Exemplo n.º 1
0
    def test_removal_cell_before_synapse_remove(self):
        """
        Removing whole cell after none of its content's reference is stored out of the cell - makes
        all its content and synapses removal.
        """
        cell2 = Cell(name="cell2")
        dend3 = cell2.add_sec("dend3", diam=10, l=10, nseg=10)
        cell2.add_synapse(source=None, mod_name="Exp2Syn", seg=dend3(0.5))
        dend3 = None

        cell2.remove_immediate_from_neuron()
        self.assertEqual(3, _get_secs())
    def setUpClass(cls):
        morpho_path = os.path.join(path, "..",
                                   "commons/morphologies/asc/cell2.asc")

        # Create cell
        cell = Cell(name="cell")
        cell.load_morpho(filepath=morpho_path)
        cell.insert("pas")
        cell.insert("hh")

        soma = cell.filter_secs("soma")
        dend = cell.filter_secs("apic[10]")
        syn = cell.add_synapse(source=None, mod_name="ExpSyn", seg=dend(0.5))

        # Prepare EPSP and AP (IClamp) protocols
        experiment = Experiment(iti=40)
        experiment.add_epsp(num=3,
                            synapse=syn,
                            init=20,
                            interval=20,
                            weight=0.02)
        experiment.add_iclamp(num=3,
                              segment=soma(0.5),
                              init=60,
                              interval=20,
                              dur=3,
                              amp=1.6)
        experiment.build()

        # Prepare plots
        rec = Record([soma(0.5), dend(0.5)], variables='v')

        # Run
        sim = Simulation(init_v=-70,
                         warmup=20,
                         with_neuron_gui=False,
                         constant_timestep=True)
        sim.run(runtime=100)

        cls.v_soma = rec.as_numpy('v', segment_name=soma(.5).name)
        cls.v_apic = rec.as_numpy('v', segment_name=dend(.5).name)

        syn.remove_immediate_from_neuron()
        soma.remove_immediate_from_neuron()
        dend.remove_immediate_from_neuron()
        rec.remove_immediate_from_neuron()
        experiment.remove_immediate_from_neuron()
        cell.remove_immediate_from_neuron()
        sim.remove_immediate_from_neuron()

        cls.sections_left = len(list(h.allsec()))
Exemplo n.º 3
0
from neuronpp.utils.record import Record
from neuronpp.utils.simulation import Simulation

path = os.path.dirname(os.path.abspath(__file__))

if __name__ == '__main__':
    # Prepare cell
    filepath = os.path.join(path, "..",
                            "commons/mods/sigma3syn")
    cell = Cell("cell", compile_paths=filepath)
    soma = cell.add_sec("soma", diam=20, l=20, nseg=10)
    cell.insert('pas')
    cell.insert('hh')

    w = 0.003  # LTP
    #w = 0.0022  # LTD
    syn = cell.add_synapse(source=None, netcon_weight=w, seg=soma(0.5), mod_name="ExcSigma3Exp2Syn")

    # prepare plots and spike detector
    rec_v = Record(soma(0.5), variables="v")
    rec_w = Record(syn, variables="w")

    # run
    sim = Simulation(init_v=-68, warmup=5)
    syn.make_event(5)
    sim.run(runtime=50)

    # plot
    rec_w.plot()
    rec_v.plot()
Exemplo n.º 4
0
class TestSection(unittest.TestCase):
    def setUp(self):
        self.cell = Cell(name="cell")
        self.soma = self.cell.add_sec("soma", diam=10, l=10, nseg=10)
        self.dend1 = self.cell.add_sec("dend1", diam=1, l=10, nseg=10)
        self.dend2 = self.cell.add_sec("dend2", diam=1, l=10, nseg=10)
        self.cell.connect_secs(child=self.dend1, parent=self.soma, child_loc=0, parent_loc=0.1)
        self.cell.connect_secs(child=self.dend2, parent=self.soma, child_loc=0, parent_loc=0.9)

    def tearDown(self):
        self.soma.remove_immediate_from_neuron()
        self.dend1.remove_immediate_from_neuron()
        self.dend2.remove_immediate_from_neuron()
        self.cell.remove_immediate_from_neuron()

        all_sec_num = _get_secs()
        if all_sec_num != 0:
            raise RuntimeError("Not all section have been removed after teardown. "
                               "Sections left: %s" % all_sec_num)

    def test_remove_synapse(self):
        """
        Removal of the parent section doesn't remove child sections
        """
        dend3 = self.cell.add_sec("dend3", diam=1, l=10, nseg=10)
        self.cell.connect_secs(child=dend3, parent=self.soma, child_loc=0, parent_loc=0.5)

        syn = self.cell.add_synapse(source=None, mod_name="Exp2Syn", seg=dend3(0.5))

        dend3 = None
        self.assertEqual(4, _get_secs())

        syn.remove_immediate_from_neuron()
        self.assertEqual(4, _get_secs())

    def test_remove_point_process(self):
        """
        Immediate removal of Point Process in outside reference makes
        that Point Process inside synapse also delete
        """
        syn = self.cell.add_synapse(source=None, mod_name="Exp2Syn", seg=self.dend2(0.5))
        syn.point_process.remove_immediate_from_neuron()
        self.assertEqual(0, len(self.cell.syns[0].point_process.__dict__))

    def test_remove_synapse2(self):
        """
        Remove outside reference and inside (Cell's) reference deletes section from NEURON
        """
        dend3 = self.cell.add_sec("dend3", diam=1, l=10, nseg=10)
        self.cell.connect_secs(child=dend3, parent=self.soma, child_loc=0, parent_loc=0.5)

        dend3 = None
        self.assertEqual(4, _get_secs())

        self.cell.secs = self.cell.secs[:3]
        self.assertEqual(3, len(self.cell.secs))
        self.assertEqual(3, _get_secs())

    def test_remove_synapse3(self):
        """
        Remove outside reference and inside (Cell's) reference doesn't delete section if it has
        reference inside the synapse
        """
        dend3 = self.cell.add_sec("dend3", diam=1, l=10, nseg=10)
        self.cell.connect_secs(child=dend3, parent=self.soma, child_loc=0, parent_loc=0.5)

        syn = self.cell.add_synapse(source=None, mod_name="Exp2Syn", seg=dend3(0.5))

        dend3 = None
        self.assertEqual(4, _get_secs())

        self.cell.secs = self.cell.secs[:3]
        self.assertEqual(3, len(self.cell.secs))
        self.assertEqual(4, _get_secs())

        syn.remove_immediate_from_neuron()
        self.assertEqual(3, _get_secs())

    def test_remove_soma(self):
        """
        Removal of the parent section doesn't remove child sections
        """
        self.soma.remove_immediate_from_neuron()
        self.assertEqual(2, _get_secs())

    def test_removal_sec_from_cell_object(self):
        """
        removal section by cell object - removes it from the cell object's self.secs list
        """
        self.cell.add_sec("dend3", diam=10, l=10, nseg=10)
        self.cell.remove_secs("dend3")
        self.assertEqual(3, _get_secs())
        self.assertEqual(3, len(self.cell.secs))

    def test_removal_sec_outside_cell_object(self):
        """
        removal section by itself - removes it from the cell object's self.secs list
        """
        dend3 = self.cell.add_sec("dend3", diam=10, l=10, nseg=10)
        dend3.remove_immediate_from_neuron()
        self.assertEqual(4, len(self.cell.secs))

    def test_removal_sec_outside_cell_object_clear_secs_manual(self):
        """
        removal section from secs clear also the object dend3
        """
        dend3 = self.cell.add_sec("dend3", diam=10, l=10, nseg=10)
        self.cell.secs.remove(dend3)
        self.assertEqual(3, len(self.cell.secs))

    def test_remove_dend1(self):
        """
        Removal of the parent section doesn't remove child sections
        """
        self.dend1.remove_immediate_from_neuron()
        self.assertEqual(2, _get_secs())

    def test_remove_dend1_soma(self):
        """
        Removal of the parent section doesn't remove child sections
        """
        self.soma.remove_immediate_from_neuron()
        self.dend1.remove_immediate_from_neuron()
        self.assertEqual(1, _get_secs())

    def test_remove_cell(self):
        """
        Removal of cell only doesn't remove any sections since their references are valid
        """
        self.cell.remove_immediate_from_neuron()
        self.assertEqual(3, _get_secs())

    def test_remove_sec_with_hoc_ref(self):
        """
        Immediate removal of Section doesn't remove it from NEURON if there is outside reference to
        the HOC object        that Point Process inside synapse also delete
        """
        hoc_soma = self.soma.hoc
        self.soma.remove_immediate_from_neuron()
        self.assertEqual(3, _get_secs())
        
    def test_remove_new_cell_with_no_ref(self):
        """
        deletion of cell, when all its sections have no reference outside - deletes all its
        sections as well
        """
        cell = Cell(name="cell")
        soma = cell.add_sec("soma")
        dend1 = cell.add_sec("dend1")
        dend2 = cell.add_sec("dend2")
        cell.connect_secs(child=dend1, parent=soma, child_loc=0, parent_loc=0.1)
        cell.connect_secs(child=dend2, parent=soma, child_loc=0, parent_loc=0.9)

        soma = None
        dend1 = None
        dend2 = None

        self.assertEqual(6, _get_secs())
        cell.remove_immediate_from_neuron()
        self.assertEqual(3, _get_secs())

    def test_remove_new_cell_with_del_ref(self):
        """
        deletion of cell, when all its outside references are deletes - deletes all its
        sections as well. But deletion of single soma won't delete section soma since its reference
        is still in Cell
        """
        cell = Cell(name="cell")
        soma = cell.add_sec("soma")
        dend1 = cell.add_sec("dend1")
        dend2 = cell.add_sec("dend2")
        cell.connect_secs(child=dend1, parent=soma, child_loc=0, parent_loc=0.1)
        cell.connect_secs(child=dend2, parent=soma, child_loc=0, parent_loc=0.9)

        del soma
        del dend1
        del dend2

        self.assertEqual(6, _get_secs())
        cell.remove_immediate_from_neuron()
        self.assertEqual(3, _get_secs())

    def test_remove_new_cell_with_remove_immediate(self):
        """
        remove immediate removes each section immediate only if there is no reference to the HOC
        object of this section outside the Sec object.
        """
        cell = Cell(name="cell")
        soma = cell.add_sec("soma")
        dend1 = cell.add_sec("dend1")
        dend2 = cell.add_sec("dend2")
        cell.connect_secs(child=dend1, parent=soma, child_loc=0, parent_loc=0.1)
        cell.connect_secs(child=dend2, parent=soma, child_loc=0, parent_loc=0.9)

        self.assertEqual(6, _get_secs())

        soma.remove_immediate_from_neuron()
        dend1.remove_immediate_from_neuron()
        dend2.remove_immediate_from_neuron()

        self.assertEqual(3, _get_secs())
        cell.remove_immediate_from_neuron()

    def test_removal_sec_after_synapse_remove(self):
        """
        Removing synapse allows to remove section as well
        """
        dend3 = self.cell.add_sec("dend3", diam=10, l=10, nseg=10)
        syn = self.cell.add_synapse(source=None, mod_name="Exp2Syn", seg=dend3(0.5))
        syn.remove_immediate_from_neuron()
        self.soma.remove_immediate_from_neuron()
        self.assertEqual(3, _get_secs())

    def test_removal_sec_as_part_of_synapse(self):
        """
        Removal of Sec which is a part of a Synapse won't delete the Section in NEURON as long as
        Synapse exists.
        """
        dend3 = self.cell.add_sec("dend3", diam=10, l=10, nseg=10)
        syn = self.cell.add_synapse(source=None, mod_name="Exp2Syn", seg=dend3(0.5))
        dend3.remove_immediate_from_neuron()
        self.assertEqual(4, _get_secs())

    def test_removal_sec_before_synapse_remove(self):
        """
        Removing section before removing synapse - makes that section is not removed. It is because
        we remove dend3 section, but self.cell still holds the reference to the synapse.
        """
        dend3 = self.cell.add_sec("dend3", diam=10, l=10, nseg=10)
        self.cell.add_synapse(source=None, mod_name="Exp2Syn", seg=dend3(0.5))
        dend3.remove_immediate_from_neuron()
        self.assertEqual(4, _get_secs())

    def test_removal_cell_before_synapse_remove(self):
        """
        Removing whole cell after none of its content's reference is stored out of the cell - makes
        all its content and synapses removal.
        """
        cell2 = Cell(name="cell2")
        dend3 = cell2.add_sec("dend3", diam=10, l=10, nseg=10)
        cell2.add_synapse(source=None, mod_name="Exp2Syn", seg=dend3(0.5))
        dend3 = None

        cell2.remove_immediate_from_neuron()
        self.assertEqual(3, _get_secs())
Exemplo n.º 5
0
class TestSimulation(unittest.TestCase):

    def setUp(self):
        """
        Setup call after each test, however from the NEURON point of view - all created HOC
        objects exist, so each new call of setUp() creates a separated new cell on which tests are
        performed
        """
        morpho_path = os.path.join(path, "..", "commons/morphologies/asc/cell2.asc")
        self.cell = Cell(name="cell")
        self.cell.load_morpho(filepath=morpho_path)
        self.cell.insert("pas")
        self.cell.insert("hh")

        self.soma = self.cell.filter_secs("soma")
        self.apic1 = self.cell.filter_secs('apic[1]')

    def tearDown(self):
        self.soma.remove_immediate_from_neuron()
        self.apic1.remove_immediate_from_neuron()
        self.cell.remove_immediate_from_neuron()

        l = len(list(h.allsec()))
        if len(list(h.allsec())) != 0:
            raise RuntimeError("Not all section have been removed after teardown. "
                               "Sections left: %s" % l)

    def test_empty_method(self):
        sim = Simulation()
        self.assertFalse(sim.is_neuron_empty())

        self.soma.remove_immediate_from_neuron()
        self.apic1.remove_immediate_from_neuron()
        self.cell.remove_immediate_from_neuron()

        self.assertTrue(sim.is_neuron_empty())

    def test_size_method(self):
        sim = Simulation()
        self.assertEqual(198, sim.size)

        self.soma.remove_immediate_from_neuron()
        self.apic1.remove_immediate_from_neuron()
        self.cell.remove_immediate_from_neuron()

        self.assertEqual(0, sim.size)

    def test_dt(self):
        rec = Record(self.soma(0.5))

        sim = Simulation(dt=10)
        sim.run(100)
        r1 = rec.as_numpy('v')

        sim = Simulation(dt=0.01)
        sim.run(100)
        r2 = rec.as_numpy('v')

        sim.remove_immediate_from_neuron()
        rec.remove_immediate_from_neuron()

        self.assertEqual(11, r1.size)
        self.assertEqual(10001, r2.size)

    def test_init_v(self):
        rec = Record(self.soma(0.5))

        sim = Simulation(init_v=-100)
        sim.run(100)
        r1 = rec.as_numpy('v')

        sim = Simulation(init_v=100)
        sim.run(100)
        r2 = rec.as_numpy('v')

        sim.remove_immediate_from_neuron()
        rec.remove_immediate_from_neuron()

        self.assertEqual(-100, r1.records[0])
        self.assertEqual(100, r2.records[0])

    def test_timestep_constant(self):
        rec = Record(self.soma(0.5))

        sim = Simulation(constant_timestep=True, dt=1)
        sim.run(100)
        r1 = rec.as_numpy('v')

        sim = Simulation(constant_timestep=False, dt=1)
        sim.run(100)
        r2 = rec.as_numpy('v')

        sim = Simulation(constant_timestep=False, dt=10)
        sim.run(100)
        r3 = rec.as_numpy('v')

        sim = Simulation(constant_timestep=False, dt=0.0001)
        sim.run(100)
        r4 = rec.as_numpy('v')

        sim.remove_immediate_from_neuron()
        rec.remove_immediate_from_neuron()

        self.assertEqual(101, r1.size)
        self.assertEqual(188, r2.size)
        self.assertEqual(180, r3.size)
        self.assertEqual(189, r4.size)

    def test_warmup(self):
        rec = Record(self.soma(0.5))

        value_error = False
        try:
            sim = Simulation(warmup=-100)
            sim.run(100)
            r1 = rec.as_numpy('v')
        except ValueError:
            value_error = True
        self.assertTrue(value_error)

        sim = Simulation(warmup=100, dt=1)
        sim.run(100)
        r2 = rec.as_numpy('v')

        sim = Simulation(warmup=100, dt=1, warmup_dt=1)
        sim.run(100)
        r3 = rec.as_numpy('v')

        sim.remove_immediate_from_neuron()
        rec.remove_immediate_from_neuron()

        self.assertEqual(111, r2.size)
        self.assertEqual(201, r3.size)

    def test_netstim_before_sim(self):
        """
        NetStim run in a regular way before Simulation run, in this cale (netcon weight=1.0)
        we have spike of the soma.

        However NetStim start is in the absolute time.
        """
        # Netstim to synapse
        stim = NetStimCell("stim").add_netstim(start=25, number=10, interval=2)
        self.cell.add_synapse(source=stim, netcon_weight=1.0, mod_name="ExpSyn", delay=1,
                              seg=self.apic1(0.5))
        # Record
        rec = Record(self.soma(0.5))
        # Run
        sim = Simulation(init_v=-70, warmup=20)
        sim.run(1)

        sim.run(100)
        r = rec.as_numpy(variable="v")

        stim.remove_immediate_from_neuron()
        sim.remove_immediate_from_neuron()
        rec.remove_immediate_from_neuron()

        # Make assertions
        self.assertEqual(4051, r.size)
        self.assertEqual(34.5205, round(r.records.max(), 4))
        self.assertEqual(-75.3053, round(r.records.min(), 4))

        self.assertEqual(319, r.records.argmax())
        # time in ms of max mV value
        self.assertEqual(27.725, round(r.time[r.records.argmax()], 4))

    def test_netcon_event_before_sim(self):
        """
        Netcon created before sim init or start is not effective and should return error
        """
        sim = Simulation()
        sim.reinit()
        syn = self.cell.add_synapse(source=None, netcon_weight=1.0, mod_name="ExpSyn", delay=1,
                                    seg=self.apic1(0.5))
        error = False
        try:
            syn.make_event(50)
        except ConnectionRefusedError:
            error = True

        self.assertTrue(error)

    def test_netcon_event_after_sim(self):
        syn = self.cell.add_synapse(source=None, netcon_weight=1.0, mod_name="ExpSyn", delay=1,
                                    seg=self.apic1(0.5))
        # Record
        rec = Record(self.soma(0.5))
        # Run
        sim = Simulation(init_v=-70, warmup=20)
        sim.run(1)

        syn.make_event(50)

        sim.run(100)
        r = rec.as_numpy(variable="v")

        syn.remove_immediate_from_neuron()
        sim.remove_immediate_from_neuron()
        rec.remove_immediate_from_neuron()

        # Make assertions
        self.assertEqual(4051, r.size)
        self.assertEqual(34.4582, round(r.records.max(), 4))
        self.assertEqual(-75.3478, round(r.records.min(), 4))

        self.assertEqual(2159, r.records.argmax())
        # time in ms of max mV value
        self.assertEqual(73.725, round(r.time[r.records.argmax()], 4))

    def test_netstim_after_sim(self):
        """
        NetStim created after simulation run has no effect, it won't go on this simulation at all.

        However NetStim start is in the absolute time.
        """
        # Record
        rec = Record(self.soma(0.5))
        # Run
        sim = Simulation(init_v=-70, warmup=20)
        sim.run(1)

        # Netstim to synapse
        stim = NetStimCell("stim").add_netstim(start=25, number=10, interval=2)
        self.cell.add_synapse(source=stim, netcon_weight=0.5, mod_name="ExpSyn", delay=1,
                              seg=self.apic1(0.5))
        sim.run(100)
        r = rec.as_numpy(variable="v")

        # Make assertions
        self.assertEqual(4051, r.size)
        self.assertEqual(-67.1917, round(r.records.max(), 4))
        self.assertEqual(-70.0, round(r.records.min(), 4))

        self.assertEqual(3, r.records.argmax())
        # time in ms of max mV value
        self.assertEqual(6, r.time[r.records.argmax()])

        stim.remove_immediate_from_neuron()
        sim.remove_immediate_from_neuron()
        rec.remove_immediate_from_neuron()

    def test_iclamp_before_sim(self):
        """
        IClamp works in a regular way before simulation run. in this case (amplitude 3 pA)
        we have spike at the soma.

        However IClamp delay is in the absolute time.
        """
        # Record
        rec = Record(self.soma(0.5))

        # IClamp to soma
        iclamp = IClamp(segment=self.soma(0.5))
        iclamp.stim(delay=25, dur=3, amp=3)

        # Run
        sim = Simulation(init_v=-70, warmup=20)
        sim.run(1)

        sim.run(100)
        r = rec.as_numpy(variable="v")

        sim.remove_immediate_from_neuron()
        rec.remove_immediate_from_neuron()
        iclamp.remove_immediate_from_neuron()

        # Make assertions
        self.assertEqual(4051, r.size)
        self.assertEqual(34.3815, round(r.records.max(), 4))
        self.assertEqual(-75.3247, round(r.records.min(), 4))

        self.assertEqual(330, r.records.argmax())
        # time in ms of max mV value
        self.assertEqual(28, round(r.time[r.records.argmax()], 4))

    def test_iclamp_after_sim(self):
        """
        IClamp works in a regular way after simulation run. in this case (amplitude 3 pA)
        we have spike at the soma.

        However IClamp delay is in the absolute time.
        """
        # Record
        rec = Record(self.soma(0.5))

        # Run
        sim = Simulation(init_v=-70, warmup=20)
        sim.run(1)

        # IClamp to soma
        iclamp = IClamp(segment=self.soma(0.5))
        iclamp.stim(delay=25, dur=3, amp=3)

        sim.run(100)
        r = rec.as_numpy(variable="v")

        sim.remove_immediate_from_neuron()
        rec.remove_immediate_from_neuron()
        iclamp.remove_immediate_from_neuron()

        # Make assertions
        self.assertEqual(4051, r.size)
        self.assertEqual(34.3815, round(r.records.max(), 4))
        self.assertEqual(-75.3247, round(r.records.min(), 4))

        self.assertEqual(330, r.records.argmax())
        # time in ms of max mV value
        self.assertEqual(28, round(r.time[r.records.argmax()], 4))

    def test_2_runs(self):
        """
        Test setup for 2 cells and their records which should be the same.

        After each creation of the Simulation object, the Record object is cleaned up
        (inside vector is empty) however on other Hoc object is removed eg. Sections.
        That means - each new sections and cell are retained in the current NEURON run.
        """

        def run_and_get_rec():
            rec = Record(self.soma(0.5))
            iclamp = IClamp(segment=self.soma(0.5))
            iclamp.stim(delay=25, dur=3, amp=3)
            sim = Simulation(init_v=-70, warmup=20)
            sim.run(100)
            return rec

        rec1 = run_and_get_rec()
        r1 = rec1.as_numpy('v').records

        self.tearDown()
        self.setUp()

        rec2 = run_and_get_rec()
        r2 = rec2.as_numpy('v').records

        sim = Simulation(init_v=-70, warmup=20)
        sim.run(100)

        # Make assertions
        self.assertEqual(4011, r1.size)
        self.assertEqual(4011, r2.size)
        self.assertTrue(np.alltrue(r1 == r2))

    def test_record_before_sim(self):
        """
        Record created before simulation run is full of data
        """
        # Record
        rec = Record(self.soma(0.5))

        # IClamp to soma
        iclamp = IClamp(segment=self.soma(0.5))
        iclamp.stim(delay=25, dur=3, amp=3)

        # Run
        sim = Simulation(init_v=-70, warmup=20)
        sim.run(1)
        print(h.t)

        sim.run(100)
        r = rec.as_numpy(variable="v")

        # Make assertions
        self.assertEqual(4051, r.size)
        self.assertEqual(34.3815, round(r.records.max(), 4))
        self.assertEqual(-75.3247, round(r.records.min(), 4))

        self.assertEqual(330, r.records.argmax())
        # time in ms of max mV value
        self.assertEqual(28, round(r.time[r.records.argmax()], 4))

    def test_record_after_sim(self):
        """
        record created after simulation run is empty
        """
        # IClamp to soma
        iclamp = IClamp(segment=self.soma(0.5))
        iclamp.stim(delay=25, dur=3, amp=3)

        # Run
        sim = Simulation(init_v=-70, warmup=20)
        sim.run(1)
        print(h.t)

        # Record
        rec = Record(self.soma(0.5))

        sim.run(100)
        r = rec.as_numpy(variable="v")

        # Make assertion
        self.assertEqual(0, r.size)
Exemplo n.º 6
0
cell.insert("pas")
cell.insert("hh")

# Two examples of synapses with NetStim:
stim_cell = NetStimCell("stim_cell")
stim = stim_cell.make_netstim(start=250, number=30, interval=1)
soma = cell.filter_secs("soma")

# 1) Hoc-style synapse
pp = cell.add_point_process(mod_name="ExpSyn", seg=soma(0.5))
cell.add_netcon(source=stim, point_process=pp, netcon_weight=0.01, delay=1)

# 2) Recommended synapse
syn1 = cell.add_synapse(source=stim,
                        seg=soma(0.5),
                        netcon_weight=0.01,
                        mod_name="Syn4P",
                        delay=1)

# 3) Event synapse
syn2 = cell.add_synapse(source=None,
                        seg=soma(0.5),
                        netcon_weight=0.01,
                        mod_name="Syn4P",
                        delay=1)

# prepare plots
rec_v = Record(soma(0.5), variables="v")

# run
sim = Simulation(init_v=-55, warmup=20)
Exemplo n.º 7
0
from neuronpp.cells.cell import Cell
from neuronpp.utils.record import Record
from neuronpp.utils.experiment import Experiment
from neuronpp.utils.simulation import Simulation

# Create cell
cell = Cell(name="cell")
cell.load_morpho(filepath="../commons/morphologies/asc/cell2.asc")
cell.insert("pas")
cell.insert("hh")

soma = cell.filter_secs("soma")
dend = cell.filter_secs("apic[10]")
syn = cell.add_synapse(source=None, mod_name="ExpSyn", seg=dend(0.5))

# Prepare EPSP and AP (IClamp) protocols
experiment = Experiment(iti=40)
experiment.add_epsp(num=3, synapse=syn, init=20, interval=20, weight=0.02)
experiment.add_iclamp(num=3,
                      segment=soma(0.5),
                      init=60,
                      interval=20,
                      dur=3,
                      amp=1.6)
experiment.build()

# Prepare plots
rec = Record([soma(0.5), dend(0.5)], variables='v')

# Run
sim = Simulation(init_v=-70,
Exemplo n.º 8
0
from neuronpp.cells.cell import Cell

from neuronpp.utils.synaptic_debugger import SynapticDebugger


if __name__ == '__main__':
    # Prepare cell
    cell = Cell("cell")
    soma = cell.add_sec("soma", diam=20, l=20, nseg=100)
    cell.insert("pas")
    cell.insert("hh")

    syn1 = cell.add_synapse(source=None, netcon_weight=0.002, seg=soma(0.1), mod_name="Exp2Syn")
    syn2 = cell.add_synapse(source=None, netcon_weight=0.002, seg=soma(0.9), mod_name="Exp2Syn")
    syn3 = cell.add_synapse(source=None, netcon_weight=0.002, seg=soma(0.5), mod_name="Exp2Syn")

    # Debug
    debug = SynapticDebugger(init_v=-70, warmup=10, delay_between_steps=15)
    debug.add_syn(syn1, key_press='1', plot=False)
    debug.add_syn(syn2, key_press='2', plot=False)
    debug.add_syn(syn3, key_press='3', plot=False)
    debug.add_seg(soma(0.5))
    debug.debug_interactive()