def test_gilda_disambiguation():
gm.gilda_mode = 'web'
er1 = Agent('NDR1', db_refs={'TEXT': 'NDR1'})
pmid1 = '18362890'
stmt1 = Phosphorylation(None, er1,
evidence=[Evidence(pmid=pmid1,
text_refs={'PMID': pmid1})])
er2 = Agent('NDR1', db_refs={'TEXT': 'NDR1'})
pmid2 = '16832411'
stmt2 = Inhibition(None, er2,
evidence=[Evidence(pmid=pmid2,
text_refs={'PMID': pmid2})])
mapped_stmts1 = gm.map_stmts([stmt1])
assert mapped_stmts1[0].sub.name == 'STK38', mapped_stmts1[0].sub.name
assert mapped_stmts1[0].sub.db_refs['HGNC'] == '17847', \
mapped_stmts1[0].sub.db_refs
assert mapped_stmts1[0].sub.db_refs['UP'] == 'Q15208', \
mapped_stmts1[0].sub.db_refs
mapped_stmts2 = gm.map_stmts([stmt2])
assert mapped_stmts2[0].obj.name == 'NDRG1', \
mapped_stmts2[0].obj.name
assert mapped_stmts2[0].obj.db_refs['HGNC'] == '7679', \
mapped_stmts2[0].obj.db_refs
assert mapped_stmts2[0].obj.db_refs['UP'] == 'Q92597', \
mapped_stmts2[0].obj.db_refs
annotations = mapped_stmts2[0].evidence[0].annotations
assert len(annotations['agents']['gilda'][1]) == 2, \
annotations
assert annotations['agents']['gilda'][0] is None
assert annotations['agents']['gilda'][1] is not None
def test_adeft_mapping_non_pos():
er = Agent('ER', db_refs={'TEXT': 'ER'})
# This is an exact definition of a pos_label entry so we
# expect that it will be applied as a grounding even though the
# Adeft model has low precision for this label.
ev = Evidence(text='estradiol (ER)')
stmt = Phosphorylation(None, er, evidence=[ev])
mapped_stmt = gm.map_stmts([stmt])[0]
assert 'CHEBI' in mapped_stmt.sub.db_refs, mapped_stmt.evidence
# This one is not an exact definition so we expect the grounding to
# be stripped out.
ev = Evidence(text='Estradiol is one of the three estrogen hormones'
'naturally produced in the body.')
stmt = Phosphorylation(None, er, evidence=[ev])
mapped_stmt = gm.map_stmts([stmt])[0]
assert 'CHEBI' not in mapped_stmt.sub.db_refs, mapped_stmt.evidence
# This is a non-positive label, and we expect it to be stripped out
# whether it's an exact definition or not.
pcs = Agent('PCS', db_refs={'TEXT': 'PCS', 'MESH': 'xxx'})
ev = Evidence(text='physical component summary (PCS)')
stmt = Phosphorylation(None, pcs, evidence=[ev])
mapped_stmt = gm.map_stmts([stmt])[0]
assert 'MESH' not in mapped_stmt.sub.db_refs, \
(mapped_stmt.sub.db_refs, mapped_stmt.evidence)
ev = Evidence(text='physical component summary')
stmt = Phosphorylation(None, pcs, evidence=[ev])
mapped_stmt = gm.map_stmts([stmt])[0]
assert 'MESH' not in mapped_stmt.sub.db_refs, \
(mapped_stmt.sub.db_refs, mapped_stmt.evidence)
def test_adeft_mapping():
er1 = Agent('ER', db_refs={'TEXT': 'ER'})
pmid1 = '30775882'
stmt1 = Phosphorylation(None, er1, evidence=[Evidence(pmid=pmid1,
text_refs={'PMID':
pmid1})])
er2 = Agent('ER', db_refs={'TEXT': 'ER'})
pmid2 = '28369137'
stmt2 = Inhibition(None, er2, evidence=[Evidence(pmid=pmid2,
text_refs={'PMID':
pmid2})])
mapped_stmts1 = gm.map_stmts([stmt1])
assert mapped_stmts1[0].sub.name == 'ESR', \
mapped_stmts1[0].sub.name
assert mapped_stmts1[0].sub.db_refs['FPLX'] == 'ESR', \
mapped_stmts1[0].sub.db_refs
mapped_stmts2 = gm.map_stmts([stmt2])
assert mapped_stmts2[0].obj.name == 'endoplasmic reticulum', \
mapped_stmts2[0].obj.name
assert mapped_stmts2[0].obj.db_refs['GO'] == 'GO:0005783', \
mapped_stmts2[0].obj.db_refs
annotations = mapped_stmts2[0].evidence[0].annotations
assert 'GO:GO:0005783' in annotations['agents']['adeft'][1]
def test_adeft_mapping_non_pos():
pcs = Agent('PCS', db_refs={'TEXT': 'PCS'})
# This is an exact definition of a non-positive label entry so we
# expect that it will be applied as a grounding
ev = Evidence(text='post concussive symptoms (PCS)')
stmt = Phosphorylation(None, pcs, evidence=[ev])
mapped_stmt = gm.map_stmts([stmt])[0]
assert 'MESH' in mapped_stmt.sub.db_refs, mapped_stmt.evidence
pcs = Agent('PCS', db_refs={'TEXT': 'PCS', 'MESH': 'xxx'})
ev = Evidence(text='physical component summary')
stmt = Phosphorylation(None, pcs, evidence=[ev])
mapped_stmt = gm.map_stmts([stmt])[0]
assert 'MESH' not in mapped_stmt.sub.db_refs, \
(mapped_stmt.sub.db_refs, mapped_stmt.evidence)
def test_adeft_mapping_non_pos():
pcs = Agent('PCS', db_refs={'TEXT': 'PCS'})
# This is an exact definition of a non-positive label entry so we
# expect that it will be applied as a grounding
ev = Evidence(text='post concussive symptoms (PCS)')
stmt = Phosphorylation(None, pcs, evidence=[ev])
mapped_stmt = gm.map_stmts([stmt])[0]
assert 'MESH' in mapped_stmt.sub.db_refs, mapped_stmt.evidence
pcs = Agent('PCS', db_refs={'TEXT': 'PCS', 'MESH': 'xxx'})
# There a non-positive entry is implied but not exactly, so
# the prior grounding will be removed.
ev = Evidence(text='post symptoms concussive concussion')
stmt = Phosphorylation(None, pcs, evidence=[ev])
mapped_stmt = gm.map_stmts([stmt])[0]
assert 'MESH' not in mapped_stmt.sub.db_refs, mapped_stmt.evidence
def test_misgrounding():
baz1 = Agent('ZNF214', db_refs={'TEXT': 'baz1', 'HGNC': '13006'})
stmt = Phosphorylation(None, baz1)
stmts = gm.map_stmts([stmt])
stmt = stmts[0]
assert len(stmt.sub.db_refs) == 1, stmt.sub.db_refs
assert stmt.sub.db_refs['TEXT'] == 'baz1'
assert stmt.sub.name == 'baz1'
def test_map_agent():
erk = Agent('ERK1', db_refs={'TEXT': 'ERK1'})
p_erk = Agent('P-ERK', db_refs={'TEXT': 'p-ERK'})
stmt = Complex([erk, p_erk])
mapped_stmts = gm.map_stmts([stmt])
mapped_ag = mapped_stmts[0].members[1]
assert mapped_ag.name == 'ERK'
assert mapped_ag.db_refs.get('FPLX') == 'ERK'
def test_simple_mapping():
akt = Agent('pkbA', db_refs={'TEXT': 'Akt', 'UP': 'XXXXXX'})
stmt = Phosphorylation(None, akt)
mapped_stmts = gm.map_stmts([stmt])
assert len(mapped_stmts) == 1
mapped_akt = mapped_stmts[0].sub
assert mapped_akt.db_refs['TEXT'] == 'Akt'
assert mapped_akt.db_refs['FPLX'] == 'AKT'
def test_map_standardize_up_hgnc():
a1 = Agent('MAPK1', db_refs={'HGNC': '6871'})
a2 = Agent('MAPK1', db_refs={'UP': 'P28482'})
stmt = Phosphorylation(a1, a2)
mapped_stmts = gm.map_stmts([stmt])
assert len(mapped_stmts) == 1
st = mapped_stmts[0]
assert st.enz.db_refs['HGNC'] == st.sub.db_refs['HGNC']
assert st.enz.db_refs['UP'] == st.sub.db_refs['UP']
def test_in_place_overwrite_of_gm():
"""Make sure HGNC lookups don't modify the original grounding map by adding
keys."""
erk = Agent('ERK1', db_refs={'TEXT': 'ERK1'})
stmt = Phosphorylation(None, erk)
g_map = {'ERK1': {'TEXT': 'ERK1', 'UP': 'P28482'}}
gm = GroundingMapper(g_map)
mapped_stmts = gm.map_stmts([stmt])
gmap_after_mapping = gm.grounding_map
assert set(gmap_after_mapping['ERK1'].keys()) == set(['TEXT', 'UP'])
def test_up_and_mismatched_hgnc():
erk = Agent('ERK1', db_refs={'TEXT': 'ERK1'})
stmt = Phosphorylation(None, erk)
g_map = {'ERK1': {'TEXT': 'ERK1', 'UP': 'P28482', 'HGNC': '6877'}}
gm = GroundingMapper(g_map)
mapped_stmts = gm.map_stmts([stmt])
assert mapped_stmts[0].sub.db_refs['HGNC'] == '6877', \
mapped_stmts[0].sub.db_refs
assert mapped_stmts[0].sub.db_refs['UP'] == 'P27361', \
mapped_stmts[0].sub.db_refs
def test_up_with_no_gene_name_with_hgnc_sym():
erk = Agent('ERK1', db_refs={'TEXT': 'ERK1'})
stmt = Phosphorylation(None, erk)
g_map = {'ERK1': {'TEXT': 'ERK1', 'UP': 'A0K5Q6', 'HGNC': '6871'}}
gm = GroundingMapper(g_map)
mapped_stmts = gm.map_stmts([stmt])
assert mapped_stmts[0].sub.db_refs['HGNC'] == '6871', \
mapped_stmts[0].sub.db_refs
assert mapped_stmts[0].sub.db_refs['UP'] == 'P28482', \
mapped_stmts[0].sub.db_refs
def test_map_standardize_chebi_hmdb():
a1 = Agent('X', db_refs={'HMDB': 'HMDB0000122'})
a2 = Agent('Y', db_refs={'CHEBI': 'CHEBI:15903'})
stmt = Phosphorylation(a1, a2)
mapped_stmts = gm.map_stmts([stmt])
assert len(mapped_stmts) == 1
st = mapped_stmts[0]
assert st.enz.db_refs['CHEBI'] == st.sub.db_refs['CHEBI'], \
(st.enz.db_refs, st.sub.db_refs)
assert st.enz.name == 'beta-D-glucose', st.enz
assert st.sub.name == 'beta-D-glucose', st.sub
def test_hgnc_but_not_up():
erk = Agent('ERK1', db_refs={'TEXT': 'ERK1'})
stmt = Phosphorylation(None, erk)
g_map = {'ERK1': {'TEXT': 'ERK1', 'HGNC': '6871'}}
gm = GroundingMapper(g_map)
mapped_stmts = gm.map_stmts([stmt])
assert len(mapped_stmts) == 1
mapped_erk = mapped_stmts[0].sub
assert mapped_erk.name == 'MAPK1'
assert mapped_erk.db_refs['TEXT'] == 'ERK1'
assert mapped_erk.db_refs['HGNC'] == '6871'
assert mapped_erk.db_refs['UP'] == 'P28482'
def test_text_and_norm_text():
gm.gilda_mode = 'local'
# We should filter out ignores in both TEXT and TEXT_NORM
ag = Agent('x', db_refs={'TEXT': 'XREF_BIBR', 'TEXT_NORM': 'ERK'})
stmt = Phosphorylation(None, ag)
res = gm.map_stmts([stmt])
assert not res
ag = Agent('x', db_refs={'TEXT': 'ERK', 'TEXT_NORM': 'XREF_BIBR'})
stmt = Phosphorylation(None, ag)
res = gm.map_stmts([stmt])
assert not res
# We should disambiguate based on both TEXT and TEXT_NORM
ag = Agent('x', db_refs={'TEXT': 'AA', 'TEXT_NORM': 'XXX'},)
stmt = Phosphorylation(None, ag,
evidence=Evidence(text='Arachidonic acid (AA)'))
res = gm.map_stmts([stmt])
assert res[0].sub.name == 'arachidonic acid', res[0]
ag = Agent('x', db_refs={'TEXT': 'XXX', 'TEXT_NORM': 'AA'})
stmt = Phosphorylation(None, ag,
evidence=Evidence(text='Arachidonic acid (AA)'))
res = gm.map_stmts([stmt])
assert res[0].sub.name == 'arachidonic acid', res[0]
ag = Agent('x', db_refs={'TEXT': 'XXX', 'TEXT_NORM': 'ERK'})
stmt = Phosphorylation(None, ag)
res = gm.map_stmts([stmt])
assert res[0].sub.name == 'ERK', res[0]
ag = Agent('x', db_refs={'TEXT': 'ERK', 'TEXT_NORM': 'XXX'})
stmt = Phosphorylation(None, ag)
res = gm.map_stmts([stmt])
assert res[0].sub.name == 'ERK', res[0]
def test_map_standardize_chebi_pc():
a1 = Agent('X', db_refs={'PUBCHEM': '42611257'})
a2 = Agent('Y', db_refs={'CHEBI': 'CHEBI:63637'})
stmt = Phosphorylation(a1, a2)
mapped_stmts = gm.map_stmts([stmt])
assert len(mapped_stmts) == 1
st = mapped_stmts[0]
assert st.enz.db_refs['PUBCHEM'] == st.sub.db_refs['PUBCHEM'], \
(st.enz.db_refs, st.sub.db_refs)
assert st.enz.db_refs['CHEBI'] == st.sub.db_refs['CHEBI'], \
(st.enz.db_refs, st.sub.db_refs)
assert st.enz.name == 'vemurafenib'
assert st.sub.name == 'vemurafenib'
def test_up_id_with_no_gene_name():
"""Expect no HGNC entry; no error raised."""
no_gn = Agent('NoGNname', db_refs={'TEXT': 'NoGN'})
stmt = Phosphorylation(None, no_gn)
g_map = {'NoGN': {'TEXT': 'NoGN', 'UP': 'A0K5Q6'}}
gm = GroundingMapper(g_map)
mapped_stmts = gm.map_stmts([stmt])
assert len(mapped_stmts) == 1
mapped_ag = mapped_stmts[0].sub
assert mapped_ag.name == 'NoGNname'
assert mapped_ag.db_refs['TEXT'] == 'NoGN'
assert mapped_ag.db_refs.get('HGNC') is None
assert mapped_ag.db_refs['UP'] == 'A0K5Q6'
def test_up_id_with_no_hgnc_id():
"""Non human protein"""
gag = Agent('Gag', db_refs={'TEXT': 'Gag'})
stmt = Phosphorylation(None, gag)
g_map = {'Gag': {'TEXT': 'Gag', 'UP': 'P04585'}}
gm = GroundingMapper(g_map)
mapped_stmts = gm.map_stmts([stmt])
assert len(mapped_stmts) == 1
mapped_gag = mapped_stmts[0].sub
assert mapped_gag.name == 'gag-pol'
assert mapped_gag.db_refs['TEXT'] == 'Gag'
assert mapped_gag.db_refs.get('HGNC') is None
assert mapped_gag.db_refs['UP'] == 'P04585'
def test_map_entry_hgnc_and_up():
"""Make sure that HGNC symbol is replaced with HGNC ID when grounding map
includes both UP ID and HGNC symbol."""
rela = Agent('NF-kappaB p65', db_refs={'TEXT': 'NF-kappaB p65'})
erk = Agent('ERK1', db_refs={'TEXT': 'ERK1'})
stmt = Phosphorylation(erk, rela)
g_map = {'NF-kappaB p65': {'TEXT': 'NF-kappaB p65', 'UP': 'Q04206',
'HGNC': '9955'}}
gm = GroundingMapper(g_map)
mapped_stmts = gm.map_stmts([stmt])
assert len(mapped_stmts) == 1
ms = mapped_stmts[0]
assert ms.sub.db_refs == {'TEXT': 'NF-kappaB p65', 'UP': 'Q04206',
'HGNC': '9955'}
def test_gilda_disambiguation_local():
gm.gilda_mode = 'local'
er1 = Agent('NDR1', db_refs={'TEXT': 'NDR1'})
pmid1 = '18362890'
stmt1 = Phosphorylation(
None, er1, evidence=[Evidence(pmid=pmid1, text_refs={'PMID': pmid1})])
mapped_stmts1 = gm.map_stmts([stmt1])
annotations = mapped_stmts1[0].evidence[0].annotations
assert annotations['agents']['gilda'][0] is None
assert annotations['agents']['gilda'][1] is not None
assert len(annotations['agents']['gilda'][1]) == 2, \
annotations
# This is to make sure the to_json of the ScoredMatches works
assert annotations['agents']['gilda'][1][0]['term']['db'] == 'HGNC'
def test_grounding_map_gilda_priority():
gm.gilda_mode = 'web'
fetal_bovine_serum = Agent('FBS', db_refs={'TEXT': 'FBS'})
pmid = '28536624'
stmt = Phosphorylation(None, fetal_bovine_serum,
evidence=[Evidence(pmid=pmid,
text_refs={'PMID': pmid})])
mapped_stmts = gm.map_stmts([stmt])
annotations = mapped_stmts[0].evidence[0].annotations
# agents should not be in annotations if gilda is run. Second condition
# added as future proofing in case some future change causes this mapping
# to add agent annotations in the future.
assert 'agents' not in annotations or \
'gilda' not in annotations['agents']
def test_bound_condition_mapping_multi():
# Test with multiple agents
akt = Agent('pkbA', db_refs={'TEXT': 'Akt', 'UP': 'XXXXXX'})
erk = Agent('ERK1', db_refs={'TEXT': 'ERK1'})
akt.bound_conditions = [BoundCondition(erk)]
stmt = Phosphorylation(akt, erk)
mapped_stmts = gm.map_stmts([stmt])
s = mapped_stmts[0]
mapped_akt = mapped_stmts[0].enz
mapped_erk1 = mapped_akt.bound_conditions[0].agent
mapped_erk2 = mapped_stmts[0].sub
assert mapped_akt.db_refs['TEXT'] == 'Akt'
assert mapped_akt.db_refs['FPLX'] == 'AKT'
for e in (mapped_erk1, mapped_erk2):
assert e.db_refs['TEXT'] == 'ERK1'
assert e.db_refs['HGNC'] == '6877'
assert e.db_refs['UP'] == 'P27361'
def test_bound_condition_mapping_agent_json():
# Test with agent/json mapping
akt = Agent('pkbA', db_refs={'TEXT': 'p-Akt', 'UP': 'XXXXXX'})
erk = Agent('ERK1', db_refs={'TEXT': 'ERK1'})
akt.bound_conditions = [BoundCondition(erk)]
stmt = Phosphorylation(None, akt)
mapped_stmts = gm.map_stmts([stmt])
s = mapped_stmts[0]
mapped_akt = mapped_stmts[0].sub
mapped_erk = mapped_akt.bound_conditions[0].agent
#assert mapped_akt.db_refs['TEXT'] == 'p-AKT', mapped_akt.db_refs
assert mapped_akt.db_refs['FPLX'] == 'AKT', mapped_akt.db_refs
assert mapped_erk.db_refs['TEXT'] == 'ERK1'
assert mapped_erk.db_refs['HGNC'] == '6877'
assert mapped_erk.db_refs['UP'] == 'P27361'
def test_bound_condition_mapping():
# Verify that the grounding mapper grounds the agents within a bound
# condition
akt = Agent('pkbA', db_refs={'TEXT': 'Akt', 'UP': 'XXXXXX'})
erk = Agent('ERK1', db_refs={'TEXT': 'ERK1'})
akt.bound_conditions = [BoundCondition(erk)]
stmt = Phosphorylation(None, akt)
mapped_stmts = gm.map_stmts([stmt])
s = mapped_stmts[0]
mapped_akt = mapped_stmts[0].sub
mapped_erk = mapped_akt.bound_conditions[0].agent
assert mapped_akt.db_refs['TEXT'] == 'Akt'
assert mapped_akt.db_refs['FPLX'] == 'AKT'
assert mapped_erk.db_refs['TEXT'] == 'ERK1'
assert mapped_erk.db_refs['HGNC'] == '6877'
assert mapped_erk.db_refs['UP'] == 'P27361'
def test_none_text_corner_case():
ag = Agent('x', db_refs={'TEXT': None, 'TEXT_NORM': None})
stmt = Phosphorylation(None, ag)
res = gm.map_stmts([stmt])
assert res[0].sub.name == 'x', res[0]
def test_ignore():
agent = Agent('FA', db_refs={'TEXT': 'FA'})
stmt = Phosphorylation(None, agent)
mapped_stmts = gm.map_stmts([stmt])
assert len(mapped_stmts) == 0
def test_hgnc_sym_with_no_id():
erk = Agent('ERK1', db_refs={'TEXT': 'ERK1'})
stmt = Phosphorylation(None, erk)
g_map = {'ERK1': {'TEXT': 'ERK1', 'HGNC': 'foobar'}}
gm = GroundingMapper(g_map)
mapped_stmts = gm.map_stmts([stmt])
