def setUp(self):
self.cwd = os.path.dirname(os.path.abspath(__file__)) + "/data/Plate01/"
tube1 = flow.Tube(file = self.cwd + 'RFP_Well_A3.fcs', conditions = {"Dox" : 10.0})
tube2 = flow.Tube(file= self.cwd + 'CFP_Well_A4.fcs', conditions = {"Dox" : 1.0})
import_op = flow.ImportOp(conditions = {"Dox" : "float"},
tubes = [tube1, tube2])
self.ex = import_op.apply()
self.gate = flow.RangeOp(name = "Range",
channel = "Y2-A",
low = 1000,
high = 10000)
def setUp(self):
self.cwd = os.path.dirname(
os.path.abspath(__file__)) + "/data/Plate01/"
tube1 = flow.Tube(file=self.cwd + 'RFP_Well_A3.fcs',
conditions={"Dox": 10.0})
tube2 = flow.Tube(file=self.cwd + 'CFP_Well_A4.fcs',
conditions={"Dox": 1.0})
import_op = flow.ImportOp(conditions={"Dox": "float"},
tubes=[tube1, tube2])
ex = import_op.apply()
# this works so much better on transformed data
logicle = flow.LogicleTransformOp()
logicle.name = "Logicle transformation"
logicle.channels = ['V2-A', 'Y2-A', 'B1-A']
logicle.estimate(ex)
self.ex = logicle.apply(ex)
self.gate = flow.RangeOp(name="Range",
channel="Y2-A",
low=0.4,
high=0.8)
useblit=True)
else:
self._span = None
def _onselect(self, xmin, xmax):
"""Update selection traits"""
self.op.low = xmin
self.op.high = xmax
util.expand_class_attributes(RangeSelection)
util.expand_method_parameters(RangeSelection, RangeSelection.plot)
if __name__ == '__main__':
import cytoflow as flow
tube1 = flow.Tube(file='../../cytoflow/tests/data/Plate01/RFP_Well_A3.fcs',
conditions={"Dox": 10.0})
tube2 = flow.Tube(file='../../cytoflow/tests/data/Plate01/CFP_Well_A4.fcs',
conditions={"Dox": 1.0})
ex = flow.ImportOp(conditions={"Dox": "float"}, tubes=[tube1, tube2])
r = flow.RangeOp(channel='Y2-A')
rv = r.default_view(scale="logicle")
plt.ioff()
rv.plot(ex)
rv.interactive = True
plt.show()
def setUp(self):
super().setUp()
self.gate = flow.RangeOp(name="Range",
channel="Y2-A",
low=1000,
high=10000)