def make_halo(search_distances, scan_distances, ratios=None):
"""returns the organism object to work on"""
keggfile = util.read_dfile(KEGG_FILE_PATH, comment='#')
gofile = util.read_dfile(GO_FILE_PATH)
rsatdb = rsat.RsatDatabase(RSAT_BASE_URL, CACHE_DIR, 'Halobacterium sp',
64091)
mo_db = microbes_online.MicrobesOnline(CACHE_DIR)
stringfile = 'testdata/string_links_64091.tab'
nw_factories = []
if stringfile != None:
nw_factories.append(
stringdb.get_network_factory('hal', stringfile, 0.5))
else:
logging.warn("no STRING file specified !")
if ratios is not None:
nw_factories.append(
microbes_online.get_network_factory(
mo_db, max_operon_size=ratios.num_rows / 20, weight=0.5))
keggorg = util.make_dfile_map(keggfile, 1, 3)['hal']
rsat_organism = rsatdb.get_rsat_organism(keggorg)
rsat_info = org.RsatSpeciesInfo(rsatdb, keggorg, rsat_organism, 64091)
gotax = util.make_dfile_map(gofile, 0, 1)[rsat_info.go_species()]
return org.Microbe('hal', keggorg, rsat_info, gotax, mo_db, nw_factories,
search_distances, scan_distances, True, None)
def make_microbe(code):
"""assemble organism related information and return it to the caller"""
keggfile = util.read_dfile(KEGG_FILE_PATH, comment='#')
rsatdb = rsat.RsatDatabase(RSAT_BASE_URL, CACHE_DIR)
kegg_mapper = org.make_kegg_code_mapper(keggfile)
rsat_mapper = org.make_rsat_organism_mapper(rsatdb)
rsat_info = rsat_mapper(kegg_mapper(code))
microbedb = MicrobeDB(keggfile, rsatdb, rsat_info)
print "NCBI CODE IS: ", rsat_info.taxonomy_id
gofile = util.read_dfile(GO_FILE_PATH)
mo_db = microbes_online.MicrobesOnline()
search_distances = {'upstream': (-20, 150)}
scan_distances = {'upstream': (-30, 250)}
org_factory = org.MicrobeFactory(kegg_mapper, rsat_mapper,
org.make_go_taxonomy_mapper(gofile),
mo_db, [])
organism = org_factory.create(code, search_distances, scan_distances)
return microbedb, organism
def make_organism(self):
"""returns the organism object to work on"""
self.__make_dirs_if_needed()
ncbi_code, kegg_species = self.__get_kegg_data()
try:
go_file_path = resource_filename(Requirement.parse("cmonkey2"),
USER_GO_FILE_PATH)
except DistributionNotFound:
go_file_path = USER_GO_FILE_PATH
gofile = util.read_dfile(go_file_path)
if self['rsat_dir']:
if not self['rsat_organism']:
raise Exception(
'override RSAT loading: please specify --rsat_organism')
logging.info("using RSAT files for '%s'", self['rsat_organism'])
rsatdb = rsat.RsatFiles(self['rsat_dir'], self['rsat_organism'],
ncbi_code, self['rsat_features'],
self['rsat_base_url'])
else:
rsatdb = rsat.RsatDatabase(self['rsat_base_url'],
self['cache_dir'], kegg_species,
ncbi_code, self['rsat_features'])
if self['operon_file']:
logging.info("using operon file at '%s'", self['operon_file'])
mo_db = microbes_online.MicrobesOnlineOperonFile(
self['operon_file'])
else:
logging.info(
"attempting automatic download of operons from Microbes Online"
)
mo_db = microbes_online.MicrobesOnline(self['cache_dir'])
stringfile = self['string_file']
nw_factories = []
is_microbe = self['organism_code'] not in VERTEBRATES
# determine the final weights. note: for now, we will just check whether
# we have 1 or 2 networks
num_networks = 0
if not self['nonetworks'] and self['use_string']:
num_networks += 1
if is_microbe and not self['nonetworks'] and self['use_operons']:
num_networks += 1
network_weight = 0.0
if num_networks > 0:
network_weight = 1.0 / num_networks
# do we use STRING ?
if not self['nonetworks'] and self['use_string']:
# download if not provided
if stringfile is None:
if ncbi_code is None:
rsat_info = org.RsatSpeciesInfo(rsatdb, kegg_species,
self['rsat_organism'],
None)
ncbi_code = rsat_info.taxonomy_id
logging.info("NCBI CODE IS: %s", ncbi_code)
url = STRING_URL_PATTERN % ncbi_code
stringfile = "%s/%s.gz" % (self['cache_dir'], ncbi_code)
self['string_file'] = stringfile
logging.info(
"Automatically using STRING file in '%s' (URL: %s)",
stringfile, url)
util.get_url_cached(url, stringfile)
else:
logging.info("Loading STRING file at '%s'", stringfile)
# create and add network
nw_factories.append(
stringdb.get_network_factory(self['organism_code'], stringfile,
network_weight))
# do we use operons ?
if is_microbe and not self['nonetworks'] and self['use_operons']:
logging.debug('adding operon network factory')
nw_factories.append(
microbes_online.get_network_factory(
mo_db,
max_operon_size=self.ratios.num_rows / 20,
weight=network_weight))
orgcode = self['organism_code']
logging.debug("Creating Microbe object for '%s'", orgcode)
rsat_info = org.RsatSpeciesInfo(rsatdb, kegg_species,
self['rsat_organism'], ncbi_code)
gotax = util.make_dfile_map(gofile, 0, 1)[rsat_info.go_species()]
synonyms = None
if self['synonym_file'] is not None:
synonyms = thesaurus.create_from_delimited_file2(
self['synonym_file'], self['case_sensitive'])
#New logic: test to see if there's a fastafile. If not, then
#Download it from rsat, process it, and then return the new file name
is_microbe = True
if is_microbe:
organism = org.Microbe(orgcode, kegg_species, rsat_info, gotax,
mo_db, nw_factories,
self['search_distances'],
self['scan_distances'], self['use_operons'],
self.ratios, synonyms, self['fasta_file'])
else:
organism = org.RSATOrganism(orgcode, kegg_species, rsat_info,
gotax, nw_factories,
self['search_distances'],
self['scan_distances'], self.ratios,
synonyms, self['fasta_file'])
conn = self.__dbconn()
with conn:
for network in organism.networks():
conn.execute("insert into statstypes values ('network',?)",
[network.name])
for sequence_type in self['sequence_types']:
conn.execute("insert into statstypes values ('seqtype',?)",
[sequence_type])
return organism
def setUp(self): # pylint: disable-msg=C0103
"""test fixture"""
if not os.path.exists('testcache'):
os.mkdir('testcache')
self.service = mo.MicrobesOnline('testcache',
mo.MICROBES_ONLINE_BASE_URL)