Exemplo n.º 1
0

    blocks = explogparser.getBlocksFromExpLogJson(env['exp_json'], excludeThumbnail=True)
    dirs = ['block_%s' % block['id_str'] for block in blocks]
    if not is_composite:
        dirs=[]

    if args.do_sigproc:

        set_result_status('Merge Heatmaps')
        
        exclusionMaskFile = 'exclusionMask_%s.txt' % env.get('chipType','').lower()

        sigproc.mergeSigProcResults(
            dirs,
            env['SIGPROC_RESULTS'],
            env['shortRunName'],
            exclusionMaskFile)


    if args.do_basecalling:

        set_result_status('Merge Basecaller Results')

        # write composite return code
        try:
            composite_return_code=96
            for subdir in dirs:

                blockstatus_return_code_file = os.path.join(subdir,"blockstatus.txt")
                if os.path.exists(blockstatus_return_code_file):
Exemplo n.º 2
0
    number_of_total_blocks = len(blocks_to_process)
    dirs = ["block_%s" % block["id_str"] for block in blocks_to_process]
    if not is_composite:
        dirs = []

    if args.do_sigproc:

        set_result_status("Merge Heatmaps")

        # In 5.4 we apply a  mask in the bead find stage but also here to be backward compatible
        # with from-basecall reanalyses of 5.2 and older OIA results
        exclusionMaskFile = "exclusionMask_%s.txt" % env.get("chipType",
                                                             "").lower()

        sigproc.mergeSigProcResults(dirs, env["SIGPROC_RESULTS"],
                                    env["shortRunName"], exclusionMaskFile)
        """
        # write composite return code, not needed anymore ?
        try:
            composite_return_code=number_of_total_blocks
            for subdir in dirs:

                blockstatus_return_code_file = os.path.join(subdir,"blockstatus.txt")
                if os.path.exists(blockstatus_return_code_file):

                    with open(blockstatus_return_code_file, 'r') as f:
                        text = f.read()
                        if 'Analysis=0' in text:
                            composite_return_code-=1

            composite_return_code_file = os.path.join(SIGPROC_RESULTS,"analysis_return_code.txt")
Exemplo n.º 3
0
                printtime("MergeTLStatus %s\tpid %d\tpk file %s started" %
                          (status, os.getpid(), primary_key_file))
        except:
            traceback.print_exc()

    blocks = explogparser.getBlocksFromExpLogJson(env['exp_json'],
                                                  excludeThumbnail=True)
    dirs = ['block_%s' % block['id_str'] for block in blocks]

    if args.do_sigproc:

        set_result_status('Merge Heatmaps')
        merged_bead_mask_path = os.path.join(env['SIGPROC_RESULTS'],
                                             'MaskBead.mask')

        sigproc.mergeSigProcResults(dirs, env['SIGPROC_RESULTS'],
                                    env['shortRunName'])

    if args.do_basecalling:

        set_result_status('Merge Basecaller Results')

        try:
            sigproc.mergeRawPeakSignals(dirs)
        except:
            traceback.print_exc()

        try:
            # Only merge metrics and generate plots
            basecaller.merge_basecaller_stats(dirs, env['BASECALLER_RESULTS'],
                                              env['SIGPROC_RESULTS'],
                                              env['flows'], env['flowOrder'])
Exemplo n.º 4
0
    number_of_total_blocks = len(blocks_to_process)
    dirs = ['block_%s' % block['id_str'] for block in blocks_to_process]
    if not is_composite:
        dirs = []

    if args.do_sigproc:

        set_result_status('Merge Heatmaps')

        # In 5.4 we apply a  mask in the bead find stage but also here to be backward compatible
        # with from-basecall reanalyses of 5.2 and older OIA results
        exclusionMaskFile = 'exclusionMask_%s.txt' % env.get('chipType', '').lower()

        sigproc.mergeSigProcResults(
            dirs,
            env['SIGPROC_RESULTS'],
            env['shortRunName'],
            exclusionMaskFile)

        '''
        # write composite return code, not needed anymore ?
        try:
            composite_return_code=number_of_total_blocks
            for subdir in dirs:

                blockstatus_return_code_file = os.path.join(subdir,"blockstatus.txt")
                if os.path.exists(blockstatus_return_code_file):

                    with open(blockstatus_return_code_file, 'r') as f:
                        text = f.read()
                        if 'Analysis=0' in text:
Exemplo n.º 5
0
    env,warn = explogparser.getparameter()

    blockprocessing.write_version()
    sys.stdout.flush()
    sys.stderr.flush()

    blocks = explogparser.getBlocksFromExpLogJson(env['exp_json'], excludeThumbnail=True)
    dirs = ['block_%s' % block['id_str'] for block in blocks]


    if args.do_sigproc:

        merged_bead_mask_path = os.path.join(env['SIGPROC_RESULTS'], 'MaskBead.mask')

        sigproc.mergeSigProcResults(
            dirs,
            env['SIGPROC_RESULTS'],
            env['shortRunName'])


    if args.do_basecalling:
        # Only merge metrics and generate plots
        basecaller.merge_basecaller_stats(
            dirs,
            env['BASECALLER_RESULTS'],
            env['SIGPROC_RESULTS'],
            env['flows'],
            env['flowOrder'])
        ## Generate BaseCaller's composite "Big Data": unmapped.bam. Totally optional
        if env.get('libraryName','') == 'none':        
            actually_merge_unmapped_bams = True
        else:
Exemplo n.º 6
0
    blockprocessing.printheader()
    env = blockprocessing.getparameter()

    blockprocessing.write_version()
    sys.stdout.flush()
    sys.stderr.flush()

    blocks = blockprocessing.getBlocksFromExpLog(env['exp_json'], excludeThumbnail=True)
    dirs = ['block_%s' % block['id_str'] for block in blocks]


    if args.do_sigproc:

        sigproc.mergeSigProcResults(
            dirs,
            env['pathToRaw'],
            env['skipchecksum'],
            env['SIGPROC_RESULTS'])



    if args.do_basecalling:

        QualityPath = os.path.join(env['BASECALLER_RESULTS'], 'quality.summary')
        libsff = "%s/%s_%s.sff" % (env['BASECALLER_RESULTS'], env['expName'], env['resultsName'])
        tfsff = "%s/%s_%s.tf.sff" % (env['BASECALLER_RESULTS'], env['expName'], env['resultsName'])
        merged_bead_mask_path = os.path.join(env['SIGPROC_RESULTS'], 'MaskBead.mask')

        basecaller.mergeBasecallerResults(
            dirs,
            QualityPath,