.run(w)
module.post_group(w, {})
image = w.image_set.get_image(PROJECTED_IMAGE_NAME)
#
# Make sure that the image provider is reset after prepare_group
#
module.prepare_group(workspace, {}, [image_count + 1])
image_set = image_set_list.get_image_set(image_count)
w = cellprofiler.workspace.Workspace(
pipeline, module, image_set, cellprofiler.object.ObjectSet(), m, image_set_list
)
module.run(w)
image_provider = image_set.get_image_provider(PROJECTED_IMAGE_NAME)
assert numpy.max(image_provider.count) == 1
return image
def make_workspace(self, parents, children, fake_measurement=False):
"""Make a workspace for testing Relate"""
pipeline = cellprofiler.pipeline.Pipeline()
if fake_measurement:
class FakeModule(cellprofiler.module.Module):
def get_measurement_columns(self, pipeline):
return [
(
CHILD_OBJECTS,
MEASUREMENT,
cellprofiler.measurement.COLTYPE_FLOAT,
),
(
CHILD_OBJECTS,
IGNORED_MEASUREMENT,
cellprofiler.measurement.COLTYPE_INTEGER,
),
]
module = FakeModule()
module.set_module_num(1)
pipeline.add_module(module)
module = cellprofiler.modules.relateobjects.Relate()
module.x_name.value = PARENT_OBJECTS
module.y_name.value = CHILD_OBJECTS
module.find_parent_child_distances.value = (
cellprofiler.modules.relateobjects.D_NONE)
module.wants_child_objects_saved.value = False
new_module_num = 2 if fake_measurement else 1
module.set_module_num(new_module_num)
pipeline.add_module(module)
object_set = cellprofiler.object.ObjectSet()
image_set_list = cellprofiler.image.ImageSetList()
image_set = image_set_list.get_image_set(0)
m = cellprofiler.measurement.Measurements()
m.add_image_measurement(cellprofiler.measurement.GROUP_NUMBER, 1)
m.add_image_measurement(cellprofiler.measurement.GROUP_INDEX, 1)
workspace = cellprofiler.workspace.Workspace(pipeline, module,
image_set, object_set, m,
image_set_list)
o = cellprofiler.object.Objects()
if parents.shape[1] == 3:
# IJV format
o.ijv = parents
else:
o.segmented = parents
object_set.add_objects(o, PARENT_OBJECTS)
o = cellprofiler.object.Objects()
if children.shape[1] == 3:
o.ijv = children
else:
o.segmented = children
object_set.add_objects(o, CHILD_OBJECTS)
return workspace, module
def test_prepare_run():
# regression test for issue #673 and #1161
#
# If LoadSingleImage appears first, pathname data does not show
# up in the measurements.
#
tests.modules.maybe_download_sbs()
folder = "ExampleSBSImages"
path = os.path.join(tests.modules.example_images_directory(), folder)
filename = "Channel1-01-A-01.tif"
pipeline = cellprofiler.pipeline.Pipeline()
lsi = cellprofiler.modules.loadsingleimage.LoadSingleImage()
lsi.set_module_num(1)
lsi.directory.dir_choice = cellprofiler.setting.ABSOLUTE_FOLDER_NAME
lsi.directory.custom_path = path
lsi.file_settings[0].image_name.value = get_image_name(0)
lsi.file_settings[0].file_name.value = filename
pipeline.add_module(lsi)
li = cellprofiler.modules.loadimages.LoadImages()
li.set_module_num(2)
pipeline.add_module(li)
li.match_method.value = cellprofiler.modules.loadimages.MS_EXACT_MATCH
li.location.dir_choice = cellprofiler.setting.ABSOLUTE_FOLDER_NAME
li.location.custom_path = path
li.images[0].common_text.value = "Channel2-"
m = cellprofiler.measurement.Measurements()
workspace = cellprofiler.workspace.Workspace(
pipeline,
lsi,
m,
cellprofiler.object.ObjectSet(),
m,
cellprofiler.image.ImageSetList(),
)
assert pipeline.prepare_run(workspace)
assert m.image_set_count > 1
pipeline.prepare_group(workspace, {}, m.get_image_numbers())
#
# Skip to the second image set
#
m.next_image_set(2)
lsi.run(workspace)
#
# Are the measurements populated?
#
m_file = "_".join(
(cellprofiler.measurement.C_FILE_NAME, get_image_name(0)))
assert m[cellprofiler.measurement.IMAGE, m_file, 2] == filename
#
# Can we retrieve the image?
#
pixel_data = m.get_image(get_image_name(0)).pixel_data
assert not numpy.isscalar(pixel_data)
def make_pipeline(
image,
mask,
subsample_size,
image_sample_size,
element_size,
granular_spectrum_length,
labels=None,
):
"""Make a pipeline with a MeasureGranularity module
image - measure granularity on this image
mask - exclude / include pixels from measurement. None = no mask
subsample_size, etc. - values for corresponding settings in the module
returns tuple of module & workspace
"""
module = cellprofiler.modules.measuregranularity.MeasureGranularity()
module.set_module_num(1)
image_setting = module.images[0]
# assert isinstance(image_setting, M.MeasureGranularity)
image_setting.image_name.value = IMAGE_NAME
image_setting.subsample_size.value = subsample_size
image_setting.image_sample_size.value = image_sample_size
image_setting.element_size.value = element_size
image_setting.granular_spectrum_length.value = granular_spectrum_length
image_set_list = cellprofiler.image.ImageSetList()
image_set = image_set_list.get_image_set(0)
img = cellprofiler.image.Image(image, mask)
image_set.add(IMAGE_NAME, img)
pipeline = cellprofiler.pipeline.Pipeline()
def error_callback(event, caller):
assert not isinstance(event, cellprofiler.pipeline.RunExceptionEvent)
pipeline.add_listener(error_callback)
pipeline.add_module(module)
object_set = cellprofiler.object.ObjectSet()
if labels is not None:
objects = cellprofiler.object.Objects()
objects.segmented = labels
object_set.add_objects(objects, OBJECTS_NAME)
image_setting.add_objects()
image_setting.objects[0].objects_name.value = OBJECTS_NAME
workspace = cellprofiler.workspace.Workspace(
pipeline,
module,
image_set,
object_set,
cellprofiler.measurement.Measurements(),
image_set_list,
)
return module, workspace
def make_workspace(labels,
overlap_choice,
masking_objects=None,
masking_image=None,
renumber=True):
module = cellprofiler.modules.maskobjects.MaskObjects()
module.set_module_num(1)
module.object_name.value = INPUT_OBJECTS
module.remaining_objects.value = OUTPUT_OBJECTS
module.mask_choice.value = (cellprofiler.modules.maskobjects.MC_OBJECTS
if masking_objects is not None else
cellprofiler.modules.maskobjects.MC_IMAGE)
module.masking_image.value = MASKING_IMAGE
module.masking_objects.value = MASKING_OBJECTS
module.retain_or_renumber.value = (
cellprofiler.modules.maskobjects.R_RENUMBER
if renumber else cellprofiler.modules.maskobjects.R_RETAIN)
module.overlap_choice.value = overlap_choice
pipeline = cellprofiler.pipeline.Pipeline()
def callback(caller, event):
assert not isinstance(event, cellprofiler.pipeline.RunExceptionEvent)
pipeline.add_listener(callback)
pipeline.add_module(module)
object_set = cellprofiler.object.ObjectSet()
io = cellprofiler.object.Objects()
io.segmented = labels
object_set.add_objects(io, INPUT_OBJECTS)
if masking_objects is not None:
oo = cellprofiler.object.Objects()
oo.segmented = masking_objects
object_set.add_objects(oo, MASKING_OBJECTS)
image_set_list = cellprofiler.image.ImageSetList()
image_set = image_set_list.get_image_set(0)
if masking_image is not None:
mi = cellprofiler.image.Image(masking_image)
image_set.add(MASKING_IMAGE, mi)
workspace = cellprofiler.workspace.Workspace(
pipeline,
module,
image_set,
object_set,
cellprofiler.measurement.Measurements(),
image_set_list,
)
return workspace, module
def test_04_01_load_objects(self):
r = numpy.random.RandomState()
r.seed(41)
labels = numpy.random.randint(0, 10, size=(30, 40))
filename = "myobjects.png"
directory = tempfile.mkdtemp()
cellprofiler.preferences.set_default_image_directory(directory)
pilimage = PIL.Image.fromarray(labels.astype(numpy.uint8), "L")
pilimage.save(os.path.join(directory, filename))
del pilimage
try:
module = cellprofiler.modules.loadsingleimage.LoadSingleImage()
module.module_num = 1
module.directory.set_dir_choice(cellprofiler.modules.loadsingleimage.DEFAULT_INPUT_FOLDER_NAME)
fs = module.file_settings[0]
fs.file_name.value = filename
fs.image_objects_choice.value = cellprofiler.modules.loadsingleimage.IO_OBJECTS
fs.objects_name.value = OBJECTS_NAME
pipeline = cellprofiler.pipeline.Pipeline()
def callback(caller, event):
self.assertFalse(isinstance(event, cellprofiler.pipeline.RunExceptionEvent))
pipeline.add_listener(callback)
pipeline.add_module(module)
m = cellprofiler.measurement.Measurements()
object_set = cellprofiler.object.ObjectSet()
image_set_list = cellprofiler.image.ImageSetList()
image_set = image_set_list.get_image_set(0)
workspace = cellprofiler.workspace.Workspace(
pipeline, module, image_set, object_set, m, image_set_list)
module.prepare_run(workspace)
module.run(workspace)
o = object_set.get_objects(OBJECTS_NAME)
numpy.testing.assert_equal(labels, o.segmented)
self.assertEqual(m.get_current_image_measurement(
"_".join((cellprofiler.measurement.C_COUNT, OBJECTS_NAME))), 9)
self.assertEqual(m.get_current_image_measurement(
"_".join((cellprofiler.modules.loadsingleimage.C_OBJECTS_FILE_NAME, OBJECTS_NAME))), filename)
self.assertEqual(m.get_current_image_measurement(
"_".join((cellprofiler.modules.loadsingleimage.C_OBJECTS_PATH_NAME, OBJECTS_NAME))), directory)
for feature in (cellprofiler.measurement.M_LOCATION_CENTER_X, cellprofiler.measurement.M_LOCATION_CENTER_Y,
cellprofiler.measurement.M_NUMBER_OBJECT_NUMBER):
values = m.get_current_measurement(OBJECTS_NAME, feature)
self.assertEqual(len(values), 9)
finally:
try:
os.remove(os.path.join(directory, filename))
os.rmdir(directory)
except:
print("Failed to delete directory " + directory)
def test_09_01_load_bcb_file(self):
csv_text = """ELN_RUN_ID,CBIP_RUN_ID,ASSAY_PLATE_BARCODE,\
MX_PLATE_ID,ASSAY_WELL_POSITION,ASSAY_WELL_ROLE,SITE_X,SITE_Y,\
MICROSCOPE,SOURCE_DESCRIPTION,DATE_CREATED,FILE_PATH,FILE_NAME,\
CPD_PLATE_MAP_NAME,CPD_WELL_POSITION,BROAD_ID,\
CPD_MMOL_CONC,SOURCE_NAME,SOURCE_COMPOUND_NAME,CPD_SMILES
"4012-10-W01-01-02","4254","BR00021547","20777","N01","COMPOUND",\
"2","2","GS IX Micro","DAPI","2010/03/19 06:01:12","%s",\
"%s","C-4012-00-D80-001_Rev3","N01",\
"BRD-K71194192-001-01-6","2.132352941","ChemBridge","",\
"Oc1ccnc(SCC(=O)Nc2ccc(Oc3ccccc3)cc2)n1"
""" % (
self.test_path,
self.test_filename,
)
pipeline, module, filename = self.make_pipeline(csv_text)
c0_ran = [False]
def callback(workspace):
imgset = workspace.image_set
image = imgset.get_image("DAPI")
pixels = image.pixel_data
self.assertEqual(pixels.shape[0], self.test_shape[0])
c0_ran[0] = True
c0 = C0()
c0.callback = callback
c0.module_num = 2
pipeline.add_module(c0)
try:
m = pipeline.run()
self.assertTrue(isinstance(m, cellprofiler.measurement.Measurements))
self.assertTrue(c0_ran[0])
hexdigest = m.get_current_image_measurement("MD5Digest_DAPI")
self.assertEqual(hexdigest, self.test_md5)
self.assertTrue(
"PathName_DAPI" in m.get_feature_names(cellprofiler.measurement.IMAGE)
)
self.assertEqual(
m.get_current_image_measurement("PathName_DAPI"), self.test_path
)
self.assertTrue(
"FileName_DAPI" in m.get_feature_names(cellprofiler.measurement.IMAGE)
)
self.assertEqual(
m.get_current_image_measurement("FileName_DAPI"), self.test_filename
)
finally:
os.remove(filename)
def test_06_get_settings_list(self):
module1 = instantiate_module("IdentifyPrimaryObjects")
module1.module_num = 1
module2 = instantiate_module("Smooth")
module2.module_num = 2
module3 = instantiate_module("Resize")
module3.module_num = 3
module4 = self.make_instance()
module4.module_num = 4
module4.parameters[0].module_names.value = "IdentifyPrimaryObjects #1"
module4.add_parameter()
module4.parameters[1].module_names.value = "Smooth #2"
module4.add_parameter()
module4.parameters[2].module_names.value = "Resize #3"
module4.add_parameter()
module4.parameters[3].module_names.value = "BayesianOptimisation #4"
pipeline = cellprofiler.pipeline.Pipeline()
pipeline.add_module(module1)
pipeline.add_module(module2)
pipeline.add_module(module3)
pipeline.add_module(module4)
settings_list = module4.get_settings_from_modules(pipeline)
self.assertTrue("Use advanced settings?" in settings_list)
self.assertTrue("Select smoothing method" in settings_list)
self.assertTrue("Resizing factor" in settings_list)
self.assertTrue("No. of settings to be adjusted" in settings_list)
def test_01_02_split_all():
img = get_my_image()
inj = cellprofiler.modules.injectimage.InjectImage("my_image", img)
inj.module_num = 1
ctg = cellprofiler.modules.colortogray.ColorToGray()
ctg.module_num = 2
ctg.image_name.value = "my_image"
ctg.combine_or_split.value = cellprofiler.modules.colortogray.SPLIT
ctg.use_red.value = True
ctg.use_blue.value = True
ctg.use_green.value = True
ctg.red_name.value = "my_red"
ctg.green_name.value = "my_green"
ctg.blue_name.value = "my_blue"
pipeline = cellprofiler.pipeline.Pipeline()
pipeline.add_module(inj)
pipeline.add_module(ctg)
pipeline.test_valid()
measurements = cellprofiler.measurement.Measurements()
object_set = cellprofiler.object.ObjectSet()
image_set_list = cellprofiler.image.ImageSetList()
workspace = Workspace(pipeline, inj, None, None, measurements,
image_set_list, None)
inj.prepare_run(workspace)
inj.prepare_group(workspace, {}, [1])
image_set = image_set_list.get_image_set(0)
inj.run(Workspace(pipeline, inj, image_set, object_set, measurements, None))
ctg.run(Workspace(pipeline, ctg, image_set, object_set, measurements, None))
red = image_set.get_image("my_red")
assert red
img = red.image
numpy.testing.assert_almost_equal(img[0, 0], 1)
numpy.testing.assert_almost_equal(img[0, 25], 0)
numpy.testing.assert_almost_equal(img[25, 0], 0)
numpy.testing.assert_almost_equal(img[25, 25], 0)
green = image_set.get_image("my_green")
assert green
img = green.image
numpy.testing.assert_almost_equal(img[0, 0], 0)
numpy.testing.assert_almost_equal(img[0, 25], 1)
numpy.testing.assert_almost_equal(img[25, 0], 0)
numpy.testing.assert_almost_equal(img[25, 25], 0)
blue = image_set.get_image("my_blue")
assert blue
img = blue.image
numpy.testing.assert_almost_equal(img[0, 0], 0)
numpy.testing.assert_almost_equal(img[0, 25], 0)
numpy.testing.assert_almost_equal(img[25, 0], 1)
numpy.testing.assert_almost_equal(img[25, 25], 0)
def check(self, module, url, expected):
'''Check filtering of one URL using the module as configured'''
pipeline = cellprofiler.pipeline.Pipeline()
pipeline.add_urls([url])
module.module_num = 1
pipeline.add_module(module)
m = cellprofiler.measurement.Measurements()
workspace = cellprofiler.workspace.Workspace(pipeline, module, None, None, m, None)
file_list = pipeline.get_filtered_file_list(workspace)
if expected:
self.assertEqual(len(file_list), 1)
self.assertEqual(file_list[0], url)
else:
self.assertEqual(len(file_list), 0)
def make_obj_workspace(self, ground_truth_obj, id_obj, ground_truth, id):
"""make a workspace to test comparing objects"""
""" ground truth object and ID object are dictionaires w/ the following keys"""
"""i - i component of pixel coordinates
j - j component of pixel coordinates
l - label """
module = cellprofiler.modules.measureobjectoverlap.MeasureObjectOverlap(
)
module.module_num = 1
module.object_name_GT.value = GROUND_TRUTH_OBJ
module.object_name_ID.value = ID_OBJ
module.wants_emd.value = True
pipeline = cellprofiler.pipeline.Pipeline()
def callback(caller, event):
self.assertFalse(
isinstance(event, cellprofiler.pipeline.RunExceptionEvent))
pipeline.add_listener(callback)
pipeline.add_module(module)
image_set_list = cellprofiler.image.ImageSetList()
image_set = image_set_list.get_image_set(0)
for name, d in (
(GROUND_TRUTH_OBJ_IMAGE_NAME, ground_truth),
(ID_OBJ_IMAGE_NAME, id),
):
image = cellprofiler.image.Image(d["image"],
mask=d.get("mask"),
crop_mask=d.get("crop_mask"))
image_set.add(name, image)
object_set = cellprofiler.object.ObjectSet()
for name, d in ((GROUND_TRUTH_OBJ, ground_truth_obj), (ID_OBJ,
id_obj)):
object = cellprofiler.object.Objects()
if d.shape[1] == 3:
object.ijv = d
else:
object.segmented = d
object_set.add_objects(object, name)
workspace = cellprofiler.workspace.Workspace(
pipeline,
module,
image_set,
object_set,
cellprofiler.measurement.Measurements(),
image_set_list,
)
return workspace, module
def test_02_03_prepare_run(self):
# regression test for issue #673 and #1161
#
# If LoadSingleImage appears first, pathname data does not show
# up in the measurements.
#
tests.modules.maybe_download_sbs()
folder = "ExampleSBSImages"
path = os.path.join(tests.modules.example_images_directory(), folder)
filename = "Channel1-01-A-01.tif"
pipeline = cellprofiler.pipeline.Pipeline()
lsi = cellprofiler.modules.loadsingleimage.LoadSingleImage()
lsi.module_num = 1
lsi.directory.dir_choice = cellprofiler.setting.ABSOLUTE_FOLDER_NAME
lsi.directory.custom_path = path
lsi.file_settings[0].image_name.value = self.get_image_name(0)
lsi.file_settings[0].file_name.value = filename
pipeline.add_module(lsi)
li = cellprofiler.modules.loadimages.LoadImages()
li.module_num = 2
pipeline.add_module(li)
li.match_method.value = cellprofiler.modules.loadimages.MS_EXACT_MATCH
li.location.dir_choice = cellprofiler.setting.ABSOLUTE_FOLDER_NAME
li.location.custom_path = path
li.images[0].common_text.value = "Channel2-"
m = cellprofiler.measurement.Measurements()
workspace = cellprofiler.workspace.Workspace(pipeline, lsi, m, cellprofiler.object.ObjectSet(), m,
cellprofiler.image.ImageSetList())
self.assertTrue(pipeline.prepare_run(workspace))
self.assertGreater(m.image_set_count, 1)
pipeline.prepare_group(workspace, {}, m.get_image_numbers())
#
# Skip to the second image set
#
m.next_image_set(2)
lsi.run(workspace)
#
# Are the measurements populated?
#
m_file = "_".join((cellprofiler.measurement.C_FILE_NAME, self.get_image_name(0)))
self.assertEqual(m[cellprofiler.measurement.IMAGE, m_file, 2], filename)
#
# Can we retrieve the image?
#
pixel_data = m.get_image(self.get_image_name(0)).pixel_data
self.assertFalse(numpy.isscalar(pixel_data))
def test_04_02_object_outlines(self):
labels = numpy.zeros((30, 40), int)
labels[10:15, 20:30] = 1
expected_outlines = labels != 0
expected_outlines[11:14, 21:29] = False
filename = "myobjects.png"
directory = tempfile.mkdtemp()
cellprofiler.preferences.set_default_image_directory(directory)
pilimage = PIL.Image.fromarray(labels.astype(numpy.uint8), "L")
pilimage.save(os.path.join(directory, filename))
del pilimage
try:
module = cellprofiler.modules.loadsingleimage.LoadSingleImage()
module.module_num = 1
module.directory.set_dir_choice(cellprofiler.modules.loadsingleimage.DEFAULT_INPUT_FOLDER_NAME)
fs = module.file_settings[0]
fs.file_name.value = filename
fs.image_objects_choice.value = cellprofiler.modules.loadsingleimage.IO_OBJECTS
fs.objects_name.value = OBJECTS_NAME
fs.wants_outlines.value = True
fs.outlines_name.value = OUTLINES_NAME
pipeline = cellprofiler.pipeline.Pipeline()
def callback(caller, event):
self.assertFalse(isinstance(event, cellprofiler.pipeline.RunExceptionEvent))
pipeline.add_listener(callback)
pipeline.add_module(module)
m = cellprofiler.measurement.Measurements()
object_set = cellprofiler.object.ObjectSet()
image_set_list = cellprofiler.image.ImageSetList()
image_set = image_set_list.get_image_set(0)
workspace = cellprofiler.workspace.Workspace(
pipeline, module, image_set, object_set, m, image_set_list)
module.prepare_run(workspace)
module.run(workspace)
outlines = image_set.get_image(OUTLINES_NAME)
numpy.testing.assert_equal(outlines.pixel_data, expected_outlines)
finally:
try:
os.remove(os.path.join(directory, filename))
os.rmdir(directory)
except:
print("Failed to delete directory " + directory)
def make_workspace(self, parents, children, fake_measurement=False):
'''Make a workspace for testing Relate'''
pipeline = cellprofiler.pipeline.Pipeline()
if fake_measurement:
class FakeModule(cellprofiler.module.Module):
def get_measurement_columns(self, pipeline):
return [(CHILD_OBJECTS, MEASUREMENT, cellprofiler.measurement.COLTYPE_FLOAT),
(CHILD_OBJECTS, IGNORED_MEASUREMENT, cellprofiler.measurement.COLTYPE_INTEGER)]
module = FakeModule()
module.module_num = 1
pipeline.add_module(module)
module = cellprofiler.modules.relateobjects.Relate()
module.x_name.value = PARENT_OBJECTS
module.x_child_name.value = CHILD_OBJECTS
module.find_parent_child_distances.value = cellprofiler.modules.relateobjects.D_NONE
module.module_num = 2 if fake_measurement else 1
pipeline.add_module(module)
object_set = cellprofiler.object.ObjectSet()
image_set_list = cellprofiler.image.ImageSetList()
image_set = image_set_list.get_image_set(0)
m = cellprofiler.measurement.Measurements()
m.add_image_measurement(cellprofiler.measurement.GROUP_NUMBER, 1)
m.add_image_measurement(cellprofiler.measurement.GROUP_INDEX, 1)
workspace = cellprofiler.workspace.Workspace(pipeline,
module,
image_set,
object_set,
m,
image_set_list)
o = cellprofiler.object.Objects()
if parents.shape[1] == 3:
# IJV format
o.ijv = parents
else:
o.segmented = parents
object_set.add_objects(o, PARENT_OBJECTS)
o = cellprofiler.object.Objects()
if children.shape[1] == 3:
o.ijv = children
else:
o.segmented = children
object_set.add_objects(o, CHILD_OBJECTS)
return workspace, module
def make_workspace(self, image, size_method, interpolation,
mask=None, cropping=None, dimensions=2):
module = cellprofiler.modules.resize.Resize()
module.x_name.value = INPUT_IMAGE_NAME
module.y_name.value = OUTPUT_IMAGE_NAME
module.size_method.value = size_method
module.interpolation.value = interpolation
module.module_num = 1
pipeline = cellprofiler.pipeline.Pipeline()
pipeline.add_module(module)
image_set_list = cellprofiler.image.ImageSetList()
image_set = image_set_list.get_image_set(0)
image = cellprofiler.image.Image(image, mask, cropping, dimensions=dimensions)
image_set.add(INPUT_IMAGE_NAME, image)
workspace = cellprofiler.workspace.Workspace(pipeline, module, image_set,
cellprofiler.object.ObjectSet(),
cellprofiler.measurement.Measurements(),
image_set_list)
return workspace, module
def make_workspace(self, labels, parent_image=None):
object_set = cellprofiler.object.ObjectSet()
objects = cellprofiler.object.Objects()
objects.segmented = labels
objects.parent_image = parent_image
object_set.add_objects(objects, OBJECTS_NAME)
pipeline = cellprofiler.pipeline.Pipeline()
module = cellprofiler.modules.measureimageareaoccupied.MeasureImageAreaOccupied()
module.module_num = 1
module.operands[0].operand_objects.value = OBJECTS_NAME
pipeline.add_module(module)
image_set_list = cellprofiler.image.ImageSetList()
workspace = cellprofiler.workspace.Workspace(pipeline, module,
image_set_list.get_image_set(0),
object_set,
cellprofiler.measurement.Measurements(),
image_set_list)
return workspace
def test_09_01_load_bcb_file(self):
csv_text = '''ELN_RUN_ID,CBIP_RUN_ID,ASSAY_PLATE_BARCODE,\
MX_PLATE_ID,ASSAY_WELL_POSITION,ASSAY_WELL_ROLE,SITE_X,SITE_Y,\
MICROSCOPE,SOURCE_DESCRIPTION,DATE_CREATED,FILE_PATH,FILE_NAME,\
CPD_PLATE_MAP_NAME,CPD_WELL_POSITION,BROAD_ID,\
CPD_MMOL_CONC,SOURCE_NAME,SOURCE_COMPOUND_NAME,CPD_SMILES
"4012-10-W01-01-02","4254","BR00021547","20777","N01","COMPOUND",\
"2","2","GS IX Micro","DAPI","2010/03/19 06:01:12","%s",\
"%s","C-4012-00-D80-001_Rev3","N01",\
"BRD-K71194192-001-01-6","2.132352941","ChemBridge","",\
"Oc1ccnc(SCC(=O)Nc2ccc(Oc3ccccc3)cc2)n1"
''' % (self.test_path, self.test_filename)
pipeline, module, filename = self.make_pipeline(csv_text)
c0_ran = [False]
def callback(workspace):
imgset = workspace.image_set
image = imgset.get_image("DAPI")
pixels = image.pixel_data
self.assertEqual(pixels.shape[0], self.test_shape[0])
c0_ran[0] = True
c0 = C0()
c0.callback = callback
c0.module_num = 2
pipeline.add_module(c0)
try:
m = pipeline.run()
self.assertTrue(isinstance(m, cellprofiler.measurement.Measurements))
self.assertTrue(c0_ran[0])
hexdigest = m.get_current_image_measurement('MD5Digest_DAPI')
self.assertEqual(hexdigest, self.test_md5)
self.assertTrue('PathName_DAPI' in m.get_feature_names(cellprofiler.measurement.IMAGE))
self.assertEqual(m.get_current_image_measurement('PathName_DAPI'),
self.test_path)
self.assertTrue('FileName_DAPI' in m.get_feature_names(cellprofiler.measurement.IMAGE))
self.assertEqual(m.get_current_image_measurement('FileName_DAPI'),
self.test_filename)
finally:
os.remove(filename)
def test_06_01_images_to_process(self):
#
# Test MeasureImageQuality.images_to_process on a pipeline with a
# variety of image providers.
#
expected_names = ["foo", "bar"]
pipeline = cellprofiler.pipeline.Pipeline()
module1 = cellprofiler.modules.namesandtypes.NamesAndTypes()
module1.module_num = 1
module1.assignment_method.value = \
cellprofiler.modules.namesandtypes.ASSIGN_RULES
module1.add_assignment()
module1.add_assignment()
module1.assignments[0].image_name.value = expected_names[0]
module1.assignments[0].load_as_choice.value = \
cellprofiler.modules.namesandtypes.LOAD_AS_GRAYSCALE_IMAGE
#
# TO_DO: issue #652
# This test should fail at some later date when we can detect
# that an illumination function should not be QA measured
#
module1.assignments[1].image_name.value = expected_names[1]
module1.assignments[1].load_as_choice.value = \
cellprofiler.modules.namesandtypes.LOAD_AS_ILLUMINATION_FUNCTION
module1.assignments[2].load_as_choice.value = \
cellprofiler.modules.namesandtypes.LOAD_AS_OBJECTS
pipeline.add_module(module1)
module2 = cellprofiler.modules.smooth.Smooth()
module2.module_num = 2
module2.image_name.value = expected_names[0]
module2.filtered_image_name.value = "henry"
pipeline.add_module(module2)
miq_module = M.MeasureImageQuality()
miq_module.module_num = 3
miq_module.images_choice.value = M.O_ALL_LOADED
image_names = miq_module.images_to_process(
miq_module.image_groups[0], None, pipeline)
self.assertEqual(len(image_names), len(expected_names))
for image_name in image_names:
self.assertTrue(image_name in expected_names)
def make_obj_workspace(self, ground_truth_obj, id_obj, ground_truth, id):
'''make a workspace to test comparing objects'''
''' ground truth object and ID object are dictionaires w/ the following keys'''
'''i - i component of pixel coordinates
j - j component of pixel coordinates
l - label '''
module = cellprofiler.modules.measureobjectoverlap.MeasureObjectOverlap()
module.module_num = 1
module.object_name_GT.value = GROUND_TRUTH_OBJ
module.object_name_ID.value = ID_OBJ
module.wants_emd.value = True
pipeline = cellprofiler.pipeline.Pipeline()
def callback(caller, event):
self.assertFalse(isinstance(event, cellprofiler.pipeline.RunExceptionEvent))
pipeline.add_listener(callback)
pipeline.add_module(module)
image_set_list = cellprofiler.image.ImageSetList()
image_set = image_set_list.get_image_set(0)
for name, d in ((GROUND_TRUTH_OBJ_IMAGE_NAME, ground_truth),
(ID_OBJ_IMAGE_NAME, id)):
image = cellprofiler.image.Image(d["image"],
mask=d.get("mask"),
crop_mask=d.get("crop_mask"))
image_set.add(name, image)
object_set = cellprofiler.object.ObjectSet()
for name, d in ((GROUND_TRUTH_OBJ, ground_truth_obj),
(ID_OBJ, id_obj)):
object = cellprofiler.object.Objects()
if d.shape[1] == 3:
object.ijv = d
else:
object.segmented = d
object_set.add_objects(object, name)
workspace = cellprofiler.workspace.Workspace(pipeline, module, image_set,
object_set, cellprofiler.measurement.Measurements(),
image_set_list)
return workspace, module
def make_workspace(self, ground_truth, test, dimensions=2):
'''Make a workspace with a ground-truth image and a test image
ground_truth and test are dictionaries with the following keys:
image - the pixel data
mask - (optional) the mask data
crop_mask - (optional) a cropping mask
returns a workspace and module
'''
module = cellprofiler.modules.measureimageoverlap.MeasureImageOverlap()
module.module_num = 1
module.ground_truth.value = GROUND_TRUTH_IMAGE_NAME
module.test_img.value = TEST_IMAGE_NAME
module.wants_emd.value = True
pipeline = cellprofiler.pipeline.Pipeline()
def callback(caller, event):
self.assertFalse(isinstance(event, cellprofiler.pipeline.RunExceptionEvent))
pipeline.add_listener(callback)
pipeline.add_module(module)
image_set_list = cellprofiler.image.ImageSetList()
image_set = image_set_list.get_image_set(0)
for name, d in ((GROUND_TRUTH_IMAGE_NAME, ground_truth),
(TEST_IMAGE_NAME, test)):
image = cellprofiler.image.Image(
d["image"],
mask=d.get("mask"),
crop_mask=d.get("crop_mask"),
dimensions=dimensions
)
image_set.add(name, image)
workspace = cellprofiler.workspace.Workspace(pipeline, module, image_set,
cellprofiler.object.ObjectSet(), cellprofiler.measurement.Measurements(),
image_set_list)
return workspace, module
def make_workspace(self, labels):
image_set_list = cellprofiler.image.ImageSetList()
image_set = image_set_list.get_image_set(0)
object_set = cellprofiler.object.ObjectSet()
objects = cellprofiler.object.Objects()
objects.segmented = labels
object_set.add_objects(objects, OBJECTS_NAME)
m = cellprofiler.measurement.Measurements()
module = cellprofiler.modules.measureobjectsizeshape.MeasureObjectAreaShape()
module.module_num = 1
module.object_groups[0].name.value = OBJECTS_NAME
pipeline = cellprofiler.pipeline.Pipeline()
def callback(caller, event):
self.assertFalse(isinstance(event, cellprofiler.pipeline.RunExceptionEvent))
pipeline.add_listener(callback)
pipeline.add_module(module)
workspace = cellprofiler.workspace.Workspace(pipeline, module, image_set, object_set,
m, image_set_list)
return workspace, module
def test_04_02_dont_load_file(self):
csv_text = '''"Image_FileName_DNA","Image_PathName_DNA"
"%s","%s"
''' % (self.test_filename, self.test_path)
pipeline, module, filename = self.make_pipeline(csv_text)
c0_ran = [False]
def callback(workspace):
imgset = workspace.image_set
self.assertEqual(len(imgset.names), 0)
c0_ran[0] = True
c0 = C0()
c0.callback = callback
c0.module_num = 1
pipeline.add_module(c0)
try:
module.wants_images.value = False
pipeline.run()
self.assertTrue(c0_ran[0])
finally:
os.remove(filename)
def make_workspace(self, file_names):
module = cellprofiler.modules.loadsingleimage.LoadSingleImage()
module.module_num = 1
module.directory.set_dir_choice(cellprofiler.modules.loadsingleimage.DEFAULT_INPUT_FOLDER_NAME)
for i, file_name in enumerate(file_names):
if i > 0:
module.add_file()
module.file_settings[i].image_name.value = self.get_image_name(i)
module.file_settings[i].file_name.value = file_name
pipeline = cellprofiler.pipeline.Pipeline()
def callback(caller, event):
self.assertFalse(isinstance(event, cellprofiler.pipeline.RunExceptionEvent))
pipeline.add_listener(callback)
pipeline.add_module(module)
image_set_list = cellprofiler.image.ImageSetList()
workspace = cellprofiler.workspace.Workspace(pipeline, module,
image_set_list.get_image_set(0),
cellprofiler.object.ObjectSet(), cellprofiler.measurement.Measurements(),
image_set_list)
return workspace, module
def make_pipeline(self, csv_text, name=None):
if name is None:
handle, name = tempfile.mkstemp(".csv")
fd = os.fdopen(handle, 'w')
else:
fd = open(name, "w")
fd.write(csv_text)
fd.close()
csv_path, csv_file = os.path.split(name)
module = cellprofiler.modules.loaddata.LoadText()
module.csv_directory.dir_choice = cellprofiler.setting.ABSOLUTE_FOLDER_NAME
module.csv_directory.custom_path = csv_path
module.csv_file_name.value = csv_file
module.module_num = 1
pipeline = cellprofiler.pipeline.Pipeline()
pipeline.add_module(module)
def error_callback(caller, event):
self.assertFalse(isinstance(event, cellprofiler.pipeline.RunExceptionEvent))
pipeline.add_listener(error_callback)
return pipeline, module, name
def test_03_02_zernikes_are_different(self):
'''Regression test of IMG-773'''
numpy.random.seed(32)
labels = numpy.zeros((40, 20), int)
#
# Make two "objects" composed of random foreground/background
#
labels[1:19, 1:19] = (numpy.random.uniform(size=(18, 18)) > .5).astype(int)
labels[21:39, 1:19] = (numpy.random.uniform(size=(18, 18)) > .5).astype(int) * 2
objects = cellprofiler.object.Objects()
objects.segmented = labels
object_set = cellprofiler.object.ObjectSet()
object_set.add_objects(objects, "SomeObjects")
module = cellprofiler.modules.measureobjectsizeshape.MeasureObjectAreaShape()
module.object_groups[0].name.value = "SomeObjects"
module.calculate_zernikes.value = True
module.module_num = 1
image_set_list = cellprofiler.image.ImageSetList()
measurements = cellprofiler.measurement.Measurements()
pipeline = cellprofiler.pipeline.Pipeline()
pipeline.add_module(module)
def callback(caller, event):
self.assertFalse(isinstance(event, cellprofiler.pipeline.RunExceptionEvent))
pipeline.add_listener(callback)
workspace = cellprofiler.workspace.Workspace(pipeline, module,
image_set_list.get_image_set(0),
object_set, measurements, image_set_list)
module.run(workspace)
features = [x[1] for x in module.get_measurement_columns(pipeline)
if x[0] == "SomeObjects" and
x[1].startswith("AreaShape_Zernike")]
for feature in features:
values = measurements.get_current_measurement(
"SomeObjects", feature)
self.assertEqual(len(values), 2)
self.assertNotEqual(values[0], values[1])
def test_01_03_combine_channels():
numpy.random.seed(13)
image = numpy.random.uniform(size=(20, 10, 5))
image_set_list = cellprofiler.image.ImageSetList()
image_set = image_set_list.get_image_set(0)
image_set.add(IMAGE_NAME, cellprofiler.image.Image(image))
module = cellprofiler.modules.colortogray.ColorToGray()
module.module_num = 1
module.image_name.value = IMAGE_NAME
module.combine_or_split.value = cellprofiler.modules.colortogray.COMBINE
module.grayscale_name.value = OUTPUT_IMAGE_F % 1
module.rgb_or_channels.value = cellprofiler.modules.colortogray.CH_CHANNELS
module.add_channel()
module.add_channel()
channel_indexes = numpy.array([2, 0, 3])
factors = numpy.random.uniform(size=3)
divisor = numpy.sum(factors)
expected = numpy.zeros((20, 10))
for i, channel_index in enumerate(channel_indexes):
module.channels[i].channel_choice.value = module.channel_names[channel_index]
module.channels[i].contribution.value_text = "%.10f" % factors[i]
expected += image[:, :, channel_index] * factors[i] / divisor
pipeline = cellprofiler.pipeline.Pipeline()
def callback(caller, event):
assert not isinstance(event, cellprofiler.pipeline.RunExceptionEvent)
pipeline.add_listener(callback)
pipeline.add_module(module)
workspace = Workspace(pipeline, module, image_set, cellprofiler.object.ObjectSet(),
cellprofiler.measurement.Measurements(), image_set_list)
module.run(workspace)
pixels = image_set.get_image(module.grayscale_name.value).pixel_data
assert pixels.ndim == 2
assert tuple(pixels.shape) == (20, 10)
numpy.testing.assert_almost_equal(expected, pixels)
def make_workspace(self, input_image, input_mask=None,
reference_image=None, reference_mask=None,
measurement=None):
pipeline = cellprofiler.pipeline.Pipeline()
def callback(caller, event):
self.assertFalse(isinstance(event, cellprofiler.pipeline.RunExceptionEvent))
pipeline.add_listener(callback)
object_set = cellprofiler.object.ObjectSet()
image_set_list = cellprofiler.image.ImageSetList()
image_set = image_set_list.get_image_set(0)
measurements = cellprofiler.measurement.Measurements()
module_number = 1
module = cellprofiler.modules.rescaleintensity.RescaleIntensity()
module.x_name.value = INPUT_NAME
if isinstance(input_image, (tuple, list)):
first_input_image = input_image[0]
else:
first_input_image = input_image
if isinstance(input_mask, (tuple, list)):
first_input_mask = input_mask[0]
else:
first_input_mask = input_mask
if first_input_mask is None:
image = cellprofiler.image.Image(first_input_image)
else:
image = cellprofiler.image.Image(first_input_image, first_input_mask)
ii = cellprofiler.modules.injectimage.InjectImage(INPUT_NAME, input_image, input_mask)
ii.module_num = module_number
module_number += 1
pipeline.add_module(ii)
image_set.add(INPUT_NAME, image)
module.y_name.value = OUTPUT_NAME
if reference_image is not None:
module.matching_image_name.value = REFERENCE_NAME
if reference_mask is None:
image = cellprofiler.image.Image(reference_image)
else:
image = cellprofiler.image.Image(reference_image, mask=reference_mask)
image_set.add(REFERENCE_NAME, image)
ii = cellprofiler.modules.injectimage.InjectImage(REFERENCE_NAME, reference_image, reference_mask)
ii.module_num = module_number
module_number += 1
pipeline.add_module(ii)
module.module_num = module_number
pipeline.add_module(module)
if measurement is not None:
module.divisor_measurement.value = MEASUREMENT_NAME
measurements.add_image_measurement(MEASUREMENT_NAME, measurement)
workspace = cellprofiler.workspace.Workspace(pipeline,
module,
image_set,
object_set,
measurements,
image_set_list)
return workspace, module
def test_01_04_split_channels():
numpy.random.seed(13)
image = numpy.random.uniform(size=(20, 10, 5))
image_set_list = cellprofiler.image.ImageSetList()
image_set = image_set_list.get_image_set(0)
image_set.add(IMAGE_NAME, cellprofiler.image.Image(image))
module = cellprofiler.modules.colortogray.ColorToGray()
module.module_num = 1
module.image_name.value = IMAGE_NAME
module.combine_or_split.value = cellprofiler.modules.colortogray.SPLIT
module.rgb_or_channels.value = cellprofiler.modules.colortogray.CH_CHANNELS
module.add_channel()
module.add_channel()
module.add_channel()
module.add_channel()
channel_indexes = numpy.array([1, 4, 2])
for i, channel_index in enumerate(channel_indexes):
module.channels[i].channel_choice.value = module.channel_names[channel_index]
module.channels[i].image_name.value = OUTPUT_IMAGE_F % i
pipeline = cellprofiler.pipeline.Pipeline()
def callback(caller, event):
assert not isinstance(event, cellprofiler.pipeline.RunExceptionEvent)
pipeline.add_listener(callback)
pipeline.add_module(module)
workspace = Workspace(pipeline, module, image_set, cellprofiler.object.ObjectSet(),
cellprofiler.measurement.Measurements(), image_set_list)
module.run(workspace)
for i, channel_index in enumerate(channel_indexes):
pixels = image_set.get_image(module.channels[i].image_name.value).pixel_data
assert pixels.ndim == 2
assert tuple(pixels.shape) == (20, 10)
numpy.testing.assert_almost_equal(image[:, :, channel_index], pixels)
def make_workspace(self, pixel_data, mask=None, objects=None, dimensions=2):
image_set_list = cellprofiler.image.ImageSetList()
image_set = image_set_list.get_image_set(0)
object_set = cellprofiler.object.ObjectSet()
image = cellprofiler.image.Image(pixel_data, dimensions=dimensions)
if not mask is None:
image.mask = mask
image_set.add(IMAGES_NAME, image)
if not objects is None:
o = cellprofiler.object.Objects()
o.segmented = objects
object_set.add_objects(o, OBJECTS_NAME)
module = M.MeasureImageQuality()
module.images_choice.value = M.O_SELECT
module.image_groups[0].include_image_scalings.value = False
module.image_groups[0].image_names.value = IMAGES_NAME
module.image_groups[0].use_all_threshold_methods.value = False
module.module_num = 1
pipeline = cellprofiler.pipeline.Pipeline()
pipeline.add_module(module)
workspace = cellprofiler.workspace.Workspace(pipeline, module, image_set,
object_set,
cellprofiler.measurement.Measurements(), image_set_list)
return workspace
