def setUp(self):
super().setUp()
self.op = flow.PCAOp(name = "PCA",
channels = ["V2-A", "V2-H", "Y2-A", "Y2-H"],
scale = {"V2-A" : "log",
"V2-H" : "log",
"Y2-A" : "log",
"Y2-H" : "log"},
num_components = 2)
def setUp(self):
self.cwd = os.path.dirname(os.path.abspath(__file__)) + "/data/Plate01/"
tube1 = flow.Tube(file = self.cwd + 'RFP_Well_A3.fcs', conditions = {"Dox" : 10.0})
tube2 = flow.Tube(file= self.cwd + 'CFP_Well_A4.fcs', conditions = {"Dox" : 1.0})
import_op = flow.ImportOp(conditions = {"Dox" : "float"},
tubes = [tube1, tube2])
self.ex = import_op.apply()
self.op = flow.PCAOp(name = "PCA",
channels = ["V2-A", "V2-H", "Y2-A", "Y2-H"],
scale = {"V2-A" : "log",
"V2-H" : "log",
"Y2-A" : "log",
"Y2-H" : "log"},
num_components = 2)