def test_init_bad_parameters(self):
'''test init with bad parameters'''
with self.assertRaises(Exception):
object_creator.ObjectCreator('ini_file', 'not_a_project',
'obj.xml', 'obj_alias', 'sub_alias',
'center 42', 'title')
with self.assertRaises(Exception):
# missing project_description
object_creator.ObjectCreator('ini_file', 'project', 'obj.xml',
'obj_alias', 'sub_alias', 'center 42',
'title')
with self.assertRaises(Exception):
# missing taxon_id
object_creator.ObjectCreator('ini_file', 'sample', 'obj.xml',
'obj_alias', 'sub_alias', 'center 42',
'title')
with self.assertRaises(Exception):
# missing study_accession, sample_accession, library_name, platform, instrument
object_creator.ObjectCreator('ini_file', 'experiment', 'obj.xml',
'obj_alias', 'sub_alias', 'center 42',
'title')
with self.assertRaises(Exception):
# missing experiment_accession, reads_1, md5_1, reads_2, md5_2
object_creator.ObjectCreator('ini_file', 'run', 'obj.xml',
'obj_alias', 'sub_alias', 'center 42',
'title')
def _submit_study_object(self, data_in):
if not os.path.exists(self.project_xml_dir):
os.mkdir(self.project_xml_dir)
study_accessions_from_db = {x["ena_study_accession"] for x in data_in}
if len(study_accessions_from_db) > 1:
raise Error(
"Error! More than one study ID found for dataset "
+ self.dataset_name
+ ". Got: "
+ str(study_accessions_from_db)
)
if not os.path.exists(self.project_xml):
assert study_accessions_from_db == {None}
project_alias = "project." + self.dataset_name
submit_alias = "submit." + project_alias
center_name = DatasetSubmitter._ena_center_name_from_db_data(
data_in, number_to_name_dict=self.centre_number_to_name
)
title = self.study_prefix + ". " + center_name + ". " + self.dataset_name
project_description = title
project_creator = object_creator.ObjectCreator(
self.ini_file,
"project",
self.project_xml,
project_alias,
submit_alias,
center_name,
title,
project_description,
use_test_server=self.use_test_server,
unit_test=self.unit_test,
broker_name=self.broker_name,
)
project_creator.run()
if not project_creator.submission_receipt.successful:
raise Error(
"Error submitting project to ena. XML file: " + self.project_xml
)
ena_study_accession = project_creator.submission_receipt.accessions.get(
"PROJECT", None
)
if ena_study_accession is None:
raise Error(
"Error getting proejct accession from "
+ project_creator.receipt_xml
)
for row in data_in:
row["ena_study_accession"] = ena_study_accession
self.db.update_row(
"Sample",
{"sample_id": row["sample_id"]},
{"ena_study_accession": ena_study_accession},
)
self.db.commit()
else:
assert len(study_accessions_from_db) == 1
def _submit_sample_objects(self, data_in):
submitted_samples = {} # sample id -> ena accession
for row in data_in:
if row["ena_sample_accession"] is not None:
continue
elif row["sample_id"] in submitted_samples:
row["ena_sample_accession"] = submitted_samples[row["sample_id"]]
else:
assert row["ena_sample_accession"] is None
iso_dir = isolate_dir.IsolateDir(
self.pipeline_root, row["sample_id"], row["isolate_id"]
)
object_xml = iso_dir.xml_submission_file("sample")
object_alias = "sample." + str(row["sample_id"])
submit_alias = "submit." + object_alias
center_name = DatasetSubmitter._ena_center_name_from_db_data(
data_in, number_to_name_dict=self.centre_number_to_name
)
title = (
row["subject_id"]
+ ". "
+ center_name
+ ". "
+ row["sample_id_from_lab"]
)
obj_creator = object_creator.ObjectCreator(
self.ini_file,
"sample",
object_xml,
object_alias,
submit_alias,
center_name,
title,
taxon_id=self.taxon_id,
use_test_server=self.use_test_server,
unit_test=self.unit_test,
broker_name=self.broker_name,
)
obj_creator.run()
if obj_creator.submission_receipt.successful:
try:
sample_accession = obj_creator.submission_receipt.accessions[
"SAMPLE"
]
except:
sample_accession = "FAIL"
else:
sample_accession = "FAIL"
row["ena_sample_accession"] = sample_accession
self.db.update_row(
"Sample",
{"sample_id": row["sample_id"]},
{"ena_sample_accession": sample_accession},
)
self.db.commit()
submitted_samples[row["sample_id"]] = sample_accession
def _submit_experiment_objects(self, data_in):
submitted_isolates = {} # isolate id -> ena accession
for row in data_in:
if row['ena_experiment_accession'] is not None or row[
'ena_sample_accession'] == 'FAIL':
continue
elif row['isolate_id'] in submitted_isolates:
row['ena_experiment_accession'] = submitted_isolates[
row['isolate_id']]
else:
assert row['ena_experiment_accession'] is None
iso_dir = isolate_dir.IsolateDir(self.pipeline_root,
row['sample_id'],
row['isolate_id'])
object_xml = iso_dir.xml_submission_file('experiment')
object_alias = 'experiment.' + str(row['isolate_id'])
submit_alias = 'submit.' + object_alias
center_name = DatasetSubmitter._ena_center_name_from_db_data(
data_in, number_to_name_dict=self.centre_number_to_name)
title = row['subject_id'] + '. ' + center_name + '. ' + row[
'sample_id_from_lab'] + '. ' + row[
'isolate_number_from_lab']
library_name = title
obj_creator = object_creator.ObjectCreator(
self.ini_file,
'experiment',
object_xml,
object_alias,
submit_alias,
center_name,
title,
study_accession=row['ena_study_accession'],
sample_accession=row['ena_sample_accession'],
library_name=library_name,
platform='ILLUMINA',
instrument=row['instrument_model'],
use_test_server=self.use_test_server,
unit_test=self.unit_test,
broker_name=self.broker_name,
)
obj_creator.run()
if obj_creator.submission_receipt.successful:
try:
experiment_accession = obj_creator.submission_receipt.accessions[
'EXPERIMENT']
except:
experiment_accession = 'FAIL'
else:
experiment_accession = 'FAIL'
row['ena_experiment_accession'] = experiment_accession
self.db.update_row(
'Isolate', {'isolate_id': row['isolate_id']},
{'ena_experiment_accession': experiment_accession})
self.db.commit()
submitted_isolates[row['isolate_id']] = experiment_accession
def test_run_run(self):
'''test run making run'''
obj_xml = 'tmp.object_creator.run.obj.xml'
ini_file = os.path.join(data_dir, 'conf.ini')
obj = object_creator.ObjectCreator(ini_file, 'run', obj_xml, 'object alias', 'sub alias', 'center 42', 'title', experiment_accession='ERX123', reads_1='reads1.fq', md5_1='md51', reads_2='reads2.fq', md5_2='md52', unit_test='success')
obj.run()
self.assertTrue(obj.submission_receipt.successful)
os.unlink(obj_xml)
self.assertTrue(os.path.exists(obj.submission_xml))
os.unlink(obj.submission_xml)
self.assertTrue(os.path.exists(obj.receipt_xml))
os.unlink(obj.receipt_xml)
def test_run_experiment(self):
'''test run making experiment'''
obj_xml = 'tmp.object_creator.experiment.obj.xml'
ini_file = os.path.join(data_dir, 'conf.ini')
obj = object_creator.ObjectCreator(ini_file, 'experiment', obj_xml, 'objct alias', 'sub alias', 'center 42', 'title', study_accession='ERP123', sample_accession='ERS42', library_name='lib name', platform='ILLUMINA', instrument='HISEQ', unit_test='success')
obj.run()
self.assertTrue(obj.submission_receipt.successful)
os.unlink(obj_xml)
self.assertTrue(os.path.exists(obj.submission_xml))
os.unlink(obj.submission_xml)
self.assertTrue(os.path.exists(obj.receipt_xml))
os.unlink(obj.receipt_xml)
def test_run_sample(self):
'''test run making sample'''
obj_xml = 'tmp.object_creator.sample.obj.xml'
ini_file = os.path.join(data_dir, 'conf.ini')
obj = object_creator.ObjectCreator(ini_file, 'sample', obj_xml, 'objct alias', 'sub alias', 'center 42', 'title', taxon_id=42, unit_test='success')
obj.run()
self.assertTrue(obj.submission_receipt.successful)
os.unlink(obj_xml)
self.assertTrue(os.path.exists(obj.submission_xml))
os.unlink(obj.submission_xml)
self.assertTrue(os.path.exists(obj.receipt_xml))
os.unlink(obj.receipt_xml)
def test_run_project(self):
'''test run making project'''
obj_xml = 'tmp.object_creator.project.obj.xml'
ini_file = os.path.join(data_dir, 'conf.ini')
obj = object_creator.ObjectCreator(ini_file, 'project', obj_xml, 'objct alias', 'sub alias', 'center 42', 'title', project_description='project description', unit_test='success')
obj.run()
self.assertTrue(obj.submission_receipt.successful)
os.unlink(obj_xml)
self.assertTrue(os.path.exists(obj.submission_xml))
os.unlink(obj.submission_xml)
self.assertTrue(os.path.exists(obj.receipt_xml))
os.unlink(obj.receipt_xml)
def _submit_study_object(self, data_in):
if not os.path.exists(self.project_xml_dir):
os.mkdir(self.project_xml_dir)
study_accessions_from_db = {x['ena_study_accession'] for x in data_in}
if len(study_accessions_from_db) > 1:
raise Error('Error! More than one study ID found for dataset ' +
self.dataset_name + '. Got: ' +
str(study_accessions_from_db))
if not os.path.exists(self.project_xml):
assert study_accessions_from_db == {None}
project_alias = 'project.' + self.dataset_name
submit_alias = 'submit.' + project_alias
center_name = DatasetSubmitter._ena_center_name_from_db_data(
data_in, number_to_name_dict=self.centre_number_to_name)
title = self.study_prefix + '. ' + center_name + '. ' + self.dataset_name
project_description = title
project_creator = object_creator.ObjectCreator(
self.ini_file,
'project',
self.project_xml,
project_alias,
submit_alias,
center_name,
title,
project_description,
use_test_server=self.use_test_server,
unit_test=self.unit_test,
broker_name=self.broker_name)
project_creator.run()
if not project_creator.submission_receipt.successful:
raise Error('Error submitting project to ena. XML file: ' +
self.project_xml)
ena_study_accession = project_creator.submission_receipt.accessions.get(
'PROJECT', None)
if ena_study_accession is None:
raise Error('Error getting proejct accession from ' +
project_creator.receipt_xml)
for row in data_in:
row['ena_study_accession'] = ena_study_accession
self.db.update_row(
'Sample', {'sample_id': row['sample_id']},
{'ena_study_accession': ena_study_accession})
self.db.commit()
else:
assert len(study_accessions_from_db) == 1
def _submit_sample_objects(self, data_in):
submitted_samples = {} # sample id -> ena accession
for row in data_in:
if row['ena_sample_accession'] is not None:
continue
elif row['sample_id'] in submitted_samples:
row['ena_sample_accession'] = submitted_samples[
row['sample_id']]
else:
assert row['ena_sample_accession'] is None
iso_dir = isolate_dir.IsolateDir(self.pipeline_root,
row['sample_id'],
row['isolate_id'])
object_xml = iso_dir.xml_submission_file('sample')
object_alias = 'sample.' + str(row['sample_id'])
submit_alias = 'submit.' + object_alias
center_name = DatasetSubmitter._ena_center_name_from_db_data(
data_in, number_to_name_dict=self.centre_number_to_name)
title = row['subject_id'] + '. ' + center_name + '. ' + row[
'sample_id_from_lab']
obj_creator = object_creator.ObjectCreator(
self.ini_file,
'sample',
object_xml,
object_alias,
submit_alias,
center_name,
title,
taxon_id=self.taxon_id,
use_test_server=self.use_test_server,
unit_test=self.unit_test,
broker_name=self.broker_name)
obj_creator.run()
if obj_creator.submission_receipt.successful:
try:
sample_accession = obj_creator.submission_receipt.accessions[
'SAMPLE']
except:
sample_accession = 'FAIL'
else:
sample_accession = 'FAIL'
row['ena_sample_accession'] = sample_accession
self.db.update_row('Sample', {'sample_id': row['sample_id']},
{'ena_sample_accession': sample_accession})
self.db.commit()
submitted_samples[row['sample_id']] = sample_accession
def _submit_runs(self, data_in):
# Note: reads have to be in the dropbox before submitting the Run object.
# Upload all the reads first, in parallel, then submit the runs.
fq_pairs_to_upload = [
] # (seqrep, full path on disk1, dropbox name1, full path on disk2, dropbox name2)
for row in data_in:
iso_dir = isolate_dir.IsolateDir(self.pipeline_root,
row['sample_id'],
row['isolate_id'])
fq_pairs_to_upload.append((
row['seqrep_id'],
iso_dir.reads_filename('remove_contam',
row['sequence_replicate_number'], 1),
str(row['seqrep_id']) + '.1.' +
row['remove_contam_reads_file_1_md5'] + '.fq.gz',
iso_dir.reads_filename('remove_contam',
row['sequence_replicate_number'], 2),
str(row['seqrep_id']) + '.2.' +
row['remove_contam_reads_file_2_md5'] + '.fq.gz',
))
self.pool = multiprocessing.Pool(self.fq_upload_threads)
upload_return_values = self.pool.starmap(
_upload_fastq_file_pair,
zip(fq_pairs_to_upload, itertools.repeat(self.ini_file),
itertools.repeat(self.unit_test)))
upload_success = {x[0]: x[1] for x in upload_return_values}
fq_pairs_to_upload = {x[0]: x for x in fq_pairs_to_upload}
# Fastqs are uploaded, now submit the xmls and update the database
for row in data_in:
assert row['seqrep_id'] in fq_pairs_to_upload
assert row['seqrep_id'] in upload_success
assert row['ena_run_accession'] is None
assert row['ena_experiment_accession'] is not None
iso_dir = isolate_dir.IsolateDir(self.pipeline_root,
row['sample_id'],
row['isolate_id'])
object_xml = iso_dir.xml_submission_file(
'run', sequence_replicate=row['sequence_replicate_number'])
object_alias = 'run.' + str(row['isolate_id'])
submit_alias = 'submit.' + object_alias
center_name = DatasetSubmitter._ena_center_name_from_db_data(
data_in, number_to_name_dict=self.centre_number_to_name)
title = None # not needed for a run
obj_creator = object_creator.ObjectCreator(
self.ini_file,
'run',
object_xml,
object_alias,
submit_alias,
center_name,
title,
experiment_accession=row['ena_experiment_accession'],
reads_1=fq_pairs_to_upload[row['seqrep_id']][2],
md5_1=row['remove_contam_reads_file_1_md5'],
reads_2=fq_pairs_to_upload[row['seqrep_id']][4],
md5_2=row['remove_contam_reads_file_2_md5'],
use_test_server=self.use_test_server,
unit_test=self.unit_test,
broker_name=self.broker_name,
)
obj_creator.run()
if obj_creator.submission_receipt.successful:
try:
run_accession = obj_creator.submission_receipt.accessions[
'RUN']
except:
run_accession = 'FAIL'
else:
run_accession = 'FAIL'
row['ena_run_accession'] = run_accession
self.db.update_row('Seqrep', {'seqrep_id': row['seqrep_id']},
{'ena_run_accession': run_accession})
self.db.commit()
def _submit_experiment_objects(self, data_in):
submitted_isolates = {} # isolate id -> ena accession
for row in data_in:
if (
row["ena_experiment_accession"] is not None
or row["ena_sample_accession"] == "FAIL"
):
continue
elif row["isolate_id"] in submitted_isolates:
row["ena_experiment_accession"] = submitted_isolates[row["isolate_id"]]
else:
assert row["ena_experiment_accession"] is None
iso_dir = isolate_dir.IsolateDir(
self.pipeline_root, row["sample_id"], row["isolate_id"]
)
object_xml = iso_dir.xml_submission_file("experiment")
object_alias = "experiment." + str(row["isolate_id"])
submit_alias = "submit." + object_alias
center_name = DatasetSubmitter._ena_center_name_from_db_data(
data_in, number_to_name_dict=self.centre_number_to_name
)
title = (
row["subject_id"]
+ ". "
+ center_name
+ ". "
+ row["sample_id_from_lab"]
+ ". "
+ row["isolate_number_from_lab"]
)
library_name = title
obj_creator = object_creator.ObjectCreator(
self.ini_file,
"experiment",
object_xml,
object_alias,
submit_alias,
center_name,
title,
study_accession=row["ena_study_accession"],
sample_accession=row["ena_sample_accession"],
library_name=library_name,
platform="ILLUMINA",
instrument=row["instrument_model"],
use_test_server=self.use_test_server,
unit_test=self.unit_test,
broker_name=self.broker_name,
)
obj_creator.run()
if obj_creator.submission_receipt.successful:
try:
experiment_accession = obj_creator.submission_receipt.accessions[
"EXPERIMENT"
]
except:
experiment_accession = "FAIL"
else:
experiment_accession = "FAIL"
row["ena_experiment_accession"] = experiment_accession
self.db.update_row(
"Isolate",
{"isolate_id": row["isolate_id"]},
{"ena_experiment_accession": experiment_accession},
)
self.db.commit()
submitted_isolates[row["isolate_id"]] = experiment_accession
